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Pesquisa : B01.050.500.408.765.075 [Categoria DeCS]
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[PMID]:28545388
[Au] Autor:Rashid MH; Sadik G; Alam AK; Tanaka T
[Ad] Endereço:Institute of Biological Science, University of Rajshahi, Rajshahi, 6205, Bangladesh.
[Ti] Título:Chemical and structural characterization of α-N-acetylgalactosaminidase I and II from starfish, asterina amurensis.
[So] Source:BMC Biochem;18(1):9, 2017 May 25.
[Is] ISSN:1471-2091
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The marine invertebrate starfish was found to contain a novel α-N-acetylgalactosaminidase, α-GalNAcase II, which catalyzes removal of terminal α-N-acetylgalactosamine (α-GalNAc), in addition to a typical α-N-acetylgalactosaminidase, α-GalNAcase I, which catalyzes removal of terminal α-N-acetylgalactosamine (α-GalNAc) and, to a lesser extent, galactose. The interrelationship between α-GalNAcase I and α-GalNAcase II and the molecular basis of their differences in substrate specificity remain unknown. RESULTS: Chemical and structural comparisons between α-GalNAcase I and II using immunostaining, N-terminal amino acid sequencing and peptide analysis showed high homology to each other and also to other glycoside hydrolase family (GHF) 27 members. The amino acid sequence of peptides showed conserved residues at the active site as seen in typical α-GalNAcase. Some substitutions of conserved amino acid residues were found in α-GalNAcase II that were located near catalytic site. Among them G171 and A173, in place of C171 and W173, respectively in α-GalNAcase were identified to be responsible for lacking intrinsic α-galactosidase activity of α-GalNAcase II. Chemical modifications supported the presence of serine, aspartate and tryptophan as active site residues. Two tryptophan residues (W16 and W173) were involved in α-galactosidase activity, and one (W16) of them was involved in α-GalNAcase activity. CONCLUSIONS: The results suggested that α-GalNAcase I and II are closely related with respect to primary and higher order structure and that their structural differences are responsible for difference in substrate specificities.
[Mh] Termos MeSH primário: Asterina/enzimologia
alfa-N-Acetilgalactosaminidase/química
[Mh] Termos MeSH secundário: Animais
Domínio Catalítico
Dados de Sequência Molecular
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
alfa-Galactosidase/metabolismo
alfa-N-Acetilgalactosaminidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.2.1.22 (alpha-Galactosidase); EC 3.2.1.49 (alpha-N-Acetylgalactosaminidase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.1186/s12858-017-0085-1


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[PMID]:27838378
[Au] Autor:Yamamoto K; Kiyomoto M; Katayama H; Mita M
[Ad] Endereço:Department of Biology, Faculty of Education and Integrated Sciences, Center for Advanced Biomedical Sciences, Waseda University, Wakamatsucho 2-2, Shinjuku-ku, Tokyo 162-8480, Japan.
[Ti] Título:Radioimmunoassay of relaxin-like gonad-stimulating peptide in the starfish Patiria (=Asterina) pectinifera.
[So] Source:Gen Comp Endocrinol;243:84-88, 2017 Mar 01.
[Is] ISSN:1095-6840
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A relaxin-like gonad-stimulating peptide (RGP) from starfish Patiria (=Asterina) pectinifera is the first identified invertebrate gonadotropin for final gamete maturation. An antiserum against P. pectinifera RGP (PpeRGP) was produced by immunizing rabbits with a PpeRGP sulfanyl-polyethylene glycol derivative conjugated with keyhole limpet hemocyanin (KLH) as the antigen. The antiserum was used for the development of a specific and sensitive radioimmunoassay (RIA) for the measurement of RGP. In binding experiments using radioiodinated PpeRGP and antiserum against PpeRGP, a displacement curve was obtained using radioinert PpeRGP. The sensitivity of the RIA, defined as the amount of PpeRGP that significantly decreased the counts by 2 SD from the 100% bound point, averaged 0.040±0.002pmol PpeRGP per 100µl assay buffer (0.40±0.02nM) in 10 assays. Intra-assay and inter-assay coefficients of variation were 6.1% and 2.7%, respectively. Serial dilution of whole homogenates from the radial nerve cords and circumoral nerve-rings of P. pectinifera produced displacement curves parallel to the PpeRGP standard. Thus, the amounts of PpeRGP were determined as 1.54±0.09pmol/mg wet weight of radial nerves and 0.87±0.04pmol/mg wet weight of nerve-rings, respectively. On contrary, pyloric stomach, pyloric caeca, tube-feet, ovaries, testes, and ovarian follicle cells did not react in the RIA system. Furthermore, the A- and B-chains of PpeRGP, Asterias amurensis RGP, bovine insulin, and human relaxin did not show cross-reactivity in the RIA. These results strongly suggest that the RIA system is a highly specific and sensitive with respect to PpeRGP.
