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[PMID]:28837417
[Au] Autor:Sahlmann A; Wolf R; Holth TF; Titelman J; Hylland K
[Ad] Endereço:a Department of Biosciences , University of Oslo , Oslo , Norway.
[Ti] Título:Baseline and oxidative DNA damage in marine invertebrates.
[So] Source:J Toxicol Environ Health A;80(16-18):807-819, 2017.
[Is] ISSN:1528-7394
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Anthropogenic pollutants produce oxidative stress in marine organisms, directly or following generation of reactive oxygen species (ROS), potentially resulting in increased accumulation of DNA strand breaks quantified. The aim of this study is to quantify baseline levels of DNA strand breaks in marine species from four phyla and to assess relative sensitivity to oxidative stress as well as ability to recover. DNA strand breaks were determined using a formamidopyrimidine DNA glycosylase (Fpg)-amended comet assay in circulating cells from blue mussel (Mytilus edulis), shore crab (Carcinus maenas), sea star (Asterias rubens), and vase tunicate (Ciona intestinalis). Lymphocytes from Atlantic cod (Gadus morhua) were used as a reference. In addition to immediate analysis, cells from all species were exposed ex vivo to two concentrations of hydrogen peroxide (H O ) at 25 or 250 µM prior to assay. Mean baseline DNA strand breaks were highest for cells from sea star (34%) followed by crab (25%), mussel (22%), tunicate (17%), and cod (14%). Circulating cells from invertebrates were markedly more sensitive to oxidative stress compared to cod lymphocytes. DNA strand breaks exceeded 80% for sea star, crab, and mussel cells following exposure to the lowest H O concentration. There was no recovery for cells from any species following 1 hr in buffer. This study provides an in-depth analysis of DNA integrity for ecologically important species representing 4 phyla. Data indicate that circulating cells from invertebrates are more sensitive to oxidative stress than cells from fish as evidenced by DNA strand breaks. Future studies need to address the extent to which DNA strand breaks may exert consequences for body maintenance costs in marine invertebrates.
[Mh] Termos MeSH primário: Organismos Aquáticos/efeitos dos fármacos
Dano ao DNA/efeitos dos fármacos
Peróxido de Hidrogênio/toxicidade
Estresse Oxidativo/efeitos dos fármacos
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Organismos Aquáticos/genética
Asterias/efeitos dos fármacos
Asterias/genética
Braquiúros/efeitos dos fármacos
Braquiúros/genética
Ciona intestinalis/efeitos dos fármacos
Ciona intestinalis/genética
Ensaio Cometa
DNA-Formamidopirimidina Glicosilase/metabolismo
Determinação de Ponto Final
Peixes/genética
Linfócitos/efeitos dos fármacos
Linfócitos/metabolismo
Mytilus edulis/efeitos dos fármacos
Mytilus edulis/genética
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); BBX060AN9V (Hydrogen Peroxide); EC 3.2.2.23 (DNA-Formamidopyrimidine Glycosylase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1080/15287394.2017.1352179


  2 / 90 MEDLINE  
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[PMID]:27682169
[Au] Autor:Petrov NB; Vladychenskaya IP; Drozdov AL; Kedrova OS
[Ad] Endereço:Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology, Moscow, 119991, Russia. petr@belozersky.msu.ru.
[Ti] Título:Molecular Genetic Markers of Intra- and Interspecific Divergence within Starfish and Sea Urchins (Echinodermata).
