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Pesquisa : B01.050.500.408.578.070 [Categoria DeCS]
Referências encontradas : 32 [refinar]
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[PMID]:28188896
[Au] Autor:Cappello T; Vitale V; Oliva S; Villari V; Mauceri A; Fasulo S; Maisano M
[Ad] Endereço:Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
[Ti] Título:Alteration of neurotransmission and skeletogenesis in sea urchin Arbacia lixula embryos exposed to copper oxide nanoparticles.
[So] Source:Comp Biochem Physiol C Toxicol Pharmacol;199:20-27, 2017 Sep.
[Is] ISSN:1532-0456
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The extensive use of copper oxide nanoparticles (CuO NPs) in many applications has raised concerns over their toxicity on environment and human health. Herein, the embryotoxicity of CuO NPs was assessed in the black sea urchin Arbacia lixula, an intertidal species commonly present in the Mediterranean. Fertilized eggs were exposed to 0.7, 10 and 20ppb of CuO NPs, until pluteus stage. Interferences with the normal neurotransmission pathways were observed in sea urchin embryos. In detail, evidence of cholinergic and serotoninergic systems affection was revealed by dose-dependent decreased levels of choline and N-acetyl serotonin, respectively, measured by nuclear magnetic resonance (NMR)-based metabolomics, applied for the first time to our knowledge on sea urchin embryos. The metabolic profile also highlighted a significant CuO NP dose-dependent increase of glycine, a component of matrix proteins involved in the biomineralization process, suggesting perturbed skeletogenesis accordingly to skeletal defects in spicule patterning observed previously in the same sea urchin embryos. However, the expression of skeletogenic genes, i.e. SM30 and msp130, did not differ among groups, and therefore altered primary mesenchyme cell (PMC) migration was hypothesized. Other unknown metabolites were detected from the NMR spectra, and their concentrations found to be reflective of the CuO NP exposure levels. Overall, these findings demonstrate the toxic potential of CuO NPs to interfere with neurotransmission and skeletogenesis of sea urchin embryos. The integrated use of embryotoxicity tests and metabolomics represents a highly sensitive and effective tool for assessing the impact of NPs on aquatic biota.
[Mh] Termos MeSH primário: Arbacia/efeitos dos fármacos
Cobre/toxicidade
Nanopartículas Metálicas/toxicidade
Morfogênese/efeitos dos fármacos
Transmissão Sináptica/efeitos dos fármacos
Poluentes Químicos da Água/toxicidade
Zigoto/efeitos dos fármacos
[Mh] Termos MeSH secundário: Exoesqueleto/efeitos dos fármacos
Exoesqueleto/crescimento & desenvolvimento
Animais
Arbacia/citologia
Arbacia/crescimento & desenvolvimento
Arbacia/fisiologia
Colina/antagonistas & inibidores
Colina/metabolismo
Neurônios Colinérgicos/citologia
Neurônios Colinérgicos/efeitos dos fármacos
Neurônios Colinérgicos/metabolismo
Cobre/química
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Glicina/agonistas
Glicina/metabolismo
Espectroscopia de Ressonância Magnética
Mar Mediterrâneo
Metabolômica/métodos
Nanopartículas Metálicas/química
Neurônios Serotoninérgicos/citologia
Neurônios Serotoninérgicos/efeitos dos fármacos
Serotonina/análogos & derivados
Serotonina/química
Serotonina/metabolismo
Sicília
Propriedades de Superfície
Teratogênios/toxicidade
Poluentes Químicos da Água/química
Zigoto/citologia
Zigoto/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Teratogens); 0 (Water Pollutants, Chemical); 333DO1RDJY (Serotonin); 789U1901C5 (Copper); N91BDP6H0X (Choline); P4TO3C82WV (N-acetylserotonin); TE7660XO1C (Glycine); V1XJQ704R4 (cupric oxide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170212
[St] Status:MEDLINE


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[PMID]:28107742
[Au] Autor:Oral R; Pagano G; Siciliano A; Gravina M; Palumbo A; Castellano I; Migliaccio O; Thomas PJ; Guida M; Tommasi F; Trifuoggi M
[Ad] Endereço:Ege University, Faculty of Fisheries, TR-35100 Bornova, Izmir, Turkey.
[Ti] Título:Heavy rare earth elements affect early life stages in Paracentrotus lividus and Arbacia lixula sea urchins.
[So] Source:Environ Res;154:240-246, 2017 04.
[Is] ISSN:1096-0953
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Heavy rare earth elements (HREEs) have been scarcely studied for their toxicity, in spite of their applications in several technologies. Thus HREEs require timely investigations for their adverse health effects. METHODS: Paracentrotus lividus and Arbacia lixula embryos and sperm were exposed to trichloride salts of five HREEs (Dy, Ho, Er, Yb and Lu) and to Ce(III) as a light REE (LREE) reference to evaluate: 1) developmental defects (% DD) in HREE-exposed larvae or in the offspring of HREE-exposed sperm; 2) mitotic anomalies; 3) fertilization success; and 4) reactive oxygen species (ROS) formation, and nitric oxide (NO) and malondialdehyde (MDA) levels. Nominal HREE concentrations were confirmed by inductively coupled plasma mass spectrometry (ICP-MS). RESULTS: HREEs induced concentration-related DD increases in P. lividus and A. lixula larvae, ranging from no significant DD increase at 10 M HREEs up to ≅100% DD at 10 M HREE. Larvae exposed to 10 M Ce(III) resulted in less severe DD rates compared to HREEs. Decreased mitotic activity and increased aberration rates were found in HREE-exposed P. lividus embryos. Significant increases in ROS formation and NO levels were found both in HREE-exposed and in Ce(III) embryos, whereas only Ce(III), but not HREEs resulted in significant increase in MDA levels. Sperm exposure to HREEs (10 -10 M) resulted in a concentration-related decrease in fertilization success along with increase in offspring damage. These effects were significantly enhanced for Dy(III), Ho(III), Er(III) and Yb(III), compared to Lu(III) and to Ce(III). CONCLUSION: HREE-associated toxicity affected embryogenesis, fertilization, cytogenetic and redox endpoints showing different toxicities of tested HREEs.
[Mh] Termos MeSH primário: Arbacia/efeitos dos fármacos
Desenvolvimento Embrionário/efeitos dos fármacos
Fertilização/efeitos dos fármacos
Larva/efeitos dos fármacos
Metais Terras Raras/toxicidade
Paracentrotus/efeitos dos fármacos
Espermatozoides/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Itália
Masculino
Turquia
Poluentes Químicos da Água/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals, Rare Earth); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE


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[PMID]:27296320
[Au] Autor:Martino C; Bonaventura R; Byrne M; Roccheri M; Matranga V
[Ad] Endereço:Consiglio Nazionale delle Ricerche, Istituto di Biomedicina e Immunologia Molecolare "Alberto Monroy", Via Ugo La Malfa 153, 90146 Palermo, Italy; Dipartimento Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Università di Palermo, Viale delle Scienze, Ed. 16, 90128, Palermo, Italy. Electro
[Ti] Título:Effects of exposure to gadolinium on the development of geographically and phylogenetically distant sea urchins species.
[So] Source:Mar Environ Res;128:98-106, 2017 Jul.
[Is] ISSN:1879-0291
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Gadolinium (Gd), a metal of the lanthanide series used as contrast agent for magnetic resonance imaging, is released into the aquatic environment. We investigated the effects of Gd on the development of four sea urchin species: two from Europe, Paracentrotus lividus and Arbacia lixula, and two from Australia, Heliocidaris tuberculata and Centrostephanus rodgersii. Exposure to Gd from fertilization resulted in inhibition or alteration of skeleton growth in the plutei. The similar morphological response to Gd in the four species indicates a similar mechanism underlying abnormal skeletogenesis. Sensitivity to Gd greatly varied, with the EC50 ranging from 56 nM to 132 µM across the four species. These different sensitivities highlight the importance of testing toxicity in several species for risk assessment. The strong negative effects of Gd on calcification in plutei, together with the plethora of marine species that have calcifying larvae, indicates that Gd pollution is urgent issue that needs to be addressed.
[Mh] Termos MeSH primário: Gadolínio/toxicidade
Paracentrotus/fisiologia
Filogeografia
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Arbacia
Monitoramento Ambiental
Paracentrotus/efeitos dos fármacos
Ouriços-do-Mar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); AU0V1LM3JT (Gadolinium)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160615
[St] Status:MEDLINE


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[PMID]:28080914
[Au] Autor:Applegate JR; Dombrowski DS; Christian LS; Bayer MP; Harms CA; Lewbart GA
[Ti] Título:TRICAINE METHANESULFONATE (MS-222) SEDATION AND ANESTHESIA IN THE PURPLE-SPINED SEA URCHIN (ARBACIA PUNCTULATA).
