Base de dados : MEDLINE
Pesquisa : B01.050.500.091.426.400 [Categoria DeCS]
Referências encontradas : 201 [refinar]
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  1 / 201 MEDLINE  
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[PMID]:29244473
[Au] Autor:Rotkina AS; Pronina IV; Lazarev VN; Akhaev DN; Baskova IP
[Ti] Título:Destabilase-lysozyme-2 - original recombinant thrombolytic preparation of medicinal leech inhibits horse platelets aggregation.
[So] Source:Patol Fiziol Eksp Ter;60(3):47-51, 2016 Jul-Sep.
[Is] ISSN:0031-2991
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The purpose. Identifying the capacity of the medicinal leech novel original recombinant thrombolytic preparation Destabilase-Lysozyme-2 to inhibit the blood platelet aggregation. Methods: Gene of destabilase-lysozyme. ds2 (mlDL-Ds2 ), was cloned in E.coli cells. Recombinant protein was isolated in denaturing conditions using metal-chelate chromatography followed by denaturation of the polypeptide by rapid dilution in exact accordance with the procedure described by Kurdyumov A.S. et al. ( 2016, Russian Journal of Bioorganic Chemistry, v.42, s. 42-52). Blood was collected from the jugular vein of 18 horses. The functional status of platelets in the presence of different destabilase-lysozyme concentrations were evaluated for their aggregation in Platelet Rich Plasma ( PRP) and in Washed Platelet suspension (WP) using aggregometers Chrono-Log-700 and Сhrono-Log-560, USA560, США. As used aggregation inducers of ADP, collagen type III and human thrombin. Results: First demonstrated the ability of newly synthesized (Kurdyumov A.S. et al. 2016, Russian Journal of Bioorganic Chemistry, v42, s. 42-52) thrombolytic recombinant enzyme destabilase-lyzosyme to inhibit more than 40% of ADP-stimulated PRP aggregation and ADP- stimulated aggregation of horse blood washed platelets. Conclusion: The ability of destabilase-lyzosyme -2 to inhibit platelets aggregation extends biological properties of recombinant thrombolytic enzyme, pre-clinical trials which resulted in the end of 2015.
[Mh] Termos MeSH primário: Plaquetas/metabolismo
Endopeptidases
Fibrinolíticos
Hirudo medicinalis/enzimologia
Muramidase
Agregação Plaquetária/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Endopeptidases/química
Endopeptidases/isolamento & purificação
Endopeptidases/farmacologia
Fibrinolíticos/química
Fibrinolíticos/isolamento & purificação
Fibrinolíticos/farmacologia
Cavalos
Muramidase/química
Muramidase/isolamento & purificação
Muramidase/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fibrinolytic Agents); EC 3.2.1.17 (Muramidase); EC 3.4.- (Endopeptidases); EC 3.4.99.- (fibrin destabilase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE


  2 / 201 MEDLINE  
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[PMID]:28343561
[Au] Autor:Hitchcock AC; Connolly EM; Darakananda K; Jeong JW; Quist AJ; Robbins AB; Ellerby DJ
[Ad] Endereço:Department of Biological Sciences, Wellesley College, 106 Central Street, Wellesley, MA 02481, USA.
[Ti] Título:Locomotor and energetic consequences of behavioral thermoregulation in the sanguivorous leech Hirudo verbana.
[So] Source:J Therm Biol;65:1-7, 2017 Apr.
[Is] ISSN:0306-4565
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Medicinal leeches (Hirudo verbana) thermoregulate with respect to their sanguivorous feeding behavior. Immediate postprandial preferences are for warmer than their initial acclimation temperature (T , 21°C, Petersen et al. 2011), while unfed leeches have a lower preferred temperature (T , 12.5°C). This may reduce energy expenditure and defer starvation if feeding opportunities are limited. Energetic benefits may have an associated cost if low temperatures reduce mobility and the ability to locate further hosts. These costs could be limited if mobility is unimpaired at low temperatures, or if acclimation can restore locomotor performance to the levels at T . The transition from T to the unfed T significantly reduced speed and propulsive cycle frequency during swimming, and extension and retraction rates during crawling. Aerobic metabolic rate was also reduced from 0.20±0.03Wkg at T to 0.10±0.03Wkg at T . The Q values of 1.7-2.9 for energetic and swimming parameters indicate a substantial temperature effect, although part of the decline in swimming performance can be attributed to temperature-related changes in water viscosity. 6 weeks at T resulted in no detectable acclimation in locomotor performance or aerobic metabolism. The energetic savings associated with a lower T in unfed leeches effectively doubled the estimated time until depletion of energy reserves. Given that some mobility is still retained at T , and that acclimation is in itself costly, the energetic benefits of selecting cooler temperatures between feedings may outweigh the costs associated with reduced locomotor performance.
[Mh] Termos MeSH primário: Regulação da Temperatura Corporal
Hirudo medicinalis/fisiologia
[Mh] Termos MeSH secundário: Aclimatação
Animais
Temperatura Baixa
Metabolismo Energético
Comportamento Alimentar
Locomoção
Natação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170510
[Lr] Data última revisão:
170510
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE


  3 / 201 MEDLINE  
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[PMID]:27687675
[Au] Autor:Salimi B; Abdi K
[Ad] Endereço:a Department of Aquatic Animal Health and Disease, Faculty of Veterinary Medicine , Sanandaj Branch, Islamic Azad University, Pasdaran Boulevard , Post Office Box 618, Sanandaj , Iran.
[Ti] Título:Detection of Infectious Pancreatic Necrosis Virus from the Leeches Hemiclepsis marginata and Hirudo medicinalis.
[So] Source:J Aquat Anim Health;28(4):209-13, 2016 12.
[Is] ISSN:1548-8667
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Leeches have been reported to harbor several important fish pathogens, including spring viremia of carp virus, infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV), and also may contain blood protozoa. In the present study, leeches were collected from water bodies located in Kurdistan province, Iran. The specimens were tested for IHNV, VHSV, and infectious pancreatic necrosis virus (IPNV) using the PCR method. The results showed that two different species of leeches, Hemiclepsis marginata and Hirudo medicinalis, were infected by IPNV among the seven species studied. The infected leeches were found in areas that were polluted with untreated sewage coming from upstream fish farms culturing Rainbow Trout Oncorhynchus mykiss. In addition, the fish at fish farms in the vicinity had been infected with IPNV 9 months previously. Our results showed that the virus causing infectious pancreatic necrosis is present in the leeches H. marginata and H. medicinalis, suggesting that leeches are a potential source of IPNV in fish farms. Received October 14, 2015; accepted June 1, 2016.
[Mh] Termos MeSH primário: Vírus da Necrose Pancreática Infecciosa/isolamento & purificação
Sanguessugas/virologia
[Mh] Termos MeSH secundário: Animais
Aquicultura
Hirudo medicinalis/virologia
Vírus da Necrose Hematopoética Infecciosa/isolamento & purificação
Vírus da Necrose Pancreática Infecciosa/genética
Irã (Geográfico)
Novirhabdovirus/genética
Novirhabdovirus/isolamento & purificação
Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161001
[St] Status:MEDLINE
[do] DOI:10.1080/08997659.2016.1206635


  4 / 201 MEDLINE  
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[PMID]:27636374
[Au] Autor:Pipkin JE; Bushong EA; Ellisman MH; Kristan WB
[Ad] Endereço:Neurosciences Graduate Program, University of California at San Diego, La Jolla, California, 92093. jason.e.pipkin@gmail.com.
[Ti] Título:Patterns and distribution of presynaptic and postsynaptic elements within serial electron microscopic reconstructions of neuronal arbors from the medicinal leech Hirudo verbana.
[So] Source:J Comp Neurol;524(18):3677-3695, 2016 Dec 15.
[Is] ISSN:1096-9861
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microscale connectomics involves the large-scale acquisition of high-resolution serial electron micrographs from which neuronal arbors can be reconstructed and synapses can be detected. In addition to connectivity information, these data sets are also rich with structural information, including vesicle types, number of postsynaptic partners at a given presynaptic site, and spatial distribution of synaptic inputs and outputs. This study uses serial block-face scanning electron microscopy (EM) to collect two volumes of serial EM data from ganglia of the medicinal leech. For the first volume, we sampled a small fraction of the neuropil belonging to an adult ganglion. From this data set we measured the proportion of arbors that contained vesicles and the types of vesicles contained and developed criteria to identify synapses and to measure the number of apparent postsynaptic partners in apposition to presynaptic boutons. For the second data set, we sampled an entire juvenile ganglion, which included the somata and arbors of all the neurons. We used this data set to placd our findings from mature tissue in the context of fully reconstructed arbors and to explore the spatial distribution of synaptic inputs and outputs on these arbors. We observed that some neurons segregated their arbors into input only and mixed input/output zones, that other neurons contained exclusively mixed input/output zones, and that still others contained only input zones. These results provide the groundwork for future behavioral studies. J. Comp. Neurol. 524:3677-3695, 2016. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Hirudo medicinalis/anatomia & histologia
Sinapses/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Gânglios dos Invertebrados/ultraestrutura
Imagem Tridimensional
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160917
[St] Status:MEDLINE
[do] DOI:10.1002/cne.24120


  5 / 201 MEDLINE  
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[PMID]:27494593
[Au] Autor:Larsson J; Klasson S; Arnljots B
[Ad] Endereço:Section for Plastic Surgery, SUS Skåne University Hospital, Malmö, Sweden.
[Ti] Título:Successful Nose Replantation Using Leeches for Venous Draining.
[So] Source:Facial Plast Surg;32(4):469-70, 2016 Aug.
[Is] ISSN:1098-8793
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Mordeduras e Picadas/cirurgia
Cães
Drenagem/métodos
Hirudo medicinalis
Nariz/cirurgia
Reimplante
[Mh] Termos MeSH secundário: Idoso
Animais
Feminino
Seres Humanos
Nariz/irrigação sanguínea
Nariz/lesões
Reimplante/métodos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:160806
[St] Status:MEDLINE
[do] DOI:10.1055/s-0036-1584235


  6 / 201 MEDLINE  
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[PMID]:27021369
[Au] Autor:Chernaya LV; Kovalchuk LA; Nokhrina ES
[Ad] Endereço:Institute of Plant and Animal Ecology, Ural Branch, Russian Academy of Sciences, Yekaterinburg, Russia. chernaya_lv@mail.ru.
[Ti] Título:Role of the tissue free amino acids in adaptation of medicinal leeches Hirudo medicinalis L., 1758 to extreme climatic conditions.
[So] Source:Dokl Biol Sci;466:42-4, 2016.
[Is] ISSN:1608-3105
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The first comparison of the spectra of free amino acids in tissues of the medicinal leeches H. medicinalis from different climatic and geographical Eurasian areas has been performed. Adaptation of H. medicinalis to extreme climatic conditions occurs via intensification of the amino acid metabolism resulting from a significant increase in the content of essential amino acids. Accumulation of arginine, histidine, and lysine (3.6-, 3.9-, and 2.0-fold increases, respectively) has proved to play a special protective role in adaptation of H. medicinalis to the low positive temperatures.
[Mh] Termos MeSH primário: Aclimatação/fisiologia
Hirudo medicinalis/fisiologia
[Mh] Termos MeSH secundário: Aclimatação/genética
Animais
Arginina/metabolismo
Hirudo medicinalis/genética
Hirudo medicinalis/metabolismo
Histidina/metabolismo
Lisina/metabolismo
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
4QD397987E (Histidine); 94ZLA3W45F (Arginine); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160330
[St] Status:MEDLINE
[do] DOI:10.1134/S0012496616010129


