Base de dados : MEDLINE
Pesquisa : B01.050.500.408.560.700 [Categoria DeCS]
Referências encontradas : 202 [refinar]
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  1 / 202 MEDLINE  
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[PMID]:29249162
[Au] Autor:Dong L; Li Y; Zhang D; Zhang H; Han J; Wang Z; Zhou J; Lu C; Su X
[Ad] Endereço:School of Marine Science, Ningbo University , Ningbo, China 315211.
[Ti] Título:Dietary Apostichopus japonicus Alleviates Diabetes Symptoms and Modulates Genes Expression in Kidney Tissues of db/db Mice.
[So] Source:J Agric Food Chem;66(1):154-162, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effects of Apostichopus japonicus enzymatic hydrolysate on the regulation of dyslipidemia, pathoglycemia, and transcription changes in kidney tissues of db/db mice were evaluated. In this study, the symptoms of diabetes in db/db mice were alleviated after 10 weeks of treatments with low (db/db + LD group) and high dose (db/db + HD group) of Apostichopus japonicus enzymatic hydrolysate, and the high dose treatment showed a better antidiabetic effect. Compared with the db/db group, the fasting blood glucose levels (36.84 ± 7.82 vs 25.18 ± 6.84 mmol/L, P < 0.01), the urine glucose levels (45.44 ± 3.93 vs 22.66 ± 5.58 mmol/L, P < 0.01), and the serum insulin sensitivity index (-4.65 ± 0.43 vs -4.74 ± 0.75, P > 0.05) in the db/db + HD group were decreased, whereas the fasting plasma insulin (3.12 ± 1.08 vs 5.54 ± 1.82 µg/L, P < 0.01) and the serum insulin resistance index (5.01 ± 2.02 vs 5.96 ± 2.49, P < 0.05) were increased. Subsequently, the kidney transcription profiles were measured in the db/db group and db/db + HD group via microarray, and the results show that Apostichopus japonicus hydrolysate induced differential expression of 77 genes. Among these genes, the down-regulation of genes ntrK1 and ptpN5 played vital roles, as this effect induced the further down-regulation of neurotrophin tyrosine kinase, protein tyrosine phosphatase, and other transcription factors, which are involved in the classical mitogen-activated protein kinases (MAPK) and p38MAPK signaling pathways. The inhibited MAPK and p38MAPK signaling pathways are involved in glycometabolism and the control of lipid metabolism, and they regulate the occurrence and development of diabetic nephropathy.
[Mh] Termos MeSH primário: Diabetes Mellitus Experimental/dietoterapia
Regulação da Expressão Gênica
Rim/fisiologia
Stichopus
[Mh] Termos MeSH secundário: Animais
Diabetes Mellitus Experimental/genética
Suplementos Nutricionais
Hidrólise
Insulina/sangue
Rim/fisiopatologia
Camundongos Transgênicos
Peptídeos/análise
Peptídeos/química
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Stichopus/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insulin); 0 (Peptides)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04743


  2 / 202 MEDLINE  
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[PMID]:28687360
[Au] Autor:Miao T; Wan Z; Sun L; Li X; Xing L; Bai Y; Wang F; Yang H
[Ad] Endereço:CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Qingdao 266003, China.
