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Pesquisa : B01.050.500.131.365 [Categoria DeCS]
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[PMID]:28457892
[Au] Autor:Chen CC; Wang YH; Wang SL; Huang PC; Chuang SC; Chen MH; Chen BH; Sun CW; Fu HC; Lee CC; Wu MT; Chen ML; Hsiung CA
[Ad] Endereço:Institute of Population Health Sciences, National Health Research Institutes, Miaoli, Taiwan. Electronic address: ccchen@nhri.org.tw.
[Ti] Título:Exposure sources and their relative contributions to urinary phthalate metabolites among children in Taiwan.
[So] Source:Int J Hyg Environ Health;220(5):869-879, 2017 07.
[Is] ISSN:1618-131X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Phthalate exposure is omnipresent and known to have developmental and reproductive effects in children. The aim of this study was to determine the phthalate exposure sources and their relative contributions among children in Taiwan. During the first wave of the Risk Assessment of Phthalate Incident in Taiwan (RAPIT), in 2012, we measured 8 urinary phthalate metabolites in 226 children aged 1-11 years old and in 181 children from the same cohort for the wave 2 study in 2014. A two-stage statistical analysis approach was adopted. First, a stepwise regression model was used to screen 80 questions that explored the exposure frequency and lifestyle for potential associations. Second, the remaining questions with positive regression coefficients were grouped into the following 6 exposure categories: plastic container/packaging, food, indoor environment, personal care products, toys, and eating out. A mixed model was then applied to assess the relative contributions of these categories for each metabolite. The use of plastic container or food packaging were dominant exposure sources for mono-2-ethylhexyl phthalate (MEHP), mono-2-ethyl-5-hydroxyhexyl phthalate (MEHHP), mono-2-ethyl-5-oxohexyl phthalate (MEOHP), and mono-n-butyl phthalate (MnBP). The indoor environment was a major exposure source of mono-methyl phthalate (MMP), mono-benzyl phthalate (MBzP), and mono-isobutyl phthalate (MiBP). The consumption of seafood showed a significant correlation with MEHP. The children's modified dietary behavior and improved living environment in the second study wave were associated with lower phthalate metabolite levels, showing that phthalate exposures can be effectively reduced.
[Mh] Termos MeSH primário: Exposição Ambiental/análise
Poluentes Ambientais/urina
Ácidos Ftálicos/urina
Plastificantes/análise
[Mh] Termos MeSH secundário: Animais
Criança
Pré-Escolar
Crustáceos
Dieta
Feminino
Peixes
Contaminação de Alimentos
Embalagem de Alimentos
Seres Humanos
Lactente
Masculino
Alimentos Marinhos
Inquéritos e Questionários
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Environmental Pollutants); 0 (Phthalic Acids); 0 (Plasticizers)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:28460028
[Au] Autor:Eyun SI; Soh HY; Posavi M; Munro JB; Hughes DST; Murali SC; Qu J; Dugan S; Lee SL; Chao H; Dinh H; Han Y; Doddapaneni H; Worley KC; Muzny DM; Park EO; Silva JC; Gibbs RA; Richards S; Lee CE
[Ad] Endereço:Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, NE.
[Ti] Título:Evolutionary History of Chemosensory-Related Gene Families across the Arthropoda.
[So] Source:Mol Biol Evol;34(8):1838-1862, 2017 Aug 01.
[Is] ISSN:1537-1719
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chemosensory-related gene (CRG) families have been studied extensively in insects, but their evolutionary history across the Arthropoda had remained relatively unexplored. Here, we address current hypotheses and prior conclusions on CRG family evolution using a more comprehensive data set. In particular, odorant receptors were hypothesized to have proliferated during terrestrial colonization by insects (hexapods), but their association with other pancrustacean clades and with independent terrestrial colonizations in other arthropod subphyla have been unclear. We also examine hypotheses on which arthropod CRG family is most ancient. Thus, we reconstructed phylogenies of CRGs, including those from new arthropod genomes and transcriptomes, and mapped CRG gains and losses across arthropod lineages. Our analysis was strengthened by including crustaceans, especially copepods, which reside outside the hexapod/branchiopod clade within the subphylum Pancrustacea. We generated the first high-resolution genome sequence of the copepod Eurytemora affinis and annotated its CRGs. We found odorant receptors and odorant binding proteins present only in hexapods (insects) and absent from all other arthropod lineages, indicating that they are not universal adaptations to land. Gustatory receptors likely represent the oldest chemosensory receptors among CRGs, dating back to the Placozoa. We also clarified and confirmed the evolutionary history of antennal ionotropic receptors across the Arthropoda. All antennal ionotropic receptors in E. affinis were expressed more highly in males than in females, suggestive of an association with male mate-recognition behavior. This study is the most comprehensive comparative analysis to date of CRG family evolution across the largest and most speciose metazoan phylum Arthropoda.