[So] Source:J Zoo Wildl Med;47(4):1025-1033, 2016 Dec.
[Is] ISSN:1042-7260
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The purple-spined sea urchin ( Arbacia punctulata ) is commonly found in shallow waters of the western Atlantic Ocean from the New England area of the United States to the Caribbean. Sea urchins play a major role in ocean ecology, echinoculture, and biomedical research. Additionally, sea urchins are commonly displayed in public aquaria. Baseline parameters were developed in unanesthetized urchins for righting reflex (time to regain oral recumbency) and spine response time to tactile stimulus. Tricaine methanesulfonate (MS-222) was used to sedate and anesthetize purple-spined sea urchins and assess sedation and anesthetic parameters, including adhesion to and release from a vertical surface, times to loss of response to tactile stimulus and recovery of righting reflex, and qualitative observations of induction of spawning and position of spines and pseudopodia. Sedation and anesthetic parameters were evaluated in 11 individuals in three circumstances: unaltered aquarium water for baseline behaviors, 0.4 g/L MS-222, and 0.8 g/L MS-222. Induction was defined as the release from a vertical surface with the loss of righting reflex, sedation as loss of righting reflex with retained tactile spine response, anesthesia as loss of righting reflex and loss of tactile spine response, and recovery as voluntary return to oral recumbency. MS-222 proved to be an effective sedative and anesthetic for the purple-spined sea urchin at 0.4 and 0.8 g/L, respectively. Sodium bicarbonate used to buffer MS-222 had no measurable sedative effects when used alone. Anesthesia was quickly reversed with transfer of each individual to anesthesia-free seawater, and no anesthetic-related mortality occurred. The parameters assessed in this study provide a baseline for sea urchin anesthesia and may provide helpful comparisons to similar species and populations that are in need of anesthesia for surgical procedures or research.
[Mh] Termos MeSH primário: Aminobenzoatos/farmacologia
Anestesia
Anestésicos/farmacologia
Arbacia/efeitos dos fármacos
[Mh] Termos MeSH secundário: Aminobenzoatos/administração & dosagem
Anestésicos/administração & dosagem
Animais
Relação Dose-Resposta a Droga
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aminobenzoates); 0 (Anesthetics); 02591PHL19 (tricaine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.1638/2015-0288.1


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[PMID]:27421237
[Au] Autor:Castellano GC; Souza MM; Freire CA
[Ad] Endereço:Programa de Pós-Graduação em Zoologia, UFPR, Curitiba, PR, Brazil. Electronic address: gccastellano@hotmail.com.
[Ti] Título:Volume regulation of intestinal cells of echinoderms: Putative role of ion transporters (Na(+)/K(+)-ATPase and NKCC).
[So] Source:Comp Biochem Physiol A Mol Integr Physiol;201:124-131, 2016 Nov.
[Is] ISSN:1531-4332
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Echinoderms are exclusively marine osmoconformer invertebrates. Some species occupy the challenging intertidal region. Upon salinity changes, the extracellular osmotic concentration of these animals also varies, exposing tissues and cells to osmotic challenges. Cells and tissues may then respond with volume regulation mechanisms, which involve transport of ions and water into and/or out of the cells, through ion transporters, such as the Na(+)/K(+)-ATPase and NKCC. The goal of this study was to relate the cell volume regulation capacity of echinoderm intestinal cells Na(+)/K(+)-ATPase and NKCC activities, in three echinoderm species: Holothuria grisea, Arbacia lixula, and Echinometra lucunter. Isolated cells of these species displayed some control of their cell volume upon exposure to anisosmotic media (isolated intestinal cells, calcein fluorescence as indicator of volume change), with a distinct higher capacity shown by H. grisea, which did not swell even upon 50% hyposmotic shock. The holothuroid cells showed indirect evidence (effect of furosemide) of the participation of NKCC in this process, with a secretory function, and of a secondary role by the NKA (effect of ouabain). Other mechanisms are probably responsible for this function in the urchins. Variable expression of these transporters, and others not examined here, may to some extent account for the variability in cell volume regulation capacity in echinoderm cells.