[Ti] Título:Extracellular matrix remodeling and matrix metalloproteinases (ajMMP-2 like and ajMMP-16 like) characterization during intestine regeneration of sea cucumber Apostichopus japonicus.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;212:12-23, 2017 Oct.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Remodeling of extracellular matrix (ECM) regulated by matrix metalloproteinases (MMPs) is essential for tissue regeneration. In the present study, we used immunohistochemistry (IHC) techniques against ECM components to reveal changes of ECM during intestine regeneration of Apostichopus japonicus. The expression of collagen I and laminin reduced apparently from the eviscerated intestine, while fibronectin exhibited continuous expression in all regeneration stages observed. Meanwhile, we cloned two MMP genes from A. japonicus by RACE PCR. The full-length cDNA of ajMMP-2 like is 2733bp and contains a predicted open reading frame (ORF) of 1716bp encoding 572 amino acids. The full-length cDNA of ajMMP-16 like is 2705bp and contains an ORF of 1452bp encoding 484 amino acids. The predicted protein sequences of each MMP contain two conserved domains, ZnMc_MMP and HX. Homology and phylogenetic analysis revealed that ajMMP-2 like and ajMMP-16 like share high sequence similarity with MMP-2 and MMP-16 from Strongylocentrotus purpuratus, respectively. Then we investigated spatio-temporal expression of ajMMP-2 like and ajMMP-16 like during different regeneration stages by qRT-PCR and IHC. The expression pattern of them showed a roughly opposite trend from that of ECM components. According to our results, a fibronectin-dominate temporary matrix is created in intestine regeneration, and it might provide structural integrity for matrix and promote cell movement. We also hypothesize that ajMMP-2 like and ajMMP-16 like could accelerate cell migration and regulate interaction between ECM components and growth factors. This work provides new evidence of ECM and MMPs involvement in sea cucumber regeneration.
[Mh] Termos MeSH primário: Intestinos/fisiologia
Regeneração/fisiologia
Stichopus/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Clonagem Molecular
Colágeno Tipo I/metabolismo
Matriz Extracelular/metabolismo
Fibronectinas/metabolismo
Regulação Enzimológica da Expressão Gênica
Imuno-Histoquímica
Laminina/metabolismo
Metaloproteinase 16 da Matriz/genética
Metaloproteinase 16 da Matriz/metabolismo
Metaloproteinase 2 da Matriz/genética
Metaloproteinase 2 da Matriz/metabolismo
Metaloproteinases da Matriz/genética
Metaloproteinases da Matriz/metabolismo
Filogenia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Regeneração/genética
Homologia de Sequência de Aminoácidos
Stichopus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Collagen Type I); 0 (Fibronectins); 0 (Laminin); 0 (RNA, Messenger); EC 3.4.24.- (Matrix Metalloproteinase 16); EC 3.4.24.- (Matrix Metalloproteinases); EC 3.4.24.24 (Matrix Metalloproteinase 2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170709
[St] Status:MEDLINE


  3 / 202 MEDLINE  
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[PMID]:28647408
[Au] Autor:Li X; Sun L; Yang H; Zhang L; Miao T; Xing L; Huo D
[Ad] Endereço:CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Mar
[Ti] Título:Identification and expression characterization of WntA during intestinal regeneration in the sea cucumber Apostichopus japonicus.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;210:55-63, 2017 Aug.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Wnt genes encode secreted glycoproteins that act as signaling molecules; these molecules direct cell proliferation, migration, differentiation and survival during animal development, maintenance of homeostasis and regeneration. At present, although the regeneration mechanism in Apostichopus japonicus has been studied, there is a little research on the Wnt signaling pathway in A. japonicus. To understand the potential role of the Wnt signaling pathway in A. japonicus, we cloned and sequenced the WntA gene in A. japonicus. Protein localization analysis showed that WntA protein was ubiquitously expressed in epidermal cells, the muscle and submucosa of the intestinal tissue. After stimulation and evisceration, the dynamic changes in expression of the WntA gene and protein showed that WntA was constitutively expressed during different stages of intestine regeneration in A. japonicus, with higher levels during the early wound healing stage and late lumen formation in the residual and nascent intestinal tissues, indicating its response to intestinal regeneration. Simultaneously, cell proliferation and apoptosis analysis showed that the patterns of cell proliferation were similar to the patterns of WntA protein expression during different intestinal regeneration stages in this organism. Taken together, these results suggested that WntA might participate in intestinal regeneration and may be connected with cell proliferation, apoptosis in different intestinal layers. This research could establish a basis for further examination of WntA functions in A. japonicus and Wnt genes in other echinoderms.
