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[PMID]:29182506
[Au] Autor:Battelle BA
[Ti] Título:Opsins and Their Expression Patterns in the Xiphosuran Limulus polyphemus.
[So] Source:Biol Bull;233(1):3-20, 2017 Aug.
[Is] ISSN:1939-8697
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The American horseshoe crab Limulus polyphemus (Linnaeus, 1758) is one of four extant species of xiphosuran chelicerates, the sister group to arachnids. Because of their position in the arthropod family tree and because they exhibit many plesiomorphic characteristics, Xiphosura are considered a proxy for the euchelicerate ancestor and therefore important for understanding the evolution and diversification of chelicerates and arthropods. Limulus polyphemus is the most extensively studied xiphosuran, and its visual system has long been a focus of studies critical for our understanding of basic mechanisms of vision and the evolution of visual systems in arthropods. Building upon a wealth of information about the anatomy and physiology of its visual system, advances in genetic approaches have greatly expanded possibilities for understanding its biochemistry. This review focuses on studies of opsin expression in L. polyphemus, which have been significantly advanced by the availability of transcriptomes and a recent high-quality assembly of its genome. These studies show that the repertoire of expressed opsins in L. polyphemus is far larger than anticipated, that the regulation of their expression in rhabdoms is far more complex than anticipated, and that photosensitivity may be distributed widely throughout the L. polyphemus central nervous system. The visual system of L. polyphemus is now arguably the best understood among chelicerates, and as such, it is a critical resource for furthering our understanding of the evolution and diversification of visual systems in arthropods.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica
Caranguejos Ferradura/genética
Caranguejos Ferradura/metabolismo
Opsinas/genética
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Genoma
Caranguejos Ferradura/classificação
Fenômenos Fisiológicos Oculares/genética
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Opsins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1086/693730


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[PMID]:28693666
[Au] Autor:Abate W; Sattar AA; Liu J; Conway ME; Jackson SK
[Ad] Endereço:1​Centre for Biomedical Research, School of Biomedical and Healthcare Sciences, Peninsula Schools of Medicine and Dentistry, Plymouth University, Plymouth, UK.
[Ti] Título:Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate-based assays and a human monocyte activity assay.
[So] Source:J Med Microbiol;66(7):888-897, 2017 Jul.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The Limulus amebocytelysate (LAL) assay is widely used for the screening of lipopolysaccharide (LPS) in parenteral pharmaceuticals. However, correlation of LPS in Gram-negative bacterial infections by LAL assay has been problematic, partly due to the variable reactivity of different LPS structures. Recombinant factor C (rFC) has allowed the development of a new simple, specific and sensitive LPS detection system (PyroGene). In this work, the potential of the new assay for detecting various LPS structures has been investigated and compared with two LAL-based assays and a human monocyte activity assay. METHODOLOGY: The activity of the various LPS structures has been investigated by PyroGene and two LAL-based assays and a human monocyte activity assay. RESULTS: The rFC assay detected most LPS structures in picogram quantities and the potency of E. coli, B. cepacia, Salmonella smooth and Salmonella R345 LPS was no different when measured with PyroGene or LAL assays. However, the reactivity of K. pneumoniae, S. marcescens, B. pertussis and P. aeruginosa LPS differed significantly between these assays. Importantly, pairwise correlation analysis revealed that only the PyroGene assay produced a significant positive correlation with the release of IL-6 from a monocytic cell line. CONCLUSION: We conclude that the rFC-based assay is a good replacement for conventional LAL assays and as it correlates significantly with IL-6 produced by a human monocyte cell line it could potentially be more useful for detecting LPS in a clinical setting.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/metabolismo
Endotoxinas/análise
Precursores Enzimáticos/metabolismo
Lipopolissacarídeos/análise
Proteínas Recombinantes/metabolismo
Serina Endopeptidases/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas de Artrópodes/genética
Bactérias/química
Técnicas de Química Analítica
Precursores Enzimáticos/genética
Caranguejos Ferradura
Seres Humanos
Proteínas Recombinantes/genética
Serina Endopeptidases/genética
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (Endotoxins); 0 (Enzyme Precursors); 0 (Lipopolysaccharides); 0 (Recombinant Proteins); EC 3.4.21.- (Serine Endopeptidases); EC 3.4.21.84 (Limulus clotting factor C)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000510


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[PMID]:28322142
[Au] Autor:Ishimoto Y; Ishibashi KI; Yamanaka D; Adachi Y; Ito H; Igami K; Miyazaki T; Ohno N
[Ad] Endereço:Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.
