Base de dados : MEDLINE
Pesquisa : B01.050.150.900.649.709.235 [Categoria DeCS]
Referências encontradas : 1430 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 143 ir para página                         

  1 / 1430 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28337680
[Au] Autor:Jian R; Wang SW; Zhang WX; Zhang LP
[Ad] Endereço:Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, 050024, Hebei, People's Republic of China.
[Ti] Título:Morphological and molecular identification of Habronema spp. (Nematoda: Habronematidae) from donkeys in Xinjiang, China, and notes on the taxonomical status of Habronema majus (Creplin, 1849) and H. microstoma (Schneider, 1866).
[So] Source:Syst Parasitol;94(4):511-525, 2017 May.
[Is] ISSN:1573-5192
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Habronematid nematodes were collected from the stomachs of donkeys, Equus asinus L., in the Tarim Basin, Xinjiang, China. After examination by light and scanning electron microscopy, Habronema muscae (Carter, 1861) and H. majus (Creplin, 1849) were identified. The morphology of our specimens representing H. muscae (Carter, 1861) agreed well with previous redescriptions in the shape of the lateral lips, origin of the lateral alae, ratio of left and right spicules, and number and arrangement of caudal papillae. However, H. majus (Creplin, 1849) differs from H. microstoma (Schneider, 1866) in the arrangement of the caudal papillae in the male. Moreover, molecular analysis also showed interspecific differences of 26.2-28.2% in ITS2 and 8.6-8.9% in cox1 between H. majus and H. microstoma, a divergence much higher than the known intraspecific variation of Habronema spp. (6.6-8.7% in ITS2; 0.2-2.2% in cox1). The results indicate that both H. microstoma (Schneider, 1866) and H. majus (Creplin, 1849) are valid species.
[Mh] Termos MeSH primário: Equidae/parasitologia
Spiruroidea/classificação
[Mh] Termos MeSH secundário: Animais
China
DNA Espaçador Ribossômico/genética
Complexo IV da Cadeia de Transporte de Elétrons/genética
Feminino
Variação Genética
Masculino
Especificidade da Espécie
Spiruroidea/anatomia & histologia
Spiruroidea/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal Spacer); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170616
[Lr] Data última revisão:
170616
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1007/s11230-017-9714-8


  2 / 1430 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28422966
[Au] Autor:Bennett EA; Champlot S; Peters J; Arbuckle BS; Guimaraes S; Pruvost M; Bar-David S; Davis SJM; Gautier M; Kaczensky P; Kuehn R; Mashkour M; Morales-Muñiz A; Pucher E; Tournepiche JF; Uerpmann HP; Balasescu A; Germonpré M; Gündem CY; Hemami MR; Moullé PE; Ötzan A; Uerpmann M; Walzer C; Grange T; Geigl EM
[Ad] Endereço:Institut Jacques Monod, UMR7592, CNRS-Université Paris Diderot, Paris, France.
[Ti] Título:Taming the late Quaternary phylogeography of the Eurasiatic wild ass through ancient and modern DNA.
[So] Source:PLoS One;12(4):e0174216, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Taxonomic over-splitting of extinct or endangered taxa, due to an incomplete knowledge of both skeletal morphological variability and the geographical ranges of past populations, continues to confuse the link between isolated extant populations and their ancestors. This is particularly problematic with the genus Equus. To more reliably determine the evolution and phylogeographic history of the endangered Asiatic wild ass, we studied the genetic diversity and inter-relationships of both extinct and extant populations over the last 100,000 years, including samples throughout its previous range from Western Europe to Southwest and East Asia. Using 229 bp of the mitochondrial hypervariable region, an approach which allowed the inclusion of information from extremely poorly preserved ancient samples, we classify all non-African wild asses into eleven clades that show a clear phylogeographic structure revealing their phylogenetic history. This study places the extinct European wild ass, E. hydruntinus, the phylogeny of which has been debated since the end of the 19th century, into its phylogenetic context within the Asiatic wild asses and reveals recent mitochondrial introgression between populations currently regarded as separate species. The phylogeographic organization of clades resulting from these efforts can be used not only to improve future taxonomic determination of a poorly characterized group of equids, but also to identify historic ranges, interbreeding events between various populations, and the impact of ancient climatic changes. In addition, appropriately placing extant relict populations into a broader phylogeographic and genetic context can better inform ongoing conservation strategies for this highly-endangered species.
[Mh] Termos MeSH primário: Conservação de Recursos Naturais
DNA Mitocondrial/genética
Espécies em Perigo de Extinção
Equidae/genética
Filogenia
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Equidae/anatomia & histologia
Equidae/classificação
Europa (Continente)
Extinção Biológica
Extremo Oriente
Fósseis
Variação Genética
Haplotipos
Filogeografia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174216