[Mh] Termos MeSH primário: Asterina/metabolismo
Gônadas/metabolismo
Hormônios de Invertebrado/metabolismo
Fragmentos de Peptídeos/metabolismo
Radioimunoensaio/métodos
Relaxina/metabolismo
[Mh] Termos MeSH secundário: Animais
Asterina/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Invertebrate Hormones); 0 (Peptide Fragments); 9002-69-1 (Relaxin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161114
[St] Status:MEDLINE


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[PMID]:27580613
[Au] Autor:Lim JE; Sung JK; Sarkar B; Wang H; Hashimoto Y; Tsang DC; Ok YS
[Ad] Endereço:Korea Biochar Research Center, Kangwon National University, Chuncheon, 24341, Korea.
[Ti] Título:Impact of natural and calcined starfish (Asterina pectinifera) on the stabilization of Pb, Zn and As in contaminated agricultural soil.
[So] Source:Environ Geochem Health;39(2):431-441, 2017 Apr.
[Is] ISSN:1573-2983
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Metal stabilization using soil amendments is an extensively applied, economically viable and environmentally friendly remediation technique. The stabilization of Pb, Zn and As in contaminated soils was evaluated using natural starfish (NSF) and calcined starfish (CSF) wastes at different application rates (0, 2.5, 5.0 and 10.0 wt%). An incubation study was conducted over 14 months, and the efficiency of stabilization for Pb, Zn and As in soil was evaluated by the toxicity characteristic leaching procedure (TCLP) test. The TCLP-extractable Pb was reduced by 76.3-100 and 91.2-100 % in soil treated with NSF and CSF, respectively. The TCLP-extractable Zn was also reduced by 89.8-100 and 93.2-100 % in soil treated with NSF and CSF, respectively. These reductions could be associated with the increased metal adsorption and the formation of insoluble metal precipitates due to increased soil pH following application of the amendments. However, the TCLP-extractable As was increased in the soil treated with NSF, possibly due to the competitive adsorption of phosphorous. In contrast, the TCLP-extractable As in the 10 % CSF treatment was not detectable because insoluble Ca-As compounds might be formed at high pH values. Thermodynamic modeling by visual MINTEQ predicted the formation of ettringite (Ca Al (SO ) (OH) ·26H O) and portlandite (Ca(OH) ) in the 10 % CSF-treated soil, while SEM-EDS analysis confirmed the needle-like structure of ettringite in which Pb was incorporated and stabilized in the 10 % CSF treatment.
[Mh] Termos MeSH primário: Asterina
Recuperação e Remediação Ambiental/métodos
Poluentes do Solo/análise
[Mh] Termos MeSH secundário: Animais
Arsênico/análise
Arsênico/química
Asterina/química
Concentração de Íons de Hidrogênio
Chumbo/análise
Chumbo/química
Microscopia Eletrônica de Varredura
Modelos Teóricos
República da Coreia
Poluentes do Solo/química
Poluentes do Solo/toxicidade
Termodinâmica
Testes de Toxicidade/métodos
Zinco/análise
Zinco/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil Pollutants); 2P299V784P (Lead); J41CSQ7QDS (Zinc); N712M78A8G (Arsenic)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160902
[St] Status:MEDLINE
[do] DOI:10.1007/s10653-016-9867-4


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[PMID]:27692029
[Au] Autor:Takahashi I; Kyozuka K
[Ad] Endereço:Research Center for Marine Biology,Asamushi,Graduate School of Life Science,Tohoku University,Asamushi,Aomori 039-3501,Japan.
[Ti] Título:Development of Ca2+-release mechanisms during oocyte maturation of the starfish Asterina pectinifera.
[So] Source:Zygote;24(6):857-868, 2016 Dec.