[Mh] Termos MeSH primário: Artrópodes/genética
Receptores Odorantes/genética
[Mh] Termos MeSH secundário: Animais
Células Quimiorreceptoras/fisiologia
Copépodes/genética
Crustáceos/genética
Bases de Dados de Ácidos Nucleicos
Evolução Molecular
Genoma/genética
Insetos/genética
Família Multigênica/genética
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Odorant); 0 (odorant-binding protein)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1093/molbev/msx147


  3 / 6619 MEDLINE  
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[PMID]:28947316
[Au] Autor:Min BH; Ravikumar Y; Lee DH; Choi KS; Kim BM; Rhee JS
[Ad] Endereço:Aquaculture Research Division, National Institute of Fisheries Science, Busan 46083, South Korea.
[Ti] Título:Age-dependent antioxidant responses to the bioconcentration of microcystin-LR in the mysid crustacean, Neomysis awatschensis.
[So] Source:Environ Pollut;232:284-292, 2018 Jan.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microcystins (MCs) are naturally occurring algal toxins in the aquatic environment and pose a serious threat to the ecosystem. In general, aquatic populations are structured by organisms of different ages, with varying degrees of biochemical and physiological responses. In this study, juvenile and adult marine mysids (Neomysis awatschensis) were exposed to MC-Leucine Arginine (MC-LR) (0.1, 1, and 10 µg L ) for 7 days, and the bioconcentration dynamics and responses of antioxidant defense system were measured during the exposure and additional depuration periods (7 days). MC-LR bioconcentrated in a dose-dependent manner, from a threshold concentration of 1 µg L in both stages, and the levels reduced gradually during the depuration phase. Bioconcentration patterns of MC-LR were highly age-specific, as juvenile mysids showed peaks during the exposure period, whereas adults exhibited a peak on the first day of depuration. After exposure to 10 µg L concentration, elevated levels of malondialdehyde (MDA) and glutathione (GSH) were observed during the late (days 5 and 7) exposure and early (days 1 and 3) depuration periods in juvenile mysids, while adult mysids showed a peak on day 7 of the exposure period. Age-specific responses were also observed in the enzymatic activities of glutathione S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR). Juvenile mysids showed a significant elevation in all enzymatic activities during the exposure and/or depuration phase upon exposure to 10 µg L MC-LR, but only CAT and SOD enzymes showed significant changes during the exposure and/or depuration periods in adults. Overall, our results indicate the bioconcentration potential of MC-LR and its threshold in the marine mysid, in addition to age-specific MC-LR dynamics and subsequent biochemical responses.
[Mh] Termos MeSH primário: Crustáceos/fisiologia
Microcistinas/metabolismo
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Animais
Antioxidantes
Catalase/metabolismo
Glutationa/metabolismo
Glutationa Peroxidase/metabolismo
Glutationa Redutase
Glutationa Transferase/metabolismo
Malondialdeído
Microcistinas/toxicidade
Estresse Oxidativo
Superóxido Dismutase/metabolismo
Poluentes Químicos da Água/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Microcystins); 0 (Water Pollutants, Chemical); 4Y8F71G49Q (Malondialdehyde); 77238-39-2 (microcystin); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); EC 1.8.1.7 (Glutathione Reductase); EC 2.5.1.18 (Glutathione Transferase); EQ8332842Y (cyanoginosin LR); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180119
[Lr] Data última revisão:
180119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE


  4 / 6619 MEDLINE  
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[PMID]:29236717
[Au] Autor:Das S; Tseng LC; Wang L; Hwang JS
[Ad] Endereço:Institute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan.
