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Pesquisa : B01.050.500.131.617.720.500.500.937.200 [Categoria DeCS]
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  1 / 2770 MEDLINE  
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[PMID]:29385390
[Au] Autor:Chen T; Cong Q; Qi Y; Jin J; Choy KL
[Ad] Endereço:Key Laboratory of Bionic Engineering, Ministry of Education, Jilin University, Changchun, Jilin Province, P.R., China.
[Ti] Título:Hydrophobic durability characteristics of butterfly wing surface after freezing cycles towards the design of nature inspired anti-icing surfaces.
[So] Source:PLoS One;13(1):e0188775, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The hydrophobicity and anti-icing performance of the surfaces of some artificial hydrophobic coatings degraded after several icing and de-icing cycles. In this paper, the frost formation on the surfaces of butterfly wings from ten different species was observed, and the contact angles were measured after 0 to 6 frosting/defrosting cycles. The results show that no obvious changes in contact angle for the butterfly wing specimens were not obvious during the frosting/defrosting process. Further, the conclusion was inferred that the topography of the butterfly wing surface forms a special space structure which has a larger space inside that can accommodate more frozen droplets; this behavior prevents destruction of the structure. The findings of this study may provide a basis and new concepts for the design of novel industrially important surfaces to inhibit frost/ice growth, such as durable anti-icing coatings, which may decrease or prevent the socio-economic loss.
[Mh] Termos MeSH primário: Borboletas
Congelamento
Gelo
Asas de Animais
[Mh] Termos MeSH secundário: Animais
Borboletas/classificação
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ice)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188775


  2 / 2770 MEDLINE  
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[PMID]:28466477
[Au] Autor:Stålhandske S; Gotthard K; Leimar O
[Ad] Endereço:Department of Zoology, Stockholm University, 106 91, Stockholm, Sweden.
[Ti] Título:Winter chilling speeds spring development of temperate butterflies.
[So] Source:J Anim Ecol;86(4):718-729, 2017 07.
[Is] ISSN:1365-2656
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Understanding and predicting phenology has become more important with ongoing climate change and has brought about great research efforts in the recent decades. The majority of studies examining spring phenology of insects have focussed on the effects of spring temperatures alone. Here we use citizen-collected observation data to show that winter cold duration, in addition to spring temperature, can affect the spring emergence of butterflies. Using spatial mixed models, we disentangle the effects of climate variables and reveal impacts of both spring and winter conditions for five butterfly species that overwinter as pupae across the UK, with data from 1976 to 2013 and one butterfly species in Sweden, with data from 2001 to 2013. Warmer springs lead to earlier emergence in all species and milder winters lead to statistically significant delays in three of the five investigated species. We also find that the delaying effect of winter warmth has become more pronounced in the last decade, during which time winter durations have become shorter. For one of the studied species, Anthocharis cardamines (orange tip butterfly), we also make use of parameters determined from previous experiments on pupal development to model the spring phenology. Using daily temperatures in the UK and Sweden, we show that recent variation in spring temperature corresponds to 10-15 day changes in emergence time over UK and Sweden, whereas variation in winter duration corresponds to 20 days variation in the south of the UK versus only 3 days in the south of Sweden. In summary, we show that short winters delay phenology. The effect is most prominent in areas with particularly mild winters, emphasising the importance of winter for the response of ectothermic animals to climate change. With climate change, these effects may become even stronger and apply also at higher latitudes.
[Mh] Termos MeSH primário: Borboletas/crescimento & desenvolvimento
Mudança Climática
Temperatura Ambiente
[Mh] Termos MeSH secundário: Animais
Estações do Ano
Suécia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1111/1365-2656.12673


  3 / 2770 MEDLINE  
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[PMID]:28455184
[Au] Autor:Schweizer F; Heidel-Fischer H; Vogel H; Reymond P
[Ad] Endereço:Department of Plant Molecular Biology, University of Lausanne, 1015 Lausanne, Switzerland.
[Ti] Título:Arabidopsis glucosinolates trigger a contrasting transcriptomic response in a generalist and a specialist herbivore.
