Base de dados : MEDLINE
Pesquisa : B01.050.500.131.617.678.369.500 [Categoria DeCS]
Referências encontradas : 305 [refinar]
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[PMID]:29401571
[Au] Autor:Kryukov VY; Rotskaya UN; Yaroslavtseva ON; Elisaphenko EA; Duisembekov BA; Glupov VV
[Ti] Título:[Phenotypic and genetic changes of entomopathogenic ascomycete Beauveria Bassiana under passaging through various hosts].
[So] Source:Parazitologiia;51(1):3-14, 2017 Jan-Feb.
[Is] ISSN:0031-1847
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Phenotypic and genetic estimations of entomopathogenic ascomycete B.bassiana (strain Sar-31) after 6-passaging through four hosts were shown. Increasing of virulence, changes in morpho-cultural characteristics and variations in Inter Simple Sequence Repeats (ISSR) assay between initial and reisolated cultures were registered. Six passages of entomopathogenic ascomycete Beauveria bassiana (strain Sar-31) through four hosts (Galleria mellonella, Tenebrio molitor, Leptinotarsa decemlineata, Locusta migratoria) and following estimation of phenotypic and genetic differences of the initial strain and reisolated cultures were conducted. The passaging of strain through certain host led to increasing of virulence for both this host and other test-insects. Unidirectional changes of morpho-cultural characteristics: colonies pigmentation and relief strengthening, increasing of conidia production and lipolytic activity were registered in all passaged cultures. Genetic analysis with 6 ISSR markers revealed variations between initial and reisolated cultures in 3 markers. Taken together, the results of this study help us understand potential ways of fungi strains changes during epizootic process and possibilities of ISSR assay applying for investigation of pathogen transmission.
[Mh] Termos MeSH primário: Beauveria/genética
Beauveria/patogenicidade
Proteínas Fúngicas/genética
Especificidade de Hospedeiro
Esporos Fúngicos/genética
Esporos Fúngicos/patogenicidade
[Mh] Termos MeSH secundário: Animais
Beauveria/enzimologia
Beauveria/crescimento & desenvolvimento
Coleópteros/microbiologia
Proteínas Fúngicas/metabolismo
Marcadores Genéticos
Genótipo
Lipólise
Locusta migratoria/microbiologia
Repetições de Microssatélites
Mariposas/microbiologia
Fenótipo
Pigmentação/genética
Inoculações Seriadas
Esporos Fúngicos/enzimologia
Esporos Fúngicos/crescimento & desenvolvimento
Tenebrio/microbiologia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Genetic Markers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180206
[St] Status:MEDLINE


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[PMID]:28452426
[Au] Autor:Chen B; Ma R; Ding D; Wei L; Kang L
[Ad] Endereço:State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
[Ti] Título:Aerobic respiration by haemocyanin in the embryo of the migratory locust.
[So] Source:Insect Mol Biol;26(4):461-468, 2017 08.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:It remains unresolved how insect embryos acquire sufficient oxygen to sustain high rates of respiratory metabolism during embryogenesis in the absence of a fully developed tracheal system. Our previous work showed that the two distinct subunits (Hc1 and Hc2) of haemocyanin (Hc), a copper-containing protein, display embryo-specific high expression that is essential for embryonic development and survival in the migratory locust Locusta migratoria. Here we investigated the role of haemocyanins in oxygen sensing and supply in the embryo of this locust. Putative binding sites for hypoxia-regulated transcription factors were identified in the promoter region of all of the Hc1 and Hc2 genes. Embryonic expression of haemocyanins was highly upregulated by ambient O deprivation, up to 10-fold at 13% O content. The degree of upregulation of haemocyanins increased with increasing levels of hypoxia. Compared with low-altitude locusts, embryonic expression of haemocyanins in high-altitude locusts from Tibetan plateau was constitutively higher and more robust to oxygen deprivation. These findings strongly suggest an active involvement of haemocyanins in oxygen exchange in embryos. We thus propose a mechanistic model for embryo respiration in which haemocyanin plays a key role by complementing the tracheal system for oxygen transport during embryogenesis.