[Mh] Termos MeSH primário: Células Epiteliais/metabolismo
Intestinos/metabolismo
Regeneração/genética
Stichopus/genética
Proteínas Wnt/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Apoptose
Sequência de Bases
Proliferação Celular
Clonagem Molecular
Epiderme/citologia
Epiderme/metabolismo
Células Epiteliais/citologia
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Regulação da Expressão Gênica
Intestinos/citologia
Músculos/citologia
Músculos/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Transdução de Sinais
Stichopus/citologia
Stichopus/metabolismo
Proteínas Wnt/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins); 0 (Wnt Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170626
[St] Status:MEDLINE


  4 / 202 MEDLINE  
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[PMID]:28512032
[Au] Autor:Cuong NX; Vien LT; Hoang L; Hanh TTH; Thao DT; Thanh NV; Nam NH; Thung DC; Kiem PV; Minh CV
[Ad] Endereço:Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam; Graduate University of Science and Technology, VAST, 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
[Ti] Título:Cytotoxic triterpene diglycosides from the sea cucumber Stichopus horrens.
[So] Source:Bioorg Med Chem Lett;27(13):2939-2942, 2017 07 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Using various chromatographic separation techniques, eight triterpene diglycosides (1-8), including four new compounds namely stichorrenosides A-D (1-4), were isolated from a methanol extract of the Vietnamese sea cucumber S. horrens. Their structures were elucidated based on spectroscopic analyses, including HR ESI MS, 1D and 2D NMR. Their in vitro cytotoxic activity against five human cancer cell lines, Hep-G2 (hepatoma cancer), KB (epidermoid carcinoma), LNCaP (prostate cancer), MCF7 (breast cancer), and SK-Mel2 (melanoma), was evaluated using SRB methods. Stichorrenoside D (4), stichoposide A (5), and 3ß-O-[ß-d-xylopyranosyl-(1→2)-ß-d-xylopyranosyl]-23S-acetoxyholost-7-ene (7) showed strong cytotoxicity on all five tested cancer cell lines, whereas significant effect was observed for stichorrenoside C (3) and stichoposide B (6).
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Glicosídeos/farmacologia
Stichopus/química
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/química
Antineoplásicos/isolamento & purificação
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Glicosídeos/química
Glicosídeos/isolamento & purificação
Seres Humanos
Estrutura Molecular
Relação Estrutura-Atividade
Triterpenos/química
Triterpenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Glycosides); 0 (Triterpenes)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170518
[St] Status:MEDLINE


  5 / 202 MEDLINE  
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[PMID]:28490052
[Au] Autor:Liu YX; Zhou DY; Ma DD; Liu ZQ; Liu YF; Song L; Dong XP; Li DM; Zhu BW; Konno K; Shahidi F
[Ad] Endereço:School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
[Ti] Título:Effects of endogenous cysteine proteinases on structures of collagen fibres from dermis of sea cucumber (Stichopus japonicus).
[So] Source:Food Chem;232:10-18, 2017 Oct 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Autolysis of sea cucumber, caused by endogenous enzymes, leads to postharvest quality deterioration of sea cucumber. However, the effects of endogenous proteinases on structures of collagen fibres, the major biologically relevant substrates in the body wall of sea cucumber, are less clear. Collagen fibres were prepared from the dermis of sea cucumber (Stichopus japonicus), and the structural consequences of degradation of the collagen fibres caused by endogenous cysteine proteinases (ECP) from Stichopus japonicus were examined. Scanning electron microscopic images showed that ECP caused partial disaggregation of collagen fibres into collagen fibrils by disrupting interfibrillar proteoglycan bridges. Differential scanning calorimetry and Fourier transform infrared analysis revealed increased structural disorder of fibrillar collagen caused by ECP. SDS-PAGE and chemical analysis indicated that ECP can liberate glycosaminoglycan, hydroxyproline and collagen fragments from collagen fibres. Thus ECP can cause disintegration of collagen fibres by degrading interfibrillar proteoglycan bridges.
[Mh] Termos MeSH primário: Colágeno/química
Cisteína Proteases
Derme/química
Pepinos-do-Mar/enzimologia
[Mh] Termos MeSH secundário: Animais
Derme/citologia
Pepinos-do-Mar/química
Pepinos-do-Mar/citologia
Stichopus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-34-5 (Collagen); EC 3.4.- (Cysteine Proteases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE


  6 / 202 MEDLINE  
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[PMID]:28369350
[Au] Autor:Jo J; Oh J; Lee HG; Hong HH; Lee SG; Cheon S; Kern EMA; Jin S; Cho SJ; Park JK; Park C
[Ad] Endereço:School of Biological Sciences and Technology, Chonnam National University, Gwangju 61186, Republic of Korea.
[Ti] Título:Draft genome of the sea cucumber Apostichopus japonicus and genetic polymorphism among color variants.
[So] Source:Gigascience;6(1):1-6, 2017 Jan 01.
[Is] ISSN:2047-217X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Japanese sea cucumber (Apostichopus japonicus Selenka 1867) is an economically important species as a source of seafood and ingredient in traditional medicine. It is mainly found off the coasts of northeast Asia. Recently, substantial exploitation and widespread biotic diseases in A. japonicus have generated increasing conservation concern. However, the genomic knowledge base and resources available for researchers to use in managing this natural resource and to establish genetically based breeding systems for sea cucumber aquaculture are still in a nascent stage. A total of 312 Gb of raw sequences were generated using the Illumina HiSeq 2000 platform and assembled to a final size of 0.66 Gb, which is about 80.5% of the estimated genome size (0.82 Gb). We observed nucleotide-level heterozygosity within the assembled genome to be 0.986%. The resulting draft genome assembly comprising 132 607 scaffolds with an N50 value of 10.5 kb contains a total of 21 771 predicted protein-coding genes. We identified 6.6-14.5 million heterozygous single nucleotide polymorphisms in the assembled genome of the three natural color variants (green, red, and black), resulting in an estimated nucleotide diversity of 0.00146. We report the first draft genome of A. japonicus and provide a general overview of the genetic variation in the three major color variants of A. japonicus. These data will help provide a comprehensive view of the genetic, physiological, and evolutionary relationships among color variants in A. japonicus, and will be invaluable resources for sea cucumber genomic research.
[Mh] Termos MeSH primário: Genes
Genoma
Polimorfismo de Nucleotídeo Único
Análise de Sequência de DNA
Stichopus/genética
[Mh] Termos MeSH secundário: Animais
Cor
Genômica
Masculino
Pigmentação/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170810
[Lr] Data última revisão:
170810
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1093/gigascience/giw006


  7 / 202 MEDLINE  
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[PMID]:28284150
[Au] Autor:Charan-Dixon H; Gaw S; Goldstien SJ; Glover CN
[Ad] Endereço:School of Biological Sciences, University of Canterbury, New Zealand.
[Ti] Título:Effects of waterborne cadmium on energy metabolism in the tropical sea cucumber, Stichopus horrens, and a comparison of tissue-specific cadmium accumulation with the temperate sea cucumber Australostichopus mollis.
[So] Source:Ecotoxicol Environ Saf;141:1-8, 2017 Jul.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The sea cucumber Stichopus horrens is an important component of near-shore ecosystems, and in the Kingdom of Tonga it also comprises an important commercial and subsistence fishery. To assess the sensitivity of this species to the toxic trace metal cadmium (Cd), adult S. horrens were exposed for 96h to an environmental (15µgL ) or effect (765µgL ) concentration of waterborne Cd. The respiratory tree and intestine accumulated higher concentrations of Cd than the muscle and body wall, but there were no effects of Cd on tissue ions (sodium, potassium, magnesium, calcium). For comparison, Cd accumulation was also examined in the Australasian sea cucumber Australostichopus mollis. This species displayed a similar pattern of tissue-specific accumulation to S. horrens, but exhibited lower tissue Cd burdens, likely a consequence of lower experimental temperature. Effects on gonad ion content were also seen in this species. At the highest Cd exposure concentration, S. horrens showed impaired ammonia excretion rates and an increased molar oxygen:nitrogen ratio (O:N), indicative of a decreased reliance on protein metabolism. Overall, this study suggests that S. horrens is relatively tolerant of Cd exposure, but raises concerns regarding the subsistence fishery practice of consuming the viscera of this species.
[Mh] Termos MeSH primário: Cádmio/toxicidade
Metabolismo Energético/efeitos dos fármacos
Stichopus/efeitos dos fármacos
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Amônia/metabolismo
Animais
Cádmio/metabolismo
Stichopus/metabolismo
Poluentes Químicos da Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); 00BH33GNGH (Cadmium); 7664-41-7 (Ammonia)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170312
[St] Status:MEDLINE


  8 / 202 MEDLINE  
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[PMID]:28254498
[Au] Autor:Ma S; Sun Y; Wang F; Mi R; Wen Z; Li X; Meng N; Li Y; Du X; Li S
[Ad] Endereço:Dalian Biotechnology Research Institute, Liaoning Academy of Agricultural Sciences, Dalian 116024, PR China.
[Ti] Título:Effects of tussah immunoreactive substances on growth, immunity, disease resistance against Vibrio splendidus and gut microbiota profile of Apostichopus japonicus.
[So] Source:Fish Shellfish Immunol;63:471-479, 2017 Apr.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tussah immunoreactive substance (TIS) comprises a number of active chemicals with various bioactivities. The current study investigated the effects of these substances on the sea cucumber Apostichopus japonicus. The specific growth rate (SGR) of TIS-fed sea cucumbers was significantly enhanced, whereas no significant difference in SGR was observed between those soaked in antibiotics and those fed with basal diet only. TIS also improved the immune response of the animals when given at a dose of 1.0% or 2.0%, as shown by increased phagocytic, lysozyme, superoxide dismutase, alkaline phosphatase, acid phosphatase, and catalase activities following injection with live Vibrio splendidus. At a dose of 1.0% or 2.0%, TIS significantly enhanced the immune ability (P < 0.05) of the sea cucumbers, but except for lysozyme activity, other immune indices were reduced one day after the animals were injected with Vibrio splendidus. However, the values of these immune indexes were still significantly higher compared to those of the control groups (P < 0.05). Intestinal micro flora counts and high-throughput sequencing showed that dietary TIS could improve the amount of probiotic bacteria, yielding a 6-fold increase in Bacillus and 10-fold increase in Lactobacillus for sea cucumbers fed with 2.0% TIS diet compared to the control. Furthermore, TIS-containing diet also greatly reduced the number of harmful bacteria, with the number of Vibrio in sea cucumbers fed with 1%TIS diet decreased by 67% compared to the control. The results thus indicated that TIS increased the growth of sea cucumbers and enhanced their resistance to V. splendidus infection by improving the immunity of the animals. TIS also improved the gut microbiota profiles of the animals by increasing the probiotics and reducing the harmful bacteria within their guts.
[Mh] Termos MeSH primário: Suplementos Nutricionais
Microbioma Gastrointestinal/fisiologia
Imunidade Inata
Mariposas/química
Stichopus/microbiologia
Stichopus/fisiologia
Vibrio/fisiologia
[Mh] Termos MeSH secundário: Ração Animal/análise
Animais
Dieta
Relação Dose-Resposta a Droga
Mariposas/crescimento & desenvolvimento
Probióticos/análise
Pupa/química
Distribuição Aleatória
Stichopus/crescimento & desenvolvimento
Stichopus/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE


  9 / 202 MEDLINE  
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[PMID]:28238861
[Au] Autor:Jiang J; Zhou Z; Dong Y; Gao S; Sun H; Chen Z; Yang A; Su H
[Ad] Endereço:Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning 116023, PR China.
[Ti] Título:Comparative analysis of immunocompetence between females and males in the sea cucumber Apostichopus japonicus.
[So] Source:Fish Shellfish Immunol;63:438-443, 2017 Apr.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In order to preliminarily understand the immune difference between females and males in the sea cucumber Apostichopus japonicus, the activities assay of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), phenoloxidase (PO), acid phosphatase (ACP) and alkaline phosphatase (ALP) with biochemical methods, the detection of PO isozymes with native-PAGE and catechol staining, and the test of antibacterial activities with bacterial growth curve determination method were performed in this study using cell-free coelomic fluid (CCF) and coelomocyte lysate supernatant (CLS) from females and males as the samples. The PO activities were not detected in the CLS and showed no significant difference between the CCF from females and males. However, totally five PO isozyme bands were detected in the CLS of females while only four were detected in the CLS of males after zymogram analysis. These results implied that the PO isozymes in the coelomocytes of viripotent A. japonicus were inactive under natural condition and may be activated by some certain treatments during native-PAGE, and PO might play different immune and physiological roles between females and males. In addition, the activities of SOD, CAT, POD and ALP in the CCF and the activities of CAT, POD, ACP and ALP in the CLS from males were all significantly higher than those from females. The results collectively suggested that in viripotent A. japonicus, the gender had a remarkable effect on the immunity, and the immunocompetence of males might have an advantage over that of females. Furthermore, the activities of all determined enzymes except PO and the number of detected PO isozymes showed higher values in CLS than in CCF, implying that in viripotent A. japonicus, the coelomocytes might take more immune responsibility in comparison with CCF.
[Mh] Termos MeSH primário: Imunocompetência
Micrococcus/fisiologia
Stichopus/imunologia
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Fatores Sexuais
Stichopus/enzimologia
Stichopus/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE


  10 / 202 MEDLINE  
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[PMID]:28185912
[Au] Autor:Shao Y; Lv Z; Li C; Zhang W; Duan X; Qiu Q; Jin C; Zhao X
[Ad] Endereço:School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China.
[Ti] Título:Molecular cloning and functional characterization of theta class glutathione S-transferase from Apostichopus japonicus.
[So] Source:Fish Shellfish Immunol;63:31-39, 2017 Apr.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Glutathione S-transferases (GSTs) are the superfamily of multifunctional detoxification isoenzymes and play crucial roles in innate immunity. In the present study, a theta class GST homology was identified from A. japonicus (designated as AjGST-θ) by RACE approaches. The full-length cDNA of AjGST-θ was of 1013 bp encoded a cytosolic protein of 231 amino acids residues. Structural analysis revealed that AjGST-θ processed the characteristic N-terminal GSH-binding site (G-site) and the C-terminal hydrophobic substrate binding site (H-site). Multiple sequence alignment and phylogenetic analysis together supported that AjGST-θ belonged to a new member of theta class GST protein subfamily. Spatial expression analysis revealed that AjGST-θ was ubiquitously expressed in all examined tissues with the larger magnitude in intestine. The Vibrio splendidus challenge in vivo and LPS stimulation in vitro could both significantly up-regulate the mRNA expression of AjGST-θ when compared with control group. The recombinant protein was expressed in Escherichia coli and the purified AjGST-θ showed high activity with GST substrate. Meantime, disc diffusion assay showed that recombinant AjGST-θ protein could markedly improve bacterial growth under Cumene hydroperoxide exposure. More importantly, the recombinant AjGST-θ could effectively prevent primary coelomocytes apoptosis after LPS exposure. Our present findings suggested that AjGST-θ might play significantly roles in the modulation of immune response and protect cells from pathogens infection in A. japonicus.
[Mh] Termos MeSH primário: Glutationa Transferase/genética
Imunidade Inata
Stichopus/enzimologia
Stichopus/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Apoptose
Sequência de Bases
Clonagem Molecular
DNA Complementar/genética
DNA Complementar/metabolismo
Escherichia coli/genética
Glutationa Transferase/química
Glutationa Transferase/metabolismo
Lipopolissacarídeos/farmacologia
Filogenia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Stichopus/classificação
Stichopus/microbiologia
Vibrio/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Lipopolysaccharides); 0 (RNA, Messenger); 0 (Recombinant Proteins); EC 2.5.1.18 (Glutathione Transferase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE



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