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Pesquisa : B01.050.500.131.617.720.500.500.375.307 [Categoria DeCS]
Referências encontradas : 362 [refinar]
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  1 / 362 MEDLINE  
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[PMID]:29280364
[Au] Autor:Liu GC; Dong ZW; He JW; Zhao RP; Wang W; Li XY
[Ad] Endereço:State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China.
[Ti] Título:Genome size of 14 species of fireflies (Insecta, Coleoptera, Lampyridae).
[So] Source:Zool Res;38(6):449-458, 2017 Nov 18.
[Is] ISSN:2095-8137
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Eukaryotic genome size data are important both as the basis for comparative research into genome evolution and as estimators of the cost and difficulty of genome sequencing programs for non-model organisms. In this study, the genome size of 14 species of fireflies (Lampyridae) (two genera in Lampyrinae, three genera in Luciolinae, and one genus in subfamily ) were estimated by propidium iodide (PI)-based flow cytometry. The haploid genome sizes of Lampyridae ranged from 0. 42 to 1. 31 pg, a 3. 1-fold span. Genome sizes of the fireflies varied within the tested subfamilies and genera. and species had large and small genome sizes, respectively. No correlation was found between genome size and morphological traits such as body length, body width, eye width, and antennal length. Our data provide additional information on genome size estimation of the firefly family Lampyridae. Furthermore, this study will help clarify the cost and difficulty of genome sequencing programs for non-model organisms and will help promote studies on firefly genome evolution.
[Mh] Termos MeSH primário: Vaga-Lumes/genética
Tamanho do Genoma
Genoma de Inseto
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.24272/j.issn.2095-8137.2017.078


  2 / 362 MEDLINE  
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[PMID]:28687486
[Au] Autor:Branchini BR; Southworth TL; Fontaine DM; Kohrt D; Welcome FS; Florentine CM; Henricks ER; DeBartolo DB; Michelini E; Cevenini L; Roda A; Grossel MJ
[Ad] Endereço:Department of Chemistry, Connecticut College, New London, CT 06320, USA. Electronic address: brbra@conncoll.edu.
[Ti] Título:Red-emitting chimeric firefly luciferase for in vivo imaging in low ATP cellular environments.
[So] Source:Anal Biochem;534:36-39, 2017 Oct 01.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Beetle luciferases have been adapted for live cell imaging where bioluminescence is dependent on the cellular availability of ATP, O , and added luciferin. Previous Photinus pyralis red-emitting variants with high K values for ATP have performed disappointingly in live cells despite having much higher relative specific activities than enzymes like Click Beetle Red (CBR). We engineered a luciferase variant PLR3 having a K value for ATP similar to CBR and ∼2.6-fold higher specific activity. The red-emitting PLR3 was ∼2.5-fold brighter than CBR in living HEK293T and HeLa cells, an improvement consistent with the importance of the K value in low ATP environments.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/análise
Luciferases de Vaga-Lume/química
Medições Luminescentes
[Mh] Termos MeSH secundário: Animais
Vaga-Lumes
Células HEK293
Células HeLa
Seres Humanos
Luciferases de Vaga-Lume/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
8L70Q75FXE (Adenosine Triphosphate); EC 1.13.12.7 (Luciferases, Firefly)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170709
[St] Status:MEDLINE