[Mh] Termos MeSH primário: Equinodermos/citologia
Equinodermos/metabolismo
Simportadores de Cloreto de Sódio-Potássio/metabolismo
ATPase Trocadora de Sódio-Potássio/metabolismo
[Mh] Termos MeSH secundário: Animais
Arbacia/citologia
Arbacia/metabolismo
Tamanho Celular
Holothuria/citologia
Holothuria/metabolismo
Intestinos/citologia
Intestinos/metabolismo
Pressão Osmótica
Ouriços-do-Mar/citologia
Ouriços-do-Mar/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sodium-Potassium-Chloride Symporters); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171008
[Lr] Data última revisão:
171008
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160717
[St] Status:MEDLINE


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[PMID]:27208032
[Au] Autor:Wheeler JD; Chan KY; Anderson EJ; Mullineaux LS
[Ad] Endereço:Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA.
[Ti] Título:Ontogenetic changes in larval swimming and orientation of pre-competent sea urchin Arbacia punctulata in turbulence.
[So] Source:J Exp Biol;219(Pt 9):1303-10, 2016 May 01.
[Is] ISSN:1477-9145
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many marine organisms have complex life histories, having sessile adults and relying on the planktonic larvae for dispersal. Larvae swim and disperse in a complex fluid environment and the effect of ambient flow on larval behavior could in turn impact their survival and transport. However, to date, most studies on larvae-flow interactions have focused on competent larvae near settlement. We examined the importance of flow on early larval stages by studying how local flow and ontogeny influence swimming behavior in pre-competent larval sea urchins, Arbacia punctulata We exposed larval urchins to grid-stirred turbulence and recorded their behavior at two stages (4- and 6-armed plutei) in three turbulence regimes. Using particle image velocimetry to quantify and subtract local flow, we tested the hypothesis that larvae respond to turbulence by increasing swimming speed, and that the increase varies with ontogeny. Swimming speed increased with turbulence for both 4- and 6-armed larvae, but their responses differed in terms of vertical swimming velocity. 4-Armed larvae swam most strongly upward in the unforced flow regime, while 6-armed larvae swam most strongly upward in weakly forced flow. Increased turbulence intensity also decreased the relative time that larvae spent in their typical upright orientation. 6-Armed larvae were tilted more frequently in turbulence compared with 4-armed larvae. This observation suggests that as larvae increase in size and add pairs of arms, they are more likely to be passively re-oriented by moving water, rather than being stabilized (by mechanisms associated with increased mass), potentially leading to differential transport. The positive relationship between swimming speed and larval orientation angle suggests that there was also an active response to tilting in turbulence. Our results highlight the importance of turbulence to planktonic larvae, not just during settlement but also in earlier stages through morphology-flow interactions.
[Mh] Termos MeSH primário: Arbacia/fisiologia
[Mh] Termos MeSH secundário: Animais
Arbacia/anatomia & histologia
Arbacia/crescimento & desenvolvimento
Tamanho Corporal
Hidrodinâmica
Larva/anatomia & histologia
Larva/crescimento & desenvolvimento
Larva/fisiologia
Orientação Espacial
Reologia
Natação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160522
[St] Status:MEDLINE
[do] DOI:10.1242/jeb.129502


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[PMID]:25920999
[Au] Autor:Nikishin DA; Milosevic I; Gojkovic M; Rakic L; Bezuglov VV; Shmukler YB
[Ad] Endereço:N.K. Koltzov Institute of Developmental Biology,Russian Academy of Sciences,Moscow,Russia.
[Ti] Título:Expression and functional activity of neurotransmitter system components in sea urchins' early development.
[So] Source:Zygote;24(2):206-18, 2016 Apr.
[Is] ISSN:1469-8730
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Reverse-transcription polymerase chain reaction (RT-PCR) investigation of the expression of the components supposedly taking part in serotonin regulation of the early development of Paracentrotus lividus has shown the presence of transcripts of five receptors, one of which has conservative amino acid residues characteristic of monoaminergic receptors. At the early stages of embryogenesis the expressions of serotonin transporter (SERT) and noradrenaline transporter (NET) were also recognized. The activities of the enzymes of serotonin synthesis and serotonin transporter were shown using immunohistochemistry and incubation with para-chlorophenylalanine (PСРА) and 5-hydroxytryptophan (HTP). Pharmacological experiments have shown a preferential cytostatic activity of ligands characterized as mammalian 5-hydroxytryptamine (5-HT)1-antagonists. On the basis of the sum of the data from molecular biology and embryo physiological experiments, it is suggested that metabotropic serotonin receptors and membrane transporters take part in the regulatory processes of early sea urchin embryogenesis.
