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  1 / 995 MEDLINE  
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[PMID]:29281657
[Au] Autor:Helm RR; Dunn CW
[Ad] Endereço:Brown University, Providence, RI, United States of America.
[Ti] Título:Indoles induce metamorphosis in a broad diversity of jellyfish, but not in a crown jelly (Coronatae).
[So] Source:PLoS One;12(12):e0188601, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many animals go through one or more metamorphoses during their lives, however, the molecular underpinnings of metamorphosis across diverse species are not well understood. Medusozoa (Cnidaria) is a clade of animals with complex life cycles, these life cycles can include a polyp stage that metamorphoses into a medusa (jellyfish). Medusae are produced through a variety of different developmental mechanisms-in some species polyps bud medusae (Hydrozoa), in others medusae are formed through polyp fission (Scyphozoa), while in others medusae are formed through direct transformation of the polyp (Cubozoa). To better understand the molecular mechanisms that may coordinate these different forms of metamorphosis, we tested two compounds first identified to induce metamorphosis in the moon jellyfish Aurelia aurita (indomethacin and 5-methoxy-2-methylindole) on a broad diversity of medusozoan polyps. We discovered that indole-containing compounds trigger metamorphosis across a broad diversity of species. All tested discomedusan polyps metamorphosed in the presence of both compounds, including species representatives of several major lineages within the clade (Pelagiidae, Cyaneidae, both clades of Rhizostomeae). In a cubozoan, low levels of 5-methoxy-2-methylindole reliably induced complete and healthy metamorphosis. In contrast, neither compound induced medusa metamorphosis in a coronate scyphozoan, or medusa production in either hydrozoan tested. Our results support the hypothesis that metamorphosis is mediated by a conserved induction pathway within discomedusan scyphozoans, and possibly cubozoans. However, failure of these compounds to induce metamorphosis in a coronate suggests this induction mechanism may have been lost in this clade, or is convergent between Scyphozoa and Cubozoa.
[Mh] Termos MeSH primário: Indóis/farmacologia
Metamorfose Biológica/efeitos dos fármacos
Cifozoários/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Indoles)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188601


  2 / 995 MEDLINE  
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[PMID]:29261770
[Au] Autor:Wang B; Liu D; Wang C; Wang Q; Zhang H; Liu G; He Q; Zhang L
[Ad] Endereço:Marine Bio-pharmaceutical Institute, Second Military Medical University, Shanghai, China.
[Ti] Título:Tentacle extract from the jellyfish Cyanea capillata increases proliferation and migration of human umbilical vein endothelial cells through the ERK1/2 signaling pathway.
[So] Source:PLoS One;12(12):e0189920, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wound healing is a complex biological process, and current research finds that jellyfish have a great capacity for promoting growth and healing. However, the underlying mechanisms remain unclear. Thus, this study was conducted to investigate the molecular mechanisms and effects of a tentacle extract (TE) from the jellyfish Cyanea capillata (C. capillata) on cell proliferation and migration in human umbilical vein endothelial cells (HUVECs). First, our results showed that TE at the concentration of 1 µg/ml could promote cell proliferation over various durations, induce a transition of the cells from the G1-phase to the S/G2-phase of the cell cycle, and increase the expression of cell cycle proteins (CyclinB1 and CyclinD1). Second, we found that TE could activate the PI3K/Akt, ERK1/2 and JNK MAPK signaling pathways but not the NF-κB signaling pathway or the apoptosis signaling cascade. Finally, we demonstrated that the TE-induced expression of cell cycle proteins was decreased by ERK1/2 inhibitor PD98059 but not by PI3K inhibitor LY294002 or JNK inhibitor SP600125. Similarly, the TE-enhanced migration ability of HUVECs was also markedly attenuated by PD98059. Taken together, our findings indicate that TE-induced proliferation and migration in HUVECs mainly occurred through the ERK1/2 MAPK signaling pathway. These results are instructively important for further research on the isolation and purification of growth-promoting factors from C. capillata and are hopeful as a means to improve human wound repair in unfavorable conditions.
[Mh] Termos MeSH primário: Estruturas Animais/química
Movimento Celular/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana/citologia
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Cifozoários/anatomia & histologia
Extratos de Tecidos/farmacologia
[Mh] Termos MeSH secundário: Animais
Ciclo Celular/efeitos dos fármacos
Proteínas de Ciclo Celular/metabolismo
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Ciclinas/metabolismo
Imunofluorescência
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana/metabolismo
Seres Humanos
NF-kappa B/metabolismo
Fosfatidilinositol 3-Quinases/metabolismo
Fosforilação/efeitos dos fármacos
Inibidores de Proteínas Quinases/farmacologia
Proteínas Proto-Oncogênicas c-akt/metabolismo
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Cyclins); 0 (NF-kappa B); 0 (Protein Kinase Inhibitors); 0 (Tissue Extracts); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189920


  3 / 995 MEDLINE  
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[PMID]:28777817
[Au] Autor:Dong Z; Sun T; Liang L; Wang L
[Ad] Endereço:Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong, P.R., China.
[Ti] Título:Effect of tea saponin on ephyrae and polyps of the moon jellyfish Aurelia sp.1.
[So] Source:PLoS One;12(8):e0182787, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The moon jellyfish (Aurelia sp.1) is thought to be a nuisance for the sea cucumber aquaculture, which commonly occur in the sea cucumber (Apostichopus japonicus) culture ponds of the Yellow Sea, China. To develop an appropriate method to control Aurelia sp.1 blooms, the toxic effects of tea saponin on Aurelia sp.1 ephyrae and polyps were tested in laboratory experiments. Our results revealed that tea saponin caused significant morphological changes, behavioral abnormality and mortality in Aurelia sp.1 ephyrae and polyps in 24 h and 48 h exposure experiments. The 24 h and 48 h median lethal concentrations (LC50) values of tea saponin for Aurelia sp.1 ephyrae were 1.9 and 1.1 mg L-1 respectively, while the LC50 value for Aurelia sp.1 polyps was 0.4 mg L-1 after 24h and 48 h of exposure to tea saponin. Comparison with literature results of tea saponin on A. japonicus indicates that the resistance of A. japonicus to tea saponin is 12-18 times greater than that of Aurelia sp.1 ephyrae. Therefore, the appropriate tea saponin dosage for the control of Aurelia sp.1 should be paid enough attention in order to minimize possible damage for sea cucumber. We suggest that the recommended level of tea saponin to eradicate Aurelia sp.1 ephyrae and polyps in sea cucumber culture ponds be lower than 1.35 mg L-1.
[Mh] Termos MeSH primário: Embrião não Mamífero/citologia
Desenvolvimento Embrionário/efeitos dos fármacos
Pólipos/mortalidade
Saponinas/toxicidade
Cifozoários/crescimento & desenvolvimento
Chá/toxicidade
[Mh] Termos MeSH secundário: Animais
Embrião não Mamífero/efeitos dos fármacos
Pólipos/tratamento farmacológico
Cifozoários/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Saponins); 0 (Tea)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170805
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182787


  4 / 995 MEDLINE  
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[PMID]:28614409
[Au] Autor:Hubot N; Lucas CH; Piraino S
[Ad] Endereço:Brussels Free University, Brussels, Belgium.
[Ti] Título:Environmental control of asexual reproduction and somatic growth of Aurelia spp. (Cnidaria, Scyphozoa) polyps from the Adriatic Sea.
[So] Source:PLoS One;12(6):e0178482, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Polyps of two moon jellyfish species, Aurelia coerulea and A. relicta, from two Adriatic Sea coastal habitats were incubated under multiple combinations of temperature (14, 21°C), salinity (24, 37 ppt) and food regime (9.3, 18.6, 27.9 µg C ind-1 week-1) to comparatively assess how these factors may influence major asexual reproduction processes in the two species. Both species exhibited a shared pattern of budding mode (Directly Budded Polyps: DBP; Stolonal Budded Polyps: SBP), with DBP favoured under low food supply (9.3 µg C ind -1 week-1) and low temperature (14°C), and SBP dominant under high temperature (21°C). However, A. coerulea showed an overall higher productivity than A. relicta, in terms of budding and podocyst production rates. Further, A. coerulea exhibited a wide physiological plasticity across different temperatures and salinities as typical adaptation to ecological features of transitional coastal habitats. This may support the hypothesis that the invasion of A. coerulea across coastal habitats worldwide has been driven by shellfish aquaculture, with scyphistoma polyps and resting stages commonly found on bivalve shells. On the contrary, A. relicta appears to be strongly stenovalent, with cold, marine environmental optimal preferences (salinity 37 ppt, T ranging 14-19°C), corroborating the hypothesis of endemicity within the highly peculiar habitat of the Mljet lake. By exposing A. relicta polyps to slightly higher temperature (21°C), a previously unknown developmental mode was observed, by the sessile polyp regressing into a dispersive, temporarily unattached and tentacle-less, non-feeding stage. This may allow A. relicta polyps to escape climatic anomalies associated to warming of surface layers and deepening of isotherms, by moving into deeper, colder layers. Overall, investigations on species-specific eco-physiological and ontogenetic potentials of polyp stages may contribute to clarify the biogeographic distribution of jellyfish and the phylogenetic relationships among evolutionary related sister clades.
[Mh] Termos MeSH primário: Adaptação Biológica
Reprodução Assexuada
Cifozoários/fisiologia
[Mh] Termos MeSH secundário: Animais
Ecossistema
Meio Ambiente
Oceanos e Mares
Filogenia
Cifozoários/crescimento & desenvolvimento
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178482


  5 / 995 MEDLINE  
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[PMID]:28545145
[Au] Autor:Höhn DP; Lucas CH; Thatje S
[Ad] Endereço:National Oceanography Centre Southampton, University of Southampton, Southampton, United Kingdom.
[Ti] Título:Respiratory response to temperature of three populations of Aurelia aurita polyps in northern Europe.
[So] Source:PLoS One;12(5):e0177913, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The benthic life stage (polyp or scyphistoma) of the bloom-forming jellyfish, Aurelia aurita (Linnaeus, 1759), also known as the moon jellyfish, contributes to the seasonal occurrence and abundance of medusa blooms via asexual reproduction. A. aurita is widely distributed in coastal areas in northern Europe, and one of the most studied jellyfish species. While the physiology of the visible medusa is largely understood, understanding of the physiology of the perennial benthic life-stage is scarce. To measure the physiological tolerance of A. aurita, the scyphistoma's temperature sensitivity across its distributional range was investigated. Respiration rates of polyps from three northern European locations exposed to 11 temperatures between 2 and 22°C were measured. There was a significant difference in respiration rate among the three polyp populations, which may reflect on differences in their thermal tolerance window. A critical temperature was reached at 14°C with the metabolic rate decreasing below and above that temperature. This pattern was less pronounced in the Norwegian population but polyps were able to survive, at least temporarily, those temperatures exceeding their natural range. While polyps collected from northern Norway, with a narrow environmental thermal window, displayed a low baseline metabolism with a Q10 value of 1.2, polyps from southern England and Scotland had Q10 values of 1.6 and 2.5, respectively. Differences in polyps' respiration rates across their distributional range suggest that populations have evolved adaptations to local environmental thermal conditions.
[Mh] Termos MeSH primário: Respiração
Cifozoários/fisiologia
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Animais
Europa (Continente)
Reprodução Assexuada
Cifozoários/classificação
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177913


  6 / 995 MEDLINE  
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[PMID]:28494358
[Au] Autor:Rowen DJ; Templeman MA; Kingsford MJ
[Ad] Endereço:College of Marine & Environmental Sciences, James Cook University, Townsville, QLD 4811, Australia.
[Ti] Título:Herbicide effects on the growth and photosynthetic efficiency of Cassiopea maremetens.
[So] Source:Chemosphere;182:143-148, 2017 Sep.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Herbicides from agricultural run-off have been measured in coastal systems of the Great Barrier Reef over many years. Non-target herbicide exposure, especially photosystem II herbicides has the potential to affect seagrasses and other marine species. The symbiotic benthic jellyfish Cassiopea maremetens is present in tropical/sub-tropical estuarine and marine environments. Jellyfish (n = 8 per treatment) were exposed to four separate concentrations of agricultural formulations of diuron or hexazinone to determine their sensitivity and potential for recovery to pulsed herbicide exposure. Jellyfish growth, symbiont photosynthetic activity and zooxanthellae density were analysed for herbicide-induced changes for 7 days followed by a 7 day recovery period. Both the jellyfish and endosymbiont were more sensitive to diuron than hexazinone. The 7-day EC for jellyfish growth was 0.35 µg L for Diuron and 17.5 µg L for Hexazinone respectively. Diuron exposure caused a significant decrease (p < 0.05) in jellyfish growth at 0.1 µg L , a level that is below the regional Great Barrier Reef guideline value. Jellyfish recovery was rapid with growth rates similar to control animals following removal from herbicide exposure. Both diuron and hexazinone caused significant decreases in photosynthetic efficiency (effective quantum yield) in all treatment concentrations (0.1 µg L and above) and this effect continued in the post-exposure period. As this species is frequently found in near-shore environments, they may be particularly vulnerable to herbicide run-off.
[Mh] Termos MeSH primário: Herbicidas/farmacologia
Rhizophoraceae/efeitos dos fármacos
Cifozoários/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Diurona/farmacologia
Fotossíntese/efeitos dos fármacos
Complexo de Proteína do Fotossistema II/efeitos dos fármacos
Rhizophoraceae/crescimento & desenvolvimento
Rhizophoraceae/fisiologia
Triazinas/farmacologia
Poluentes Químicos da Água/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 0 (Photosystem II Protein Complex); 0 (Triazines); 0 (Water Pollutants, Chemical); 9I3SDS92WY (Diuron); Y51727MR1Y (hexazinone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE


  7 / 995 MEDLINE  
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[PMID]:28389791
[Au] Autor:Getino-Mamet LN; Valdivia-Carrillo T; Gómez Daglio L; García-De León FJ
[Ad] Endereço:Laboratorio de Genética para la Conservación, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita, 23096, La Paz, B.C.S., México.
[Ti] Título:Isolation and characterization of 14 tetranucleotide microsatellite loci for the cannonball jellyfish (Stomolophus sp.) by next generation sequencing.
[So] Source:Mol Biol Rep;44(2):257-260, 2017 Apr.
[Is] ISSN:1573-4978
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The Cannonball jellyfish (Stomolophus sp.) is a species of jellyfish with high relevance in artisanal fishing. Studies of their populations do not extend beyond the morphological descriptions knowing that presents a great morphological variability. However, there are no genetic studies to determine the number of independent populations, so microsatellite markers become a suitable option. Since there are no species-specific microsatellite loci, in this paper, 14 new microsatellite loci are characterized. Microsatellite loci were isolated de novo through next generation sequencing, by two runs on Illumina MiSeq. A total of 506,771,269 base pair were obtained, from which 142,616 were microsatellite loci, and 1546 of them could design primers. We tested 14 primer pairs on 32 individuals from Bahía de La Paz, Gulf of California. We observed low genetic variation among loci (mean number of alleles per locus = 4.33, mean observed heterozygosity 0.381, mean expected heterozygosity 0.501). These loci are the first ones described for the species and will be helpful to carry out genetic diversity and population genetics studies.
[Mh] Termos MeSH primário: Cifozoários/genética
[Mh] Termos MeSH secundário: Alelos
Animais
Primers do DNA
Frequência do Gene/genética
Loci Gênicos/genética
Variação Genética/genética
Genética Populacional/métodos
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Repetições de Microssatélites/genética
Polimorfismo Genético/genética
Análise de Sequência de DNA
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170409
[St] Status:MEDLINE
[do] DOI:10.1007/s11033-017-4102-7


  8 / 995 MEDLINE  
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[PMID]:28388360
[Au] Autor:Tsujita N; Kuwahara H; Koyama H; Yanaka N; Arakawa K; Kuniyoshi H
[Ad] Endereço:a Graduate School of Biosphere Science , Hiroshima University , Higashi-Hiroshima , Japan.
[Ti] Título:Molecular characterization of aspartylglucosaminidase, a lysosomal hydrolase upregulated during strobilation in the moon jellyfish, Aurelia aurita.
[So] Source:Biosci Biotechnol Biochem;81(5):938-950, 2017 May.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The life cycle of the moon jellyfish, Aurelia aurita, alternates between a benthic asexual polyp stage and a planktonic sexual medusa (jellyfish) stage. Transition from polyp to medusa is called strobilation. To investigate the molecular mechanisms of strobilation, we screened for genes that are upregulated during strobilation using the differential display method and we identified aspartylglucosaminidase (AGA), which encodes a lysosomal hydrolase. Similar to AGAs from other species, Aurelia AGA possessed an N-terminal signal peptide and potential N-glycosylation sites. The genomic region of Aurelia AGA was approximately 9.8 kb in length and contained 12 exons and 11 introns. Quantitative RT-PCR analysis revealed that AGA expression increased during strobilation, and was then decreased in medusae. To inhibit AGA function, we administered the lysosomal acidification inhibitors, chloroquine or bafilomycin A1, to animals during strobilation. Both inhibitors disturbed medusa morphogenesis at the oral end, suggesting involvement of lysosomal hydrolases in strobilation.
[Mh] Termos MeSH primário: Aspartilglucosilaminase/genética
Aspartilglucosilaminase/metabolismo
Lisossomos/enzimologia
Reprodução Assexuada
Cifozoários/enzimologia
Cifozoários/fisiologia
Regulação para Cima
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Aspartilglucosilaminase/química
Sequência de Bases
Clonagem Molecular
Loci Gênicos/genética
Morfogênese
Cifozoários/genética
Cifozoários/crescimento & desenvolvimento
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.5.1.26 (Aspartylglucosylaminase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170419
[Lr] Data última revisão:
170419
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1285686


  9 / 995 MEDLINE  
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[PMID]:28258523
[Au] Autor:Frazão B; Campos A; Osório H; Thomas B; Leandro S; Teixeira A; Vasconcelos V; Antunes A
[Ad] Endereço:CIIMAR/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, s/n, 4450-208, Porto, Portugal.
[Ti] Título:Analysis of Pelagia noctiluca proteome Reveals a Red Fluorescent Protein, a Zinc Metalloproteinase and a Peroxiredoxin.
[So] Source:Protein J;36(2):77-97, 2017 Apr.
[Is] ISSN:1875-8355
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Pelagia noctiluca is the most venomous jellyfish in the Mediterranean Sea where it forms dense blooms. Although there is several published research on this species, until now none of the works has been focused on a complete protein profile of the all body constituents of this organism. Here, we have performed a detailed proteomics characterization of the major protein components expressed by P. noctiluca. With that aim, we have considered the study of jellyfish proteins involved in defense, body constituents and metabolism, and furthered explore the significance and potential application of such bioactive molecules. P. noctiluca body proteins were separated by1D SDS-PAGE and 2DE followed by characterization by nanoLC-MS/MS and MALDI-TOF/TOF techniques. Altogether, both methods revealed 68 different proteins, including a Zinc Metalloproteinase, a Red Fluorescent Protein (RFP) and a Peroxiredoxin. These three proteins were identified for the first time in P. noctiluca. Zinc Metalloproteinase was previously reported in the venom of other jellyfish species. Besides the proteins described above, the other 65 proteins found in P. noctiluca body content were identified and associated with its clinical significance. Among all the proteins identified in this work we highlight: Zinc metalloproteinase, which has a ShK toxin domain and therefore should be implicated in the sting toxicity of P. noctiluca.; the RFP which are a very important family of proteins due to its possible application as molecular markers; and last but not least the discovery of a Peroxiredoxin in this organism makes it a new natural resource of antioxidant and anti-UV radiation agents.
[Mh] Termos MeSH primário: Proteínas Luminescentes/análise
Metaloproteases/análise
Peroxirredoxinas/análise
Proteoma/análise
Cifozoários/metabolismo
[Mh] Termos MeSH secundário: Animais
Venenos de Cnidários/análise
Venenos de Cnidários/química
Eletroforese
Proteínas Luminescentes/química
Proteínas Luminescentes/metabolismo
Mar Mediterrâneo
Metaloproteases/química
Metaloproteases/metabolismo
Peroxirredoxinas/química
Peroxirredoxinas/metabolismo
Domínios Proteicos
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas em Tandem
Zinco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cnidarian Venoms); 0 (Luminescent Proteins); 0 (Proteome); 0 (ShK neurotoxin); 0 (red fluorescent protein); EC 1.11.1.15 (Peroxiredoxins); EC 3.4.- (Metalloproteases); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE
[do] DOI:10.1007/s10930-017-9695-0


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[PMID]:28158945
[Au] Autor:Ibáñez-Fonseca A; Alonso M; Arias FJ; Rodríguez-Cabello JC
[Ad] Endereço:BIOFORGE Lab, University of Valladolid - CIBER-BBN , Paseo de Belén 19, 47011 Valladolid, Spain.
[Ti] Título:Förster Resonance Energy Transfer-Paired Hydrogel Forming Silk-Elastin-Like Recombinamers by Recombinant Conjugation of Fluorescent Proteins.
[So] Source:Bioconjug Chem;28(3):828-835, 2017 Mar 15.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the last decades, recombinant structural proteins have become very promising in addressing different issues such as the lack of traceability of biomedical devices or the design of more sensitive biosensors. Among them, we find elastin-like recombinamers (ELRs), which can be designed to self-assemble into diverse structures, such as hydrogels. Furthermore, they might be combined with other protein polymers, such as silk, to give silk-elastin-like recombinamers (SELRs), holding the properties of both proteins. In this work, due to their recombinant nature, we have fused two different fluorescent proteins (FPs), i.e., the green Aequorea coerulescens enhanced green fluorescent protein and the near-infrared eqFP650, to a SELR able to form irreversible hydrogels through physical cross-linking. These recombinamers showed an emission of fluorescence similar to the single FPs, and they were capable of forming hydrogels with different stiffness (G' = 60-4000 Pa) by varying the concentration of the SELR-FPs. Moreover, the absorption spectrum of SELR-eqFP650 showed a peak greatly overlapping the emission spectrum of the SELR-Aequorea coerulescens enhanced green fluorescent protein. Hence, this enables Förster resonance energy transfer (FRET) upon the interaction between two SELR molecules, each one containing a different FP, due to the stacking of silk domains at any temperature and to the aggregation of elastin-like blocks above the transition temperature. This effect was studied by different methods, and a FRET efficiency of 0.06-0.2 was observed, depending on the technique used for its calculation. Therefore, innovative biological applications arise from the combination of SELRs with FPs, such as enhancing the traceability of hydrogels based on SELRs intended for tissue engineering, the development of biosensors, and the prediction of FRET efficiencies of novel FRET pairs.
[Mh] Termos MeSH primário: Elastina/química
Proteínas de Fluorescência Verde/química
Hidrogéis/química
Seda/química
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis
Transferência Ressonante de Energia de Fluorescência/métodos
Proteínas Recombinantes/química
Cifozoários/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Hydrogels); 0 (Recombinant Proteins); 0 (Silk); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins); 9007-58-3 (Elastin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.6b00738



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