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  1 / 6526 MEDLINE  
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[PMID]:29240785
[Au] Autor:Lu W; Hwang JK; Zeng F; Leal WS
[Ad] Endereço:Department of Molecular and Cellular Biology, University of California Davis, Davis, CA, United States of America.
[Ti] Título:DEET as a feeding deterrent.
[So] Source:PLoS One;12(12):e0189243, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The insect repellent N,N-diethyl-3-methylbenzamide (DEET), is a multimodal compound that acts as a spatial repellent as well as an irritant (contact repellent), thus being perceived by the insect's olfactory and gustatory systems as an odorant and a tastant, respectively. Soon after DEET was developed, almost 6 decades ago, it was reported that it reduced mosquito feeding on blood mixed with this repellent. It is now known that the mosquito proboscis senses contact repellents with the tips (labella) of the labium, which remain in direct contact with the outer layers of the skin, while the stylets, including the feeding deterrent sensor (labrum), penetrate the skin. We designed a behavioral assay that allowed us to measure feeding deterrence without complications from contact or spatial repellency. Using the southern house mosquito, Culex quinquefasciatus, we demonstrate here that when DEET was mixed with blood and covered by Parafilm® layers, the mean number of landings and duration of contacts with surfaces covering blood mixed with DEET or blood plus solvent (dimethyl sulfoxide) did not differ significantly thus implying that DEET did not leak to the outer surface. The feeding times, however, were significantly different. When blood was mixed either with 0.1 or 1% DEET, female southern house mosquitoes spent significantly (P<0.0001) less time feeding than the time spent feeding on blood mixed only with the solvent. By contrast, significant differences in the mean times of feeding on blood containing 1% picaridin and blood plus solvent were significant at 5%, but not at 1% level. Like DEET, the contact repellent and insecticide, permethrin, caused a significant (P<0.0001) reduction in feeding time. We, therefore, concluded, that in this context, DEET, permethrin, and, to a lesser extent, picaridin, act as feeding deterrents.
[Mh] Termos MeSH primário: Culex/efeitos dos fármacos
DEET/farmacologia
Comportamento Alimentar/efeitos dos fármacos
Repelentes de Insetos/farmacologia
[Mh] Termos MeSH secundário: Animais
Culex/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Repellents); 134-62-3 (DEET)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189243


  2 / 6526 MEDLINE  
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[PMID]:29385205
[Au] Autor:Di Paola N; Freire CCM; Zanotto PMA
[Ad] Endereço:Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute, University of Sao Paulo, Sao Paulo, Brazil.
[Ti] Título:Does adaptation to vertebrate codon usage relate to flavivirus emergence potential?
[So] Source:PLoS One;13(1):e0191652, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Codon adaptation index (CAI) is a measure of synonymous codon usage biases given a usage reference. Through mutation, selection, and drift, viruses can optimize their replication efficiency and produce more offspring, which could increase the chance of secondary transmission. To evaluate how higher CAI towards the host has been associated with higher viral titers, we explored temporal trends of several historic and extensively sequenced zoonotic flaviviruses and relationships within the genus itself. To showcase evolutionary and epidemiological relationships associated with silent, adaptive synonymous changes of viruses, we used codon usage tables from human housekeeping and antiviral immune genes, as well as tables from arthropod vectors and vertebrate species involved in the flavivirus maintenance cycle. We argue that temporal trends of CAI changes could lead to a better understanding of zoonotic emergences, evolutionary dynamics, and host adaptation. CAI appears to help illustrate historically relevant trends of well-characterized viruses, in different viral species and genetic diversity within a single species. CAI can be a useful tool together with in vivo and in vitro kinetics, phylodynamics, and additional functional genomics studies to better understand species trafficking and viral emergence in a new host.
[Mh] Termos MeSH primário: Códon/genética
Flavivirus/genética
Flavivirus/patogenicidade
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Aedes/genética
Aedes/virologia
Animais
Culex/genética
Culex/virologia
Vírus da Dengue/genética
Vírus da Dengue/patogenicidade
Vírus da Dengue/fisiologia
Evolução Molecular
Flavivirus/fisiologia
Genes Essenciais
Genoma Viral
Interações Hospedeiro-Patógeno/genética
Seres Humanos
Mosquitos Vetores/genética
Mosquitos Vetores/virologia
Filogenia
Vírus do Mosaico do Tabaco/genética
Vírus do Mosaico do Tabaco/patogenicidade
Vírus do Mosaico do Tabaco/fisiologia
Vertebrados/genética
Vertebrados/virologia
Vírus do Nilo Ocidental/genética
Vírus do Nilo Ocidental/patogenicidade
Vírus do Nilo Ocidental/fisiologia
Vírus da Febre Amarela/genética
Vírus da Febre Amarela/patogenicidade
Vírus da Febre Amarela/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Codon)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191652


  3 / 6526 MEDLINE  
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[PMID]:29358578
[Au] Autor:Bonneau M; Atyame C; Beji M; Justy F; Cohen-Gonsaud M; Sicard M; Weill M
[Ad] Endereço:Institut des Sciences de l'Evolution de Montpellier (ISEM), UMR CNRS-IRD-EPHE-Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, France.
[Ti] Título:Culex pipiens crossing type diversity is governed by an amplified and polymorphic operon of Wolbachia.
[So] Source:Nat Commun;9(1):319, 2018 01 22.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Culex pipiens mosquitoes are infected with Wolbachia (wPip) that cause an important diversity of cytoplasmic incompatibilities (CIs). Functional transgenic studies have implicated the cidA-cidB operon from wPip and its homolog in wMel in CI between infected Drosophila males and uninfected females. However, the genetic basis of the CI diversity induced by different Wolbachia strains was unknown. We show here that the remarkable diversity of CI in the C. pipiens complex is due to the presence, in all tested wPip genomes, of several copies of the cidA-cidB operon, which undergoes diversification through recombination events. In 183 isofemale lines of C. pipiens collected worldwide, specific variations of the cidA-cidB gene repertoires are found to match crossing types. The diversification of cidA-cidB is consistent with the hypothesis of a toxin-antitoxin system in which the gene cidB co-diversifies with the gene cidA, particularly in putative domains of reciprocal interactions.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Culex/microbiologia
Drosophila melanogaster/microbiologia
Genoma Bacteriano
Óperon
Wolbachia/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas de Bactérias/metabolismo
Sequência de Bases
Cruzamentos Genéticos
Culex/genética
Drosophila melanogaster/genética
Feminino
Especificidade de Hospedeiro
Masculino
Polimorfismo Genético
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Simbiose/genética
Sistemas Toxina-Antitoxina/genética
Wolbachia/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02749-w


  4 / 6526 MEDLINE  
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[PMID]:29342169
[Au] Autor:Reeves LE; Krysko KL; Avery ML; Gillett-Kaufman JL; Kawahara AY; Connelly CR; Kaufman PE
[Ad] Endereço:Entomology and Nematology Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida, United States of America.
[Ti] Título:Interactions between the invasive Burmese python, Python bivittatus Kuhl, and the local mosquito community in Florida, USA.
[So] Source:PLoS One;13(1):e0190633, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Burmese python, Python bivittatus Kuhl, is a well-established invasive species in the greater Everglades ecosystem of southern Florida, USA. Most research on its ecological impacts focuses on its role as a predator and its trophic interactions with native vertebrate species, particularly mammals. Beyond predation, there is little known about the ecological interactions between P. bivittatus and native faunal communities. It is likely that established populations of P. bivittatus in southern Florida serve as hosts for native mosquito communities. To test this concept, we used mitochondrial cytochrome c oxidase subunit I DNA barcoding to determine the hosts of blood fed mosquitoes collected at a research facility in northern Florida where captive P. bivittatus and Argentine black and white tegu, Salvator merianae (Duméril and Bibron), are maintained in outdoor enclosures, accessible to local mosquitoes. We recovered python DNA from the blood meals of three species of Culex mosquitoes: Culex erraticus (Dyar and Knab), Culex quinquefasciatus Say, and Culex pilosus (Dyar and Knab). Culex erraticus conclusively (P = 0.001; Fisher's Exact Test) took more blood meals from P. bivittatus than from any other available host. While the majority of mosquito blood meals in our sample were derived from P. bivittatus, only one was derived from S. merianae. These results demonstrate that local mosquitoes will feed on invasive P. bivittatus, a recently introduced host. If these interactions also occur in southern Florida, P. bivittatus may be involved in the transmission networks of mosquito-vectored pathogens. Our results also illustrate the potential of detecting the presence of P. bivittatus in the field through screening mosquito blood meals for their DNA.