  3 / 1430 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27667749
[Au] Autor:Carluccio A; Contri A; Gloria A; Veronesi MC; Sfirro MP; Parrillo S; Robbe D
[Ad] Endereço:Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
[Ti] Título:Correlation between some arterial and venous blood gas parameters in healthy newborn Martina Franca donkey foals from birth to 96 hours of age.
[So] Source:Theriogenology;87:173-178, 2017 Jan 01.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In neonatology, blood gas analysis is a useful tool in the evaluation of the health of newborns and plays a key role in early detection of critically ill subjects. Because blood gas analysis parameters have not previously been studied in any depth in donkey foals, this study was performed on 16 healthy Martina Franca donkey foals born after an uncomplicated delivery. Arterial and venous blood samples were collected at 5 minutes and at 12, 24, 72, and 96 hours of age. Blood gas analysis was performed by a portable analyzer, measuring arterial and venous total carbon dioxide, carbon dioxide partial pressure (pCO ), oxygen partial pressure (pO ), oxygen saturation (sO ), bicarbonate, base excess (BE), pH, and lactate (LT). Lower blood pH values, pO and sO , and a higher level of lactate were found at birth in comparison with subsequent sampling times. This moderate acidotic profile disappeared at 12 hours, when all the parameters became constant until the end of the study period. As expected, significant differences between arterial and venous blood gas parameters related to the oxygenation, such as pO and sO , and partially carbon dioxide partial pressure were found, whereas total carbon dioxide, pH, BE, and LT were comparable in arterial and venous blood samples. For these latter parameters, the highly significant correlation between arterial and venous findings suggests that venous samples could be an acceptable alternative to the arterial sample for blood gas analysis in newborn donkey foals, when the oxygenation status of the patient is not the first goal of patient analysis.
[Mh] Termos MeSH primário: Animais Recém-Nascidos/sangue
Dióxido de Carbono/sangue
Equidae/sangue
Oxigênio/sangue
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos/fisiologia
Bicarbonatos/sangue
Equidae/fisiologia
Concentração de Íons de Hidrogênio
Lactatos/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bicarbonates); 0 (Lactates); 142M471B3J (Carbon Dioxide); S88TT14065 (Oxygen)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160926
[St] Status:MEDLINE


  4 / 1430 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28233669
[Au] Autor:Rudramurthy GR; Sengupta PP; Ligi M; Rahman H
[Ad] Endereço:National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Ramagondanahalli, P.B.No. 6450, Yelahanka, Bengaluru 560064, Karnataka, India.
[Ti] Título:An inhibition enzyme immuno assay exploring recombinant invariant surface glycoprotein and monoclonal antibodies for surveillance of surra in animals.
[So] Source:Biologicals;46:148-152, 2017 Mar.
[Is] ISSN:1095-8320
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study is aimed at the development of inhibition ELISA (I-ELISA) exploring monoclonal antibodies (MAbs) and recombinant invariant surface glycoprotein. The extracellular domain (ED) of invariant surface glycoprotein (ISG-75) from Trypanosoma evasni has been heterologously expressed in Pichia pastoris (X-33). The recombinant ISG-75 (rISG-75ED) was characterized by immunoblot and ELISA, followed by the production of MAbs against rISG-75ED. The MAbs were characterized by immunoblot and then explored in the development of I-ELISA for the detection of surra. The diagnostic potential of the developed test has been evaluated using 1192 field sera sample including cattle, buffalo, donkey, horse and camel. The statistical analysis of the data showed optimum combination of diagnostic sensitivity and specificity at 98.8% and 99.2% respectively, with cut-off percentage inhibition (PI) value of >45. The Cohen's kappa coefficient of agreement was found to be 0.98. Hence, the diagnostic test developed in the present study can be exploited as a potential and reliable tool in the serodiagnosis and surveillance of surra in animals.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/imunologia
Glicoproteínas de Membrana/imunologia
Proteínas de Protozoários/imunologia
Proteínas Recombinantes/imunologia
Trypanosoma/imunologia
Tripanossomíase/imunologia
[Mh] Termos MeSH secundário: Animais
Búfalos
Camelus
Bovinos
Ensaio de Imunoadsorção Enzimática/métodos
Equidae
Cavalos
Interações Hospedeiro-Parasita/imunologia
Immunoblotting
Glicoproteínas de Membrana/genética
Proteínas de Protozoários/genética
Sensibilidade e Especificidade
Trypanosoma/fisiologia
Tripanossomíase/diagnóstico
Tripanossomíase/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Membrane Glycoproteins); 0 (Protozoan Proteins); 0 (Recombinant Proteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170418
[Lr] Data última revisão:
170418
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE


  5 / 1430 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28260587
[Au] Autor:Carvalho I; Campo RD; Sousa M; Silva N; Carrola J; Marinho C; Santos T; Carvalho S; Nóvoa M; Quaresma M; Pereira JE; Cobo M; Igrejas G; Poeta P
[Ad] Endereço:1​Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal 2​Department of Genetics and Biotechnology, Vila Real, Portugal 3​Functional Genomics and Proteomics Unit, Vila Real, Portugal.
[Ti] Título:Antimicrobial-resistant Escherichia coli and Enterococcus spp. isolated from Miranda donkey (Equus asinus): an old problem from a new source with a different approach.
[So] Source:J Med Microbiol;66(2):191-202, 2017 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The Miranda donkey (Equus asinus) is an endangeredasinine from Miranda do Douro region, located in the north east of Portugal. We studied the antimicrobial resistance and virulence genes in Escherichia coli and Enterococcus spp. isolates from these animals. METHODOLOGY: In March 2014, a total of 66 faecal samples were recovered from independent animals. Antibiotic resistance was determined by the disc diffusion method. Carriage of genes coding for antibiotic-resistant and virulent factors was analysed by PCR. RESULTS: A total of 66 E. coli and 41 enterococcal isolates were detected, with Enterococcus faecium (61 %) and Enterococcus hirae (24 %) being the most prevalent species. For enterococcal isolates, high percentages of resistance rates to tetracycline (68.3 %), quinupristin/dalfopristin (51.2 %) and ciprofloxacin (48.8 %) were observed. The genes erm(A) and/or erm(B), tet(M) and/or tet(L), vat(D) and/or vat(E) and aph(3')-IIIa were also found. The most frequent virulence gene detected was gel(E), followed by ace, cpd and hyl. Escherichia coli isolates were highly resistant to streptomycin (78 %), whereas 39 % of them exhibited resistance to aminoglycosides and tetracycline. Genes sul1 and/or sul2 were detected in 66.7 % of trimethoprim/sulfamethoxazole-resistant isolates. The virulence genes detected were fim(A) (46 %) and cnf1 (27 %). CONCLUSION: To the best of our knowledge, this is the first report showing antibiotic resistance among Escherichiacoli and Enterococcus spp. isolates from the Miranda donkey in Portugal, indicating possible antibiotic-resistant bacterial reservoirs. However, the detection of these resistances presents a low risk for other animals and human beings in that rural area.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana Múltipla
Enterococcus/genética
Equidae/microbiologia
Escherichia coli/genética
[Mh] Termos MeSH secundário: Aminoglicosídeos/farmacologia
Animais
Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Ciprofloxacino/farmacologia
DNA Bacteriano/genética
Enterococcus/classificação
Enterococcus/isolamento & purificação
Escherichia coli/classificação
Escherichia coli/isolamento & purificação
Canamicina Quinase/genética
Metiltransferases/genética
Testes de Sensibilidade Microbiana
Portugal
Sulfametoxazol/farmacologia
Tetraciclina/farmacologia
Trimetoprima/farmacologia
Virginiamicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aminoglycosides); 0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 11006-76-1 (Virginiamycin); 126602-89-9 (quinupristin-dalfopristin); 5E8K9I0O4U (Ciprofloxacin); AN164J8Y0X (Trimethoprim); EC 2.1.1.- (Methyltransferases); EC 2.1.1.184 (ErmA protein, Bacteria); EC 2.7.1.95 (Kanamycin Kinase); F8VB5M810T (Tetracycline); JE42381TNV (Sulfamethoxazole)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000423


