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Pesquisa : B01.050.500.131.365.190.110 [Categoria DeCS]
Referências encontradas : 5249 [refinar]
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  1 / 5249 MEDLINE  
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[PMID]:28452048
[Au] Autor:Underwood CN; Davies TW; Queirós AM
[Ad] Endereço:Centre for Ecology and Conservation, Biosciences, College of Life and Environmental Sciences, University of Exeter, Penryn, Cornwall, TR10 9FE, UK.
[Ti] Título:Artificial light at night alters trophic interactions of intertidal invertebrates.
[So] Source:J Anim Ecol;86(4):781-789, 2017 07.
[Is] ISSN:1365-2656
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Despite being globally widespread in coastal regions, the impacts of light pollution on intertidal ecosystems has received little attention. Intertidal species exhibit many night-time-dependent ecological strategies, including feeding, reproduction, orientation and predator avoidance, which are likely negatively affected by shifting light regimes, as has been observed in terrestrial and aquatic taxa. Coastal lighting may shape intertidal communities through its influence on the nocturnal foraging activity of dogwhelks (Nucella lapillus), a widespread predatory mollusc that structures biodiversity in temperate rocky shores. In the laboratory, we investigated whether the basal and foraging activity of this predator was affected by exposure to night-time lighting both in the presence and absence of olfactory predator cues (Carcinus maenas, common shore crab). Assessments of dogwhelks' behavioural responses to night-time white LED lighting were performed on individuals that had been acclimated to night-time white LED lighting conditions for 16 days and individuals that had not previously been exposed to artificial light at night. Dogwhelks acclimated to night-time lighting exhibited natural refuge-seeking behaviour less often compared to control animals, but were more likely to respond to and handle prey irrespective of whether olfactory predator cues were present. These responses suggest night-time lighting likely increased the energetic demand of dogwhelks through stress, encouraging foraging whenever food was available, regardless of potential danger. Contrastingly, whelks not acclimated under night-time lighting were more likely to respond to the presence of prey under artificial light at night when olfactory predator cues were present, indicating an opportunistic shift towards the use of visual instead of olfactory cues in risk evaluation. These results demonstrate that artificial night-time lighting influences the behaviour of intertidal fauna such that the balance of interspecific interactions involved in community structuring may be affected.
[Mh] Termos MeSH primário: Braquiúros
Cadeia Alimentar
Gastrópodes
Luz
[Mh] Termos MeSH secundário: Animais
Ecossistema
Comportamento Predatório
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1111/1365-2656.12670


  2 / 5249 MEDLINE  
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[PMID]:28935584
[Au] Autor:Liu C; Jia X; Zou Z; Wang X; Wang Y; Zhang Z
[Ad] Endereço:Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen 361021, China.
[Ti] Título:VIH from the mud crab is specifically expressed in the eyestalk and potentially regulated by transactivator of Sox9/Oct4/Oct1.
[So] Source:Gen Comp Endocrinol;255:1-11, 2018 Jan 01.
[Is] ISSN:1095-6840
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vitellogenesis-inhibiting hormone (VIH) is known to regulate ovarian maturation by suppressing the synthesis of vitellogenin (Vtg) in crustaceans, which belongs to a member of crustacean hyperglycemic hormone (CHH) family synthesized and secreted from the X-organ/sinus gland complex of eyestalks. In this study, the cDNA, genomic DNA (gDNA) and the 5'-upstream regulatory (promoter region) sequences of VIH gene were obtained by conventional PCR, genome walker and tail-PCR techniques according to our transcriptomic database of Scylla paramamosain. The full-length cDNA of SpVIH is 634bp including 105bp 5'UTR, 151bp 3'UTR and 378bp ORF that encodes a peptide of 125 amino acids. The full length gDNA of SpVIH is 790bp containing two exons and one intron. The 5'-flanking promoter regions of SpVIH we isolated are 3070bp from the translation initiation (ATG) and 2398bp from the predicted transcription initiation (A), which consists of putative core promoter region and multiple potential transcription factor binding sites. SpVIH was only expressed in eyestalk. The expression level of SpVIH in eyestalk of female crab decreased gradually along with the development of ovary. As there is not cell line of crabs available, we chose the mature transfection system HEK293FT cell lines to explore the mechanism of transcription regulation of SpVIH in crabs. Sequential deletion assays using luciferase reporter gene in HEK293FT cells revealed that the possible promoter activity regions (including positive and negative transcription factors binding sites simultaneously) presented between pSpVIH-4 and pSpVIH-6. In order to further identify the crucial transcription factors binding site in this region, the site-directed mutagenesis of Sox9/Oct4/Oct1 binding site of pSpVIH-4 was created. The results demonstrated that the transcriptional activity of pSpVIH-4â–³ decreased significantly (p<0.05). Thus, it is reasonable to deduce that the Sox9/Oct4/Oct1 may be the essential positive transcription factors which regulate the expression of SpVIH.