[Ti] Título:Burrow characteristics of the mud shrimp Austinogebia edulis, an ecological engineer causing sediment modification of a tidal flat.
[So] Source:PLoS One;12(12):e0187647, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mud shrimp Austinogebia edulis, being abundant in the intertidal zone of western Taiwan, constructs deep burrows (>1 m). This study highlights the potential of mud shrimps to modify sediment characteristics of the tidal flat by its burrowing behavior. We studied the structure of the burrow wall, compared the difference in the sediment composition of the burrow and the background sediment, and compared the organic content inside the burrow wall. This study was carried out from September 2015 to November 2016 in three areas of the western coast of Taiwan, namely Shengang, Hanbow, and Wangong. The present study found significant differences between burrow wall and the burrow lumen. The diameter of the burrow wall was double as wide as the inner burrow lumen at the opening and gradually increased to 10 times of the burrow lumen at 30 cm depth. The burrow wall of A. edulis showed low permeability and increased the sheer strength. Statistically, a significant difference was noticed in the comparison between the sediment composition of the burrow wall and the background (p < 0.05, Student's t-test). An accumulation of 3.63 for fine sand (t = -5.22, p < 0.001, fine sand) and 9 for clay (t = -25.01, p < 0.001, clay) was found in the upper burrow wall of A. edulis. This indicated that they somehow chose finer particles to build burrows. This will gradually change the sediment distribution-vertically and horizontally. The burrow wall consisted of a 24 times higher organic matter content than one individual of mud shrimp. The burrow may provide organic material as a potential food source. The mud shrimp thus transforms the sediment characteristics as an ecological engineer, which is expected to have a significant ecological impact on the ecosystem.
[Mh] Termos MeSH primário: Comportamento Animal
Crustáceos/fisiologia
Ecossistema
[Mh] Termos MeSH secundário: Animais
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187647


  5 / 6619 MEDLINE  
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[PMID]:29244845
[Au] Autor:Merten V; Christiansen B; Javidpour J; Piatkowski U; Puebla O; Gasca R; Hoving HT
[Ad] Endereço:GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany.
[Ti] Título:Diet and stable isotope analyses reveal the feeding ecology of the orangeback squid Sthenoteuthis pteropus (Steenstrup 1855) (Mollusca, Ommastrephidae) in the eastern tropical Atlantic.
[So] Source:PLoS One;12(12):e0189691, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the eastern tropical Atlantic, the orangeback flying squid Sthenoteuthis pteropus (Steenstrup 1855) (Cephalopoda, Ommastrephidae) is a dominant species of the epipelagic nekton community. This carnivore squid has a short lifespan and is one of the fastest-growing squids. In this study, we characterise the role of S. pteropus in the pelagic food web of the eastern tropical Atlantic by investigating its diet and the dynamics of its feeding habits throughout its ontogeny and migration. During three expeditions in the eastern tropical Atlantic in 2015, 129 specimens were caught by hand jigging. Stomach content analyses (via visual identification and DNA barcoding) were combined with stable isotope data (∂15N and ∂13C) of muscle tissue to describe diet, feeding habits and trophic ecology of S. pteropus. Additionally, stable isotope analyses of incremental samples along the squid's gladius-the chitinous spiniform structure supporting the muscles and organs-were carried out to explore possible diet shifts through ontogeny and migration. Our results show that S. pteropus preys mainly on myctophid fishes (e.g. Myctophum asperum, Myctophum nitidulum, Vinciguerria spp.), but also on other teleost species, cephalopods (e.g. Enoploteuthidae, Bolitinidae, Ommastrephidae), crustaceans and possibly on gelatinous zooplankton as well. The squid shows a highly opportunistic feeding behaviour that includes cannibalism. Our study indicates that the trophic position of S. pteropus may increase by approximately one trophic level from a mantle length of 15 cm to 47 cm. The reconstructed isotope-based feeding chronologies of the gladii revealed high intra- and inter-individual variability in the squid's trophic position and foraging area. These findings are not revealed by diet or muscle tissue stable isotope analysis. This suggests a variable and complex life history involving individual variation and migration. The role of S. pteropus in transferring energy and nutrients from lower to higher trophic levels may be underestimated and important for understanding how a changing ocean impacts food webs in the eastern Atlantic.