  6 / 1430 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28281774
[Au] Autor:Horak IG; Heyne H; Halajian A; Booysen S; Smit WJ
[Ad] Endereço:Department of Veterinary Tropical Diseases, University of Pretoria. ivan.horak@up.ac.za.
[Ti] Título:Parasites of domestic and wild animals in South Africa. L. Ixodid ticks infesting horses and donkeys.
[So] Source:Onderstepoort J Vet Res;84(1):e1-e6, 2017 Feb 28.
[Is] ISSN:2219-0635
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:The aim of the study was to determine the species spectrum of ixodid ticks that infest horses and donkeys in South Africa and to identify those species that act as vectors of disease to domestic livestock. Ticks were collected opportunistically from 391 horses countrywide by their owners or grooms, or by veterinary students and staff at the Faculty of Veterinary Science, University of Pretoria. Ticks were also collected from 76 donkeys in Limpopo Province, 2 in Gauteng Province and 1 in North West province. All the ticks were identified by means of a stereoscopic microscope. Horses were infested with 17 tick species, 72.1% with Rhipicephalus evertsi evertsi, 19.4% with Amblyomma hebraeum and 15.6% with Rhipicephalus decoloratus. Rhipicephalus evertsi evertsi was recovered from horses in all nine provinces of South Africa and R. decoloratus in eight provinces. Donkeys were infested with eight tick species, and 81.6% were infested with R. evertsi evertsi, 23.7% with A. hebraeum and 10.5% with R. decoloratus. Several tick species collected from the horses and donkeys are the vectors of economically important diseases of livestock. Rhipicephalus evertsi evertsi is the vector of Theileria equi, the causative organism of equine piroplasmosis. It also transmits Anaplasma marginale, the causative organism of anaplasmosis in cattle. Amblyomma hebraeum is the vector of Ehrlichia ruminantium, the causative organism of heartwater in cattle, sheep and goats, whereas R. decoloratus transmits Babesia bigemina, the causative organism of babesiosis in cattle.
[Mh] Termos MeSH primário: Vetores Aracnídeos
Equidae/parasitologia
Doenças dos Cavalos/epidemiologia
Ixodidae
Infestações por Carrapato/veterinária
Doenças Transmitidas por Carrapatos/veterinária
[Mh] Termos MeSH secundário: Animais
Animais Selvagens
Doenças dos Cavalos/parasitologia
Doenças dos Cavalos/transmissão
Cavalos
Prevalência
África do Sul/epidemiologia
Infestações por Carrapato/epidemiologia
Infestações por Carrapato/parasitologia
Doenças Transmitidas por Carrapatos/epidemiologia
Doenças Transmitidas por Carrapatos/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.4102/ojvr.v84i1.1302


