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  1 / 1670 MEDLINE  
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[PMID]:29337391
[Au] Autor:Ishii T; Niikura Y; Kurata K; Muroi M; Tanamoto K; Nagase T; Sakaguchi M; Yamashita N
[Ad] Endereço:Department of Pharmacotherapy, Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo, Japan.
[Ti] Título:Time-dependent distinct roles of Toll-like receptor 4 in a house dust mite-induced asthma mouse model.
[So] Source:Scand J Immunol;87(3), 2018 Mar.
[Is] ISSN:1365-3083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:House dust mites (HDMs) are a common source of allergens that trigger both allergen-specific and innate immune responses in humans. Here, we examined the effect of allergen concentration and the involvement of Toll-like receptor 4 (TLR4) in the process of sensitization to house dust mite allergens in an HDM extract-induced asthma mouse model. Intranasal administration of HDM extract induced an immunoglobulin E response and eosinophilic inflammation in a dose-dependent manner from 2.5 to 30 µg/dose. In TLR4-knockout mice, the infiltration of eosinophils and neutrophils into the lung was decreased compared with that in wild-type mice in the early phase of inflammation (total of three doses). However, in the late phase of inflammation (total of seven doses), eosinophil infiltration was significantly greater in TLR4-knockout mice than in wild-type mice. This suggests that the roles of TLR4 signaling are different between the early phase and the later phase of HDM allergen-induced inflammation. Thus, innate immune response through TLR4 regulated the response to HDM allergens, and the regulation was altered during the phase of inflammation.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Antígenos de Dermatophagoides/imunologia
Asma/imunologia
Imunidade Inata/imunologia
Pyroglyphidae/imunologia
Receptor 4 Toll-Like/imunologia
[Mh] Termos MeSH secundário: Resistência das Vias Respiratórias/imunologia
Animais
Líquido da Lavagem Broncoalveolar/citologia
Modelos Animais de Doenças
Eosinófilos/patologia
Feminino
Imunização
Imunoglobulina E/imunologia
Inflamação/imunologia
Pulmão/citologia
Pulmão/imunologia
Pulmão/patologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Infiltração de Neutrófilos/imunologia
Neutrófilos/patologia
Transdução de Sinais/imunologia
Receptor 4 Toll-Like/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Antigens, Dermatophagoides); 0 (Tlr4 protein, mouse); 0 (Toll-Like Receptor 4); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1111/sji.12641


  2 / 1670 MEDLINE  
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[PMID]:28452705
[Au] Autor:Lin L; Chen Z; Cao Y; Sun G
[Ad] Endereço:Department of Otorhinolaryngology-Head and Neck Surgery, Huashan Hospital of Fudan University, Shanghai, China.
[Ti] Título:Normal saline solution nasal-pharyngeal irrigation improves chronic cough associated with allergic rhinitis.
[So] Source:Am J Rhinol Allergy;31(2):96-104, 2017 Mar 01.
[Is] ISSN:1945-8932
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Upper airway inflammation is one of the most commonly identified causes of chronic cough, although the underlying mechanism is not clear. This study compared normal saline solution nasal-pharyngeal irrigation (NSNPI) and fluticasone propionate nasal spray (FPNS) treatment for chronic cough associated with allergic rhinitis (AR). METHODS: Patients with suspected AR to house-dust mite were enrolled, and the symptom of cough was assessed by a cough symptom score and the Leicester Cough Questionnaire, and cough response to capsaicin was evaluated. AR was assessed by using the visual analog scale (VAS) and the Mini Juniper Rhinoconjunctivitis Quality of Life Questionnaire (MiniRQLQ). Mediators, including histamine, leukotriene C4, and prostaglandin D2, and the major basic protein from nasal lavage fluid (NLF) were examined. The patients were treated with NSNPI (the NSNPI group) or FPNS (the FPNS group) for 30 days, after which they were reassessed. RESULTS: Forty-five of 50 patients completed this study. The scores of the cough symptom and the Leicester Cough Questionnaire, and the capsaicin cough threshold all improved statistically after NSNPI but did not change after FPNS. There were statistically significant changes in the evaluations of the MiniRQLQ and the mediators, including histamine and leukotriene C4, in the NLF in the NSNPI group. However, significant changes were found in the assessments of VAS, MiniRQLQ, and all above mediators including histamine, leukotriene C4, and prostaglandin D2, and the major basic protein in the NLF of the FPNS group. Furthermore, the assessments of VAS and all the mediators were reduced more in the FPNS group compared with those in the NSNPI group. CONCLUSION: The patients with suspected AR to house-dust mite reported a better relief of the cough symptom after 30 days of treatment with NSNPI compared with that after nasal corticosteroid.
[Mh] Termos MeSH primário: Tonsila Faríngea/patologia
Tosse/prevenção & controle
Fluticasona/uso terapêutico
Seios Paranasais/patologia
Rinite Alérgica/terapia
Cloreto de Sódio/uso terapêutico
Irrigação Terapêutica
[Mh] Termos MeSH secundário: Tonsila Faríngea/efeitos dos fármacos
Adolescente
Adulto
Idoso
Animais
Antígenos de Dermatophagoides/imunologia
Doença Crônica
Tosse/etiologia
Seres Humanos
Meia-Idade
Sprays Nasais
Seios Paranasais/efeitos dos fármacos
Pyroglyphidae/imunologia
Rinite Alérgica/complicações
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Antigens, Dermatophagoides); 0 (Nasal Sprays); 451W47IQ8X (Sodium Chloride); CUT2W21N7U (Fluticasone)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.2500/ajra.2017.31.4418


  3 / 1670 MEDLINE  
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[PMID]:27778095
[Au] Autor:He S; Mou Z; Peng L; Chen J
[Ad] Endereço:Department of Otorhinolaryngology, Shanghai Children's Medical Center, affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, 200127, China.
[Ti] Título:Impacts of meteorological and environmental factors on allergic rhinitis in children.
[So] Source:Int J Biometeorol;61(5):797-806, 2017 May.
[Is] ISSN:1432-1254
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Meteorological and environmental factors influence the pathogenesis of allergic rhinitis (AR). An understanding of the risk factors will facilitate the development of diagnostic and preventative tools for AR children and improve their quality of life. However, research on the impact of these factors on subjective symptoms in AR children remains scarce. This study explored the relationships between subjective symptoms in pollen and dust mite positive AR children, and meteorological and environmental factors. Using a linear mixed effect model, we analyzed the correlations between monthly data on the subjective symptoms of 351 AR children (from the Shanghai Children's Medical Center) and meteorological and environmental factors during 2013. The monthly meteorological and environmental data were provided by the Shanghai Meteorological Service and Shanghai Environmental Protection Bureau. Temperature and humidity were negatively correlated with the subjective symptom score, with a 0.04 point increase observed for every 1 °C decrease in temperature (P < 0.0001) or 10 % decline in humidity (P = 0.0412). The particulate matter (PM) 10 and PM2.5 concentrations were positively correlated with the subjective symptom score, with a 10 µg/m increase in PM10 and PM2.5 yielding a 0.02 (P = 0.0235) and 0.03 (P = 0.0281) increase in the subjective symptom score, respectively. In conclusion, meteorological and environmental factors were correlated with subjective symptoms in AR children. Low temperatures, lower humidity, and high PM10 and PM2.5 concentrations aggravated subjective symptoms in AR children.
[Mh] Termos MeSH primário: Rinite Alérgica/etiologia
[Mh] Termos MeSH secundário: Adolescente
Poluentes Atmosféricos
Alérgenos/imunologia
Animais
Criança
Pré-Escolar
China/epidemiologia
Feminino
Seres Humanos
Umidade
Modelos Lineares
Masculino
Material Particulado
Pólen/imunologia
Pyroglyphidae/imunologia
Rinite Alérgica/epidemiologia
Fatores de Risco
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Allergens); 0 (Particulate Matter)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1007/s00484-016-1257-1


  4 / 1670 MEDLINE  
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[PMID]:29192831
[Au] Autor:Johnson JR; Harker JA
[Ad] Endereço:1 School of Life and Health Sciences Aston University Birmingham, United Kingdom and.
[Ti] Título:Allergic Airway Disease: More than Meets the IgE?
[So] Source:Am J Respir Cell Mol Biol;57(6):631-632, 2017 12.
[Is] ISSN:1535-4989
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Alérgenos/imunologia
Asma/imunologia
Imunoglobulina E/imunologia
Pyroglyphidae/imunologia
Receptores de IgE/imunologia
[Mh] Termos MeSH secundário: Animais
Asma/patologia
Seres Humanos
[Pt] Tipo de publicação:EDITORIAL
[Nm] Nome de substância:
0 (Allergens); 0 (FCER1A protein, human); 0 (Receptors, IgE); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1165/rcmb.2017-0271ED


  5 / 1670 MEDLINE  
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[PMID]:29036225
[Au] Autor:Stroo I; Yang J; Anas AA; de Boer JD; van Mierlo G; Roem D; Wouters D; Engel R; Roelofs JJTH; van 't Veer C; van der Poll T; Zeerleder S
[Ad] Endereço:Department of Immunopathology, Sanquin Research, Amsterdam, the Netherlands.
[Ti] Título:Human plasma-derived C1 esterase inhibitor concentrate has limited effect on house dust mite-induced allergic lung inflammation in mice.
[So] Source:PLoS One;12(10):e0186652, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:C1 esterase inhibitor (C1-INH) can inhibit multiple pathways (complement, contact-kinin, coagulation, and fibrinolysis) that are all implicated in the pathophysiology of asthma. We explored the effect of human plasma-derived C1-INH on allergic lung inflammation in a house dust mite (HDM) induced asthma mouse model by daily administration of C1-INH (15 U) during the challenge phase. NaCl and HDM exposed mice had comparable plasma C1-INH levels, while bronchoalveolar lavage fluid (BALF) levels were increased in HDM exposed mice coinciding with slightly reduced activation of complement (C5a). C1-INH treatment reduced Th2 response and enhanced HDM-specific IgG1. Influx of eosinophils in BALF or lung, pulmonary damage, mucus production, procoagulant response or plasma leakage in BALF was similar in both groups. In conclusion, C1-INH dampens Th2 responses during HDM induced allergic lung inflammation.
[Mh] Termos MeSH primário: Asma/tratamento farmacológico
Asma/imunologia
Proteína Inibidora do Complemento C1/farmacologia
Pyroglyphidae/imunologia
[Mh] Termos MeSH secundário: Animais
Proteína Inibidora do Complemento C1/uso terapêutico
Feminino
Seres Humanos
Imunoglobulina G/imunologia
Camundongos
Células Th2/efeitos dos fármacos
Células Th2/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complement C1 Inhibitor Protein); 0 (Immunoglobulin G)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186652


  6 / 1670 MEDLINE  
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[PMID]:29026003
[Au] Autor:Chenuet P; Fauconnier L; Madouri F; Marchiol T; Rouxel N; Ledru A; Mauny P; Lory R; Uttenhove C; van Snick J; Iwakura Y; di Padova F; Quesniaux V; Togbe D; Ryffel B
[Ad] Endereço:Laboratory of Experimental and Molecular Immunology and Neurogenetics (INEM), UMR 7355 CNRS-University of Orleans, 3B, F-45071 Orleans-Cedex2, France.
[Ti] Título:Neutralization of either IL-17A or IL-17F is sufficient to inhibit house dust mite induced allergic asthma in mice.
[So] Source:Clin Sci (Lond);131(20):2533-2548, 2017 Oct 15.
[Is] ISSN:1470-8736
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:T helper (Th)17 immune response participates in allergic lung inflammation and asthma is reduced in the absence of interleukin (IL)-17 in mice. Since IL-17A and IL-17F are induced and bind the shared receptor IL-17RA, we asked whether both IL-17A and IL-17F contribute to house dust mite (HDM) induced asthma. We report that allergic lung inflammation is attenuated in absence of either IL-17A or IL-17F with reduced airway hyperreactivity, eosinophilic inflammation, goblet cell hyperplasia, cytokine and chemokine production as found in absence of IL-17RA. Furthermore, specific antibody neutralization of either IL-17A or IL-17F given during the sensitization phase attenuated allergic lung inflammation and airway hyperreactivity. activation by HDM of primary dendritic cells revealed a comparable induction of CXCL1 and IL-6 expression and the response to IL-17A and IL-17F relied on IL-17RA signaling via the adaptor protein act1 in fibroblasts. Therefore, HDM-induced allergic respiratory response depends on IL-17RA via act1 signaling and inactivation of either IL-17A or IL-17F is sufficient to attenuate allergic asthma in mice.
[Mh] Termos MeSH primário: Asma/tratamento farmacológico
Interleucina-17/antagonistas & inibidores
Pyroglyphidae/imunologia
[Mh] Termos MeSH secundário: Alérgenos/imunologia
Animais
Asma/imunologia
Células Dendríticas/imunologia
Modelos Animais de Doenças
Interleucina-17/imunologia
Interleucina-6/imunologia
Pulmão/imunologia
Camundongos Endogâmicos C57BL
Células Th17/imunologia
Células Th2/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Il17a protein, mouse); 0 (Il17f protein, mouse); 0 (Interleukin-17); 0 (Interleukin-6)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE
[do] DOI:10.1042/CS20171034


  7 / 1670 MEDLINE  
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[PMID]:28982170
[Au] Autor:Bordas-Le Floch V; Le Mignon M; Bussières L; Jain K; Martelet A; Baron-Bodo V; Nony E; Mascarell L; Moingeon P
[Ad] Endereço:Stallergenes Greer, Antony cedex, France.
[Ti] Título:A combined transcriptome and proteome analysis extends the allergome of house dust mite Dermatophagoides species.
[So] Source:PLoS One;12(10):e0185830, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: House dust mites (HDMs) such as Dermatophagoides farinae and D. pteronyssinus represent major causes of perennial allergy. HDM proteomes are currently poorly characterized, with information mostly restricted to allergens. As of today, 33 distinct allergen groups have been identified for these 2 mite species, with groups 1 and 2 established as major allergens. Given the multiplicity of IgE-reactive mite proteins, potential additional allergens have likely been overlooked. OBJECTIVE: To perform a comprehensive characterization of the transcriptomes, proteomes and allergomes of D. farinae and D. pteronyssinus in order to identify novel allergens. METHODS: Transcriptomes were analyzed by RNA sequencing and de novo assembly. Comprehensive mass spectrometry-based analyses proteomes were combined with two-dimensional IgE reactivity profiling. RESULTS: Transcripts from D. farinae and D. pteronyssinus were assembled, translated into protein sequences and used to populate derived sequence databases in order to inform immunoproteomic analyses. A total of 527 and 157 proteins were identified by bottom-up MS analyses in aqueous extracts from purified HDM bodies and fecal pellets, respectively. Based on high sequence similarities (>71% identity), we also identified 2 partial and 11 complete putative sequences of currently undisclosed D. pteronyssinus counterparts of D. farinae registered allergens. Immunoprofiling on 2D-gels revealed the presence of unknown 23 kDa IgE reactive proteins in both species. Following expression of non-glycosylated recombinant forms of these molecules, we confirm that these new allergens react with serum IgEs from 42% (8/19) of HDM-allergic individuals. CONCLUSIONS: Using combined transcriptome and immunoproteome approaches, we provide a comprehensive characterization of D. farinae and D. pteronyssinus allergomes. We expanded the known allergen repertoire for D. pteronyssinus and identified two novel HDM allergens, now officially referred by the International Union of Immunological Societies (IUIS) Nomenclature Subcommittee as Der f 36 and Der p 36.
[Mh] Termos MeSH primário: Alérgenos/metabolismo
Proteoma
Pyroglyphidae/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Alérgenos/química
Alérgenos/genética
Sequência de Aminoácidos
Animais
Hipersensibilidade/sangue
Espectrometria de Massas
Pyroglyphidae/genética
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Proteome)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185830


  8 / 1670 MEDLINE  
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[PMID]:28950285
[Au] Autor:Kiykim A; Mumcu G; Ogulur I; Karakoc-Aydiner E; Direskeneli H; Baris S; Cagan H; Ozen A
[Ad] Endereço:Department of Pediatric Allergy and Immunology, School of Medicine, Marmara University, Istanbul, Turkey.
[Ti] Título:Could Sublingual Immunotherapy Affect Oral Health in Children with Asthma and/or Allergic Rhinitis Sensitized to House Dust Mite?
[So] Source:Int Arch Allergy Immunol;174(1):52-56, 2017.