[Mh] Termos MeSH primário: Hemocianinas/metabolismo
Locusta migratoria/embriologia
[Mh] Termos MeSH secundário: Animais
Respiração Celular
Embrião não Mamífero/metabolismo
Regulação da Expressão Gênica
Hipóxia/metabolismo
Locusta migratoria/metabolismo
Oxigênio/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
9013-72-3 (Hemocyanins); S88TT14065 (Oxygen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12310


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[PMID]:28988146
[Au] Autor:Li H; Xia Y
[Ad] Endereço:The Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418008, China; Genetic Engineering Research Center, College of Life Sciences, Chongqing University, Chongqing 400030, China.
[Ti] Título:Recombinant production of the insecticidal scorpion toxin BjαIT in Escherichia coli.
[So] Source:Protein Expr Purif;142:62-67, 2018 Feb.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Scorpion long-chain insect neurotoxins have important potential application value in agricultural pest control. The difficulty of obtaining natural toxins is the major obstacle preventing analyses of their insecticidal activity against more agricultural insect pests. Here we cloned the insect neurotoxin BjαIT gene into the pET32 expression vector and expressed the resulting thioredoxin (Trx)-BjαIT fusion protein in Escherichia coli. Soluble Trx-BjαIT was expressed at a high level when induced at 18 °C with 0.1 mM isopropyl ß-d-1-thiogalactopyranoside, and it was purified by Ni -nitriloacetic acid affinity chromatography. After cleaving the Trx tag with recombinant enterokinase, the digestion products were purified by CM Sepharose FF ion-exchange chromatography, and 1.5 mg of purified recombinant BjαIT (rBjαIT) was obtained from 100 ml of induced bacterial cells. Injecting rBjαIT induced obvious neurotoxic symptoms and led to death in locust (Locusta migratoria) larvae. Dietary toxicity was not observed in locusts. The results demonstrate that active rBjαIT could be obtained efficiently from an E. coli expression system, which is helpful for determining its insecticidal activity against agricultural insect pests.
[Mh] Termos MeSH primário: Larva/efeitos dos fármacos
Locusta migratoria/efeitos dos fármacos
Proteínas Recombinantes de Fusão/biossíntese
Venenos de Escorpião/biossíntese
Escorpiões/química
[Mh] Termos MeSH secundário: Animais
Cromatografia por Troca Iônica/métodos
Clonagem Molecular
Enteropeptidase/química
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Inseticidas/isolamento & purificação
Inseticidas/metabolismo
Inseticidas/toxicidade
Isopropiltiogalactosídeo/farmacologia
Larva/fisiologia
Locusta migratoria/fisiologia
Plasmídeos/química
Plasmídeos/metabolismo
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/isolamento & purificação
Proteínas Recombinantes de Fusão/toxicidade
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Venenos de Escorpião/genética
Venenos de Escorpião/isolamento & purificação
Venenos de Escorpião/toxicidade
Solubilidade
Tiorredoxinas/genética
Tiorredoxinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 0 (Recombinant Fusion Proteins); 0 (Recombinant Proteins); 0 (Scorpion Venoms); 0 (alphaIT toxin, Buthotus judaicus); 367-93-1 (Isopropyl Thiogalactoside); 52500-60-4 (Thioredoxins); EC 3.4.21.9 (Enteropeptidase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


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[PMID]:28576656
[Au] Autor:Song H; Zhang J; Li D; Cooper AMW; Silver K; Li T; Liu X; Ma E; Zhu KY; Zhang J
[Ad] Endereço:Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China; College of Life Science, Shanxi University, Taiyuan, Shanxi 030006, China.
[Ti] Título:A double-stranded RNA degrading enzyme reduces the efficiency of oral RNA interference in migratory locust.