[Mh] Termos MeSH primário: Decapodiformes/fisiologia
Ecologia
Comportamento Alimentar
[Mh] Termos MeSH secundário: Animais
Isótopos de Carbono
Crustáceos
Código de Barras de DNA Taxonômico
Peixes
Conteúdo Gastrointestinal/química
Isótopos de Nitrogênio
Estado Nutricional
Alimentos Marinhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbon Isotopes); 0 (Nitrogen Isotopes)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189691


  6 / 6619 MEDLINE  
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[PMID]:28982619
[Au] Autor:Liu Q; Xu D; Jiang S; Huang J; Zhou F; Yang Q; Jiang S; Yang L
[Ad] Endereço:South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; College of Aqua-life Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of
[Ti] Título:Toll-receptor 9 gene in the black tiger shrimp (Penaeus monodon) induced the activation of the TLR-NF-κB signaling pathway.
[So] Source:Gene;639:27-33, 2018 Jan 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Toll receptors are important pathogen recognition receptors (PRRs) in shrimps, which play a vital role in defending against virus and bacterial challenge. In this paper, the characterization and functional analysis of a Toll9 receptor gene from Penaeus monodon was performed in HEK293T cells. Data showed that PmToll9 can activate the NF-κB promoter activities of TLR pathway, while ISRE and IFN-ß promoter cannot be activated obviously in HEK293T cells using dual-luciferase reporter system. The downstream immune factors of IL-8, IκB-α, and TRAF6 were activated by PmToll9 and IL-8 showed the most significant up-regulation in expression levels, indicating the activities of NF-κB can be mediated by PmToll9. Six LRRs-deletion mutants were constructed and results showed these mutants had obvious declines in luciferase activities, among which the mutant pCMV-DeLRR4 showed the most significant decline. qPCR data indicated LRRs-deletion mutants efficiently impaired the activities of the downstream immune factors IL-8, IκB-α, and TRAF6. It demonstrates that LRRs-deletion mutants could result in the weaken abilities of PmToll9 in signaling transduction. Overexpression of PmToll9-GFP fusion protein in Hela cells revealed the primary cellular localization of PmToll9 is in the cytoplasm.
[Mh] Termos MeSH primário: NF-kappa B/metabolismo
Transdução de Sinais
Receptor Toll-Like 9/genética
[Mh] Termos MeSH secundário: Animais
Crustáceos
Células HEK293
Células HeLa
Seres Humanos
Receptor Toll-Like 9/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NF-kappa B); 0 (Toll-Like Receptor 9)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE


  7 / 6619 MEDLINE  
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[PMID]:29065166
[Au] Autor:Rumisha C; Huyghe F; Rapanoel D; Mascaux N; Kochzius M
[Ad] Endereço:Sokoine University of Agriculture, Solomon Mahlangu College of Science and Education, Department of Biosciences, Morogoro, Tanzania.
[Ti] Título:Genetic diversity and connectivity in the East African giant mud crab Scylla serrata: Implications for fisheries management.
[So] Source:PLoS One;12(10):e0186817, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The giant mud crab Scylla serrata provides an important source of income and food to coastal communities in East Africa. However, increasing demand and exploitation due to the growing coastal population, export trade, and tourism industry are threatening the sustainability of the wild stock of this species. Because effective management requires a clear understanding of the connectivity among populations, this study was conducted to assess the genetic diversity and connectivity in the East African mangrove crab S. serrata. A section of 535 base pairs of the cytochrome oxidase subunit I (COI) gene and eight microsatellite loci were analysed from 230 tissue samples of giant mud crabs collected from Kenya, Tanzania, Mozambique, Madagascar, and South Africa. Microsatellite genetic diversity (He) ranged between 0.56 and 0.6. The COI sequences showed 57 different haplotypes associated with low nucleotide diversity (current nucleotide diversity = 0.29%). In addition, the current nucleotide diversity was lower than the historical nucleotide diversity, indicating overexploitation or historical bottlenecks in the recent history of the studied population. Considering that the coastal population is growing rapidly, East African countries should promote sustainable fishing practices and sustainable use of mangrove resources to protect mud crabs and other marine fauna from the increasing pressure of exploitation. While microsatellite loci did not show significant genetic differentiation (p > 0.05), COI sequences revealed significant genetic divergence between sites on the East coast of Madagascar (ECM) and sites on the West coast of Madagascar, mainland East Africa, as well as the Seychelles. Since East African countries agreed to achieve the Convention on Biological Diversity (CBD) target to protect over 10% of their marine areas by 2020, the observed pattern of connectivity and the measured genetic diversity can serve to provide useful information for designing networks of marine protected areas.
