Base de dados : MEDLINE
Pesquisa : B01.050.500.131.617.678.410 [Categoria DeCS]
Referências encontradas : 1627 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 163 ir para página                         

  1 / 1627 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28746421
[Au] Autor:Sadowska-Bartosz I; Furmaniak P; Bieszczad-Bedrejczuk E; Bartosz G; Glowacki R
[Ad] Endereço:Department of Analytical Biochemistry, Faculty of Biology and Agriculture, University of Rzeszow, Rzeszów, Poland.
[Ti] Título:Developmental changes in the levels and redox potentials of main hemolymph thiols/disulfides in the Jamaican field cricket Gryllus assimilis.
[So] Source:Acta Biochim Pol;64(3):503-506, 2017.
[Is] ISSN:1734-154X
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Main thiols and disulfides were determined in the hemolymph of the Jamaican field cricket Gryllus assimilis at various developmental stages. On the basis of these data, redox potentials of the glutathione, cysteine and homocysteine redox systems were calculated. The concentrations of all thiols studied decreased during development (at a stage of 6 molts) with respect to young crickets, and increased again in adult insects. Redox potentials of the glutathione and cysteine systems increased from values of -131.0±5.6 mV and -86.9±17.1 mV, respectively in young crickets to -58.0±3.6 mV and -36.1±4.2 mV, respectively, at the stage of 6 molts and decreased to values of -110.4±24.8 mV and -66.3±12.2 mV, respectively, in adult insects. Redox potentials of the glutathione and cysteine systems in the hemolymph of young and adult insects were similar to those reported for human plasma.
[Mh] Termos MeSH primário: Dissulfetos/metabolismo
Gryllidae/crescimento & desenvolvimento
Gryllidae/metabolismo
Hemolinfa/metabolismo
Compostos de Sulfidrila/metabolismo
[Mh] Termos MeSH secundário: Animais
Cisteína/metabolismo
Glutationa/metabolismo
Dissulfeto de Glutationa/metabolismo
Homocisteína/metabolismo
Ninfa/crescimento & desenvolvimento
Ninfa/metabolismo
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disulfides); 0 (Sulfhydryl Compounds); 0LVT1QZ0BA (Homocysteine); GAN16C9B8O (Glutathione); K848JZ4886 (Cysteine); ULW86O013H (Glutathione Disulfide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.18388/abp.2017_1510


  2 / 1627 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29258861
[Au] Autor:Carcamo-Noriega EN; Olamendi-Portugal T; Restano-Cassulini R; Rowe A; Uribe-Romero SJ; Becerril B; Possani LD
[Ad] Endereço:Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, UNAM, Av. Universidad 2001, Apartado Postal 510-3, Cuernavaca, Morelos 62210, Mexico. Electronic address: edsoncar@ibt.unam.mx.
[Ti] Título:Intraspecific variation of Centruroides sculpturatus scorpion venom from two regions of Arizona.
[So] Source:Arch Biochem Biophys;638:52-57, 2018 01 15.
[Is] ISSN:1096-0384
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated geographic variability in the venom of Centruroides sculpturatus scorpions from different biotopes. Venom from scorpions collected from two different regions in Arizona; Santa Rita Foothills (SR) and Yarnell (Yar) were analyzed. We found differences between venoms, mainly in the two most abundant peptides; SR (CsEv2e and CsEv1f) and Yar (CsEv2 and CsEv1c) identified as natural variants of CsEv1 and CsEv2. Sequence analyses of these peptides revealed conservative amino acid changes between variants, which may underlie biological activity against arthropods. A third peptide (CsEv6) was highly abundant in the Yar venom compared to the SR venom. CsEv6 is a 67 amino acid peptide with 8 cysteines. CsEv6 did not exhibit toxicity to the three animal models tested. However, both venoms shared similarities in peptides that are predicted to deter predators. For example, both venoms expressed CsEI (lethal to chick) in similar abundance, while CsEd and CsEM1a (toxic to mammals) displayed only moderate variation in their abundance. Electrophysiological evaluation of CsEd and CsEM1a showed that both toxins act on the human sodium-channel subtype 1.6 (hNav 1.6). Complete sequencing revealed that both toxins are structurally similar to beta-toxins isolated from different Centruroides species that also target hNav 1.6.
[Mh] Termos MeSH primário: Proteínas de Artrópodes
Variação Genética
Venenos de Escorpião
Escorpiões
[Mh] Termos MeSH secundário: Animais
Arizona
Proteínas de Artrópodes/química
Proteínas de Artrópodes/genética
Proteínas de Artrópodes/toxicidade
Células CHO
Galinhas
Cricetulus
Gryllidae
Células HEK293
Seres Humanos
Camundongos
Canal de Sódio Disparado por Voltagem NAV1.6/genética
Canal de Sódio Disparado por Voltagem NAV1.6/metabolismo
Venenos de Escorpião/química
Venenos de Escorpião/genética
Venenos de Escorpião/toxicidade
Escorpiões/química
Escorpiões/genética
Análise de Sequência de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (Centruroides toxin); 0 (NAV1.6 Voltage-Gated Sodium Channel); 0 (SCN8A protein, human); 0 (Scorpion Venoms); 141322-33-0 (toxin 2, Centruroides noxius)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE


  3 / 1627 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29187608
[Au] Autor:Chivers BD; Jonsson T; Soulsbury CD; Montealegre-Z F
[Ad] Endereço:School of Life Sciences, University of Lincoln, Lincoln LN6 7DL, UK.
[Ti] Título:Structural biomechanics determine spectral purity of bush-cricket calls.
[So] Source:Biol Lett;13(11), 2017 11.
[Is] ISSN:1744-957X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bush-crickets (Orthoptera: Tettigoniidae) generate sound using tegminal stridulation. Signalling effectiveness is affected by the widely varying acoustic parameters of temporal pattern, frequency and spectral purity (tonality). During stridulation, frequency multiplication occurs as a scraper on one wing scrapes across a file of sclerotized teeth on the other. The frequency with which these tooth-scraper interactions occur, along with radiating wing cell resonant properties, dictates both frequency and tonality in the call. Bush-cricket species produce calls ranging from resonant, tonal calls through to non-resonant, broadband signals. The differences are believed to result from differences in file tooth arrangement and wing radiators, but a systematic test of the structural causes of broadband or tonal calls is lacking. Using phylogenetically controlled structural equation models, we show that parameters of file tooth density and file length are the best-fitting predictors of tonality across 40 bush-cricket species. Features of file morphology constrain the production of spectrally pure signals, but systematic distribution of teeth alone does not explain pure-tone sound production in this family.
[Mh] Termos MeSH primário: Comunicação Animal
Gryllidae/anatomia & histologia
Asas de Animais/anatomia & histologia
[Mh] Termos MeSH secundário: Acústica
Animais
Fenômenos Biomecânicos
Gryllidae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180204
[Lr] Data última revisão:
180204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE


  4 / 1627 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29182676
[Au] Autor:Dutta R; Tregenza T; Balakrishnan R
[Ad] Endereço:Centre for Ecology and Conservation, Department of Biosciences, College of Life and Environmental Sciences, University of Exeter, Penryn Campus, Penryn, United Kingdom.
[Ti] Título:Reproductive isolation in the acoustically divergent groups of tettigoniid, Mecopoda elongata.
[So] Source:PLoS One;12(11):e0188843, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sympatric divergent populations of the same species provide an opportunity to study the evolution and maintenance of reproductive isolation. Male mating calls are important in sexual selection in acoustically communicating species, and they also have the potential to maintain isolation among species or incipient species. We studied divergent south Indian populations of the bush cricket Mecopoda elongata which are extremely difficult to distinguish morphologically, but which exhibit striking divergence in male acoustic signals. We performed phonotactic experiments investigating the relative preference of females of the "Chirper" song type for calls of all 5 of the song types found in the region (in varying degrees of sympatry). We found that Chirper females preferred their own song type and were completely unresponsive to three trilling song types. Chirper females were occasionally attracted to the call type "Double Chirper" (the call most similar to their own type), suggesting call preference alone cannot provide a complete isolating mechanism. To investigate the basis of call preference we investigated the response of chirper females to variation in chirp rate. Chirper females responded most frequently to a mean chirp rate characteristic of their own song type rather than a higher chirp rate which would be more characteristic of the Double-Chirper song type. This suggests females drive stabilising selection on male chirp rate, which may contribute to the maintenance of isolation. Finally, a no-choice mating experiment using Chirper females and Chirper and Double Chirper males revealed a significant preference of Chirper females to mate with their own song type, even without a requirement for phonotaxis. Overall, the strong specificity of Chirper females for their 'own' song type provides evidence for behavioural isolation among divergent sympatric Mecopoda song types being maintained by female preference for both male song type and subsequent mating probability driven by other cues.
[Mh] Termos MeSH primário: Acústica
Gryllidae/fisiologia
Isolamento Reprodutivo
Comportamento Sexual Animal
Vocalização Animal
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188843