[Mh] Termos MeSH primário: Boidae/fisiologia
Culex
Interações Hospedeiro-Parasita
Espécies Introduzidas
[Mh] Termos MeSH secundário: Animais
Feminino
Florida
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190633


  5 / 6526 MEDLINE  
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[PMID]:29281642
[Au] Autor:Vloet RPM; Vogels CBF; Koenraadt CJM; Pijlman GP; Eiden M; Gonzales JL; van Keulen LJM; Wichgers Schreur PJ; Kortekaas J
[Ad] Endereço:Department of Virology, Wageningen Bioveterinary Research, Lelystad, the Netherlands.
[Ti] Título:Transmission of Rift Valley fever virus from European-breed lambs to Culex pipiens mosquitoes.
[So] Source:PLoS Negl Trop Dis;11(12):e0006145, 2017 12.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Rift Valley fever virus (RVFV) is a mosquito-borne bunyavirus of the genus Phlebovirus that is highly pathogenic to ruminants and humans. The disease is currently confined to Africa and the Arabian Peninsula, but globalization and climate change may facilitate introductions of the virus into currently unaffected areas via infected animals or mosquitoes. The consequences of such an introduction will depend on environmental factors, the availability of susceptible ruminants and the capacity of local mosquitoes to transmit the virus. We have previously demonstrated that lambs native to the Netherlands are highly susceptible to RVFV and we here report the vector competence of Culex (Cx.) pipiens, the most abundant and widespread mosquito species in the country. Vector competence was first determined after artificial blood feeding of laboratory-reared mosquitoes using the attenuated Clone 13 strain. Subsequently, experiments with wild-type RVFV and mosquitoes hatched from field-collected eggs were performed. Finally, the transmission of RVFV from viremic lambs to mosquitoes was studied. PRINCIPAL FINDINGS: Artificial feeding experiments using Clone 13 demonstrated that indigenous, laboratory-reared Cx. pipiens mosquitoes are susceptible to RVFV and that the virus can be transmitted via their saliva. Experiments with wild-type RVFV and mosquitoes hatched from field-collected eggs confirmed the vector competence of Cx. pipiens mosquitoes from the Netherlands. To subsequently investigate transmission of the virus under more natural conditions, mosquitoes were allowed to feed on RVFV-infected lambs during the viremic period. We found that RVFV is efficiently transmitted from lambs to mosquitoes, although transmission was restricted to peak viremia. Interestingly, in the mosquito-exposed skin samples, replication of RVFV was detected in previously unrecognized target cells. SIGNIFICANCE: We here report the vector competence of Cx. pipiens mosquitoes from the Netherlands for RVFV. Both laboratory-reared mosquitoes and well as those hatched from field-collected eggs were found to be competent vectors. Moreover, RVFV was transmitted efficiently from indigenous lambs to mosquitoes, although the duration of host infectivity was found to be shorter than previously assumed. Interestingly, analysis of mosquito-exposed skin samples revealed previously unidentified target cells of the virus. Our findings underscore the value of including natural target species in vector competence experiments.
[Mh] Termos MeSH primário: Culex/virologia
Insetos Vetores/virologia
Febre do Vale de Rift/transmissão
Vírus da Febre do Vale do Rift/patogenicidade
Doenças dos Ovinos/transmissão
[Mh] Termos MeSH secundário: Animais
Suscetibilidade a Doenças
Feminino
Febre do Vale de Rift/virologia
Vírus da Febre do Vale do Rift/isolamento & purificação
Ovinos
Doenças dos Ovinos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006145


  6 / 6526 MEDLINE  
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[PMID]:28746985
[Au] Autor:Shahhosseini N; Chinikar S; Moosa-Kazemi SH; Sedaghat MM; Kayedi MH; Lühken R; Schmidt-Chanasit J
[Ad] Endereço:Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany.
[Ti] Título:West Nile Virus lineage-2 in Culex specimens from Iran.
[So] Source:Trop Med Int Health;22(10):1343-1349, 2017 10.
