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Pesquisa : B01.050.150.900.649.709.235 [Categoria DeCS]
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  1 / 1391 MEDLINE  
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[PMID]:28155284
[Au] Autor:Ndeereh D; Thaiyah A; Muchemi G; Miyunga AA
[Ad] Endereço:Department of Veterinary Services, Kenya Wildlife Service. dndeereh@kws.go.ke.
[Ti] Título:Molecular surveillance of spotted fever group rickettsioses in wildlife and detection of Rickettsia sibirica in a Topi (Damaliscus lunatus ssp. jimela) in Kenya.
[So] Source:Onderstepoort J Vet Res;84(1):e1-e7, 2017 Jan 30.
[Is] ISSN:2219-0635
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:Spotted fever group rickettsioses are a group of tick-borne zoonotic diseases caused by intracellular bacteria of the genus Rickettsia. The diseases are widely reported amongst international travellers returning from most sub-Saharan Africa with fever, yet their importance in local populations largely remains unknown. Although this has started to change and recently there have been increasing reports of the diseases in livestock, ticks and humans in Kenya, they have not been investigated in wildlife. We examined the presence, prevalence and species of Rickettsia present in wildlife in two regions of Kenya with a unique human-wildlife-livestock interface. For this purpose, 79 wild animals in Laikipia County and 73 in Maasai Mara National Reserve were sampled. DNA extracted from blood was tested using the polymerase chain reaction (PCR) to amplify the intergenic spacer rpmE-tRNAfMet and the citrate synthase-encoding gene gltA. Rickettsial DNA was detected in 2 of the 79 (2.5%) animals in Laikipia and 4 of the 73 (5.5%) in Maasai Mara. The PCR-positive amplicons of the gltA gene were sequenced to determine the detected Rickettsia species. This revealed Rickettsia sibirica in a Topi (Damaliscus lunatus ssp. jimela). This is the first report of spotted fever group rickettsioses in wildlife and the first to report R. sibirica in Kenya. The finding demonstrates the potential role of wild animals in the circulation of the diseases.
[Mh] Termos MeSH primário: Animais Selvagens/microbiologia
Antílopes/microbiologia
Infecções por Rickettsia/veterinária
Rickettsia
[Mh] Termos MeSH secundário: Animais
Búfalos/microbiologia
DNA Bacteriano/genética
Equidae/microbiologia
Humanos
Quênia/epidemiologia
Reação em Cadeia da Polimerase/veterinária
Vigilância da População
Prevalência
Rickettsia/genética
Infecções por Rickettsia/epidemiologia
Infecções por Rickettsia/transmissão
Zoonoses/epidemiologia
Zoonoses/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.4102/ojvr.v84i1.1265


  2 / 1391 MEDLINE  
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[PMID]:27981608
[Au] Autor:Ito H; Ogden R; Langenhorst T; Inoue-Murayama M
[Ad] Endereço:Wildlife Research Center, Kyoto University, Kyoto, Japan.
[Ti] Título:Contrasting results from molecular and pedigree-based population diversity measures in captive zebra highlight challenges facing genetic management of zoo populations.
[So] Source:Zoo Biol;36(1):87-94, 2017 Jan.
