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  1 / 3851 MEDLINE  
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[PMID]:29228938
[Au] Autor:Nishijima S; Nishikawa C; Miyashita T
[Ad] Endereço:Laboratory of Biodiversity Science, School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, 113-8657, Japan. nishijimash@gmail.com.
[Ti] Título:Habitat modification by invasive crayfish can facilitate its growth through enhanced food accessibility.
[So] Source:BMC Ecol;17(1):37, 2017 Dec 12.
[Is] ISSN:1472-6785
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Invasive ecosystem engineers can facilitate their invasions by modifying the physical environment to improve their own performance, but this positive feedback process has rarely been tested empirically except in sessile organisms. The invasive crayfish Procambarus clarkii is an ecosystem engineer that destroys aquatic macrophytes, which provide a physical refuge for animal prey, and this destruction is likely to enhance vulnerability to predators. Using two series of mesocosm experiments, we tested the hypothesis that the invasive crayfish increases its feeding efficiency on animal prey by reducing submerged macrophytes, thus increasing its individual growth rate in a positive density-dependent manner. RESULTS: In the first experiment, increasing crayfish density reduced both macrophytes and animal prey (dragonfly and chironomid larvae) and, importantly, increased the growth rate of individual crayfish, in accordance with our expectation. In the second experiment, we used artificial macrophytes to clarify whether the physical architecture of macrophytes itself protects animal prey and limits crayfish growth rate. Increasing the artificial macrophyte quantity not only increased the survival of animal prey, but also retarded the crayfish growth rate. CONCLUSIONS: We conclude that macrophytes strengthen bottom-up control of crayfish, but this effect can be relaxed by increasing the density of crayfish via reduction in macrophytes. This positive feedback process may explain the crayfish outbreaks and regime shifts occasionally observed in invaded freshwater ecosystems.
[Mh] Termos MeSH primário: Astacoidea/fisiologia
Ecossistema
Cadeia Alimentar
Espécies Introduzidas
[Mh] Termos MeSH secundário: Animais
Astacoidea/crescimento & desenvolvimento
Chironomidae/crescimento & desenvolvimento
Comportamento Alimentar
Água Doce
Larva/crescimento & desenvolvimento
Odonatos/crescimento & desenvolvimento
Desenvolvimento Vegetal
Densidade Demográfica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1186/s12898-017-0147-7


  2 / 3851 MEDLINE  
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[PMID]:28966124
[Au] Autor:Gan HM; Tan MH; Lee YP; Schultz MB; Horwitz P; Burnham Q; Austin CM
[Ad] Endereço:Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3220, Australia; Genomics Facility, Tropical and Medicine Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Petaling Jaya, Selangor, Malaysia; School
[Ti] Título:More evolution underground: Accelerated mitochondrial substitution rate in Australian burrowing freshwater crayfishes (Decapoda: Parastacidae).
[So] Source:Mol Phylogenet Evol;118:88-98, 2018 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To further understand the evolutionary history and mitogenomic features of Australia's highly distinctive freshwater crayfish fauna, we utilized a recently described rapid mitogenome sequencing pipeline to generate 24 new crayfish mitogenomes including a diversity of burrowing crayfish species and the first for Astacopsis gouldi, the world's largest freshwater invertebrate. Whole mitogenome-based phylogeny estimates using both Bayesian and Maximum Likelihood methods substantially strengthen existing hypotheses for systematic relationships among Australian freshwater crayfish with evidence of pervasive diversifying selection and accelerated mitochondrial substitution rate among the members of the clade representing strongly burrowing crayfish that may reflect selection pressures for increased energy requirement for adaptation to terrestrial environment and a burrowing lifestyle. Further, gene rearrangements are prevalent in the burrowing crayfish mitogenomes involving both tRNA and protein coding genes. In addition, duplicated control regions were observed in two closely related Engaeus species, together with evidence for concerted evolution. This study significantly adds to the understanding of Australian freshwater crayfish evolutionary relationships and suggests a link between mitogenome evolution and adaptation to terrestrial environments and a burrowing lifestyle in freshwater crayfish.
[Mh] Termos MeSH primário: Astacoidea/classificação
DNA Mitocondrial/genética
Evolução Molecular
[Mh] Termos MeSH secundário: Animais
Astacoidea/genética
Austrália
Teorema de Bayes
Códon
DNA/química
DNA/isolamento & purificação
DNA/metabolismo
DNA Mitocondrial/química
DNA Mitocondrial/classificação
DNA Mitocondrial/metabolismo
Água Doce
Ordem dos Genes
Funções Verossimilhança
Filogenia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon); 0 (DNA, Mitochondrial); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE


  3 / 3851 MEDLINE  
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[PMID]:29089143
[Au] Autor:Tang X; Liang Q; Liu L; Sheng X; Xing J; Zhan W
[Ad] Endereço:Laboratory of Pathology and Immunology of Aquatic Animals, KLM, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No.1 Wenhai Road, Aoshanwei Town,
[Ti] Título:An optimized double-antibody sandwich ELISA for quantitative detection of WSSV in artificially infected crayfish.
[So] Source:J Virol Methods;251:133-138, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Developing a rapid, accurate and quantitative method for detecting white spot syndrome virus (WSSV) is extremely urgent and critical for reducing the risk of white spot disease outbreaks. In the present work, an optimized double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was developed for quantitative detection of WSSV. The method employed rabbit polyclonal antibodies against WSSV as the capture antibody and previously produced anti-WSSV monoclonal antibodies as the detector antibody. A standard curve of the log concentration of WSSV versus OD value was established, which was linear in the concentration range of 120-7680ng/mL, and the linear regression equation was y=0.166x-0.151. Viral proteins in different tissues of crayfish (Procambarus clarkia) post artificial infection with WSSV were quantitatively measured using the DAS-ELISA. WSSV proliferated quickly within 60h post infection and gradually slowed down afterwards. According to the linear regression relationship, the viral proteins in hemolymph, gut and gonad were firstly able to be quantified at 24h post infection with the concentrations of 186, 158 and 128ng/mL, respectively. These three tissues also contained higher viral proteins than the gill, heart, hepatopancreas and muscle during the entire infection period. The viral protein concentration in gut reached the highest level of 6220ng/mL at 72h post infection. Real time quantitative PCR was also used to detect the dynamic change of viral copies in crayfish hemolymph post WSSV infection, with similar results for both assays. The developed DAS-ELISA could detect WSSV propagation from initial to moribund stage in infected crayfish and demonstrated potential application for diagnosis of WSSV.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Astacoidea/virologia
Infecções por Vírus de DNA/veterinária
Ensaio de Imunoadsorção Enzimática/métodos
Carga Viral/métodos
Vírus 1 da Síndrome da Mancha Branca/isolamento & purificação
[Mh] Termos MeSH secundário: Estruturas Animais/virologia
Animais
Infecções por Vírus de DNA/virologia
Coelhos
Fatores de Tempo
Vírus 1 da Síndrome da Mancha Branca/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171102
[St] Status:MEDLINE


  4 / 3851 MEDLINE  
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[PMID]:29050792
[Au] Autor:Sakuna K; Elliman J; Owens L
[Ad] Endereço:College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, 4811, Australia; Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, 80240, Thailand.
[Ti] Título:Comparison of molecular detection PCR methods for chequa iflavirus in freshwater crayfish, Cherax quadricarinatus.
[So] Source:J Virol Methods;251:139-144, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chequa iflavirus (+ve sense ssRNA virus) infects redclaw crayfish (Cherax quadricarinatus) and it may cause mortality reaching 20-40% after about three weeks following stress. The sequence of the RNA-dependent RNA polymerase at nucleotide position 8383-9873 was used for developing and comparing PCR-based detection protocols. The reverse transcription, quantitative, polymerase chain reaction (RT-qPCR) was specific against nine Picornavirales and crustacean viruses and its' measurement of uncertainty (0.07-1.37) was similar to PCRs for other crustacean viruses. In vitro, the reverse transcription loop-mediated isothermal amplification (RT-LAMP) read at 60min had poor repeatability for a linearized plasmid with an iflavirus insert when compared with RT-PCR visualised on an electrophoretic gel and RT-qPCR; both sensitive to 10 copies. In a limited, comparative sample of clinical crayfish haemolymph, the lowest, non-zero copies were 2.88×10 for RT-PCR and 4.60×10 for the RT-qPCR. In 68 further clinical crayfish haemolymph samples tested by RT-qPCR only, copy numbers ranged from 0 to 1.14×10 . For RT-qPCR, the amplification plots, melt curves and the C values indicated that the C above 34.0 is a potential negative result but examination of the melt curve is necessary for an accurate interpretation. A suggested program of testing for crayfish farmers would consist of non-destructive bleeding, labelling of crayfish and screening with RT-qPCR. Only those crayfish nominally negative (below detectable limits) would be used for broodstock or selective breeding.
[Mh] Termos MeSH primário: Astacoidea/virologia
Técnicas de Diagnóstico Molecular/métodos
Técnicas de Amplificação de Ácido Nucleico/métodos
Infecções por Vírus de RNA/veterinária
Vírus de RNA/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Água Doce
Infecções por Vírus de RNA/virologia
Vírus de RNA/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171021
[St] Status:MEDLINE


