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Pesquisa : B01.050.500.308.237.700 [Categoria DeCS]
Referências encontradas : 102 [refinar]
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[PMID]:29177293
[Au] Autor:Yuan M; Ma X; Jiang T; Gao Y; Cui Y; Zhang C; Yang X; Huang Y; Du L; Yampolsky I; Li M
[Ad] Endereço:Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, Shandong 250012, China. mli@sdu.edu.cn.
[Ti] Título:Prolonged bioluminescence imaging in living cells and mice using novel pro-substrates for Renilla luciferase.
[So] Source:Org Biomol Chem;15(48):10238-10244, 2017 Dec 13.
[Is] ISSN:1477-0539
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The prodrug or caged-luciferin strategy affords an excellent platform for persistent bioluminescence imaging. In the current work, we designed and synthesized ten novel pro-substrates for Renilla luciferase by introducing ester protecting groups of different sizes into the carbonyl group of the free luciferin 1. Taking advantage of intracellular esterases, lipases, and nucleophilic substances, the ester protecting groups were hydrolyzed, resulting in the release of a free luciferin and a bioluminescence signal turn-on. Among the tested pro-substrates, the butyryloxymethyl luciferin 7 exhibited low cytotoxicity and a prolonged luminescence signal both in cellulo and in vivo. Therefore, the butyryloxymethyl luciferin 7 can act as a promising substrate for noninvasive extended imaging in diagnostic and therapeutic fields.
[Mh] Termos MeSH primário: Luciferina de Vaga-Lumes/química
Luciferases/análise
Medições Luminescentes
Renilla/enzimologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Feminino
Luciferina de Vaga-Lumes/síntese química
Luciferina de Vaga-Lumes/farmacologia
Seres Humanos
Luciferases/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Nus
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
5TBB02N29K (Firefly Luciferin); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1039/c7ob01656e


  2 / 102 MEDLINE  
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[PMID]:27956170
[Au] Autor:Smirnov NA; Akopov SB; Didych DA; Nikolaev LG
[Ad] Endereço:Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
[Ti] Título:In trans promoter activation by enhancers in transient transfection.
[So] Source:Gene;603:15-20, 2017 Mar 01.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Earlier, it was reported that the strong cytomegalovirus enhancer can activate the cytomegalovirus promoter in trans, i.e. as a separate plasmid co-transfected with a promoter-reporter gene construct. Here we demonstrate that the ability of enhancers to activate promoters in trans in transient transfection experiments is a property of not only viral regulatory elements but also of various genomic enhancers and promoters. Enhancer-promoter activation in trans is promoter- and cell type-specific, and accompanied by physical interaction between promoter and enhancer as revealed by chromosome conformation capture assays. Thus, promoter activation in transient co-transfection of promoters and enhancers shares a number of important traits with long-distance promoter activation by enhancers in living cells and may therefore serve as a model of this fundamental cellular process.
[Mh] Termos MeSH primário: Elementos Facilitadores Genéticos
Plasmídeos/metabolismo
Regiões Promotoras Genéticas
Transativadores/genética
Ativação Transcricional
Transfecção/métodos
[Mh] Termos MeSH secundário: Animais
Citomegalovirus/genética
Citomegalovirus/metabolismo
Vaga-Lumes/enzimologia
Vaga-Lumes/genética
Genes Reporter
Células HeLa
Células Hep G2
Seres Humanos
Luciferases/genética
Luciferases/metabolismo
Especificidade de Órgãos
Plasmídeos/química
Renilla/enzimologia
Renilla/genética
Transativadores/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Trans-Activators); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170202
[Lr] Data última revisão:
170202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE


  3 / 102 MEDLINE  
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[PMID]:27871386
[Au] Autor:Rahnama S; Saffar B; Kahrani ZF; Nazari M; Emamzadeh R
[Ad] Endereço:Department of Biology, Nourdanesh Institute of Higher Education, Meymeh, Iran.
[Ti] Título:Super RLuc8: A novel engineered Renilla luciferase with a red-shifted spectrum and stable light emission.
[So] Source:Enzyme Microb Technol;96:60-66, 2017 Jan.
[Is] ISSN:1879-0909
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Renilla luciferase is a bioluminescent enzyme which is broadly used as a reporter protein in molecular biosensors. In this study, a novel luciferase with desired light emission wavelength and thermostability is reported. The results indicated that the new luciferase, namely super RLuc8, had a red-shifted spectrum and showed stable light emission. Super RLuc8 showed a 10-fold (p-value=0.0084) increase in the thermostability at 37°C after 20min incubation, in comparison to the native enzyme. The optimum temperature of the mutant increased from 30 to 37°C. Molecular dynamics simulation analysis indicated that the increased thermostability was most probably caused by a better structural compactness and more local rigidity in the regions out of the emitter site.
[Mh] Termos MeSH primário: Luciferases de Renilla/química
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Animais
Biotecnologia
Estabilidade Enzimática/genética
Cinética
Luciferases de Renilla/genética
Luciferases de Renilla/metabolismo
Medições Luminescentes
Simulação de Dinâmica Molecular
Mutagênese Sítio-Dirigida
Engenharia de Proteínas
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Renilla/enzimologia
Renilla/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins); EC 1.13.12.5 (Luciferases, Renilla)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170530
[Lr] Data última revisão:
170530
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE


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[PMID]:26789132
[Au] Autor:Zhang H; Bai H; Jiang T; Ma Z; Cheng Y; Zhou Y; Du L; Li M
[Ad] Endereço:Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, Shandong 250012, China. mli@sdu.edu.cn.
[Ti] Título:Quenching the firefly bioluminescence by various ions.
[So] Source:Photochem Photobiol Sci;15(2):244-9, 2016 Feb.
[Is] ISSN:1474-9092
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The luciferase reporter gene assay system is broadly applied in various biomedical aspects, including signaling pathway dissection, transcriptional activity analysis, and genetic toxicity testing. It significantly improves the experimental accuracy and reduces the experimental error by the addition of an internal control. In the current research, we discovered some specific ions that could selectively inhibit firefly luciferase while having a negligible effect on renilla luciferase in vitro in the dual-reporter gene assay. We showed that these ionic compounds had a high potential of being utilized as quench-and-activate reagents in the dual-reporter assay. Furthermore, results from kinetic studies on ion-mediated quenching effects indicated that different ions have distinct inhibition modes. Our study is anticipated to guide a more affordable design of quench-and-activate reagents in biomedicine and pharmaceutical analysis.
[Mh] Termos MeSH primário: Vaga-Lumes/enzimologia
Íons/metabolismo
Luciferases de Vaga-Lume/metabolismo
Luciferases de Renilla/metabolismo
Substâncias Luminescentes/metabolismo
Renilla/enzimologia
[Mh] Termos MeSH secundário: Animais
Ensaios Enzimáticos
Vaga-Lumes/genética
Genes Reporter
Luciferases de Vaga-Lume/antagonistas & inibidores
Luciferases de Vaga-Lume/genética
Luciferases de Renilla/antagonistas & inibidores
Luciferases de Renilla/genética
Luminescência
Renilla/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ions); 0 (Luminescent Agents); EC 1.13.12.5 (Luciferases, Renilla); EC 1.13.12.7 (Luciferases, Firefly)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160121
[St] Status:MEDLINE
[do] DOI:10.1039/c5pp00432b


  5 / 102 MEDLINE  
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[PMID]:26322739
[Au] Autor:Kim SB; Nishihara R; Citterio D; Suzuki K
[Ad] Endereço:Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST) , 16-1 Onogawa, Tsukuba 305-8569, Japan.
[Ti] Título:Genetically Encoded Molecular Tension Probe for Tracing Protein-Protein Interactions in Mammalian Cells.
[So] Source:Bioconjug Chem;27(2):354-62, 2016 Feb 17.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Optical imaging of protein-protein interactions (PPIs) facilitates comprehensive elucidation of intracellular molecular events. We demonstrate an optical measure for visualizing molecular tension triggered by any PPI in mammalian cells. Twenty-three kinds of candidate designs were fabricated, in which a full-length artificial luciferase (ALuc) was sandwiched between two model proteins of interest, e.g., FKBP and FRB. One of the designs greatly enhanced the bioluminescence in response to varying concentrations of rapamycin. It is confirmed with negative controls that the elevated bioluminescence is solely motivated from the molecular tension. The probe design was further modified toward eliminating the C-terminal end of ALuc and was found to improve signal-to-background ratios, named "a combinational probe". The utilities were elucidated with detailed substrate selectivity, bioluminescence imaging of live cells, and different PPI models. This study expands capabilities of luciferases as a tool for analyses of molecular dynamics and cell signaling in living subjects.
[Mh] Termos MeSH primário: Luciferases de Renilla/metabolismo
Sondas Moleculares/metabolismo
Mapeamento de Interação de Proteínas/métodos
Serina-Treonina Quinases TOR/metabolismo
Proteínas de Ligação a Tacrolimo/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Fenômenos Biomecânicos
Células COS
Cercopithecus aethiops
Seres Humanos
Luciferases de Renilla/química
Medições Luminescentes/métodos
Sondas Moleculares/química
Dados de Sequência Molecular
Ligação Proteica
Estrutura Terciária de Proteína
Renilla/química
Renilla/enzimologia
Serina-Treonina Quinases TOR/química
Proteínas de Ligação a Tacrolimo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Molecular Probes); EC 1.13.12.5 (Luciferases, Renilla); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 5.2.1.- (Tacrolimus Binding Proteins)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150901
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.5b00421


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[PMID]:26212314
[Au] Autor:Luckert C; Hessel S; Lampen A; Braeuning A
[Ad] Endereço:Federal Institute for Risk Assessment, 10589 Berlin, Germany; Department of Nutritional Toxicology, Institute of Nutritional Science, University of Potsdam, 14558 Nuthetal, Germany.
[Ti] Título:Utility of an appropriate reporter assay: Heliotrine interferes with GAL4/upstream activation sequence-driven reporter gene systems.
[So] Source:Anal Biochem;487:45-8, 2015 Oct 15.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reporter gene assays are widely used for the assessment of transcription factor activation following xenobiotic exposure of cells. A critical issue with such assays is the possibility of interference of test compounds with the test system, for example, by direct inhibition of the reporter enzyme. Here we show that the pyrrolizidine alkaloid heliotrine interferes with reporter signals derived from GAL4-based nuclear receptor transactivation assays by a mechanism independent of luciferase enzyme inhibition. These data highlight the necessity to conduct proper control experiments in order to avoid perturbation of reporter assays by test chemicals.
[Mh] Termos MeSH primário: Genes Reporter/efeitos dos fármacos
Luciferases de Vaga-Lume/antagonistas & inibidores
Luciferases de Renilla/antagonistas & inibidores
Alcaloides de Pirrolizidina/farmacologia
[Mh] Termos MeSH secundário: Animais
Vaga-Lumes
Genes Reporter/genética
Luciferases de Vaga-Lume/genética
Luciferases de Vaga-Lume/metabolismo
Luciferases de Renilla/genética
Luciferases de Renilla/metabolismo
Alcaloides de Pirrolizidina/química
RNA Mensageiro/antagonistas & inibidores
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Renilla
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Pyrrolizidine Alkaloids); 0 (RNA, Messenger); EC 1.13.12.5 (Luciferases, Renilla); EC 1.13.12.7 (Luciferases, Firefly); ZYB88Y4FUZ (heliotrine)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150728
[St] Status:MEDLINE


  7 / 102 MEDLINE  
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[PMID]:25831507
[Au] Autor:Takai A; Nakano M; Saito K; Haruno R; Watanabe TM; Ohyanagi T; Jin T; Okada Y; Nagai T
[Ad] Endereço:Laboratories for Cell Polarity Regulation.
[Ti] Título:Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging.
[So] Source:Proc Natl Acad Sci U S A;112(14):4352-6, 2015 Apr 07.
[Is] ISSN:1091-6490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fluorescence live imaging has become an essential methodology in modern cell biology. However, fluorescence requires excitation light, which can sometimes cause potential problems, such as autofluorescence, phototoxicity, and photobleaching. Furthermore, combined with recent optogenetic tools, the light illumination can trigger their unintended activation. Because luminescence imaging does not require excitation light, it is a good candidate as an alternative imaging modality to circumvent these problems. The application of luminescence imaging, however, has been limited by the two drawbacks of existing luminescent protein probes, such as luciferases: namely, low brightness and poor color variants. Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern. The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein. The brightness of these cyan and orange Nano-lanterns was ∼20 times brighter than wild-type Renilla luciferase, which allowed us to perform multicolor live imaging of intracellular submicron structures. The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution, and the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage. In addition, we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
[Mh] Termos MeSH primário: Luciferases/química
Luminescência
Proteínas Luminescentes/química
Proteínas Recombinantes de Fusão/química
[Mh] Termos MeSH secundário: Animais
Cálcio/metabolismo
Linhagem Celular
DNA/química
Cães
Células-Tronco Embrionárias/citologia
Endossomos/metabolismo
Transferência Ressonante de Energia de Fluorescência
Adesões Focais
Regulação da Expressão Gênica
Luciferases de Renilla/metabolismo
Camundongos
Dados de Sequência Molecular
Oligonucleotídeos/química
Peroxissomos/metabolismo
Regiões Promotoras Genéticas
Renilla
Vinculina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Luminescent Proteins); 0 (Oligonucleotides); 0 (Recombinant Fusion Proteins); 125361-02-6 (Vinculin); 9007-49-2 (DNA); EC 1.13.12.- (Luciferases); EC 1.13.12.- (nano-lantern protein); EC 1.13.12.5 (Luciferases, Renilla); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150402
[St] Status:MEDLINE
[do] DOI:10.1073/pnas.1418468112


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[PMID]:25677617
[Au] Autor:Afshari A; Uhde-Stone C; Lu B
[Ad] Endereço:Department of Biological Sciences, California State University, East Bay, 25800 Carlos Bee Blvd, Hayward, CA 94542, USA. Electronic address: Afshari.amirali@gmail.com.
[Ti] Título:A cooled CCD camera-based protocol provides an effective solution for in vitro monitoring of luciferase.
[So] Source:Biochem Biophys Res Commun;458(3):543-8, 2015 Mar 13.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Luciferase assay has become an increasingly important technique to monitor a wide range of biological processes. However, the mainstay protocols require a luminometer to acquire and process the data, therefore limiting its application to specialized research labs. To overcome this limitation, we have developed an alternative protocol that utilizes a commonly available cooled charge-coupled device (CCCD), instead of a luminometer for data acquiring and processing. By measuring activities of different luciferases, we characterized their substrate specificity, assay linearity, signal-to-noise levels, and fold-changes via CCCD. Next, we defined the assay parameters that are critical for appropriate use of CCCD for different luciferases. To demonstrate the usefulness in cultured mammalian cells, we conducted a case study to examine NFκB gene activation in response to inflammatory signals in human embryonic kidney cells (HEK293 cells). We found that data collected by CCCD camera was equivalent to those acquired by luminometer, thus validating the assay protocol. In comparison, The CCCD-based protocol is readily amenable to live-cell and high-throughput applications, offering fast simultaneous data acquisition and visual and quantitative data presentation. In conclusion, the CCCD-based protocol provides a useful alternative for monitoring luciferase reporters. The wide availability of CCCD will enable more researchers to use luciferases to monitor and quantify biological processes.
[Mh] Termos MeSH primário: Luciferases de Vaga-Lume/análise
Luciferases de Renilla/análise
Substâncias Luminescentes/análise
Medições Luminescentes/instrumentação
[Mh] Termos MeSH secundário: Animais
Vaga-Lumes/enzimologia
Genes Reporter
Células HEK293
Ensaios de Triagem em Larga Escala/instrumentação
Seres Humanos
Luciferases de Vaga-Lume/genética
Luciferases de Renilla/genética
Substâncias Luminescentes/metabolismo
Proteínas Recombinantes/análise
Proteínas Recombinantes/genética
Renilla/enzimologia
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Luminescent Agents); 0 (Recombinant Proteins); EC 1.13.12.5 (Luciferases, Renilla); EC 1.13.12.7 (Luciferases, Firefly)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:150309
[Lr] Data última revisão:
150309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150214
[St] Status:MEDLINE


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[PMID]:25595987
[Au] Autor:De Francesco PN; Valdivia S; Cabral A; Reynaldo M; Raingo J; Sakata I; Osborne-Lawrence S; Zigman JM; Perelló M
[Ad] Endereço:Laboratory of Neurophysiology, Multidisciplinary Institute of Cell Biology [Argentine Research Council (CONICET) and Scientific Research Commission, Province of Buenos Aires (CIC-PBA)], La Plata, Buenos Aires, Argentina.
[Ti] Título:Neuroanatomical and functional characterization of CRF neurons of the amygdala using a novel transgenic mouse model.
[So] Source:Neuroscience;289:153-65, 2015 Mar 19.
[Is] ISSN:1873-7544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The corticotropin-releasing factor (CRF)-producing neurons of the amygdala have been implicated in behavioral and physiological responses associated with fear, anxiety, stress, food intake and reward. To overcome the difficulties in identifying CRF neurons within the amygdala, a novel transgenic mouse line, in which the humanized recombinant Renilla reniformis green fluorescent protein (hrGFP) is under the control of the CRF promoter (CRF-hrGFP mice), was developed. First, the CRF-hrGFP mouse model was validated and the localization of CRF neurons within the amygdala was systematically mapped. Amygdalar hrGFP-expressing neurons were located primarily in the interstitial nucleus of the posterior limb of the anterior commissure, but also present in the central amygdala. Secondly, the marker of neuronal activation c-Fos was used to explore the response of amygdalar CRF neurons in CRF-hrGFP mice under different experimental paradigms. C-Fos induction was observed in CRF neurons of CRF-hrGFP mice exposed to an acute social defeat stress event, a fasting/refeeding paradigm or lipopolysaccharide (LPS) administration. In contrast, no c-Fos induction was detected in CRF neurons of CRF-hrGFP mice exposed to restraint stress, forced swimming test, 48-h fasting, acute high-fat diet (HFD) consumption, intermittent HFD consumption, ad libitum HFD consumption, HFD withdrawal, conditioned HFD aversion, ghrelin administration or melanocortin 4 receptor agonist administration. Thus, this study fully characterizes the distribution of amygdala CRF neurons in mice and suggests that they are involved in some, but not all, stress or food intake-related behaviors recruiting the amygdala.
[Mh] Termos MeSH primário: Tonsila do Cerebelo/citologia
Tonsila do Cerebelo/fisiologia
Hormônio Liberador da Corticotropina/metabolismo
Neurônios/citologia
Neurônios/fisiologia
[Mh] Termos MeSH secundário: Proteínas de Anfíbios/genética
Proteínas de Anfíbios/metabolismo
Tonsila do Cerebelo/efeitos dos fármacos
Tonsila do Cerebelo/fisiopatologia
Animais
Dieta Hiperlipídica
Dominação-Subordinação
Ingestão de Alimentos/fisiologia
Jejum/fisiologia
Grelina/administração & dosagem
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Lipopolissacarídeos
Masculino
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Neurônios/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-fos/metabolismo
Receptor Tipo 4 de Melanocortina/antagonistas & inibidores
Receptor Tipo 4 de Melanocortina/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Renilla
Restrição Física
Estresse Psicológico/fisiopatologia
Natação/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amphibian Proteins); 0 (Ghrelin); 0 (Lipopolysaccharides); 0 (MC4R protein, mouse); 0 (Proto-Oncogene Proteins c-fos); 0 (Receptor, Melanocortin, Type 4); 0 (Recombinant Proteins); 147336-22-9 (Green Fluorescent Proteins); 9015-71-8 (Corticotropin-Releasing Hormone)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150118
[St] Status:MEDLINE


  10 / 102 MEDLINE  
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[PMID]:25572456
[Au] Autor:Song H; Oh B; Choi M; Oh J; Lee M
[Ad] Endereço:BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Department of Bioengineering, College of Engineering, Hanyang University , Seoul , Korea.
[Ti] Título:Delivery of anti-microRNA-21 antisense-oligodeoxynucleotide using amphiphilic peptides for glioblastoma gene therapy.
[So] Source:J Drug Target;23(4):360-70, 2015 May.
[Is] ISSN:1029-2330
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Inhibition of microRNA-21 (miR-21) has been shown to promote apoptosis of cancer cells and to reduce tumor size in glioblastoma. However, efficient carriers for antisense-oligodeoxynucleotide (antisense-ODN) against miR-21 have not yet been developed. In this study, the R3V6 peptide (R3V6) was evaluated as a carrier of antisense-ODN. In a gel retardation assay, R3V6 formed a complex with an antisense-ODN. The serum stability assay showed that R3V6 protected it from nucleases more efficiently than polyethylenimine (PEI; 25 kDa, PEI25k). A Renilla luciferase gene with a 3'-untranslated region (3'-UTR) recognizable by miR-21 (psiCHECK2-miR-21-UTR) was constructed for the antisense-ODN assay. psiCHECK2-miR-21-UTR expressed less Renilla luciferase in the cells with a higher level of miR-21 due to the effect of miR-21. In an in vitro transfection assay, the R3V6 peptide delivered anti-miR-21 antisense-ODN into cells more efficiently than PEI (25 kDa, PEI25k) and lipofectamine. As a result, antisense-ODN/R3V6 complex inhibited miR-21 and increased Renilla luciferase expression more efficiently than antisense-ODN/PEI25k or antisense-ODN/Lipofectamine complexes in both C6 and A172 glioblastoma cells. Furthermore, the antisense-ODN/R3V6 complexes reduced the level of miR-21 and induced apoptosis of glioblastoma cells. These results suggest that the R3V6 peptide may be a useful carrier of antisense-ODN for glioblastoma gene therapy.
[Mh] Termos MeSH primário: Terapia Genética/métodos
Glioblastoma/terapia
MicroRNAs/genética
Oligonucleotídeos Antissenso/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Linhagem Celular Tumoral
Glioblastoma/genética
Seres Humanos
Luciferases/genética
Peptídeos/química
Polietilenoimina/química
Ratos
Renilla/genética
Transfecção/métodos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MIRN21 microRNA, human); 0 (MicroRNAs); 0 (Oligonucleotides, Antisense); 0 (Peptides); 0 (mirn21 microRNA, rat); 9002-98-6 (Polyethyleneimine); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150414
[Lr] Data última revisão:
150414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150110
[St] Status:MEDLINE
[do] DOI:10.3109/1061186X.2014.1000336



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