[Is] ISSN:1469-8730
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An important step for successful fertilization and further development is the increase in intracellular Ca2+ in the activated oocyte. It has been known that starfish oocytes become increasingly sensitive to inositol 1,4,5-trisphosphate (IP3) during meiotic maturation to exhibit highly efficient IP3-induced Ca2+ release (IICR) by the time of germinal vesicle breakdown (GVBD). However, we noted that the peak level of intracellular Ca2+ increase after insemination is already high in the maturing oocytes before GVBD. Using maturing oocytes before GVBD, we investigated Ca2+ release mechanisms other than IICR. We report here that Ca2+-release mechanisms dependent on nicotinic acid adenine dinucleotide phosphate (NAADP) and nicotinamide adenine dinucleotide (NADP), the precursor of NAADP, became functional prior to the development of IICR mechanisms. As with IP3, but unlike NAADP, the Ca2+ stores responsive to NADP are sensitized during the meiotic maturation induced by 1-methyladenine (1-MA). This suggests that the process may represent a physiological response to the maturation hormone. NADP-dependent Ca2+ release in immature oocytes, however, did not induce oocyte maturation by itself, but was enhanced by the conditions mimicking the increases of intracellular Ca2+ and pH that take place in the maturing oocytes of starfish.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Oócitos/fisiologia
Interações Espermatozoide-Óvulo
[Mh] Termos MeSH secundário: Adenina/análogos & derivados
Adenina/farmacologia
Animais
Asterina
Ionóforos de Cálcio/farmacologia
Citosol/metabolismo
Feminino
Fertilização In Vitro
Heparina/farmacologia
Técnicas de Maturação in Vitro de Oócitos
Inositol 1,4,5-Trifosfato/metabolismo
Ionomicina/farmacologia
Masculino
NADP/análogos & derivados
NADP/metabolismo
NADP/farmacologia
Oócitos/efeitos dos fármacos
Oócitos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium Ionophores); 5142-22-3 (1-methyladenine); 53-59-8 (NADP); 5502-96-5 (NAADP); 56092-81-0 (Ionomycin); 85166-31-0 (Inositol 1,4,5-Trisphosphate); 9005-49-6 (Heparin); JAC85A2161 (Adenine); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161004
[St] Status:MEDLINE


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[PMID]:26166482
[Au] Autor:Haraguchi S; Ikeda N; Abe M; Tsutsui K; Mita M
[Ad] Endereço:Department of Biology, Faculty of Education, Tokyo Gakugei University, Nukuikita-machi 4-1-1, Koganei-shi, Tokyo 184-8501, Japan; Laboratory of Integrative Brain Sciences, Department of Biology and Center for Medical Life Science, Waseda University, Wakamatsucho 2-2, Shinjuku-ku, Tokyo 162-8480, Jap
[Ti] Título:Nucleotide sequence and expression of relaxin-like gonad-stimulating peptide gene in starfish Asterina pectinifera.
[So] Source:Gen Comp Endocrinol;227:115-9, 2016 Feb 01.
[Is] ISSN:1095-6840
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Starfish gonad-stimulating substance (GSS) is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to gonadotropins in vertebrates. Because GSS belongs to the relaxin-like peptide family, we propose renaming for starfish gonadotropic hormone as relaxin-like gonad-stimulating peptide (RGP). This study examined the primary structure and expression regulation of the RGP gene in starfish Asterina pectinifera. RGP consisted of 3896 base pairs (bp) divided over two exons, exon 1 of 208 bp and exon 2 of 2277 bp, and one intron of 1411 bp. Promoter sequences, CAAT and TATA boxes, were present in the 5'-upstream region of the coding DNA sequence of RGP. The transcript was 2485 bases (b) in length. The AAUAAA polyadenylation signal was found in 3'-untranslated region over 2kb away from the stop codon. This showed that only 14% of the RGP mRNA was translated into the peptide, because a size of the open-reading frame was 351 b. Furthermore, an analysis by using real-time quantitative PCR with specific primers for RGP showed that mRNA of RGP was expressed at high levels in the radial nerves. Expression was also observed in the cardiac stomachs, although the level was low, and trace levels were detected in the gonads, pyloric caeca and tube feet. This result suggests that the RGP gene is transcribed mainly in the radial nerves of A. pectinifera.
