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Pesquisa : B01.050.500.408.578.050 [Categoria DeCS]
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  1 / 19 MEDLINE  
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[PMID]:27959777
[Au] Autor:Lee JY; Kim PS; Hyun DW; Kim HS; Shin NR; Jung MJ; Yun JH; Kim MS; Whon TW; Bae JW
[Ad] Endereço:Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 02447, Republic of Korea.
[Ti] Título:Microbulbifer echini sp. nov., isolated from the gastrointestinal tract of a purple sea urchin, Heliocidaris crassispina.
[So] Source:Int J Syst Evol Microbiol;67(4):998-1004, 2017 Apr.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel bacterium, designated as strain AM134T, was isolated from the gut of a purple sea urchin (Heliocidaris crassispina) gathered from the coastal waters of Dokdo, Korea. Strain AM134T was Gram-stain-negative, both catalase- and oxidase-positive, strictly aerobic and showed a rod-coccus cell cycle. Optimum growth occurred at 30 °C, in the presence of 2 % (w/v) NaCl and at pH 7. The 16S rRNA gene sequence analysis showed that strain AM134T belonged to the genus Microbulbifer in the family Alteromonadaceae and had high 16S rRNA gene sequence similarity (>97 %) with Microbulbifer epialgicus F-104T (98.9 % similarity) and Microbulbifer variabilis Ni-2088T (98.6 % similarity). The polar lipid profile of strain AM134T was composed of phosphatidylethanolamine, phosphatidylserine, three unidentified aminophospholipids, two unidentified phospholipids, an unidentified amino lipid and six unidentified lipids. The major respiratory quinone was identified as ubiquinone-8 (Q-8). The major cellular fatty acids were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) and C16 : 0. The DNA-DNA hybridization analysis showed that the strain shared less than 28 % genomic relatedness with Microbulbifer epialgicus DSM 18651T (27±3 %) and Microbulbifer variabilis ATCC 700307T (15±1 %). The G+C content of the genomic DNA was 56.1 mol%. The results of the phylogenetic, phenotypic and genotypic analyses suggest that strain AM134T represents a novel species in the genus Microbulbifer, for which the name Microbulbifer echini is proposed. The type strain is AM134T (=KACC 18258T=JCM 30400T).
[Mh] Termos MeSH primário: Alteromonadaceae/classificação
Anthocidaris/microbiologia
Trato Gastrointestinal/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Alteromonadaceae/genética
Alteromonadaceae/isolamento & purificação
Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001731


  2 / 19 MEDLINE  
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[PMID]:26868361
[Au] Autor:Lee JY; Hyun DW; Soo Kim P; Sik Kim H; Shin NR; Yun JH; Jung MJ; Kim MS; Woong Whon T; Bae JW
[Ad] Endereço:Department of Biology and Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Korea.
[Ti] Título:Arthrobacter echini sp. nov., isolated from the gut of a purple sea urchin, Heliocidaris crassispina.
[So] Source:Int J Syst Evol Microbiol;66(4):1887-93, 2016 Apr.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel strain, designated AM23T, was isolated from the gut of a purple sea urchin Heliocidaris crassispina collected from the coastal waters of the Korean island Dokdo. 16S rRNA gene sequence analysis showed that strain AM23T belonged to the genus Arthrobacter in the family Micrococcaceae and shared highest sequence similarity with Arthrobacter agilis DSM 20550T (98.77%). Strain AM23T was catalase-positive, oxidase-negative and grew optimally at 20 °C, in the presence of 1% (w/v) NaCl and at pH 7. The isolate was a Gram-stain-positive, non-motile, strictly aerobic and coccus-shaped bacterium. The major cellular fatty acids were anteiso-C15:0 and iso-C15:0. The polar lipids of strain AM23T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid and four unidentified lipids. The components of the cell-wall peptidoglycan were lysine, glutamic acid and alanine and the predominant cell-wall sugars were galactose, mannose, rhamnose and ribose. The major respiratory quinone was identified as menaquinone MK-9(H2). The genomic DNA G+C content was 67.3 mol% and the DNA-DNA hybridization values showed the strain shared less than 29% genomic relatedness with A. agilis DSM 20550T. The results of the phylogenetic, phenotypic and genotypic analysis indicate that strain AM23T represents a novel species in the genus Arthrobacter, for which the name Arthrobacter echini sp. nov. is proposed. The type strain is AM23T (=KACC 18260T=DSM 29493T).
[Mh] Termos MeSH primário: Anthocidaris/microbiologia
Arthrobacter/classificação
Trato Gastrointestinal/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Animais
Arthrobacter/genética
Arthrobacter/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Glicolipídeos/química
Dados de Sequência Molecular
Hibridização de Ácido Nucleico
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160421
[Lr] Data última revisão:
160421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160213
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.000965