  7 / 1430 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28252356
[Au] Autor:Muhatai G; Cheng L; Rugoho I; Xiao G; Chen G; Hodge S; Zhou X
[Ad] Endereço:College of Animal Science,Tarim University,Alaer,Xinjiang,843300,PR China.
[Ti] Título:Effect of parity, milking time and stage of lactation on milk yield of Jiangyue donkey (Equus asinus) in North West China.
[So] Source:J Dairy Res;84(1):23-26, 2017 Feb.
[Is] ISSN:1469-7629
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The study reported in this Research Communication was carried out to examine how parity and milking time affected donkey milk yield under a typical Chinese production system. Eighteen Jiangyue breed donkeys with good health condition, aged between 6 and 9 years, and with parities 3 and 4, were selected. Milk production was measured commencing from the fourth week post-foaling, with a total data collection of 170 d in milk (lactation length). Mean milk yield of the donkeys used in this study was 3·0 kg/donkey/d. Results showed milk yield decreased with days in milk from 3·3 kg/donkey/d at the start of the study, to 2·2 kg/donkey/d by the end of the 170 d. Parity 3 donkeys produced 22% more milk than parity 4 donkeys (3·3 kg/donkey/d vs 2·7 kg/donkey/d). The information provided by this study should aid producers in estimating the expected milk yields from individual donkey, and better predict milk yield over the course of a production cycle.
[Mh] Termos MeSH primário: Indústria de Laticínios/métodos
Equidae/fisiologia
Lactação/fisiologia
Paridade/fisiologia
[Mh] Termos MeSH secundário: Animais
China
Dieta/veterinária
Feminino
Leite
Gravidez
Estações do Ano
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170316
[Lr] Data última revisão:
170316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE
[do] DOI:10.1017/S0022029916000777


  8 / 1430 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28080902
[Au] Autor:Lopez KM; Fleming GJ; Mylniczenko ND
[Ti] Título:A SEROLOGIC AND POLYMERASE CHAIN REACTION SURVEY OF EQUINE HERPESVIRUS IN BURCHELL'S ZEBRAS (EQUUS QUAGGA), HARTMANN'S MOUNTAIN ZEBRAS (EQUUS ZEBRA HARTMANNAE), AND THOMSON'S GAZELLES (EUDORCAS THOMSONII) IN A MIXED SPECIES SAVANNAH EXHIBIT.
[So] Source:J Zoo Wildl Med;47(4):1013-1018, 2016 Dec.
[Is] ISSN:1042-7260
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reports of equine herpesvirus (EHV) 1 and EHV-9 causing clinical disease in a wide range of species have been well documented in the literature. It is thought that zebras are the natural hosts of EHV-9 both in the wild and in captive collections. Concerns about potential interspecies transmission of EHV-1 and EHV-9 in a mixed species savannah exhibit prompted serologic and polymerase chain reaction surveys. Eighteen Burchell's zebras ( Equus quagga ), 11 Hartmann's mountain zebras ( Equus zebra hartmannae), and 14 Thomson's gazelles ( Eudorcas thomsonii ) cohabitating the same exhibit were examined for EHV-1 virus neutralization titers, and evidence of virus via EHV 1-5 polymerase chain reactions. None of the animals had previous exposure to vaccination with EHV-1 or EHV-4. All tested zebras had positive EHV-1 titers, ranging from 4 to 384. All zebras and Thomson's gazelles had negative polymerase chain reaction results for all targeted equine herpesviruses. EHV-9-specific assays are not available but EHV-1, EHV-4, and EHV-9 cross-react serologically. Positive serology results indicate a potential latent equine herpesvirus in the zebra population, which prompted initiation of an equine herpesvirus vaccine protocol, changes in pregnant zebra mare management, and equine herpesvirus polymerase chain reaction screening prior to shipment to or from the study site.
[Mh] Termos MeSH primário: Antílopes
Equidae
Herpesvirus Equídeo 1/isolamento & purificação
Herpesvirus Equídeo 4/isolamento & purificação
Reação em Cadeia da Polimerase/veterinária
Testes Sorológicos/veterinária
[Mh] Termos MeSH secundário: Animais
Animais de Zoológico
Antílopes/virologia
Equidae/virologia
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE
[do] DOI:10.1638/2013-0297.1


