Base de dados : MEDLINE Pesquisa : B01.050.150.900.649.573.575.150 [Categoria DeCS] Referências encontradas : 138 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 29256285 [Au] Autor: Carnevali V; Nogueda-Torres B; Villagrán-Herrera ME; De Diego-Cabrera JA; Rocha-Chávez G; Martínez-Ibarra JA [Ad] Endereço: 1 Department of Public Health and Infectious Diseases, University of Rome , Rome , Italy. [Ti] Título: Prevalence of Trypanosoma cruzi and organ alterations in Virginia opossums (Didelphis virginiana) from western Mexico - short communication. [So] Source: Acta Vet Hung;65(4):505-509, 2017 12. [Is] ISSN: 0236-6290 [Cp] País de publicação: Hungary [La] Idioma: eng [Ab] Resumo: Small populations of Virginia opossum (Didelphis virginiana) in western Mexico are endangered by hunting and natural predators as well as by different kinds of diseases. After two serological analyses using Serodia® latex particle agglutination and indirect haemagglutination (IHA) tests, 35 (53.03%) of 66 collected opossums in two small towns in western Mexico were positive for the presence of Trypanosoma cruzi. Twenty-eight of the 35 seropositive opossums had pathological lesions: 11 had changes in only one organ, 13 in two organs, and four had pathological changes in three organs. Splenomegaly was the most common finding in the examined opossums, followed by hepatomegaly. These potentially fatal pathological changes could contribute to the scarcity of the opossum population, even leading to the extinction of this species in western Mexico. [Mh] Termos MeSH primário: Didelphis/parasitologia Trypanosoma cruzi/isolamento & purificação Tripanossomíase/veterinária [Mh] Termos MeSH secundário: Animais Cardiomegalia/epidemiologia Cardiomegalia/parasitologia Cardiomegalia/veterinária Acalasia Esofágica/epidemiologia Acalasia Esofágica/parasitologia Acalasia Esofágica/veterinária Hepatomegalia/epidemiologia Hepatomegalia/parasitologia Hepatomegalia/veterinária México/epidemiologia Esplenomegalia/epidemiologia Esplenomegalia/parasitologia Esplenomegalia/veterinária Tripanossomíase/epidemiologia Tripanossomíase/patologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180305 [Lr] Data última revisão: 180305 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171220 [St] Status: MEDLINE [do] DOI: 10.1556/004.2017.048

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[PMID]: 29211107 [Au] Autor: Lopes CMT; Menna-Barreto RFS; Pavan MG; Pereira MCS; Roque ALR [Ad] Endereço: Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia de Tripanosomatídeos, Rio de Janeiro, RJ, Brasil. [Ti] Título: Trypanosoma janseni n. sp. (Trypanosomatida: Trypanosomatidae) isolated from Didelphis aurita (Mammalia: Didelphidae) in the Atlantic Rainforest of Rio de Janeiro, Brazil: integrative taxonomy and phylogeography within the Trypanosoma cruzi clade. [So] Source: Mem Inst Oswaldo Cruz;113(1):45-55, 2018 Jan. [Is] ISSN: 1678-8060 [Cp] País de publicação: Brazil [La] Idioma: eng [Ab] Resumo: BACKGROUND: Didelphis spp. are a South American marsupial species that are among the most ancient hosts for the Trypanosoma spp. OBJECTIVES: We characterise a new species (Trypanosoma janseni n. sp.) isolated from the spleen and liver tissues of Didelphis aurita in the Atlantic Rainforest of Rio de Janeiro, Brazil. METHODS: The parasites were isolated and a growth curve was performed in NNN and Schneider's media containing 10% foetal bovine serum. Parasite morphology was evaluated via light microscopy on Giemsa-stained culture smears, as well as scanning and transmission electron microscopy. Molecular taxonomy was based on a partial region (737-bp) of the small subunit (18S) ribosomal RNA gene and 708 bp of the nuclear marker, glycosomal glyceraldehyde-3-phosphate dehydrogenase (gGAPDH) genes. Maximum likelihood and Bayesian inference methods were used to perform a species coalescent analysis and to generate individual and concatenated gene trees. Divergence times among species that belong to the T. cruzi clade were also inferred. FINDINGS: In vitro growth curves demonstrated a very short log phase, achieving a maximum growth rate at day 3 followed by a sharp decline. Only epimastigote forms were observed under light and scanning microscopy. Transmission electron microscopy analysis showed structures typical to Trypanosoma spp., except one structure that presented as single-membraned, usually