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[PMID]:28459996
[Au] Autor:Qiu L; Zhang B; Liu L; Wang X; Lei C; Lin Y; Zhao J; Ma W
[Ad] Endereço:National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Wuhan, China.
[Ti] Título:The Role of p38 MAPK, JNK, and ERK in Antibacterial Responses of Chilo suppressalis (Lepidoptera: Crambidae).
[So] Source:J Econ Entomol;110(4):1460-1464, 2017 08 01.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The mitogen-activated protein kinases (MAPKs) are conserved signal transduction pathways and broadly responsible for bacterial infection from yeast to mammals, and virus, fungi, and bacteria, specifically Bacillus thuringiensis, to insects. But little is known about the MAPK pathways in antibacterial responses in Chilo suppressalis (Walker), an important lepidopteran pest of rice. In this study, we used the bacteria of Bacillus thuringiensis, Escherichia coli, and Staphyloccocus aureus to infect C. suppressalis larvae, and the responses of MAPK pathways were analyzed. The results showed that E. coli infection induced the up-regulated expression of Csp38 and CsERK1 at 24 h postinfection (pi). Meanwhile, injection of B. thuringiensis and S. aureus resulted in strong activation of CsJNK phosphorylation at 3 h pi. These results suggest that MAPK signaling pathways play important functional roles in antibacterial responses in C. suppressalis larvae.
[Mh] Termos MeSH primário: Bacillus thuringiensis/fisiologia
Escherichia coli/fisiologia
Imunidade Inata
Proteínas de Insetos/genética
Proteínas Quinases Ativadas por Mitógeno/genética
Mariposas/imunologia
Staphylococcus aureus/fisiologia
[Mh] Termos MeSH secundário: Animais
MAP Quinases Reguladas por Sinal Extracelular/genética
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Proteínas de Insetos/metabolismo
Proteínas Quinases JNK Ativadas por Mitógeno/genética
Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo
Larva/genética
Larva/crescimento & desenvolvimento
Larva/imunologia
Larva/microbiologia
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Mariposas/genética
Mariposas/crescimento & desenvolvimento
Mariposas/microbiologia
Transdução de Sinais
Proteínas Quinases p38 Ativadas por Mitógeno/genética
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insect Proteins); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox126


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[PMID]:29360856
[Au] Autor:Yang Y; Xu H; Lu Y; Wang C; Lu Z
[Ad] Endereço:State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
[Ti] Título:Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin.
[So] Source:PLoS One;13(1):e0191686, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cnaphalocrocis medinalis (Guenée) is one of the important insect pests in rice field. Bt agents were recommended in the C. medinalis control and Bt rice is bred as a tactic to control this insect. However, the tolerance or resistance of insect to Bt protein is a main threat to the application of Bt protein. In order to investigate the response of C. medinalis transcriptome in defending a Cry1C toxin, high-through RNA-sequencing was carried in the C. medinalis larvae treated with and without Cry1C toxin. A total of 35,586 high-quality unigenes was annotated in the transcriptome of C. medinalis midgut. The comparative analysis identified 6,966 differently expressed unigenes (DEGs) between the two treatments. GO analysis showed that these genes involved in proteolysis and extracellular region. Among these DEGs, carboxylesterase, glutathione S-transferase and P450 were differently expressed in the treated C. medinalis midgut. Furthermore, trypsin, chymotrypsin, and carboxypeptidase were identified in DEGs, and most of them up-regulated. In addition, thirteen ABC transporters were downregulated and three upregulated in Cry1C-treated C. medinalis midgut. Based on the pathway analysis, antigen processing and presentation pathway, and chronic myeloid leukemia pathway were significant in C. medinalis treated with Cry1C toxin. These results indicated that serine protease, detoxification enzymes and ABC transporter, antigen processing and presentation pathway, and chronic myeloid leukemia pathway may involved in the response of C. medinalis to Cry1C toxin. This study provides a transcriptomal foundation for the identification and functional characterization of genes involved in the toxicity of Bt Cry protein against C. medinalis, and provides potential clues to the studies on the tolerance or resistance of an agriculturally important insect pest C. medinalis to Cry1C toxin.