[So] Source:Insect Biochem Mol Biol;85:21-31, 2017 06.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytophagous insects have to deal with toxic defense compounds from their host plants. Although it is known that insects have evolved genes and mechanisms to detoxify plant allochemicals, how specialist and generalist precisely respond to specific secondary metabolites at the molecular level is less understood. Here we studied the larval performance and transcriptome of the generalist moth Heliothis virescens and the specialist butterfly Pieris brassicae feeding on Arabidopsis thaliana genotypes with different glucosinolate (GS) levels. H. virescens larvae gained significantly more weight on the GS-deficient mutant quadGS compared to wild-type (Col-0) plants. On the contrary, P. brassicae was unaffected by the presence of GS and performed equally well on both genotypes. Strikingly, there was a considerable differential gene expression in H. virescens larvae feeding on Col-0 compared to quadGS. In contrast, compared to H. virescens, P. brassicae displayed a much-reduced transcriptional activation when fed on both plant genotypes. Transcripts coding for putative detoxification enzymes were significantly upregulated in H. virescens, along with digestive enzymes and transposable elements. These data provide an unprecedented view on transcriptional changes that are specifically activated by GS and illustrate differential molecular responses that are linked to adaptation to diet in lepidopteran herbivores.
[Mh] Termos MeSH primário: Borboletas/efeitos dos fármacos
Glucosinolatos/farmacologia
Herbivoria
Mariposas/efeitos dos fármacos
Transcriptoma/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Arabidopsis
Borboletas/genética
Borboletas/metabolismo
Regulação para Baixo
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Inativação Metabólica/genética
Proteínas de Insetos/metabolismo
Larva/efeitos dos fármacos
Mariposas/genética
Mariposas/metabolismo
Análise de Sequência de RNA
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glucosinolates); 0 (Insect Proteins); 0 (cuticle proteins, insects); 0 (storage proteins, Insecta)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  4 / 2770 MEDLINE  
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[PMID]:29193258
[Au] Autor:Xiang M; Zhang X; Deng Y; Li Y; Yu J; Zhu J; Huang X; Zhou J; Liao H
[Ad] Endereço:School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, China.
[Ti] Título:Comparative transcriptome analysis provides insights of anti-insect molecular mechanism of Cassia obtusifolia trypsin inhibitor against Pieris rapae.
[So] Source:Arch Insect Biochem Physiol;97(1), 2018 Jan.
[Is] ISSN:1520-6327
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pieris rapae, a serious Lepidoptera pest of cultivated crucifers, utilizes midgut enzymes to digest food and detoxify secondary metabolites from host plants. A recombinant trypsin inhibitor (COTI) from nonhost plant, Cassia obtusifolia, significantly decreased activities of trypsin-like proteases in the larval midgut on Pieris rapae and could suppress the growth of larvae. In order to know how COTI took effect, transcriptional profiles of P. rapae midgut in response to COTI was studied. A total of 51,544 unigenes were generated and 45.86% of which had homologs in public databases. Most of the regulated genes associated with digestion, detoxification, homeostasis, and resistance were downregulated after ingestion of COTI. Meanwhile, several unigenes in the integrin signaling pathway might be involved in response to COTI. Furthermore, using comparative transcriptome analysis, we detected differently expressing genes and identified a new reference gene, UPF3, by qRT-polymerase chain reaction (PCR). Therefore, it was suggested that not only proteolysis inhibition, but also suppression of expression of genes involved in metabolism, development, signaling, and defense might account for the anti-insect resistance of COTI.
[Mh] Termos MeSH primário: Borboletas/enzimologia
Cassia/metabolismo
Proteínas de Plantas/metabolismo
Transcriptoma
Inibidores da Tripsina/metabolismo
[Mh] Termos MeSH secundário: Animais
Borboletas/crescimento & desenvolvimento
Trato Gastrointestinal/enzimologia
Perfilação da Expressão Gênica
Larva/enzimologia
Larva/crescimento & desenvolvimento
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Recombinant Proteins); 0 (Trypsin Inhibitors)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1002/arch.21427


  5 / 2770 MEDLINE  
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[PMID]:29232405
[Au] Autor:Phon CK; Kirton LG; Yusoff NR
[Ad] Endereço:Tropical Forest Biodiversity Centre, Forest Research Institute Malaysia (FRIM), Kepong, Selangor, Malaysia.
[Ti] Título:Monitoring butterflies using counts of puddling males: A case study of the Rajah Brooke's Birdwing (Trogonoptera brookiana albescens).
[So] Source:PLoS One;12(12):e0189450, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Rajah Brooke's Birdwing, Trogonoptera brookiana, is a large, iconic butterfly that is facing heavy commercial exploitation and habitat loss. Males of some subspecies exhibit puddling behavior. A method of conservation monitoring was developed for subspecies albescens in Ulu Geroh, Peninsular Malaysia, where the males consistently puddle in single-species aggregations at stable geothermal springs, reaching well over 300 individuals when the population is at its highest. Digital photography was used to conduct counts of numbers of males puddling. The numbers of birdwings puddling were significantly correlated with counts of birdwings in flight, but were much higher. The numbers puddling during the peak hour were correlated with numbers puddling throughout the day and could be predicted using the numbers puddling at an alternative hour, enabling flexibility in the time of counts. Average counts for three images taken at each puddle at three peak hours between 1400-1600 hours over 2-3 days were used as a monthly population index. The numbers puddling were positively associated with higher relative humidity and brightness during monitoring hours. Monthly counts of birdwings from monitoring of puddles over a period of two years are presented. The minimum effort required for a monitoring program using counts of puddling males is discussed, as well as the potential of using the method to monitor other species of puddling butterflies.
[Mh] Termos MeSH primário: Borboletas
[Mh] Termos MeSH secundário: Animais
Conservação dos Recursos Naturais
Malásia
Masculino
Densidade Demográfica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189450


  6 / 2770 MEDLINE  
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[PMID]:28457724
[Au] Autor:Hiroyoshi S; Yoshimura J; Iwabuchi K; Reddy GVP; Mitsuhashi J
[Ad] Endereço:Laboratory of Applied Entomology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. Electronic address: satoshi_hiroyoshi@yahoo.co.jp.
[Ti] Título:Effects of pre-overwintering conditions on eupyrene and apyrene spermatogenesis after overwintering in Polygonia c-aureum (Lepidoptera: Nymphalidae).
[So] Source:J Insect Physiol;100:1-8, 2017 07.
[Is] ISSN:1879-1611
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sperm polymorphism is widely known in invertebrates. In insects, Lepidoptera has two types of sperm: nucleated eupyrene (fertile) sperm and anucleated apyrene (unfertile) sperm. These sperm types are produced during post-embryogenesis, and eupyrene spermatogenesis precedes apyrene spermatogenesis. During overwintering, spermatogenesis stops and a portion of undifferentiated-stage spermatocytes degenerate. After overwintering, spermatogenesis restarts with unaffected spermatogonia. However, how new spermatozoa arise in the adult testes after overwintering is not known in Lepidoptera. In this study, we investigated the spermatogenesis events in the nymphalid butterfly Polygonia c-aureum after overwintering under various environmental conditions. Our results indicate that both eupyrene and apyrene spermatogenesis restart at any stopping stage and sperm of these types are regenerated in no particular order after adult insect overwintering. This suggests that the spermatogenesis occurring after overwintering proceeds without embryogenetic restrictions related to the developmental sequence.
[Mh] Termos MeSH primário: Borboletas/fisiologia
Espermatogênese
[Mh] Termos MeSH secundário: Animais
Masculino
Estações do Ano
Testículo/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171206
[Lr] Data última revisão:
171206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  7 / 2770 MEDLINE  
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[PMID]:28973485
[Au] Autor:Ng SY; Bhardwaj S; Monteiro A
[Ad] Endereço:Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543.
[Ti] Título:Males Become Choosier in Response to Manipulations of Female Wing Ornaments in Dry Season Bicyclus anynana Butterflies.
[So] Source:J Insect Sci;17(4), 2017 Jul 01.
[Is] ISSN:1536-2442
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Male investment towards reproduction is substantial in some species, and this leads to the evolution of choosy males. Male choice is often directed towards female phenotypes that are good indicators of fecundity such as body size, age, or virgin status, and often acts in the same direction as fecundity selection. In insects, only a few examples exist where male choice is directed towards female ornaments as proxies Butler of female quality. Here, we use dry season males of the sex-role reversed butterfly species Bicyclus anynana to test for differences in male choosiness and investment towards females of varying attractiveness using ornament-manipulations. Male reproductive investment in this species is in the form of a nuptial gift, a spermatophore, given to females upon mating. Males were placed in cages with either wild-type ornamented females or with nonornamented females (center of the dorsal forewing eyespots painted over), and time to mating, duration of mating, and longevity of males and females after a single mating were measured. Ornament manipulations consisted of blocking the UV-reflective scales in the center of the dorsal forewing eyespots of females, a known sexual ornament. Males displayed lower latency to mate and longer mating durations with ornamented females. The longer mating duration did not, however, translate in the transfer of a nuptial gift that increased female longevity or reduced male longevity. Instead, we propose that longer mating durations with ornamented females may represent increased mate guarding behavior or increased sperm transfer.