grouped in stacks of three or four. Phylogeography analyses confirmed the distinct species status of T. janseni n. sp. within the T. cruzi clade. Trypanosoma janseni n. sp. clusters with T. wauwau in a well-supported clade, which is exclusive and monophyletic. The separation of the South American T. wauwau + T. janseni coincides with the separation of the Southern Super Continent. CONCLUSIONS: This clade is a sister group of the trypanosomes found in Australian marsupials and its discovery sheds light on the initial diversification process based on what we currently know about the T. cruzi clade. [Mh] Termos MeSH primário: DNA de Protozoário/genética Didelphis/parasitologia RNA Ribossômico 18S/genética Trypanosomatina/genética [Mh] Termos MeSH secundário: Animais Brasil Filogeografia Reação em Cadeia da Polimerase Floresta Úmida Trypanosoma cruzi Trypanosomatina/classificação Trypanosomatina/isolamento & purificação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Protozoan); 0 (RNA, Ribosomal, 18S) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180131 [Lr] Data última revisão: 180131 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171207 [St] Status: MEDLINE

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[PMID]: 28768665 [Au] Autor: Hu MC; Bobulescu IA; Quiñones H; Gisler SM; Moe OW [Ad] Endereço: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas; Ming-Chang.Hu@UTSouthwestern.edu. [Ti] Título: Dopamine reduces cell surface Na /H exchanger-3 protein by decreasing NHE3 exocytosis and cell membrane recycling. [So] Source: Am J Physiol Renal Physiol;313(4):F1018-F1025, 2017 Oct 01. [Is] ISSN: 1522-1466 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The intrarenal autocrine-paracrine dopamine (DA) system mediates a significant fraction of the natriuresis in response to a salt load. DA inhibits a number of Na transporters to effect sodium excretion, including the proximal tubule Na /H exchanger-3 (NHE3). DA represent a single hormone that regulates NHE3 at multiple levels, including translation, degradation, endocytosis, and protein phosphorylation. Because cell surface NHE3 protein is determined by the balance between exocytotic insertion and endocytotic retrieval, we examined whether DA acutely affects the rate of NHE3 exocytosis in a cell culture model. DA inhibited NHE3 exocytosis at a dose-dependent manner with a half maximal around 10 M. The DA effect on NHE3 exocytosis was blocked by inhibition of protein kinase A and by brefeldin A, which inhibits endoplasmic reticulum-to-Golgi transport. NHE3 directly interacts with the ε-subunit of coatomer protein based on yeast-two-hybrid and coimmunoprecipitation. Because NHE3 has been shown to be recycled back to the cell membrane after endocytosis, we measured NHE3 recycling using a biochemical reinsertion assay and showed that reinsertion of NHE3 back to the membrane is also inhibited by DA. In conclusion, among the many mechanisms by which DA reduces apical membrane NHE3 and induces proximal tubule natriuresis, one additional mechanism is inhibition of exocytotic insertion and reinsertion of NHE3 in the apical cell surface. [Mh] Termos MeSH primário: Membrana Celular/efeitos dos fármacos Dopamina/farmacologia Exocitose/efeitos dos fármacos Rim/efeitos dos fármacos Trocadores de Sódio-Hidrogênio/metabolismo [Mh] Termos MeSH secundário: Animais Membrana Celular/metabolismo Células Cultivadas Didelphis Relação Dose-Resposta a Droga Regulação para Baixo Rim/metabolismo Natriurese/efeitos dos fármacos Transporte Proteico Trocador 3 de Sódio-Hidrogênio Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Sodium-Hydrogen Exchanger 3); 0 (Sodium-Hydrogen Exchangers); VTD58H1Z2X (Dopamine) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170804 [St] Status: MEDLINE [do] DOI: 10.1152/ajprenal.00251.2017