[Mh] Termos MeSH primário: Asterina/metabolismo
Gônadas/metabolismo
Hormônios de Invertebrado/metabolismo
Neuropeptídeos/metabolismo
Relaxina/metabolismo
[Mh] Termos MeSH secundário: Animais
Asterina/genética
Sequência de Bases
Hormônios de Invertebrado/genética
Neuropeptídeos/genética
Ligação Proteica
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Relaxina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (GSS protein, Asteroidea); 0 (Invertebrate Hormones); 0 (Neuropeptides); 0 (RNA, Messenger); 9002-69-1 (Relaxin)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150714
[St] Status:MEDLINE


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[PMID]:25459377
[Au] Autor:Kalachev AV
[Ad] Endereço:AV Zhirmunsky Institute of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, 17 Palchevskogo str, Vladivostok 690041, Russia. akalachev@imb.dvo.ru
[Ti] Título:Reinvestigation of epithelial lining of the genital coelomic sinus in asteroids. An ultrastructural study.
[So] Source:Tissue Cell;46(6):540-5, 2014 Dec.
[Is] ISSN:1532-3072
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:Ultrastructural study of gonadal muscles in sea star, Asterina pectinifera, showed that myoepithelial cells were located only in the epithelial lining of the genital coelomic sinus. No myoepithelial cells were found in the visceral peritoneal epithelium or within connective tissue layer of the outer sac. Morphology of the myoepithelial cells in gonads of A. pectinifera varies during the reproductive cycle. During the gametogenic phase of the reproductive cycle, the myoepithelial cells get an elongated, spindle-like shape having a length of 20­30 m. In prespawning gonads, many of the myoepithelial cells form cytoplasmic extensions of 3­5 m in length, filled with myofilaments and penetrating into the underlying connective tissue of the outer sac or haemal sinus. Besides, myoepithelial cells, simultaneously anchored in the inner and outer sacs, were also observed. These changes result in development of more elaborated musculature and increase in contractility of the gonadal wall in prespawning gonads as compared to that during other stages of the reproductive cycle.
[Mh] Termos MeSH primário: Asterina/ultraestrutura
Células Epiteliais/ultraestrutura
Gônadas/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Tecido Conjuntivo/ultraestrutura
Microscopia Eletrônica
Músculos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1507
[Cu] Atualização por classe:150112
[Lr] Data última revisão:
150112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


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[PMID]:25226153
[Au] Autor:Cheatle Jarvela AM; Hinman V
[Ad] Endereço:Department of Biological Sciences, Carnegie Mellon University.
[Ti] Título:A method for microinjection of Patiria miniata zygotes.
[So] Source:J Vis Exp;(91):e51913, 2014 Sep 01.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Echinoderms have long been a favorite model system for studies of reproduction and development, and more recently for the study of gene regulation and evolution of developmental processes. The sea star, Patiria miniata, is gaining prevalence as a model system for these types of studies which were previously performed almost exclusively in the sea urchins, Strongylocentrotus purpuratus and Lytechinus variegatus. An advantage of these model systems is the ease of producing modified embryos in which a particular gene is up or downregulated, labeling a group of cells, or introducing a reporter gene. A single microinjection method is capable of creating a wide variety of such modified embryos. Here, we present a method for obtaining gametes from P. miniata, producing zygotes, and introducing perturbing reagents via microinjection. Healthy morphant embryos are subsequently isolated for quantitative and qualitative studies of gene function. The availability of genome and transcriptome data for this organism has increased the types of studies that are performed and the ease of executing them.
[Mh] Termos MeSH primário: Asterina/fisiologia
Biologia do Desenvolvimento/métodos
Modelos Animais
Zigoto/fisiologia
[Mh] Termos MeSH secundário: Animais
Asterina/genética
Feminino
Regulação da Expressão Gênica no Desenvolvimento
Masculino
Microinjeções
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; VIDEO-AUDIO MEDIA
[Em] Mês de entrada:1412
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140917
[St] Status:MEDLINE
[do] DOI:10.3791/51913


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[PMID]:24977934
[Au] Autor:Choi JH; Sapkota K; Kim S; Kim SJ
[Ad] Endereço:Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Chosun University, Gwangju 501-759, Republic of Korea.
[Ti] Título:Starase: A bi-functional fibrinolytic protease from hepatic caeca of Asterina pectinifera displays antithrombotic potential.
[So] Source:Biochimie;105:45-57, 2014 Oct.