  5 / 1627 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28977023
[Au] Autor:de Farias-Martins F; Sperber CF; Albeny-Simões D; Breaux JA; Fianco M; Szinwelski N
[Ad] Endereço:Laboratório de Orthoptera, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil.
[Ti] Título:Forest litter crickets prefer higher substrate moisture for oviposition: Evidence from field and lab experiments.
[So] Source:PLoS One;12(10):e0185800, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:For insects, choosing a favorable oviposition site is a type of parental care, as far as it increases the fitness of its offspring. Niche theory predicts that crickets should show a bell-shaped oviposition response to substrate moisture. However, lab experiments with mole crickets showed a linear oviposition response to substrate moisture. Studies with the house cricket Acheta domesticus also showed a linear juvenile body growth response to water availability, thus adult ovipositing females should respond positively to substrate moisture. We used a field experiment to evaluate the relationship between oviposition preference and substrate moisture in forest litter-dwelling cricket species. We also evaluated oviposition responses to substrate moisture level in Ubiquepuella telytokous, the most abundant litter cricket species in our study area, using a laboratory study. We offered cotton substrate for oviposition which varied in substrate moisture level from zero (i.e., dry) to maximum water absorption capacity. We used two complementary metrics to evaluate oviposition preference: (i) presence or absence of eggs in each sampling unit as binary response variable, and (ii) number of eggs oviposited per sampling unit as count response variable. To test for non-linear responses, we adjusted generalized additive models (GAMM) with mixed effects. We found that both cricket oviposition probability and effort (i.e., number of eggs laid) increased linearly with substrate moisture in the field experiment, and for U. telytokous in the lab experiment. We discarded any non-linear responses. Our results demonstrate the importance of substrate moisture as an ecological niche dimension for litter crickets. This work bolsters knowledge of litter cricket life history association with moisture, and suggests that litter crickets may be particularly threatened by changes in climate that favor habitat drying.
[Mh] Termos MeSH primário: Gryllidae/fisiologia
Oviposição
[Mh] Termos MeSH secundário: Animais
Feminino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185800


  6 / 1627 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28911115
[Au] Autor:Gross L; Vicens Q; Einhorn E; Noireterre A; Schaeffer L; Kuhn L; Imler JL; Eriani G; Meignin C; Martin F
[Ad] Endereço:Université de Strasbourg, CNRS, Architecture et Réactivité de l'ARN, UPR 9002, F-67000 Strasbourg, France.
[Ti] Título:The IRES5'UTR of the dicistrovirus cricket paralysis virus is a type III IRES containing an essential pseudoknot structure.
[So] Source:Nucleic Acids Res;45(15):8993-9004, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cricket paralysis virus (CrPV) is a dicistrovirus. Its positive-sense single-stranded RNA genome contains two internal ribosomal entry sites (IRESs). The 5' untranslated region (5'UTR) IRES5'UTR mediates translation of non-structural proteins encoded by ORF1 whereas the well-known intergenic region (IGR) IRESIGR is required for translation of structural proteins from open reading frame 2 in the late phase of infection. Concerted action of both IRES is essential for host translation shut-off and viral translation. IRESIGR has been extensively studied, in contrast the IRES5'UTR remains largely unexplored. Here, we define the minimal IRES element required for efficient translation initiation in drosophila S2 cell-free extracts. We show that IRES5'UTR promotes direct recruitment of the ribosome on the cognate viral AUG start codon without any scanning step, using a Hepatitis-C virus-related translation initiation mechanism. Mass spectrometry analysis revealed that IRES5'UTR recruits eukaryotic initiation factor 3, confirming that it belongs to type III class of IRES elements. Using Selective 2'-hydroxyl acylation analyzed by primer extension and DMS probing, we established a secondary structure model of 5'UTR and of the minimal IRES5'UTR. The IRES5'UTR contains a pseudoknot structure that is essential for proper folding and ribosome recruitment. Overall, our results pave the way for studies addressing the synergy and interplay between the two IRES from CrPV.
[Mh] Termos MeSH primário: Regiões 5´ não Traduzidas
Dicistroviridae/genética
Sítios Internos de Entrada Ribossomal
Biossíntese de Proteínas
RNA Viral/química
Proteínas Virais/química
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Linhagem Celular
Sistema Livre de Células/metabolismo
Dicistroviridae/crescimento & desenvolvimento
Dicistroviridae/metabolismo
Drosophila melanogaster/virologia
Gryllidae/virologia
Interações Hospedeiro-Patógeno
Conformação de Ácido Nucleico
Fases de Leitura Aberta
RNA Viral/genética
RNA Viral/metabolismo
Ribossomos/genética
Ribossomos/metabolismo
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (Internal Ribosome Entry Sites); 0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx622