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[PMID]: 28422973 [Au] Autor: O'Connor KM; Nathan LR; Liberati MR; Tingley MW; Vokoun JC; Rittenhouse TAG [Ad] Endereço: Wildlife and Fisheries Conservation Center, Department of Natural Resources and the Environment, University of Connecticut, Connecticut, United States of America. [Ti] Título: Camera trap arrays improve detection probability of wildlife: Investigating study design considerations using an empirical dataset. [So] Source: PLoS One;12(4):e0175684, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Camera trapping is a standard tool in ecological research and wildlife conservation. Study designs, particularly for small-bodied or cryptic wildlife species often attempt to boost low detection probabilities by using non-random camera placement or baited cameras, which may bias data, or incorrectly estimate detection and occupancy. We investigated the ability of non-baited, multi-camera arrays to increase detection probabilities of wildlife. Study design components were evaluated for their influence on wildlife detectability by iteratively parsing an empirical dataset (1) by different sizes of camera arrays deployed (1-10 cameras), and (2) by total season length (1-365 days). Four species from our dataset that represented a range of body sizes and differing degrees of presumed detectability based on life history traits were investigated: white-tailed deer (Odocoileus virginianus), bobcat (Lynx rufus), raccoon (Procyon lotor), and Virginia opossum (Didelphis virginiana). For all species, increasing from a single camera to a multi-camera array significantly improved detection probability across the range of season lengths and number of study sites evaluated. The use of a two camera array increased survey detection an average of 80% (range 40-128%) from the detection probability of a single camera across the four species. Species that were detected infrequently benefited most from a multiple-camera array, where the addition of up to eight cameras produced significant increases in detectability. However, for species detected at high frequencies, single cameras produced a season-long (i.e, the length of time over which cameras are deployed and actively monitored) detectability greater than 0.75. These results highlight the need for researchers to be critical about camera trap study designs based on their intended target species, as detectability for each focal species responded differently to array size and season length. We suggest that researchers a priori identify target species for which inference will be made, and then design camera trapping studies around the most difficult to detect of those species. [Mh] Termos MeSH primário: Cervos/fisiologia Didelphis/fisiologia Lynx/fisiologia Fotografia/métodos Guaxinins/fisiologia [Mh] Termos MeSH secundário: Distribuição Animal Animais Connecticut Fotografia/instrumentação Projetos de Pesquisa Análise Espaço-Temporal Gravação em Vídeo [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170505 [Lr] Data última revisão: 170505 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170420 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0175684

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[PMID]: 28375825 [Au] Autor: Pope JP; Donnell RL [Ad] Endereço: Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN. [Ti] Título: Spontaneous neoplasms in captive Virginia opossums ( Didelphis virginiana): a retrospective case series (1989-2014) and review of the literature. [So] Source: J Vet Diagn Invest;29(3):331-337, 2017 May. [Is] ISSN: 1943-4936 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: This retrospective project summarizes the types of neoplasms identified in Virginia opossums ( Didelphis virginiana) presented to the University of Tennessee, College of Veterinary Medicine (UTCVM) postmortem service in 1989-2014 and serves as a review of the literature. Of the 85 Virginia opossums identified from the UTCVM case database, there were 17 diagnoses of neoplasia from 12 cases (14%). These cases included 8 females, 2 males, and 2 neutered males. All opossums with known ages (11 of 12) were >2 y old. Pulmonary tumors, specifically minimally invasive or lepidic-predominant adenocarcinomas, were the most common diagnosis and accounted for 53% (9 of 17) of the neoplasms. Additional tumors included acute myeloid leukemia with eosinophil maturation, hepatic hemangiosarcoma, sarcoma (unknown origin), squamous cell carcinoma, disseminated mast cell tumor, trichoblastoma, thyroid adenoma, and an osteoma. These findings serve as a reference for the types of spontaneous neoplasms in Virginia opossums; based on these findings, neoplasia should be considered as a differential in mature captive Virginia opossums. [Mh] Termos MeSH primário: Carcinoma de Células Escamosas/veterinária Didelphis Neoplasias Pulmonares/veterinária Neoplasias Cutâneas/veterinária [Mh] Termos MeSH secundário: Animais Animais Selvagens Carcinoma de Células Escamosas/diagnóstico Carcinoma de Células Escamosas/epidemiologia Feminino Neoplasias Pulmonares/diagnóstico Neoplasias Pulmonares/epidemiologia Masculino Estudos Retrospectivos Neoplasias Cutâneas/diagnóstico Neoplasias Cutâneas/epidemiologia Tennessee/epidemiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170713 [Lr] Data última revisão: 170713 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170405 [St] Status: MEDLINE [do] DOI: 10.1177/1040638717704017