[Is] ISSN:1638-6183
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:A bi-functional fibrinolytic serine protease, Starase exhibiting thrombolytic potency was purified from hepatic caeca of Asterina pectinifera. Starase showed a single band of approximately 48 kDa by SDS-PAGE and fibrin zymography. The N-terminal sequence of Starase was AIPTEFDARTKKHNN, which does not match with any known fibrinolytic enzyme. Starase had optimum amidolytic activity at 50 °C and pH 8.0 and the activity was inhibited by PMSF and APMSF. Starase showed the highest specificity toward the substrate H-D-Val-Leu-Lys-pNA for plasmin followed by pyroGlu-Gly-Arg-pNA for urokinase. The apparent Km and Vmax values of Starase toward a chromogenic substrate for plasmin H-D-Val-Leu-Lys-pNA were determined as 1.37 mM and 6.8 mM/min/mg respectively. The fibrinolytic activity of Starase by fibrin plate assay displayed that it could not only directly degrade fibrin clot but also activate plasminogen. Starase showed a strong fibrinogenolytic activity, cleaving all three major chains of fibrinogen rapidly. In addition, Starase with more than 1 µg could cleave extracellular matrix component type VII collagen, and plasma proteins such as bovine albumin and bovine gamma globulin. It could also inhibit factor Xa and thrombin activity. Starase at a dose of 0.8 mg/kg was devoid of hemorrhagic activity and it demonstrated antithrombotic effect in three animal models; FeCl2-induced carotid arterial thrombus model, carrageenan-induced tail thrombosis model and collagen and epinephrine induced pulmonary thromboembolism mice model. These results suggest that Starase has the potential to be a potent thrombolytic agent due to its bi-functional properties (containing both direct-acting and plasminogen-activating activities) and lack of hemorrhagic activity. Although Starase might interfere with the normal composition of the plasma proteins, it may be used not only for the treatment and prevention of thrombosis, but also in a number of biomedical applications.
[Mh] Termos MeSH primário: Asterina/enzimologia
Fibrinolíticos/química
Serina Proteases/química
Trombose/tratamento farmacológico
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Asterina/química
Colágeno Tipo VII/química
Colágeno Tipo VII/metabolismo
Fibrina/metabolismo
Fibrinogênio/metabolismo
Fibrinolíticos/administração & dosagem
Fibrinolíticos/metabolismo
Seres Humanos
Camundongos
Plasminogênio/química
Plasminogênio/metabolismo
Serina Proteases/administração & dosagem
Serina Proteases/isolamento & purificação
Serina Proteases/metabolismo
Especificidade por Substrato
Terapia Trombolítica
Trombose/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Collagen Type VII); 0 (Fibrinolytic Agents); 9001-31-4 (Fibrin); 9001-32-5 (Fibrinogen); 9001-91-6 (Plasminogen); EC 3.4.- (Serine Proteases)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:140913
[Lr] Data última revisão:
140913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140701
[St] Status:MEDLINE


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[PMID]:24929230
[Au] Autor:Mita M; Haraguchi S; Watanabe M; Takeshige Y; Yamamoto K; Tsutsui K
[Ad] Endereço:Department of Biology, Faculty of Education, Tokyo Gakugei University, Nukuikita-machi 4-1-1, Koganei-shi, Tokyo 184-8501, Japan. Electronic address: bio-mita@u-gakugei.ac.jp.
[Ti] Título:Involvement of Gαs-proteins in the action of relaxin-like gonad-stimulating substance on starfish ovarian follicle cells.
[So] Source:Gen Comp Endocrinol;205:80-7, 2014 Sep 01.
[Is] ISSN:1095-6840
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gonad-stimulating substance (GSS) in starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to gonadotropins in vertebrates. In breeding season (stage V), GSS stimulates oocyte maturation to induce 1-methyladenine (1-MeAde) by ovarian follicle cells. The hormonal action of GSS is mediated through the activation of its receptor, G-proteins and adenylyl cyclase. It has been reported that GSS fails to induce 1-MeAde and cyclic AMP (cAMP) production in follicle cells of ovaries during oogenesis (stage IV). This study examined the regulatory mechanism how ovarian follicle cells acquire the potential to respond to GSS by producing 1-MeAde and cAMP. Because the failure of GSS action was due to G-proteins of follicle cells, the molecular structures of Gαs, Gαi, Gαq and Gß were identified in follicle cells of starfish Asterina pectinifera. The cDNA sequences of Gαs, Gαi, Gαq and Gß consisted of ORFs encoding 379, 354, 353 and 353 amino acids. The expression levels of Gαs were extremely low in follicle cells at stage IV, whereas the mRNA levels increased markedly in stage V. On contrary, the mRNA levels of Gαi were almost constant regardless of stage IV and V. These findings strongly suggest that de novo synthesis of Gαs-proteins is contributed to the action of GSS on follicle cells to produce 1-MeAde and cAMP.
