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[PMID]:29183362
[Au] Autor:Mulder C; Maas R
[Ad] Endereço:National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands. christian.mulder@rivm.nl.
[Ti] Título:Unifying the functional diversity in natural and cultivated soils using the overall body-mass distribution of nematodes.
[So] Source:BMC Ecol;17(1):36, 2017 Nov 28.
[Is] ISSN:1472-6785
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Sustainable use of our soils is a key goal for environmental protection. As many ecosystem services are supported belowground at different trophic levels by nematodes, soil nematodes are expected to provide objective metrics for biological quality to integrate physical and chemical soil variables. Trait measurements of body mass carried out at the individual level can in this way be correlated with environmental properties that influence the performance of soil biota. RESULTS: Soil samples were collected across 200 sites (4 soil types and 5 land-use types resulting in 9 combinations) during a long-term monitoring programme in the Netherlands and the functional diversity of nematode communities was investigated. Using three commonly used functional diversity indices applicable to single traits (Divergence, Evenness and Richness), a unified index of overall body-mass distribution is proposed to better illustrate the application of functional metrics as a descriptor of land use. Effects of land use and soil chemistry on the functional diversity of nematodes were demonstrated and a combination of environmental factors accounts for the low functional value of Scots Pine forest soils in comparison to the high functional value of heathland soils, whereas human factors account for the low functional and chemical values of arable fields. CONCLUSIONS: These findings show an unexpected high functional vulnerability of nematodes inhabiting clay-rich soils in comparison to sandy soils and support the notion that soil C:N ratio is a major driver of biodiversity. The higher the C:N ratio, the higher the overall diversity, as soil nematodes cope better with nutrient-poor agroecosystems under less intense fertilization. A trait-based way focusing on size distribution of nematodes is proposed to maintain environmental health by monitoring the overall diversity in soil biota, keeping agriculture and forestry sustainable.
[Mh] Termos MeSH primário: Biodiversidade
Nematoides/fisiologia
Solo/química
[Mh] Termos MeSH secundário: Animais
Tamanho Corporal
Meio Ambiente
Análise Multivariada
Países Baixos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1186/s12898-017-0145-9


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[PMID]:29256428
[Au] Autor:Morell M; Lehnert K; IJsseldijk LL; Raverty SA; Wohlsein P; Gröne A; André M; Siebert U; Shadwick RE
[Ad] Endereço:Zoology Department, University of British Columbia, Vancouver, British Columbia V6T1Z4, Canada.
[Ti] Título:Parasites in the inner ear of harbour porpoise: cases from the North and Baltic Seas.
[So] Source:Dis Aquat Organ;127(1):57-63, 2017 Dec 19.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Peribullar sinuses of harbour porpoises Phocoena phocoena are parasitized with high prevalence by the nematode Stenurus minor. The effect of S. minor on the hearing ability of this species is still undetermined. Here, we review the occurrence of S. minor in the inner ear of harbour porpoises recovered from strandings in the North and Baltic Seas. In particular, we present the results from ears collected in German and Danish waters from 2002 to 2016 and from Dutch waters from 2010 to 2016. While the prevalence of S. minor in pterygoid and peribullar sinuses and tympanic cavity was high in harbour porpoises (66.67% in our cases), its prevalence in the cochlea was rare. Only 1 case out of 129 analysed by either histology, electron microscopy or immunofluorescence showed the presence of a nematode parasite morphologically consistent with S. minor at the most basal portion of the right cochlea. This individual also had severe haemorrhage along the right cochlear spiral, which was likely caused by ectopic S. minor migration. Although this animal might have had impaired hearing in the right ear, it was otherwise in good body condition with evidence of recent feeding. These findings highlight the need to study the effect of parasites on hearing, and other pathological changes that might impair appropriate processing of acoustic information.
[Mh] Termos MeSH primário: Doenças do Labirinto/veterinária
Infecções por Nematoides/veterinária
Phocoena/parasitologia
[Mh] Termos MeSH secundário: Animais
Orelha Interna/parasitologia
Orelha Interna/ultraestrutura
Doenças do Labirinto/epidemiologia
Doenças do Labirinto/parasitologia
Nematoides/classificação
Nematoides/isolamento & purificação
Infecções por Nematoides/epidemiologia
Infecções por Nematoides/parasitologia
Mar do Norte/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.3354/dao03178