[So] Source:Insect Biochem Mol Biol;86:68-80, 2017 Jul.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Application of RNA interference (RNAi) for insect pest management is limited by variable efficiency of RNAi in different insect species. In Locusta migratoria, RNAi is highly efficient through injection of dsRNA, but oral delivery of dsRNA is much less effective. Efforts to understand this phenomenon have shown that dsRNA is more rapidly degraded in midgut fluid than in hemolymph due to nuclease enzyme activity. In the present study, we identified and characterized two full-length cDNAs of double-stranded RNA degrading enzymes (dsRNase) from midgut of L. migratoria, which were named LmdsRNase2 and LmdsRNase3. Gene expression analysis revealed that LmdsRNase2 and LmdsRNase3 were predominantly expressed in the midgut, relatively lower expression in gastric caeca, and trace expression in other tested tissues. Incubation of dsRNA in midgut fluid from LmdsRNase3-suppressed larvae or control larvae injected with dsGFP resulted in high levels of degradation; however, dsRNA incubated in midgut fluid from LmdsRNase2-suppressed larvae was more stable, indicating LmdsRNase2 is responsible for dsRNA degradation in the midgut. To verify the biological function of LmdsRNase2 in vivo, nymphs were injected with dsGFP, dsLmdsRNase2 or dsLmdsRNase3 and chitinase 10 (LmCht10) or chitin synthase 1 (LmCHS1) dsRNA were orally delivered. Mortality associated with reporter gene knockdown was observed only in locusts injected with dsLmdsRNase2 (48% and 22%, for dsLmCht10 and dsLmCHS1, respectively), implicating LmdsRNase2 in reducing RNAi efficiency. Furthermore, recombinantly expressed LmdsRNase2 fusion proteins degraded dsRNA rapidly, whereas LmdsRNase3 did not. These results suggest that rapid degradation of dsRNA by dsRNase2 in the midgut is an important factor causing low RNAi efficiency when dsRNA is orally delivered in the locust.
[Mh] Termos MeSH primário: Locusta migratoria/enzimologia
Interferência de RNA
RNA de Cadeia Dupla/metabolismo
Ribonucleases/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas de Insetos/metabolismo
Dados de Sequência Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (RNA, Double-Stranded); EC 3.1.- (Ribonucleases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170604
[St] Status:MEDLINE


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[PMID]:28356351
[Au] Autor:Luo M; Li D; Wang Z; Guo W; Kang L; Zhou S
[Ad] Endereço:From the State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101.
[Ti] Título:Juvenile hormone differentially regulates two genes encoding protein chaperones required for insect fat body cell homeostasis and vitellogenesis.
[So] Source:J Biol Chem;292(21):8823-8834, 2017 May 26.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Juvenile hormone (JH) has a well known role in stimulating insect vitellogenesis ( yolk deposition) and oocyte maturation, but the molecular mechanisms of JH action in insect reproduction are unclear. The 78-kDa glucose-regulated protein (Grp78) is a heat shock protein 70-kDa family member and one of the most abundant chaperones in the endoplasmic reticulum (ER) where it helps fold newly synthesized peptides. Because of its prominent role in protein folding, and also ER stress, we hypothesized that might be involved in fat body cell homeostasis and vitellogenesis and a regulatory target of JH. We report here that the migratory locust possesses two genes that are differentially regulated by JH. We found that is regulated by JH through Mcm4/7-dependent DNA replication and polyploidization, whereas expression is directly activated by the JH-receptor complex comprising methoprene-tolerant and Taiman proteins. Interestingly, expression in the fat body is about 10-fold higher than that of Knockdown of either or significantly reduced levels of vitellogenin (Vg) protein, accompanied by retarded maturation of oocytes. Depletion of both and resulted in ER stress and apoptosis in the fat body and in severely defective Vg synthesis and oocyte maturation. These results indicate a crucial role of Grp78 in JH-dependent vitellogenesis and egg production. The presence and differential regulation of two genes in likely help accelerate the production of this chaperone in the fat body to facilitate folding of massively synthesized Vg and other proteins.