[Mh] Termos MeSH primário: Braquiúros/metabolismo
Proteínas de Transporte/metabolismo
Olho/metabolismo
Hormônios de Invertebrado/metabolismo
Fator 1 de Transcrição de Octâmero/metabolismo
Fator 3 de Transcrição de Octâmero/metabolismo
Fatores de Transcrição SOX9/metabolismo
Transativadores/metabolismo
[Mh] Termos MeSH secundário: Região 5'-Flanqueadora/genética
Sequência de Aminoácidos
Animais
Sequência de Bases
Proteínas de Transporte/química
Proteínas de Transporte/genética
DNA Complementar/genética
Feminino
Regulação da Expressão Gênica no Desenvolvimento
Células HEK293
Seres Humanos
Hormônios de Invertebrado/química
Hormônios de Invertebrado/genética
Mutação/genética
Ovário/embriologia
Ovário/metabolismo
Filogenia
Regiões Promotoras Genéticas/genética
Análise de Sequência de DNA
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (DNA, Complementary); 0 (Invertebrate Hormones); 0 (Octamer Transcription Factor-1); 0 (Octamer Transcription Factor-3); 0 (SOX9 Transcription Factor); 0 (Trans-Activators); 138360-48-2 (vitellogenesis inhibiting hormone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE


  3 / 5249 MEDLINE  
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[PMID]:29307851
[Au] Autor:Bai J; Xu S; Nie Z; Wang Y; Zhu C; Wang Y; Min W; Cai Y; Zou J; Zhou X
[Ad] Endereço:Research lab of Freshwater Crustacean Decapoda &Paragonimus, School of Basic Medical Sciences, Nanchang University, 461 Bayi Avenue, Nanchang City, Jiangxi Province 330006, People's Republic of China.
[Ti] Título:The complete mitochondrial genome of Huananpotamon lichuanense (Decapoda: Brachyura) with phylogenetic implications for freshwater crabs.
[So] Source:Gene;646:217-226, 2018 Mar 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In the present study, we determined the complete mitochondrial genome of Huananpotamon lichuanense (Decapoda: Brachyura) for the first time. The genome is 15,380bp in length and typically consists of 37 genes. When the gene order was compared to the ancestral crustacean type, two tRNA genes (tRNA and tRNA ) were rearranged in H. lichuanense, and the translocation of tRNA appeared only in Potamoidea crabs, such as Geothelphusa dehaani and Sinopotamon xiushuiense, supporting the monophyly of the Potamoidea superfamily. Thirteen protein-coding genes and 2 rRNA genes were divided into five complexes to perform the phylogenetic analysis, and the results showed that the trees constructed by complex I (ND1-ND6 and ND4L), complex IV (COX1-COX3) and rRNA genes better accord with the morphological classification system, suggesting that molecular markers of higher-level phylogeny can be developed in these three complexes in the future. The estimated divergence time for freshwater crabs is approximately 133.58Ma, and G. dehaani from Japan diverged from the freshwater crabs of mainland China approximately 60.66Ma. A selective pressure analysis based on current data revealed obviously increasing dN/dS ratios (except for ATP6 and ND4L) of freshwater crabs, and the accumulation of nonsynonymous mutations suggests that terrestrial habitats provide a relatively relaxed selective pressure environment for this group.
