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Pesquisa : B01.050.500.308.237.750 [Categoria DeCS]
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[PMID]:28468269
[Au] Autor:Logashina YA; Solstad RG; Mineev KS; Korolkova YV; Mosharova IV; Dyachenko IA; Palikov VA; Palikova YA; Murashev AN; Arseniev AS; Kozlov SA; Stensvåg K; Haug T; Andreev YA
[Ad] Endereço:Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia. yulia.logashina@gmail.com.
[Ti] Título:New Disulfide-Stabilized Fold Provides Sea Anemone Peptide to Exhibit Both Antimicrobial and TRPA1 Potentiating Properties.
[So] Source:Toxins (Basel);9(5), 2017 Apr 29.
[Is] ISSN:2072-6651
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A novel bioactive peptide named τ-AnmTx Ueq 12-1 (short name Ueq 12-1) was isolated and characterized from the sea anemone Ueq 12-1 is unique among the variety of known sea anemone peptides in terms of its primary and spatial structure. It consists of 45 amino acids including 10 cysteine residues with an unusual distribution and represents a new group of sea anemone peptides. The 3D structure of Ueq 12-1, determined by NMR spectroscopy, represents a new disulfide-stabilized fold partly similar to the defensin-like fold. Ueq 12-1 showed the dual activity of both a moderate antibacterial activity against Gram-positive bacteria and a potentiating activity on the transient receptor potential ankyrin 1 (TRPA1). Ueq 12-1 is a unique peptide potentiator of the TRPA1 receptor that produces analgesic and anti-inflammatory effects . The antinociceptive properties allow us to consider Ueq 12-1 as a potential analgesic drug lead with antibacterial properties.
[Mh] Termos MeSH primário: Analgésicos
Antibacterianos
Anti-Inflamatórios
Peptídeos
Anêmonas-do-Mar
Canal de Cátion TRPA1/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Analgésicos/química
Analgésicos/isolamento & purificação
Analgésicos/farmacologia
Analgésicos/uso terapêutico
Animais
Antibacterianos/química
Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
Antibacterianos/uso terapêutico
Anti-Inflamatórios/química
Anti-Inflamatórios/isolamento & purificação
Anti-Inflamatórios/farmacologia
Anti-Inflamatórios/uso terapêutico
Dissulfetos/química
Edema/tratamento farmacológico
Peptídeos/química
Peptídeos/isolamento & purificação
Peptídeos/farmacologia
Peptídeos/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Anti-Bacterial Agents); 0 (Anti-Inflammatory Agents); 0 (Disulfides); 0 (Peptides); 0 (TRPA1 Cation Channel)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE


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[PMID]:29373572
[Au] Autor:Friedman LE; Gilmore TD; Finnerty JR
[Ad] Endereço:Department of Biology, Boston University, Boston, MA, United States of America.
[Ti] Título:Intraspecific variation in oxidative stress tolerance in a model cnidarian: Differences in peroxide sensitivity between and within populations of Nematostella vectensis.
[So] Source:PLoS One;13(1):e0188265, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nematostella vectensis is a member of the phylum Cnidaria, a lineage that includes anemones, corals, hydras, and jellyfishes. This estuarine anemone is an excellent model system for investigating the evolution of stress tolerance because it is easy to collect in its natural habitat and to culture in the laboratory, and it has a sequenced genome. Additionally, there is evidence of local adaptation to environmental stress in different N. vectensis populations, and abundant protein-coding polymorphisms have been identified, including polymorphisms in proteins that are implicated in stress responses. N. vectensis can tolerate a wide range of environmental parameters, and has recently been shown to have substantial intraspecific variation in temperature preference. We investigated whether different clonal lines of anemones also exhibit differential tolerance to oxidative stress. N. vectensis populations are continually exposed to reactive oxygen species (ROS) generated during cellular metabolism and by other environmental factors. Fifteen clonal lines of N. vectensis collected from four different estuaries were exposed to hydrogen peroxide. Pronounced differences in survival and regeneration were apparent between clonal lines collected from Meadowlands, NJ, Baruch, SC, and Kingsport, NS, as well as among 12 clonal lines collected from a single Cape Cod marsh. To our knowledge, this is the first example of intraspecific variability in oxidative stress resistance in cnidarians or in any marine animal. As oxidative stress often accompanies heat stress in marine organisms, resistance to oxidative stress could strongly influence survival in warming oceans. For example, while elevated temperatures trigger bleaching in corals, oxidative stress is thought to be the proximal trigger of bleaching at the cellular level.
[Mh] Termos MeSH primário: Estresse Oxidativo
Anêmonas-do-Mar/fisiologia
[Mh] Termos MeSH secundário: Animais
Ecossistema
Estuários
Aquecimento Global
Peróxido de Hidrogênio/toxicidade
Modelos Biológicos
Estresse Oxidativo/efeitos dos fármacos
Regeneração/efeitos dos fármacos
Regeneração/fisiologia
Anêmonas-do-Mar/efeitos dos fármacos
Anêmonas-do-Mar/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188265


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[PMID]:29257953
[Au] Autor:Sigg MA; Menchen T; Lee C; Johnson J; Jungnickel MK; Choksi SP; Garcia G; Busengdal H; Dougherty GW; Pennekamp P; Werner C; Rentzsch F; Florman HM; Krogan N; Wallingford JB; Omran H; Reiter JF
[Ad] Endereço:Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, CA 94158, USA.
[Ti] Título:Evolutionary Proteomics Uncovers Ancient Associations of Cilia with Signaling Pathways.
[So] Source:Dev Cell;43(6):744-762.e11, 2017 12 18.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cilia are organelles specialized for movement and signaling. To infer when during evolution signaling pathways became associated with cilia, we characterized the proteomes of cilia from sea urchins, sea anemones, and choanoflagellates. We identified 437 high-confidence ciliary candidate proteins conserved in mammals and discovered that Hedgehog and G-protein-coupled receptor pathways were linked to cilia before the origin of bilateria and transient receptor potential (TRP) channels before the origin of animals. We demonstrated that candidates not previously implicated in ciliary biology localized to cilia and further investigated ENKUR, a TRP channel-interacting protein identified in the cilia of all three organisms. ENKUR localizes to motile cilia and is required for patterning the left-right axis in vertebrates. Moreover, mutation of ENKUR causes situs inversus in humans. Thus, proteomic profiling of cilia from diverse eukaryotes defines a conserved ciliary proteome, reveals ancient connections to signaling, and uncovers a ciliary protein that underlies development and human disease.
[Mh] Termos MeSH primário: Proteínas Adaptadoras de Transdução de Sinal/metabolismo
Proteínas de Ligação a Calmodulina/metabolismo
Cílios/genética
Cílios/metabolismo
[Mh] Termos MeSH secundário: Proteínas Adaptadoras de Transdução de Sinal/genética
Animais
Proteínas de Ligação a Calmodulina/genética
Técnicas de Cultura de Células
Coanoflagelados/metabolismo
Proteínas Hedgehog/metabolismo
Seres Humanos
Camundongos
Mutação
Organelas/metabolismo
Filogenia
Proteômica/métodos
Receptores Acoplados a Proteínas-G/metabolismo
Anêmonas-do-Mar/metabolismo
Ouriços-do-Mar/metabolismo
Transdução de Sinais/genética
Transdução de Sinais/fisiologia
Canais de Receptores Transientes de Potencial/metabolismo
Xenopus laevis/metabolismo
Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Proteins, Signal Transducing); 0 (Calmodulin-Binding Proteins); 0 (ENKUR protein, human); 0 (Hedgehog Proteins); 0 (Receptors, G-Protein-Coupled); 0 (Transient Receptor Potential Channels)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


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[PMID]:28888696
[Au] Autor:Havrilak JA; Faltine-Gonzalez D; Wen Y; Fodera D; Simpson AC; Magie CR; Layden MJ
[Ad] Endereço:Lehigh University, Department of Biological Sciences, United States.
[Ti] Título:Characterization of NvLWamide-like neurons reveals stereotypy in Nematostella nerve net development.
[So] Source:Dev Biol;431(2):336-346, 2017 11 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The organization of cnidarian nerve nets is traditionally described as diffuse with randomly arranged neurites that show minimal reproducibility between animals. However, most observations of nerve nets are conducted using cross-reactive antibodies that broadly label neurons, which potentially masks stereotyped patterns produced by individual neuronal subtypes. Additionally, many cnidarians species have overt structures such as a nerve ring, suggesting higher levels of organization and stereotypy exist, but mechanisms that generated that stereotypy are unknown. We previously demonstrated that NvLWamide-like is expressed in a small subset of the Nematostella nerve net and speculated that observing a few neurons within the developing nerve net would provide a better indication of potential stereotypy. Here we document NvLWamide-like expression more systematically. NvLWamide-like is initially expressed in the typical neurogenic salt and pepper pattern within the ectoderm at the gastrula stage, and expression expands to include endodermal salt and pepper expression at the planula larval stage. Expression persists in both ectoderm and endoderm in adults. We characterized our NvLWamide-like::mCherry transgenic reporter line to visualize neural architecture and found that NvLWamide-like is expressed in six neural subtypes identifiable by neural morphology and location. Upon completing development the numbers of neurons in each neural subtype are minimally variable between animals and the projection patterns of each subtype are consistent. Furthermore, between the juvenile polyp and adult stages the number of neurons for each subtype increases. We conclude that development of the Nematostella nerve net is stereotyped between individuals. Our data also imply that one aspect of generating adult cnidarian nervous systems is to modify the basic structural architecture generated in the juvenile by increasing neural number proportionally with size.
[Mh] Termos MeSH primário: Rede Nervosa/embriologia
Neurônios/metabolismo
Anêmonas-do-Mar/embriologia
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Ectoderma/metabolismo
Endoderma/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Faringe/inervação
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Anêmonas-do-Mar/genética
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170911
[St] Status:MEDLINE


  5 / 1300 MEDLINE  
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[PMID]:28827097
[Au] Autor:Busengdal H; Rentzsch F
[Ad] Endereço:Sars Centre for Marine Molecular Biology, University of Bergen, Thormøhlensgt. 55, 5006 Bergen, Norway.
[Ti] Título:Unipotent progenitors contribute to the generation of sensory cell types in the nervous system of the cnidarian Nematostella vectensis.
[So] Source:Dev Biol;431(1):59-68, 2017 11 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nervous systems often consist of a large number of different types of neurons which are generated from neural stem and progenitor cells by a series of symmetric and asymmetric divisions. The origin and early evolution of these neural progenitor systems is not well understood. Here we use a cnidarian model organism, Nematostella vectensis, to gain insight into the generation of neural cell type diversity in a non-bilaterian animal. We identify NvFoxQ2d as a transcription factor that is expressed in a population of spatially restricted, proliferating ectodermal cells that are derived from NvSoxB(2)-expressing neural progenitor cells. Using a transgenic reporter line we show that the NvFoxQ2d cells undergo a terminal, symmetric division to generate a morphologically homogeneous population of putative sensory cells. The abundance of these cells, but not their proliferation status is affected by treatment with the γ-secretase inhibitor DAPT, suggesting regulation by Notch signalling. Our data suggest that intermediate progenitor cells and symmetric divisions contribute to the formation of the seemingly simple nervous system of a sea anemone.
[Mh] Termos MeSH primário: Células-Tronco Neurais/citologia
Neurogênese
Anêmonas-do-Mar/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Evolução Molecular
Regulação da Expressão Gênica no Desenvolvimento
Células-Tronco Neurais/metabolismo
Neurogênese/genética
Neurogênese/fisiologia
Filogenia
Receptores Notch/genética
Receptores Notch/metabolismo
Anêmonas-do-Mar/citologia
Anêmonas-do-Mar/genética
Células Receptoras Sensoriais/citologia
Células Receptoras Sensoriais/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Receptors, Notch)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE


  6 / 1300 MEDLINE  
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[PMID]:28818668
[Au] Autor:Amiel AR; Johnston H; Chock T; Dahlin P; Iglesias M; Layden M; Röttinger E; Martindale MQ
[Ad] Endereço:Université Côte d'Azur, CNRS, INSERM, Institute for Research on Cancer and Aging, Nice, France.
[Ti] Título:A bipolar role of the transcription factor ERG for cnidarian germ layer formation and apical domain patterning.
[So] Source:Dev Biol;430(2):346-361, 2017 10 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Germ layer formation and axial patterning are biological processes that are tightly linked during embryonic development of most metazoans. In addition to canonical WNT, it has been proposed that ERK-MAPK signaling is involved in specifying oral as well as aboral territories in cnidarians. However, the effector and the molecular mechanism underlying latter phenomenon is unknown. By screening for potential effectors of ERK-MAPK signaling in both domains, we identified a member of the ETS family of transcription factors, Nverg that is bi-polarily expressed prior to gastrulation. We further describe the crucial role of NvERG for gastrulation, endomesoderm as well as apical domain formation. The molecular characterization of the obtained NvERG knock-down phenotype using previously described as well as novel potential downstream targets, provides evidence that a single transcription factor, NvERG, simultaneously controls expression of two different sets of downstream targets, leading to two different embryonic gene regulatory networks (GRNs) in opposite poles of the developing embryo. We also highlight the molecular interaction of cWNT and MEK/ERK/ERG signaling that provides novel insight into the embryonic axial organization of Nematostella, and show a cWNT repressive role of MEK/ERK/ERG signaling in segregating the endomesoderm in two sub-domains, while a common input of both pathways is required for proper apical domain formation. Taking together, we build the first blueprint for a global cnidarian embryonic GRN that is the foundation for additional gene specific studies addressing the evolution of embryonic and larval development.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica no Desenvolvimento
Camadas Germinativas/crescimento & desenvolvimento
Anêmonas-do-Mar/genética
Fatores de Transcrição/fisiologia
[Mh] Termos MeSH secundário: Animais
Padronização Corporal
DNA Complementar/genética
Embrião não Mamífero/metabolismo
Embrião não Mamífero/ultraestrutura
Fatores de Crescimento de Fibroblastos/fisiologia
Gastrulação/genética
Técnicas de Silenciamento de Genes
Redes Reguladoras de Genes
Camadas Germinativas/metabolismo
Sistema de Sinalização das MAP Quinases
Mesoderma/metabolismo
Anêmonas-do-Mar/embriologia
Anêmonas-do-Mar/ultraestrutura
Via de Sinalização Wnt
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Transcription Factors); 62031-54-3 (Fibroblast Growth Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE


  7 / 1300 MEDLINE  
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[PMID]:28817121
[Au] Autor:Renfer E; Technau U
[Ad] Endereço:Department of Molecular Evolution and Development, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
[Ti] Título:Meganuclease-assisted generation of stable transgenics in the sea anemone Nematostella vectensis.
[So] Source:Nat Protoc;12(9):1844-1854, 2017 Sep.
[Is] ISSN:1750-2799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The sea anemone Nematostella vectensis is a model system used by a rapidly growing research community for comparative genomics, developmental biology and ecology. Here, we describe a microinjection procedure for creating stable transgenic lines in Nematostella based on meganuclease (I-SceI)-assisted integration of a transgenic cassette into the genome. The procedure describes the preparation of the reagents, microinjection of the transgenesis vector and the husbandry of transgenic animals. The microinjection setup differs from those of previously published protocols by the use of a holding capillary mounted on an inverted fluorescence microscope. In one session of injections, a single researcher can microinject up to 1,300 zygotes with a reporter construct digested with the meganuclease I-SceI. Under optimal conditions, fully transgenic heterozygous F1 animals can be obtained within 4-5 months of the injections, with a germ-line transmission efficiency of ∼3%. The method is versatile and, after a short training phase, can be carried out by any researcher with basic training in molecular biology. Flexibility of construct design enables this method to be used for numerous applications, including the functional dissection of cis-regulatory elements, subcellular localization of proteins, detection of protein-binding partners, ectopic expression of genes of interest, lineage tracing and cell-type-specific reporter gene expression.
[Mh] Termos MeSH primário: Animais Geneticamente Modificados/genética
Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo
Engenharia Genética/métodos
Microinjeções/métodos
Proteínas de Saccharomyces cerevisiae/metabolismo
Anêmonas-do-Mar/genética
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados/fisiologia
Clonagem Molecular
Desenho de Equipamento
Microinjeções/instrumentação
Anêmonas-do-Mar/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Saccharomyces cerevisiae Proteins); EC 3.1.21.- (SCEI protein, S cerevisiae); EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1038/nprot.2017.075


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[PMID]:28796817
[Au] Autor:Moiseeva E; Rabinowitz C; Paz G; Rinkevich B
[Ad] Endereço:Israel Oceanography and Limnological Research, National Institute of Oceanography, Tel-Shikmona, Haifa, Israel.
[Ti] Título:Histological study on maturation, fertilization and the state of gonadal region following spawning in the model sea anemone, Nematostella vectensis.
[So] Source:PLoS One;12(8):e0182677, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The starlet sea-anemone Nematostella vectensis has emerged as a model organism in developmental biology. Still, our understanding of various biological features, including reproductive biology of this model species are in its infancy. Consequently, through histological sections, we study here key stages of the oogenesis (oocyte maturation/fertilization), as the state of the gonad region immediately after natural spawning. Germ cells develop in a secluded mesenterial gastrodermal zone, where the developing oocytes are surrounded by mucoid glandular cells and trophocytes (accessory cells). During vitellogenesis, the germinal vesicle in oocytes migrates towards the animal pole and the large polarized oocytes begin to mature, characterized by karyosphere formation. Then, the karyosphere breaks down, the chromosomes form the metaphase plate I and the eggs are extruded from the animal enclosed in a sticky, jelly-like mucoid mass, along with numerous nematosomes. Fertilization occurs externally at metaphase II via swimming sperm extruded by males during natural spawning. The polar bodies are ejected from the eggs and are situated within a narrow space between the egg's vitelline membrane and the adjacent edge of the jelly coat. The cortical reaction occurs only at the polar bodies' ejection site. Several spermatozoa can penetrate the same egg. Fertilization is accompanied by a strong ooplasmatic segregation. Immediately after spawning, the gonad region holds many previtellogenic and vitellogenic oocytes, though no oocytes with karyosphere. Above are the first histological descriptions for egg maturation, meiotic chromosome's status at fertilization, fertilization and the gonadal region's state following spawning, also documenting for the first time the ejection of the polar body.
[Mh] Termos MeSH primário: Oócitos/fisiologia
Anêmonas-do-Mar/anatomia & histologia
[Mh] Termos MeSH secundário: Animais
Feminino
Fertilização
Gônadas/citologia
Masculino
Oogênese
Reprodução
Anêmonas-do-Mar/fisiologia
Interações Espermatozoide-Óvulo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182677


  9 / 1300 MEDLINE  
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[PMID]:28796463
[Au] Autor:Kim CH; Lee YJ; Go HJ; Oh HY; Lee TK; Park JB; Park NG
[Ad] Endereço:Department of Biotechnology, College of Fisheries Sciences, Pukyong National University, Busan, Korea.
[Ti] Título:Defensin-neurotoxin dyad in a basally branching metazoan sea anemone.
[So] Source:FEBS J;284(19):3320-3338, 2017 Oct.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recent studies suggest that vertebrate and invertebrate defensins have evolved from two independent ancestors, and that both defensins could share origins with animal toxins. Here, we purified novel sea anemone neurotoxin (BDS)-like antimicrobial peptides (AMPs)-Crassicorin-I and its putative homolog (Crassicorin-II)-from the pharynx extract of an anthozoan sea anemone (Urticina crassicornis). Based on structural analyses and cDNA cloning, mature Crassicorin-I represents a cationic AMP likely generated from a precursor and comprising 40 amino acid residues, including six cysteines forming three intramolecular disulfide bonds. Recombinant Crassicorin-I produced in a heterologous bacterial-expression system displayed antimicrobial activity against both a gram-positive bacterium (Bacillus subtilis) and gram-negative bacteria (Escherichia coli and Salmonella enterica). The Crassicorin-I transcript was upregulated by immune challenge, suggesting its involvement in defense mechanisms against infectious pathogens in sea anemone. Sequence alignment and three-dimensional molecular modeling revealed that Crassicorin-I exhibits high degrees of structural similarity to sea anemone neurotoxins that share ß-defensin fold which is found in vertebrate defensins and invertebrate big-defensins. Consistent with its structural similarity to neurotoxins, Crassicorin-I exhibited paralytic activity toward a crustacean. These findings motivated our investigation and subsequent discovery of antimicrobial activity from other known sea anemone neurotoxins, such as APETx1 and ShK. Collectively, our work signified that Crassicorin-I is the first AMP identified from a sea anemone and provided evidence of a functional linkage between AMPs and neurotoxins in a basally branching metazoan.
[Mh] Termos MeSH primário: Venenos de Cnidários/isolamento & purificação
Neurotoxinas/isolamento & purificação
Anêmonas-do-Mar/química
beta-Defensinas/isolamento & purificação
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Bacillus subtilis/efeitos dos fármacos
Bacillus subtilis/crescimento & desenvolvimento
Sequência de Bases
Clonagem Molecular
Venenos de Cnidários/biossíntese
Venenos de Cnidários/química
Venenos de Cnidários/toxicidade
Sequência Conservada
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Testes de Sensibilidade Microbiana
Modelos Moleculares
Neurotoxinas/biossíntese
Neurotoxinas/química
Neurotoxinas/toxicidade
Penaeidae/efeitos dos fármacos
Penaeidae/fisiologia
Peptídeos
Domínios e Motivos de Interação entre Proteínas
Estrutura Secundária de Proteína
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/química
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/toxicidade
Salmonella enterica/efeitos dos fármacos
Salmonella enterica/crescimento & desenvolvimento
Anêmonas-do-Mar/patogenicidade
Anêmonas-do-Mar/fisiologia
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
beta-Defensinas/biossíntese
beta-Defensinas/química
beta-Defensinas/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cnidarian Venoms); 0 (Neurotoxins); 0 (Peptides); 0 (Recombinant Proteins); 0 (beta-Defensins); 0 (crassicorin-I, Urticina crassicornis); 0 (crassicorin-II, Urticina crassicornis)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1111/febs.14194


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[PMID]:28710031
[Au] Autor:Chi SI; Johansen SD
[Ad] Endereço:Department of Medical Biology, Faculty of Health Sciences, UiT - The Arctic University of Norway, Tromsø, Norway.
[Ti] Título:Zoantharian mitochondrial genomes contain unique complex group I introns and highly conserved intergenic regions.
[So] Source:Gene;628:24-31, 2017 Sep 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Complex group I introns represent hallmarks of hexacoral mitochondrial genomes (mtDNAs). These intron elements are expected to influence the gene organization and gene expression. We sequenced the mitochondrial genome and transcriptome of Zoanthus sansibariscus and Palythoa heliodiscus, two zoantharian species (colonial anemones) representing different families within the suborder Brachycnemina. The circular and approximately 21kb mtDNAs contained two group I introns, one in ND5 and another in COI. The ND5-717 intron harbored two conventional mitochondrial genes (ND1 and ND3) within its structure and revealed several conserved features compared to ND5-717 in sea anemones. The COI intron, however, was inserted at a unique location (after position 867), which was different from that in sea anemones (position 884) and stony corals (position 720). COI-867 contained a homing endonuclease gene (HEG) with remarkable features, including species-specific length variations and only one copy of the essential LAGLIDADG motif. Whereas transcriptome analysis indicated that all conventional mtDNA genes were expressed, HEG expression appeared significantly repressed. Finally, we identified absolutely conserved non-coding repeat motifs with antisense features and potential regulatory functions.
[Mh] Termos MeSH primário: Sequência Conservada
DNA Mitocondrial
Genoma Mitocondrial
Íntrons
Anêmonas-do-Mar/genética
[Mh] Termos MeSH secundário: Animais
DNA Intergênico
Fases de Leitura Aberta
Análise de Sequência de DNA
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Intergenic); 0 (DNA, Mitochondrial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170716
[St] Status:MEDLINE



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