[Mh] Termos MeSH primário: Corpo Adiposo/metabolismo
Proteínas de Choque Térmico/biossíntese
Hormônios Juvenis/metabolismo
Locusta migratoria/metabolismo
Vitelogênese/fisiologia
Vitelogeninas/biossíntese
[Mh] Termos MeSH secundário: Animais
Feminino
Técnicas de Silenciamento de Genes
Proteínas de Choque Térmico/genética
Hormônios Juvenis/genética
Locusta migratoria/genética
Oócitos/metabolismo
Vitelogeninas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Heat-Shock Proteins); 0 (Juvenile Hormones); 0 (Vitellogenins); 0 (molecular chaperone GRP78)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170609
[Lr] Data última revisão:
170609
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.780957


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[PMID]:28339104
[Au] Autor:Osimani A; Garofalo C; Aquilanti L; Milanovic V; Cardinali F; Taccari M; Pasquini M; Tavoletti S; Clementi F
[Ad] Endereço:Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
[Ti] Título:Transferable Antibiotic Resistances in Marketed Edible Grasshoppers (Locusta migratoria migratorioides).
[So] Source:J Food Sci;82(5):1184-1192, 2017 May.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Grasshoppers are the most commonly eaten insects by humans worldwide, as they are rich in proteins and micronutrients. This study aimed to assess the occurrence of transferable antibiotic resistance genes in commercialized edible grasshoppers. To this end, the prevalence of 12 selected genes [aac(6')-Ie aph(2″)-Ia, blaZ, erm(A), erm(B), erm(C), mecA, tet(M), tet(O), tet(S), tet(K), vanA, vanB] coding for resistance to antibiotics conventionally used in clinical practice was determined. The majority of samples were positive for tet(M) (70.0%), tet(K) (83.3%) and blaZ (83.3%). A low percentage of samples were positive for erm(B) (16.7%), erm(C) (26.7%), and aac(6')-Ie aph(2″)-Ia (13.3%), whereas no samples were positive for erm(A), vanA, vanB, tet(O), and mecA. Cluster analysis identified 4 main clusters, allowing a separation of samples on the basis of their country of origin.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bactérias/efeitos dos fármacos
Farmacorresistência Bacteriana
Microbiologia de Alimentos
Locusta migratoria/microbiologia
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13700


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[PMID]:28257837
[Au] Autor:Redeker J; Bläser M; Neupert S; Predel R
[Ad] Endereço:Institute for Zoology, Functional Peptidomics Group, University of Cologne, D-50674 Cologne, Zuelpicher Str. 47b, Germany.
[Ti] Título:Identification and distribution of products from novel tryptopyrokinin genes in the locust, Locusta migratoria.
[So] Source:Biochem Biophys Res Commun;486(1):70-75, 2017 Apr 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A recent analysis of the genome of Locusta migratoria indicated the presence of four novel insect neuropeptide genes encoding for multiple tryptopyrokinin peptides (tryptoPKs); hitherto only known from pyrokinin or capa genes. In our study, mature products of tryptoPK genes 1 and 2 were identified by mass spectrometry; precursor sequences assigned to the tryptoPK genes 3 and 4 are likely partial sequences of a single precursor. The expression of tryptoPK genes 1 and 2 is restricted to two cells in the subesophageal ganglion, exhibiting not only a unique neuropeptidome but also a very distinctive axonal projection. Comparative neuroendocrinology revealed that homologous cells in other insects also produce tryptoPKs but use other genes to generate this pattern. Since capa and pyrokinin genes are discussed as ancestors of the tryptoPK genes, we completed the hitherto only partially known precursor sequences of these genes by means of transcriptome analyses. The distribution of mature products of CAPA and pyrokinin precursors in the CNS is compared with that of tryptoPKs. In addition, a novel pyrokinin-like precursor is described.
[Mh] Termos MeSH primário: Proteínas de Insetos/genética
Locusta migratoria/genética
Família Multigênica/genética
Neuropeptídeos/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sistema Nervoso Central/metabolismo
Esôfago/inervação
Gânglios dos Invertebrados/citologia
Gânglios dos Invertebrados/metabolismo
Perfilação da Expressão Gênica/métodos
Imuno-Histoquímica
Proteínas de Insetos/metabolismo
Locusta migratoria/metabolismo
Microscopia Confocal
Neurônios/metabolismo
Neuropeptídeos/metabolismo
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
Precursores de Proteínas/genética
Precursores de Proteínas/metabolismo
Proteômica/métodos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Neuropeptides); 0 (Protein Isoforms); 0 (Protein Precursors); 0 (pyrokinin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE


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[PMID]:28237581
[Au] Autor:Cross KP; Britton S; Mangulins R; Money TG; Robertson RM
[Ad] Endereço:Centre for Neuroscience Studies, Queen's University, Kingston, Ontario K7L 3N6, Canada. Electronic address: 13kc18@queensu.ca.
[Ti] Título:Food deprivation and prior anoxic coma have opposite effects on the activity of a visual interneuron in the locust.
[So] Source:J Insect Physiol;98:336-346, 2017 Apr.
[Is] ISSN:1879-1611
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We compared how different metabolic stressors, anoxic coma and food deprivation, affected signaling in neural tissue. We used the locust's Descending Contralateral Movement Detector (DCMD) interneuron because its large axon, high firing frequencies, and rapid conduction velocity make it energetically expensive. We exposed locusts to a 30min anoxic coma or 1day of food deprivation and found contrasting effects on signaling within the axon. After a prior anoxic coma, the DCMD fired fewer high-frequency (>200Hz) action potentials (APs) (Control: 12.4±1.6; Coma: 6.3±0.9) with a reduction in axonal conduction velocity (CV) at all frequencies (∼4-8%) when presented with a standard looming visual stimulus. Prior anoxic coma was also associated with a loss of supernormal conduction by reducing both the number of supernormal APs and the firing frequency with the highest CV. Initially, food deprivation caused a significant increase in the number of low- and high-frequency APs with no differences observed in CV. After controlling for isolation, food deprivation resulted in an increase in high-frequency APs (>200Hz: Control: 17.1±1.7; Food-deprived: 19.9±1.3) and an increase in relative conduction velocity for frequencies >150Hz (∼2%). Action potentials of food-deprived animals had a smaller half-width (Control: 0.45±0.02ms; Food-deprived: 0.40±0.01ms) and decay time (Control: 0.62±0.03ms; Food-deprived: 0.54±0.02ms). Our data indicate that the effects of metabolic stress on neural signaling can be stressor-dependent.
[Mh] Termos MeSH primário: Potenciais de Ação
Interneurônios/fisiologia
Locusta migratoria/fisiologia
Percepção Visual
[Mh] Termos MeSH secundário: Anaerobiose
Animais
Privação de Alimentos
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE


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[PMID]:28150067
[Au] Autor:Wasser H; Biller A; Antonopoulos G; Meyer H; Bicker G; Stern M
[Ad] Endereço:Division of Cell Biology, University of Veterinary Medicine Hannover, Bischofsholer Damm 15/102, D-30173, Hannover, Germany.
[Ti] Título:Regeneration of axotomized olfactory neurons in young and adult locusts quantified by fasciclin I immunofluorescence.
[So] Source:Cell Tissue Res;368(1):1-12, 2017 Apr.
[Is] ISSN:1432-0878
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The olfactory pathway of the locust Locusta migratoria is characterized by a multiglomerular innervation of the antennal lobe (AL) by olfactory receptor neurons (ORNs). After crushing the antenna and thereby severing ORN axons, changes in the AL were monitored. First, volume changes were measured at different times post-crush with scanning laser optical tomography in 5th instar nymphs. AL volume decreased significantly to a minimum volume at 4 days post-crush, followed by an increase. Second, anterograde labeling was used to visualize details in the AL and antennal nerve (AN) during de- and regeneration. Within 24 h post-crush (hpc) the ORN fragments distal to the lesion degenerated. After 48 hpc, regenerating fibers grew through the crush site. In the AL, labeled ORN projections disappeared completely and reappeared after a few days. A weak topographic match between ORN origin on the antenna and the position of innervated glomeruli that was present in untreated controls did not reappear after regeneration. Third, the cell surface marker fasciclin I that is expressed in ORNs was used for quantifying purposes. Immunofluorescence was measured in the AL during de- and regeneration in adults and 5th instar nymphs: after a rapid but transient, decrease, it reappeared. Both processes happen faster in 5th instar nymphs than in adults.
[Mh] Termos MeSH primário: Envelhecimento/fisiologia
Axotomia
Moléculas de Adesão Celular Neuronais/metabolismo
Locusta migratoria/fisiologia
Neurônios Receptores Olfatórios/fisiologia
Regeneração
[Mh] Termos MeSH secundário: Animais
Antenas de Artrópodes/inervação
Antenas de Artrópodes/metabolismo
Encéfalo/metabolismo
Imunofluorescência
Larva/metabolismo
Lasers
Coloração e Rotulagem
Tomografia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Adhesion Molecules, Neuronal); 0 (fasciclin I)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1007/s00441-016-2560-1


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[PMID]:28142212
[Au] Autor:Miljus N; Massih B; Weis MA; Rison JV; Bonnas CB; Sillaber I; Ehrenreich H; Geurten BR; Heinrich R
[Ad] Endereço:Department of Cellular Neurobiology, Institute for Zoology, University of Goettingen, Goettingen, Germany.
[Ti] Título:Neuroprotection and endocytosis: erythropoietin receptors in insect nervous systems.
[So] Source:J Neurochem;141(1):63-74, 2017 Apr.
[Is] ISSN:1471-4159
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Erythropoietin (Epo) plays a dual role as an erythropoiesis-stimulating hormone and a locally produced cytoprotectant in various vertebrate tissues. Splice variants and engineered derivatives of Epo that mediate neuroprotection but do not stimulate erythropoiesis suggest that alternative receptors, different from the 'classical' homodimeric receptor involved in haematopoiesis, mediate neuroprotective Epo functions. Previous studies on grasshoppers demonstrated neuroprotective and neuroregenerative effects of Epo that involved similar transduction pathways as in mammals. To advance the characterization of yet unidentified neuroprotective Epo receptors, we studied the neuroprotective potency of the human non-erythropoietic Epo splice variant EV-3 in primary cultured locust brain neurons. We demonstrate that EV-3, like Epo, protects locust neurons from hypoxia-induced apoptotic death through activation of the Janus kinase/signal transducer and activator of transcription transduction pathway. Using the fluorescent dye FM1-43 to quantify endocytotic activity we show that both Epo and EV-3 increase the number of fluorescently labelled endocytotic vesicles. This reveals that binding of Epo to its neuroprotective receptor induces endocytosis, as it has been described for the mammalian homodimeric Epo-receptor expressed by erythroid progenitors. Reduction in Epo-stimulated endocytotic activity following pre-exposure to EV-3 indicated that both Epo and its splice variant bind to the same receptor on locust neurons. The shared neuroprotective potency of Epo and EV-3 in insect and mammalian neurons, in the absence of erythropoietic effects of EV-3 in mammals, suggests a greater similarity of the unidentified nervous Epo receptors (or receptor complexes) across phyla than between mammalian haematopoietic and neuroprotective receptors. Insects may serve as suitable models to evaluate the specific protective mechanisms mediated by Epo and its variants in non-erythropoietic mammalian tissues.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Endocitose/fisiologia
Neuroproteção/fisiologia
Receptores da Eritropoetina/metabolismo
[Mh] Termos MeSH secundário: Animais
Encéfalo/efeitos dos fármacos
Células CHO
Células Cultivadas
Cricetinae
Cricetulus
Endocitose/efeitos dos fármacos
Eritropoetina/metabolismo
Eritropoetina/farmacologia
Feminino
Seres Humanos
Insetos
Locusta migratoria
Masculino
Neuroproteção/efeitos dos fármacos
Receptores da Eritropoetina/agonistas
Proteínas Recombinantes/metabolismo
Proteínas Recombinantes/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (EPO protein, human); 0 (Receptors, Erythropoietin); 0 (Recombinant Proteins); 11096-26-7 (Erythropoietin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.1111/jnc.13967



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