  5 / 3851 MEDLINE  
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[PMID]:29261765
[Au] Autor:Levy T; Rosen O; Simons O; Savaya Alkalay A; Sagi A
[Ad] Endereço:Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
[Ti] Título:The gene encoding the insulin-like androgenic gland hormone in an all-female parthenogenetic crayfish.
[So] Source:PLoS One;12(12):e0189982, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Male sexual differentiation in crustaceans is controlled by the androgenic gland (AG), a unique male endocrine organ that, in decapods, is located at the base of the 5th pereiopod. In these animals, the insulin-like androgenic gland hormone (IAG) is the major factor secreted from the AG to induce masculinization and maintain male characteristics. It has, however, recently been proposed that this hormone also plays a role in growth and ovarian development in females. In this study, we tested such a possibility by searching for the IAG gene in the marbled crayfish, a parthenogenetic animal that reproduces asexually to form an all-female genetic clone. Based on the phylogenetic relationship between the marbled crayfish and Procambarus fallax, a gonochoristic species of the same North American Cambaridae family, we searched for the IAG gene in the marbled crayfish and then fully sequenced it. The open reading frame of the gene was found to be completely identical in the two species, and their introns shared over 94% identity. It was also found that, in addition to its expression at the base of the 5th pereiopod and in the testes of male P. fallax crayfish, IAG was expressed in the muscle tissue of P. fallax males and females and even of the parthenogenetic marbled crayfish. These findings provide new insight into possible functions of IAG, in addition to its role as a masculinization-inducing factor, and also constitute the basis for a discussion of the evolutionary relationship between the above two species.
[Mh] Termos MeSH primário: Astacoidea/genética
Hormônios Gonadais/genética
Insulina/genética
Partenogênese/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Feminino
Biblioteca Gênica
Genoma
Hormônios Gonadais/química
Hormônios Gonadais/metabolismo
Insulina/química
Insulina/metabolismo
Masculino
Filogenia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gonadal Hormones); 0 (Insulin); 0 (RNA, Messenger)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189982


  6 / 3851 MEDLINE  
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[PMID]:28886062
[Au] Autor:Sacristán HJ; Rodríguez YE; De Los Angeles Pereira N; López Greco LS; Lovrich GA; Fernández Gimenez AV
[Ad] Endereço:Centro Austral de Investigaciones Científicas, CONICET, Houssay, Ushuaia, Argentina.
[Ti] Título:Energy reserves mobilization: Strategies of three decapod species.
[So] Source:PLoS One;12(9):e0184060, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In food deprivation assays, several different responses have been observed in crustaceans. However, studying energy reserves utilization among more than one species during the same starvation period has not yet been performed, particularly to discern whether the responses are due to intrinsic and/or environmental factors. We hypothesize that decapod species with similar feeding habits have the same strategies in the use of energetic reserves during starvation, even though they inhabit different environments. The aim of this study was to compare the energy reserves mobilization of three decapods species (Cherax quadricarinatus, Palaemon argentinus and Munida gregaria) with similar feeding habits, exposed to similar food deprivation conditions. The crayfish, shrimp and squat-lobster were experimentally kept at continuous feeding or continuous starvation throughout 15 days. Every 3rd day, the midgut gland index (MGI), and the glycogen, lipid and protein contents were measured in the midgut gland (MG) and pleon muscle. Palaemon argentinus mobilized more reserves during starvation, followed by C. quadricarinatus, and the last M. gregaria. The starved shrimps presented low MGI, whereas MG showed a reduction in glycogen (from day 6 to 15), lipid (from day 3 to 15), and protein levels (at day 9 and 15) while in their muscle, lipid reserves decreased at days 3 and 6. In C. quadricarinatus, the most affected parameters in the MG were MGI, glycogen (from day 6 to 15), and lipids (at day 12 and 15). In the MG of M. gregaria only the glycogen was reduced during fasting from 3 to 15 days. Even though the three studied species have similar feeding habitats, we found that their energetic profile utilization is different and it could be explained by the habitat, life span, temperature, organ/tissue, and metabolism of the species. Our results may be useful to understand the several different responses of crustaceans during starvation.
[Mh] Termos MeSH primário: Crustáceos/fisiologia
Metabolismo Energético
Privação de Alimentos
[Mh] Termos MeSH secundário: Animais
Astacoidea/fisiologia
Crustáceos/classificação
Meio Ambiente
Glicogênio/metabolismo
Metabolismo dos Lipídeos
Músculos/metabolismo
Filogenia
Inanição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9005-79-2 (Glycogen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184060


