Base de dados : MEDLINE
Pesquisa : B01.050.500.131.617.720.500.500.375.615 [Categoria DeCS]
Referências encontradas : 806 [refinar]
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[PMID]:29401571
[Au] Autor:Kryukov VY; Rotskaya UN; Yaroslavtseva ON; Elisaphenko EA; Duisembekov BA; Glupov VV
[Ti] Título:[Phenotypic and genetic changes of entomopathogenic ascomycete Beauveria Bassiana under passaging through various hosts].
[So] Source:Parazitologiia;51(1):3-14, 2017 Jan-Feb.
[Is] ISSN:0031-1847
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Phenotypic and genetic estimations of entomopathogenic ascomycete B.bassiana (strain Sar-31) after 6-passaging through four hosts were shown. Increasing of virulence, changes in morpho-cultural characteristics and variations in Inter Simple Sequence Repeats (ISSR) assay between initial and reisolated cultures were registered. Six passages of entomopathogenic ascomycete Beauveria bassiana (strain Sar-31) through four hosts (Galleria mellonella, Tenebrio molitor, Leptinotarsa decemlineata, Locusta migratoria) and following estimation of phenotypic and genetic differences of the initial strain and reisolated cultures were conducted. The passaging of strain through certain host led to increasing of virulence for both this host and other test-insects. Unidirectional changes of morpho-cultural characteristics: colonies pigmentation and relief strengthening, increasing of conidia production and lipolytic activity were registered in all passaged cultures. Genetic analysis with 6 ISSR markers revealed variations between initial and reisolated cultures in 3 markers. Taken together, the results of this study help us understand potential ways of fungi strains changes during epizootic process and possibilities of ISSR assay applying for investigation of pathogen transmission.
[Mh] Termos MeSH primário: Beauveria/genética
Beauveria/patogenicidade
Proteínas Fúngicas/genética
Especificidade de Hospedeiro
Esporos Fúngicos/genética
Esporos Fúngicos/patogenicidade
[Mh] Termos MeSH secundário: Animais
Beauveria/enzimologia
Beauveria/crescimento & desenvolvimento
Coleópteros/microbiologia
Proteínas Fúngicas/metabolismo
Marcadores Genéticos
Genótipo
Lipólise
Locusta migratoria/microbiologia
Repetições de Microssatélites
Mariposas/microbiologia
Fenótipo
Pigmentação/genética
Inoculações Seriadas
Esporos Fúngicos/enzimologia
Esporos Fúngicos/crescimento & desenvolvimento
Tenebrio/microbiologia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Genetic Markers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180206
[St] Status:MEDLINE


  2 / 806 MEDLINE  
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[PMID]:29244828
[Au] Autor:Janssen RH; Lakemond CMM; Fogliano V; Renzone G; Scaloni A; Vincken JP
[Ad] Endereço:Food Quality and Design, Wageningen University, Wageningen, The Netherlands.
[Ti] Título:Involvement of phenoloxidase in browning during grinding of Tenebrio molitor larvae.
[So] Source:PLoS One;12(12):e0189685, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Insects are investigated as alternative protein source to meet the increasing demand for proteins in the future. Enzymatic browning occurring during grinding of insect and subsequent extraction of proteins can influence the proteins' properties, but it is unclear which enzymes are responsible for this phenomenon. This study was performed on larvae of three commonly used insect species, namely Tenebrio molitor, Alphitobius diaperinus and Hermetia illucens. Oxygen consumption measurements on protein extracts showed activity on L-tyrosine, L-3,4-di-hydroxy-phenylalanine (L-DOPA) and L-dopamine, indicating phenoloxidase as a key player in browning. Furthermore, no reaction on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) was observed, ruling out an important contribution of laccase to browning. The browning reaction was most prominent at pH 6 for T. molitor and A. diaperinus, and 7 for H. illucens. As the enzyme activity of H. illucens was the lowest with the darkest color formation, this was likely caused by another factor. The activity of phenoloxidase was confirmed for T. molitor and A. diaperinus by activity measurements after fractionation by anion-exchange chromatography. Color measurements showed the presence of activity on both L-DOPA and L-tyrosine in the same fractions. Both substrates were converted into dopachrome after incubation with enzyme-enriched fractions. No DOPA-decarboxylase, tyrosine hydroxylase and peroxidase activities were observed. By using native PAGE with L-DOPA as staining-solution, active T. molitor protein bands were resolved and characterized, identifying a tyrosinase/phenoloxidase as the active enzyme species. All together, these data confirmed that tyrosinase is an important enzyme in causing enzymatic browning in T. molitor and likely in A. diaperinus.