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[PMID]: 28012573 [Au] Autor: Dos Santos ÍG; Jorge EC; Copola AG; Bertassoli BM; Goes AM; Silva GA [Ad] Endereço: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Morfologia, Avenida Presidente Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address: iriagds@ufmg.br. [Ti] Título: FGF2, FGF3 and FGF4 expression pattern during molars odontogenesis in Didelphis albiventris. [So] Source: Acta Histochem;119(2):129-141, 2017 Mar. [Is] ISSN: 1618-0372 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Odontogenesis is guided by a complex signaling cascade in which several molecules, including FGF2-4, ensure all dental groups development and specificity. Most of the data on odontogenesis derives from rodents, which does not have all dental groups. Didelphis albiventris is an opossum with the closest dentition to humans, and the main odontogenesis stages occur when the newborns are in the pouch. In this study, D. albiventris postnatals were used to characterize the main stages of their molars development; and also to establish FGF2, FGF3 and FGF4 expression pattern. D. albiventris postnatals were processed for histological and indirect immunoperoxidase analysis of the tooth germs. Our results revealed similar dental structures between D. albiventris and mice. However, FGF2, FGF3 and FGF4 expression patterns were observed in a larger number of dental structures, suggesting broader functions for these molecules in this opossum species. The knowledge of the signaling that determinates odontogenesis in an animal model with complete dentition may contribute to the development of therapies for the replacement of lost teeth in humans. This study may also contribute to the implementation of D. albiventris as model for Developmental Biology studies. [Mh] Termos MeSH primário: Didelphis/metabolismo Fator 2 de Crescimento de Fibroblastos/metabolismo Fator 3 de Crescimento de Fibroblastos/metabolismo Fator 4 de Crescimento de Fibroblastos/metabolismo Dente Molar/crescimento & desenvolvimento Odontogênese [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Sequência Conservada Didelphis/crescimento & desenvolvimento Feminino Fator 2 de Crescimento de Fibroblastos/genética Fator 3 de Crescimento de Fibroblastos/genética Fator 4 de Crescimento de Fibroblastos/genética Camundongos Dente Molar/citologia Dente Molar/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fibroblast Growth Factor 3); 0 (Fibroblast Growth Factor 4); 103107-01-3 (Fibroblast Growth Factor 2) [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 170221 [Lr] Data última revisão: 170221 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161226 [St] Status: MEDLINE

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[PMID]: 27931926 [Au] Autor: Lavelle MJ; Kay SL; Pepin KM; Grear DA; Campa H; VerCauteren KC [Ad] Endereço: United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, CO 80521-2154, USA. Electronic address: michael.j.lavelle@aphis.usda.gov. [Ti] Título: Evaluating wildlife-cattle contact rates to improve the understanding of dynamics of bovine tuberculosis transmission in Michigan, USA. [So] Source: Prev Vet Med;135:28-36, 2016 Dec 01. [Is] ISSN: 1873-1716 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Direct and indirect contacts among individuals drive transmission of infectious disease. When multiple interacting species are susceptible to the same pathogen, risk assessment must include all potential host species. Bovine tuberculosis (bTB) is an example of a disease that can be transmitted among several wildlife species and to cattle, although the potential role of several wildlife species in spillback to cattle remains unclear. To better understand the complex network of contacts and factors driving disease transmission, we fitted proximity logger collars to beef and dairy cattle (n=37), white-tailed deer (Odocoileus