  9 / 1430 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27979136
[Au] Autor:Ummarino S; Mozzon M; Zamporlini F; Amici A; Mazzola F; Orsomando G; Ruggieri S; Raffaelli N
[Ad] Endereço:Department of Agricultural, Food and Environmental Sciences, Università Politecnica delle Marche, Via Brecce Bianche, Ancona 60131, Italy. Electronic address: s.ummarino@univpm.it.
[Ti] Título:Simultaneous quantitation of nicotinamide riboside, nicotinamide mononucleotide and nicotinamide adenine dinucleotide in milk by a novel enzyme-coupled assay.
[So] Source:Food Chem;221:161-168, 2017 Apr 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nicotinamide riboside, the most recently discovered form of vitamin B3, and its phosphorylated form nicotinamide mononucleotide, have been shown to be potent supplements boosting intracellular nicotinamide adenine dinucleotide (NAD) levels, thus preventing or ameliorating metabolic and mitochondrial diseases in mouse models. Here we report for the first time on the simultaneous quantitation of nicotinamide riboside, nicotinamide mononucleotide and NAD in milk by means of a fluorometric, enzyme-coupled assay. Application of this assay to milk from different species revealed that the three vitamers were present in human and donkey milk, while being selectively distributed in the other milks. Human milk was the richest source of nicotinamide mononucleotide. Overall, the three vitamers accounted for a significant fraction of total vitamin B3 content. Pasteurization did not affect the bovine milk content of nicotinamide riboside, whereas UHT processing fully destroyed the vitamin. In human milk, NAD levels were significantly affected by the lactation time.
[Mh] Termos MeSH primário: Ensaios Enzimáticos/métodos
Análise de Alimentos
Leite/química
NAD/análise
Niacinamida/análogos & derivados
Mononucleotídeo de Nicotinamida/análise
[Mh] Termos MeSH secundário: Animais
Bovinos
Equidae
Fluorometria
Manipulação de Alimentos
Humanos
Leite Humano/química
Niacinamida/análise
Pasteurização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0U46U6E8UK (NAD); 1094-61-7 (Nicotinamide Mononucleotide); 1341-23-7 (nicotinamide-beta-riboside); 25X51I8RD4 (Niacinamide)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE


  10 / 1430 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28155284
[Au] Autor:Ndeereh D; Thaiyah A; Muchemi G; Miyunga AA
[Ad] Endereço:Department of Veterinary Services, Kenya Wildlife Service. dndeereh@kws.go.ke.
[Ti] Título:Molecular surveillance of spotted fever group rickettsioses in wildlife and detection of Rickettsia sibirica in a Topi (Damaliscus lunatus ssp. jimela) in Kenya.
[So] Source:Onderstepoort J Vet Res;84(1):e1-e7, 2017 Jan 30.
[Is] ISSN:2219-0635
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:Spotted fever group rickettsioses are a group of tick-borne zoonotic diseases caused by intracellular bacteria of the genus Rickettsia. The diseases are widely reported amongst international travellers returning from most sub-Saharan Africa with fever, yet their importance in local populations largely remains unknown. Although this has started to change and recently there have been increasing reports of the diseases in livestock, ticks and humans in Kenya, they have not been investigated in wildlife. We examined the presence, prevalence and species of Rickettsia present in wildlife in two regions of Kenya with a unique human-wildlife-livestock interface. For this purpose, 79 wild animals in Laikipia County and 73 in Maasai Mara National Reserve were sampled. DNA extracted from blood was tested using the polymerase chain reaction (PCR) to amplify the intergenic spacer rpmE-tRNAfMet and the citrate synthase-encoding gene gltA. Rickettsial DNA was detected in 2 of the 79 (2.5%) animals in Laikipia and 4 of the 73 (5.5%) in Maasai Mara. The PCR-positive amplicons of the gltA gene were sequenced to determine the detected Rickettsia species. This revealed Rickettsia sibirica in a Topi (Damaliscus lunatus ssp. jimela). This is the first report of spotted fever group rickettsioses in wildlife and the first to report R. sibirica in Kenya. The finding demonstrates the potential role of wild animals in the circulation of the diseases.
[Mh] Termos MeSH primário: Animais Selvagens/microbiologia
Antílopes/microbiologia
Infecções por Rickettsia/veterinária
Rickettsia
[Mh] Termos MeSH secundário: Animais
Búfalos/microbiologia
DNA Bacteriano/genética
Equidae/microbiologia
Humanos
Quênia/epidemiologia
Reação em Cadeia da Polimerase/veterinária
Vigilância da População
Prevalência
Rickettsia/genética
Infecções por Rickettsia/epidemiologia
Infecções por Rickettsia/transmissão
Zoonoses/epidemiologia
Zoonoses/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.4102/ojvr.v84i1.1265



página 1 de 143 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde