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[PMID]:28401328
[Au] Autor:Worku Y; Wondimagegn W; Aklilu N; Assefa Z; Gizachew A
[Ad] Endereço:Livestock and Fishers Office of Oromia Region, Oromia, Ethiopia.
[Ti] Título:Equine colic: clinical epidemiology and associated risk factors in and around Debre Zeit.
[So] Source:Trop Anim Health Prod;49(5):959-965, 2017 Jun.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A prospective study was conducted to describe clinical epidemiology of equine colic in the Society for Protection of Animal Abroad and Donkey Sanctuary Project Clinic, at Debre Zeit, Ethiopia, from November 2014 to April 2015. The objectives were to describe clinical epidemiology of equine colic, to characterize the main types of equine colic, and to determine the major risk factors associated with equine colic. The method which was used in the study was attending clinical case of equine and assessing physiological parameters, fecal egg count, abdominal sounds, and rectal examination as well as questioner interviewing of the owners. The data were collected and analyzed using Statistical Package for Social Science. The incidence of colic was 10.3% in the study period. Colicky were typed as unknown, flatulent, impaction, spasmodic, and enterolithiasis. The proportion of case incidence were 63.1 (41/65), 33.8 (22/65), and 3.1% (2/65), in donkey, horse, and mule, respectively. The total mean (±SD) of temperature 37.80 ± 1.003, heart rate 57.54 ± 10.098, fecal egg count 236.922 ± 67.990, respiratory rate 30.92 ± 7.315, and packed cell volume 41.40 ± 10.221 were recorded. The case fatality rate of equine colic was 15.38% (10/65). There were statistically highly significant (p < 0.01) differences in impaction colic in relation to species. Interview with 183 equine owners revealed incidence of equine colic as the sixth major disease condition affecting equine. A long-term epidemiological study of the true representative population should be carried out to determine the incidence rate and associated risk factors of equine colic in the study area.
[Mh] Termos MeSH primário: Cólica/veterinária
Equidae
Doenças dos Cavalos/epidemiologia
[Mh] Termos MeSH secundário: Animais
Cólica/classificação
Cólica/epidemiologia
Cólica/etiologia
Etiópia/epidemiologia
Feminino
Doenças dos Cavalos/classificação
Doenças dos Cavalos/etiologia
Cavalos
Incidência
Masculino
Estudos Prospectivos
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-017-1283-y


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[PMID]:28536880
[Au] Autor:Dibaba MD; Getachew AM; Assefa Z; Fanta A; Etana M; Firew S; Goshu L; Burden F
[Ad] Endereço:College of Veterinary Medicine and Agriculture, Addis Ababa University, Addis Ababa, Ethiopia.
[Ti] Título:Seasonal variation of strongylosis in working donkeys of Ethiopia: a cross-sectional and longitudinal studies.
[So] Source:Parasitol Res;116(7):2009-2015, 2017 Jul.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Helminths are one of the major health problems of working donkeys, often with heavy worm burden and contributing to their early demise and/or reduction in their work output. Cross-sectional and longitudinal studies were conducted to investigate the current infection prevalence and level of strongyles infection donkeys would acquire through different seasons in the mid-lowland agro-ecological zones of Ethiopia. For this purpose, faecal samples from 206 (cross-sectional study) and 102 (longitudinal study) randomly selected donkeys were directly collected from the rectum and analysed. For the longitudinal study, the 102 donkeys dewormed at the end of main rainy season, beginning of October, were monitored for the level of strongyle infection they would acquire during subsequent dry and short rainy seasons. The cross-sectional study of 206 donkey has revealed an overall infection prevalence of 89.3% (95% confidence interval (CI) = 84.4, 92.9). Donkeys in the lowland zone showed a significantly higher strongyle infection prevalence (P = 0.0126) and mean eggs per gramme of faces (EPG) (P = 0.001; 2775 EPG) compared to donkeys in the midland zone (980.8 EPG). Age, sex and body condition did not have any significant effect on either the infection prevalence or level of infection (P > 0.05). The longitudinal study has shown a significantly lower strongyle infection prevalence (P = 0.003) and level of infection donkeys acquired (P = 0.001) in the subsequent dry and short rainy seasons compared to the main rainy season following October deworming. However, these values were not significantly different between the two agro-ecological zones (P > 0.05). This study clearly showed that parasitic infections are primarily acquired during the main rainy season when pasture/herbage coverage is relatively better, and the environment is conducive for parasites survival and development. On the other hand, the finding of majority of donkeys shedding low or no eggs during the dry and short rainy seasons showed that October deworming was effective, and donkeys acquire low or no parasitic infection during the subsequent dry and short rainy seasons. Therefore, the practice of anthelmintic treatment of donkeys at the end of short rain in May may not be necessary, and October deworming once a year is sufficient.
[Mh] Termos MeSH primário: Equidae
Infecções por Strongylida/veterinária
Estrongilídios
[Mh] Termos MeSH secundário: Animais
Estudos Transversais
Etiópia/epidemiologia
Fezes/parasitologia
Feminino
Estudos Longitudinais
Masculino
Prevalência
Chuvas
Estações do Ano
Infecções por Strongylida/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-017-5485-z


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[PMID]:28337680
[Au] Autor:Jian R; Wang SW; Zhang WX; Zhang LP
[Ad] Endereço:Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, 050024, Hebei, People's Republic of China.
[Ti] Título:Morphological and molecular identification of Habronema spp. (Nematoda: Habronematidae) from donkeys in Xinjiang, China, and notes on the taxonomical status of Habronema majus (Creplin, 1849) and H. microstoma (Schneider, 1866).
[So] Source:Syst Parasitol;94(4):511-525, 2017 May.
[Is] ISSN:1573-5192
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Habronematid nematodes were collected from the stomachs of donkeys, Equus asinus L., in the Tarim Basin, Xinjiang, China. After examination by light and scanning electron microscopy, Habronema muscae (Carter, 1861) and H. majus (Creplin, 1849) were identified. The morphology of our specimens representing H. muscae (Carter, 1861) agreed well with previous redescriptions in the shape of the lateral lips, origin of the lateral alae, ratio of left and right spicules, and number and arrangement of caudal papillae. However, H. majus (Creplin, 1849) differs from H. microstoma (Schneider, 1866) in the arrangement of the caudal papillae in the male. Moreover, molecular analysis also showed interspecific differences of 26.2-28.2% in ITS2 and 8.6-8.9% in cox1 between H. majus and H. microstoma, a divergence much higher than the known intraspecific variation of Habronema spp. (6.6-8.7% in ITS2; 0.2-2.2% in cox1). The results indicate that both H. microstoma (Schneider, 1866) and H. majus (Creplin, 1849) are valid species.
[Mh] Termos MeSH primário: Equidae/parasitologia
Spiruroidea/classificação
[Mh] Termos MeSH secundário: Animais
China
DNA Espaçador Ribossômico/genética
Complexo IV da Cadeia de Transporte de Elétrons/genética
Feminino
Variação Genética
Masculino
Especificidade da Espécie
Spiruroidea/anatomia & histologia
Spiruroidea/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal Spacer); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1007/s11230-017-9714-8


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[PMID]:28126600
[Au] Autor:Lizarraga I; Castillo-Alcala F; Robinson LS
[Ad] Endereço:Department of Biomedical Sciences, Ross University School of Veterinary Medicine, P.O. Box 334, Basseterre, Saint Kitts and Nevis. Electronic address: madrigal@unam.mx.
[Ti] Título:Sedative and mechanical antinociceptive effects of four dosages of romifidine administered intravenously to donkeys.
[So] Source:Res Vet Sci;112:46-51, 2017 Jun.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although romifidine is commonly used to provide sedation and analgesia for the facilitation of clinical procedures in donkeys, limited scientific information is available for this drug in this species. This randomized, controlled, crossover, Latin-square, blinded study compared the sedative and antinociceptive effects of four dosages of romifidine (40, 60, 80, and 100µg/kg IV; R40, R60, R80, and R100, respectively), acepromazine (0.1mg/kg IV; ACE) and saline (0.9%, 5mL IV) by assigning sedation scores (SS) and measuring head heights above ground (HHAG) and mechanical nociceptive thresholds (MNT) in donkeys. Areas under the curve (AUC) from 0 to 30, 30-60, 60-120, and 120-180min after administration were computed for SS, HHAG, and MNT and compared among treatments. Romifidine and ACE, but not saline, induced clinical signs of sedation. SS-AUC for R60, R80 and R100, and SS-AUC for R100 were higher than corresponding values for saline. HHAG-AUC for R40 and R80, and HHAG-AUC for R40, R60, R80 and R100 were smaller than for saline. HHAG-AUC for R100 were also smaller than those for ACE. Romifidine, but not saline or ACE, increased MNT. MNT-AUC and MNT-AUC for R40, R60, R80 and R100, and MNT-AUC for R80 and R100 were higher than corresponding values for saline and ACE. MNT-AUC for R100 were higher than for all other romifidine treatments. In donkeys, the degree of sedation was similar for the four dosages of romifidine, but antinociception was dose-dependent.
[Mh] Termos MeSH primário: Equidae
Imidazóis/farmacologia
Nociceptividade/efeitos de drogas
Dor/veterinária
[Mh] Termos MeSH secundário: Acepromazina/administração & dosagem
Acepromazina/farmacologia
Administração Intravenosa
Analgésicos/farmacologia
Anestésicos/administração & dosagem
Anestésicos/farmacologia
Animais
Antipsicóticos/administração & dosagem
Antipsicóticos/farmacologia
Área Sob a Curva
Sedação Consciente/veterinária
Estudos Cross-Over
Relação Dose-Resposta a Droga
Quimioterapia Combinada
Feminino
Hipnóticos e Sedativos
Imidazóis/administração & dosagem
Masculino
Dor/prevenção & controle
[Pt] Tipo de publicação:CONTROLLED CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Anesthetics); 0 (Antipsychotic Agents); 0 (Hypnotics and Sedatives); 0 (Imidazoles); 54EJ303F0R (Acepromazine); 876351L05K (romifidine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE


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[PMID]:28599175
[Au] Autor:Turchi B; Pedonese F; Torracca B; Fratini F; Mancini S; Galiero A; Montalbano B; Cerri D; Nuvoloni R
[Ad] Endereço:Department of Veterinary Science, Viale delle Piagge 2, University of Pisa, Italy. Electronic address: barbara.turchi@unipi.it.
[Ti] Título:Lactobacillus plantarum and Streptococcus thermophilus as starter cultures for a donkey milk fermented beverage.
[So] Source:Int J Food Microbiol;256:54-61, 2017 Sep 01.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Donkey milk is recently gaining attention due to its nutraceutical properties. Its low casein content does not allow caseification, so the production of a fermented milk would represent an alternative way to increase donkey milk shelf life. The aim of this study was to investigate the possibility of employing selected Streptococcus thermophilus and Lactobacillus plantarum isolates for the production of a novel donkey milk fermented beverage. Lysozyme resistance and the ability to acidify donkey milk were chosen as main selection parameters. Different fermented beverages (C1-C9) were produced, each with a specific combination of isolates, and stored at refrigerated conditions for 35days. The pH values and viability of the isolates were weekly assessed. In addition, sensory analysis was performed. Both S. thermophilus and L.plantarum showed a high degree of resistance to lysozyme with a Minimum Bactericidal Concentration>6.4mg/mL for 100% of S. thermophilus and 96% of L. plantarum. S. thermophilus and L. plantarum showed the ability to acidify donkey milk in 24h at 37°C, with an average ΔpH value of 2.91±0.16 and 1.78±0.66, respectively. Four L. plantarum and two S. thermophilus were chosen for the production of fermented milks. Those containing the association S. thermophilus/L. plantarum (C1-C4) reached a pH lower than 4.5 after 18h of fermentation and showed microbial loads higher than 7.00logcfu/mL until the end of the storage period. Moreover, comparing the microbial loads of samples containing both species and those containing S. thermophilus alone (C5), we highlighted the ability of L. plantarum to stimulate S. thermophilus replication. This boosted replication of S. thermophilus allowed to reach an appropriate pH in a time frame fitting the production schedule. This was not observed for samples containing a single species (C5-C9). Thus, L. plantarum strains seem to be good candidates in the production of a novel type of fermented milk, not only for their probiotic potential, but also for the enhancing effect on S. thermophilus growth.
[Mh] Termos MeSH primário: Produtos Fermentados do Leite/microbiologia
Lactobacillus plantarum/metabolismo
Leite/metabolismo
Muramidase/metabolismo
Streptococcus thermophilus/metabolismo
[Mh] Termos MeSH secundário: Animais
Bebidas/microbiologia
Reatores Biológicos
Equidae
Fermentação
Lactobacillus plantarum/isolamento & purificação
Leite/química
Probióticos/metabolismo
Streptococcus thermophilus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.2.1.17 (Muramidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE


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[PMID]:28901631
[Au] Autor:Seeber PA; Soilemetzidou SE; East ML; Walzer C; Greenwood AD
[Ad] Endereço:Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.
[Ti] Título:Equine behavioral enrichment toys as tools for non-invasive recovery of viral and host DNA.
[So] Source:Zoo Biol;36(5):341-344, 2017 Sep.
[Is] ISSN:1098-2361
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Direct collection of samples from wildlife can be difficult and sometimes impossible. Non-invasive remote sampling for the purpose of DNA extraction is a potential tool for monitoring the presence of wildlife at the individual level, and for identifying the pathogens shed by wildlife. Equine herpesviruses (EHV) are common pathogens of equids that can be fatal if transmitted to other mammals. Transmission usually occurs by nasal aerosol discharge from virus-shedding individuals. The aim of this study was to validate a simple, non-invasive method to track EHV shedding in zebras and to establish an efficient protocol for genotyping individual zebras from environmental DNA (eDNA). A commercially available horse enrichment toy was deployed in captive Grévy's, mountain, and plains zebra enclosures and swabbed after 4-24 hr. Using eDNA extracted from these swabs four EHV strains (EHV-1, EHV-7, wild ass herpesvirus and zebra herpesvirus) were detected by PCR and confirmed by sequencing, and 12 of 16 zebras present in the enclosures were identified as having interacted with the enrichment toy by mitochondrial DNA amplification and sequencing. We conclude that, when direct sampling is difficult or prohibited, non-invasive sampling of eDNA can be a useful tool to determine the genetics of individuals or populations and for detecting pathogen shedding in captive wildlife.
[Mh] Termos MeSH primário: Criação de Animais Domésticos/instrumentação
DNA Viral/isolamento & purificação
Equidae/virologia
Herpesviridae/genética
Jogos e Brinquedos
[Mh] Termos MeSH secundário: Animais
Comportamento Animal
Feminino
Herpesviridae/classificação
Herpesviridae/isolamento & purificação
Masculino
Eliminação de Partículas Virais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1002/zoo.21380


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[PMID]:28698676
[Au] Autor:Ma J; Lei H; Chen X; Bi X; Jiang Y; Han L; Huang X
[Ad] Endereço:Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang, PR China.
[Ti] Título:New anti-inflammatory metabolites produced by Streptomyces violaceoruber isolated from Equus burchelli feces.
[So] Source:J Antibiot (Tokyo);70(10):991-994, 2017 Oct.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Three new metabolites (2-4), together with one known compound, GTRI-02, (1) were isolated from a fermentation broth of Streptomyces violaceoruber derived from Equus burchelli feces. The structures of the new compounds 2-4 were established using comprehensive NMR spectroscopic data analysis as well as UV, IR and MS data. The anti-inflammatory activity of compounds 1-4 was tested by examining their ability to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Compound 2 showed a moderate inhibition of NO production with IC value of 51.2 µm.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Produtos Biológicos/farmacologia
Meios de Cultura/química
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/metabolismo
Animais
Anti-Inflamatórios/química
Anti-Inflamatórios/isolamento & purificação
Produtos Biológicos/química
Produtos Biológicos/isolamento & purificação
Equidae/microbiologia
Fezes/microbiologia
Concentração Inibidora 50
Lipopolissacarídeos/metabolismo
Macrófagos/efeitos de drogas
Macrófagos/metabolismo
Espectroscopia de Ressonância Magnética
Espectrometria de Massas
Camundongos
Óxido Nítrico/antagonistas & inibidores
Células RAW 264.7
Espectrofotometria Infravermelho
Espectrofotometria Ultravioleta
Streptomyces/crescimento & desenvolvimento
Streptomyces/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Anti-Inflammatory Agents); 0 (Biological Products); 0 (Culture Media); 0 (Lipopolysaccharides); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.1038/ja.2017.75


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[PMID]:28670118
[Au] Autor:Li X; Shi F; Gong L; Hang B; Li D; Chi L
[Ad] Endereço:National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, and State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, People's Republic of China.
[Ti] Título:Species-specific identification of collagen components in Colla corii asini using a nano-liquid chromatography tandem mass spectrometry proteomics approach.
[So] Source:Int J Nanomedicine;12:4443-4454, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Colla corii asini (CCA) is a protein-based traditional Chinese medicine made from donkey skins. Because it has the ability to nourish blood, its demand is increasing rapidly. The shortage of donkey skins increases the risk of the adulteration of CCA products with other animal skins. To ensure the drug efficacy and safety of CCA products, a proteomics technique was applied to reveal proteins in the skins of donkey, horse, cattle, and pig. Species-specific peptides for each animal species were predicted using bioinformatics, and their presence in the skins and gelatin samples was examined by nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS). One unique marker peptide for each animal species was selected to develop an LC-MS/MS multiple reaction monitoring method. The capability of this method to identify donkey, horse, cattle, and pig materials was demonstrated by analyzing in-house-made donkey gelatins containing different amounts of other animal skins and commercial CCA products. The adulteration of non-donkey species could be sensitively detected at a low level of 0.5%. Hybrid animals, such as mules and hinnies, were also differentiated from donkeys. We provide a practical tool for the quality control of CCA products. The strategy can also be used to study other important traditional Chinese medicines which contain animal proteins.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Colágeno/química
Proteínas/análise
Pele/química
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Animais
Biomarcadores/análise
Bovinos
Cromatografia Líquida/instrumentação
Colágeno/análise
Equidae
Gelatina/química
Cavalos
Medicina Tradicional Chinesa
Nanotecnologia/instrumentação
Nanotecnologia/métodos
Proteômica/instrumentação
Proteômica/métodos
Especificidade da Espécie
Suínos
Espectrometria de Massas em Tandem/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Proteins); 9000-70-8 (Gelatin); 9007-34-5 (Collagen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170703
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S136819


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[PMID]:28609464
[Au] Autor:Lee SR; Berlow EL; Ostoja SM; Brooks ML; Génin A; Matchett JR; Hart SC
[Ad] Endereço:U. S. Geological Survey, Western Ecological Research Center, Yosemite Field Station, Oakhurst, California, United States of America.
[Ti] Título:A multi-scale evaluation of pack stock effects on subalpine meadow plant communities in the Sierra Nevada.
[So] Source:PLoS One;12(6):e0178536, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We evaluated the influence of pack stock (i.e., horse and mule) use on meadow plant communities in Sequoia and Yosemite National Parks in the Sierra Nevada of California. Meadows were sampled to account for inherent variability across multiple scales by: 1) controlling for among-meadow variability by using remotely sensed hydro-climatic and geospatial data to pair stock use meadows with similar non-stock (reference) sites, 2) accounting for within-meadow variation in the local hydrology using in-situ soil moisture readings, and 3) incorporating variation in stock use intensity by sampling across the entire available gradient of pack stock use. Increased cover of bare ground was detected only within "dry" meadow areas at the two most heavily used pack stock meadows (maximum animals per night per hectare). There was no difference in plant community composition for any level of soil moisture or pack stock use. Increased local-scale spatial variability in plant community composition (species dispersion) was detected in "wet" meadow areas at the two most heavily used meadows. These results suggest that at the meadow scale, plant communities are generally resistant to the contemporary levels of recreational pack stock use. However, finer-scale within-meadow responses such as increased bare ground or spatial variability in the plant community can be a function of local-scale hydrological conditions. Wilderness managers can improve monitoring of disturbance in Sierra Nevada meadows by adopting multiple plant community indices while simultaneously considering local moisture regimes.
[Mh] Termos MeSH primário: Ecossistema
Equidae/fisiologia
Pradaria
Cavalos/fisiologia
Plantas/metabolismo
[Mh] Termos MeSH secundário: Altitude
Animais
California
Conservação de Recursos Naturais/métodos
Geografia
Parques Recreativos
Plantas/classificação
Dinâmica Populacional
Estações do Ano
Solo/química
Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 059QF0KO0R (Water)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178536


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[PMID]:28254582
[Au] Autor:Flores BJ; Pérez-Sánchez T; Fuertes H; Sheleby-Elías J; Múzquiz JL; Jirón W; Duttmann C; Halaihel N
[Ad] Endereço:Department of Animal Health, Centro Veterinario de Diagnóstico e Investigación (CEVEDI), School of Veterinary Medicine, Universidad Nacional Autónoma de Nicaragua-León, Carretera a la Ceiba 1 Km al Este, León, Nicaragua. Electronic address: byronfloressomarriba@gmail.com.
[Ti] Título:A cross-sectional epidemiological study of domestic animals related to human leptospirosis cases in Nicaragua.
[So] Source:Acta Trop;170:79-84, 2017 Jun.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Leptospirosis is one of the most extended zoonosis worldwide and humans become infected most commonly through contact with the urine of carrier animals, either directly or via contaminated water or soil. The aim in this study was to analyse the epidemiological behaviour of Leptospira spp., from domestic animals around the sites of human leptospirosis cases in Nicaragua, from 2007 through 2013. We report the results of a cross-sectional epidemiological study with a non-probability sampling of blood (n=3050) and urine (n=299) from Domestic Animals (DA) around the sites of human leptospirosis cases in Nicaragua. We analysed data obtained through Microscopic Agglutination Test (MAT), in-vitro culture, real time PCR and sequencing of lfb1 locus. Frequencies of 30.31% (95% CI: 28.66-31.95) and 15.38% (95% CI: 11.12-19.64) were obtained from serological test and from in-vitro culture, respectively. Although similar frequencies from serology test (P≥0.05) were found in DA species, in-vitro culture frequencies were significantly higher from bovine, equine and sheep (P<0.05) in comparison with swine and canine species. Ten serogroups of pathogenic Leptospira spp. were encountered, with the highest presence of Icterohaemorrhagiae serogroup 34.65% (95% CI: 29.35-39.94). We identified 7 samples homologous to L. interrogans species Pyrogenes serovar and 3 samples as L. noguchii Louisiana or Panama serovars by analysis of lfb1 sequences. We were able to establish a temporal and spatial correlation from DA and cumulative incidence of human cases. Therefore an effective epidemiological surveillance should be implemented with a specific control program toward DA in order to reduce human leptospirosis incidence.
[Mh] Termos MeSH primário: Animais Domésticos/microbiologia
Leptospira/classificação
Leptospirose/epidemiologia
[Mh] Termos MeSH secundário: Testes de Aglutinação/veterinária
Animais
Bovinos/microbiologia
Estudos Transversais
Cães/microbiologia
Equidae
Cavalos/microbiologia
Humanos
Nicarágua/epidemiologia
Reação em Cadeia da Polimerase em Tempo Real
Sorogrupo
Ovinos/microbiologia
Suínos/microbiologia
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE



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