[Mh] Termos MeSH primário: Borboletas
Preferência de Acasalamento Animal
Pigmentação
[Mh] Termos MeSH secundário: Animais
Copulação
Feminino
Longevidade
Masculino
Reprodução
Asas de Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1093/jisesa/iex053


  8 / 2770 MEDLINE  
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[PMID]:28961914
[Au] Autor:Pocius VM; Debinski DM; Pleasants JM; Bidne KG; Hellmich RL; Brower LP
[Ad] Endereço:Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011.
[Ti] Título:Milkweed Matters: Monarch Butterfly (Lepidoptera: Nymphalidae) Survival and Development on Nine Midwestern Milkweed Species.
[So] Source:Environ Entomol;46(5):1098-1105, 2017 10 01.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The population of monarch butterflies east of the Rocky Mountains has experienced a significant decline over the past 20 yr. In order to increase monarch numbers in the breeding range, habitat restoration that includes planting milkweed plants is essential. Milkweeds in the genus Asclepias and Cynanchum are the only host plants for larval monarch butterflies in North America, but larval performance and survival across nine milkweeds native to the Midwest is not well documented. We examined development and survival of monarchs from first-instar larval stages to adulthood on nine milkweed species native to Iowa. The milkweeds included Asclepias exaltata (poke milkweed) (Gentianales: Apocynaceae), Asclepias hirtella (tall green milkweed) (Gentianales: Apocynaceae), Asclepias incarnata (swamp milkweed) (Gentianales: Apocynaceae), Asclepias speciosa (showy milkweed) (Gentianales: Apocynaceae), Asclepias sullivantii (prairie milkweed) (Gentianales: Apocynaceae), Asclepias syriaca (common milkweed) (Gentianales: Apocynaceae), Asclepias tuberosa (butterfly milkweed) (Gentianales: Apocynaceae), Asclepias verticillata (whorled milkweed) (Gentianales: Apocynaceae), and Cynanchum laeve (honey vine milkweed) (Gentianales: Apocynaceae). In greenhouse experiments, fewer larvae that fed on Asclepias hirtella and Asclepias sullivantii reached adulthood compared with larvae that fed on the other milkweed species. Monarch pupal width and adult dry mass differed among milkweeds, but larval duration (days), pupal duration (days), pupal mass, pupal length, and adult wet mass were not significantly different. Both the absolute and relative adult lipids were different among milkweed treatments; these differences are not fully explained by differences in adult dry mass. Monarch butterflies can survive on all nine milkweed species, but the expected survival probability varied from 30 to 75% among the nine milkweed species.
[Mh] Termos MeSH primário: Asclepias
Borboletas/crescimento & desenvolvimento
Herbivoria
[Mh] Termos MeSH secundário: Animais
Borboletas/química
Feminino
Larva/crescimento & desenvolvimento
Lipídeos/análise
Masculino
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Lipids)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170930
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvx137


  9 / 2770 MEDLINE  
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[PMID]:28881951
[Au] Autor:Iserhard CA; Romanowski HP; Richter A; Mendonça MS
[Ad] Endereço:Departamento de Ecologia, Zoologia e Genética, Universidade Federal de Pelotas, Programa de Pós-Graduação em Biologia Animal, Brazil.
[Ti] Título:Monitoring Temporal Variation to Assess Changes in the Structure of Subtropical Atlantic Forest Butterfly Communities.
