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Pesquisa : B01.050.500.131.617.720.500.500.375.799 [Categoria DeCS]
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  1 / 1060 MEDLINE  
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[PMID]:29265467
[Au] Autor:Guan J; Zhang J; Yuan S; Yang B; Clark KD; Ling E; Huang W
[Ad] Endereço:Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
[Ti] Título:Analysis of the functions of the signal peptidase complex in the midgut of Tribolium castaneum.
[So] Source:Arch Insect Biochem Physiol;97(3), 2018 Mar.
[Is] ISSN:1520-6327
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Signal peptidase complexes (SPCs) are conserved from bacteria to human beings, and are typically composed of four to five subunits. There are four genes encoding SPC proteins in the red flour beetle, Tribolium castaneum. To understand their importance to insect development, double-stranded RNA for each SPC gene was injected into red flour beetles at the early larval and adult stages. Knockdown of all four signal peptidase genes was lethal to larvae. Moreover, larvae had difficulty with old cuticle ecdysis. Knockdown of TcSPC12 alone did not affect pupal or adult development. When TcSPC12, TcSPC18, and TcSPC25 were knocked down in larvae, the melanization of hemocytes and midguts was observed. When knocked down in larvae and adults, TcSPC18 induced severe cell apoptosis in midguts, and the adult midgut lost the ability to maintain crypts after knockdown of TcSPC18, indicating its importance to midgut cell proliferation and differentiation. Knockdown of TcSPC22 or TcSPC25 also resulted in many apoptotic cells in the midguts. However, TcSPC12 appeared to be unimportant for midgut development. We conclude that TcSPC18 is essential for maintaining the adult midgut crypts.
[Mh] Termos MeSH primário: Proteínas de Membrana/metabolismo
Serina Endopeptidases/metabolismo
Tribolium/enzimologia
[Mh] Termos MeSH secundário: Animais
Feminino
Trato Gastrointestinal/enzimologia
Hemócitos/metabolismo
Proteínas de Insetos/metabolismo
Melaninas/metabolismo
Interferência de RNA
Tribolium/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Melanins); 0 (Membrane Proteins); EC 3.4.21.- (Serine Endopeptidases); EC 3.4.21.89 (type I signal peptidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1002/arch.21441


  2 / 1060 MEDLINE  
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[PMID]:28747473
[Au] Autor:Tate AT; Graham AL
[Ad] Endereço:Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA a.tate@vanderbilt.edu.
[Ti] Título:Dissecting the contributions of time and microbe density to variation in immune gene expression.
[So] Source:Proc Biol Sci;284(1859), 2017 Jul 26.
[Is] ISSN:1471-2954
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Widespread differential expression of immunological genes is a hallmark of the response to infection in almost all surveyed taxa. However, several challenges remain in the attempt to connect differences in gene expression with functional outcomes like parasite killing and host survival. For example, temporal gene expression patterns are not always monotonic (unidirectional slope), yielding results that qualitatively depend on the time point selected for analysis. They may also be correlated to microbe density, confounding the strength of an immune response and resistance to parasites. In this study, we analyse these relationships in an mRNA-seq time series of infected with Our results suggest that many extracellular immunological components with known roles in immunity, like antimicrobial peptides and recognition proteins, are highly correlated to microbe load. On the other hand, intracellular components of immunological signalling pathways overwhelmingly show non-monotonic temporal patterns of gene expression, despite the underlying assumption of monotonicity in most ecological and comparative transcriptomics studies that rely on cross-sectional analyses. Our results raise a host of new questions, including to what extent variation in host resistance, infection tolerance and immunopathology can be explained by variation in the slope or sensitivity of these newly characterized patterns.
[Mh] Termos MeSH primário: Carga Bacteriana
Regulação da Expressão Gênica/imunologia
Tribolium/imunologia
[Mh] Termos MeSH secundário: Animais
Bacillus thuringiensis/patogenicidade
Estudos Transversais
Transdução de Sinais
Fatores de Tempo
Tribolium/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE


  3 / 1060 MEDLINE  
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[PMID]:28449092
[Au] Autor:Song X; Huang F; Liu J; Li C; Gao S; Wu W; Zhai M; Yu X; Xiong W; Xie J; Li B
[Ad] Endereço:Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
[Ti] Título:Genome-wide DNA methylomes from discrete developmental stages reveal the predominance of non-CpG methylation in Tribolium castaneum.
[So] Source:DNA Res;24(5):445-457, 2017 Oct 01.
[Is] ISSN:1756-1663
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cytosine DNA methylation is a vital epigenetic regulator of eukaryotic development. Whether this epigenetic modification occurs in Tribolium castaneum has been controversial, its distribution pattern and functions have not been established. Here, using bisulphite sequencing (BS-Seq), we confirmed the existence of DNA methylation and described the methylation profiles of the four life stages of T. castaneum. In the T. castaneum genome, both symmetrical CpG and non-CpG methylcytosines were observed. Symmetrical CpG methylation, which was catalysed by DNMT1 and occupied a small part in T. castaneum methylome, was primarily enriched in gene bodies and was positively correlated with gene expression levels. Asymmetrical non-CpG methylation, which was predominant in the methylome, was strongly concentrated in intergenic regions and introns but absent from exons. Gene body methylation was negatively correlated with gene expression levels. The distribution pattern and functions of this type of methylation were similar only to the methylome of Drosophila melanogaster, which further supports the existence of a novel methyltransferase in the two species responsible for this type of methylation. This first life-cycle methylome of T. castaneum reveals a novel and unique methylation pattern, which will contribute to the further understanding of the variety and functions of DNA methylation in eukaryotes.
[Mh] Termos MeSH primário: Metilação de DNA
Epigênese Genética
Genoma de Inseto
Tribolium/genética
[Mh] Termos MeSH secundário: Animais
DNA/metabolismo
DNA (Citosina-5-)-Metiltransferases/metabolismo
Drosophila melanogaster/genética
Drosophila melanogaster/metabolismo
Tribolium/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA); EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1093/dnares/dsx016


  4 / 1060 MEDLINE  
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[PMID]:27770578
[Au] Autor:Perkin LC; Elpidina EN; Oppert B
[Ad] Endereço:USDA, Agricultural Research Service, Center for Grain and Animal Health Research, 1515 College Avenue, Manhattan, KS, USA.
[Ti] Título:RNA interference and dietary inhibitors induce a similar compensation response in Tribolium castaneum larvae.
[So] Source:Insect Mol Biol;26(1):35-45, 2017 02.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tribolium castaneum is a major agriculture pest damaging stored grains and cereal products. The T. castaneum genome contains 26 cysteine peptidase genes, mostly cathepsins L and B, and seven have a major role in digestion. We targeted the expression of the most highly expressed cathepsin L gene on chromosome 10, TC011001, by RNA interference (RNAi), using double-stranded RNA (dsRNA) constructs of different regions of the gene (3', middle, 5' and entire coding regions). RNA sequencing and quantitation (RNA-seq) was used to evaluate knockdown and specificity amongst the treatments. Overall, target gene expression decreased in all treatment groups, but was more severe and specific in dsRNA targeting the 3' and entire coding regions, encoding the proteolytic active site in the enzyme. Additional cysteine cathepsin genes also were down-regulated (off-target effects), but some were up-regulated in response to RNAi treatment. Notably, some serine peptidase genes were increased in expression, especially in dsRNA targeting 5' and middle regions, and the response was similar to the effects of dietary cysteine protease inhibitors. We manually annotated these serine peptidase genes to gain insight into function and relevance to the RNAi study. The data indicate that T. castaneum larvae compensate for the loss of digestive peptidase activity in the larval gut, regardless of the mechanism of disruption.
[Mh] Termos MeSH primário: Catepsina L/genética
Interferência de RNA
Tribolium/genética
[Mh] Termos MeSH secundário: Animais
Catepsina L/metabolismo
Cisteína Proteases/metabolismo
Larva/metabolismo
RNA de Cadeia Dupla
Serina Proteases/metabolismo
Transcriptoma
Tribolium/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Double-Stranded); EC 3.4.- (Cysteine Proteases); EC 3.4.- (Serine Proteases); EC 3.4.22.15 (Cathepsin L)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12269


  5 / 1060 MEDLINE  
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[PMID]:29023543
[Au] Autor:Takada T; Sato R; Kikuta S
[Ad] Endereço:Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan.
[Ti] Título:A mannitol/sorbitol receptor stimulates dietary intake in Tribolium castaneum.
[So] Source:PLoS One;12(10):e0186420, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In insects, perception of chemical stimuli is involved in the acceptance or rejection of food. Gustatory receptors (Grs) that regulate external signals in chemosensory organs have been found in many insects. Tribolium castaneum, a major pest of stored products, possesses over 200 Gr genes. An expanded repertoire of Gr genes appears to be required for diet recognition in species that are generalist feeders; however, it remains unclear whether T. castaneum recognizes a suite of chemicals common to many products or whether its feeding is activated by specific chemicals, and whether its Grs are involved in feeding behavior. It is difficult to determine the food preferences of T. castaneum based on dietary intake due to a lack of appropriate methodology. This study established a novel dietary intake estimation method using gypsum, designated the TribUTE (Tribolium Urges To Eat) assay. For this assay, T. castaneum adults were fed a gypsum block without added organic compounds. Sweet preference was determined by adding sweeteners and measuring the amount of gypsum in the excreta. Mannitol was the strongest activator of T. castaneum dietary intake. In a Xenopus oocyte expression, TcGr20 was found to be responsible for mannitol and sorbitol responses, but not for responses to other tested non-volatile compounds. The EC50 values of TcGr20 for mannitol and sorbitol were 72.6 mM and 90.6 mM, respectively, suggesting that TcGr20 is a feasible receptor for the recognition of mannitol at lower concentrations. We used RNAi and the TribUTE assay to examine whether TcGr20 expression was involved in mannitol recognition. The amounts of excreta in TcGr20 dsRNA-injected adults decreased significantly, despite the presence of mannitol, compared to control adults. Taken together, our results indicate that T. castaneum adults recognized mannitol/sorbitol using the TcGr20 receptor, thereby facilitating the dietary intake of these compounds.
[Mh] Termos MeSH primário: Dieta
Proteínas de Insetos/metabolismo
Receptores de Superfície Celular/metabolismo
Tribolium/fisiologia
[Mh] Termos MeSH secundário: Animais
Expressão Gênica/efeitos dos fármacos
Proteínas de Insetos/antagonistas & inibidores
Proteínas de Insetos/classificação
Proteínas de Insetos/genética
Manitol/farmacologia
Oócitos/efeitos dos fármacos
Oócitos/fisiologia
Técnicas de Patch-Clamp
Filogenia
Interferência de RNA
RNA de Cadeia Dupla/metabolismo
Receptores de Superfície Celular/antagonistas & inibidores
Receptores de Superfície Celular/classificação
Receptores de Superfície Celular/genética
Sorbitol/farmacologia
Tribolium/genética
Xenopus/crescimento & desenvolvimento
Xenopus/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (RNA, Double-Stranded); 0 (Receptors, Cell Surface); 3OWL53L36A (Mannitol); 506T60A25R (Sorbitol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171105
[Lr] Data última revisão:
171105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186420


  6 / 1060 MEDLINE  
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[PMID]:29016664
[Au] Autor:Cepeda RE; Pardo RV; Macaya CC; Sarrazin AF
[Ad] Endereço:Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
[Ti] Título:Contribution of cell proliferation to axial elongation in the red flour beetle Tribolium castaneum.
[So] Source:PLoS One;12(10):e0186159, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Most arthropods generate their posterior bodies by adding segments periodically, as the embryo grows, from a posteriorly located region called the segment addition zone. This mode of segmentation is shared with vertebrates and relies on oscillatory mechanisms, where the temporal periodicity of a clock is translated into repetitive spatial patterns. This ordered anterior-to-posterior pattern is achieved at the same time as the tissue elongates, opening the question of the functional coordination between the mechanisms of segmental patterning and posterior growth. The study of these processes in different arthropods has played an important role in unravelling some of the molecular mechanisms of segment formation. However, the behavior of cells during elongation and how cellular processes affect this segmental patterning has been poorly studied. Cell proliferation together with cell rearrangements are presumed to be the major forces driving axis elongation in the red flour beetle Tribolium castaneum. However, there still no strong evidence about the role and distribution of cell proliferation within the embryo. In this study, we propose to address these questions by using whole embryo cultures and pharmacological manipulation. We show that considerable cell proliferation occurs during germband elongation, measured by incorporation of the nucleoside analog of thymidine 5-Ethynyl-2'-deoxyuridine, EdU. Moreover, proliferating cells appeared to be spread along the elongating embryo with a posterior bias at early segmentation. In addition, when we blocked cell division, treated germbands were always shorter than controls and in some cases not able to fully elongate, even when control embryos already started to retract and leg buds are evident. Finally, we found that the absence of cell proliferation has no apparent effect on segmental patterning, as evidenced by Tc-engrailed (Tc-en) gene expression.
[Mh] Termos MeSH primário: Padronização Corporal/genética
Proliferação Celular/genética
Tribolium/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Padronização Corporal/fisiologia
Embrião não Mamífero
Regulação da Expressão Gênica no Desenvolvimento
Proteínas de Insetos/genética
Nucleosídeos/metabolismo
Tribolium/embriologia
Tribolium/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Nucleosides)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186159


  7 / 1060 MEDLINE  
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[PMID]:28949983
[Au] Autor:Yan Y; Williams SB; Murdock LL; Baributsa D
[Ad] Endereço:Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, United States of America.
[Ti] Título:Hermetic storage of wheat and maize flour protects against red flour beetle (Tribolium castaneum Herbst).
[So] Source:PLoS One;12(9):e0185386, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hermetic storage is used to protect grain against insect pests, but its utility is not limited to whole grains. We evaluated hermetically-sealed, polyethylene terephthalate (PET) bottles for preserving wheat and maize flour against red flour beetle (RFB, Tribolium castaneum, Herbst) population growth. Flours infested with RFB and kept in sealed PET bottles experienced much less weight loss over a three-month storage period than infested flour kept in unsealed bottles. RFB populations in wheat flour kept in sealed bottles did not increase, while populations in unsealed bottles grew about 50-fold during the same three-month period. Flour in sealed bottles had lower levels of oxygen and moisture than flour stored in unsealed bottles. Similar trends were observed for oxygen and moisture levels in maize flour held in hermetically sealed bottles. Hermetically-sealed bottles were effective in preventing RFB population growth and preserving maize and wheat flour. Farmers, consumers and food processors can safely store grain flour in hermetic sealed containers.
[Mh] Termos MeSH primário: Armazenamento de Alimentos
Tribolium/crescimento & desenvolvimento
Triticum
Zea mays
[Mh] Termos MeSH secundário: Animais
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185386


  8 / 1060 MEDLINE  
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[PMID]:28811313
[Au] Autor:Kitzmann P; Weißkopf M; Schacht MI; Bucher G
[Ad] Endereço:Department of Evolutionary Developmental Genetics, GZMB, Universität Göttingen, Justus von Liebig Weg 11, 37077 Göttingen, Germany.
[Ti] Título:A key role for in anterior head and central brain patterning in insects.
[So] Source:Development;144(16):2969-2981, 2017 08 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Anterior patterning of animals is based on a set of highly conserved transcription factors but the interactions within the protostome anterior gene regulatory network (aGRN) remain enigmatic. Here, we identify the red flour beetle ortholog of ( ) as a novel upstream component of the aGRN. It is required for the development of the labrum and higher order brain structures, namely the central complex and the mushroom bodies. We reveal interactions by RNAi and heat shock-mediated misexpression. Surprisingly, and mutually activate each other, forming a novel regulatory module at the top of the aGRN. Comparisons of our results with those of sea urchins and cnidarians suggest that has acquired more upstream functions in the aGRN during protostome evolution. Our findings expand the knowledge on gene function to include essential roles in epidermal development and central brain patterning.
[Mh] Termos MeSH primário: Proteínas de Insetos/metabolismo
Tribolium/embriologia
[Mh] Termos MeSH secundário: Animais
Padronização Corporal/genética
Padronização Corporal/fisiologia
Cnidários/metabolismo
Regulação da Expressão Gênica no Desenvolvimento/genética
Regulação da Expressão Gênica no Desenvolvimento/fisiologia
Proteínas de Insetos/genética
Ouriços-do-Mar/metabolismo
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
Tribolium/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170817
[St] Status:MEDLINE
[do] DOI:10.1242/dev.147637


  9 / 1060 MEDLINE  
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[PMID]:28800588
[Au] Autor:Strobl F; Ross JA; Stelzer EHK
[Ad] Endereço:Physical Biology / Physikalische Biologie (IZN, FB 15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt-Macromolecular Complexes (CEF-MC), Goethe Universität-Frankfurt am Main (Campus Riedberg), Max-von-Laue-Straße 15, Frankfurt am Main-Germany.
[Ti] Título:Non-lethal genotyping of Tribolium castaneum adults using genomic DNA extracted from wing tissue.
[So] Source:PLoS One;12(8):e0182564, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The red flour beetle Tribolium castaneum has become the second most important insect model organism and is frequently used in developmental biology, genetics and pest-associated research. Consequently, the methodological arsenal increases continuously, but many routinely applied techniques for Drosophila melanogaster and other insect species are still unavailable. For example, a protocol for non-lethal genotyping has not yet been adapted but is particularly useful when individuals with known genotypes are required for downstream experiments. In this study, we present a workflow for non-lethal genotyping of T. castaneum adults based on extracting genomic DNA from wing tissue. In detail, we describe a convenient procedure for wing dissection and a custom method for wing digestion that allows PCR-based genotyping of up to fifty adults in less than an afternoon with a success rate of about 86%. The amount of template is sufficient for up to ten reactions while viability and fertility of the beetles are preserved. We prove the applicability of our protocol by genotyping the white / scarlet gene pair alleles from the black-eyed San Bernadino wild-type and white-eyed Pearl recessive mutant strains spanning four generations. Non-lethal genotyping has the potential to improve and accelerate many workflows: Firstly, during the establishment process of homozygous cultures or during stock keeping of cultures that carry recessively lethal alleles, laborious test crossing is replaced by non-lethal genotyping. Secondly, in genome engineering assays, non-lethal genotyping allows the identification of appropriate founders before they are crossed against wild-types, narrowing the efforts down to only the relevant individuals. Thirdly, non-lethal genotyping simplifies experimental strategies, in which genotype and behavior should be correlated, since the genetic configuration of potential individuals can be determined before the actual behavior assays is performed.
[Mh] Termos MeSH primário: Genótipo
Técnicas de Genotipagem
Tribolium/genética
Asas de Animais/química
[Mh] Termos MeSH secundário: Animais
Sondas de DNA/síntese química
Genes Letais
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Probes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182564


  10 / 1060 MEDLINE  
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[PMID]:28786992
[Au] Autor:Lü J; Liu S
[Ad] Endereço:Province Key Laboratory of Transformation and Utilization of Cereal Resource, School of Food Science and Technology, Henan University of Technology, Zhengzhou High-Tech Development Zone, Zhengzhou, Henan, China.
[Ti] Título:Influence of acclimation to sublethal temperature on heat tolerance of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) exposed to 50°C.
[So] Source:PLoS One;12(8):e0182269, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) is a serious pest of stored agricultural products and one of the most common insects found in grain storage and food processing facilities. Heat treatment has been revisited to control stored-product insects as a potential alternative to methyl bromide for disinfesting mills and food-processing facilities. The influence of acclimation of T. castaneum adults, pupae, larvae, and eggs to sublethal temperatures of 36, and 42°C on their subsequent susceptibility to lethal temperature of 50°C was respectively investigated. The acclimation of T. castaneum eggs, larvae, pupae, and adults to 36, and 42°C significantly decreased their subsequent susceptibility to lethal high temperature of 50°C. The influence of acclimation to 42°C was significantly greater than that of acclimation to 36°C. The most influential acclimation times at 42°C for mortality of T. castaneum eggs, larvae, pupae, and adults were 15, 5, 5, and 5 h, respectively, and their corresponding mortality were 41.24, 5.59, 20.19, and 4.48%, compared to 100% mortality of T. castaneum eggs, larvae, pupae, and adults without acclimation when exposed to 50°C for 35 min, respectively. The present results have important implications for developing successful heat treatment protocols to control T. castaneum, improving disinfestation effectiveness of heat treatment and understanding insect response to high temperatures.
[Mh] Termos MeSH primário: Temperatura Alta
Termotolerância/fisiologia
Tribolium/fisiologia
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Regulação da Expressão Gênica/fisiologia
Proteínas de Choque Térmico HSP70/metabolismo
Proteínas de Insetos/metabolismo
Distribuição Aleatória
Canais de Cátion TRPC/metabolismo
Fatores de Tempo
Tribolium/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HSP70 Heat-Shock Proteins); 0 (Insect Proteins); 0 (TRPC Cation Channels)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182269



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