[Mh] Termos MeSH primário: Proteínas de Insetos/química
Reação de Maillard
Monofenol Mono-Oxigenase/química
Consumo de Oxigênio/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Coleópteros/química
Coleópteros/genética
Dípteros/química
Dípteros/genética
Dopamina/química
Dopamina/genética
Proteínas de Insetos/genética
Larva/química
Larva/genética
Levodopa/química
Levodopa/genética
Monofenol Mono-Oxigenase/genética
Homologia de Sequência de Aminoácidos
Tenebrio/química
Tenebrio/genética
Tirosina/química
Tirosina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 42HK56048U (Tyrosine); 46627O600J (Levodopa); EC 1.14.18.1 (Monophenol Monooxygenase); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189685


  3 / 806 MEDLINE  
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[PMID]:28743065
[Au] Autor:Yin J; Gao Y; Zhu F; Hao W; Xu Q; Wang H; Guo B
[Ad] Endereço:Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
[Ti] Título:Enantiomerization and stereoselectivity in bioaccumulation of furalaxyl in Tenebrio molitor larvae.
[So] Source:Ecotoxicol Environ Saf;145:244-249, 2017 Nov.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Furalaxyl is a chiral pesticide and widely used in modern agriculture as racemate mixture. The enantiomerization and enantioselecive bioaccumulation by a single dose of furalaxyl to Tenebrio molitor larvae under laboratory conditions were studied using a high-performance liquid chromatography tandem mass spectroscopy method based on a ChiralPAK IC column. Our results showed that a significant enantiomerization (interconversion between R-enantiomer and S-enantiomer) was observed in Tenebrio molitor larvae under R- or S-furalaxyl exposure. Though the two furalaxyl enantiomers exhibited low-capacity of bioaccumulation in Tenebrio molitor larvae, bioaccumulation of rac-furalaxyl was enantioselective with a preferential accumulation of S-furalaxyl at 10mg/kg dosage exposure. In addition, enantiomerization and enantioselective degradation of the two enantiomers was not observed in wheat bran. These results showed that enantioselectivtiy of furalaxyl enantiomers was an important process combined with degradation, metabolism and enatiomerization in organisms.
[Mh] Termos MeSH primário: Poluentes Ambientais/metabolismo
Fungicidas Industriais/metabolismo
Furanos/metabolismo
Tenebrio/metabolismo
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão
Poluentes Ambientais/química
Fungicidas Industriais/química
Furanos/química
Larva/metabolismo
Estereoisomerismo
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Environmental Pollutants); 0 (Fungicides, Industrial); 0 (Furans); 0 (furalaxyl)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171207
[Lr] Data última revisão:
171207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  4 / 806 MEDLINE  
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[PMID]:28867469
[Au] Autor:Kim SG; Jo YH; Seong JH; Park KB; Noh MY; Cho JH; Ko HJ; Kim CE; Tindwa H; Patnaik BB; Bang IS; Lee YS; Han YS
[Ad] Endereço:Division of Plant Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 61186, Republic of Korea.
[Ti] Título:TmSR-C, scavenger receptor class C, plays a pivotal role in antifungal and antibacterial immunity in the coleopteran insect Tenebrio molitor.