[Ti] Título:Enhanced Release of Immunostimulating ß-1,3- Glucan by Autodigestion of the Lingzhi Medicinal Mushroom, Ganoderma lingzhi (Agaricomycetes).
[So] Source:Int J Med Mushrooms;19(1):1-16, 2017.
[Is] ISSN:1940-4344
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ganoderma lingzhi is a widely used medicinal mushroom that has antioxidative effects, ameliorates insulin resistance, and improves quality of life in patients with metabolic syndrome. Potentiation of immunity is also a major function of G. lingzhi, and this has been applied in patients with cancer. Supplementing G. lingzhi into foods reduced the metastasis of cancer cells. ß-l,3-glucan is an important bioactive component of G. lingzhi. In this study we enhanced the solubilization ofimmunostimulating ß-l,3-glucan by autodigestion of G. lingzhi. Fruiting bodies of G. lingzhi were disrupted and suspended in distilled water, then autodigested at 37°C for 24 hours. The resulting suspension was dried by spray drying. To assess the solubilization of ß-l,3-glucan by autodigestion, cold and hot water extracts and sodium hydroxide extracts of G. lingzhi were prepared with and without autodigestion. Sodium hydroxide extracts were neutralized and dialyzed against distilled water. The resulting soluble and precipitated fractions were collected. Chemical, biochemical, and immunochemical characteristics of the extracts were compared. The yields of cold water extracts of autodigested and native G. lingzhi were significantly lower than the other extracts. Glucose was the major sugar component of the hot water extract, cold alkali extract (CAS), and the cold hydroxide extract insoluble in neutral aqueous condition (CASP) of the autodigested and native G. lingzhi. Nuclear magnetic resonance analysis revealed branched ß-glucans in the hot water extract and CAS of the autodigested and native G. lingzhi. By contrast, the CASP of the autodigested and native G. lingzhi comprised mainly mixtures of linear α-l,3-glucans and linear ß-l,3-glucans. Immunostimulation by ß-l,3-glucan was examined by limulus factor G activation, dectin-1 binding, and anti-ß-glucan antibody binding. Comparing relative activity, immunostimulating ß-l,3-glucan was detected in the hot water extract, rather than the CAS, of autodigested and native G. lingzhi. Immunostimulating of ß-glucan was also detected in the cold water extract of the autodigested G. lingzhi. These findings demonstrate that autodigestion is a useful processing protocol for enhancing the usefulness of G. lingzhi as a functional food.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/análise
Misturas Complexas/química
Carpóforos/química
Ganoderma/química
Fatores Imunológicos/análise
beta-Glucanas/análise
[Mh] Termos MeSH secundário: Animais
Misturas Complexas/isolamento & purificação
Caranguejos Ferradura
Fatores Imunológicos/metabolismo
Espectroscopia de Ressonância Magnética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Complex Mixtures); 0 (Immunologic Factors); 0 (beta-Glucans); 9051-97-2 (beta-1,3-glucan)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1615/IntJMedMushrooms.v19.i1.10


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[PMID]:28187312
[Au] Autor:Fujita Y; Yagi K
[Ad] Endereço:Biological Research Department, Sawai Pharmaceutical Co., Ltd., Osaka, Japan. Electronic address: y.fujita@sawai.co.jp.
[Ti] Título:Glucose phosphorylated on carbon 6 suppresses lipopolysaccharide binding to lipopolysaccharide-binding protein and inhibits its bioactivities.
[So] Source:Int Immunopharmacol;45:43-52, 2017 Apr.