  3 / 19 MEDLINE  
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[PMID]:26066611
[Au] Autor:Byrne M; Koop D; Cisternas P; Strbenac D; Yang JY; Wray GA
[Ad] Endereço:Schools of Medical and Biological Sciences, The University of Sydney, Sydney, NSW 2006, Australia.
[Ti] Título:Transcriptomic analysis of Nodal- and BMP-associated genes during juvenile development of the sea urchin Heliocidaris erythrogramma.
[So] Source:Mar Genomics;24 Pt 1:41-5, 2015 Dec.
[Is] ISSN:1876-7478
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Understanding the unusual radial body plan of echinoderms and its relationship to the bilateral plan of other deuterostomes remains a challenge. The molecular processes of embryonic and early larval development in sea urchins are well characterised, but those giving rise to the adult and its radial body remain poorly studied. We used the developmental transcriptome generated for Heliocidaris erythrogramma, a species that forms the juvenile soon after gastrulation, to investigate changes in gene expression underlying radial body development. As coelomogenesis is key to the development of pentamery and juvenile formation on the left side of the larva, we focussed on genes associated with the nodal and BMP2/4 network that pattern this asymmetry. We identified 46 genes associated with this Nodal and BMP2/4 signalling network, and determined their expression profiles from the gastrula, through to rudiment development, metamorphosis and the fully formed juvenile. Genes associated with Nodal signalling shared similar expression profiles, indicating that they may have a regulatory relationship in patterning morphogenesis of the juvenile sea urchin. Similarly, many genes associated with BMP2/4 signalling had similar expression profiles through juvenile development. Further examination of the roles of Nodal- and BMP2/4-associated genes is required to determine function and whether the gene expression profiles seen in H. erythrogramma are due to ongoing activity of gene networks established during early development, or to redeployment of regulatory cassettes to pattern the adult radial body plan.
[Mh] Termos MeSH primário: Anthocidaris/genética
Proteínas Morfogenéticas Ósseas/metabolismo
Regulação da Expressão Gênica no Desenvolvimento/fisiologia
Proteína Nodal/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Anthocidaris/crescimento & desenvolvimento
Proteínas Morfogenéticas Ósseas/genética
Proteína Nodal/genética
Transdução de Sinais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bone Morphogenetic Proteins); 0 (Nodal Protein)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151215
[Lr] Data última revisão:
151215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150613
[St] Status:MEDLINE


  4 / 19 MEDLINE  
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[PMID]:25115741
[Au] Autor:Hardy NA; Byrne M
[Ad] Endereço:School of Medical Sciences, University of Sydney, NSW 2006, Australia. Electronic address: natasha.hardy@sydney.edu.au.
[Ti] Título:Early development of congeneric sea urchins (Heliocidaris) with contrasting life history modes in a warming and high CO2 ocean.
[So] Source:Mar Environ Res;102:78-87, 2014 Dec.
[Is] ISSN:1879-0291
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The impacts of ocean change stressors - warming and acidification - on marine invertebrate development have emerged as a significant impact of global change. We investigated the response of early development to the larval stage in sympatric, congeneric sea urchins, Heliocidaris tuberculata and Heliocidaris erythrogramma with contrasting modes of development to ocean warming and acidification. Effects of these stressors were assessed by quantifying the percentage of normal development during the first 24 h post fertilization, in cross-factorial experiments that included three temperature treatments (control: 20 °C; +4: 24 °C; +6: 26 °C) and four pHNIST levels (control: 8.2; -0.4: 7.8; -0.6: 7.6; -0.8: 0.4). The experimental treatments were designed in context with present day and near-future (∼2100) conditions for the southeast Australia global warming hotspot. Temperature was the most important factor affecting development of both species causing faster progression through developmental stages as well as a decrease in the percentage of normal development. H. erythrogramma embryos were less tolerant of increased temperature than those of H. tuberculata. Acidification impaired development to the larval stage in H. tuberculata, but this was not the case for H. erythrogramma. Thus, outcomes for the planktonic life phase of the two Heliocidaris species in response to ocean warming and acidification will differ. As shown for these species, single-stressor temperature or acidification studies can be misleading with respect to determining species' vulnerability and responses to global change.
[Mh] Termos MeSH primário: Anthocidaris/embriologia
Anthocidaris/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Animais
Anthocidaris/fisiologia
Austrália
Dióxido de Carbono
Embrião não Mamífero
Aquecimento Global
Larva/crescimento & desenvolvimento
Estágios do Ciclo de Vida
Oceanos e Mares
Água do Mar/química
Especificidade da Espécie
Temperatura Ambiente
Zooplâncton
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:141202
[Lr] Data última revisão:
141202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140814
[St] Status:MEDLINE