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[PMID]:29335213
[Au] Autor:Sugawara A; Kubo M; Hirose T; Yahagi K; Tsunoda N; Noguchi Y; Nakashima T; Takahashi Y; Welz C; Mueller D; Mertens C; Koebberling J; Omura S; Sunazuka T
[Ad] Endereço:Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan; Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aza-Aoba, Aramaki, Aoba-ku, Sendai, 980-8578, Japan. Electronic address
[Ti] Título:Jietacins, azoxy antibiotics with potent nematocidal activity: Design, synthesis, and biological evaluation against parasitic nematodes.
[So] Source:Eur J Med Chem;145:524-538, 2018 Feb 10.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Jietacins, an azoxy antibiotic class of chemicals, were isolated from the culture broth of Streptomyces sp. KP-197. They have a unique structural motif, including a vinyl azoxy group and a long acyclic aliphatic chain, which is usually branched but non-branched in the case of jietacin C. During a drug discovery program, we found that jietacins display potent anthelmintic activity against parasitic nematodes and that jietacin A has a moderate or low acute toxicity (LD > 300 mg/kg) and no mutagenic potential in a mini Ames screen. This suggests that jietacins have potential for drug discovery research. In order to create a novel anthelmintic agent, we performed design, synthesis, and biological evaluation of jietacin derivatives against parasitic nematodes. Of these derivatives, we found that a fully synthesized simplified derivative exhibited better anthelmintic activity against three parasitic nematodes than natural jietacins. In addition, it had a better efficacy in vivo through oral administration against a mouse nematode. This indicated that the azoxy motif could prove useful as a template for anthelmintic discovery, possibly creating a class of anthelmintic with novel skeletons, a potential new mode of action, and providing further insight for rational drug design.
[Mh] Termos MeSH primário: Anti-Helmínticos/farmacologia
Antibacterianos/farmacologia
Compostos Azo/farmacologia
Desenho de Drogas
Nematoides/efeitos dos fármacos
Nippostrongylus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Anti-Helmínticos/administração & dosagem
Anti-Helmínticos/química
Antibacterianos/administração & dosagem
Antibacterianos/química
Compostos Azo/administração & dosagem
Compostos Azo/química
Relação Dose-Resposta a Droga
Camundongos
Estrutura Molecular
Testes de Sensibilidade Parasitária
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Anti-Bacterial Agents); 0 (Azo Compounds); 109766-61-2 (jietacin A)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE


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[PMID]:29278825
[Au] Autor:Di Azevedo MIN; Iñiguez AM
[Ad] Endereço:LABTRIP, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21045-900, Brazil.
[Ti] Título:Nematode parasites of commercially important fish from the southeast coast of Brazil: Morphological and genetic insight.
[So] Source:Int J Food Microbiol;267:29-41, 2018 02 21.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Studies of nematofauna of teleost fish from the Brazilian coast are relatively scarce and limited to identification based on morphology. The objective of the present study was to determine the diversity and prevalence of nematode parasites in teleost fish from the southeast Atlantic coast of Rio de Janeiro, through morphological, molecular, and ecological approaches. Parasites were collected from sixty specimens each of Genypterus brasiliensis, Micropogonias furnieri, and Mullus argentinae obtained in winters and summers of 2012­2014. Morphological and genetic characterization was conducted using light microscopy and the molecular targets 18S rDNA, ITS1, and mtDNA cox2. Nematodes identified in M. furnieri were Cucculanus genypteri (n = 1575, P = 98.3%) and Hysterothylacium deardorffoverstreetorum (s.l.) (n = 2, P = 3.3%); in G. brasiliensis were Dichelyne (Cucullanellus) sciaenidicola (n = 99, P = 33.3%), Cucculanus pulcherrimus (n = 45, P = 18.3%), Hysterothylacium deardorffoverstreetorum (s.l.) (n = 3, P = 5%), and Anisakis typica (n = 1, P = 1.7%); and, in M. argentinae, were H. deardorffoverstreetorum (s.l.) (n = 146, P = 48.3%), and Procamallanus (Spirocamallanus) halitrophus (n = 4, P = 6.7%). DNA sequence data of C. genypteri, C. pulcherrimus, D. (C.) sciaenidicola, and P. (S.) halitrophus were reported for the first time. New host records are M. argentinae for P. (S.) halitrophus, M. furnieri for A. typica, while H. deardorffoverstreetorum (s.l.) was found in all three fish species. Intestine showed significantly higher intensity than other sites, and no significant seasonal variation in parasitological indices was observed. Hysterothylacium specimens (n = 6) were found in fish muscle, potentially a public health concern.
[Mh] Termos MeSH primário: Doenças dos Peixes/parasitologia
Nematoides/genética
Infecções por Nematoides/parasitologia
Perciformes/parasitologia
[Mh] Termos MeSH secundário: Animais
Brasil
DNA Mitocondrial/genética
DNA Ribossômico/genética
Nematoides/classificação
Nematoides/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (DNA, Ribosomal)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171227
[St] Status:MEDLINE