virginianus; n=29), raccoon (Procyon lotor; n=53), and Virginia opossum (Didelphis virginiana; n=79) for 16 months in Michigan's Lower Peninsula, USA. We determined inter- and intra-species direct and indirect contact rates. Data on indirect contact was calculated when collared animals visited stationary proximity loggers placed at cattle feed and water resources. Most contact between wildlife species and cattle was indirect, with the highest contact rates occurring between raccoons and cattle during summer and fall. Nearly all visits (>99%) to cattle feed and water sources were by cattle, whereas visitation to stored cattle feed was dominated by deer and raccoon (46% and 38%, respectively). Our results suggest that indirect contact resulting from wildlife species visiting cattle-related resources could pose a risk of disease transmission to cattle and deserves continued attention with active mitigation. [Mh] Termos MeSH primário: Distribuição Animal Animais Selvagens Reservatórios de Doenças/veterinária Mycobacterium bovis/fisiologia Tuberculose Bovina/transmissão [Mh] Termos MeSH secundário: Animais Bovinos Cervos Didelphis Michigan Guaxinins Tuberculose Bovina/microbiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170911 [Lr] Data última revisão: 170911 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161210 [St] Status: MEDLINE

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[PMID]: 27692317 [Au] Autor: da Costa Neto SF; de Oliveira Simões R; Mota ÉM; do Val Vilela R; Lopes Torres EJ; Santos Barbosa H; Gentile R; Maldonado Junior A [Ad] Endereço: Programa de Pós graduação em Biodiversidade e Saúde, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, RJ, 21040-360, Brazil; Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, R [Ti] Título: Lungworm Heterostrongylus heterostrongylus Travassos, 1925 from the black-eared opossum in South America: Morphologic, histopathological and phylogenetic aspects. [So] Source: Vet Parasitol;228:144-152, 2016 Sep 15. [Is] ISSN: 1873-2550 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Nematode parasites of the cardiopulmonary system of livestock and pet animals have been receiving attention due to the pathogenic effects they produce in the course of the infection. However, parasitism in wild animals by metastrongilid nematodes has been neglected, resulting in potential risk to wildlife. Heterostrongylus heterostrongylus is the etiological agent of bronchial pneumonia in the black-eared opossum, Didelphis aurita, a widely distributed marsupial frequently reported to inhabit areas from wild environments to peri-urban spaces. In this study, we reassessed the taxonomy of H. heterostrongylus, describing and comparing morphology of L and L larvae and adult worms with closely related angiostrongilids, and inferring phylogenetic affinities within the family Angiostrongylidae; we also detailed histopathological reactions under natural infection. Ultrastructural morphology and light microscopy confirmed cephalic structures with well-developed trilobed lips, patterns of caudal bursa rays, spicules shape, terminal anal aperture and presence of the ventral protuberance in L larvae, characteristics present for the family Angiostrongylidae. Our molecular phylogenetic analyses, based on the partial small subunit (SSU) 18S rRNA gene, suggested that H. heterostrongylus and Didelphostrongylus hayesi are closely related. Pathological analyses agreed with previous findings, showing that cardiopulmonary parasitism provokes verminous pneumonia and mucous bronchiolitis with hypertrophy of the smooth bronchiolar muscle. These data confirmed that H. heterostrongylus represents a pulmonary pathogen in D. aurita, produces severe pathological effects, and may represent risk to domestic and wild animals. [Mh] Termos MeSH primário: Didelphis/parasitologia Metastrongyloidea/classificação Infecções por Strongylida/veterinária [Mh] Termos MeSH secundário: Animais DNA de Helmintos/química DNA de Helmintos/genética DNA Ribossômico/química DNA Ribossômico/genética Feminino Pulmão/parasitologia Pulmão/patologia Masculino Metastrongyloidea/genética Metastrongyloidea/isolamento & purificação Metastrongyloidea/ultraestrutura Microscopia Eletrônica de Varredura/veterinária Filogenia Análise de Sequência de DNA América do Sul Infecções por Strongylida/diagnóstico Infecções por Strongylida/parasitologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Helminth); 0 (DNA, Ribosomal) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170807 [Lr] Data última revisão: 170807 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161004 [St] Status: MEDLINE