  7 / 3851 MEDLINE  
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[PMID]:28874231
[Au] Autor:Li F; Xu L; Yang F
[Ad] Endereço:1​State Key Laboratory Breeding Base of Marine Genetic Resources; Fujian Key Laboratory of Marine Genetic Resources; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of State Oceanic Administration, T
[Ti] Título:Genomic characterization of a novel iridovirus from redclaw crayfish Cherax quadricarinatus: evidence for a new genus within the family Iridoviridae.
[So] Source:J Gen Virol;98(10):2589-2595, 2017 Oct.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel iridovirus, Cherax quadricarinatus iridovirus (CQIV), was identified from diseased C. quadricarinatus in 2014. This virus is considered as a new threat to crustacean aquaculture because it is lethal to both peneaid shrimp and crayfish. Here, we determined the complete genome sequence of CQIV. The double-stranded DNA genome is 165 695 bp in length with a G+C content of 34.6 %. A total of 178 open reading frames (ORFs) have been predicted, encoding hypothetical proteins ranging from 50 to 1327 amino acids. Forty-seven of these exhibit similarities to proteins of known functions. Phylogenetic analysis based on multiple alignments of conserved proteins shows that CQIV clusters with the members of the family Iridoviridae, but is placed in a distinct clade from all the five known genera. It indicates that CQIV may represent a new genus in the family Iridoviridae, for which we propose the name Cheraxvirus based on the host organism.
[Mh] Termos MeSH primário: Astacoidea/virologia
DNA Viral/genética
Genoma Viral/genética
Iridoviridae
[Mh] Termos MeSH secundário: Animais
Composição de Bases
Sequência de Bases
Iridoviridae/classificação
Iridoviridae/genética
Iridoviridae/isolamento & purificação
Fases de Leitura Aberta/genética
Análise de Sequência de DNA
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000904


  8 / 3851 MEDLINE  
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[PMID]:28873401
[Au] Autor:Carreira BM; Segurado P; Laurila A; Rebelo R
[Ad] Endereço:cE3c Centre for Ecology Evolution and Environmental Changes, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal.
[Ti] Título:Can heat waves change the trophic role of the world's most invasive crayfish? Diet shifts in Procambarus clarkii.
[So] Source:PLoS One;12(9):e0183108, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the Mediterranean basin, the globally increasing temperatures are expected to be accompanied by longer heat waves. Commonly assumed to benefit cold-limited invasive alien species, these climatic changes may also change their feeding preferences, especially in the case of omnivorous ectotherms. We investigated heat wave effects on diet choice, growth and energy reserves in the invasive red swamp crayfish, Procambarus clarkii. In laboratory experiments, we fed juvenile and adult crayfish on animal, plant or mixed diets and exposed them to a short or a long heat wave. We then measured crayfish survival, growth, body reserves and Fulton's condition index. Diet choices of the crayfish maintained on the mixed diet were estimated using stable isotopes (13C and 15N). The results suggest a decreased efficiency of carnivorous diets at higher temperatures, as juveniles fed on the animal diet were unable to maintain high growth rates in the long heat wave; and a decreased efficiency of herbivorous diets at lower temperatures, as juveniles in the cold accumulated less body reserves when fed on the plant diet. Heat wave treatments increased the assimilation of plant material, especially in juveniles, allowing them to sustain high growth rates in the long heat wave. Contrary to our expectations, crayfish performance decreased in the long heat wave, suggesting that Mediterranean summer heat waves may have negative effects on P. clarkii and that they are unlikely to boost its populations in this region. Although uncertain, it is possible that the greater assimilation of the plant diet resulted from changes in crayfish feeding preferences, raising the hypotheses that i) heat waves may change the predominant impacts of this keystone species and ii) that by altering species' trophic niches, climate change may alter the main impacts of invasive alien species.
[Mh] Termos MeSH primário: Astacoidea/fisiologia
Dieta
Cadeia Alimentar
Temperatura Alta
Espécies Introduzidas
[Mh] Termos MeSH secundário: Animais
Isótopos de Carbono
Modelos Lineares
Isótopos de Nitrogênio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbon Isotopes); 0 (Nitrogen Isotopes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183108