  7 / 1627 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28910319
[Au] Autor:Rose J; Rillich J; Stevenson PA
[Ad] Endereço:Institute for Biology, Leipzig University, Talstrasse, Leipzig, Germany.
[Ti] Título:Chronic social defeat induces long-term behavioral depression of aggressive motivation in an invertebrate model system.
[So] Source:PLoS One;12(9):e0184121, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Losing a fight against a conspecific male (social defeat) induces a period of suppressed aggressiveness and general behaviour, often with symptoms common to human psychiatric disorders. Agonistic experience is also discussed as a potential cause of consistent, behavioral differences between individuals (animal "personality"). In non-mammals, however, the impact of single agonistic encounters typically last only hours, but then again studies of repeated intermittent defeat (chronic social defeat) are seldom. We report the effect of chronic social defeat in adult male crickets (Gryllus bimaculatus), for which all known behavioral effects of defeat last only 3 h. Firstly, after 48 h social isolation, crickets that experienced 5 defeats at 24 h intervals against the same, weight-matched opponent exhibited suppressed aggressiveness lasting >24 h, which was still evident when the animals were matched against an unfamiliar opponent at the last trial. Secondly, this longer-term depression of aggression also occurred in 48 h isolated crickets that lost 6 fights at 1 h intervals against unfamiliar opponents at each trial. Thirdly, crickets isolated as larvae until adult maturity (>16 days) were significantly more aggressive, and less variable in their aggressiveness at their very first fight than 48 h isolates, and also significantly more resilient to the effects of chronic social defeat. We conclude that losing an aggressive encounter in crickets has a residual effect, lasting at least 24 h, that accumulates when repeated defeats are experienced, and leads to a prolonged depression of aggressive motivation in subordinates. Furthermore, our data indicate that social interactions between young adults and possibly larvae can have even longer, possibly lifelong influences on subsequent behavior. Social subjugation is thus likely to be a prime determinant of inter-individual behavioral differences in crickets. Our work also opens new avenues for investigating proximate mechanisms underlying depression-like phenomena.
[Mh] Termos MeSH primário: Agressão
Comportamento Animal
Depressão
Gryllidae
Modelos Biológicos
Comportamento Social
[Mh] Termos MeSH secundário: Animais
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184121


  8 / 1627 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28791459
[Au] Autor:Kosumi T; Takeda M
[Ad] Endereço:Laboratory of Molecular Insect Science, Graduate School of Agricultural Science, Kobe University, Kobe, 657-8501, Japan.
[Ti] Título:Three-year lifecycle, large body, and very high threshold temperature in the cricket Gryllus argenteus for special adaptation to desiccation cycle in Malawi.
[So] Source:Naturwissenschaften;104(9-10):70, 2017 Aug 08.
[Is] ISSN:1432-1904
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In temperate climates, the initiation and termination of diapause synchronize the stress-tolerant stage with the stressful season and reproduction with the non-stressful season in many insects. Synchronization is often regulated by photoperiodism.Voltinism and the ultimate size of adults are also important determinants for their lifecycle, and different diapause stages and voltinism patterns are known in crickets.Here, we investigated the life history of the African cricket Gryllus argenteus from Malawi, which is a typical arid tropical highland. The climate is characterized by alternating arid and wet seasons, each of which lasts for half a year, and where the available heat mass is much less than lowlands at the same latitude. We first measured the nymphal duration at each rearing temperature and calculated the lower developmental threshold (t ) to be 20.19 °C based on Ikemoto and Takai (2000) and 19.38 °C based on a conventional line-fitting method. These values are very high relative to many other insects. The local temperature in winter does not fall below 15 °C, but this is much higher than the lethal limit. This suggested that critical stress in this locality was not coldness but low precipitation in winter. We estimated, based both on local temperature change and the Ikemoto and Takai's t , that G. argenteus required 3 years to complete its lifecycle unlike wet lowland species, where univoltinism or multi-voltinism are commonplace. Photoperiodism was observed in this species, but due to a lag between annual cycles in photoperiod, temperature, and humidity, photoperiodism alone cannot atune their lifecycle with local conditions.Synchronization in this species was achieved by three different adaptations: photoperiodism, high t , and large body size, which give it a long lifecycle. Although the species cannot achieve a univoltine lifecycle because of its high t value, it can escape from dry season by entering diapause at moderate temperatures, probably thereby achieving adaptive synchrony of lifecycle with both favorable and unfavorable seasons. A comparison between a conventional photothermogram and a newly formulated photohydrogram or photohygrogram demonstrates that even though sufficient heat is available, scarcity of water and thus scarcity of foliage should force the cricket to maintain diapause at intermediate temperature. The results suggested that high t , large body size, and multi-ennial lifecycle mutually affect each other and formulate a unique adaptation under such an extreme environment.
[Mh] Termos MeSH primário: Gryllidae
[Mh] Termos MeSH secundário: Aclimatação
Animais
Dessecação
Malaui
Fotoperíodo
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1007/s00114-017-1488-y


