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  1 / 20858 MEDLINE  
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[PMID]:29414994
[Au] Autor:Saavedra I; Amo L
[Ad] Endereço:Departamento de Ecología Evolutiva, Museo Nacional de Ciencias Naturales (CSIC), C/ José Gutiérrez Abascal, Madrid, Spain.
[Ti] Título:Insectivorous birds eavesdrop on the pheromones of their prey.
[So] Source:PLoS One;13(2):e0190415, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chemical cues play a fundamental role in mate attraction and mate choice. Lepidopteran females, such as the winter moth (Operophtera brumata), emit pheromones to attract males in the reproductive period. However, these chemical cues could also be eavesdropped by predators. To our knowledge, no studies have examined whether birds can detect pheromones of their prey. O. brumata adults are part of the winter diet of some insectivorous tit species, such as the great tit (Parus major) and blue tit (Cyanistes caeruleus). We performed a field experiment aimed to disentangle whether insectivorous birds can exploit the pheromones emitted by their prey for prey location. We placed artificial larvae and a dispenser on branches of Pyrenean oak trees (Quercus pyrenaica). In half of the trees we placed an O. brumata pheromone dispenser and in the other half we placed a control dispenser. We measured the predation rate of birds on artificial larvae. Our results show that more trees had larvae with signs of avian predation when they contained an O. brumata pheromone than when they contained a control dispenser. Furthermore, the proportion of artificial larvae with signs of avian predation was greater in trees that contained the pheromone than in control trees. Our results indicate that insectivorous birds can exploit the pheromones emitted by moth females to attract males, as a method of prey detection. These results highlight the potential use of insectivorous birds in the biological control of insect pests.
[Mh] Termos MeSH primário: Aves/fisiologia
Insetos
Feromônios/fisiologia
Comportamento Predatório
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Pheromones)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190415


  2 / 20858 MEDLINE  
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[PMID]:29351549
[Au] Autor:García-Ruiz E; Loureiro Í; Farinós GP; Gómez P; Gutiérrez E; Sánchez FJ; Escorial MC; Ortego F; Chueca MC; Castañera P
[Ad] Endereço:Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Departamento de Protección Vegetal, Laboratorio de Malherbología, Madrid, Spain.
[Ti] Título:Weeds and ground-dwelling predators' response to two different weed management systems in glyphosate-tolerant cotton: A farm-scale study.
[So] Source:PLoS One;13(1):e0191408, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The use of glyphosate, as a post-emergence broad-spectrum herbicide in genetically modified glyphosate-tolerant (GT) cotton, supposes a big change in weed management programs with respect to a conventional regime. Thus, alterations in arable flora and arthropod fauna must be considered when evaluating their potential impacts. A 3-year farm-scale study was conducted in a 2-ha GT cotton crop, in southern Spain, to compare the effects of conventional and glyphosate herbicide regimes on weed abundance and diversity and their consequences for ground-dwelling predators. Surveys reveal that weed density was relatively low within all treatments with a few dominant species, with significantly higher weed densities and modifications of the floristic composition in glyphosate-treated plots that led to an increase in the abundance of Portulaca oleracea and to a reduction in plant diversity. The activity-density of the main predatory arthropod taxa (spiders, ground beetles, rove beetles and earwigs) varied among years, but no significant differences were obtained between conventional and glyphosate herbicide regimes. However, significant differences between treatments were obtained for ground beetles species richness and diversity, being higher under the glyphosate herbicide regime, and a positive correlation with weed density could be established for both parameters. The implications of these findings to weed control in GT cotton are discussed.
[Mh] Termos MeSH primário: Glicina/análogos & derivados
Gossypium/efeitos dos fármacos
Herbicidas/farmacologia
Plantas Daninhas/efeitos dos fármacos
Controle de Plantas Daninhas/métodos
[Mh] Termos MeSH secundário: Animais
Artrópodes/efeitos dos fármacos
Biodiversidade
Coleópteros/efeitos dos fármacos
Produtos Agrícolas/efeitos dos fármacos
Produtos Agrícolas/genética
Produtos Agrícolas/crescimento & desenvolvimento
Ecossistema
Cadeia Alimentar
Glicina/farmacologia
Gossypium/genética
Gossypium/crescimento & desenvolvimento
Resistência a Herbicidas/genética
Insetos/efeitos dos fármacos
Plantas Geneticamente Modificadas
Espanha
Aranhas/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Herbicides); 4632WW1X5A (glyphosate); TE7660XO1C (Glycine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191408