[Is] ISSN:1423-0097
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Sublingual immunotherapy (SLIT) has been successfully employed in IgE-mediated respiratory allergies. However, it is not known whether the modulation of immune responses in the sublingual area during SLIT has any deleterious effect on oral health. We sought to determine the oral health prospectively in children receiving SLIT for house dust mite allergy. MATERIAL AND METHODS: Eighteen children with allergic asthma and/or rhinitis and 31 age-matched healthy controls (HC) were included in an open-labeled trial. Oral health was evaluated by scoring the decayed, missing, and filled teeth for primary (dmft) and permanent (DMFT) dentition, and the plaque and gingival indices. Moreover, cariogenic food intake and teeth-brushing habits were also noted at baseline and at 19 months. RESULTS: The mean age of the SLIT participants was 9.5 ± 3.1 years and that of the HC was 9.2 ± 3.7 years. The mean duration of SLIT was 19.13 ± 3.81 months. At baseline, the total dmft and DMFT indices were similar in the SLIT and HC groups (p > 0.05), which demonstrated poor hygiene overall. In the within-group comparisons at the examination at 19 months, the SLIT group had a lower number of carious primary teeth and a higher number of filled primary teeth compared to the count at baseline (p = 0.027 and p = 0.058, respectively). CONCLUSION: Our study showed no detrimental effect of SLIT on oral health during a period of 19 months of follow-up. Parents should be motivated to use dental health services to prevent new caries formation since our cohort had overall poor oral hygiene at the baseline.
[Mh] Termos MeSH primário: Alérgenos/administração & dosagem
Antígenos de Dermatophagoides/administração & dosagem
Asma/imunologia
Saúde Bucal
Rinite Alérgica/imunologia
Imunoterapia Sublingual/efeitos adversos
Imunoterapia Sublingual/métodos
[Mh] Termos MeSH secundário: Administração Sublingual
Alérgenos/imunologia
Animais
Antígenos de Dermatophagoides/imunologia
Criança
Feminino
Seres Humanos
Masculino
Pyroglyphidae/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Antigens, Dermatophagoides)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1159/000480082


  9 / 1670 MEDLINE  
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[PMID]:28950271
[Au] Autor:Ohashi-Doi K; Kito H; Du W; Nakazawa H; Ipsen H; Gudmann P; Lund K
[Ad] Endereço:Torii Pharmaceutical Co. Ltd., Tokyo, Japan.
[Ti] Título:Bioavailability of House Dust Mite Allergens in Sublingual Allergy Tablets Is Highly Dependent on the Formulation.
[So] Source:Int Arch Allergy Immunol;174(1):26-34, 2017.
[Is] ISSN:1423-0097
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In sublingual immunotherapy (SLIT), the immune system is addressed by solubilized allergen that interacts with immunocompetent cells of the oral mucosa, the efficiency of which is governed by 2 main factors of SLIT allergen bioavailability: the allergen concentration and the mucosal contact time. Recently, 3 house dust mite (HDM) SLIT tablets were developed that differ with regard to allergen content, nominal strength (maintenance doses: 6 SQ-HDM/10,000 Japanese Allergen Units [JAU], 12 SQ-HDM/ 20,000 JAU, and 300 IR/57,000 JAU), and formulation (freeze-dried/compressed). Here, the importance of the SLIT tablet formulation for HDM major allergen bioavailability is examined. METHODS: The HDM major allergen content, tablet disintegration times, and allergen release kinetics were determined. Dissolution kinetics (allergen concentration vs. time) of Der f 1, Der p 1, and Der 2 were measured. Area under the curve (AUC) was used as a surrogate parameter for allergen bioavailability. RESULTS: The release of HDM major allergens from the freeze-dried tablets was complete after 30 s, while only partial release was achieved with the compressed tablets, even after prolonged dissolution. At 1 min, i.e., the recommended sublingual holding time for the freeze-dried tablets, the allergen bioavailability (AUC) of the compressed 300 IR/57,000 JAU tablet was 4.7-fold (Der f 1), 10.8-fold (Der p 1), and 23.6-fold (Der 2) lower than that of the freeze-dried 12 SQ-HDM/20,000 JAU tablet and similar to (Der f 1) and 5.3-fold (Der p 1) and 12.5-fold (Der 2) lower than that of the freeze-dried 6 SQ-HDM/10,000 JAU tablet. CONCLUSIONS: SLIT tablet allergen bioavailability depends highly on the tablet formulation. Only the fast-dissolving freeze-dried tablets provide maximal delivery of soluble allergens and achieve allergen concentrations that reflect the nominal tablet strengths within the recommended sublingual holding time.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/farmacocinética