[So] Source:Insect Biochem Mol Biol;89:31-42, 2017 Oct.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Scavenger receptors (SRs) constitute a family of membrane-bound receptors that bind to multiple ligands. The SR family of proteins is involved in removing cellular debris, oxidized low-density lipoproteins, and pathogens. Specifically, class C scavenger receptors (SR-C) have also been reported to be involved in phagocytosis of gram-positive and -negative bacteria in Drosophila and viruses in shrimp. However, reports are unavailable regarding the role of SR-C in antifungal immune mechanisms in insects. In this study, a full-length Tenebrio molitor SR-C (TmSR-C) sequence was obtained by 5'- and 3'-Rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). The TmSR-C full-length cDNA comprised 1671 bp with 5'- and 3'-untranslated regions of 23- and 107-bp, respectively. TmSR-C encodes a putative protein of 556 amino acid residues that is constitutively expressed in all tissues of late instar larvae and 2-day-old adults, with the highest transcript levels observed in hemocytes of larvae and adults. TmSR-C mRNA showed a 2.5-fold and 3-fold increase at 24 and 6 h after infection with Candida albicans and ß-glucan, respectively. Immunoassay with TmSR-C polyclonal antibody showed induction of the putative protein in the cytosols of hemocytes at 3 h after inoculation of C. albicans. RNA interference (RNAi)-based gene silencing and phagocytosis assays were used to understand the role of TmSR-C in antifungal immunity. Silencing of TmSR-C transcripts reduced the survivability of late instar larvae at 2 days post-inoculation of C. albicans, Escherichia coli, or Staphylococcus aureus. Furthermore, in TmSR-C-silenced larvae, there was a decline in the rate of microorganism phagocytosis. Taken together, results of this study suggest that TmSR-C plays a pivotal role in phagocytosing not only fungi but also gram-negative and -positive bacteria in T. molitor.
[Mh] Termos MeSH primário: Fagocitose
Receptores Depuradores Classe C/fisiologia
Tenebrio/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Candida albicans
Escherichia coli
Expressão Gênica
Hemócitos/metabolismo
Interferência de RNA
Análise de Sequência de DNA
Staphylococcus aureus
Tenebrio/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Scavenger Receptors, Class C)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE


  5 / 806 MEDLINE  
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[PMID]:28838758
[Au] Autor:Damasceno TF; Dias RO; de Oliveira JR; Salinas RK; Juliano MA; Ferreira C; Terra WR
[Ad] Endereço:Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Avenida Professor Lineu Prestes, 748, São Paulo 05508-000, Brazil.
[Ti] Título:Active subsite properties, subsite residues and targeting to lysosomes or midgut lumen of cathepsins L from the beetle Tenebrio molitor.
[So] Source:Insect Biochem Mol Biol;89:17-30, 2017 Oct.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cathepsins L are the major digestive peptidases in the beetle Tenebrio molitor. Two digestive cathepsins L (TmCAL2 and TmCAL3) from it had their 3D structures solved. The aim of this paper was to study in details TmCAL3 specificity and properties and relate them to its 3D structure. Recombinant TmCAL3 was assayed with 64 oligopeptides with different amino acid replacements in positions P2, P1, P1' and P2'. Results showed that TmCAL3 S2 specificity differs from the human enzyme and that its specificities also explain why on autoactivation two propeptide residues remain in the enzyme. Data on free energy of binding and of activation showed that S1 and S2' are mainly involved in substrate binding, S1' acts in substrate binding and catalysis, whereas S2 is implied mainly in catalysis. Enzyme subsite residues were identified by docking with the same oligopeptide used for kinetics. The subsite hydrophobicities were calculated from the efficiency of hydrolysis of different amino acid replacements in the peptide and from docking data. The results were closer for S1 and S2' than for S1' and S2, indicating that the residue subsites that were more involved in transition state binding are different from those binding the substrate seen in docking. Besides TmCAL1-3, there are nine other cathepsins L, most of them more expressed at midgut. They are supposed to be directed to lysosomes by a Drosophila-like Lerp receptor and/or motifs in their prodomains. The mannose 6-phosphate lysosomal sorting machinery is absent from T. molitor transcriptome. Cathepsin L direction to midgut contents seems to depend on overexpression.
[Mh] Termos MeSH primário: Catepsina L/metabolismo
Tenebrio/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Catálise
Catepsina L/química
Trato Gastrointestinal/enzimologia
Interações Hidrofóbicas e Hidrofílicas
Proteínas de Insetos/química
Proteínas de Insetos/metabolismo
Lisossomos/enzimologia
Simulação de Acoplamento Molecular
Sinais Direcionadores de Proteínas
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Protein Sorting Signals); EC 3.4.22.15 (Cathepsin L)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE


  6 / 806 MEDLINE  
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[PMID]:28699822
[Au] Autor:Przyklenk M; Vemmer M; Hanitzsch M; Patel A
[Ad] Endereço:a Faculty of Engineering and Mathematics, WG Fermentation and Formulation of Biologicals and Chemicals , University of Applied Sciences , Bielefeld , Germany.
[Ti] Título:A bioencapsulation and drying method increases shelf life and efficacy of Metarhizium brunneum conidia.
[So] Source:J Microencapsul;34(5):498-512, 2017 Aug.
[Is] ISSN:1464-5246
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study reports the development of encapsulated and dried entomopathogenic fungus Metarhiuzm brunneum with reduced conidia content, increased conidiation, a high drying survival and enhanced shelf life. Dried beads prepared with the fillers corn starch, potato starch, carboxymethylcellulose or autoclaved baker's yeast, showed enhanced survival with increasing filler content. The maximum survival of 82% was found for beads with 20% corn starch at <0.1 water activity. While increasing starch content inhibits the conidiation, autoclaved baker's yeast and a combination with starch enhanced the conidiation to 1.0 × 10 conidia/bead. Beads with conidia content reduced to 0.01% multiplied conidia in a "microfermentation" by the factor 1000. A bioassay confirmed that conidia formed from rehydrated beads were virulent against Tenebrior molitor larvae. After six months of storage, encapsulated conidia showed improved shelf life compared to non-formulated conidia. This "microfermenter" will pave the way for encapsulated fungi to be used as cost-effective biocontrol agents.
[Mh] Termos MeSH primário: Dessecação
Metarhizium/fisiologia
Controle Biológico de Vetores
Esporos Fúngicos/fisiologia
[Mh] Termos MeSH secundário: Animais
Larva
Tenebrio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1080/02652048.2017.1354941


  7 / 806 MEDLINE  
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[PMID]:28660745
[Au] Autor:Tereshchenkova VF; Goptar IA; Zhuzhikov DP; Belozersky MA; Dunaevsky YE; Oppert B; Filippova IY; Elpidina EN
[Ad] Endereço:Chemical Faculty, Lomonosov Moscow State University, Moscow, Russia.
[Ti] Título:Prolidase is a critical enzyme for complete gliadin digestion in Tenebrio molitor larvae.
[So] Source:Arch Insect Biochem Physiol;95(4), 2017 Aug.
[Is] ISSN:1520-6327
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Prolidase is a proline-specific metallopeptidase that cleaves imidodipeptides with C-terminal Pro residue. Prolidase was purified and characterized from the Tenebrio molitor larval midgut. The enzyme was localized in the soluble fraction of posterior midgut tissues, corresponding to a predicted cytoplasmic localization of prolidase according to the structure of the mRNA transcript. Expression of genes encoding prolidase and the major digestive proline-specific peptidase (PSP)-dipeptidyl peptidase 4-were similar. The pH optimum of T. molitor prolidase was 7.5, and the enzyme was inhibited by Z-Pro, indicating that it belongs to type I prolidases. In mammals, prolidase is particularly important in the catabolism of a proline-rich protein-collagen. We propose that T. molitor larval prolidase is a critical enzyme for the final stages of digestion of dietary proline-rich gliadins, providing hydrolysis of imidodipeptides in the cytoplasm of midgut epithelial cells. We propose that the products of hydrolysis are absorbed from the luminal contents by peptide transporters, which we have annotated in the T. molitor larval gut transcriptome. The origin of prolidase substrates in the insect midgut is discussed in the context of overall success of grain feeding insects.