  3 / 20858 MEDLINE  
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[PMID]:28747396
[Au] Autor:Vernerey F; Shen T
[Ad] Endereço:Mechanical Engineering, University of Colorado Boulder, 427 UCB, Boulder, CO, USA franck.vernerey@colorado.edu.
[Ti] Título:The mechanics of hydrogel crawlers in confined environment.
[So] Source:J R Soc Interface;14(132), 2017 Jul.
[Is] ISSN:1742-5662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We present theoretical and experimental results regarding the development of temperature-sensitive hydrogel particles that can display self-motility in confined channels. Inspired by the motility of living organisms such as larva, the motion of the particle relies on the combination of two key mechanisms. The first, referred to as actuation, is enabled by the cyclic extension and retraction of the particle owing to oscillations of its temperature around the so-called lower critical solution temperature. The second, referred to as symmetry breaking, transforms the isotropic particle actuation into a directed motion owing to the asymmetric friction properties of the channel's surface. The role of particle confinement in these processes is, however, less intuitive and displays an optimal value at which the particle's step size is maximum. These observations are supported by a model that identifies the underlying locomotion mechanisms and predicts the dependency of the particle motion efficiency on the confinement condition, as well as frictional properties of the substrate. Our analysis suggests that the existence of a lubrication layer around the particle hinders its motion at low confinement, while an excessive degree of confinement is detrimental to the particle's overall deformation and, thus, to its locomotion efficiency.
[Mh] Termos MeSH primário: Hidrogéis
Fenômenos Mecânicos
Modelos Teóricos
Movimento
[Mh] Termos MeSH secundário: Animais
Insetos/fisiologia
Larva/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrogels)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE


  4 / 20858 MEDLINE  
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[PMID]:29449477
[Au] Autor:Kremen C
[Ad] Endereço:Department of Environmental Science, Policy, and Management, University of California, Berkeley, Berkeley, CA 94720, USA. ckremen@berkeley.edu.
[Ti] Título:The value of pollinator species diversity.
[So] Source:Science;359(6377):741-742, 2018 02 16.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Biodiversidade
Polinização
[Mh] Termos MeSH secundário: Flores
Insetos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180217
[St] Status:MEDLINE
[do] DOI:10.1126/science.aar7614


  5 / 20858 MEDLINE  
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[PMID]:29401577
[Au] Autor:Ryss AY
[Ti] Título:The simplest "field" methods for extractin of nematodes from plants, wood, insects and soil, with additional description how to keep extracted nematodes alive for a long time.
[So] Source:Parazitologiia;51(1):57-67, 2017 Jan-Feb.
[Is] ISSN:0031-1847
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The simplest modification of the dynamic extraction method using cottonwool filter based on the Baermann funnel principle, is described. This modification excludes the funnel because a great share of Sticky worms attach to sloping walls of a funnel and thus do not reach the collector Eppendorf tube. But the main principle of the Baermann funnel is used, I. e. sinking down of actively moving heavy narrow bodies via wide holes of filter and thus separating the active worms from passive non-Brownian moving substrate particles, which do not pass the filter and remain above it. This principle is illustrated because it has never been described before. In the proposed modification any sloping walls in the extraction paths are excluded and thus the probability to attach sticky nemotodes to walls is also excluded; only cylindrical equipment with abrupt vertical walls is used; procedures are extremely simplified to be user-friendly for beginners: only filter (cotton pads), Eppendorf tubes, plastic glasses and narrow PVC tubing are applied. The new simplified modification allows one to collect nematodes by non-professional workers, e. g. in Polar expeditions without microscopic study of results. As an addition, an efficient method to maintain extracted nematodes alive is proposed, using the "effect of water film" in foam rubber inside the Eppendorf tube. To maintain nematodes alive during several months it is recommended to suppress bacteria via addition of 0.2-0.4% formaldehyde solution and then keep the tube with nematodes in a refrigerator.
[Mh] Termos MeSH primário: Desenho de Equipamento/instrumentação
Movimento/fisiologia
Nematoides/isolamento & purificação
Refrigeração/métodos
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
Fricção/fisiologia
Insetos/parasitologia
Plantas/parasitologia
Solo/parasitologia
Madeira/parasitologia
[Pt] Tipo de publicação:RESEARCH SUPPORT, NON-U.S. GOV'T; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180206
[St] Status:MEDLINE


  6 / 20858 MEDLINE  
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[PMID]:29315331
[Au] Autor:Invernizzi C; Nogueira E; Juri P; Santos E; Arredondo D; Branchiccela B; Mendoza Y; Antúnez K
[Ad] Endereço:Sección Etología, Instituto de Biología, Facultad de Ciencias, Montevideo, Uruguay.
[Ti] Título:Epormenis cestri secretions in Sebastiania schottiana trees cause mass death of honey bee Apis mellifera larvae in Uruguay.
[So] Source:PLoS One;13(1):e0190697, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:For more than 60 years, sporadic cases of massive summer honey bee larvae mortality in colonies located near freshwater systems with abundant riparian vegetation have been reported in Uruguay. This odd phenomenon, known as "River disease" by beekeepers, can lead to colony death by depopulation. The aim of this study was to detect the causes of larvae death. Different experiments and analyses were performed using affected apiaries located between two important water courses. 1 day old larvae were the most susceptible and substances that killed the larvae were present in the nectar but not in the pollen. A palynological analysis of nectar samples showed that bees collect this resource from commonly pollinated floral species in the country. However, abundant fungi spores and conidia were found, which indicates that the bees also collected honeydews. In the riparian vegetation, bees were observed collecting the secretions of the planthopper Epormenis cestri on Sebastiania schottiana trees. It was found that the mortality period of larvae overlaps with the presence of E. cestri. Larvae maintained in the laboratory were fed (i) nectar from healthy colonies, (ii) nectar from affected colonies, and (iii) secretions of E. cestri. The mortality of the larvae that received nectar from colonies affected with River disease and secretions of E. cestri was higher than the mortality of those receiving nectar from healthy colonies. This represents the first report of planthopper honeydew causing mass larval mortality in honey bees.
[Mh] Termos MeSH primário: Colapso da Colônia
Euphorbiaceae/química
Insetos/metabolismo
Larva
[Mh] Termos MeSH secundário: Animais
Uruguai
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190697


  7 / 20858 MEDLINE  
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[PMID]:29293646
[Au] Autor:Kiffer WP; Mendes F; Casotti CG; Costa LC; Moretti MS
[Ad] Endereço:Laboratory of Aquatic Insect Ecology, University of Vila Velha, Vila Velha, Brazil.
[Ti] Título:Exotic Eucalyptus leaves are preferred over tougher native species but affect the growth and survival of shredders in an Atlantic Forest stream (Brazil).
[So] Source:PLoS One;13(1):e0190743, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We evaluated the effect of leaves of native and exotic tree species on the feeding activity and performance of the larvae of Triplectides gracilis, a typical caddisfly shredder in Atlantic Forest streams. Leaves of four native species that differ in chemistry and toughness (Hoffmannia dusenii, Miconia chartacea, Myrcia lineata and Styrax pohlii) and the exotic Eucalyptus globulus were used to determine food preferences and rates of consumption, production of fine particulate organic matter (FPOM), growth and survival of shredders. We hypothesized that the consumption rates of leaves of Eucalyptus and their effects on the growth and survival of shredders could be predicted by leaf chemistry and toughness. The larvae preferred to feed on soft leaves (H. dusenii and M. chartacea) independently of the content of nutrients (N and P) and secondary compounds (total phenolics). When such leaves were absent, they preferred E. globulus and did not consume the tough leaves (M. lineata and S. pohlii). In monodietary experiments, leaf consumption and FPOM production differed among the studied leaves, and the values observed for the E. globulus treatments were intermediate between the soft and tough leaves. The larvae that fed on H. dusenii and M. chartacea grew constantly over five weeks, while those that fed on E. globulus lost biomass. Larval survival was higher on leaves of H. dusenii, M. chartacea and S. pohlii than on E. globulus and M. lineata leaves. Although E. globulus was preferred over tougher leaves, long-term consumption of leaves of the exotic species may affect the abundance of T. gracilis in the studied stream. Additionally, our results suggest that leaf toughness can be a determining factor for the behavior of shredders where low-quality leaves are abundant, as in several tropical streams.
[Mh] Termos MeSH primário: Eucalyptus
Comportamento Alimentar
Florestas
Insetos/fisiologia
Folhas de Planta
[Mh] Termos MeSH secundário: Animais
Brasil
Insetos/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190743


  8 / 20858 MEDLINE  
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[PMID]:28470614
[Au] Autor:Karste K; Bleckmann M; van den Heuvel J
[Ad] Endereço:Helmholtz Zentrum für Infektionsforschung GmbH, Inhoffenstrasse 7, 38124, Braunschweig, Germany.
[Ti] Título:Not Limited to E. coli: Versatile Expression Vectors for Mammalian Protein Expression.
[So] Source:Methods Mol Biol;1586:313-324, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recombinant protein expression is not limited to E. coli or other prokaryotic systems. It is inevitable to use eukaryotic systems in order to express challenging mammalian proteins. Eukaryotic systems are able to perform complex posttranslational modifications like protein processing, phosphorylation, glycosylation, which are essential for stability and functionality of many proteins. Different eukaryotic protein expression systems employing yeast, insect, or mammalian cell lines are established with each having its own advantages and disadvantages. Often it is quite difficult to decide which will be the most optimal expression system as this depends highly on the protein itself. Expression in stable cell lines requires substantial screening of expressible constructs prior to developing a stable expression cell line. To achieve fast screening by transient expression in multiple hosts, versatile vectors can be applied. In this chapter, we present an overview of the most common multi-host vectors, which allow for fast expression analysis without tedious (re)cloning of the gene of interest in several different protein production systems. The protocols in this chapter describe the latest methods for fast transient expression in insect and mammalian cell lines.
[Mh] Termos MeSH primário: Vetores Genéticos/genética
Proteínas Recombinantes/genética
Transfecção/métodos
[Mh] Termos MeSH secundário: Animais
Células CHO
Técnicas de Cultura de Células/métodos
Linhagem Celular
Clonagem Molecular/métodos
Cricetulus
Escherichia coli/genética
Expressão Gênica
Células HEK293
Seres Humanos
Insetos/citologia
Insetos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6887-9_20


  9 / 20858 MEDLINE  
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[PMID]:29297214
[Au] Autor:Diamandopoulos A
[Ti] Título:Medea's Nuptial Gifts: Myth and Biomedical Reality.
[So] Source:Vesalius;22(2 Suppl):14-25, 2016 Dec.
[Is] ISSN:1373-4857
[Cp] País de publicação:Belgium
[La] Idioma:eng
[Ab] Resumo:In all art forms, Medea is mainly represented as the tragic witch from Colchis (contemporary Georgia), who slaughtered her sons and killed her erotic rival Glauke and her father, King Creon of Corinth, by offering an elaborate poisonous nuptial garment. Euripides described the victims' symptoms as a sudden extreme inflammation, leading anyone coming into contact with the garment to death. In other version, the inflammation is described as pure fire. The symptoms resemble what current medical knowledge describes as an immune contact sensitivity reaction. The passages with medical interest from the opera based on this tragedy are presented in the original musical form as well as some similar film and theater scenes. Magnified images of harmful insect's Medea's nuptial gifts are shown and their action is discussed.
[Mh] Termos MeSH primário: Dermatite de Contato/história
Medicina nas Artes/história
Filmes Cinematográficos/história
Música/história
[Mh] Termos MeSH secundário: Animais
República da Geórgia
Doações
História Antiga
Insetos/fisiologia
Mitologia
Comportamento Sexual Animal
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:QIS
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE


  10 / 20858 MEDLINE  
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[PMID]:28468626
[Au] Autor:Xu Q; Liu H; Yuan P; Zhang X; Chen Q; Jiang X; Zhou Y
[Ad] Endereço:Institute of Plant Protection; Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing, Jiangsu, People's Republic of China.
[Ti] Título:Development of a simplified RT-PCR without RNA isolation for rapid detection of RNA viruses in a single small brown planthopper (Laodelphax striatellus Fallén).
[So] Source:Virol J;14(1):90, 2017 05 03.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The small brown planthopper (SBPH) is an important pest of cereal crops and acts as a transmission vector for multiple RNA viruses. Rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. Reverse transcription polymerase chain reaction (RT-PCR) is a rapid, sensitive and reliable method for virus detection. The traditional RT-PCR contains a RNA isolation step and is widely used for virus detection in insect. However, using the traditional RT-PCR for detecting RNA virus in individual SBPHs becomes challenging because of the expensive reagents and laborious procedure associated with RNA isolation when processing a large number of samples. RESULTS: We established a simplified RT-PCR method without RNA isolation for RNA virus detection in a single SBPH. This method is achieved by grinding a single SBPH in sterile water and using the crude extract directly as the template for RT-PCR. The crude extract containing the virus RNA can be prepared in approximately two minutes. Rice stripe virus (RSV), rice black streaked dwarf virus (RBSDV) and Himetobi P virus (HiPV) were successfully detected using this simplified method. The detection results were validated by sequencing and dot immunobinding assay, indicating that this simplified method is reliable for detecting different viruses in insects. The evaluation of the sensitivity of this method showed that both RSV and HiPV can be detected when the cDNA from the crude extract was diluted up to 10 fold. Compared to the traditional RT-PCR with RNA isolation, the simplified RT-PCR method greatly reduces the sample processing time, decreases the detection cost, and improves the efficiency by avoiding RNA isolation. CONCLUSIONS: A simplified RT-PCR method is developed for rapid detection of RNA virus in a single SBPH without the laborious RNA isolation step. It offers a convenient alternative to the traditional RT-PCR method.
[Mh] Termos MeSH primário: Hemípteros/virologia
Insetos Vetores/virologia
Vírus de RNA/isolamento & purificação
RNA Viral/isolamento & purificação
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
[Mh] Termos MeSH secundário: Animais
DNA Complementar/isolamento & purificação
Dicistroviridae/genética
Dicistroviridae/isolamento & purificação
Immunoblotting/métodos
Insetos/virologia
Vírus de Plantas/genética
Vírus de Plantas/isolamento & purificação
RNA Viral/análise
Reoviridae/genética
Reoviridae/isolamento & purificação
Sensibilidade e Especificidade
Tenuivirus/genética
Tenuivirus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (RNA, Viral)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-017-0732-6



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