[Is] ISSN:1098-2361
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Zoo conservation breeding programs manage the retention of population genetic diversity through analysis of pedigree records. The range of demographic and genetic indices determined through pedigree analysis programs allows the conservation of diversity to be monitored relative to the particular founder population for a species. Such approaches are based on a number of well-documented founder assumptions, however without knowledge of actual molecular genetic diversity there is a risk that pedigree-based measures will be misinterpreted and population genetic diversity misunderstood. We examined the genetic diversity of the captive populations of Grevy's zebra, Hartmann's mountain zebra and plains zebra in Japan and the United Kingdom through analysis of mitochondrial DNA sequences. Very low nucleotide variability was observed in Grevy's zebra. The results were evaluated with respect to current and historic diversity in the wild, and indicate that low genetic diversity in the captive population is likely a result of low founder diversity, which in turn suggests relatively low wild genetic diversity prior to recent population declines. Comparison of molecular genetic diversity measures with analogous diversity indices generated from the studbook data for Grevy's zebra and Hartmann's mountain zebra show contrasting patterns, with Grevy's zebra displaying markedly less molecular diversity than mountain zebra, despite studbook analysis indicating that the Grevy's zebra population has substantially more founders, greater effective population size, lower mean kinship, and has suffered less loss of gene diversity. These findings emphasize the need to validate theoretical estimates of genetic diversity in captive breeding programs with empirical molecular genetic data. Zoo Biol. 36:87-94, 2017. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Animais de Zoológico
Cruzamento
Equidae/genética
Variação Genética
[Mh] Termos MeSH secundário: Criação de Animais Domésticos
Animais
Conservação de Recursos Naturais
Linhagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170221
[Lr] Data última revisão:
170221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE
[do] DOI:10.1002/zoo.21342


  3 / 1391 MEDLINE  
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[PMID]:28131384
[Au] Autor:Lafri I; Prat CM; Bitam I; Gravier P; Besbaci M; Zeroual F; Ben-Mahdi MH; Davoust B; Leparc-Goffart I
[Ad] Endereço:Institut des Sciences Vétérinaires, Université de Blida 1, Algeria.
[Ti] Título:Seroprevalence of West Nile virus antibodies in equids in the North-East of Algeria and detection of virus circulation in 2014.
[So] Source:Comp Immunol Microbiol Infect Dis;50:8-12, 2017 Feb.
[Is] ISSN:1878-1667
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:West Nile fever (WNF) is a viral disease of wild birds transmitted by mosquitoes. Humans and equids can also be affected and suffer from meningoencephalitis. In Algeria, since the 1994 epidemic, no data on WNV circulation was available until 2012. In September 2012, a fatal human case of WNV neuro-invasive infection occurred in Jijel province. This study describes the first seroprevalence study of West Nile virus (WNV) antibodies conducted in the equine population in Algeria. During 2014, serum samples were collected from 293 equids (222 donkeys and 71 horses) asymptomatic and unvaccinated for WNV in three localities in Northeastern wetlands of Algeria. Antibodies against WNV were found in 51 samples (seroprevalence 17.4%) of sampled equids, distributed as follows: 19 (seroprevalence 26.8%) horses and 32 (seroprevalence 14.4%) donkeys. Moreover 7 horses coming from Blida, in the center of Algeria, were tested before and after an 8-months stay in North-East Algeria. We observe a seroconversion in 2 horses, showing WNV circulation in 2014 in this specific region of Algeria.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Equidae
Doenças dos Cavalos/imunologia
Febre do Nilo Ocidental/veterinária
Vírus do Nilo Ocidental/imunologia
Vírus do Nilo Ocidental/isolamento & purificação
[Mh] Termos MeSH secundário: Argélia/epidemiologia
Animais
Animais Selvagens/imunologia
Animais Selvagens/virologia
Doenças Assintomáticas/epidemiologia
Culicidae/virologia
Ensaio de Imunoadsorção Enzimática/veterinária
Equidae/imunologia
Cavalos/imunologia
Humanos
Soroconversão
Estudos Soroepidemiológicos
Febre do Nilo Ocidental/epidemiologia
Febre do Nilo Ocidental/imunologia
Febre do Nilo Ocidental/virologia
Zonas Úmidas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170217
[Lr] Data última revisão:
170217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE


  4 / 1391 MEDLINE  
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[PMID]:27349090
[Au] Autor:Moll RJ; Killion AK; Montgomery RA; Tambling CJ; Hayward MW
[Ti] Título:Spatial patterns of African ungulate aggregation reveal complex but limited risk effects from reintroduced carnivores.
[So] Source:Ecology;97(5):1123-34, 2016 May.
[Is] ISSN:0012-9658
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The "landscape of fear" model, recently advanced in research on the non-lethal effects of carnivores on ungulates, predicts that prey will exhibit detectable antipredator behavior not only during risky times (i.e., predators in close proximity) but also in risky places (i.e., habitat where predators kill prey or tend to occur). Aggregation is an important antipredator response in numerous ungulate species, making it a useful metric to evaluate the strength and scope of the landscape of fear in a multi-carnivore, multi-ungulate system. We conducted ungulate surveys over a 2-year period in South Africa to test the influence of three broad-scale sources of variation in the landscape on spatial patterns in aggregation: (1) habitat structure, (2) where carnivores tended to occur (i.e., population-level utilization distributions), and (3) where carnivores tended to kill ungulate prey (i.e., probabilistic kill site maps). We analyzed spatial variation in aggregation for six ungulate species exposed to predation from recently reintroduced lion (Panthera leo) and spotted hyena (Crocuta crocuta). Although we did detect larger aggregations of ungulates in "risky places," these effects existed primarily for smaller-bodied (<150 kg) ungulates and were relatively moderate (change of 4 individuals across all habitats). In comparison, ungulate aggregations tended to increase at a slightly lower rate in habitat that was more open. The lion, an ambush (stalking) carnivore, had stronger influence on ungulate aggregation than the hyena, an active (coursing) carnivore. In addition, places where lions tended to kill prey had a greater effect on ungulate aggregation than places where lions tended to occur, but an opposing pattern existed for hyena. Our study reveals heterogeneity in the landscape of fear and suggests broad-scale risk effects following carnivore reintroduction only moderately influence ungulate aggregation size and vary considerably by predator hunting mode, type of predation risk, and prey species.
[Mh] Termos MeSH primário: Artiodáctilos/fisiologia
Equidae/fisiologia
Hyaenidae/fisiologia
Leões/fisiologia
Comportamento Predatório
[Mh] Termos MeSH secundário: Distribuição Animal/fisiologia
Animais
Modelos Biológicos
África do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160628
[Lr] Data última revisão:
160628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160628
[St] Status:MEDLINE


  5 / 1391 MEDLINE  
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[PMID]:27074044
[Au] Autor:Chamaillé-Jammes S; Charbonnel A; Dray S; Madzikanda H; Fritz H
[Ad] Endereço:Centre d'Ecologie Fonctionnelle et Evolutive, CNRS UMR 5175, 1919 route de Mende, 34294, Montpellier Cedex 5, France.
[Ti] Título:Spatial Distribution of a Large Herbivore Community at Waterholes: An Assessment of Its Stability over Years in Hwange National Park, Zimbabwe.
[So] Source:PLoS One;11(4):e0153639, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The spatial structuring of populations or communities is an important driver of their functioning and their influence on ecosystems. Identifying the (in)stability of the spatial structure of populations is a first step towards understanding the underlying causes of these structures. Here we studied the relative importance of spatial vs. interannual variability in explaining the patterns of abundance of a large herbivore community (8 species) at waterholes in Hwange National Park (Zimbabwe). We analyzed census data collected over 13 years using multivariate methods. Our results showed that variability in the census data was mostly explained by the spatial structure of the community, as some waterholes had consistently greater herbivore abundance than others. Some temporal variability probably linked to Park-scale migration dependent on annual rainfall was noticeable, however. Once this was accounted for, little temporal variability remained to be explained, suggesting that other factors affecting herbivore abundance over time had a negligible effect at the scale of the study. The extent of spatial and temporal variability in census data was also measured for each species. This study could help in projecting the consequences of surface water management, and more generally presents a methodological framework to simultaneously address the relative importance of spatial vs. temporal effects in driving the distribution of organisms across landscapes.
[Mh] Termos MeSH primário: Distribuição Animal/fisiologia
Migração Animal/fisiologia
Ecossistema
Herbivoria
[Mh] Termos MeSH secundário: Animais
Antílopes
Elefantes
Equidae
Girafas
Parques Recreativos
Água
Zimbábue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160423
[Lr] Data última revisão:
160423
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0153639


  6 / 1391 MEDLINE  
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[PMID]:26956100
[Au] Autor:Ibrahim A; Ali MM; Abou-Khalil NS; Ali MF
[Ad] Endereço:Department of Surgery, Anesthesiology and Radiology, Faculty of veterinary medicine, Assuit University, Assuit, 70155, Egypt. elgrah38@gmail.com.
[Ti] Título:Evaluation of chemical castration with calcium chloride versus surgical castration in donkeys: testosterone as an endpoint marker.
[So] Source:BMC Vet Res;12:46, 2016 Mar 08.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: For the last few years, researchers have been interested in developing a method for chemical sterilization which may be a better alternative to surgical castration. An ideal chemical sterilant would be one that effectively arrests spermatogenesis and androgenesis as well as libido with absence of toxic or other side effects. Calcium chloride in various solutions and concentrations has been tested in many animal species, but few studies have been evaluated it in equines as a chemical sterilant. So, the objective of this study was to evaluate the clinical efficacy of chemical castration with 20% calcium chloride dissolved in absolute ethanol in comparison with surgical castration in donkeys based on the changes in the serum testosterone level and the histopathological changes in treated testes. METHODS: Twelve clinically healthy adult male donkeys were used in this study. Donkeys were divided randomly and equally into two groups: a surgical (S) group (n = 6) and a chemical (C) group (n = 6). Animals in the (S) group were subjected to surgical castration while those in the (C) group received a single bilateral intratesticular injection of 20% calcium chloride dissolved in absolute ethanol (20 ml/testis). Animals were kept under clinical observation for 60 days. Changes in animals' behavior and gross changes in external genitalia were monitored daily. Serum concentrations of testosterone were measured prior to treatment and at 15, 30, 45 and 60 days post-treatment. Testicles in the (C) group were examined histopathologically at the end of the experiment. RESULTS: Chemical castration with intratesticular calcium chloride vs. surgical castration failed to reduce serum concentrations of testosterone throughout the whole duration of the study; however it induced orchitis that was evident by focal necrotic areas in seminiferous tubules, cellular infiltration of neutrophils, proliferative intertubular fibrosis with a compensatory proliferation of Leydig cells. Donkeys tolerated the intratesticular injection of calcium chloride. There were no detectable changes in the general health status of the animals with the exception of swelling in external genitalia, scrotal ulcerations and fistulas. Food and water consumption and the gait of animals remained unaffected. CONCLUSION: Intratesticular calcium chloride can't be considered an effective method for chemical castration in donkeys.
[Mh] Termos MeSH primário: Cloreto de Cálcio/farmacologia
Equidae
Orquiectomia/veterinária
Testículo/efeitos de drogas
[Mh] Termos MeSH secundário: Animais
Cloreto de Cálcio/administração & dosagem
Equidae/cirurgia
Masculino
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
M4I0D6VV5M (Calcium Chloride)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160311
[Lr] Data última revisão:
160311
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160309
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-016-0670-3


  7 / 1391 MEDLINE  
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[PMID]:26921034
[Au] Autor:Ligi M; Sengupta PP; Rudramurthy GR; Rahman H
[Ad] Endereço:National Institute of Veterinary Epidemiology & Disease Informatics (NIVEDI), Ramagondanahalli, Yelahanka, P.B. No. 6450, Bengaluru 560064, Karnataka, India.
[Ti] Título:Flagellar antigen based CI-ELISA for sero-surveillance of surra.
[So] Source:Vet Parasitol;219:17-23, 2016 Mar 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Trypanosoma evansi causes a disease known as 'surra' in wide range of domesticated and wild animals including cattle, buffaloes, horses, camels etc. The disease is transmitted through the bites of haematophagous tabanid flies and is characterized by undulating fever, chronic progressive weakness, and hypoglycemia leading to low productivity in animals. In the present study, monoclonal antibodies (MAbs) have been produced (IgG3 sub-type) against purified flagellar (FLA) protein of T. evansi and its immunoreactivity was evaluated by serological tests. MAb and purified protein were then exploited in the development of CI-ELISA and the diagnostic potentiality of the new ELISA test has been evaluated using 1230 sera samples from field animals including cattle, buffaloes, camels, horses and donkeys. The statistical analysis of the data showed optimum combination of sensitivity and specificity at 95.8 and 94.4, respectively. The positive-negative cut off percentage inhibition (PI) value was found to be >55, with a Cohen's Kappa value of 0.83. The study showed that the new assay has potential for application in sero-diagnosis as well as sero-surveillance of surra.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/sangue
Antígenos de Protozoários/metabolismo
Ensaio de Imunoadsorção Enzimática/veterinária
Vigilância da População/métodos
Tripanossomíase/veterinária
[Mh] Termos MeSH secundário: Animais
Antígenos de Protozoários/isolamento & purificação
Búfalos
Camelus
Bovinos
Linhagem Celular Tumoral
Ensaio de Imunoadsorção Enzimática/normas
Equidae
Flagelos/química
Cavalos
Camundongos
Sensibilidade e Especificidade
Estudos Soroepidemiológicos
Trypanosoma/fisiologia
Tripanossomíase/diagnóstico
Tripanossomíase/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Antigens, Protozoan)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160227
[St] Status:MEDLINE


  8 / 1391 MEDLINE  
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[PMID]:26904892
[Au] Autor:Campe A; Schulz S; Bohnet W
[Ti] Título:[Literature review of the influences on error rates when identifying equids with transponder and hot-iron branding].
[Ti] Título:Literaturübersicht zu den Einflussfaktoren auf die Fehlerwahrscheinlichkeiten bei der Identifikation von Equiden durch Transponder und Heißbrand..
[So] Source:Berl Munch Tierarztl Wochenschr;129(1-2):3-14, 2016 Jan-Feb.
[Is] ISSN:0005-9366
[Cp] País de publicação:Germany
[La] Idioma:ger
[Ab] Resumo:Although equids have had to be tagged with a transponder since 2009, breeding associations in Germany disagree as to which method is best suited for identification (with or without hot iron branding). Therefore, the aim of this systematic literature review was to gain an overview of how effective identification is using transponders and hot iron branding and as to which factors influence the success of identification. Existing literature showed that equids can be identified by means of transponders with a probability of 85-100%, whereas symbol brandings could be identified correctly in 78-89%, whole number brandings in 0-87% and single figures in 37-92% of the readings, respectively. The successful reading of microchips can be further optimised by a correctly operated implantation process and thorough training of the applying persons. affect identification with a scanner. The removal of transponders for manipulation purposes is virtually impossible. Influences during the application of branding marks can hardly, if at all, be standardised, but influence the subsequent readability relevantly. Therefore, identification by means of hot branding cannot be considered sufficiently reliable. Impaired quality of identification can be reduced during reading but cannot be counteracted. Based on the existing studies it can be concluded that the transponder method is the best suited of the investigated methods for clearly identifying equids, being forgery-proof and permanent. It is not to be expected that applying hot branding in addition to microchips would optimise the probability of identification relevantly.
[Mh] Termos MeSH primário: Sistemas de Identificação Animal/normas
Equidae/classificação
[Mh] Termos MeSH secundário: Sistemas de Identificação Animal/métodos
Animais
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1606
[Cu] Atualização por classe:160224
[Lr] Data última revisão:
160224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160224
[St] Status:MEDLINE


  9 / 1391 MEDLINE  
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[PMID]:26849100
[Au] Autor:Ter Beest JM; Schook MW
[Ad] Endereço:The Wilds, Cumberland, Ohio.
[Ti] Título:Estrous cycle synchronization in the Persian onager (Equus hemionus onager).
[So] Source:Zoo Biol;35(2):87-94, 2016 Mar-Apr.
[Is] ISSN:1098-2361
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The endangered Persian onager (Equus hemionus onager) has experienced significant population declines over the past century due to poaching, habitat destruction, and resource competition. Remaining animals in zoos and the wild are regionally isolated. Artificial insemination (AI) may be particularly useful as a means of aiding in global genetic management of these isolated populations. The first successful AI in onagers was performed in 2009 utilizing urinary hormone analyses and regular transrectal ultrasound examinations that required specialized handling devices. A method for estrous synchronization in this species would alleviate the need for daily handling and provide a more feasible approach to AI. This study tested long-acting controlled-release preparations of estradiol and progesterone, followed by a single injection of prostaglandin 10 days later, in six adult female Persian onagers to determine whether ovulation would occur within a narrow window of time. Serial transrectal ultrasound exams were performed to determine the day of ovulation following hormone treatment. Means and standard deviations were determined for the lengths of follicular and luteal phases, follicle sizes, and time to ovulation, and compared to historical data in this species. All six onagers ovulated between Days 18 and 22, with three females ovulating on Day 19, as determined by the presence of a corpus luteum. This is an apparently safe and effective method for the synchronization of estrous cycles in the Persian onager, and may be used to develop a timed AI protocol for use at institutions that do not have specialized handling facilities to enable regular transrectal ultrasound.
[Mh] Termos MeSH primário: Animais de Zoológico/fisiologia
Equidae/fisiologia
Sincronização do Estro
Inseminação Artificial/veterinária
[Mh] Termos MeSH secundário: Animais
Espécies em Perigo de Extinção
Feminino
Ultrassonografia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160411
[Lr] Data última revisão:
160411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160411
[St] Status:MEDLINE
[do] DOI:10.1002/zoo.21265


  10 / 1391 MEDLINE  
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[PMID]:26842878
[Au] Autor:Wang XF; Lin YZ; Li Q; Liu Q; Zhao WW; Du C; Chen J; Wang X; Zhou JH
[Ad] Endereço:State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China. xuefengwang1982@126.com.
[Ti] Título:Genetic Evolution during the development of an attenuated EIAV vaccine.
[So] Source:Retrovirology;13:9, 2016 Feb 03.
[Is] ISSN:1742-4690
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The equine infectious anemia virus (EIAV) vaccine is the only attenuated lentiviral vaccine applied on a large scale that has been shown to be effective in controlling the prevalence of EIA in China. This vaccine was developed by successive passaging of a field-isolated virulent strain in different hosts and cultivated cells. To explore the molecular basis for the phenotype alteration of this vaccine strain, we systematically analyzed its genomic evolution during vaccine development. RESULTS: Sequence analysis revealed that the genetic distance between the wild-type strain and six representative strains isolated from key development stages gradually increased with the number of passages. Env gene, but not gag and pol, showed a clear evolutionary flow similar to that of the whole genomes of different generations during the attenuation. Stable mutations were identified in multiple regions of multiple genes along with virus passaging. The adaption of the virus to the growth environment of cultured cells with accumulated genomic and genetic variations was positively correlated with the reduction in pathogenicity and rise of immunogenicity. Statistical analyses revealed significant differences in the frequency of the most stable mutations between in vivo and ex vivo-adapted strains and between virulent and attenuated strains. CONCLUSIONS: These data indicate that EIAV evolution during vaccine development generated an accumulation of mutations under the selective drive force, which helps to better understand the molecular basis of lentivirus pathogenicity and immunogenicity.
[Mh] Termos MeSH primário: Anemia Infecciosa Equina/prevenção & controle
Evolução Molecular
Vírus da Anemia Infecciosa Equina/imunologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
China
Equidae
Cavalos
Vírus da Anemia Infecciosa Equina/genética
Vírus da Anemia Infecciosa Equina/patogenicidade
Dados de Sequência Molecular
Mutação
Análise de Sequência de DNA
Inoculações Seriadas
Vacinas Atenuadas/genética
Vacinas Atenuadas/imunologia
Vacinas Atenuadas/isolamento & purificação
Vacinas Virais/genética
Vacinas Virais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Vaccines, Attenuated); 0 (Viral Vaccines)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161111
[Lr] Data última revisão:
161111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE
[do] DOI:10.1186/s12977-016-0240-6



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