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[PMID]:29364951
[Au] Autor:Puehringer-Sturmayr V; Wascher CAF; Loretto MC; Palme R; Stoewe M; Kotrschal K; Frigerio D
[Ad] Endereço:Core Facility Konrad Lorenz Forschungsstelle for Behaviour and Cognition, University of Vienna, Grünau im Almtal, Austria.
[Ti] Título:Seasonal differences of corticosterone metabolite concentrations and parasite burden in northern bald ibis (Geronticus eremita): The role of affiliative interactions.
[So] Source:PLoS One;13(1):e0191441, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The reproductive season is energetically costly as revealed by elevated glucocorticoid concentrations, constrained immune functions and an increased risk of infections. Social allies and affiliative interactions may buffer physiological stress responses and thereby alleviate associated effects. In the present study, we investigated the seasonal differences of immune reactive corticosterone metabolite concentrations, endoparasite burden (nematode eggs and coccidian oocysts) and affiliative interactions in northern bald ibis (Geronticus eremita), a critically endangered bird. In total, 43 individually marked focal animals from a free-ranging colony were investigated. The analyses included a description of initiated and received affiliative interactions, pair bond status as well as seasonal patterns of hormone and endoparasite levels. During the reproductive season, droppings contained parasite eggs more often and corticosterone metabolite levels were higher as compared to the period after reproduction. The excretion rate of endoparasite products was lower in paired individuals than in unpaired ones, but paired animals exhibited higher corticosterone metabolite concentrations than unpaired individuals. Furthermore, paired individuals initiated affiliative behaviour more frequently than unpaired ones. This suggests that the reproductive season influences the excretion patterns of endoparasite products and corticosterone metabolites and that affiliative interactions between pair partners may positively affect endoparasite burden during periods of elevated glucocorticoid levels. Being embedded in a pair bond may have a positive impact on individual immune system and parasite resistance.
[Mh] Termos MeSH primário: Aves/fisiologia
Aves/parasitologia
Corticosterona/metabolismo
[Mh] Termos MeSH secundário: Animais
Comportamento Animal/fisiologia
Aves/imunologia
Coccídios/isolamento & purificação
Coccídios/patogenicidade
Feminino
Masculino
Nematoides/isolamento & purificação
Nematoides/patogenicidade
Oocistos/isolamento & purificação
Oocistos/patogenicidade
Ligação do Par
Contagem de Ovos de Parasitas
Reprodução/fisiologia
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
W980KJ009P (Corticosterone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191441


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[PMID]:28466113
[Au] Autor:Acosta AA; González-Solís D; da Silva RJ
[Ad] Endereço:Departamento de Parasitologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Distrito de Rubião Júnior s/n, Botucatu, São Paulo, CEP 18618-970, Brazil.
[Ti] Título:Spinitectus aguapeiensis n. sp. (Nematoda: Cystidicolidae) from Pimelodella avanhandavae Eigenmann (Siluriformes: Heptapteridae) in the River Aguapeí, Upper Paraná River Basin, Brazil.
[So] Source:Syst Parasitol;94(6):649-656, 2017 07.
[Is] ISSN:1573-5192
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Nematodes belonging to Spinitectus Fourment, 1883 (Nematoda: Cystidicolidae) were found in the intestine of Pimelodella avanhandavae Eigenmann (Siluriformes: Heptapteridae) from the Aguapeí River, Brazil. They represent a new species, Spinitectus aguapeiensis n. sp., which differs morphologically from its congeners in the body length, the number of spinose rings, the location of the excretory pore, the number of precloacal papillae and the length of the spicules. The new species is the first South American species within the genus with a remarkably spirally coiled posterior extremity in males and the largest spicules. It is also the second species with the highest number of precloacal papillae and has unique shape of the small spicule. Spinitectus aguapeiensis n. sp. is the first helminth species found in P. avanhandavae, the fourth species of this genus recorded in the River Paraná Basin and the sixth species of Spinitectus in South America.
[Mh] Termos MeSH primário: Peixes-Gato/parasitologia
Nematoides/classificação
[Mh] Termos MeSH secundário: Animais
Brasil
Feminino
Intestinos/parasitologia
Masculino
Nematoides/anatomia & histologia
Rios
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1007/s11230-017-9729-1