  5 / 19 MEDLINE  
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[PMID]:24916139
[Au] Autor:Choi JY; Habte G; Khan N; Nho EY; Hong JH; Choi H; Park KS; Kim KS
[Ad] Endereço:a Department of Food and Nutrition , Chosun University , Gwangju , Republic of Korea.
[Ti] Título:Determination of toxic heavy metals in Echinodermata and Chordata species from South Korea.
[So] Source:Food Addit Contam Part B Surveill;7(4):295-301, 2014.
[Is] ISSN:1939-3229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study aimed at analysing concentrations of heavy metals including arsenic, lead, cadmium, aluminium and mercury in commonly consumed seafood species belonging to Echinodermata (Anthocidaris crassispina and Stichopus japonicus) and Chordata (Halocynthia roretzi and Styela plicata). The samples were digested by a microwave system and analysed for As, Cd and Pb by inductively coupled plasma mass spectrometer, for Al by inductively coupled plasma-optical emission spectrometer and Hg by Direct Mercury Analyser. The analytical method was validated by determining sensitivity, linearity, precision, spiking recoveries and analysis of the Standard Reference Material (SRM) NIST 1566-b, an Oyster Tissue. Results showed considerably higher accumulation of Al and As in analysed samples, compared to Pb and Cd, while Hg had the lowest contamination. On comparison, the obtained results with the recommended standards by the Food and Agriculture Organization, European Commission and Ministry of Food and Drug Safety of Korea, it was concluded that the analysed seafoods were safe and thus would not pose a threat to consumers.
[Mh] Termos MeSH primário: Cordados
Equinodermos
Contaminação de Alimentos/análise
Metais Pesados/análise
Alimentos Marinhos/análise
[Mh] Termos MeSH secundário: Alumínio/análise
Animais
Anthocidaris/química
Arsênico/análise
Cádmio/análise
Segurança de Produtos ao Consumidor
Seres Humanos
Chumbo/análise
República da Coreia
Stichopus/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Metals, Heavy); 00BH33GNGH (Cadmium); 2P299V784P (Lead); CPD4NFA903 (Aluminum); N712M78A8G (Arsenic)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:160518
[Lr] Data última revisão:
160518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140612
[St] Status:MEDLINE
[do] DOI:10.1080/19393210.2014.932311


  6 / 19 MEDLINE  
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[PMID]:23980665
[Au] Autor:Lymbery RA; Evans JP
[Ad] Endereço:Centre for Evolutionary Biology, School of Animal Biology, University of Western Australia, Crawley, 6009, WA, Australia.
[Ti] Título:Genetic variation underlies temperature tolerance of embryos in the sea urchin Heliocidaris erythrogramma armigera.
[So] Source:J Evol Biol;26(10):2271-82, 2013 Oct.
[Is] ISSN:1420-9101
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Ocean warming can alter natural selection on marine systems, and in many cases, the long-term persistence of affected populations will depend on genetic adaptation. In this study, we assess the potential for adaptation in the sea urchin Heliocidaris erythrogramma armigera, an Australian endemic, that is experiencing unprecedented increases in ocean temperatures. We used a factorial breeding design to assess the level of heritable variation in larval hatching success at two temperatures. Fertilized eggs from each full-sibling family were tested at 22 °C (current spawning temperature) and 25 °C (upper limit of predicted warming this century). Hatching success was significantly lower at higher temperatures, confirming that ocean warming is likely to exert selection on this life-history stage. Our analyses revealed significant additive genetic variance and genotype-by-environment interactions underlying hatching success. Consistent with prior work, we detected significant nonadditive (sire-by-dam) variance in hatching success, but additionally found that these interactions were modified by temperature. Although these findings suggest the potential for genetic adaptation, any evolutionary responses are likely to be influenced (and possibly constrained) by complex genotype-by-environment and sire-by-dam interactions and will additionally depend on patterns of genetic covariation with other fitness traits.
[Mh] Termos MeSH primário: Anthocidaris/embriologia
Mudança Climática
Embrião não Mamífero/fisiologia
Temperatura Ambiente
[Mh] Termos MeSH secundário: Adaptação Biológica/genética
Animais
Anthocidaris/genética
Anthocidaris/fisiologia
Desenvolvimento Embrionário/genética
Feminino
Fertilização
Masculino
Fenótipo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1404
[Cu] Atualização por classe:130913
[Lr] Data última revisão:
130913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130829
[St] Status:MEDLINE
[do] DOI:10.1111/jeb.12225


  7 / 19 MEDLINE  
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[PMID]:21791161
[Au] Autor:Reunov AA
[Ad] Endereço:Department of Embryology, A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of Russian Academy of Sciences, 17 Paltchevsky Street, Vladivostok 600041, Russia. arkadiy_reunov@hotmail.com
[Ti] Título:Premeiotic transformation of germ plasm-related structures during the sea urchin spermatogenesis.
[So] Source:Zygote;21(1):95-101, 2013 Feb.
[Is] ISSN:1469-8730
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The germ plasm-related structures (GPRS) and the transformation that occurs to them during the spermatogenesis of the sea urchin Anthocidaris crassispina were studied by electron microscopy and morphometry. The GPRS were observed in spermatogonia and spermatocytes, but not in spermatids and sperm, which suggests an important role for these structures during the onset of meiosis. It was proposed that the germinal granules are fragmented into the compact electron-dense nuage, and fragments of the latter penetrate into the periphery of the compact electron-lucent nuage. The process of nuage integration is completed with the formation of the combined nuage, which aggregates some mitochondria into clusters. Once formed, the mitochondrial clusters undergo dissemination and assume the appearance of the dispersed nuage with mitochondrial derivatives, which in turn develops into the scattered nuage. The scattered nuage, which presumably presents the composite mixture saturated with mitochondrial matrix, terminates the GPRS transformation.
[Mh] Termos MeSH primário: Anthocidaris/fisiologia
Espermatogênese/fisiologia
Espermatozoides/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Masculino
Mitocôndrias/ultraestrutura
Espermátides/ultraestrutura
Espermatócitos/ultraestrutura
Espermatogônias/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1309
[Cu] Atualização por classe:130122
[Lr] Data última revisão:
130122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110728
[St] Status:MEDLINE
[do] DOI:10.1017/S0967199411000402


  8 / 19 MEDLINE  
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[PMID]:23106717
[Au] Autor:Binks RM; Prince J; Evans JP; Kennington WJ
[Ad] Endereço:School of Animal Biology, The University of Western Australia, Crawley, Western Australia 6009, Australia. rachel.binks@grs.uwa.edu.au
[Ti] Título:More than bindin divergence: reproductive isolation between sympatric subspecies of a sea urchin by asynchronous spawning.
[So] Source:Evolution;66(11):3545-57, 2012 Nov.
[Is] ISSN:1558-5646
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The evolution of reproductive barriers is crucial to the process of speciation. In the Echinoidea, studies have focused on divergence in the gamete recognition protein, bindin, as the primary isolating mechanism among species. As such, the capacity of alternate mechanisms to be effective reproductive barriers and the phylogenetic context in which they arise is unclear. Here, we examine the evolutionary histories and factors limiting gene exchange between two subspecies of Heliocidaris erythrogramma that occur sympatrically in Western Australia. We found low, but significant differentiation between the subspecies in two mitochondrial genes. Further, coalescent analyses suggest that they diverged in isolation on the east and west coasts of Australia, with a subsequent range expansion of H. e. erythrogramma into Western Australia. Differentiation in bindin was minimal, indicating gamete incompatibility is an unlikely reproductive barrier. We did, however, detect strong asynchrony in spawning seasons; H. e. erythrogramma spawned over summer whereas H. e. armigera spawned in autumn. Taken together, we provide compelling evidence for a recent divergence of these subspecies and their reproductive isolation without gamete incompatibility. Western Australian H. erythrogramma may therefore present an intriguing case of incipient speciation, which depends on long-term persistence of the factors underlying this spawning asynchrony.
[Mh] Termos MeSH primário: Anthocidaris/fisiologia
Receptores de Superfície Celular/genética
Isolamento Reprodutivo
[Mh] Termos MeSH secundário: Animais
Anthocidaris/genética
DNA Mitocondrial/genética
Complexo IV da Cadeia de Transporte de Elétrons/genética
Feminino
Masculino
Repetições de Microssatélites
Dados de Sequência Molecular
Filogenia
RNA Ribossômico 16S/genética
Reprodução
Alinhamento de Sequência
Análise de Sequência de DNA
Simpatria
Fatores de Tempo
Austrália Ocidental
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (RNA, Ribosomal, 16S); 0 (Receptors, Cell Surface); 0 (egg surface sperm receptor); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:121030
[Lr] Data última revisão:
121030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121031
[St] Status:MEDLINE
[do] DOI:10.1111/j.1558-5646.2012.01700.x


  9 / 19 MEDLINE  
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[PMID]:23016124
[Au] Autor:Peng J; Yuan JP; Wang JH
[Ad] Endereço:Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510275, China. pengj28@mail.sysu.edu.cn
[Ti] Título:Effect of diets supplemented with different sources of astaxanthin on the gonad of the sea urchin Anthocidaris crassispina.
[So] Source:Nutrients;4(8):922-34, 2012 08.
[Is] ISSN:2072-6643
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The effect of the microalgae Haematococcus pluvialis and Chorella zofingiensis, and synthetic astaxanthin on the gonad of the sea urchin Anthocidaris crassispina was studied. The basal diet was supplemented with H. pluvialis, C. zofingiensis, or synthetic astaxanthin, at two levels of astaxanthin (approximately 400 mg/kg and 100 mg/kg), to obtain the experimental diets HP1, HP2, CZ1, CZ2, AST1, and AST2, respectively, for two months of feeding experiment. The results showed that the concentrations of astaxanthin in the gonads of the sea urchins fed these experimental diets ranged from 0.15 to 3.01 mg/kg dry gonad weight. The higher astaxanthin levels (>2.90 mg/kg) were found in the gonads of the sea urchins fed the diets HP1 (containing 380 mg/kg of astaxanthins, mostly mono- and diesters) and AST1 (containing 385 mg/kg of synthetic astaxanthin). The lowest astaxanthin level (0.15 mg/kg) was detected in the gonads of the sea urchins fed the diet CZ2 (containing 98 mg/kg of astaxanthins, mostly diesters). Furthermore, the highest canthaxanthin level (7.48 mg/kg) was found in the gonads of the sea urchins fed the diet CZ1 (containing 387 mg/kg of astaxanthins and 142 mg/kg of canthaxanthin), suggesting that astaxanthins, especially astaxanthin esters, might not be assimilated as easily as canthaxanthin by the sea urchins. Our results show that sea urchins fed diets containing astaxanthin pigments show higher incorporation of these known antioxidant constituents, with the resultant seafood products therefore being of potential higher nutritive value.
[Mh] Termos MeSH primário: Ração Animal/análise
Anthocidaris/fisiologia
Gônadas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Fenômenos Fisiológicos da Nutrição Animal
Animais
Aquicultura
Dieta
Suplementos Nutricionais
Xantofilas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Xanthophylls); 8XPW32PR7I (astaxanthine)
[Em] Mês de entrada:1302
[Cu] Atualização por classe:170510
[Lr] Data última revisão:
170510
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120928
[St] Status:MEDLINE


  10 / 19 MEDLINE  
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[PMID]:22446733
[Au] Autor:Dheilly NM; Haynes PA; Raftos DA; Nair SV
[Ad] Endereço:Department of Biological Sciences, Macquarie University, North Ryde, NSW 2109, Australia.
[Ti] Título:Time course proteomic profiling of cellular responses to immunological challenge in the sea urchin, Heliocidaris erythrogramma.
[So] Source:Dev Comp Immunol;37(2):243-56, 2012 Jun.
[Is] ISSN:1879-0089
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Genome sequences and high diversity cDNA arrays have provided a detailed molecular understanding of immune responses in a number of invertebrates, including sea urchins. However, complementary analyses have not been undertaken at the level of proteins. Here, we use shotgun proteomics to describe changes in the abundance of proteins from coelomocytes of sea urchins after immunological challenge and wounding. The relative abundance of 345 reproducibly identified proteins were measured 6, 24 and 48 h after injection. Significant changes in the relative abundance of 188 proteins were detected. These included pathogen-binding proteins, such as the complement component C3 and scavenger receptor cysteine rich proteins, as well as proteins responsible for cytoskeletal remodeling, endocytosis and intracellular signaling. An initial systemic reaction to wounding was followed by a more specific response to immunological challenge involving proteins such as apolipophorin, dual oxidase, fibrocystin L, aminopeptidase N and α-2-macroglobulin.
[Mh] Termos MeSH primário: Anthocidaris/citologia
Anthocidaris/imunologia
Proteômica
[Mh] Termos MeSH secundário: Animais
Anthocidaris/genética
Imunidade Celular
Espectrometria de Massas
Proteínas/análise
Proteínas/genética
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Proteins)
[Em] Mês de entrada:1208
[Cu] Atualização por classe:120612
[Lr] Data última revisão:
120612
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120327
[St] Status:MEDLINE
[do] DOI:10.1016/j.dci.2012.03.006



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