[So] Source:Biochemistry (Mosc);81(9):972-80, 2016 Sep.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A fragment of the mitochondrial COI gene from isolates of several echinoderm species was sequenced. The isolates were from three species of starfish from the Asteriidae family (Asterias amurensis and Aphelasterias japonica collected in the Sea of Japan and Asterias rubens collected in the White Sea) and from the sea urchin Echinocardium cordatum (family Loveniidae) collected in the Sea of Japan. Additionally, regions including internal transcribed spacers and 5.8S rRNA (ITS1 - 5.8S rDNA - ITS2) were sequenced for the three studied starfish species. Phylogenetic analysis of the obtained COI sequences together with earlier determined homologous COI sequences from Ast. forbesii, Ast. rubens, and Echinocardium laevigaster from the North Atlantic and E. cordatum from the Yellow and North Seas (GenBank) placed them into strictly conspecific clusters with high bootstrap support (99% in all cases). Only two exceptions - Ast. rubens DQ077915 sequence placed with the Ast. forbesii cluster and Aph. japonica DQ992560 sequence placed with the Ast. amurensis cluster - were likely results of species misidentification. The intraspecific polymorphism for the COI gene within the Asteriidae family varied within a range of 0.2-0.9% as estimated from the genetic distances. The corresponding intrageneric and intergeneric values were 10.4-12.1 and 21.8-29.8%, respectively. The interspecific divergence for the COI gene in the sea urchin of Echinocardium genus (family Loveniidae) was significantly higher (17.1-17.7%) than in the starfish, while intergeneric divergence (14.6-25.7%) was similar to that in asteroids. The interspecific genetic distances for the nuclear transcribed sequences (ITS1 - 5.8S rDNA - ITS2) within the Asteriidae family were lower (3.1-4.5%), and the intergeneric distances were significantly higher (32.8-35.0%), compared to the corresponding distances for the COI gene. These results suggest that the investigated molecular-genetic markers could be used for segregation and identification of echinoderm species.
[Mh] Termos MeSH primário: Asterias/genética
Complexo IV da Cadeia de Transporte de Elétrons/genética
Evolução Molecular
Proteínas Mitocondriais/genética
Filogenia
Ouriços-do-Mar/genética
[Mh] Termos MeSH secundário: Animais
Asterias/enzimologia
Marcadores Genéticos
Polimorfismo Genético
Ouriços-do-Mar/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); 0 (Mitochondrial Proteins); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160930
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297916090066


  3 / 90 MEDLINE  
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[PMID]:27552100
[Au] Autor:Richardson MF; Sherman CD; Lee RS; Bott NJ; Hirst AJ
[Ad] Endereço:Bioinformatics Core Research Group, Deakin University, 75 Pigdons Road, Locked Bag 20000, Geelong, Vic., 3220, Australia. m.richardson@deakin.edu.au.
[Ti] Título:Multiple dispersal vectors drive range expansion in an invasive marine species.
[So] Source:Mol Ecol;25(20):5001-5014, 2016 Oct.
[Is] ISSN:1365-294X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The establishment and subsequent spread of invasive species is widely recognized as one of the most threatening processes contributing to global biodiversity loss. This is especially true for marine and estuarine ecosystems, which have experienced significant increases in the number of invasive species with the increase in global maritime trade. Understanding the rate and mechanisms of range expansion is therefore of significant interest to ecologists and conservation managers alike. Using a combination of population genetic surveys, environmental DNA (eDNA) plankton sampling and hydrodynamic modelling, we examined the patterns of introduction of the predatory Northern Pacific seastar (Asterias amurensis) and pathways of secondary spread within southeast Australia. Genetic surveys across the invasive range reveal some genetic divergence between the two main invasive regions and no evidence of ongoing gene flow, a pattern that is consistent with the establishment of the second invasive region via a human-mediated translocation event. In contrast, hydrodynamic modelling combined with eDNA plankton sampling demonstrated that the establishment of range expansion populations within a region is consistent with natural larval dispersal and recruitment. Our results suggest that both anthropogenic and natural dispersal vectors have played an important role in the range expansion of this species in Australia. The multiple modes of spread combined with high levels of fecundity and a long larval duration in A. amurensis suggests it is likely to continue its range expansion and significantly impact Australian marine ecosystems.
[Mh] Termos MeSH primário: Distribuição Animal
Asterias/genética
Genética Populacional
Espécies Introduzidas
[Mh] Termos MeSH secundário: Animais
Austrália
Ecossistema
Variação Genética
Técnicas de Genotipagem
Hidrodinâmica
Larva
Repetições de Microssatélites
Plâncton
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160824
[St] Status:MEDLINE
[do] DOI:10.1111/mec.13817


  4 / 90 MEDLINE  
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[PMID]:27430385
[Au] Autor:Mikami D; Sakai S; Sasaki S; Igarashi Y
[Ad] Endereço:Laboratory of Biomembrane and Biofunctional Chemistry, Graduate School of Advanced Life Science, and Frontier Research Center for Post-Genome Science and Technology, Hokkaido University.