[Mh] Termos MeSH primário: Asterina/metabolismo
Subunidades alfa Gs de Proteínas de Ligação ao GTP/metabolismo
Hormônios de Invertebrado/farmacologia
Neuropeptídeos/farmacologia
Folículo Ovariano/citologia
Folículo Ovariano/metabolismo
Relaxina/metabolismo
[Mh] Termos MeSH secundário: Adenina/análogos & derivados
Adenina/biossíntese
Sequência de Aminoácidos
Animais
Asterina/efeitos dos fármacos
Sítios de Ligação
Western Blotting
Feminino
Subunidades alfa Gs de Proteínas de Ligação ao GTP/química
Subunidades alfa Gs de Proteínas de Ligação ao GTP/genética
Seres Humanos
Hormônios de Invertebrado/metabolismo
Cinética
Dados de Sequência Molecular
Oócitos/citologia
Oócitos/efeitos dos fármacos
Folículo Ovariano/efeitos dos fármacos
Filogenia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (GSS protein, Asteroidea); 0 (Invertebrate Hormones); 0 (Neuropeptides); 0 (RNA, Messenger); 5142-22-3 (1-methyladenine); 9002-69-1 (Relaxin); EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gs); JAC85A2161 (Adenine)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:140929
[Lr] Data última revisão:
140929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140615
[St] Status:MEDLINE


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[PMID]:24616226
[Au] Autor:Okumura E; Morita A; Wakai M; Mochida S; Hara M; Kishimoto T
[Ad] Endereço:Laboratory of Cell and Developmental Biology, Graduate School of Bioscience, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
[Ti] Título:Cyclin B-Cdk1 inhibits protein phosphatase PP2A-B55 via a Greatwall kinase-independent mechanism.
[So] Source:J Cell Biol;204(6):881-9, 2014 Mar 17.
[Is] ISSN:1540-8140
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Entry into M phase is governed by cyclin B-Cdk1, which undergoes both an initial activation and subsequent autoregulatory activation. A key part of the autoregulatory activation is the cyclin B-Cdk1-dependent inhibition of the protein phosphatase 2A (PP2A)-B55, which antagonizes cyclin B-Cdk1. Greatwall kinase (Gwl) is believed to be essential for the autoregulatory activation because Gwl is activated downstream of cyclin B-Cdk1 to phosphorylate and activate α-endosulfine (Ensa)/Arpp19, an inhibitor of PP2A-B55. However, cyclin B-Cdk1 becomes fully activated in some conditions lacking Gwl, yet how this is accomplished remains unclear. We show here that cyclin B-Cdk1 can directly phosphorylate Arpp19 on a different conserved site, resulting in inhibition of PP2A-B55. Importantly, this novel bypass is sufficient for cyclin B-Cdk1 autoregulatory activation. Gwl-dependent phosphorylation of Arpp19 is nonetheless necessary for downstream mitotic progression because chromosomes fail to segregate properly in the absence of Gwl. Such a biphasic regulation of Arpp19 results in different levels of PP2A-B55 inhibition and hence might govern its different cellular roles.
[Mh] Termos MeSH primário: Asterina/enzimologia
Proteína Quinase CDC2/metabolismo
Ciclina B/metabolismo
Proteína Fosfatase 2/metabolismo
[Mh] Termos MeSH secundário: Animais
Asterina/citologia
Células Cultivadas
Segregação de Cromossomos
Ativação Enzimática
Meiose
Fosfoproteínas/metabolismo
Fosforilação
Processamento de Proteína Pós-Traducional
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cyclin B); 0 (Phosphoproteins); 0 (cyclic AMP-regulated phosphoprotein 19); EC 2.7.11.22 (CDC2 Protein Kinase); EC 3.1.3.16 (Protein Phosphatase 2)
[Em] Mês de entrada:1405
[Cu] Atualização por classe:150514
[Lr] Data última revisão:
150514
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140312
[St] Status:MEDLINE
[do] DOI:10.1083/jcb.201307160



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