[Is] ISSN:1878-1705
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Lipid A comprises the active region of lipopolysaccharide (LPS), and its phosphate group is required for LPS activities. Additionally, it is essential for effects of inhibitors of LPS-induced coagulation activity in limulus amebocyte lysate (LAL) tests. Lipid A has phosphorylated glucosamine residues, which are structurally similar to glucose 1-phosphate (G1P) and glucose 6-phosphate (G6P). This study focused on the antagonistic effects of glucose phosphates on the action of protein or non-protein inhibitors against LAL coagulation, LPS-LPS-binding protein (LBP) interaction, and LPS bioactivities. These effects of glucose phosphates were evaluated and compared with those of other charged sugars such as fructose 6-phosphate and glucuronic acid by LAL tests, ELISA-based LPS-LBP binding assay, cell-based assay, and using a mouse endotoxin shock model. G6P neutralized the interfering actions of drug substances and plasma proteins on LPS coagulation activity in LAL tests. Compared to other sugars, G6P more strongly inhibited LPS binding to LBP, leading to significant inhibition of LPS-induced cellular responses in human umbilical vein endothelial cells and in the THP-1 human leukemic line. Consistent herewith, G6P inhibited inflammatory cytokine release and decreased serum alanine aminotransferase and hepatic caspase-3/7 activities and mortality in LPS-stimulated d-galactosamine-sensitized mice. These data indicated that the structural properties of G6P, such as its glucose moiety and phosphorylation on carbon 6, are important for suppressing the interaction of proteins with LPS. Therefore, G6P is useful to improve sensitivity and accuracy of plasma and drug LPS assays, and such structural property is more suitable to antagonize LPS activities.
[Mh] Termos MeSH primário: Proteínas da Fase Aguda/metabolismo
Proteínas de Transporte/metabolismo
Glucose/metabolismo
Lipídeo A/metabolismo
Lipopolissacarídeos/metabolismo
Glicoproteínas de Membrana/metabolismo
Nefelometria e Turbidimetria/métodos
[Mh] Termos MeSH secundário: Animais
Carbono
Caspase 3/metabolismo
Glucose/química
Caranguejos Ferradura
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Fosfatos/química
Fosforilação
Ligação Proteica
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acute-Phase Proteins); 0 (Carrier Proteins); 0 (Lipid A); 0 (Lipopolysaccharides); 0 (Membrane Glycoproteins); 0 (Phosphates); 0 (Tumor Necrosis Factor-alpha); 0 (lipopolysaccharide-binding protein); 7440-44-0 (Carbon); EC 3.4.22.- (Caspase 3); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE


  5 / 1424 MEDLINE  
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[PMID]:27913792
[Au] Autor:Mizumura H; Ogura N; Aketagawa J; Aizawa M; Kobayashi Y; Kawabata SI; Oda T
[Ad] Endereço:1 LAL Research and Development Department, Seikagaku Corporation, Tokyo, Japan.
[Ti] Título:Genetic engineering approach to develop next-generation reagents for endotoxin quantification.
[So] Source:Innate Immun;23(2):136-146, 2017 Feb.
[Is] ISSN:1753-4267
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The bacterial endotoxin test, which uses amebocyte lysate reagents of horseshoe crab origin, is a sensitive, reproducible and simple assay to measure endotoxin concentration. To develop sustainable raw materials for lysate reagents that do not require horseshoe crabs, three recombinant protease zymogens (factor C, derived from mammalian cells; factor B; and the proclotting enzyme derived from insect cells) were prepared using a genetic engineering technique. Recombinant cascade reagents (RCRs) were then prepared to reconstruct the reaction cascade in the amebocyte lysate reagent. The protease activity of the RCR containing recombinant factor C was much greater than that of recombinant factor C alone, indicating the efficiency of signal amplification in the cascade. Compared with the RCR containing the insect cell-derived factor C, those containing mammalian cell-derived factor C, which features different glycosylation patterns, were less susceptible to interference by the injectable drug components. The standard curve of the RCR containing mammalian cell-derived recombinant factor C had a steeper slope than the curves for those containing natural lysate reagents, suggesting a greater sensitivity to endotoxin. The present study supports the future production of recombinant reagents that do not require the use of natural resources.