[Ti] Título:Effects of Asterias amurensis-derived Sphingoid Bases on the de novo Ceramide Synthesis in Cultured Normal Human Epidermal Keratinocytes.
[So] Source:J Oleo Sci;65(8):671-80, 2016 Aug 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Asterias amurensis starfish provide several bioactive species in addition to being fishery waste. Glucosyl ceramides (GlcCers) were extracted from the viscera of these starfish and were isolated by silica gel column chromatography. Degraded GlcCers generated A. amurensis sphingoid bases (ASBs) that mainly consisted of the triene-type bases d18:3 and 9-methyl-d18:3. The effect of these bases on ceramide synthesis and content were analyzed using normal human epidermal keratinocytes (NHEKs). The bases significantly enhanced the de novo ceramide synthesis and gene expression in NHEKs for proteins, such as serine-palmitoyltransferase and ceramide synthase. Total ceramide, GlcCer, and sphingomyelin contents increased dramatically upon ASB treatment. In particular, GlcCer bearing very-long-chain fatty acids (≥C28) exhibited a significant content increase. These ASB-induced enhancements on de novo ceramide synthesis were only observed in undifferentiated NHEKs. This stimulation of the de novo sphingolipid synthesis may improve skin barrier functions.
[Mh] Termos MeSH primário: Asterias/química
Ceramidas/biossíntese
Epiderme/citologia
Queratinócitos/efeitos dos fármacos
Queratinócitos/metabolismo
Esfingosina/isolamento & purificação
Esfingosina/farmacologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Ceramidas/química
Relação Dose-Resposta a Droga
Seres Humanos
Estrutura Molecular
Esfingosina/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ceramides); NGZ37HRE42 (Sphingosine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160720
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess16051


  5 / 90 MEDLINE  
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[PMID]:27411825
[Au] Autor:Ezhova OV; Egorova EA; Malakhov VV
[Ad] Endereço:Moscow State University, Moscow, 119992, Russia. olga.ejova@gmail.com.
[Ti] Título:Ultrastructural evidence of the excretory function in the asteroid axial organ (Asteroidea, Echinodermata).
[So] Source:Dokl Biol Sci;468(1):129-32, 2016 May.
[Is] ISSN:1608-3105
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ultrastructure of the axial organ of Asterias amurensis has been studied The organ is a network of canals of the axial coelom separated by haemocoelic spaces. The axial coelom is lined with two types of monociliary cells: podocytes and musculo-epithelial cells. Podocytes form numerous basal processes adjacent to the basal lamina on the coelomic side. Musculo-epithelial cells form processes running along the basal lamina. Some bundles of these processes wrapped in the basal lamina pass through haemocoelic spaces between neighboring coelomic canals. It is hypothesized that the axial organ serves for filtration of fluid from haemocoelic spaces into the axial coelom cavity, from which urine is excreted through the madreporite to the exterior.
[Mh] Termos MeSH primário: Asterias
Células Epiteliais
Células Musculares
Podócitos
[Mh] Termos MeSH secundário: Animais
Asterias/metabolismo
Asterias/ultraestrutura
Células Epiteliais/secreção
Células Epiteliais/ultraestrutura
Células Musculares/secreção
Células Musculares/ultraestrutura
Podócitos/secreção
Podócitos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160715
[St] Status:MEDLINE
[do] DOI:10.1134/S0012496616030091


  6 / 90 MEDLINE  
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[PMID]:27029504
[Au] Autor:Byrne M; Gall M; Wolfe K; Agüera A
[Ad] Endereço:Schools of Medical and Biological Sciences, University of Sydney, Sydney, NSW, 2006, Australia.
[Ti] Título:From pole to pole: the potential for the Arctic seastar Asterias amurensis to invade a warming Southern Ocean.
[So] Source:Glob Chang Biol;22(12):3874-3887, 2016 Dec.