[So] Source:Environ Entomol;46(4):804-813, 2017 08 01.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The study of fauna through long-term surveys is important in unveiling how temporal patterns shape the structure of communities in tropical habitats. The butterfly assemblage of the subtropical Atlantic Forest may be considered highly diverse and shows changes in diversity and composition over time, highlighting the importance of long-term inventories. This work assessed temporal diversity patterns in the distribution and composition of butterfly assemblages in an Atlantic Forest site in southern Brazil using combined data from three years of standardized sampling with entomological nets, increasing the knowledge on this group in the Neotropics for monitoring and conservation. The butterfly fauna was analyzed in terms of richness, abundance, and composition. The inventories reached 401 species, with 14,442 butterfly individuals sampled. All the diversity parameters evaluated show significant differences between the first year of sampling compared to the second and third years. The latter had higher values of richness and abundance, followed by the first and second years. Hesperiidae was the richest family, followed by Nymphalidae and Lycaenidae, indicating a good representation of the assemblage as a whole. The results of this work are important for developing conservation programs in the Atlantic Forest and other forested environments in the neotropics, especially concerning reliable diversity assessments for the monitoring and management of protected areas. Decision making and public policy might also benefit from knowledge on temporal patterns of diversity regarding the maintenance of native habitats and integrity of biomes and their associated fauna.
[Mh] Termos MeSH primário: Biodiversidade
Borboletas/fisiologia
Florestas
[Mh] Termos MeSH secundário: Animais
Brasil
Dinâmica Populacional
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvx115


  10 / 2770 MEDLINE  
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[PMID]:28765284
[Au] Autor:Oda T; Hirabayashi H; Shikauchi G; Takamura R; Hiraga K; Minami H; Hashimoto H; Yamamoto M; Wakabayashi K; Shimizu T; Sato M
[Ad] Endereço:From the Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
[Ti] Título:Structural basis of autoinhibition and activation of the DNA-targeting ADP-ribosyltransferase pierisin-1.
[So] Source:J Biol Chem;292(37):15445-15455, 2017 Sep 15.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ADP-ribosyltransferases transfer the ADP-ribose moiety of ßNAD to an acceptor molecule, usually a protein that modulates the function of the acceptor. Pierisin-1 is an ADP-ribosyltransferase from the cabbage butterfly and is composed of N-terminal catalytic and C-terminal ricin B-like domains. Curiously, it ADP-ribosylates the DNA duplex, resulting in apoptosis of various cancer cells, which has raised interest in pierisin-1 as an anti-cancer agent. However, both the structure and the mechanism of DNA ADP-ribosylation are unclear. Here, we report the crystal structures of the N-terminal catalytic domain of pierisin-1, its complex with ßNAD , and the catalytic domain with the linker connecting it to the ricin B-like domains. We found that the catalytic domain possesses a defined, positively charged region on the molecular surface but that its overall structure is otherwise similar to those of protein-targeting ADP-ribosyltransferases. Electrophoretic mobility shift assays and site-directed mutagenesis indicated that pierisin-1 binds double-stranded but not single-stranded DNA and that Lys , Lys , and Lys , which are found in a loop, and Arg and Arg , located in a basic cleft near the loop, are required for DNA binding. Furthermore, the structure of the catalytic domain with the linker revealed an autoinhibitory mechanism in which the linker occupies and blocks both the ßNAD - and DNA-binding sites, suggesting that proteolytic cleavage to remove the linker is necessary for enzyme catalysis. Our study provides a structural basis for the DNA-acceptor specificity of pierisin-1 and reveals that a self-regulatory mechanism is required for its activity.
[Mh] Termos MeSH primário: ADP Ribose Transferases/metabolismo
Borboletas/enzimologia
DNA/metabolismo
Precursores Enzimáticos/metabolismo
Proteínas de Insetos/metabolismo
Modelos Moleculares
NAD/metabolismo
Processamento de Proteína Pós-Traducional
[Mh] Termos MeSH secundário: ADP Ribose Transferases/química
ADP Ribose Transferases/genética
Substituição de Aminoácidos
Animais
Sítios de Ligação
Biocatálise
Domínio Catalítico
Cristalografia por Raios X
DNA/química
Ensaio de Desvio de Mobilidade Eletroforética
Ativação Enzimática
Precursores Enzimáticos/química
Precursores Enzimáticos/genética
Proteínas de Insetos/química
Proteínas de Insetos/genética
Mutagênese Sítio-Dirigida
Mutação
NAD/química
Fragmentos de Peptídeos/química
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/metabolismo
Conformação Proteica
Domínios e Motivos de Interação entre Proteínas
Proteólise
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/metabolismo
Homologia Estrutural de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Precursors); 0 (Insect Proteins); 0 (Peptide Fragments); 0 (Recombinant Fusion Proteins); 0 (pierisin protein, insect); 0U46U6E8UK (NAD); 9007-49-2 (DNA); EC 2.4.2.- (ADP Ribose Transferases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.776641



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