  3 / 362 MEDLINE  
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[PMID]:28672033
[Au] Autor:Jazayeri FS; Amininasab M; Hosseinkhani S
[Ad] Endereço:Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, IRAN.
[Ti] Título:Structural and dynamical insight into thermally induced functional inactivation of firefly luciferase.
[So] Source:PLoS One;12(7):e0180667, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Luciferase is the key component of light production in bioluminescence process. Extensive and advantageous application of this enzyme in biotechnology is restricted due to its low thermal stability. Here we report the effect of heating up above Tm on the structure and dynamical properties of luciferase enzyme compared to temperature at 298 K. In this way we demonstrate that the number of hydrogen bonds between N- and C-domain is increased for the free enzyme at 325 K. Increased inter domain hydrogen bonds by three at 325 K suggests that inter domain contact is strengthened. The appearance of simultaneous strong salt bridge and hydrogen bond between K529 and D422 and increased existence probability between R533 and E389 could mechanistically explain stronger contact between N- and C-domain. Mutagenesis studies demonstrated the importance of K529 and D422 experimentally. Also the significant reduction in SASA for experimentally important residues K529, D422 and T343 which are involved in active site region was observed. Principle component analysis (PCA) in our study shows that the dynamical behavior of the enzyme is changed upon heating up which mainly originated from the change of motion modes and associated extent of those motions with respect to 298 K. These findings could explain why heating up of the enzyme or thermal fluctuation of protein conformation reduces luciferase activity in course of time as a possible mechanism of thermal functional inactivation. According to these results we proposed two strategies to improve thermal stability of functional luciferase.
[Mh] Termos MeSH primário: Luciferases de Vaga-Lume/metabolismo
[Mh] Termos MeSH secundário: Animais
Análise por Conglomerados
Vaga-Lumes
Ligações de Hidrogênio
Luciferases de Vaga-Lume/química
Análise de Componente Principal
Estrutura Secundária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.13.12.7 (Luciferases, Firefly)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180667


  4 / 362 MEDLINE  
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[PMID]:28610049
[Au] Autor:Novák M
[Ad] Endereço:Department of Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, Praha 6 - Suchdol, CZ-165 21, Czech Republic.. martas.novak@centrum.cz.
[Ti] Título:Redescription of immature stages of central European fireflies, Part 1: Lampyris noctiluca (Linnaeus, 1758) larva, pupa and notes on its biology (Coleoptera: Lampyridae: Lampyrinae).
[So] Source:Zootaxa;4247(4):429-444, 2017 Mar 28.
[Is] ISSN:1175-5334
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:The mature larva of Lampyris noctiluca (Linnaeus, 1758) is redescribed and illustrated in detail, including scanning electron microscope images. Male and female pupae are briefly described, including notes on behaviour as well as light production of the immature stages. Observed structures, life cycle and behaviour of larvae and pupae are discussed.
[Mh] Termos MeSH primário: Vaga-Lumes
[Mh] Termos MeSH secundário: Animais
Europa (Continente)
Feminino
Larva
Masculino
Pupa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.11646/zootaxa.4247.4.5


  5 / 362 MEDLINE  
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[PMID]:28541478
[Au] Autor:Lower SS; Johnston JS; Stanger-Hall KF; Hjelmen CE; Hanrahan SJ; Korunes K; Hall D
[Ad] Endereço:Department of Genetics, University of Georgia.
[Ti] Título:Genome Size in North American Fireflies: Substantial Variation Likely Driven by Neutral Processes.
[So] Source:Genome Biol Evol;9(6):1499-1512, 2017 Jun 01.
[Is] ISSN:1759-6653
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Eukaryotic genomes show tremendous size variation across taxa. Proximate explanations for genome size variation include differences in ploidy and amounts of noncoding DNA, especially repetitive DNA. Ultimate explanations include selection on physiological correlates of genome size such as cell size, which in turn influence body size, resulting in the often-observed correlation between body size and genome size. In this study, we examined body size and repetitive DNA elements in relationship to the evolution of genome size in North American representatives of a single beetle family, the Lampyridae (fireflies). The 23 species considered represent an excellent study system because of the greater than 5-fold range of genome sizes, documented here using flow cytometry, and the 3-fold range in body size, measured using pronotum width. We also identified common genomic repetitive elements using low-coverage sequencing. We found a positive relationship between genome size and repetitive DNA, particularly retrotransposons. Both genome size and these elements were evolving as expected given phylogenetic relatedness. We also tested whether genome size varied with body size and found no relationship. Together, our results suggest that genome size is evolving neutrally in fireflies.
[Mh] Termos MeSH primário: Vaga-Lumes/genética
Tamanho do Genoma
Genoma de Inseto/genética
[Mh] Termos MeSH secundário: Animais
Tamanho Corporal
Evolução Molecular
Citometria de Fluxo
Variação Genética
América do Norte
Filogenia
Retroelementos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Retroelements)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1093/gbe/evx097