[Mh] Termos MeSH primário: Conservação dos Recursos Naturais
Crustáceos/genética
Pesqueiros
[Mh] Termos MeSH secundário: África Oriental
Animais
Variação Genética
Repetições de Microssatélites/genética
Reação em Cadeia da Polimerase Multiplex
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171025
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186817


  8 / 6619 MEDLINE  
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[PMID]:28961269
[Au] Autor:Gharouni A; Barbeau MA; Chassé J; Wang L; Watmough J
[Ad] Endereço:Department of Mathematics and Statistics, University of New Brunswick, Fredericton, Canada.
[Ti] Título:Stochastic dispersal increases the rate of upstream spread: A case study with green crabs on the northwest Atlantic coast.
[So] Source:PLoS One;12(9):e0185671, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dispersal heterogeneity is an important process that can compensate for downstream advection, enabling aquatic organisms to persist or spread upstream. Our main focus was the effect of year-to-year variation in larval dispersal on invasion spread rate. We used the green crab, Carcinus maenas, as a case study. This species was first introduced over 200 years ago to the east coast of North America, and once established has maintained a relatively consistent spread rate against the dominant current. We used a stage-structured, integro-difference equation model that couples a demographic matrix for population growth and dispersal kernels for spread of individuals within a season. The kernel describing larval dispersal, the main dispersive stage, was mechanistically modeled to include both drift and settlement rate components. It was parameterized using a 3-dimensional hydrodynamic model of the Gulf of St Lawrence, which enabled us to incorporate larval behavior, namely vertical swimming. Dispersal heterogeneity was modeled at two temporal scales: within the larval period (months) and over the adult lifespan (years). The kernel models variation within the larval period. To model the variation among years, we allowed the kernel parameters to vary by year. Results indicated that when dispersal parameters vary with time, knowledge of the time-averaged dispersal process is insufficient for determining the upstream spread rate of the population. Rather upstream spread is possible over a number of years when incorporating the yearly variation, even when there are only a few "good years" featured by some upstream dispersal among many "bad years" featured by only downstream dispersal. Accounting for annual variations in dispersal in population models is important to enhance understanding of spatial dynamics and population spread rates. Our developed model also provides a good platform to link the modeling of larval behavior and demography to large-scale hydrodynamic models.
[Mh] Termos MeSH primário: Crustáceos
Processos Estocásticos
[Mh] Termos MeSH secundário: Animais
Oceano Atlântico
Demografia
Noroeste dos Estados Unidos
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170930
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185671


  9 / 6619 MEDLINE  
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[PMID]:28931061
[Au] Autor:Sun JJ; Lan JF; Zhao XF; Vasta GR; Wang JX
[Ad] Endereço:Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, China.
[Ti] Título:Binding of a C-type lectin's coiled-coil domain to the Domeless receptor directly activates the JAK/STAT pathway in the shrimp immune response to bacterial infection.