[Mh] Termos MeSH primário: Arbacia/genética
Embrião não Mamífero/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Proteínas de Transporte de Neurotransmissores/genética
Paracentrotus/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Arbacia/embriologia
Arbacia/metabolismo
Proteínas da Membrana Plasmática de Transporte de Dopamina/genética
Proteínas da Membrana Plasmática de Transporte de Dopamina/metabolismo
Embrião não Mamífero/embriologia
Imuno-Histoquímica
Proteínas de Transporte de Neurotransmissores/metabolismo
Proteínas da Membrana Plasmática de Transporte de Norepinefrina/genética
Proteínas da Membrana Plasmática de Transporte de Norepinefrina/metabolismo
Paracentrotus/embriologia
Paracentrotus/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Homologia de Sequência de Aminoácidos
Proteínas da Membrana Plasmática de Transporte de Serotonina/genética
Proteínas da Membrana Plasmática de Transporte de Serotonina/metabolismo
Fatores de Tempo
Proteínas Vesiculares de Transporte de Monoamina/genética
Proteínas Vesiculares de Transporte de Monoamina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dopamine Plasma Membrane Transport Proteins); 0 (Neurotransmitter Transport Proteins); 0 (Norepinephrine Plasma Membrane Transport Proteins); 0 (Serotonin Plasma Membrane Transport Proteins); 0 (Vesicular Monoamine Transport Proteins)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150430
[St] Status:MEDLINE
[do] DOI:10.1017/S0967199415000040


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[PMID]:26278469
[Au] Autor:Jikeli JF; Alvarez L; Friedrich BM; Wilson LG; Pascal R; Colin R; Pichlo M; Rennhack A; Brenker C; Kaupp UB
[Ad] Endereço:Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.
[Ti] Título:Sperm navigation along helical paths in 3D chemoattractant landscapes.
[So] Source:Nat Commun;6:7985, 2015 Aug 17.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sperm require a sense of direction to locate the egg for fertilization. They follow gradients of chemical and physical cues provided by the egg or the oviduct. However, the principles underlying three-dimensional (3D) navigation in chemical landscapes are unknown. Here using holographic microscopy and optochemical techniques, we track sea urchin sperm navigating in 3D chemoattractant gradients. Sperm sense gradients on two timescales, which produces two different steering responses. A periodic component, resulting from the helical swimming, gradually aligns the helix towards the gradient. When incremental path corrections fail and sperm get off course, a sharp turning manoeuvre puts sperm back on track. Turning results from an 'off' Ca(2+) response signifying a chemoattractant stimulation decrease and, thereby, a drop in cyclic GMP concentration and membrane voltage. These findings highlight the computational sophistication by which sperm sample gradients for deterministic klinotaxis. We provide a conceptual and technical framework for studying microswimmers in 3D chemical landscapes.
[Mh] Termos MeSH primário: Arbacia/fisiologia
Fatores Quimiotáticos/farmacologia
Quimiotaxia/fisiologia
Espermatozoides/efeitos dos fármacos
Espermatozoides/fisiologia
[Mh] Termos MeSH secundário: Animais
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemotactic Factors)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150818
[St] Status:MEDLINE
[do] DOI:10.1038/ncomms8985


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[PMID]:26164225
[Au] Autor:Buric P; Jaksic Z; Stajner L; Dutour Sikiric M; Jurasin D; Cascio C; Calzolai L; Lyons DM
[Ad] Endereço:Center for Marine Research, Ruder Boskovic Institute, Giordano Paliaga 5, 52210 Rovinj, Croatia.
[Ti] Título:Effect of silver nanoparticles on Mediterranean sea urchin embryonal development is species specific and depends on moment of first exposure.
[So] Source:Mar Environ Res;111:50-9, 2015 Oct.