  6 / 362 MEDLINE  
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[PMID]:28431298
[Au] Autor:Rabha MM; Sharma U; Goswami A; Gohain Barua A
[Ad] Endereço:Department of Physics, Gauhati University, Guwahati 781014, India.
[Ti] Título:Bioluminescence emissions of female fireflies of the species Luciola praeusta.
[So] Source:J Photochem Photobiol B;170:134-139, 2017 May.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Numerous investigations have been carried out on bioluminescence emissions from male fireflies. However, very few observations have been made on the emitted light from female specimens. Even in those, apart from observing responses from females to courtship flashes from conspecific males, detailed studies have not been performed. Here we present a first report on the light of female fireflies of the Indian species Luciola praeusta Kiesenwetter 1874 (Coleoptera:Lampyridae:Luciolinae). In the steady-state emission spectrum over the temperature range of 20-40°C, the peak wavelength is the same as, while the full width at half maximum is larger than, that of a male specimen of this species. Increase in temperature up to 45°C brings out a change in both the peak and FWHM values, shifting towards red. In the time-resolved measurement, duration of a flash, which is noticeably larger than that of a male, is found to decrease exponentially with temperature at 20-40°C. Further increase in the temperature produces a minimum flash duration at 41.5°C, and beyond this causes a considerable increase in duration for small increase in temperature. Additionally, lowering the temperature below 20°C makes a single flash appear as a combination of two or three flashes.
[Mh] Termos MeSH primário: Vaga-Lumes/fisiologia
Medições Luminescentes
[Mh] Termos MeSH secundário: Animais
Feminino
Vaga-Lumes/química
Masculino
Temperatura Ambiente
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE


  7 / 362 MEDLINE  
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[PMID]:28336069
[Au] Autor:Branchini BR; Southworth TL
[Ad] Endereço:Connecticut College, New London, CT, United States. Electronic address: brbra@conncoll.edu.
[Ti] Título:A Highly Sensitive Biosensor for ATP Using a Chimeric Firefly Luciferase.
[So] Source:Methods Enzymol;589:351-364, 2017.
[Is] ISSN:1557-7988
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Firefly luciferases, which emit visible light in a highly specific ATP-dependent process, have been adapted for a variety of applications based on the detection of the enzymes or using the proteins to measure ATP levels. Based on studies of chimeric luciferases, we engineered a novel luciferase called PLG2 that has enhanced specific activity, and thermal and pH stability compared to the commonly used Photinus pyralis luciferase. We present here protocols for preparing a single assay mixture containing PLG2 that can be used to readily detect femtomole levels of ATP. Our methodology can be used with a variety of samples, including human and bacterial cells, where measurements of ATP can be used as a biosensor for the detection of viable cells.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/análise
Técnicas Biossensoriais/métodos
Luciferases de Vaga-Lume/análise
Substâncias Luminescentes/análise
Medições Luminescentes/métodos
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Animais
Vaga-Lumes/enzimologia
Vaga-Lumes/genética
Células HEK293
Seres Humanos
Luciferases de Vaga-Lume/genética
Luciferases de Vaga-Lume/metabolismo
Substâncias Luminescentes/metabolismo
Engenharia de Proteínas/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Luminescent Agents); 8L70Q75FXE (Adenosine Triphosphate); EC 1.13.12.7 (Luciferases, Firefly)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE


  8 / 362 MEDLINE  
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[PMID]:28187659
[Au] Autor:Silveira LF; Mermudes JR
[Ad] Endereço:Programa de Pós-Graduação em Ecologia/UFRJ, Laboratório de Ecologia de Insetos, Departamento de Ecologia, Instituto de Biologia, Universidade Federal do Rio de Janeiro, A0-113, Bloco A, Av. Carlos Chagas Filho, 373, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ, Brazil Laboratório de Entomologia, Departamento de Zoologia, Instituto de Biologia, Universidade Federal do Rio de Janeiro, A1-107, Bloco A, Av. Carlos Chagas Filho, 373, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ, Brazil. user@example.com.
[Ti] Título:A new tropical montane firefly genus and species, active during winter and endemic to the southeastern Atlantic Rainforest (Coleoptera: Lampyridae).
[So] Source:Zootaxa;4221(2):zootaxa.4221.2.4, 2017 Jan 17.
[Is] ISSN:1175-5334
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Here we describe Araucariocladus hiems gen. et sp. nov. (Lampyridae: Amydetinae), a firefly species endemic to high montane forests, and occurring during June, a relatively cool and dry month in the Southeastern Atlantic Rainforest of Brazil. We tentatively place it in Psilocladina McDermott, and discuss the limitations of its classification. We also provide illustrations of key structural features of the new taxa and discuss its affinities.
[Mh] Termos MeSH primário: Vaga-Lumes
[Mh] Termos MeSH secundário: Animais
Brasil
Floresta Úmida
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170212
[St] Status:MEDLINE
[do] DOI:10.11646/zootaxa.4221.2.4