[Mh] Termos MeSH primário: Braquiúros/genética
Genoma Mitocondrial
Mitocôndrias/genética
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Animais
Evolução Molecular
Ordem dos Genes
Tamanho do Genoma
Masculino
Filogenia
RNA de Transferência/genética
Seleção Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


  4 / 5249 MEDLINE  
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[PMID]:29288727
[Au] Autor:Li Q; Yang H; He L; Wang Q
[Ad] Endereço:College of Ecological Engineering, Guizhou University of Engineering Science, College Road, Bijie City, Guizhou Province 551700, China.
[Ti] Título:Characterization of the Es-DDX52 involved in the spermatogonial mitosis and spermatid differentiation in Chinese mitten crab (Eriocheir sinensis).
[So] Source:Gene;646:106-119, 2018 Mar 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Spermatogenesis involves a series of process including exiting from the mitotic cell cycle, entry into meiosis, completion of complex differentiation programs, and producing spermatozoa. Expression of various genes in an ordered manner, and interactions between various genes and their protein products, primarily controlled at the post-transcriptional level with DEAD-box RNA helicases playing a crucial role in germ cell development, are required for production of fertile sperm. Many members of this family have been deeply studied in spermatogenesis, such as DDX3X, DDX25 and DDX4, but few data are available on DDX52. In this study, we analyzed the expression patterns of Es-DDX52, Es-DDX6, Es-Vasa and Es-XRN1 both at mRNA and protein levels in different tissues and during gonadal development. It showed that Es-vasa, Es-DDX6 and Es-Xrn1, components of cytoplasmic foci P-bodies, have the similar transcriptional expression pattern, while Es-DDX52 has the reverse tendency. Furthermore, Es-DDX6 and Es-XRN1 proteins have the same localization in testicular tissues. Es-DDX52 mainly distributed in the cytoplasm of spermatogonia, only localized in the nucleus of early and middle spermatid and shifted to pre-acrosome vesicle (later developed into apical cap and acrosome tube) at both mRNA and protein levels. These results indicated that Es-DDX52 may participate in regulation of P-bodies and microtubules by Es-XRN1, and involved in the mitosis of spermatonia and spermatid differentiation.
[Mh] Termos MeSH primário: Braquiúros/metabolismo
RNA Helicases DEAD-box/genética
RNA Helicases DEAD-box/metabolismo
Espermátides/citologia
Espermatogônias/fisiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Artrópodes/metabolismo
Diferenciação Celular
Clonagem Molecular
RNA Helicases DEAD-box/química
Regulação da Expressão Gênica
Masculino
Mitose
Modelos Moleculares
Filogenia
Espermátides/metabolismo
Espermatogênese
Espermatogônias/metabolismo
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); EC 3.6.4.13 (DEAD-box RNA Helicases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


  5 / 5249 MEDLINE  
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[PMID]:29250542
[Au] Autor:Fu C; Zeng Q; Li F; Wang H; Sun J; Wang H
[Ad] Endereço:College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China.
[Ti] Título:Comparative Transcriptome Analysis Reveals Related Regulatory Mechanisms of Androgenic Gland in .
[So] Source:Biomed Res Int;2017:4956216, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chinese mitten crab is one of the most commercially important aquaculture species in China. The androgenic gland (AG) of crustaceans plays pivotal roles in the regulation of male differentiation and in maintaining the male sexual characteristics. In order to reveal related mechanisms in AG, we compared transcriptomes of AG between proliferation and secretion phase. A total of 72,000 unigenes and 4,027 differentially expressed genes were obtained. Gene ontology enrichment analysis indicated that biological processes and metabolic pathways related to protein synthesis and secretion such as transcription, translation, and signal transduction were significantly enriched. Critical genes such as , and were identified and potentially involved in maintaining the testis development and spermatogenesis. Ribosomes pathway revealed the cause of insulin-like androgenic gland hormone secretion increase. Three insulin-like receptors were thought to be associated with growth and spermatogenesis. In the neuroactive ligand-receptor interaction pathway, the expression of octopamine receptor, 5-HT receptor 1, and melatonin receptor was significantly changed, which revealed the key regulation mechanism of aggressive and mating behavior of males. Comparative transcriptome analysis provided new insights into the genome-wide molecular mechanisms of AG development and the regulatory mechanisms of male development.