[Mh] Termos MeSH primário: Proteínas de Bactérias/toxicidade
Endotoxinas/toxicidade
Proteínas Hemolisinas/toxicidade
Mariposas/efeitos dos fármacos
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Mariposas/genética
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Endotoxins); 0 (Hemolysin Proteins); 0 (insecticidal crystal protein, Bacillus Thuringiensis)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191686


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[PMID]:29401571
[Au] Autor:Kryukov VY; Rotskaya UN; Yaroslavtseva ON; Elisaphenko EA; Duisembekov BA; Glupov VV
[Ti] Título:[Phenotypic and genetic changes of entomopathogenic ascomycete Beauveria Bassiana under passaging through various hosts].
[So] Source:Parazitologiia;51(1):3-14, 2017 Jan-Feb.
[Is] ISSN:0031-1847
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Phenotypic and genetic estimations of entomopathogenic ascomycete B.bassiana (strain Sar-31) after 6-passaging through four hosts were shown. Increasing of virulence, changes in morpho-cultural characteristics and variations in Inter Simple Sequence Repeats (ISSR) assay between initial and reisolated cultures were registered. Six passages of entomopathogenic ascomycete Beauveria bassiana (strain Sar-31) through four hosts (Galleria mellonella, Tenebrio molitor, Leptinotarsa decemlineata, Locusta migratoria) and following estimation of phenotypic and genetic differences of the initial strain and reisolated cultures were conducted. The passaging of strain through certain host led to increasing of virulence for both this host and other test-insects. Unidirectional changes of morpho-cultural characteristics: colonies pigmentation and relief strengthening, increasing of conidia production and lipolytic activity were registered in all passaged cultures. Genetic analysis with 6 ISSR markers revealed variations between initial and reisolated cultures in 3 markers. Taken together, the results of this study help us understand potential ways of fungi strains changes during epizootic process and possibilities of ISSR assay applying for investigation of pathogen transmission.
[Mh] Termos MeSH primário: Beauveria/genética
Beauveria/patogenicidade
Proteínas Fúngicas/genética
Especificidade de Hospedeiro
Esporos Fúngicos/genética
Esporos Fúngicos/patogenicidade
[Mh] Termos MeSH secundário: Animais
Beauveria/enzimologia
Beauveria/crescimento & desenvolvimento
Coleópteros/microbiologia
Proteínas Fúngicas/metabolismo
Marcadores Genéticos
Genótipo
Lipólise
Locusta migratoria/microbiologia
Repetições de Microssatélites
Mariposas/microbiologia
Fenótipo
Pigmentação/genética
Inoculações Seriadas
Esporos Fúngicos/enzimologia
Esporos Fúngicos/crescimento & desenvolvimento
Tenebrio/microbiologia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Genetic Markers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180206
[St] Status:MEDLINE


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[PMID]:29251469
[Au] Autor:Kaur R; Joshi N; Virk JS; Sharma S
[Ti] Título:Evaluation of Pseudomonas fluorescens for the management of tomato early blight disease and fruit borer.