[Mh] Termos MeSH primário: Dipeptidases/metabolismo
Gliadina/metabolismo
Proteínas de Insetos/metabolismo
Tenebrio/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Dipeptidases/antagonistas & inibidores
Dipeptidases/isolamento & purificação
Trato Gastrointestinal/enzimologia
Proteínas de Insetos/antagonistas & inibidores
Proteínas de Insetos/isolamento & purificação
Larva/enzimologia
Proteínas de Membrana Transportadoras/metabolismo
Especificidade por Substrato
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Membrane Transport Proteins); 9007-90-3 (Gliadin); 97599-47-8 (peptide permease); EC 3.4.13.- (Dipeptidases); EC 3.4.13.9 (proline dipeptidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE
[do] DOI:10.1002/arch.21395


  8 / 806 MEDLINE  
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[PMID]:28429650
[Au] Autor:Debode F; Marien A; Gérard A; Francis F; Fumière O; Berben G
[Ad] Endereço:a Unit Traceability and Authentication , Walloon Agricultural Research Center (CRA-W) , Gembloux , Belgium.
[Ti] Título:Development of real-time PCR tests for the detection of Tenebrio molitor in food and feed.
[So] Source:Food Addit Contam Part A Chem Anal Control Expo Risk Assess;34(8):1421-1426, 2017 Aug.
[Is] ISSN:1944-0057
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Insects are rich in proteins and could be an alternative source of proteins to feed animals and humans. Numerous companies have started the production of insects for feed purposes. In Europe, these processed animal proteins are not yet authorised by legislation as many questions still need to be answered concerning this 'novel food'. Authorisations will be possible when methods of authentication of the products are available. In this study we propose real-time PCR methods for the specific detection of the mealworm (Tenebriomolitor), one of the most widely used insects for food and feed production. Two PCR assays are proposed: the first based on the wingless gene and the second based on the cadherin gene. The PCR tests amplify fragments of 87 bp. These qualitative methods were tested according to several performance criteria. The specificity was tested on 34 insect species' DNA, but also on non-insect species including crustacean, mammals, birds and plants. The limit of detection was determined and was below 20 copies for the two PCR tests. The applicability of the tests was demonstrated by the analysis of real-life processed samples containing T. molitor.
[Mh] Termos MeSH primário: Ração Animal/análise
Análise de Alimentos
Reação em Cadeia da Polimerase em Tempo Real
Tenebrio/genética
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.1080/19440049.2017.1320811


  9 / 806 MEDLINE  
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[PMID]:28423415
[Au] Autor:Jiang M; Lü S; Zhang Y
[Ad] Endereço:Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China (mingjiang@nwsuaf.edu.cn; shuminlv@nwsuaf.edu.cn; yalinzh@nwsuaf.edu.cn).
[Ti] Título:The Potential Organ Involved in Cantharidin Biosynthesis in Epicauta chinensis Laporte (Coleoptera: Meloidae).
[So] Source:J Insect Sci;17(2), 2017 Jan 01.
[Is] ISSN:1536-2442
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cantharidin, a terpenoid defensive toxin mainly produced by blister beetles, is among the most widely known insect natural products in the world. However, little is known about the site of cantharidin biosynthesis in vivo. Our previous research showed that 3-hydroxy-3-methylglutary-CoA reductase (HMGR) is an essential enzyme in cantharidin biosynthesis. In this report, we further investigated cantharidin titer and HMGR mRNA expression levels in different tissues of male and female Epicauta chinensis, and performed a comparative analysis of HMGR transcript levels in male Tenebrio molitor, a Tenebrionidae beetle that cannot produce cantharidin. HMGR transcripts had a positive correlation with cantharidin production. Furthermore, the specifically high amounts of HMGR transcript and abundant cantharidin production in fat body of male E. chinensis indicated the process of cantharidin synthesis may occur in the fat body.