[Mh] Termos MeSH primário: Fator B do Complemento/metabolismo
Endopeptidases/metabolismo
Endotoxinas/análise
Precursores Enzimáticos/metabolismo
Proteínas de Insetos/metabolismo
Teste do Limulus/métodos
Serina Endopeptidases/metabolismo
[Mh] Termos MeSH secundário: Animais
Extratos Celulares
Fator B do Complemento/genética
Endopeptidases/genética
Precursores Enzimáticos/genética
Engenharia Genética
Caranguejos Ferradura
Indicadores e Reagentes
Proteínas de Insetos/genética
Proteínas Recombinantes/genética
Padrões de Referência
Sensibilidade e Especificidade
Serina Endopeptidases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Extracts); 0 (Endotoxins); 0 (Enzyme Precursors); 0 (Indicators and Reagents); 0 (Insect Proteins); 0 (Recombinant Proteins); EC 3.4.- (Endopeptidases); EC 3.4.21.- (Serine Endopeptidases); EC 3.4.21.47 (Complement Factor B); EC 3.4.99.- (pro-clotting enzyme)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161204
[St] Status:MEDLINE
[do] DOI:10.1177/1753425916681074


  6 / 1424 MEDLINE  
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[PMID]:27866343
[Au] Autor:Bakker AK; Dutton J; Sclafani M; Santangelo N
[Ad] Endereço:Department of Biology, Hofstra University, Hempstead, NY, 11549, USA. Aaron.K.Bakker@outlook.com.
[Ti] Título:Maternal transfer of trace elements in the Atlantic horseshoe crab (Limulus polyphemus).
[So] Source:Ecotoxicology;26(1):46-57, 2017 Jan.
[Is] ISSN:1573-3017
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The maternal transfer of trace elements is a process by which offspring may accumulate trace elements from their maternal parent. Although maternal transfer has been assessed in many vertebrates, there is little understanding of this process in invertebrate species. This study investigated the maternal transfer of 13 trace elements (Ag, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn) in Atlantic horseshoe crab (Limulus polyphemus) eggs and compared concentrations to those in adult leg and gill tissue. For the majority of individuals, all trace elements were transferred, with the exception of Cr, from the female to the eggs. The greatest concentrations on average transferred to egg tissue were Zn (140 µg/g), Cu (47.8 µg/g), and Fe (38.6 µg/g) for essential elements and As (10.9 µg/g) and Ag (1.23 µg/g) for nonessential elements. For elements that were maternally transferred, correlation analyses were run to assess if the concentration in the eggs were similar to that of adult tissue that is completely internalized (leg) or a boundary to the external environment (gill). Positive correlations between egg and leg tissue were found for As, Hg, Se, Mn, Pb, and Ni. Mercury, Mn, Ni, and Se were the only elements correlated between egg and gill tissue. Although, many trace elements were in low concentration in the eggs, we speculate that the higher transfer of essential elements is related to their potential benefit during early development versus nonessential trace elements, which are known to be toxic. We conclude that maternal transfer as a source of trace elements to horseshoe crabs should not be overlooked and warrants further investigation.
[Mh] Termos MeSH primário: Monitoramento Ambiental
Caranguejos Ferradura/metabolismo
Exposição Materna/estatística & dados numéricos
Oligoelementos/análise
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Animais
Feminino
Mercúrio/metabolismo
Distribuição Tecidual
Oligoelementos/metabolismo
Poluentes Químicos da Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Trace Elements); 0 (Water Pollutants, Chemical); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161121
[St] Status:MEDLINE
[do] DOI:10.1007/s10646-016-1739-2


  7 / 1424 MEDLINE  
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[PMID]:27820903
[Au] Autor:Funch P; Wang T; Pertoldi C; Middelbo AB
[Ti] Título:Low Oxygen Levels Slow Embryonic Development of Limulus polyphemus.
[So] Source:Biol Bull;231(2):113-119, 2016 Oct.