[Mh] Termos MeSH primário: Androgênios/metabolismo
Braquiúros/fisiologia
Regulação da Expressão Gênica/fisiologia
Transcriptoma/fisiologia
[Mh] Termos MeSH secundário: Animais
Braquiúros/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica/genética
Ontologia Genética
Masculino
Comportamento Sexual Animal/fisiologia
Transdução de Sinais
Transcriptoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Androgens)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1155/2017/4956216


  6 / 5249 MEDLINE  
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[PMID]:29180238
[Au] Autor:Asaro A; Paggi RA; Del Valle JC; López Mañanes AA
[Ad] Endereço:Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, Argentina.
[Ti] Título:Glucose homeostasis in the euryhaline crab Cytograpsus angulatus: Effects of the salinity in the amylase, maltase and sucrase activities in the hepatopancreas and in the carbohydrate reserves in different tissues.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;216:39-47, 2018 Feb.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We studied the existence, biochemical characteristics and response to different environmental salinities of amylase, maltase and sucrase activity in the intertidal euryhaline crab Cyrtograpsus angulatus (Dana, 1852) along with the response to distinct salinities of glycogen and free glucose content in storage organs. Amylase, maltase and sucrase activities were kept over a broad range of pH and temperature and exhibited Michaelis-Menten kinetics. Zymography showed the existence of two amylase forms in crabs exposed to 35 (osmoconformation) and low (6-10psu; hyper-regulation) or high (40psu) (hypo-regulation) salinities. Carbohydrases activity in the hepatopancreas and glycemia were not affected in crab exposed to different environmental salinities. In 6 and 40psu, the glycogen content in anterior gills was lower than in 35psu. In 6, 10 and 40psu, glycogen concentration in hepatopancreas, muscle and posterior gills were similar to that in 35psu. Free glucose concentration in chela muscle was higher in 6 and 40psu than in 35psu. The existence and biochemical characteristics of carbohydrases activity and the adjustments in concentration of glycogen in anterior gills and free glucose in chela muscle suggests the ability to perform complete hydrolysis of glycogenic substrates and to keep glucose homeostasis in relation to acclimation to different salinity conditions.
[Mh] Termos MeSH primário: Amilases/metabolismo
Proteínas de Artrópodes/metabolismo
Braquiúros/metabolismo
Glucose/metabolismo
Hepatopâncreas/metabolismo
Salinidade
Sacarase/metabolismo
alfa-Glucosidases/metabolismo
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); EC 3.2.1.- (Amylases); EC 3.2.1.20 (alpha-Glucosidases); EC 3.2.1.48 (Sucrase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


  7 / 5249 MEDLINE  
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[PMID]:29206569
[Au] Autor:McGaw IJ; Steell SC; Leeuwen TEV; Eliason EJ; Cooke SJ
[Ti] Título:Application of Miniature Heart Rate Data Loggers for Use in Large Free-Moving Decapod Crustaceans: Method Development and Validation.
[So] Source:Physiol Biochem Zool;91(1):731-739, 2018 Jan/Feb.
[Is] ISSN:1537-5293
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cardiovascular responses of decapod crustaceans to environmental challenges have received extensive attention. However, nearly all of these studies have been restricted to lab-based experiments; here we describe a methodology that will enable measurement of heart rate (HR) in free-moving decapods in the field. Data storage tag heart rate and temperature loggers (DST micro-HRT; Star-Oddi) were used to record electrocardiograms (ECG) and HR in large decapod crustaceans. These loggers were originally designed for use in vertebrates and must be surgically implanted in the body cavity near the heart in order to function. We adapted these loggers for external use in large decapod crustaceans. The method involved abrading the carapace directly above the heart and placing the electrodes of the logger directly on top of the dermal tissue. The logger was then secured in place with periphery wax. This method negated some of the more intricate operations used for vertebrates. The rapid setup time of approximately 5 min suggested that animals could be easily instrumented in the field and without the use of anesthetic. The logger was calibrated by simultaneously measuring the HR changes of a West Indian spider crab Mithrax spinosissimus with a pulsed-Doppler flowmeter. The data gathered with the two methods showed a tight correlation during an increase in temperature. The loggers were also successfully implanted in a variety of other large species of aquatic and terrestrial decapods. The data obtained showed that the method works in a broad range of species, under different experimental conditions. In each case, the loggers comprised less than 1% of the body mass and would be suitable for use in animals >300 g. All animals survived the attachment procedures and were feeding and active after removal of the loggers. Nearly all previous cardiac measurements on decapods have been carried out in controlled laboratory settings. The use of these loggers will make significant advances in measuring HR in unrestrained, undisturbed animals in their natural environment during extended periods of time and has the potential to lead to novel findings.
[Mh] Termos MeSH primário: Braquiúros/fisiologia
Frequência Cardíaca/fisiologia
Monitorização Fisiológica/instrumentação
[Mh] Termos MeSH secundário: Animais
Atividade Motora
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1086/695839


  8 / 5249 MEDLINE  
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[PMID]:29031772
[Au] Autor:Meng X; Zhang X; Li J; Liu P
[Ad] Endereço:Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, People's Republic of China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National La
[Ti] Título:Identification and comparative profiling of ovarian and testicular microRNAs in the swimming crab Portunus trituberculatus.
[So] Source:Gene;640:6-13, 2018 Jan 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:MicroRNAs (miRNAs) are a class of endogenous non-coding small RNA, known as key post-transcriptional regulators for development and function of reproductive organ in animals. However, limited information is currently available on the regulatory roles of miRNAs in crustacean reproduction. In the present study, we performed small RNA deep sequencing in ovary and testis tissues of the swimming crab Portunus trituberculatus, which is a commercially important species in East Asian countries, to identify and characterize the miRNAs in gonads. We obtained 187 and 225 miRNAs from ovary and testis, respectively, including 188 known and 65 novel miRNAs. Expression profiling of the identified miRNAs indicated that ovary and testis shared the same six most abundant miRNAs (let-7, let-7c, let-7f, mir-2, mir-184 and mir-276), which accounted for a large proportion of total reads in gonads (35.78% for ovary and 37.01% for testis) and indicated that they may play essential roles in gonadal development and function. Statistically, 75 miRNAs showed differential expression level between ovary and testis tissues, and the putative target genes of these miRNAs were significantly enriched in 14 KEGG pathways, including several pathways important in reproduction, such as GnRH signaling pathway, Wnt signaling pathway, ovarian steroidogenesis and TGF-beta signaling pathway. Further analysis found that the differentially expressed miRNAs exhibited stage-specific expression during ovarian and testicular development, suggesting their potential roles in reproductive development. Our study provided the first insight into regulatory roles of gonadal miRNAs and would serve as a valuable resource for investigating miRNA-mediated mechanisms of reproduction regulation in P. trituberculatus and other crustaceans.
[Mh] Termos MeSH primário: Braquiúros/genética
Perfilação da Expressão Gênica
Sequenciamento de Nucleotídeos em Larga Escala/métodos
MicroRNAs/genética
Ovário/metabolismo
Testículo/metabolismo
[Mh] Termos MeSH secundário: Animais
Braquiúros/crescimento & desenvolvimento
Biologia Computacional
Feminino
Regulação da Expressão Gênica
Masculino
Ovário/citologia
Natação
Testículo/citologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE


  9 / 5249 MEDLINE  
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[PMID]:28464390
[Au] Autor:Farias NE; Spivak ED; Luppi TA
[Ad] Endereço:Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de investigaciones Científicas y Tecnológicas (CONICET), Universidad Nacional de Mar del Plata, Departamento de Biología, CC 1260, Mar del Plata, 7600, Argentina.
[Ti] Título:Functional morphology of the female reproductive system of a crab with highly extensible seminal receptacles and extreme sperm storage capacity.
[So] Source:J Morphol;278(7):919-935, 2017 Jul.
[Is] ISSN:1097-4687
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We studied the functional morphology of the female reproductive system of the purple stone crab Danielethus crenulatus. The most remarkable feature is the relative storage capacity and extensibility of the seminal receptacles. These receptacles are a pair of simple sacs that lack internal structures dividing the internal lumen. Differences in seminal receptacle size and contents are accompanied by conspicuous changes in receptacle lining at a tissue level. Full seminal receptacles contain discrete sperm masses formed by hardened fluid and densely packed spermatophores. Different sperm masses are likely from different mates and their stratified disposition within the seminal receptacles is compatible with rival sperm displacement and last sperm precedence. Additionally, the anatomical structure of the vulva and vagina suggest active female control over copula. We discuss our results in the general context of sperm storage in brachyurans and the implications for the mating system of this species.
[Mh] Termos MeSH primário: Braquiúros/anatomia & histologia
Braquiúros/fisiologia
Genitália Feminina/anatomia & histologia
Genitália Feminina/fisiologia
Glândulas Seminais/fisiologia
Espermatozoides/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Genitália Feminina/citologia
Masculino
Reprodução
Comportamento Sexual Animal
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1002/jmor.20685


  10 / 5249 MEDLINE  
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[PMID]:28951248
[Au] Autor:Li R; Zhu LN; Ren LQ; Weng JY; Sun JS
[Ad] Endereço:Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Science, Tianjin Normal University, Tianjin, People's Republic of China.
[Ti] Título:Molecular cloning and characterization of glycogen synthase in Eriocheir sinensis.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;214:47-56, 2017 Dec.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Glycogen plays an important role in glucose and energy homeostasis at cellular and organismal levels. In glycogen synthesis, glycogen synthase (GS) is a rate-limiting enzyme catalysing the addition of α-1,4-linked glucose units from (UDP) -glucose to a nascent glycogen chain using glycogenin (GN) as a primer. While studies on mammalian liver GS (GYS2) are numerous, enzymes from crustaceans, which also use glycogen and glucose as their main energy source, have received less attention. In the present study, we amplified full-length GS cDNA from Eriocheir sinensis. Tissue expression profiling revealed the highest expression of GS in the hepatopancreas. During moulting, GS expression and activity declined, and glycogen levels in the hepatopancreas were reduced. Recombinant GS was expressed in Escherichia coli Rosetta (DE3), and induction at 37°C or 16°C yielded EsGS in insoluble inclusion bodies (EsGS-I) or in soluble form (EsGS-S), respectively. Enzyme activity was measured in a cell-free system containing glucose-6-phosphate (G6P), and both forms possessed glycosyltransferase activity, but refolded EsGS-I was more active. Enzyme activity of both GS and EsGS-I in the hepatopancreas was optimum at 25°C, which is coincident with the optimum growth temperature of Chinese mitten crab, and higher (37°C) or lower (16°C) temperatures resulted in lower enzyme activity. Taken together, the results suggest that GS may be important for maintaining normal physiological functions such as growth and reproduction.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/genética
Braquiúros/enzimologia
Glicogênio Sintase/genética
Glicogênio/biossíntese
Hepatopâncreas/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas de Artrópodes/metabolismo
Sítios de Ligação
Braquiúros/genética
Braquiúros/crescimento & desenvolvimento
Clonagem Molecular
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Glucose-6-Fosfato/metabolismo
Glucosiltransferases/metabolismo
Glicogênio Sintase/metabolismo
Glicoproteínas/metabolismo
Hepatopâncreas/crescimento & desenvolvimento
Corpos de Inclusão/química
Cinética
Muda/genética
Especificidade de Órgãos
Filogenia
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (Glycoproteins); 0 (Recombinant Proteins); 0 (glycogenin); 56-73-5 (Glucose-6-Phosphate); 9005-79-2 (Glycogen); EC 2.4.1.- (Glucosyltransferases); EC 2.4.1.11 (Glycogen Synthase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE



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