[Is] ISSN:1879-0291
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:With the ever growing use of nanoparticles in a broad range of industrial and consumer applications there is increasing likelihood that such nanoparticles will enter the aquatic environment and be transported through freshwater systems, eventually reaching estuarine or marine waters. Due to silver's known antimicrobial properties and widespread use of silver nanoparticles (AgNP), their environmental fate and impact is therefore of particular concern. In this context we have investigated the species-specific effects of low concentrations of 60 nm AgNP on embryonal development in Mediterranean sea urchins Arbacia lixula, Paracentrotus lividus and Sphaerechinus granularis. The sensitivity of urchin embryos was tested by exposing embryos to nanoparticle concentrations in the 1-100 µg L(-1) range, with times of exposure varying from 30 min to 24 h (1 h-48 h for S. granularis) post-fertilisation which corresponded with fertilized egg, 4 cell, blastula and gastrula development phases. The most sensitive species to AgNP was A. lixula with significant modulation of embryonal development at the lowest AgNP concentrations of 1-10 µg L(-1) with high numbers of malformed embryos or arrested development. The greatest impact on development was noted for those embryos first exposed to nanoparticles at 6 and 24 h post fertilisation. For P. lividus, similar effects were noted at higher concentrations of 50 µg L(-1) and 100 µg L(-1) for all times of first exposure. The S. granularis embryos indicated a moderate AgNP impact, and significant developmental abnormalities were recorded in the concentration range of 10-50 µg L(-1). As later post-fertilisation exposure times to AgNP caused greater developmental changes in spite of a shorter total exposure time led us to postulate on additional mechanisms of AgNP toxicity. The results herein indicate that toxic effects of AgNP are species-specific. The moment at which embryos first encounter AgNP is also shown to be an important factor in the development of abnormalities, and future applications of the sea urchin embryo development test for nanoparticle toxicity testing should carefully address the specific phase of development of embryos when nanoparticles are first introduced.
[Mh] Termos MeSH primário: Desenvolvimento Embrionário/efeitos dos fármacos
Exposição Ambiental
Nanopartículas Metálicas/toxicidade
Ouriços-do-Mar/efeitos dos fármacos
Prata/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Arbacia/efeitos dos fármacos
Arbacia/embriologia
Croácia
Relação Dose-Resposta a Droga
Mar Mediterrâneo
Paracentrotus/efeitos dos fármacos
Paracentrotus/embriologia
Ouriços-do-Mar/embriologia
Especificidade da Espécie
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); 3M4G523W1G (Silver)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151030
[Lr] Data última revisão:
151030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150713
[St] Status:MEDLINE


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[PMID]:26026240
[Au] Autor:Maisano M; Cappello T; Catanese E; Vitale V; Natalotto A; Giannetto A; Barreca D; Brunelli E; Mauceri A; Fasulo S
[Ad] Endereço:Department of Biological and Environmental Sciences, University of Messina, Messina, Italy. Electronic address: mmaisano@unime.it.
[Ti] Título:Developmental abnormalities and neurotoxicological effects of CuO NPs on the black sea urchin Arbacia lixula by embryotoxicity assay.
[So] Source:Mar Environ Res;111:121-7, 2015 Oct.
[Is] ISSN:1879-0291
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The embryotoxicity of CuO NPs was evaluated in the black sea urchin Arbacia lixula embryos, by using 24-well plates. Fertilized eggs were exposed to five doses of CuO NPs ranging from 0.07 to 20 ppb, until pluteus stage. CuO NPs suspensions in artificial seawater formed agglomerates of 80-200 nm size, and copper uptake was 2.5-fold up in larvae exposed to high NP concentrations in respect to control. Developmental delay and morphological alteration, including skeletal abnormalities, were observed, as well as impairment in cholinergic and serotonergic nervous systems. These findings suggest the potential of CuO NPs to interfere with the normal neurotransmission pathways, thus affecting larval morphogenesis. Overall, the embryotoxicity tests are effective for evaluation of nanoparticle effects on the health of aquatic biota. Furthermore, as the black sea urchin A. lixula demonstrated to be vulnerable to NP exposure, it may be a valid bioindicator in marine biomonitoring and ecotoxicological programmes.
[Mh] Termos MeSH primário: Arbacia/efeitos dos fármacos
Cobre/toxicidade
Desenvolvimento Embrionário/efeitos dos fármacos
Nanopartículas Metálicas/toxicidade
Neurotoxinas/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Arbacia/embriologia
Arbacia/crescimento & desenvolvimento
Larva/efeitos dos fármacos
Larva/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neurotoxins); 0 (Water Pollutants, Chemical); 789U1901C5 (Copper); V1XJQ704R4 (cupric oxide)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151030
[Lr] Data última revisão:
151030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150601
[St] Status:MEDLINE



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