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[PMID]: 27691942 [Au] Autor: Silva EM; Alves LC; Guerra NR; Farias MP; Oliveira EL; de Souza RC; da Cunha C; Ramos RA; Porto WJ [Ti] Título: LEISHMANIA SPP. IN DIDELPHIS SPP. FROM NORTHEASTERN BRAZIL. [So] Source: J Zoo Wildl Med;47(3):942-944, 2016 Sep. [Is] ISSN: 1042-7260 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The synanthropic behavior of marsupials of the genus Didelphis in endemic areas of leishmaniasis suggests that these animals may play an important role in the epidemiology of this infection. The aim of the present study was to detect Leishmania spp. DNA in Didelphis albiventris (white-eared opossum) and Didelphis aurita (big-eared opossum) living in forested and peridomestic areas of northeastern Brazil. Blood samples were collected from 25 animals (23 D. albiventris and 2 D. aurita ) by cardiac puncture and then analyzed via PCR. Leishmania (Viannia) braziliensis DNA complex was detected in 4 (16%) of the 25 samples, whereas no samples scored positive for Leishmania (Leishmania) donovani complex. Our data suggest that D. albiventris participates in the wild enzootic cycle of the L. (V.) braziliensis complex. Therefore, humans living in this area are at risk of infection, because Didelphis species are in constant movement between wild and urban environments. [Mh] Termos MeSH primário: Didelphis/parasitologia Leishmania/isolamento & purificação Leishmaniose/veterinária [Mh] Termos MeSH secundário: Animais Brasil/epidemiologia Leishmaniose/epidemiologia Leishmaniose/parasitologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170107 [Lr] Data última revisão: 170107 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161004 [St] Status: MEDLINE

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[PMID]: 27631688 [Au] Autor: Paiz LM; Donalisio MR; Richini-Pereira VB; Motoie G; Castagna CL; Tolezano JE [Ad] Endereço: 1 Department of Public Health, School of Medical Sciences, State University of Campinas-UNICAMP , Campinas, Brazil . [Ti] Título: Infection by Leishmania spp. in Free-Ranging Opossums (Didelphis albiventris) in an Environmentally Protected Area Inhabited by Humans in Southeastern Brazil. [So] Source: Vector Borne Zoonotic Dis;16(11):728-730, 2016 Nov. [Is] ISSN: 1557-7759 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: There is a growing concern about the participation of wild hosts and reservoirs in the epidemiology of leishmaniasis, particularly within the context of increasingly frequent environmental changes and the expansion of the One Health concept. This work is a molecular research of infection by Leishmania spp. among the wildlife of an environmentally protected area located in the municipality of Campinas, São Paulo, Brazil. The studied area has a history of intense environmental changes, with notifications of human cases of cutaneous leishmaniasis in the 1990s, and a focus of canine visceral leishmaniasis since 2009. Eighty-two wild mammals were sampled by monthly captures in this region over a 1-year period. Blood samples were collected from each animal and subjected to DNA extraction and PCR using primers for the region of the internal transcribed spacer-1. The results of gene sequencing for the first time revealed the infection of opossums (Didelphis albiventris) by Leishmania spp., subgenera Leishmania and Viannia, in Campinas. These findings, in addition to environmental and historical characteristics of the studied area, indicate a possible role of wildlife in the introduction and/or maintenance of natural foci of leishmaniasis transmission. [Mh] Termos MeSH primário: Didelphis Leishmaniose Visceral/veterinária [Mh] Termos MeSH secundário: Animais Animais Selvagens Brasil/epidemiologia Conservação dos Recursos Naturais Seres Humanos Leishmaniose Visceral/epidemiologia Leishmaniose Visceral/parasitologia Zoonoses [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170630 [Lr] Data última revisão: 170630 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160916 [St] Status: MEDLINE

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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