[Is] ISSN:1365-2486
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Due to climatic warming, Asterias amurensis, a keystone boreal predatory seastar that has established extensive invasive populations in southern Australia, is a potential high-risk invader of the sub-Antarctic and Antarctic. To assess the potential range expansion of A. amurensis to the Southern Ocean as it warms, we investigated the bioclimatic envelope of the adult and larval life stages. We analysed the distribution of adult A. amurensis with respect to present-day and future climate scenarios using habitat temperature data to construct species distribution models (SDMs). To integrate the physiological response of the dispersive phase, we determined the thermal envelope of larval development to assess their performance in present-day and future thermal regimes and the potential for success of A. amurensis in poleward latitudes. The SDM indicated that the thermal 'niche' of the adult stage correlates with a 0-17 °C and 1-22.5 °C range, in winter and summer, respectively. As the ocean warms, the range of A. amurensis in Australia will contract, while more southern latitudes will have conditions favourable for range expansion. Successful fertilization occurred from 3 to 23.8 °C. By day 12, development to the early larval stage was successful from 5.5 to 18 °C. Although embryos were able to reach the blastula stage at 2 °C, they had arrested development and high mortality. The optimal thermal range for survival of pelagic stages was 3.5-19.2 °C with a lower and upper critical limit of 2.6 and 20.3 °C, respectively. Our data predict that A. amurensis faces demise in its current invasive range while more favourable conditions at higher latitudes would facilitate invasion of both larval and adult stages to the Southern Ocean. Our results show that vigilance is needed to reduce the risk that this ecologically important Arctic carnivore may invade the Southern Ocean and Antarctica.
[Mh] Termos MeSH primário: Distribuição Animal
Asterias
Mudança Climática
[Mh] Termos MeSH secundário: Animais
Regiões Antárticas
Regiões Árticas
Austrália
Espécies Introduzidas
Modelos Teóricos
Oceanos e Mares
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160401
[St] Status:MEDLINE
[do] DOI:10.1111/gcb.13304


  7 / 90 MEDLINE  
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[PMID]:27001385
[Au] Autor:Du L; Yang YH; Xu J; Wang YM; Xue CH; Kurihara H; Takahashi K
[Ad] Endereço:Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido 041-8611, Japan. kore@fish.hokudai.ac.jp.
[Ti] Título:Transport and uptake effects of marine complex lipid liposomes in small intestinal epithelial cell models.
[So] Source:Food Funct;7(4):1904-14, 2016 Apr.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nowadays, marine complex lipids, including starfish phospholipids (SFP) and cerebrosides (SFC) separated from Asterias amurensis as well as sea cucumber phospholipids (SCP) and cerebrosides (SCC) isolated from Cucumaria frondosa, have received much attention because of their potent biological activities. However, little information is known on the transport and uptake of these lipids in liposome forms in small intestinal cells. Therefore, this study was undertaken to investigate the effects of these complex lipid liposomes on transport and uptake in Caco-2 and M cell monolayer models. The results revealed that SFP and SCP contained 42% and 47.9% eicosapentaenoic acid (EPA), respectively. The average particle sizes of liposomes prepared in this study were from 169 to 189 nm. We found that the transport of the liposomes across the M cell monolayer model was much higher than the Caco-2 cell monolayer model. The liposomes consisting of SFP or SCP showed significantly higher transport and uptake than soy phospholipid (soy-PL) liposomes in both Caco-2 and M cell monolayer models. Our results also exhibited that treatment with 1 mM liposomes composed of SFP or SCP for 3 h tended to increase the EPA content in phospholipid fractions of both differentiated Caco-2 and M cells. Moreover, it was also found that the hybrid liposomes consisting of SFP/SFC/cholesterol (Chol) revealed higher transport and uptake across the M cell monolayer in comparison with other liposomes. Furthermore, treatment with SFP/SFC/Chol liposomes could notably decrease the trans-epithelial electrical resistance (TEER) values of Caco-2 and M cell monolayers. The present data also showed that the cell viability of differentiated Caco-2 and M cells was not affected after the treatment with marine complex lipids or soy-PL liposomes. Based on the data in this study, it was suggested that marine complex lipid liposomes exhibit prominent transport and uptake in small intestinal epithelial cell models.
[Mh] Termos MeSH primário: Asterias/química
Cerebrosídeos/metabolismo
Cucumaria/química
Células Epiteliais/metabolismo
Intestino Delgado/metabolismo
Lipossomos/metabolismo
Fosfolipídeos/metabolismo
Alimentos Marinhos/análise
[Mh] Termos MeSH secundário: Animais
Transporte Biológico
Células CACO-2
Sobrevivência Celular
Cerebrosídeos/química
Seres Humanos
Lipossomos/química
Modelos Biológicos
Fosfolipídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cerebrosides); 0 (Liposomes); 0 (Phospholipids)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170110
[Lr] Data última revisão:
170110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160323
[St] Status:MEDLINE
[do] DOI:10.1039/c6fo00066e


  8 / 90 MEDLINE  
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[PMID]:26920046
[Au] Autor:Motoyoshi N; Kobayashi H; Itagaki T; Inokuchi N
[Ad] Endereço:School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funahashi, Chiba 274-8555, Japan.
[Ti] Título:Amino acid sequence analysis and characterization of a ribonuclease from starfish Asterias amurensis.
[So] Source:J Biochem;160(3):131-9, 2016 Sep.
[Is] ISSN:1756-2651
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to phylogenetically characterize the location of the RNase T2 enzyme in the starfish (Asterias amurensis). We isolated an RNase T2 ribonuclease (RNase Aa) from the ovaries of starfish and determined its amino acid sequence by protein chemistry and cloning cDNA encoding RNase Aa. The isolated protein had 231 amino acid residues, a predicted molecular mass of 25,906 Da, and an optimal pH of 5.0. RNase Aa preferentially released guanylic acid from the RNA. The catalytic sites of the RNase T2 family are conserved in RNase Aa; furthermore, the distribution of the cysteine residues in RNase Aa is similar to that in other animal and plant T2 RNases. RNase Aa is cleaved at two points: 21 residues from the N-terminus and 29 residues from the C-terminus; however, both fragments may remain attached to the protein via disulfide bridges, leading to the maintenance of its conformation, as suggested by circular dichroism spectrum analysis. The phylogenetic analysis revealed that starfish RNase Aa is evolutionarily an intermediate between protozoan and oyster RNases.
[Mh] Termos MeSH primário: Asterias
Endorribonucleases
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Asterias/enzimologia
Asterias/genética
Endorribonucleases/química
Endorribonucleases/genética
Ribonuclease Pancreático/química
Ribonuclease Pancreático/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.1.- (Endoribonucleases); EC 3.1.27.1 (ribonuclease T(2)); EC 3.1.27.5 (Ribonuclease, Pancreatic)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160228
[St] Status:MEDLINE
[do] DOI:10.1093/jb/mvw017


  9 / 90 MEDLINE  
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[PMID]:26801824
[Au] Autor:Kim CH; Kim EJ; Go HJ; Oh HY; Lin M; Elphick MR; Park NG
[Ad] Endereço:Department of Biotechnology, College of Fisheries Sciences, Pukyong National University, Busan, Korea.
[Ti] Título:Identification of a novel starfish neuropeptide that acts as a muscle relaxant.
[So] Source:J Neurochem;137(1):33-45, 2016 Apr.
[Is] ISSN:1471-4159
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Neuropeptides that act as muscle relaxants have been identified in chordates and protostomian invertebrates but little is known about the molecular identity of neuropeptides that act as muscle relaxants in deuterostomian invertebrates (e.g. echinoderms) that are 'evolutionary intermediates' of chordates and protostomes. Here, we have used the apical muscle of the starfish Patiria pectinifera to assay for myorelaxants in extracts of this species. A hexadecapeptide with the amino acid sequence Phe-Gly-Lys-Gly-Gly-Ala-Tyr-Asp-Pro-Leu-Ser-Ala-Gly-Phe-Thr-Asp was identified and designated starfish myorelaxant peptide (SMP). Cloning and sequencing of a cDNA encoding the SMP precursor protein revealed that it comprises 12 copies of SMP as well as 3 peptides (7 copies in total) that are structurally related to SMP. Analysis of the expression of SMP precursor transcripts in P. pectinifera using qPCR revealed the highest expression in the radial nerve cords and lower expression levels in a range of neuromuscular tissues, including the apical muscle, tube feet and cardiac stomach. Consistent with these findings, SMP also caused relaxation of tube foot and cardiac stomach preparations. Furthermore, SMP caused relaxation of apical muscle preparations from another starfish species - Asterias amurensis. Collectively, these data indicate that SMP has a general physiological role as a muscle relaxant in starfish. Interestingly, comparison of the sequence of the SMP precursor with known neuropeptide precursors revealed that SMP belongs to a bilaterian family of neuropeptides that include molluscan pedal peptides (PP) and arthropodan orcokinins (OK). This is the first study to determine the function of a PP/OK-type peptide in a deuterostome. Pedal peptide/orcokinin (PP/OK)-type peptides are a family of structurally related neuropeptides that were first identified and functionally characterised in protostomian invertebrates. Here, we report the discovery of starfish myorelaxant peptide (SMP), a novel member of the PP/OK-type neuropeptide identified in the starfish Patiria pectinifera (phylum Echinodermata). SMP is the first PP/OK-type neuropeptide to be functionally characterised in a deuterostome.