[Is] ISSN:1939-8697
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The American horseshoe crab Limulus polyphemus typically spawns in the upper intertidal zone, where the developing embryos are exposed to large variations in abiotic factors such as temperature, humidity, salinity, and oxygen, which affect the rate of development. It has been shown that embryonic development is slowed at both high and low salinities and temperatures, and that late embryos close to hatching tolerate periodic hypoxia. In this study we investigated the influence of hypoxia on both early and late embryonic development in L. polyphemus under controlled laboratory conditions. Embryos were exposed to four different oxygen levels and their developmental stage was scored every second day. Embryos developed more slowly at both 5% O and 10% O than at the 21% O treatment; late development was arrested when oxygen was reduced to 2%. Our study confims that L. polyphemus not only tolerates pronounced hypoxia in later embryonic developmental stages, but also in earlier, previously unexplored, developmental stages.
[Mh] Termos MeSH primário: Desenvolvimento Embrionário/efeitos dos fármacos
Caranguejos Ferradura/efeitos dos fármacos
Caranguejos Ferradura/embriologia
Oxigênio/farmacologia
[Mh] Termos MeSH secundário: Animais
Embrião não Mamífero
Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
S88TT14065 (Oxygen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161108
[St] Status:MEDLINE


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[PMID]:27633674
[Au] Autor:Xie H; Wei J; Qin Q
[Ad] Endereço:Department of Food and Bioengineering, Bengbu College, Bengbu, 233030, PR China.
[Ti] Título:Antiviral function of Tachyplesin I against iridovirus and nodavirus.
[So] Source:Fish Shellfish Immunol;58:96-102, 2016 Nov.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Antimicrobial peptides (AMPs) are ubiquitously found in living organisms and are an important component in innate immune response. Tachyplesin I is a potent antimicrobial peptide isolated from the hemocytes of the horseshoe crab, Tachypleus tridentatus. Previous studies have shown that the 17-residue peptide exhibits a wide spectrum of antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, protozoa, and viruses. However, the efficiencies and defense mechanisms of the Tachyplesin I against fish viruses are still unknown. In this study, Tachyplesin I showed a key role in inhibiting the infection and replication of two kinds of newly emerging marine fish viruses, an enveloped DNA virus of Singapore grouper iridovirus (SGIV), and a non-enveloped RNA virus of viral nervous necrosis virus (RGNNV). Synthetic peptides of Tachyplesin I incubated with virus or cells before infection reduced the viral infectivity. Synthetic peptides of Tachyplesin I drastically decreased SGIV and RGNNV titers and viral gene expression. Grouper spleen (GS) and brain (GB) cells over-expressing Tachyplesin I (GS/pcDNA3.1-flag-Tac I and GB/pcDNA3.1-flag-Tac I) support the inhibition of viral infection. Tachyplesin I activated type I IFN and Interferon-sensitive response element (ISRE) in vitro. The promoter activity of IFN-ß and ISRE were significantly up-regulated in cells transfected with pcDNA3.1-flag-Tac I after infection with SGIV and VNNV. These results suggest that Tachyplesin I is importantly involved in host immune responses to invasion of viral pathogens.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/metabolismo
Proteínas de Artrópodes/metabolismo
Proteínas de Ligação a DNA/metabolismo
Caranguejos Ferradura/imunologia
Caranguejos Ferradura/virologia
Nodaviridae/fisiologia
Peptídeos Cíclicos/metabolismo
Ranavirus/fisiologia
[Mh] Termos MeSH secundário: Animais
Peptídeos Catiônicos Antimicrobianos/genética
Proteínas de Artrópodes/genética
Proteínas de Ligação a DNA/genética
Caranguejos Ferradura/genética
Interferon Tipo I/genética
Interferon Tipo I/metabolismo
Peptídeos Cíclicos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (Arthropod Proteins); 0 (DNA-Binding Proteins); 0 (Interferon Type I); 0 (Peptides, Cyclic); 118231-04-2 (tachyplesin peptide, Tachypleus tridentatus)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170923
[Lr] Data última revisão:
170923
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160917
[St] Status:MEDLINE


  9 / 1424 MEDLINE  
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[PMID]:27594681
[Au] Autor:Godsey MH; Davulcu O; Nix JC; Skalicky JJ; Brüschweiler RP; Chapman MS
[Ad] Endereço:Department of Math/Science, Concordia University, Portland, OR 97211, USA.
[Ti] Título:The Sampling of Conformational Dynamics in Ambient-Temperature Crystal Structures of Arginine Kinase.
[So] Source:Structure;24(10):1658-1667, 2016 Oct 04.
[Is] ISSN:1878-4186
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arginine kinase provides a model for functional dynamics, studied through crystallography, enzymology, and nuclear magnetic resonance. Structures are now solved, at ambient temperature, for the transition state analog (TSA) complex. Analysis of quasi-rigid sub-domain displacements show that differences between the two TSA structures average about 5% of changes between substrate-free and TSA forms, and they are nearly co-linear. Small backbone hinge rotations map to sites that also flex on substrate binding. Anisotropic atomic displacement parameters (ADPs) are refined using rigid-body TLS constraints. Consistency between crystal forms shows that they reflect intrinsic molecular properties more than crystal lattice effects. In many regions, the favored directions of thermal/static displacement are appreciably correlated with movements on substrate binding. Correlation between ADPs and larger substrate-associated movements implies that the latter approximately follow paths of low-energy intrinsic motions.
[Mh] Termos MeSH primário: Arginina Quinase/química
Caranguejos Ferradura/enzimologia
[Mh] Termos MeSH secundário: Animais
Anisotropia
Cristalografia por Raios X
Caranguejos Ferradura/química
Modelos Moleculares
Ligação Proteica
Estrutura Secundária de Proteína
Estrutura Terciária de Proteína
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.7.3.3 (Arginine Kinase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160906
[St] Status:MEDLINE


  10 / 1424 MEDLINE  
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[PMID]:27494624
[Au] Autor:Fennrich S; Hennig U; Toliashvili L; Schlensak C; Wendel HP; Stoppelkamp S
[Ad] Endereço:Clinical Research Laboratory, Clinic of Thoracic, Cardiac and Vascular Surgery, University Hospital Tübingen, Germany.
[Ti] Título:More than 70 years of pyrogen detection: Current state and future perspectives.
[So] Source:Altern Lab Anim;44(3):239-53, 2016 Jul.
[Is] ISSN:0261-1929
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the quality assurance of medical products, tests for sterility are essential. For parenteral pharmaceuticals, avoiding the presence of pyrogens is crucial. These fever-inducing substances (endotoxins and non-endotoxins) are not eliminated by standard sterilisation processes, and are biologically active once in the bloodstream, causing risks to human health, ranging from mild reactions (e.g. fever) to septic shock and death. Therefore, for injectable formulations, pyrogen testing is mandatory. Over the years, various pyrogen testing methods have been introduced, namely: in the 1940s, the rabbit pyrogen test, which is an in vivo test that measures the fever reaction as an endpoint; in the 1970s, the Limulus Amoebocyte Lysate (LAL) test, which is an in vitro test (with the haemolymph of the horseshoe crab) that specifically detects endotoxin; and in 2010, the Monocyte-Activation Test (MAT), which is a non-animal based in vitro pyrogen test that represents a full replacement of the rabbit test. Due to the ubiquity and biological significance of pyrogens, we are currently further developing the MAT so that it can be used for other applications. More specifically, our focus is on the detection of pyrogenic contamination on medical devices, as well as on the measurement of air quality. In addition, further improvements to permit the use of cryopreserved blood in the MAT, to overcome the limitations in the availability of freshly-drawn blood from human donors, are ongoing.
[Mh] Termos MeSH primário: Alternativas aos Testes com Animais/métodos
Teste do Limulus/história
Pirogênios/isolamento & purificação
[Mh] Termos MeSH secundário: Alternativas aos Testes com Animais/tendências
Animais
Citocinas/genética
Citocinas/metabolismo
Regulação da Expressão Gênica/efeitos dos fármacos
História do Século XX
História do Século XXI
Caranguejos Ferradura/metabolismo
Seres Humanos
Monócitos/efeitos dos fármacos
Monócitos/metabolismo
Pirogênios/toxicidade
Coelhos
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cytokines); 0 (Pyrogens)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170107
[Lr] Data última revisão:
170107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160806
[St] Status:MEDLINE



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