[Is] ISSN:1365-3156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Screening of mosquitoes for viruses is an important forecasting tool for emerging and re-emerging arboviruses. Iran has been known to harbour medically important arboviruses such as West Nile virus (WNV) and dengue virus (DENV) based on seroepidemiological data. However, there are no data about the potential mosquito vectors for arboviruses in Iran. This study was performed to provide mosquito and arbovirus data from Iran. MATERIALS AND METHODS: A total of 32 317 mosquitos were collected at 16 sites in five provinces of Iran in 2015 and 2016. RT-PCR for detection of flaviviruses was performed. The PCR amplicons were sequenced, and 109 WNV sequences, including one obtained in this study, were used for phylogenetic analyses. RESULTS: The 32 317 mosquito specimens belonging to 25 species were morphologically distinguished and distributed into 1222 pools. Culex pipiens s.l. comprised 56.429%. One mosquito pool (0.08%), containing 46 unfed Cx. pipiens pipiens form pipiens (Cpp) captured in August 2015, was positive for flavivirus RNA. Subsequent sequencing and phylogenetic analyses revealed that the detected Iranian WNV strain belongs to lineage 2 and clusters with a strain recently detected in humans. No flaviviruses other than WNV were detected in the mosquito pools. CONCLUSION: Cpp could be a vector for WNV in Iran. Our findings indicate recent circulation of WNV lineage-2 strain in Iran and provide a solid base for more targeted arbovirus surveillance programs.
[Mh] Termos MeSH primário: Culex/virologia
Insetos Vetores/virologia
Febre do Nilo Ocidental/transmissão
Vírus do Nilo Ocidental/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Irã (Geográfico)
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1111/tmi.12935


  7 / 6526 MEDLINE  
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[PMID]:29215274
[Au] Autor:Zhang L; Zhao G; Hu X; Liu J; Li M; Batool K; Chen M; Wang J; Xu J; Huang T; Pan X; Xu L; Yu XQ; Guan X
[Ad] Endereço:Division of Cell Biology and Biophysics, University of Missouri-Kansas City , Kansas City, Missouri 64110, United States.
[Ti] Título:Cry11Aa Interacts with the ATP-Binding Protein from Culex quinquefasciatus To Improve the Toxicity.
[So] Source:J Agric Food Chem;65(50):10884-10890, 2017 Dec 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cry11Aa displays high toxicity to the larvae of several mosquito species, including Aedes, Culex, and Anopheles. To study its binding characterization against Culex quinquefasciatus, Cry11Aa was purified and western blot results showed that Cry11Aa could bind successfully to the brush border membrane vesicles. To identify Cry11Aa-binding proteins in C. quinquefasciatus, a biotin-based protein pull-down experiment was performed and seven Cry11Aa-binding proteins were isolated from the midgut of C. quinquefasciatus larvae. Analysis of liquid chromatography-tandem mass spectrometry showed that one of the Cry11Aa-binding proteins is the ATP-binding domain 1 family member B. To investigate its binding property and effect on the toxicity of Cry11Aa, western blot, far-western blot, enzyme-linked immunosorbent assay, and bioassays of Cry11Aa in the presence and absence of the recombinant ATP-binding protein were performed. Our results showed that the ATP-binding protein interacted with Cry11Aa and increased the toxicity of Cry11Aa against C. quinquefasciatus. Our study suggests that midgut proteins other than the toxin receptors may modulate the toxicity of Cry toxins against mosquitoes.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/metabolismo
Proteínas de Bactérias/metabolismo
Culex/metabolismo
Endotoxinas/metabolismo
Proteínas Hemolisinas/metabolismo
Proteínas de Insetos/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/toxicidade
Culex/química
Culex/efeitos dos fármacos
Culex/genética
Endotoxinas/toxicidade
Proteínas Hemolisinas/toxicidade
Proteínas de Insetos/química
Proteínas de Insetos/genética
Larva/efeitos dos fármacos
Larva/genética
Larva/crescimento & desenvolvimento
Larva/metabolismo
Ligação Proteica
Domínios Proteicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Endotoxins); 0 (Hemolysin Proteins); 0 (Insect Proteins); 0 (insecticidal crystal protein, Bacillus Thuringiensis); 8L70Q75FXE (Adenosine Triphosphate)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04427


  8 / 6526 MEDLINE  
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[PMID]:29216317
[Au] Autor:Fraser JE; De Bruyne JT; Iturbe-Ormaetxe I; Stepnell J; Burns RL; Flores HA; O'Neill SL
[Ad] Endereço:Institute of Vector-Borne Disease, Monash University, Clayton, VIC, Australia.
[Ti] Título:Novel Wolbachia-transinfected Aedes aegypti mosquitoes possess diverse fitness and vector competence phenotypes.
[So] Source:PLoS Pathog;13(12):e1006751, 2017 Dec.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wolbachia pipientis from Drosophila melanogaster (wMel) is an endosymbiotic bacterium that restricts transmission of human pathogenic flaviviruses and alphaviruses, including dengue, Zika, and chikungunya viruses, when introduced into the mosquito vector Aedes aegypti. To date, wMel-infected Ae. aegypti have been released in field trials in 5 countries to evaluate the effectiveness of this strategy for disease control. Despite the success in establishing wMel-infected mosquitoes in wild populations, and the well-characterized antiviral capabilities of wMel, transinfecting different or additional Wolbachia strains into Ae. aegypti may improve disease impact, and perhaps more importantly, could provide a strategy to account for the possible evolution of resistant arboviruses. Here, we report the successful transinfection of Ae. aegypti with the Wolbachia strains wMelCS (D. melanogaster), wRi (D. simulans) and wPip (Culex quinquefasciatus) and assess the effects on Ae. aegypti fitness, cytoplasmic incompatibility, tissue tropism and pathogen blocking in a laboratory setting. The results demonstrate that wMelCS provides a similar degree of protection against dengue virus as wMel following an infectious blood meal, and significantly reduces viral RNA levels beyond that of wMel following a direct challenge with infectious virus in mosquitoes, with no additional fitness cost to the host. The protection provided by wRi is markedly weaker than that of wMelCS, consistent with previous characterisations of these lines in Drosophila, while wPip was found to substantially reduce the fitness of Ae. aegypti. Thus, we determine wMelCS as a key candidate for further testing in field-relevant fitness tests and viremic blood feeding challenges in a clinical setting to determine if it may represent an alternative Wolbachia strain with more desirable attributes than wMel for future field testing.
[Mh] Termos MeSH primário: Aedes/microbiologia
Transmissão Vertical de Doença Infecciosa/veterinária
Mosquitos Vetores/microbiologia
Wolbachia/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Aedes/crescimento & desenvolvimento
Aedes/fisiologia
Aedes/virologia
Animais
Controle de Doenças Transmissíveis/métodos
Culex/microbiologia
Vírus da Dengue/isolamento & purificação
Vírus da Dengue/fisiologia
Drosophila melanogaster/microbiologia
Drosophila simulans/microbiologia
Feminino
Fertilidade
Masculino
Controle de Mosquitos/métodos
Mosquitos Vetores/fisiologia
Mosquitos Vetores/virologia
Especificidade de Órgãos
Ovário/microbiologia
Ovário/fisiologia
RNA Viral/isolamento & purificação
Glândulas Salivares/microbiologia
Glândulas Salivares/fisiologia
Caracteres Sexuais
Especificidade da Espécie
Análise de Sobrevida
Tropismo Viral
Wolbachia/isolamento & purificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006751


  9 / 6526 MEDLINE  
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[PMID]:29252986
[Au] Autor:Ngufor C; Fongnikin A; Rowland M; N'Guessan R
[Ad] Endereço:London School of Hygiene and Tropical Medicine (LSHTM), London, United Kingdom.
[Ti] Título:Indoor residual spraying with a mixture of clothianidin (a neonicotinoid insecticide) and deltamethrin provides improved control and long residual activity against pyrethroid resistant Anopheles gambiae sl in Southern Benin.
[So] Source:PLoS One;12(12):e0189575, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: There is an urgent need for new insecticides for indoor residual spraying (IRS) which can provide improved and prolonged control of malaria vectors that have developed resistance to existing insecticides. The neonicotinoid, clothianidin represents a class of chemistry new to public health. Clothianidin acts as an agonist on nicotinic acetyl choline receptors. IRS with a mixture of Clothianidin and another WHO approved insecticide such as deltamethrin could provide improved control of insecticide resistant malaria vector populations and serve as a tool for insecticide resistance management. METHODS: The efficacy and residual activity of a novel IRS mixture of deltamethrin and clothianidin was evaluated against wild pyrethroid resistant An. gambiae sl in experimental huts in Cove, Benin. Two application rates of the mixture were tested and comparison was made with clothianidin and deltamethrin applied alone. To assess the residual efficacy of the treatments on different local wall substrates, the inner walls of the experimental huts were covered with either cement, mud or plywood. RESULTS: Clothianidin demonstrated a clear delayed expression in mortality of wild pyrethroid resistant An. gambiae sl in the experimental huts which reached its full effect 120 hours after exposure. Overall mortality over the 12-month hut trial was 15% in the control hut and 24-29% in the deltamethrin-treated huts. The mixture of clothianidin 200mg/m2 and deltamethrin 25mg/m2 induced high overall hut mortality rates (87% on mud walls, 82% on cement walls and 61% on wooden walls) largely due to the clothianidin component and high hut exiting rates (67-76%) mostly due to the deltamethrin component. Mortality rates remained >80% for 8-9 months on mud and cement walls. The residual activity trend was confirmed by results from monthly in situ cone bioassays with laboratory susceptible An. gambiae Kisumu strain. CONCLUSION: IRS campaigns with the mixture of clothianidin plus deltamethrin have the potential to provide prolonged control of malaria transmitted by pyrethroid resistant mosquito populations.
[Mh] Termos MeSH primário: Anopheles
Culex
Guanidinas
Resistência a Inseticidas
Inseticidas
Controle de Mosquitos/métodos
Neonicotinoides
Piretrinas
Tiazóis
[Mh] Termos MeSH secundário: Animais
Benin
Habitação
Seres Humanos
Malária/prevenção & controle
Organização Mundial da Saúde
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Guanidines); 0 (Insecticides); 0 (Neonicotinoids); 0 (Pyrethrins); 0 (Thiazoles); 2V9906ABKQ (clothianidin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189575


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[PMID]:27773635
[Au] Autor:Yang L; Piermarini PM
[Ad] Endereço:Department of Entomology, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, United States.
[Ti] Título:Molecular expression of aquaporin mRNAs in the northern house mosquito, Culex pipiens.
[So] Source:J Insect Physiol;96:35-44, 2017 01.
[Is] ISSN:1879-1611
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aquaporins (AQPs) are a family of membrane-bound proteins, originally described as water-channels, that broadly exist in prokaryotic and eukaryotic cells. However, some AQPs can transport small molecules (e.g. urea, glycerol) along with or preferentially to water. Previous work in the mosquitoes Aedes aegypti and Anopheles gambiae have characterized the molecular expression of one or more AQP genes and shown that they are involved in water homeostasis after blood feeding, tolerance to dehydration and heat stresses, and development of Plasmodium falciparum in the mosquito. However, the molecular expression of AQPs in the northern house mosquito Culex pipiens, the primary vector of West Nile virus, is presently unknown. Here we characterize the relative mRNA expression of six AQP genes in C. pipiens. We used quantitative RT-PCR (qPCR) to compare AQP mRNA levels 1) among different life stages and sexes, 2) between blood fed and non-blood fed adult females, 3) between adult females injected with phosphate-buffered saline (PBS) and non-injected females, and 4) between adult females exposed to a low vs. high relative humidity. We found that: 1) four AQP mRNAs (Drip, Bib, Eglp1, Aqp12L) were differentially expressed between larval and adult life stages, but not between sexes; 2) after a blood meal, the expression of two AQP mRNAs (Drip and Aqp12L) were down-regulated and one AQP mRNA (Eglp2) was up-regulated; 3) the expression of two AQP mRNAs (Drip and Prip) were down-regulated and one AQP mRNA (Eglp1) was up regulated after injecting the hemolymph of mosquitoes with PBS; and 4) the expression of Prip and Eglp2 mRNAs were both down-regulated in response to a low relative humidity. Our study is the first to characterize the molecular expression patterns of AQPs in C. pipiens, thus providing a foundation for future research elucidating the physiological and functional roles of AQPs in this important disease vector.
[Mh] Termos MeSH primário: Aquaporinas/genética
Culex/fisiologia
Expressão Gênica
Proteínas de Insetos/genética
[Mh] Termos MeSH secundário: Animais
Aquaporinas/metabolismo
Clonagem Molecular
Culex/genética
DNA Complementar/genética
DNA Complementar/metabolismo
Comportamento Alimentar
Hemolinfa/metabolismo
Proteínas de Insetos/metabolismo
Masculino
Filogenia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aquaporins); 0 (DNA, Complementary); 0 (Insect Proteins); 0 (RNA, Messenger)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171206
[Lr] Data última revisão:
171206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE



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