[Mh] Termos MeSH primário: Relaxamento Muscular/efeitos dos fármacos
Fármacos Neuromusculares/isolamento & purificação
Neuropeptídeos/isolamento & purificação
Estrelas-do-Mar/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Asterias
Sequência de Bases
Clonagem Molecular
DNA Complementar/genética
Dados de Sequência Molecular
Músculos/efeitos dos fármacos
Neuropeptídeos/genética
Neuropeptídeos/farmacologia
Neuropeptídeos/fisiologia
Precursores de Proteínas/genética
RNA Mensageiro/biossíntese
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Neuromuscular Agents); 0 (Neuropeptides); 0 (Protein Precursors); 0 (RNA, Messenger); 0 (starfish myorelaxant peptide, Patiria pectinifera)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160124
[St] Status:MEDLINE
[do] DOI:10.1111/jnc.13543


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[PMID]:26529321
[Au] Autor:Richardson MF; Sherman CD
[Ad] Endereço:Deakin University, Geelong, Australia. School of Life and Environmental Sciences, Centre for Integrative Ecology, (Waurn Ponds Campus). 75 Pigdons Road. Locked Bag 20000, Geelong, VIC 3220, Australia.
[Ti] Título:De Novo Assembly and Characterization of the Invasive Northern Pacific Seastar Transcriptome.
[So] Source:PLoS One;10(11):e0142003, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Invasive species are a major threat to global biodiversity but can also serve as valuable model systems to examine important evolutionary processes. While the ecological aspects of invasions have been well documented, the genetic basis of adaptive change during the invasion process has been hampered by a lack of genomic resources for the majority of invasive species. Here we report the first larval transcriptomic resource for the Northern Pacific Seastar, Asterias amurensis, an invasive marine predator in Australia. Approximately 117.5 million 100 base-pair (bp) paired-end reads were sequenced from a single RNA-Seq library from a pooled set of full-sibling A. amurensis bipinnaria larvae. We evaluated the efficacy of a pre-assembly error correction pipeline on subsequent de novo assembly. Error correction resulted in small but important improvements to the final assembly in terms of mapping statistics and core eukaryotic genes representation. The error-corrected de novo assembly resulted in 115,654 contigs after redundancy clustering. 41,667 assembled contigs were homologous to sequences from NCBI's non-redundant protein and UniProt databases. We assigned Gene Ontology, KEGG Orthology, Pfam protein domain terms and predicted protein-coding sequences to > 36,000 contigs. The final transcriptome dataset generated here provides functional information for 18,319 unique proteins, comprising at least 11,355 expressed genes. Furthermore, we identified 9,739 orthologs to P. miniata proteins, evaluated our annotation pipeline and generated a list of 150 candidate genes for responses to several environmental stressors that may be important for adaptation of A. amurensis in the invasive range. Our study has produced a large set of A. amurensis RNA contigs with functional annotations that can serve as a resource for future comparisons to other echinoderm transcriptomes and gene expression studies. Our data can be used to study the genetic basis of adaptive change and other important evolutionary processes during a successful invasion.
[Mh] Termos MeSH primário: Asterias
Regulação da Expressão Gênica/fisiologia
Espécies Introduzidas
RNA
Transcriptoma/fisiologia
[Mh] Termos MeSH secundário: Animais
Asterias/genética
Asterias/metabolismo
Austrália
Larva/genética
Larva/metabolismo
RNA/biossíntese
RNA/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
63231-63-0 (RNA)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170412
[Lr] Data última revisão:
170412
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0142003



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