[So] Source:J Environ Biol;37(5):869-872, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Early blight disease and fruit damage by Helicoverpa armigera are serious problems of tomato causing heavy losses in the yield. An experiment was conducted to evaluate the efficacy of talc based formulation of antagonist Pseudomonas flourescens (Psf) delivered through two different forms of substrate, farmyard manure (FYM) and vermicompost, for the management of tomato early blight disease. Two treatments of Psf, were selected Solarization of nursery soil + seeds and seedlings root dip treatment with Psf formulation + Use of FYM colonized with Psf formulation; Solarization of nursery soil + seed and seedlings root dip treatment with Psf formulation + Use of vermicompost colonized with Psf formulation. These were compared with farmer's practice and untreated control. The impact of all these treatments on tomato fruit borer, H. armigera was also studied. There was not much variation in plant growth parameters (plant height and canopy width) between all the treatments. Both biocontrol treatments (17.69% and 141.3 q acre-1; 15.70% and 139.4 q acre-1, respectively), as well as farmer's practice (10.25% and 208.5 q acre-1) were found to be better than untreated control (29.21% and 94.6 q acre-1) in reducing the incidence of early blight disease in tomato and increasing the marketable yield of tomato fruits. However, the farmer's practice was found to be significantly better than both biocontrol treatments of Psf in controlling fruit damage due to disease, as well as fruit borer. The effect of both bioagents enriched formulations i.e. FYM+Psf and vermicompost+Psf with respect to disease was significantly at par with each other, whereas they had no impact on the fruit borer incidence. The results indicated that either of biocontrol treatments of P. flourescens using farmyard manure and vermicompost, as delivery substrates, can be considered as one of the component along with chemical control in developing IPM programme for the management of early blight disease in tomato.
[Mh] Termos MeSH primário: Antibiose
Lycopersicon esculentum/microbiologia
Mariposas/microbiologia
Controle Biológico de Vetores
Doenças das Plantas/microbiologia
Pseudomonas fluorescens/fisiologia
[Mh] Termos MeSH secundário: Animais
Compostagem
Esterco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Manure)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


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[PMID]:29177897
[Au] Autor:Silva DB; Bueno VHP; Van Loon JJA; Peñaflor MFGV; Bento JMS; Van Lenteren JC
[Ad] Endereço:Department of Entomology, Federal University of Lavras (UFLA), P.O.Box 3037, Lavras, MG, 37200-000, Brazil.
[Ti] Título:Attraction of Three Mirid Predators to Tomato Infested by Both the Tomato Leaf Mining Moth Tuta absoluta and the Whitefly Bemisia tabaci.
[So] Source:J Chem Ecol;44(1):29-39, 2018 Jan.
[Is] ISSN:1573-1561
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plants emit volatile compounds in response to insect herbivory, which may play multiple roles as defensive compounds and mediators of interactions with other plants, microorganisms and animals. Herbivore-induced plant volatiles (HIPVs) may act as indirect plant defenses by attracting natural enemies of the attacking herbivore. We report here the first evidence of the attraction of three Neotropical mirid predators (Macrolophus basicornis, Engytatus varians and Campyloneuropsis infumatus) toward plants emitting volatiles induced upon feeding by two tomato pests, the leaf miner Tuta absoluta and the phloem feeder Bemisia tabaci, in olfactometer bioassays. Subsequently, we compared the composition of volatile blends emitted by insect-infested tomato plants by collecting headspace samples and analyzing them with GC-FID and GC-MS. Egg deposition by T. absoluta did not make tomato plants more attractive to the mirid predators than uninfested tomato plants. Macrolophus basicornis is attracted to tomato plants infested with either T. absoluta larvae or by a mixture of B. tabaci eggs, nymphs and adults. Engytatus varians and C. infumatus responded to volatile blends released by tomato plants infested with T. absoluta larvae over uninfested plants. Also, multiple herbivory by T. absoluta and B. tabaci did not increase the attraction of the mirids compared to infestation with T. absoluta alone. Terpenoids represented the most important class of compounds in the volatile blends and there were significant differences between the volatile blends emitted by tomato plants in response to attack by T. absoluta, B. tabaci, or by both insects. We, therefore, conclude that all three mirids use tomato plant volatiles to find T. absoluta larvae. Multiple herbivory did neither increase, nor decrease attraction of C. infumatus, E. varians and M. basicornis. By breeding for higher rates of emission of selected terpenes, increased attractiveness of tomato plants to natural enemies may improve the effectiveness of biological control.
[Mh] Termos MeSH primário: Heterópteros/fisiologia
Lycopersicon esculentum/química
Mariposas/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Cromatografia Gasosa-Espectrometria de Massas
Heterópteros/crescimento & desenvolvimento
Larva/efeitos dos fármacos
Larva/fisiologia
Lycopersicon esculentum/metabolismo
Mariposas/crescimento & desenvolvimento
Ninfa/efeitos dos fármacos
Ninfa/fisiologia
Folhas de Planta/química
Folhas de Planta/metabolismo
Comportamento Predatório/efeitos dos fármacos
Análise de Componente Principal
Compostos Orgânicos Voláteis/análise
Compostos Orgânicos Voláteis/química
Compostos Orgânicos Voláteis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Volatile Organic Compounds)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1007/s10886-017-0909-x


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[PMID]:29181624
[Au] Autor:Kitchin D; Bouwer G
[Ad] Endereço:School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, Wits, Johannesburg, 2050, South Africa.
[Ti] Título:Significant differences in the intra-host genetic diversity of Helicoverpa armigera nucleopolyhedrovirus dnapol after serial in vivo passages in the same insect population.
[So] Source:Arch Virol;163(3):713-718, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A denaturing gradient gel electrophoresis assay was used to assess the genetic diversity within a region of the DNA polymerase gene (dnapol) in Helicoverpa armigera nucleopolyhedrovirus (HearNPV) populations over serial in vivo passages. There was no evidence of movement towards a consensus dnapol variant composition in the different host larvae after multiple per os passages. The study showed that the HearNPV variant structure after in vivo passages in the same host population is not necessarily convergent, and that it may be reasonable to expect significant differences in intra-host HearNPV genetic diversity after inoculation of larvae with a genotypically-diverse HearNPV inoculum.
[Mh] Termos MeSH primário: DNA Viral/genética
DNA Polimerase Dirigida por DNA/genética
Mariposas/virologia
Nucleopolyhedrovirus/genética
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Animais
DNA Viral/metabolismo
DNA Polimerase Dirigida por DNA/metabolismo
Eletroforese em Gel de Gradiente Desnaturante
Variação Genética
Larva/virologia
Nucleopolyhedrovirus/classificação
Nucleopolyhedrovirus/enzimologia
Nucleopolyhedrovirus/isolamento & purificação
Filogenia
Inoculações Seriadas
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Proteins); EC 2.7.7.7 (DNA-Directed DNA Polymerase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3621-9


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[PMID]:29193264
[Au] Autor:Kausar S; Abbas MN; Qian C; Zhu B; Gao J; Sun Y; Wang L; Wei G; Liu C
[Ad] Endereço:College of Life Sciences, Anhui Agricultural University, Hefei, China.
[Ti] Título:Role of Antheraea pernyi serpin 12 in prophenoloxidase activation and immune responses.
[So] Source:Arch Insect Biochem Physiol;97(2), 2018 Feb.
[Is] ISSN:1520-6327
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Serine protease inhibitors play a key role in the immune system of invertebrates by controlling proteolytic cascades. Besides its importance, the knowledge on immune functions of serpins in most of insects is fragmentary. In the present study, we identified serpin-12 from Antheraea pernyi encoding a predicted 402 amino acid residue protein (Apserpin-12). We expressed the recombinant protein in Escherichia coli and the purified protein was used for the synthesis of rabbit anti-Apserpin-12 polyclonal antibodies and functional studies. Quantitative real-time ploymerase chain reaction (qRT-PCR) analysis revealed that the knock-down of Apserpin-12 enhanced the prophenoloxidase (PPO) cascade stimulated by Micrococcus luteus in hemolymph, whereas addition of recombinant Apserpin-12 protein along with same elicitor led to down-regulate PPO activation. Following different microbial challenge (E. coli, Beauveria bassiana, M. Luteus, and nuclear polyhedrosis virus), the expression of Apserpin-12 mRNA was induced significantly. Furthermore, the Apserpin-12 double-stranded RNA administration elicited the expression of antimicrobial peptides, while the treatment with recombinant protein suppressed their expression. Tissue profile of Apserpin-12 indicated that it is expressed in all examined tissues, that is, hemolymph, malpighian tubules, midgut, silk gland, integument, and fat body with variation in their transcript levels. We concluded that Apserpin-12 may regulate PPO activation and inhibit the production of antimicrobial peptides in A. pernyi, suggesting important role in its immune system.
[Mh] Termos MeSH primário: Catecol Oxidase/metabolismo
Precursores Enzimáticos/metabolismo
Mariposas/química
Serpinas/isolamento & purificação
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Peptídeos Catiônicos Antimicrobianos/metabolismo
Ativação Enzimática
Escherichia coli
Mariposas/fisiologia
Filogenia
Serpinas/química
Serpinas/genética
Serpinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (Enzyme Precursors); 0 (Serpins); EC 1.10.3.- (pro-phenoloxidase); EC 1.10.3.1 (Catechol Oxidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1002/arch.21435


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[PMID]:29193237
[Au] Autor:Zhan MY; Yang PJ; Rao XJ
[Ad] Endereço:School of Plant Protection, Anhui Agricultural University, Hefei, China.
[Ti] Título:Cloning and analysis of peptidoglycan recognition protein-LC and immune deficiency from the diamondback moth, Plutella xylostella.
[So] Source:Arch Insect Biochem Physiol;97(2), 2018 Feb.
[Is] ISSN:1520-6327
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Peptidoglycan (PGN) exists in both Gram-negative and Gram-positive bacteria as a component of the cell wall. PGN is an important target to be recognized by the innate immune system of animals. PGN recognition proteins (PGRP) are responsible for recognizing PGNs. In Drosophila melanogaster, PGRP-LC and IMD (immune deficiency) are critical for activating the Imd pathway. Here, we report the cloning and analysis of PGRP-LC and IMD (PxPGRP-LC and PxIMD) from diamondback moth, Plutella xylostella (L.), the insect pest of cruciferous vegetables. PxPGRP-LC gene consists of six exons encoding a polypeptide of 308 amino acid residues with a transmembrane region and a PGRP domain. PxIMD cDNA encodes a polypeptide of 251 amino acid residues with a death domain. Sequence comparisons indicate that they are characteristic of Drosophila PGRP-LC and IMD homologs. PxPGRP-LC and PxIMD were expressed in various tissues and developmental stages. Their mRNA levels were affected by bacterial challenges. The PGRP domain of PxPGRP-LC lacks key residues for the amidase activity, but it can recognize two types of PGNs. Overexpression of full-length and deletion mutants in Drosophila S2 cells induced expression of some antimicrobial peptide genes. These results indicate that PxPGRP-LC and PxIMD may be involved in the immune signaling of P. xylostella. This study provides a foundation for further studies of the immune system of P. xylostella.
[Mh] Termos MeSH primário: Proteínas de Transporte/isolamento & purificação
Proteínas de Insetos/isolamento & purificação
Mariposas/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Bactérias
Sequência de Bases
Proteínas de Transporte/química
Proteínas de Transporte/genética
Proteínas de Transporte/metabolismo
Linhagem Celular
Proteínas de Insetos/química
Proteínas de Insetos/genética
Proteínas de Insetos/metabolismo
Mariposas/genética
Mariposas/metabolismo
Peptidoglicano/metabolismo
Filogenia
Conformação Proteica
Alinhamento de Sequência
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Insect Proteins); 0 (Peptidoglycan); 0 (peptidoglycan recognition protein)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1002/arch.21436


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[PMID]:28455184
[Au] Autor:Schweizer F; Heidel-Fischer H; Vogel H; Reymond P
[Ad] Endereço:Department of Plant Molecular Biology, University of Lausanne, 1015 Lausanne, Switzerland.
[Ti] Título:Arabidopsis glucosinolates trigger a contrasting transcriptomic response in a generalist and a specialist herbivore.
[So] Source:Insect Biochem Mol Biol;85:21-31, 2017 06.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytophagous insects have to deal with toxic defense compounds from their host plants. Although it is known that insects have evolved genes and mechanisms to detoxify plant allochemicals, how specialist and generalist precisely respond to specific secondary metabolites at the molecular level is less understood. Here we studied the larval performance and transcriptome of the generalist moth Heliothis virescens and the specialist butterfly Pieris brassicae feeding on Arabidopsis thaliana genotypes with different glucosinolate (GS) levels. H. virescens larvae gained significantly more weight on the GS-deficient mutant quadGS compared to wild-type (Col-0) plants. On the contrary, P. brassicae was unaffected by the presence of GS and performed equally well on both genotypes. Strikingly, there was a considerable differential gene expression in H. virescens larvae feeding on Col-0 compared to quadGS. In contrast, compared to H. virescens, P. brassicae displayed a much-reduced transcriptional activation when fed on both plant genotypes. Transcripts coding for putative detoxification enzymes were significantly upregulated in H. virescens, along with digestive enzymes and transposable elements. These data provide an unprecedented view on transcriptional changes that are specifically activated by GS and illustrate differential molecular responses that are linked to adaptation to diet in lepidopteran herbivores.
[Mh] Termos MeSH primário: Borboletas/efeitos dos fármacos
Glucosinolatos/farmacologia
Herbivoria
Mariposas/efeitos dos fármacos
Transcriptoma/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Arabidopsis
Borboletas/genética
Borboletas/metabolismo
Regulação para Baixo
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Inativação Metabólica/genética
Proteínas de Insetos/metabolismo
Larva/efeitos dos fármacos
Mariposas/genética
Mariposas/metabolismo
Análise de Sequência de RNA
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glucosinolates); 0 (Insect Proteins); 0 (cuticle proteins, insects); 0 (storage proteins, Insecta)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:29305978
[Au] Autor:Lin J; Xia X; Yu XQ; Shen J; Li Y; Lin H; Tang S; Vasseur L; You M
[Ad] Endereço:State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Vocational College of Bioengin
[Ti] Título:Gene expression profiling provides insights into the immune mechanism of Plutella xylostella midgut to microbial infection.
[So] Source:Gene;647:21-30, 2018 Mar 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Insect gut immunity plays a key role in defense against microorganism infection. The knowledge of insect gut immunity has been obtained mostly from Drosophila melanogaster. Little is known about gut immunity in the diamondback moth, Plutella xylostella (L.), a pest destroying cruciferous crops worldwide. In this study, expressions of the immune-related genes in the midgut of P. xylostella orally infected with Staphylococcus aureus, Escherichia coli and Pichia pastoris were profiled by RNA-seq and qRT-PCR approaches. The results revealed that the Toll, IMD, JNK and JAK-STAT pathways and possibly the prophenoloxidase activation system in P. xylostella could be activated by oral infections, and moricins, gloverins and lysozyme2 might act as important effectors against microorganisms. Subsequent knock-down of IMD showed that this gene was involved in regulating the expression of down-stream genes in the IMD pathway. Our work indicates that the Toll, IMD, JNK and JAK-STAT pathways may synergistically modulate immune responses in the P. xylostella midgut, implying a complex and diverse immune system in the midgut of insects.
[Mh] Termos MeSH primário: Sistema Digestório/microbiologia
Infecções por Escherichia coli/genética
Lepidópteros/genética
Lepidópteros/imunologia
Micoses/genética
Infecções Estafilocócicas/genética
Transcriptoma/genética
[Mh] Termos MeSH secundário: Animais
Escherichia coli/imunologia
Infecções por Escherichia coli/imunologia
Perfilação da Expressão Gênica/métodos
Proteínas de Insetos/genética
Lepidópteros/microbiologia
Mariposas/genética
Mariposas/imunologia
Mariposas/microbiologia
Micoses/imunologia
Pichia/imunologia
Transdução de Sinais/genética
Transdução de Sinais/imunologia
Infecções Estafilocócicas/imunologia
Staphylococcus aureus/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE



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