  9 / 3851 MEDLINE  
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[PMID]:28750039
[Au] Autor:Martín-Torrijos L; Campos Llach M; Pou-Rovira Q; Diéguez-Uribeondo J
[Ad] Endereço:Department of Mycology, Real Jardín Botánico (RJB-CSIC), Plaza Murillo, 2, Madrid, Spain.
[Ti] Título:Resistance to the crayfish plague, Aphanomyces astaci (Oomycota) in the endangered freshwater crayfish species, Austropotamobius pallipes.
[So] Source:PLoS One;12(7):e0181226, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The pathogen Aphanomyces astaci Schikora 1906 is responsible for the decline of the native crayfish species of Europe, and their current endangered status. This pathogenic species is native to North America and only colonizes aquatic decapods. The North American crayfish species have a high resistance to this pathogen, while species from other regions are highly susceptible. However, recent field and laboratory observations indicate that there might exist some populations with resistance against this disease. The objective of this study was to test the susceptibility of 8 selected native European crayfish populations of Austropotamobius pallipes Lereboullet 1858 from the Pyrenees. We challenged them against the genome sequenced strain AP03 of A. astaci isolated from a North American red swamp crayfish, Procambarus clarkii Girard 1852, in the Garrotxa Natural Park, Girona. The results showed that there are significant differences (P<0,001) among populations, although most of them show high mortality rates after the zoospore challenge with A. astaci. However, one population from Girona exhibited a 100% survival during a four-month monitoring period under the experimental conditions tested. Histological analyses revealed a high immune reaction in tissues examined, i.e., encapsulation and melanization of hyphae, similar to that found in North American resistant crayfish species. These results represent the first observation of a native European crayfish population showing high resistance towards the most virulent genotype of this pathogen, i.e., genotype Pc. The identification of this population is of key importance for the management of these endangered species, and represents a crucial step forward towards the elucidation of the factors involved in the immune reaction against this devastating pathogen.
[Mh] Termos MeSH primário: Astacoidea/microbiologia
Resistência à Doença
Espécies em Perigo de Extinção
Água Doce
Peste/patologia
[Mh] Termos MeSH secundário: Animais
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181226


  10 / 3851 MEDLINE  
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[PMID]:28654642
[Au] Autor:Agersnap S; Larsen WB; Knudsen SW; Strand D; Thomsen PF; Hesselsøe M; Mortensen PB; Vrålstad T; Møller PR
[Ad] Endereço:Section for Evolutionary Genomics, Natural History Museum of Denmark, University of Copenhagen, Universitetsparken 15, Copenhagen Ø, Denmark.
[Ti] Título:Monitoring of noble, signal and narrow-clawed crayfish using environmental DNA from freshwater samples.
[So] Source:PLoS One;12(6):e0179261, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:For several hundred years freshwater crayfish (Crustacea-Decapoda-Astacidea) have played an important ecological, cultural and culinary role in Scandinavia. However, many native populations of noble crayfish Astacus astacus have faced major declines during the last century, largely resulting from human assisted expansion of non-indigenous signal crayfish Pacifastacus leniusculus that carry and transmit the crayfish plague pathogen. In Denmark, also the non-indigenous narrow-clawed crayfish Astacus leptodactylus has expanded due to anthropogenic activities. Knowledge about crayfish distribution and early detection of non-indigenous and invasive species are crucial elements in successful conservation of indigenous crayfish. The use of environmental DNA (eDNA) extracted from water samples is a promising new tool for early and non-invasive detection of species in aquatic environments. In the present study, we have developed and tested quantitative PCR (qPCR) assays for species-specific detection and quantification of the three above mentioned crayfish species on the basis of mitochondrial cytochrome oxidase 1 (mtDNA-CO1), including separate assays for two clades of A. leptodactylus. The limit of detection (LOD) was experimentally established as 5 copies/PCR with two different approaches, and the limit of quantification (LOQ) were determined to 5 and 10 copies/PCR, respectively, depending on chosen approach. The assays detected crayfish in natural freshwater ecosystems with known populations of all three species, and show promising potentials for future monitoring of A. astacus, P. leniusculus and A. leptodactylus. However, the assays need further validation with data 1) comparing traditional and eDNA based estimates of abundance, and 2) representing a broader geographical range for the involved crayfish species.
[Mh] Termos MeSH primário: Astacoidea/genética
Conservação dos Recursos Naturais/métodos
DNA/análise
Monitoramento Ambiental/métodos
Água Doce/química
Espécies Introduzidas
[Mh] Termos MeSH secundário: Animais
Ecossistema
Países Escandinavos e Nórdicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179261



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