[So] Source:PLoS Pathog;13(9):e1006626, 2017 Sep.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:C-type lectins (CTLs) are characterized by the presence of a C-type carbohydrate recognition domain (CTLD) that by recognizing microbial glycans, is responsible for their roles as pattern recognition receptors in the immune response to bacterial infection. In addition to the CTLD, however, some CTLs display additional domains that can carry out effector functions, such as the collagenous domain of the mannose-binding lectin. While in vertebrates, the mechanisms involved in these effector functions have been characterized in considerable detail, in invertebrates they remain poorly understood. In this study, we identified in the kuruma shrimp (Marsupenaeus japonicus) a structurally novel CTL (MjCC-CL) that in addition to the canonical CTLD, contains a coiled-coil domain (CCD) responsible for the effector functions that are key to the shrimp's antibacterial response mediated by antimicrobial peptides (AMPs). By the use of in vitro and in vivo experimental approaches we elucidated the mechanism by which the recognition of bacterial glycans by the CTLD of MjCC-CL leads to activation of the JAK/STAT pathway via interaction of the CCD with the surface receptor Domeless, and upregulation of AMP expression. Thus, our study of the shrimp MjCC-CL revealed a striking functional difference with vertebrates, in which the JAK/STAT pathway is indirectly activated by cell death and stress signals through cytokines or growth factors. Instead, by cross-linking microbial pathogens with the cell surface receptor Domeless, a lectin directly activates the JAK/STAT pathway, which plays a central role in the shrimp antibacterial immune responses by upregulating expression of selected AMPs.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/metabolismo
Crustáceos/metabolismo
Lectinas Tipo C/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Proteínas de Artrópodes/imunologia
Crustáceos/microbiologia
DNA Complementar/genética
Janus Quinases/metabolismo
Lectinas Tipo C/química
Receptores de Reconhecimento de Padrão/metabolismo
Fatores de Transcrição STAT/metabolismo
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (DNA, Complementary); 0 (Lectins, C-Type); 0 (Receptors, Pattern Recognition); 0 (STAT Transcription Factors); EC 2.7.10.2 (Janus Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006626


  10 / 6619 MEDLINE  
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[PMID]:28886062
[Au] Autor:Sacristán HJ; Rodríguez YE; De Los Angeles Pereira N; López Greco LS; Lovrich GA; Fernández Gimenez AV
[Ad] Endereço:Centro Austral de Investigaciones Científicas, CONICET, Houssay, Ushuaia, Argentina.
[Ti] Título:Energy reserves mobilization: Strategies of three decapod species.
[So] Source:PLoS One;12(9):e0184060, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In food deprivation assays, several different responses have been observed in crustaceans. However, studying energy reserves utilization among more than one species during the same starvation period has not yet been performed, particularly to discern whether the responses are due to intrinsic and/or environmental factors. We hypothesize that decapod species with similar feeding habits have the same strategies in the use of energetic reserves during starvation, even though they inhabit different environments. The aim of this study was to compare the energy reserves mobilization of three decapods species (Cherax quadricarinatus, Palaemon argentinus and Munida gregaria) with similar feeding habits, exposed to similar food deprivation conditions. The crayfish, shrimp and squat-lobster were experimentally kept at continuous feeding or continuous starvation throughout 15 days. Every 3rd day, the midgut gland index (MGI), and the glycogen, lipid and protein contents were measured in the midgut gland (MG) and pleon muscle. Palaemon argentinus mobilized more reserves during starvation, followed by C. quadricarinatus, and the last M. gregaria. The starved shrimps presented low MGI, whereas MG showed a reduction in glycogen (from day 6 to 15), lipid (from day 3 to 15), and protein levels (at day 9 and 15) while in their muscle, lipid reserves decreased at days 3 and 6. In C. quadricarinatus, the most affected parameters in the MG were MGI, glycogen (from day 6 to 15), and lipids (at day 12 and 15). In the MG of M. gregaria only the glycogen was reduced during fasting from 3 to 15 days. Even though the three studied species have similar feeding habitats, we found that their energetic profile utilization is different and it could be explained by the habitat, life span, temperature, organ/tissue, and metabolism of the species. Our results may be useful to understand the several different responses of crustaceans during starvation.
[Mh] Termos MeSH primário: Crustáceos/fisiologia
Metabolismo Energético
Privação de Alimentos
[Mh] Termos MeSH secundário: Animais
Astacoidea/fisiologia
Crustáceos/classificação
Meio Ambiente
Glicogênio/metabolismo
Metabolismo dos Lipídeos
Músculos/metabolismo
Filogenia
Inanição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9005-79-2 (Glycogen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184060



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