  7 / 201 MEDLINE  
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[PMID]:26267058
[Au] Autor:Müller C; Mescke K; Liebig S; Mahfoud H; Lemke S; Hildebrandt JP
[Ad] Endereço:Animal Physiology and Biochemistry, Zoological Institute and Museum, Ernst Moritz Arndt-University, Greifswald, Germany. christian.mueller@uni-greifswald.de.
[Ti] Título:More than just one: multiplicity of Hirudins and Hirudin-like Factors in the Medicinal Leech, Hirudo medicinalis.
[So] Source:Mol Genet Genomics;291(1):227-40, 2016 Feb.
[Is] ISSN:1617-4623
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Blood-sucking leeches like the medicinal leech, Hirudo medicinalis, have been used for medical purposes since ancient times. During feeding, medicinal leeches transfer a broad range of bioactive substances into the host's wound to prevent premature hemostasis and blood coagulation. Hirudin is probably the best known of these substances. Despite its long history of investigation, recombinant production and clinical use, there still exist conflicting data regarding the primary structure of hirudin. Entirely unclear is the potential biological significance of three different subtypes and many isoforms of hirudins that have been characterized so far. Furthermore, there is only incomplete information on their cDNA sequences and no information at all on gene structures and DNA sequences are available in the databases. Our efforts to fill these gaps revealed the presence of multiple hirudin-encoding genes in the genome of Hirudo medicinalis. We have strong evidence for the expression of all three subtypes of hirudin within individual leeches and for the expression of additional hirudins or hirudin-like factors that may have different biological functions and may be promising candidates for new drugs.
[Mh] Termos MeSH primário: Hirudinas/genética
Hirudo medicinalis/genética
Sanguessugas/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Dados de Sequência Molecular
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hirudins)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150813
[St] Status:MEDLINE
[do] DOI:10.1007/s00438-015-1100-0


  8 / 201 MEDLINE  
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[PMID]:26636582
[Au] Autor:Girardello R; Tasselli S; Baranzini N; Valvassori R; de Eguileor M; Grimaldi A
[Ad] Endereço:Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
[Ti] Título:Effects of Carbon Nanotube Environmental Dispersion on an Aquatic Invertebrate, Hirudo medicinalis.
[So] Source:PLoS One;10(12):e0144361, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The recent widespread applications of nanomaterials, because of their properties, opens new scenarios that affect their dispersal in the environment. In particular multiwall carbon nanotubes (MWCNTs), despite their qualities, seem to be harmful for animals and humans. To evaluate possible toxic effects caused by carbon nanotube environmental dispersion, with regard to aquatic compartment, we proposed as experimental model a freshwater invertebrate: Hirudo medicinalis. In the present study we analyse acute and chronic immune responses over a short (1, 3, 6 and 12 hours) and long time (from 1 to 5 weeks) exposure to MWCNTs by optical, electron and immunohistochemical approaches. In the exposed leeches angiogenesis and fibroplasia accompanied by massive cellular migration occur. Immunocytochemical characterization using specific markers shows that in these inflammatory processes the monocyte-macrophages (CD45+, CD68+) are the most involved cells. These immunocompetent cells are characterized by sequence of events starting from the expression of pro-inflammatory cytokines (in particular IL-18), and amyloidogenensis. Our combined experimental approaches, basing on high sensitive inflammatory response can highlight adverse effects of nanomaterials on aquatic organisms and could be useful to assess the MWCNTs impact on aquatic, terrestrial animal and human health.
[Mh] Termos MeSH primário: Antígenos CD/imunologia
Antígenos de Diferenciação Mielomonocítica/imunologia
Hirudo medicinalis/imunologia
Antígenos Comuns de Leucócito/imunologia
Macrófagos/imunologia
Monócitos/imunologia
Nanotubos de Carbono/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Interleucina-18/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, Myelomonocytic); 0 (CD68 antigen, human); 0 (Interleukin-18); 0 (Nanotubes, Carbon); EC 3.1.3.48 (Leukocyte Common Antigens)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151205
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0144361


  9 / 201 MEDLINE  
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[PMID]:26589324
[Au] Autor:Kurdyumov AS; Manuvera VA; Baskova IP; Lazarev VN
[Ad] Endereço:Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya, 1a, Moscow, 119435, Russia. aleks-kuzmaland@bk.ru.
[Ti] Título:A comparison of the enzymatic properties of three recombinant isoforms of thrombolytic and antibacterial protein--Destabilase-Lysozyme from medicinal leech.
[So] Source:BMC Biochem;16:27, 2015 Nov 21.
[Is] ISSN:1471-2091
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Destabilase-Lysozyme (mlDL) is a multifunctional i-type enzyme that has been found in the secretions from the salivary glands of medicinal leeches. mlDL has been shown to exhibit isopeptidase, muramidase and antibacterial activity. This enzyme attracts interest because it expresses thrombolytic activity through isopeptidolysis of the ε-(γ-Glu)-Lys bonds that cross-link polypeptide chains in stabilised fibrin. To date, three isoforms of mlDL have been identified. The enzymatic properties of pure mlDL isoforms have not yet been described because only destabilase complexes containing other proteins could be isolated from the salivary gland secretion and because low product yield from the generation of recombinant proteins has made comprehensive testing difficult. RESULTS: In the present study, we optimised the procedures related to the expression, isolation and purification of active mlDL isoforms (mlDL-Ds1, mlDL-Ds2, mlDL-Ds3) using an Escherichia coli expression system, and we detected and compared their muramidase, lytic, isopeptidase and antimicrobial activities. After optimisation, the product yield was 30 mg per litre of culture. The data obtained in our study led to the suggestion that the recombinant mlDL isoforms isolated from inclusion bodies form stable oligomeric complexes. Analyses of the tested activities revealed that all isoforms exhibited almost identical patterns of pH and ionic strength effects on the activities. We determined that mlDL-Ds1, 2, 3 possessed non-enzymatic antibacterial activity independent of their muramidase activity. For the first time, we demonstrated the fibrinolytic activity of the recombinant mlDL and showed that only intact proteins possessed this activity, suggesting their enzymatic nature. CONCLUSIONS: The recombinant Destabilase-Lysozyme isoforms obtained in our study may be considered potential thrombolytic agents that act through a mechanism different from that of common thrombolytics.
[Mh] Termos MeSH primário: Endopeptidases/metabolismo
Hirudo medicinalis/enzimologia
Muramidase/metabolismo
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Antibacterianos/isolamento & purificação
Antibacterianos/metabolismo
Antibacterianos/farmacologia
Bacillus subtilis/efeitos dos fármacos
Bacillus subtilis/crescimento & desenvolvimento
Cromatografia de Afinidade
Cromatografia em Gel
Dicroísmo Circular
Estabilidade de Medicamentos
Endopeptidases/genética
Endopeptidases/isolamento & purificação
Endopeptidases/farmacologia
Estabilidade Enzimática
Escherichia coli/efeitos dos fármacos
Escherichia coli/crescimento & desenvolvimento
Fibrinolíticos/isolamento & purificação
Fibrinolíticos/metabolismo
Fibrinolíticos/farmacologia
Concentração de Íons de Hidrogênio
Isoenzimas/genética
Isoenzimas/isolamento & purificação
Isoenzimas/metabolismo
Isoenzimas/farmacologia
Testes de Sensibilidade Microbiana
Muramidase/genética
Muramidase/isolamento & purificação
Muramidase/farmacologia
Concentração Osmolar
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/isolamento & purificação
Fragmentos de Peptídeos/metabolismo
Fragmentos de Peptídeos/farmacologia
Mapeamento de Peptídeos
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
Proteínas Recombinantes/farmacologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Fibrinolytic Agents); 0 (Isoenzymes); 0 (Peptide Fragments); 0 (Recombinant Proteins); EC 3.2.1.17 (Muramidase); EC 3.4.- (Endopeptidases); EC 3.4.99.- (fibrin destabilase)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151123
[Lr] Data última revisão:
151123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151122
[St] Status:MEDLINE
[do] DOI:10.1186/s12858-015-0056-3


  10 / 201 MEDLINE  
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[PMID]:26427330
[Au] Autor:Liu C; Barkley TW
[Ad] Endereço:Celina Liu is a recent graduate of the Adult-Gerontology Acute Care Nurse Practitioner Program at California State University, Los Angeles School of Nursing, and is working on her board certification exam. Thomas Barkley, Jr., was Director of Nurse Practitioner Programs when this article was written.
[Ti] Título:Medicinal leech therapy: New life for an ancient treatment.
[So] Source:Nursing;45(11):25-30; quiz 30-1, 2015 Nov.
[Is] ISSN:1538-8689
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Terapia com Hirudina/enfermagem
Aplicação de Sanguessugas/enfermagem
Medicina Tradicional
[Mh] Termos MeSH secundário: Animais
Hirudo medicinalis
Seres Humanos
Medição de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1604
[Cu] Atualização por classe:151014
[Lr] Data última revisão:
151014
[Sb] Subgrupo de revista:N
[Da] Data de entrada para processamento:151003
[St] Status:MEDLINE
[do] DOI:10.1097/01.NURSE.0000472561.41030.2d



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