  9 / 1627 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28768714
[Au] Autor:Bugaud O; Barbier N; Chommy H; Fiszman N; Le Gall A; Dulin D; Saguy M; Westbrook N; Perronet K; Namy O
[Ad] Endereço:Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91190 Gif sur Yvette, France.
[Ti] Título:Kinetics of CrPV and HCV IRES-mediated eukaryotic translation using single-molecule fluorescence microscopy.
[So] Source:RNA;23(11):1626-1635, 2017 Nov.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Protein synthesis is a complex multistep process involving many factors that need to interact in a coordinated manner to properly translate the messenger RNA. As translating ribosomes cannot be synchronized over many elongation cycles, single-molecule studies have been introduced to bring a deeper understanding of prokaryotic translation dynamics. Extending this approach to eukaryotic translation is very appealing, but initiation and specific labeling of the ribosomes are much more complicated. Here, we use a noncanonical translation initiation based on internal ribosome entry sites (IRES), and we monitor the passage of individual, unmodified mammalian ribosomes at specific fluorescent milestones along mRNA. We explore initiation by two types of IRES, the intergenic IRES of cricket paralysis virus (CrPV) and the hepatitis C (HCV) IRES, and show that they both strongly limit the rate of the first elongation steps compared to the following ones, suggesting that those first elongation cycles do not correspond to a canonical elongation. This new system opens the possibility of studying both IRES-mediated initiation and elongation kinetics of eukaryotic translation and will undoubtedly be a valuable tool to investigate the role of translation machinery modifications in human diseases.
[Mh] Termos MeSH primário: Dicistroviridae/genética
Dicistroviridae/metabolismo
Hepacivirus/genética
Hepacivirus/metabolismo
Sítios Internos de Entrada Ribossomal
Biossíntese de Proteínas
[Mh] Termos MeSH secundário: Animais
Gryllidae/virologia
Seres Humanos
Técnicas In Vitro
Cinética
Microscopia de Fluorescência
Modelos Biológicos
Elongação Traducional da Cadeia Peptídica
RNA Mensageiro/genética
RNA Viral/genética
Coelhos
Ribossomos/metabolismo
Imagem Individual de Molécula
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Internal Ribosome Entry Sites); 0 (RNA, Messenger); 0 (RNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1261/rna.061523.117


  10 / 1627 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28664787
[Au] Autor:Fukumura K; Nagata S
[Ad] Endereço:a Department of Integrated Biosciences , Graduate School of Frontier Sciences, The University of Tokyo , Chiba , Japan.
[Ti] Título:Behavioral tracing demonstrates dietary nutrient discrimination in two-spotted crickets Gryllus bimaculatus.
[So] Source:Biosci Biotechnol Biochem;81(10):1990-1993, 2017 Oct.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Animals select appropriate diets to meet their nutritional requirements. Here, we demonstrate the availability for analysis of feeding preference using an orthopteran, the two-spotted cricket Gryllus bimaculatus. A time-course study of these insects, involving continuous recording and tracing behavior for 9 h, allowed us to monitor discrimination of diet that contained various nutrients.
[Mh] Termos MeSH primário: Dieta
Comportamento Alimentar/efeitos dos fármacos
Gryllidae/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Ácidos Graxos/farmacologia
Gryllidae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1343119



página 1 de 163 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde