Base de dados : MEDLINE
Pesquisa : B01.050.500.131.617.720.500.500.875 [Categoria DeCS]
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  1 / 2718 MEDLINE  
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[PMID]:29236697
[Au] Autor:Sigut M; Kostovcík M; Sigutová H; Hulcr J; Drozd P; Hrcek J
[Ad] Endereço:Department of Biology and Ecology/Institute of Environmental Technologies, University of Ostrava, Ostrava, Czech Republic.
[Ti] Título:Performance of DNA metabarcoding, standard barcoding, and morphological approach in the identification of host-parasitoid interactions.
[So] Source:PLoS One;12(12):e0187803, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding interactions between herbivores and parasitoids is essential for successful biodiversity protection and monitoring and for biological pest control. Morphological identifications employ insect rearing and are complicated by insects' high diversity and crypsis. DNA barcoding has been successfully used in studies of host-parasitoid interactions as it can substantially increase the recovered real host-parasitoid diversity distorted by overlooked species complexes, or by species with slight morphological differences. However, this approach does not allow the simultaneous detection and identification of host(s) and parasitoid(s). Recently, high-throughput sequencing has shown high potential for surveying ecological communities and trophic interactions. Using mock samples comprising insect larvae and their parasitoids, we tested the potential of DNA metabarcoding for identifying individuals involved in host-parasitoid interactions to different taxonomic levels, and compared it to standard DNA barcoding and morphological approaches. For DNA metabarcoding, we targeted the standard barcoding marker cytochrome oxidase subunit I using highly degenerate primers, 2*300 bp sequencing on a MiSeq platform, and RTAX classification using paired-end reads. Additionally, using a large host-parasitoid dataset from a Central European floodplain forest, we assess the completeness and usability of a local reference library by confronting the number of Barcoding Index Numbers obtained by standard barcoding with the number of morphotypes. Overall, metabarcoding recovery was high, identifying 92.8% of the taxa present in mock samples, and identification success within individual taxonomic levels did not significantly differ among metabarcoding, standard barcoding, and morphology. Based on the current local reference library, 39.4% parasitoid and 90.7% host taxa were identified to the species level. DNA barcoding estimated higher parasitoid diversity than morphotyping, especially in groups with high level of crypsis. This study suggests the potential of metabarcoding for effectively recovering host-parasitoid diversity, together with more accurate identifications obtained from building reliable and comprehensive reference libraries, especially for parasitoids.
[Mh] Termos MeSH primário: Código de Barras de DNA Taxonômico
Interações Hospedeiro-Parasita
Himenópteros/crescimento & desenvolvimento
Larva
Lepidópteros/crescimento & desenvolvimento
Plantas/parasitologia
[Mh] Termos MeSH secundário: Animais
Herbivoria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187803


  2 / 2718 MEDLINE  
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[PMID]:29190301
[Au] Autor:Gao XK; Zhang S; Luo JY; Wang CY; Lü LM; Zhang LJ; Zhu XZ; Wang L; Cui JJ
[Ad] Endereço:State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, China.
[Ti] Título:Identification and validation of reference genes for gene expression analysis in Aphidius gifuensis (Hymenoptera: Aphidiidae).
[So] Source:PLoS One;12(11):e0188477, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reference genes have been utilized in estimating gene expression levels using quantitative reverse transcriptase-quantitative polymerase chain reaction (qRT-PCR) analysis. Aphidius gifuensis Ashmaed is one of the most widely used biological control agents for aphids. The biological properties of this species have been studied in detail, and current investigations are focused on elucidating the regulatory mechanisms in its host However, the appropriate reference genes for target gene expression studies have not been identified. In this study, the expression profiles of 12 candidate reference genes were evaluated under different experimental conditions(development stage, sex, tissue type, diet) by using dedicated algorithms, including geNorm, Normfinder, BestKeeper, and ΔCt. In addition, RefFinder was used to rank the overall stability of the candidate genes. Finally, we recommend three optimal reference genes for the normalization of qRT-PCR data in the presence of specific variables, which include ACTB, RPL13, and PPI for different developmental stages; RPS18, ACTB, and RPL13 for sexes; RPL13, PRII3, and RPS18 in different tissue types; and RPL13, RPL27, and ACTB in diverse diets. The present study has identified optimal reference genes that could be used in estimating the expression levels of specific genes under these conditions following the Minimum Information for publication of Quantitative real-time PCR Experiments (MIQE) guidelines, which would facilitate in advancements in functional genomics research on A. gifuensis.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica
Himenópteros/genética
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188477


  3 / 2718 MEDLINE  
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[PMID]:27773636
[Au] Autor:Boevé JL; Trenczek TE; Angeli S
[Ad] Endereço:OD Taxonomy and Phylogeny, Royal Belgian Institute of Natural Sciences, Rue Vautier 29, 1000 Brussels, Belgium. Electronic address: jean-luc.boeve@naturalsciences.be.
[Ti] Título:Searching for particular traits of sawfly (Hymenoptera: Tenthredinidae) larvae that emit hemolymph as a defence against predators.
[So] Source:J Insect Physiol;96:93-97, 2017 01.
[Is] ISSN:1879-1611
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Easy bleeding is a defence strategy that allows the larvae of some Tenthredinidae sawfly species to emit deterrent hemolymph when attacked by a predator. However, a drawback of this defence is that hemolymph is frequently in contact with the exterior, thus potentially subjected to multiple microbial infections at any body's integumental spot. Here we aimed to identify physiological traits that are linked to easy bleeding. First, larvae of several sawfly species were subjected to daily experimental losses of hemolymph equivalent to 10% of their body weight, and changes in body weight and survival were recorded. Easy bleeders' survival rates were better compared to non-easy bleeders. Second, testing hemolymph melanisation revealed that nearly all sawfly hemolymph samples did not melanise over a 24h period. Third, inhibition zone tests against live Escherichia coli were conducted using hemolymph collected 24-48h after a sterile wounding and an infection with Micrococcus luteus, as well as from control, untouched individuals. Sterile wounding induced similar antibacterial activities compared to those detected in the control group. However, the activity was significantly enhanced upon infection in some species, similarly to other insects. Thus, easy bleeders have a tendency to compensate for hemolymph loss resulting from predator-prey interactions, whereas a non-melanising hemolymph is probably a characteristic of sawflies, and the antimicrobial activity can be high but is comparable in easy bleeders versus other insects.
[Mh] Termos MeSH primário: Himenópteros/fisiologia
Imunidade Inata
[Mh] Termos MeSH secundário: Animais
Cadeia Alimentar
Hemolinfa/metabolismo
Himenópteros/crescimento & desenvolvimento
Himenópteros/microbiologia
Larva/crescimento & desenvolvimento
Larva/microbiologia
Larva/fisiologia
Comportamento Predatório
Distribuição Aleatória
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171206
[Lr] Data última revisão:
171206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  4 / 2718 MEDLINE  
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[PMID]:28950273
[Au] Autor:Rosman Y; Confino-Cohen R; Goldberg A
[Ad] Endereço:Allergy and Clinical Immunology Unit, Meir Medical Center, Kfar Saba, Israel.
[Ti] Título:Venom Immunotherapy in High-Risk Patients: The Advantage of the Rush Build-Up Protocol.
[So] Source:Int Arch Allergy Immunol;174(1):45-51, 2017.
[Is] ISSN:1423-0097
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Venom immunotherapy (VIT) is considered to be the gold standard treatment for patients with hymenoptera venom allergy. This treatment induces systemic reactions (SR) in a significant number of patients. OBJECTIVE: To evaluate the outcome of VIT in patients with known risk factors for VIT-induced SR and to compare rush VIT (RVIT) and conventional VIT (CVIT). METHODS: All of the patients who received VIT and had at least one of the following risk factors were included: current cardiovascular disease, uncontrolled asthma, high basal serum tryptase, current treatment with ß-blockers or angiotensin-converting enzyme inhibitors, and age >70 or <5 years. RESULTS: Sixty-four patients were included, and most of them (52; 81.5%) were allergic exclusively to bee venom. Thirty-five (54.7%) patients underwent RVIT and 29 CVIT. The incidence of patients who developed SR during the build-up phase was similar for RVIT and CVIT (25.7 and 27.5%, respectively; p = 1). However, the incidence of SR per injection was significantly higher in CVIT than in RVIT (5.6 and 2.75%, respectively; p = 0.01). Most reactions (79.1%) were mild, limited to the skin. Most of the patients (92.1%) reached the full maintenance dose of 100 µg. This dose was reached by a significantly larger number of patients receiving RVIT compared to CVIT (100 and 82.7%, respectively; p = 0.01). None of the patients experienced exacerbation of their concurrent chronic disease during VIT. CONCLUSION: VIT can be performed safely and efficiently in patients with risk factors for immunotherapy. In these patients RVIT appears to be safer and more efficient than CVIT.
[Mh] Termos MeSH primário: Alérgenos/administração & dosagem
Venenos de Abelha/administração & dosagem
Dessensibilização Imunológica/métodos
Venenos de Vespas/administração & dosagem
[Mh] Termos MeSH secundário: Adulto
Idoso
Animais
Venenos de Abelha/imunologia
Pré-Escolar
Feminino
Seres Humanos
Himenópteros/imunologia
Masculino
Estudos Retrospectivos
Venenos de Vespas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Bee Venoms); 0 (Wasp Venoms)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1159/000479692


  5 / 2718 MEDLINE  
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[PMID]:28898258
[Au] Autor:Bonamigo T; Campos JF; Oliveira AS; Torquato HFV; Balestieri JBP; Cardoso CAL; Paredes-Gamero EJ; de Picoli Souza K; Dos Santos EL
[Ad] Endereço:Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados, MS, Brazil.
[Ti] Título:Antioxidant and cytotoxic activity of propolis of Plebeia droryana and Apis mellifera (Hymenoptera, Apidae) from the Brazilian Cerrado biome.
[So] Source:PLoS One;12(9):e0183983, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Propolis is a complex bioactive mixture produced by bees, known to have different biological activities, especially in countries where there is a rich biodiversity of plant species. The objective of this study was to determine the chemical composition and evaluate the antioxidant and cytotoxic properties of Brazilian propolis from the species Plebeia droryana and Apis mellifera found in Mato Grosso do Sul, Brazil. In the ethanolic extracts of P. droryana propolis (ExEP-P) and A. mellifera (ExEP-A) acids, phenolic compounds, terpenes and tocopherol were identified as major compounds. Both extracts presented antioxidant activity against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, the maximum activities being 500 µg/mL (ExEP-P) and 300 µg/mL (ExEP-A). However, only ExEP-A was able to inhibit lipid peroxidation induced by the oxidizing agent 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), which inhibited oxidative hemolysis and reduced the levels of malondialdehyde (MDA) in human erythrocytes for 4 h of incubation. The extracts also reduced the cell viability of the K562 erythroleukemia tumour line, with a predominance of necrotic death. Thus, it is concluded that the propolis produced by P. droryana and A. mellifera contain important compounds capable of minimizing the action of oxidizing substances in the organism and reducing the viability of erythroleukemia cells.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Himenópteros/química
Própole/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/química
Antioxidantes/toxicidade
Brasil
Caspase 3/metabolismo
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Fracionamento Químico
Cromatografia Líquida de Alta Pressão
Eritrócitos/efeitos dos fármacos
Eritrócitos/metabolismo
Radicais Livres/antagonistas & inibidores
Cromatografia Gasosa-Espectrometria de Massas
Hemólise/efeitos dos fármacos
Seres Humanos
Células K562
Peroxidação de Lipídeos/efeitos dos fármacos
Malondialdeído/metabolismo
Oxirredução/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Própole/química
Própole/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Free Radicals); 4Y8F71G49Q (Malondialdehyde); 9009-62-5 (Propolis); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183983


  6 / 2718 MEDLINE  
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[PMID]:28881958
[Au] Autor:Souza MF; Veloso LFA; Sampaio MV; Davis JA
[Ad] Endereço:Department of Entomology, Louisiana State University, 404 Life Sciences, Baton Rouge, LA 70803.
[Ti] Título:Influence of Host Quality and Temperature on the Biology of Diaeretiella rapae (Hymenoptera: Braconidae, Aphidiinae).
[So] Source:Environ Entomol;46(4):995-1004, 2017 08 01.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Biological features of Diaeretiella rapae (McIntosh), an aphid parasitoid, are conditioned by temperature and host. However, studies of host quality changes due to temperature adaptability have not been performed previously. Therefore, this study evaluated the adaptability of Lipaphis pseudobrassicae (Davis) and Myzus persicae (Sulzer) to high temperature, high temperature effect on their quality as hosts for D. rapae, and on parasitoid's thermal threshold. Aphid development, survivorship, fecundity, and longevity were compared at 19 °C and 28 °C. Host quality in different temperatures was determined through evaluation of parasitoid biology. Thermal threshold of D. rapae was determined using development time data. At 28 °C, development time, rate of immature survival, and total fecundity rates were greater in L. pseudobrassicae than in M. persicae. Development time of D. rapae in L. pseudobrassicae was shorter than that in M. persicae at 28 °C and 31 °C for females and at 31 °C for males. The thermal threshold of D. rapae was 6.38 °C and 3.33 °C for females and 4.45 °C and 3.63 °C for males developed on L. pseudobrassicae and M. persicae, respectively. Diaeretiella rapae size gain was greater in L. pseudobrassicae than that in M. persicae at 25 °C and 28 °C. Lipaphis pseudobrassicae showed better adaptation than M. persicae to elevated temperatures, which resulted in a better quality host for D. rapae at temperatures of 28 °C and 31 °C and a higher lower thermal threshold when the parasitoid developed within L. pseudobrassicae. The host's adaptation to high temperatures is a determinant of host quality for the parasitoid at that same climatic condition.
[Mh] Termos MeSH primário: Afídeos/fisiologia
Afídeos/parasitologia
Interações Hospedeiro-Parasita
Himenópteros/fisiologia
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Animais
Afídeos/crescimento & desenvolvimento
Feminino
Himenópteros/crescimento & desenvolvimento
Larva/crescimento & desenvolvimento
Larva/parasitologia
Larva/fisiologia
Masculino
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvx108


  7 / 2718 MEDLINE  
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[PMID]:28854232
[Au] Autor:Shrestha G; Skovgård H; Reddy GVP; Steenberg T; Enkegaard A
[Ad] Endereço:Western Triangle Ag Research Center, Montana State University, Conrad, MT, United States of America.
[Ti] Título:Role of the aphid species and their feeding locations in parasitization behavior of Aphelinus abdominalis, a parasitoid of the lettuce aphid Nasonovia ribisnigri.
[So] Source:PLoS One;12(8):e0184080, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aphid species feeding on lettuce occupy distinct feeding sites: the lettuce aphid Nasonovia ribisnigri prefers to feed on heart leaves, whereas the potato aphid Macrosiphum euphorbiae feeds only on outer leaves. The aphid parasitoid Aphelinus abdominalis, known to be able to regulate M. euphorbiae on many crops, has recently been indicated as a promising biocontrol candidate also for use against N. ribisnigri, a major pest of lettuce. This study therefore examined A. abdominalis parasitization preference between N. ribisnigri and M. euphorbiae and its ability to parasitize aphids feeding on different parts of lettuce plants. In addition, life history traits of A. abdominalis on these aphid species were investigated. In no-choice laboratory experiments on leaf discs and 24 h exposure, A. abdominalis successfully parasitized 54% and 60% of the offered N. ribisnigri and M. euphorbiae, respectively, with no significant difference. In the corresponding choice experiment, however, A. abdominalis had a tendency for a significantly higher preference for M. euphorbiae (38%) compared to N. ribisnigri (30%). Growth chamber experiments on whole plants demonstrated that A. abdominalis was able to parasitize aphids, regardless of their feeding locations on lettuce plants. However, aphid feeding behavior had a significant effect on the parasitization rate. A. abdominalis parasitized significantly higher percentages of M. euphorbiae or N. ribisnigri when aphids were exposed separately to parasitoids on whole lettuce plants as compared with N. ribisnigri exposed only on heart leaf. A significant preference of A. abdominalis for M. euphorbiae compared to N. ribisnigri was also observed in the growth chamber choice experiment. A high percentage of adult emergence (> 84%) and female-biased sex ratio (> 83%) were found irrespective of the aphid species.
[Mh] Termos MeSH primário: Afídeos/parasitologia
Himenópteros/fisiologia
Alface/parasitologia
Controle Biológico de Vetores/métodos
[Mh] Termos MeSH secundário: Animais
Afídeos/fisiologia
Feminino
Himenópteros/crescimento & desenvolvimento
Masculino
Doenças das Plantas/parasitologia
Doenças das Plantas/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184080


  8 / 2718 MEDLINE  
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[PMID]:28785793
[Au] Autor:Kubo R; Harano KI; Ono M
[Ad] Endereço:Honeybee Science Research Center, Tamagawa University, 6-1-1, Tamagawagakuen, Machida, Tokyo, 194-8610, Japan. kubo@agr.tamagawa.ac.jp.
[Ti] Título:Male scent-marking pheromone of Bombus ardens ardens (Hymenoptera; Apidae) attracts both conspecific queens and males.
[So] Source:Naturwissenschaften;104(9-10):69, 2017 Oct.
[Is] ISSN:1432-1904
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:To explore the role of the volatiles emitted from male labial gland (LG) of the bumblebee Bombus ardens ardens, we investigated the responses of virgin queens and males to volatiles using a gas chromatography-electroantennographic detector (GC-EAD) system and Y-tube olfactometer. GC-EAD analysis revealed that citronellol, the main compound detected in the male LG, caused clear electrophysiological responses in the antennae of B. a. ardens virgin queens and males although two minor compounds elicited antennal responses when applied in a high concentration. Behavioral tests using a Y-tube olfactometer showed that queens and males were significantly attracted to both LG extracts and citronellol more than to the solvent alone. This is the first study to demonstrate that citronellol as a major compound of male scent-marking pheromone in B. a. ardens functions as a sex attractant for queens. The results also suggest that this compound has another function as a trail marker used by males.
[Mh] Termos MeSH primário: Abelhas
[Mh] Termos MeSH secundário: Animais
Cromatografia Gasosa
Feminino
Himenópteros
Masculino
Feromônios
Atrativos Sexuais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pheromones); 0 (Sex Attractants)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE
[do] DOI:10.1007/s00114-017-1493-1


  9 / 2718 MEDLINE  
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[PMID]:28732030
[Au] Autor:Peng Y; Wang SN; Li KM; Liu JT; Zheng Y; Shan S; Yang YQ; Li RJ; Zhang YJ; Guo YY
[Ad] Endereço:College of Plant Protection, Agricultural University of Hebei, Baoding, China.
[Ti] Título:Identification of odorant binding proteins and chemosensory proteins in Microplitis mediator as well as functional characterization of chemosensory protein 3.
[So] Source:PLoS One;12(7):e0180775, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play important roles in transporting semiochemicals through the sensillar lymph to olfactory receptors in insect antennae. In the present study, twenty OBPs and three CSPs were identified from the antennal transcriptome of Microplitis mediator. Ten OBPs (MmedOBP11-20) and two CSPs (MmedCSP2-3) were newly identified. The expression patterns of these new genes in olfactory and non-olfactory tissues were investigated by real-time quantitative PCR (qPCR) measurement. The results indicated that MmedOBP14, MmedOBP18, MmedCSP2 and MmedCSP3 were primarily expressed in antennae suggesting potential olfactory roles in M. mediator. However, other genes including MmedOBP11-13, 15-17, 19-20 appeared to be expressed at higher levels in body parts than in antennae. Focusing on the functional characterization of MmedCSP3, immunocytochemistry and fluorescent competitive binding assays were conducted indoors. It was found that MmedCSP3 was specifically located in the sensillum lymph of olfactory sensilla basiconca type 2. The recombinant MmedCSP3 could bind several types of host insects odors and plant volatiles. Interestingly, three sex pheromone components of Noctuidae insects, cis-11-hexadecenyl aldehyde (Z11-16: Ald), cis-11-hexadecanol (Z11-16: OH), and trans-11-tetradecenyl acetate (E11-14: Ac), showed high binding affinities (Ki = 17.24-18.77 µM). The MmedCSP3 may be involved in locating host insects. Our data provide a base for further investigating the physiological roles of OBPs and CSPs in M. mediator, and extend the function of MmedCSP3 in chemoreception of M. mediator.
[Mh] Termos MeSH primário: Himenópteros/metabolismo
Proteínas de Insetos/metabolismo
Receptores Odorantes/metabolismo
[Mh] Termos MeSH secundário: Animais
Antenas de Artrópodes/citologia
Antenas de Artrópodes/metabolismo
Western Blotting
Feminino
Expressão Gênica
Perfilação da Expressão Gênica
Himenópteros/citologia
Imuno-Histoquímica
Masculino
Filogenia
Reação em Cadeia da Polimerase
Ligação Proteica
Proteínas Recombinantes/metabolismo
Homologia de Sequência de Aminoácidos
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Receptors, Odorant); 0 (Recombinant Proteins); 0 (odorant-binding protein)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180775


  10 / 2718 MEDLINE  
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[PMID]:28686887
[Au] Autor:Friol PS; Catae AF; Tavares DA; Malaspina O; Roat TC
[Ad] Endereço:UNESP - Univ. Estadual Paulista, campus de Rio Claro, Instituto de Biociências, Departamento de Biologia, Centro de Estudos de Insetos Sociais, Rio Claro, SP, Brazil. Electronic address: priscilasfriol@yahoo.com.br.
[Ti] Título:Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees?
[So] Source:Chemosphere;185:56-66, 2017 Oct.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The use of insecticides on crops can affect non-target insects, such as bees. In addition to the adult bees, larvae can be exposed to the insecticide through contaminated floral resources. Therefore, this study aimed to investigate the possible effects of the exposure of A. mellifera larvae to a field concentration of thiamethoxam (0.001 ng/µL thiamethoxam) on larval and pupal survival and on the percentage of adult emergence. Additionally, its cytotoxic effects on the digestive cells of midgut, Malpighian tubules cells and Kenyon cells of the brain of newly emerged A. mellifera bees were analyzed. The results showed that larval exposure to this concentration of thiamethoxam did not influence larval and pupal survival or the percentage of adult bee emergence. However, this exposure caused ultra-structural alterations in the target and non-target organs of newly emerged bees. The digestive cell of bees that were exposed to the insecticide exhibited a basal labyrinth without long and thin channels and compromised mitochondria. In Malpighian tubules cells, disorganized basal labyrinth, dilated mitochondria with a deformed shape and a loss of cristae, and disorganized microvilli were observed. The results showed that the exposed bees presented Kenyon cells with alterations in the nucleus and mitochondria. These alterations indicate possible tissue degeneration, demonstrating the cytotoxicity of thiamethoxam in the target and non-target organs of newly emerged bees. Such results suggest cellular organelle impairment that can compromise cellular function of the midgut cells, Malpighian tubules cells and Kenyon cells, and, consequently, can compromise the longevity of the bees of the whole colony.
[Mh] Termos MeSH primário: Abelhas/fisiologia
Inseticidas/toxicidade
Nitrocompostos/toxicidade
Oxazinas/toxicidade
Tiazóis/toxicidade
[Mh] Termos MeSH secundário: Animais
Produtos Agrícolas
Sistema Digestório
Himenópteros
Larva/efeitos dos fármacos
Longevidade
Neonicotinoides
Pupa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 0 (Neonicotinoids); 0 (Nitro Compounds); 0 (Oxazines); 0 (Thiazoles); 747IC8B487 (thiamethoxam)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE



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