Cisteína Endopeptidases/farmacocinética
Imunoterapia Sublingual/métodos
[Mh] Termos MeSH secundário: Administração Sublingual
Animais
Antígenos de Dermatophagoides/imunologia
Proteínas de Artrópodes/imunologia
Disponibilidade Biológica
Cisteína Endopeptidases/imunologia
Seres Humanos
Pyroglyphidae/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Dermatophagoides); 0 (Arthropod Proteins); 0 (Dermatophagoides farinae antigen f 2); EC 3.4.22.- (Cysteine Endopeptidases); EC 3.4.22.- (Dermatophagoides farinae antigen f 1); EC 3.4.22.- (Dermatophagoides pteronyssinus antigen p 1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1159/000479693


  10 / 1670 MEDLINE  
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[PMID]:28863172
[Au] Autor:Huff RD; Hsu AC; Nichol KS; Jones B; Knight DA; Wark PAB; Hansbro PM; Hirota JA
[Ad] Endereço:Division of Respiratory Medicine, Department of Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
[Ti] Título:Regulation of xanthine dehydrogensase gene expression and uric acid production in human airway epithelial cells.
[So] Source:PLoS One;12(9):e0184260, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: The airway epithelium is a physical and immunological barrier that protects the pulmonary system from inhaled environmental insults. Uric acid has been detected in the respiratory tract and can function as an antioxidant or damage associated molecular pattern. We have demonstrated that human airway epithelial cells are a source of uric acid. Our hypothesis is that uric acid production by airway epithelial cells is induced by environmental stimuli associated with chronic respiratory diseases. We therefore examined how airway epithelial cells regulate uric acid production. MATERIALS AND METHODS: Allergen and cigarette smoke mouse models were performed using house dust mite (HDM) and cigarette smoke exposure, respectively, with outcome measurements of lung uric acid levels. Primary human airway epithelial cells isolated from clinically diagnosed patients with asthma and chronic obstructive pulmonary disease (COPD) were grown in submerged cultures and compared to age-matched healthy controls for uric acid release. HBEC-6KT cells, a human airway epithelial cell line, were grown under submerged monolayer conditions for mechanistic and gene expression studies. RESULTS: HDM, but not cigarette smoke exposure, stimulated uric acid production in vivo and in vitro. Primary human airway epithelial cells from asthma, but not COPD patients, displayed elevated levels of extracellular uric acid in culture. In HBEC-6KT, production of uric acid was sensitive to the xanthine dehydrogenase (XDH) inhibitor, allopurinol, and the ATP Binding Cassette C4 (ABCC4) inhibitor, MK-571. Lastly, the pro-inflammatory cytokine combination of TNF-α and IFN-γ elevated extracellular uric acid levels and XDH gene expression in HBEC-6KT cells. CONCLUSIONS: Our results suggest that the active production of uric acid from human airway epithelial cells may be intrinsically altered in asthma and be further induced by pro-inflammatory cytokines.
[Mh] Termos MeSH primário: Células Epiteliais/metabolismo
Regulação Enzimológica da Expressão Gênica
Ácido Úrico/metabolismo
Xantina Desidrogenase/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Alérgenos
Animais
Asma/metabolismo
Brônquios/citologia
Brônquios/efeitos dos fármacos
Células Cultivadas
Modelos Animais de Doenças
Células Epiteliais/efeitos dos fármacos
Feminino
Perfilação da Expressão Gênica
Seres Humanos
Interferon gama/farmacologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Meia-Idade
Doença Pulmonar Obstrutiva Crônica/metabolismo
Pyroglyphidae
Fumaça
Fumar
Produtos do Tabaco
Fator de Necrose Tumoral alfa/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Smoke); 0 (Tumor Necrosis Factor-alpha); 268B43MJ25 (Uric Acid); 82115-62-6 (Interferon-gamma); EC 1.17.1.4 (Xanthine Dehydrogenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170902
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184260



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