[Mh] Termos MeSH primário: Cantaridina/metabolismo
Coleópteros/genética
Corpo Adiposo/metabolismo
Hidroximetilglutaril-CoA Redutases/genética
Proteínas de Insetos/genética
Transcrição Genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Coleópteros/metabolismo
DNA Complementar/genética
DNA Complementar/metabolismo
Feminino
Hidroximetilglutaril-CoA Redutases/química
Hidroximetilglutaril-CoA Redutases/metabolismo
Proteínas de Insetos/química
Proteínas de Insetos/metabolismo
Masculino
Especificidade de Órgãos
Filogenia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Alinhamento de Sequência
Tenebrio/genética
Tenebrio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Insect Proteins); 0 (RNA, Messenger); EC 1.1.1.- (Hydroxymethylglutaryl CoA Reductases); IGL471WQ8P (Cantharidin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1093/jisesa/iex021


  10 / 806 MEDLINE  
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[PMID]:28264489
[Au] Autor:Seo M; Goo TW; Chung MY; Baek M; Hwang JS; Kim MA; Yun EY
[Ad] Endereço:Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA, Wanju-gun 55365, Korea. nansmc@hanmail.net.
[Ti] Título:Tenebrio molitor Larvae Inhibit Adipogenesis through AMPK and MAPKs Signaling in 3T3-L1 Adipocytes and Obesity in High-Fat Diet-Induced Obese Mice.
[So] Source:Int J Mol Sci;18(3), 2017 Feb 28.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Despite the increasing interest in insect-based bioactive products, the biological activities of these products are rarely studied adequately. Larvae of , the yellow mealworm, have been eaten as a traditional food and provide many health benefits. Therefore, we hypothesized that larvae might influence adipogenesis and obesity-related disorders. In the present study, we investigated the anti-adipogenic and antiobesity effects of larvae in vitro and in vivo. The lipid accumulation and triglyceride content in mature adipocytes was reduced significantly (up to 90%) upon exposure to an ethanol extract of larvae, without a reduction in cell viability. Exposure also resulted in key adipogenic and lipogenic transcription factors. Additionally, in adipogenic differentiation medium the extract induced phosphorylation of adenosine monophosphate (AMP)-activated protein kinase and mitogen-activated protein kinases. Daily oral administration of larvae powder to obese mice fed high-fat diet attenuated body weight gain. We also found that the powder efficiently reduced hepatic steatosis as well as aspartate and alanine transaminase enzyme levels in mice fed a high-fat diet. Our results suggest that larvae extract has an antiobesity effect when administered as a food supplement and has potential as a therapeutic agent for obesity.
[Mh] Termos MeSH primário: Proteínas Quinases Ativadas por AMP/metabolismo
Adipócitos/metabolismo
Adipogenia/efeitos dos fármacos
Produtos Biológicos/administração & dosagem
Larva
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Obesidade/metabolismo
Transdução de Sinais/efeitos dos fármacos
Tenebrio
[Mh] Termos MeSH secundário: Células 3T3-L1
Adipócitos/citologia
Adipócitos/efeitos dos fármacos
Adipogenia/genética
Tecido Adiposo/anatomia & histologia
Tecido Adiposo/efeitos dos fármacos
Animais
Fármacos Antiobesidade/administração & dosagem
Peso Corporal/efeitos dos fármacos
Diferenciação Celular/efeitos dos fármacos
Dieta Hiperlipídica
Suplementos Nutricionais
Modelos Animais de Doenças
Fígado Gorduroso/tratamento farmacológico
Fígado Gorduroso/etiologia
Fígado Gorduroso/metabolismo
Regulação da Expressão Gênica/efeitos dos fármacos
Masculino
Camundongos
Camundongos Obesos
Obesidade/tratamento farmacológico
Obesidade/etiologia
Fosforilação
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Obesity Agents); 0 (Biological Products); 0 (Transcription Factors); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); EC 2.7.11.31 (AMP-Activated Protein Kinases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE



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