  9 / 362 MEDLINE  
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[PMID]:27998815
[Au] Autor:Martin GJ; Branham MA; Whiting MF; Bybee SM
[Ad] Endereço:Department of Biology, 4102 LSB, Brigham Young University, Provo, UT 84602, USA. Electronic address: gavin.jon.martin@gmail.com.
[Ti] Título:Total evidence phylogeny and the evolution of adult bioluminescence in fireflies (Coleoptera: Lampyridae).
[So] Source:Mol Phylogenet Evol;107:564-575, 2017 Feb.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fireflies are some of the most captivating organisms on the planet. They have a rich history as subjects of scientific study, especially in relation to their bioluminescent behavior. Yet, the phylogenetic relationships of fireflies are still poorly understood. Here, we present the first total evidence approach to reconstruct lampyrid phylogeny using both a molecular matrix from six loci and an extensive morphological matrix. Using this phylogeny we test the hypothesis that adult bioluminescence evolved after the origin of the firefly clade. The ancestral state of adult bioluminescence is recovered as non-bioluminescent with one to six gains and five to ten subsequent losses. The monophyly of the family, as well as the subfamilies is also tested. Ototretinae, Cyphonocerinae, Luciolinae (incl. Pristolycus), Amydetinae, "cheguevarinae" sensu Jeng 2008, and Photurinae are highly supported as monophyletic. With the exception of four taxa, Lampyrinae is also recovered as monophyletic with high support. Based on phylogenetic and morphological data Lamprohiza, Phausis, and Lamprigera are transferred to Lampyridae incertae sedis.
[Mh] Termos MeSH primário: Vaga-Lumes/classificação
Luminescência
Filogenia
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
Modelos Teóricos
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE


  10 / 362 MEDLINE  
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[PMID]:27956170
[Au] Autor:Smirnov NA; Akopov SB; Didych DA; Nikolaev LG
[Ad] Endereço:Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
[Ti] Título:In trans promoter activation by enhancers in transient transfection.
[So] Source:Gene;603:15-20, 2017 Mar 01.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Earlier, it was reported that the strong cytomegalovirus enhancer can activate the cytomegalovirus promoter in trans, i.e. as a separate plasmid co-transfected with a promoter-reporter gene construct. Here we demonstrate that the ability of enhancers to activate promoters in trans in transient transfection experiments is a property of not only viral regulatory elements but also of various genomic enhancers and promoters. Enhancer-promoter activation in trans is promoter- and cell type-specific, and accompanied by physical interaction between promoter and enhancer as revealed by chromosome conformation capture assays. Thus, promoter activation in transient co-transfection of promoters and enhancers shares a number of important traits with long-distance promoter activation by enhancers in living cells and may therefore serve as a model of this fundamental cellular process.
[Mh] Termos MeSH primário: Elementos Facilitadores Genéticos
Plasmídeos/metabolismo
Regiões Promotoras Genéticas
Transativadores/genética
Ativação Transcricional
Transfecção/métodos
[Mh] Termos MeSH secundário: Animais
Citomegalovirus/genética
Citomegalovirus/metabolismo
Vaga-Lumes/enzimologia
Vaga-Lumes/genética
Genes Reporter
Células HeLa
Células Hep G2
Seres Humanos
Luciferases/genética
Luciferases/metabolismo
Especificidade de Órgãos
Plasmídeos/química
Renilla/enzimologia
Renilla/genética
Transativadores/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Trans-Activators); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170202
[Lr] Data última revisão:
170202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE



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