Base de dados : MEDLINE
Pesquisa : B01.050.500.131.617.678.369 [Categoria DeCS]
Referências encontradas : 4130 [refinar]
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  1 / 4130 MEDLINE  
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[PMID]:29080673
[Au] Autor:Noguerales V; Cordero PJ; Ortego J
[Ad] Endereço:Grupo de Investigación de la Biodiversidad Genética y Cultural, Instituto de Investigación en Recursos Cinegéticos - IREC (CSIC, UCLM, JCCM), Ronda de Toledo 12, E-13071 Ciudad Real, Spain. Electronic address: victor.noguerales@csic.es.
[Ti] Título:Inferring the demographic history of an oligophagous grasshopper: Effects of climatic niche stability and host-plant distribution.
[So] Source:Mol Phylogenet Evol;118:343-356, 2018 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding the consequences of past environmental changes on the abiotic and biotic components of the landscape and deciphering their impacts on the demographic trajectories of species is a major issue in evolutionary biogeography. In this study, we combine nuclear and mitochondrial genetic data to study the phylogeographical structure and lineage-specific demographic histories of the scrub-legume grasshopper (Chorthippus binotatus binotatus), a montane taxon distributed in the Iberian Peninsula and France that exclusively feeds on certain scrub-legume species. Genetic data and paleo-distribution modelling indicate the presence of four main lineages that seem to have diverged in allopatry and long-term persisted in Iberian and French refugia since the Mid Pleistocene. Comparisons of different demographic hypotheses in an Approximate Bayesian Computation (ABC) framework supported a population bottleneck in the northwestern French clade and paleo-distribution modelling indicate that the populations of this lineage have experienced more severe environmental fluctuations during the last 21 000 years than those from the Iberian Peninsula. Accordingly, we found that nuclear genetic diversity of the populations of scrub-legume grasshopper is positively associated with local stability of suitable habitats defined by both Pleistocene climate changes and historical distributional shifts of host-plant species. Overall, our study highlights the importance of integrating the potential effects of abiotic (i.e. climate and geography) and biotic components (i.e. inter-specific interactions) into the study of the evolutionary and demographic history of specialist taxa with narrow ecological requirements.
[Mh] Termos MeSH primário: Variação Genética
Gafanhotos/genética
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
Mudança Climática
DNA/isolamento & purificação
DNA/metabolismo
Ecossistema
Complexo IV da Cadeia de Transporte de Elétrons/química
Complexo IV da Cadeia de Transporte de Elétrons/genética
França
Genética Populacional
Gafanhotos/classificação
Desequilíbrio de Ligação
Filogenia
Filogeografia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171030
[St] Status:MEDLINE


  2 / 4130 MEDLINE  
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[PMID]:28986236
[Au] Autor:Matenaar D; Fingerle M; Heym E; Wirtz S; Hochkirch A
[Ad] Endereço:Trier University, Department of Biogeography, D-54286 Trier, Germany; Stuttgart State Museum of Natural History, Department of Entomology, D-70191 Stuttgart, Germany; Hessisches Landesmuseum Darmstadt, Friedensplatz 1, D-64283 Darmstadt, Germany. Electronic address: daniela.matenaar@gmail.com.
[Ti] Título:Phylogeography of the endemic grasshopper genus Betiscoides (Lentulidae) in the South African Cape Floristic Region.
[So] Source:Mol Phylogenet Evol;118:318-329, 2018 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vicariance and dispersal are two important processes shaping biodiversity patterns. The South African Cape Floristic Region (CFR) is known for its high biotic diversity and endemism. However, studies on the phylogeography of endemic invertebrates in this biodiversity hotspot are still scarce. Here, we present a phylogenetic study of the flightless grasshopper genus Betiscoides, which is endemic to the CFR and strongly associated with restio plants (Restionaceae). We hypothesized that the genus originated in the southwestern part of the CFR, that differentiation within the genus is mainly an effect of vicariance and that the three known species only represent a minor fraction of the real genetic diversity of the genus. We inferred the phylogeny based on sequences of three mitochondrial and two nuclear genes from 99 Betiscoides specimens collected across the CFR. Furthermore, we conducted a SDIVA analysis to detect distributions of ancestral nodes and the possible spatial origin of these lineages. Strong differentiation among genetic lineages was shown. The ancestor of this genus was most likely distributed in the southwestern CFR. Five major lineages were detected, three of which were ancestrally distributed in the southwestern CFR. The ancestors of the two other lineages were distributed in the northern and eastern margins of the CFR. A total of 24 divergent evolutionary lineages were found, reflecting the geographical isolation of restio-dominated fynbos habitats. Dispersal played a more prominent role than expected in differentiation of Betiscoides. While the five main lineages were separated during a first phase via dispersal, differentiation occurred later and on smaller spatial scale, predominantly driven by isolation in montane refugia (i.e. vicariance). Our study also suggests that flightless insect taxa likely show high levels of differentiation in biodiversity hotspots with their taxonomy often being incomplete.
[Mh] Termos MeSH primário: Gafanhotos/classificação
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Teorema de Bayes
Evolução Biológica
DNA/química
DNA/isolamento & purificação
DNA/metabolismo
Variação Genética
Gafanhotos/genética
Mitocôndrias/genética
Filogenia
Filogeografia
Alinhamento de Sequência
Análise de Sequência de DNA
África do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171008
[St] Status:MEDLINE


  3 / 4130 MEDLINE  
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[PMID]:29284051
[Au] Autor:Köhler G; Samietz J; Schielzeth H
[Ad] Endereço:Population Ecology Group, Institute of Ecology, Friedrich Schiller University, Jena, Germany.
[Ti] Título:Morphological and colour morph clines along an altitudinal gradient in the meadow grasshopper Pseudochorthippus parallelus.
[So] Source:PLoS One;12(12):e0189815, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many animals show altitudinal clines in size, shape and body colour. Increases in body size and reduction in the length of body appendices in colder habitats are usually attributed to improved heat conservation at lower surface-to-volume ratios (known as Bergmann's and Allen's rule, respectively). However, the patterns are more variable and sometimes reversed in small ectotherms that are affected by shortened growing seasons. Altitude can also affect colouration. The thermal melanism hypothesis predicts darker colours under cooler conditions because of a thermoregulatory advantage. Darker colours may also be favoured at high altitudes for reasons of UV protection or habitat-dependent crypsis. We studied altitudinal variation in morphology and colour in the colour-polymorphic meadow grasshopper Pseudochorthippus parallelus based on 563 individuals from 17 populations sampled between 450 and 2,500 m asl. Pronotum length did not change with altitude, while postfemur length decreased significantly in both sexes. Tegmen (forewing) length decreased in males, but not in females. The results indicate that while body size, as best quantified by pronotum length, was remarkably constant, extended appendices were reduced at high altitudes. The pattern thus follows Allen's rule, but neither Bergmann's nor converse Bergmann's rule. These results indicate that inference of converse Bergmann's rule based on measurements from appendices should be treated with some caution. Colour morph ratios showed significant changes in both sexes from lowland populations dominated by green individuals to high-altitude populations dominated by brown ones. The increase of brown morphs was particularly steep between 1,500 and 2,000 m asl. The results suggest shared control of colour in males and females and local adaptation along the altitudinal gradient following the predictions of the thermal melanism hypothesis. Interestingly, both patterns, the reduction of body appendices and the higher frequency of brown individuals, may be explained by a need for efficient thermoregulation under high-altitude conditions.
[Mh] Termos MeSH primário: Altitude
Cor
Gafanhotos/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Gafanhotos/anatomia & histologia
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189815


  4 / 4130 MEDLINE  
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[PMID]:28745638
[Au] Autor:Xu X; You Y; Zhang L
[Ad] Endereço:Department of Entomology, China Agricultural University.
[Ti] Título:Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization.
[So] Source:J Vis Exp;(125), 2017 Jul 13.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Insects have evolved sophisticated olfactory reception systems to sense exogenous chemical signals. These chemical signals are transduced by Olfactory Receptor Neurons (ORNs) housed in hair-like structures, called chemosensilla, of the antennae. On the ORNs' membranes, Odorant Receptors (ORs) are believed to be involved in odor coding. Thus, being able to identify genes localized to the ORNs is necessary to recognize OR genes, and provides a fundamental basis for further functional in situ studies. The RNA expression levels of specific ORs in insect antennae are very low, and preserving insect tissue for histology is challenging. Thus, it is difficult to localize an OR to a specific type of sensilla using RNA in situ hybridization. In this paper, a detailed and highly effective RNA in situ hybridization protocol particularly for lowly expressed OR genes of insects, is introduced. In addition, a specific OR gene was identified by conducting double-color fluorescent in situ hybridization experiments using a co-expressing receptor gene, Orco, as a marker.
[Mh] Termos MeSH primário: Antenas de Artrópodes/patologia
Gafanhotos/metabolismo
RNA/metabolismo
Receptores Odorantes/genética
[Mh] Termos MeSH secundário: Animais
Antenas de Artrópodes/metabolismo
Imuno-Histoquímica
Hibridização in Situ Fluorescente
Microscopia Confocal
Neurônios Receptores Olfatórios/metabolismo
Neurônios Receptores Olfatórios/patologia
Oligonucleotídeos Antissenso/química
Oligonucleotídeos Antissenso/metabolismo
Receptores Odorantes/metabolismo
Gravação em Vídeo
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Nm] Nome de substância:
0 (Oligonucleotides, Antisense); 0 (Receptors, Odorant); 63231-63-0 (RNA)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.3791/55924


  5 / 4130 MEDLINE  
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[PMID]:28873574
[Au] Autor:Owens C; Griffin K; Khouryieh H; Williams K
[Ad] Endereço:Department of Chemistry, Western Kentucky University, Bowling Green, KY, USA.
[Ti] Título:Creaming and oxidative stability of fish oil-in-water emulsions stabilized by whey protein-xanthan-locust bean complexes: Impact of pH.
[So] Source:Food Chem;239:314-322, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The impact of pH on the physicochemical properties of 10% menhaden oil-in-water (O/W) emulsions containing 2% whey protein isolate (WPI) and 0.1% xanthan (XG)-locust bean gum (LBG) mixtures was investigated. The O/W emulsions containing 0.1% XG-LBG mixtures were compared to emulsions with 0.1% XG and 0.1% LBG. The results indicated that stability is dependent on pH and biopolymer type. At both pH 3 and 5, emulsions containing either XG or XG-LBG mixtures had large particle sizes, viscosity, droplet aggregation, and creaming index, resulting in poor physical stability which can be related to the adsorbed protein-polysaccharide interactions. At pH7, the XG-LBG emulsions showed the greatest resistance to phase separation and resulted in stable emulsions. Lipid oxidation measurements also indicated that XG-LBG mixtures can be used to form stable emulsions at pH 3 and pH 7. These results have significant implications for the development of novel structures containing lipid phases susceptible to lipid oxidation.
[Mh] Termos MeSH primário: Óleos de Peixe/química
[Mh] Termos MeSH secundário: Animais
Emulsões
Galactanos
Gafanhotos
Concentração de Íons de Hidrogênio
Mananas
Gomas Vegetais
Polissacarídeos Bacterianos
Água
Proteínas do Soro do Leite
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsions); 0 (Fish Oils); 0 (Galactans); 0 (Mannans); 0 (Plant Gums); 0 (Polysaccharides, Bacterial); 0 (Whey Proteins); 059QF0KO0R (Water); TTV12P4NEE (xanthan gum); V4716MY704 (locust bean gum)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  6 / 4130 MEDLINE  
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[PMID]:29023588
[Au] Autor:Huang X; Whitman DW; Ma J; McNeill MR; Zhang Z
[Ad] Endereço:State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
[Ti] Título:Diet alters performance and transcription patterns in Oedaleus asiaticus (Orthoptera: Acrididae) grasshoppers.
[So] Source:PLoS One;12(10):e0186397, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We reared Oedaleus asiaticus grasshoppers under four different single-plant diets to examine the relationships among diet, performance, stress, and transcription patterns. Grasshoppers fed only Artemisia frigida (Asteraceae) were stressed, as indicated by their lower growth, size, development, and survival, in comparison to grasshoppers fed on any of three grasses, Cleistogenes squarrosa, Leymus chinensis, or Stipa krylovii (all Poaceae). We then used transcriptome analysis to examine how gene expression levels in O. asiaticus were altered by feeding on these diets. Nymphs fed A. frigida had the largest variation in gene expression profiles with a total of 299 genes significantly up- or down-regulated compared to those feeding on the three grasses: down-regulated genes included those involved in cuticle biosynthesis, DNA replication, biosynthesis and metabolism of nutrition. The up-regulated genes included stress-resistant and detoxifying enzymes. GO and KEGG enrichment analysis also showed that feeding on A. frigida could down-regulate biosynthesis and metabolism related pathways, and up-regulate stress-resistant and detoxification terms and pathways. Our results show that diet significantly altered gene-expression, and that unfavorable, stressful diets induce more transcriptional changes than favorable diets. Altered gene-expression represents phenotypic plasticity, and many such changes appear to be evolved, adaptive responses. The ease and regularity by which individuals shift phenotypes via altered transcription suggests that populations consist not of similar, fixed phenotypes, but of a collection of ever-changing, divergent phenotypes.
[Mh] Termos MeSH primário: Gafanhotos/genética
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Análise por Conglomerados
Regulação para Baixo
Comportamento Alimentar
Gafanhotos/fisiologia
Ninfa/metabolismo
Poaceae/crescimento & desenvolvimento
RNA/química
RNA/isolamento & purificação
RNA/metabolismo
Análise de Sequência de DNA
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
63231-63-0 (RNA)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186397


  7 / 4130 MEDLINE  
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[PMID]:28982393
[Au] Autor:Sun Y; Liu D; Xiao B; Jiang G
[Ad] Endereço:School of Biology and Basic Medical Sciences, Medical College, Soochow University, 199 Ren'ai Road, Suzhou, 215123, Jiangsu, China.
[Ti] Título:The comparative mitogenomics and phylogenetics of the two grouse-grasshoppers (Insecta, Orthoptera, Tetrigoidea).
[So] Source:Biol Res;50(1):34, 2017 Oct 05.
[Is] ISSN:0717-6287
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: This study aimed to reveal the mitochondrial genomes (mtgenomes) of Tetrix japonica and Alulatettix yunnanensis, and the phylogenetics of Orthoptera species. METHODS: The mtgenomes of A. yunnanensis and T. japonica were firstly sequenced and assembled through partial sequences amplification, and then the genome organization and gene arrangement were analyzed. Based on nucleotide/amino acid sequences of 13 protein-coding genes and whole mtgenomes, phylogenetic trees were established on 37 Orthoptera species and 5 outgroups, respectively. RESULTS: Except for a regulation region (A+T rich region), a total of 37 genes were found in mtgenomes of T. japonica and A. yunnanensis, including 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes, which exhibited similar characters with other Orthoptera species. Phylogenetic tree based on 13 concatenated protein-coding nucleotide sequences were considered to be more suitable for phylogenetic reconstruction of Orthoptera species than amino acid sequences and mtgenomes. The phylogenetic relationships of Caelifera species were Acridoidea and Pamphagoidea > Pyrgomorphoidea > Pneumoroidea > Eumastacoidea > Tetrigoidea > Tridactyloidea. Besides, a sister-group relationship between Tettigonioidea and Rhaphidophoroidea was revealed in Ensifera. CONCLUSION: Concatenated protein-coding nucleotide sequences of 13 genes were suitable for reconstruction of phylogenetic relationship in orthopteroid species. Tridactyloidea was a sister group of Tetrigoidea in Caelifera, and Rhaphidophoroidea was a sister group of Tettigonioidea in Ensifera.
[Mh] Termos MeSH primário: Evolução Molecular
Genoma Mitocondrial/genética
Gafanhotos/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Gafanhotos/classificação
Filogenia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1186/s40659-017-0132-9


  8 / 4130 MEDLINE  
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[PMID]:28851268
[Au] Autor:Milani D; Ramos É; Loreto V; Martí DA; Cardoso AL; de Moraes KCM; Martins C; Cabral-de-Mello DC
[Ad] Endereço:Departamento de Biologia, UNESP - Univ Estadual Paulista, Instituto de Biociências/IB, Rio Claro, São Paulo, CEP 13506-900, Brazil.
[Ti] Título:The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata.
[So] Source:BMC Genet;18(1):81, 2017 Aug 29.
[Is] ISSN:1471-2156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Satellite DNAs (satDNAs) are organized in repetitions directly contiguous to one another, forming long arrays and composing a large portion of eukaryote genomes. These sequences evolve according to the concerted evolution model, and homogenization of repeats is observed at the intragenomic level. Satellite DNAs are the primary component of heterochromatin, located primarily in centromeres and telomeres. Moreover, satDNA enrichment in specific chromosomes has been observed, such as in B chromosomes, that can provide clues about composition, origin and evolution of this chromosome. In this study, we isolated and characterized a satDNA in A and B chromosomes of Abracris flavolineata by integrating cytogenetic, molecular and genomics approaches at intra- and inter-population levels, with the aim to understand the evolution of satDNA and composition of B chromosomes. RESULTS: AflaSAT-1 satDNA was shared with other species and in A. flavolineata, was associated with another satDNA, AflaSAT-2. Chromosomal mapping revealed centromeric blocks variable in size in almost all chromosomes (except pair 11) of A complement for both satDNAs, whereas for B chromosome, only a small centromeric signal occurred. In distinct populations, variable number of AflaSAT-1 chromosomal sites correlated with variability in copy number. Instead of such variability, low sequence diversity was observed in A complement, but monomers from B chromosome were more variable, presenting also exclusive mutations. AflaSAT-1 was transcribed in five tissues of adults in distinct life cycle phases. CONCLUSIONS: The sharing of AflaSAT-1 with other species is consistent with the library hypothesis and indicates common origin in a common ancestor; however, AflaSAT-1 was highly amplified in the genome of A. flavolineata. At the population level, homogenization of repeats in distinct populations was documented, but dynamic expansion or elimination of repeats was also observed. Concerning the B chromosome, our data provided new information on the composition in A. flavolineata. Together with previous results, the sequences of heterochromatic nature were not likely highly amplified in the entire B chromosome. Finally, the constitutive transcriptional activity suggests a possible unknown functional role, which should be further investigated.
[Mh] Termos MeSH primário: Cromossomos de Insetos
DNA Satélite
Gafanhotos/genética
[Mh] Termos MeSH secundário: Animais
Mapeamento Cromossômico
Variações do Número de Cópias de DNA
Evolução Molecular
Genômica
Hibridização in Situ Fluorescente
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Satellite)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1186/s12863-017-0548-9


  9 / 4130 MEDLINE  
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[PMID]:28752327
[Au] Autor:Boyan G; Liu Y; Khalsa SK; Hartenstein V
[Ad] Endereço:Graduate School of Systemic Neuroscience, Biocenter, Ludwig-Maximilians-Universität, Grosshadernerstrasse 2, 82152, Planegg-Martinsried, Germany.
[Ti] Título:A conserved plan for wiring up the fan-shaped body in the grasshopper and Drosophila.
[So] Source:Dev Genes Evol;227(4):253-269, 2017 Jul.
[Is] ISSN:1432-041X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The central complex comprises an elaborate system of modular neuropils which mediate spatial orientation and sensory-motor integration in insects such as the grasshopper and Drosophila. The neuroarchitecture of the largest of these modules, the fan-shaped body, is characterized by its stereotypic set of decussating fiber bundles. These are generated during development by axons from four homologous protocerebral lineages which enter the commissural system and subsequently decussate at stereotypic locations across the brain midline. Since the commissural organization prior to fan-shaped body formation has not been previously analyzed in either species, it was not clear how the decussating bundles relate to individual lineages, or if the projection pattern is conserved across species. In this study, we trace the axonal projections from the homologous central complex lineages into the commissural system of the embryonic and larval brains of both the grasshopper and Drosophila. Projections into the primordial commissures of both species are found to be lineage-specific and allow putatively equivalent fascicles to be identified. Comparison of the projection pattern before and after the commencement of axon decussation in both species reveals that equivalent commissural fascicles are involved in generating the columnar neuroarchitecture of the fan-shaped body. Further, the tract-specific columns in both the grasshopper and Drosophila can be shown to contain axons from identical combinations of central complex lineages, suggesting that this columnar neuroarchitecture is also conserved.
[Mh] Termos MeSH primário: Drosophila/crescimento & desenvolvimento
Gafanhotos/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Axônios/metabolismo
Evolução Biológica
Padronização Corporal
Encéfalo/citologia
Drosophila/citologia
Drosophila/genética
Drosophila/metabolismo
Gafanhotos/citologia
Gafanhotos/genética
Gafanhotos/metabolismo
Larva/crescimento & desenvolvimento
Larva/metabolismo
Neurônios
Neurópilo/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170810
[Lr] Data última revisão:
170810
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170729
[St] Status:MEDLINE
[do] DOI:10.1007/s00427-017-0587-2


  10 / 4130 MEDLINE  
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[PMID]:28733163
[Au] Autor:Sugahara R; Tanaka S; Shiotsuki T
[Ad] Endereço:National Agriculture and Food Research Organization, Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634, Japan. Electronic address: rsugahara@affrc.go.jp.
[Ti] Título:RNAi-mediated knockdown of SPOOK reduces ecdysteroid titers and causes precocious metamorphosis in the desert locust Schistocerca gregaria.
[So] Source:Dev Biol;429(1):71-80, 2017 09 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Halloween gene SPOOK (SPO) is involved in the production of the active metabolite of ecdysteroid, 20-hydroxyecdysone (20E), in insects. A previous study showed that RNAi-mediated knockdown of SPO in Schistocerca gregaria last instar nymphs markedly reduced the hemolymph 20E titer, but did not affect metamorphosis. In the present study, the effects of SPO interference on development were re-examined in this locust. Injections of SPO double-stranded RNA (dsSPO) into nymphs at mid and late instars significantly delayed nymphal development and interfered with molting. The 20E levels of dsSPO-treated nymphs were generally low, with a delayed, small peak, suggesting that disturbance of the 20E levels caused the above developmental abnormalities. A small proportion of the dsSPO-injected nymphs metamorphosed precociously, producing adults and adultoids. Precocious adults were characterized by small body size, short wings with abbreviated venation, and normal reproductive activity. Fourth instar nymphs that precociously metamorphosed at the following instar exhibited temporal expression patterns of ecdysone-induced protein 93F and the juvenile hormone (JH) early-inducible gene Krüppel homolog 1 similar to those observed at the last instar in normal nymphs. Adultoids displayed mating behavior and adultoid females developed eggs, but never laid eggs. JH injection around the expected time of the 20E peak in the dsSPO-injected nymphs completely inhibited the appearance of adultoids, suggesting that appearance of adultoids might be due to a reduced titer of JH rather than of 20E. These results suggest that SPO plays an important role in controlling morphogenesis, metamorphosis, and reproduction in S. gregaria.
[Mh] Termos MeSH primário: Clima Desértico
Ecdisteroides/metabolismo
Técnicas de Silenciamento de Genes
Gafanhotos/crescimento & desenvolvimento
Gafanhotos/genética
Proteínas de Insetos/genética
Metamorfose Biológica
Interferência de RNA
[Mh] Termos MeSH secundário: Animais
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Gafanhotos/efeitos dos fármacos
Hemolinfa/metabolismo
Proteínas de Insetos/metabolismo
Hormônios Juvenis/administração & dosagem
Hormônios Juvenis/farmacologia
Larva/efeitos dos fármacos
Metamorfose Biológica/efeitos dos fármacos
Metamorfose Biológica/genética
Muda/efeitos dos fármacos
RNA de Cadeia Dupla/metabolismo
Asas de Animais/efeitos dos fármacos
Asas de Animais/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ecdysteroids); 0 (Insect Proteins); 0 (Juvenile Hormones); 0 (RNA, Double-Stranded)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170723
[St] Status:MEDLINE



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