  7 / 7853 MEDLINE  
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[PMID]:29401577
[Au] Autor:Ryss AY
[Ti] Título:The simplest "field" methods for extractin of nematodes from plants, wood, insects and soil, with additional description how to keep extracted nematodes alive for a long time.
[So] Source:Parazitologiia;51(1):57-67, 2017 Jan-Feb.
[Is] ISSN:0031-1847
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The simplest modification of the dynamic extraction method using cottonwool filter based on the Baermann funnel principle, is described. This modification excludes the funnel because a great share of Sticky worms attach to sloping walls of a funnel and thus do not reach the collector Eppendorf tube. But the main principle of the Baermann funnel is used, I. e. sinking down of actively moving heavy narrow bodies via wide holes of filter and thus separating the active worms from passive non-Brownian moving substrate particles, which do not pass the filter and remain above it. This principle is illustrated because it has never been described before. In the proposed modification any sloping walls in the extraction paths are excluded and thus the probability to attach sticky nemotodes to walls is also excluded; only cylindrical equipment with abrupt vertical walls is used; procedures are extremely simplified to be user-friendly for beginners: only filter (cotton pads), Eppendorf tubes, plastic glasses and narrow PVC tubing are applied. The new simplified modification allows one to collect nematodes by non-professional workers, e. g. in Polar expeditions without microscopic study of results. As an addition, an efficient method to maintain extracted nematodes alive is proposed, using the "effect of water film" in foam rubber inside the Eppendorf tube. To maintain nematodes alive during several months it is recommended to suppress bacteria via addition of 0.2-0.4% formaldehyde solution and then keep the tube with nematodes in a refrigerator.
[Mh] Termos MeSH primário: Desenho de Equipamento/instrumentação
Movimento/fisiologia
Nematoides/isolamento & purificação
Refrigeração/métodos
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
Fricção/fisiologia
Insetos/parasitologia
Plantas/parasitologia
Solo/parasitologia
Madeira/parasitologia
[Pt] Tipo de publicação:RESEARCH SUPPORT, NON-U.S. GOV'T; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180206
[St] Status:MEDLINE


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[PMID]:29262347
[Au] Autor:Harris NL; Loke P
[Ad] Endereço:Global Health Institute, École Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland. Electronic address: nicola.harris@epfl.ch.
[Ti] Título:Recent Advances in Type-2-Cell-Mediated Immunity: Insights from Helminth Infection.
[So] Source:Immunity;47(6):1024-1036, 2017 Dec 19.
[Is] ISSN:1097-4180
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Type-2-cell-mediated immune responses play a critical role in mediating both host-resistance and disease-tolerance mechanisms during helminth infections. Recently, type 2 cell responses have emerged as major regulators of tissue repair and metabolic homeostasis even under steady-state conditions. In this review, we consider how studies of helminth infection have contributed toward our expanding cellular and molecular understanding of type-2-cell-mediated immunity, as well as new areas such as the microbiome. By studying how these successful parasites form chronic infections without overt pathology, we are gaining additional insights into allergic and inflammatory diseases, as well as normal physiology.
[Mh] Termos MeSH primário: Helmintíase/imunologia
Imunidade Celular
Macrófagos/imunologia
Nematoides/imunologia
Células Th2/imunologia
Trematódeos/imunologia
[Mh] Termos MeSH secundário: Animais
Citocinas/genética
Citocinas/imunologia
Células Epiteliais/imunologia
Células Epiteliais/parasitologia
Regulação da Expressão Gênica/imunologia
Helmintíase/genética
Helmintíase/parasitologia
Homeostase/imunologia
Interações Hospedeiro-Parasita/imunologia
Seres Humanos
Macrófagos/parasitologia
Mastócitos/imunologia
Mastócitos/parasitologia
Microbiota/imunologia
Células Th2/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cytokines)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE


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[PMID]:29324824
[Au] Autor:Leary CJ; Ralicki HF; Laurencio D; Crocker-Buta S; Malone JH
[Ad] Endereço:University of Mississippi, Department of Biology, University, Mississippi, United States of America.
[Ti] Título:Assessing the links among environmental contaminants, endocrinology, and parasites to understand amphibian declines in montane regions of Costa Rica.
[So] Source:PLoS One;13(1):e0191183, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Amphibians inhabiting montane riparian zones in the Neotropics are particularly vulnerable to decline, but the reasons are poorly understood. Because environmental contaminants, endocrine disruption, and pathogens often figure prominently in amphibian declines it is imperative that we understand how these factors are potentially interrelated to affect montane populations. One possibility is that increased precipitation associated with global warming promotes the deposition of contaminants in montane regions. Increased exposure to contaminants, in turn, potentially elicits chronic elevations in circulating stress hormones that could contribute to montane population declines by compromising resistance to pathogens and/or production of sex steroids regulating reproduction. Here, we test this hypothesis by examining contaminant levels, stress and sex steroid levels, and nematode abundances in male drab treefrogs, Smilisca sordida, from lowland and montane populations in Costa Rica. We found no evidence that montane populations were more likely to possess contaminants (i.e., organochlorine, organophosphate and carbamate pesticides or benzidine and chlorophenoxy herbicides) than lowland populations. We also found no evidence of elevational differences in circulating levels of the stress hormone corticosterone, estradiol or progesterone. However, montane populations possessed lower androgen levels, hosted more nematode species, and had higher nematode abundances than lowland populations. Although these results suggested that nematodes contributed to lower androgens in montane populations, we were unable to detect a significant inverse relationship between nematode abundance and androgen level. Our results suggest that montane populations of this species are not at greater risk of exposure to contaminants or chronic stress, but implicate nematodes and compromised sex steroid levels as potential threats to montane populations.
[Mh] Termos MeSH primário: Anuros/parasitologia
Disruptores Endócrinos/toxicidade
Poluentes Ambientais/toxicidade
[Mh] Termos MeSH secundário: Animais
Anuros/sangue
Anuros/fisiologia
Corticosterona/sangue
Costa Rica
Disruptores Endócrinos/metabolismo
Glândulas Endócrinas/efeitos dos fármacos
Glândulas Endócrinas/fisiopatologia
Poluentes Ambientais/metabolismo
Aquecimento Global
Hormônios Esteroides Gonadais/sangue
Interações Hospedeiro-Parasita
Masculino
Nematoides/isolamento & purificação
Nematoides/patogenicidade
Dinâmica Populacional
Estresse Fisiológico
Clima Tropical/efeitos adversos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Endocrine Disruptors); 0 (Environmental Pollutants); 0 (Gonadal Steroid Hormones); W980KJ009P (Corticosterone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191183


  10 / 7853 MEDLINE  
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[PMID]:29300744
[Au] Autor:Santos JRP; Ndeve AD; Huynh BL; Matthews WC; Roberts PA
[Ad] Endereço:Department of Nematology, University of California, Riverside, California, United States of America.
[Ti] Título:QTL mapping and transcriptome analysis of cowpea reveals candidate genes for root-knot nematode resistance.
[So] Source:PLoS One;13(1):e0189185, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cowpea is one of the most important food and forage legumes in drier regions of the tropics and subtropics. However, cowpea yield worldwide is markedly below the known potential due to abiotic and biotic stresses, including parasitism by root-knot nematodes (Meloidogyne spp., RKN). Two resistance genes with dominant effect, Rk and Rk2, have been reported to provide resistance against RKN in cowpea. Despite their description and use in breeding for resistance to RKN and particularly genetic mapping of the Rk locus, the exact genes conferring resistance to RKN remain unknown. In the present work, QTL mapping using recombinant inbred line (RIL) population 524B x IT84S-2049 segregating for a newly mapped locus and analysis of the transcriptome changes in two cowpea near-isogenic lines (NIL) were used to identify candidate genes for Rk and the newly mapped locus. A major QTL, designated QRk-vu9.1, associated with resistance to Meloidogyne javanica reproduction, was detected and mapped on linkage group LG9 at position 13.37 cM using egg production data. Transcriptome analysis on resistant and susceptible NILs 3 and 9 days after inoculation revealed up-regulation of 109 and 98 genes and down-regulation of 110 and 89 genes, respectively, out of 19,922 unique genes mapped to the common bean reference genome. Among the differentially expressed genes, four and nine genes were found within the QRk-vu9.1 and QRk-vu11.1 QTL intervals, respectively. Six of these genes belong to the TIR-NBS-LRR family of resistance genes and three were upregulated at one or more time-points. Quantitative RT-PCR validated gene expression to be positively correlated with RNA-seq expression pattern for eight genes. Future functional analysis of these cowpea genes will enhance our understanding of Rk-mediated resistance and identify the specific gene responsible for the resistance.
[Mh] Termos MeSH primário: Genes de Plantas
Nematoides/patogenicidade
Locos de Características Quantitativas
Transcriptoma
Vigna/genética
[Mh] Termos MeSH secundário: Animais
Regulação da Expressão Gênica de Plantas
Genótipo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Análise de Sequência de RNA
Vigna/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180105
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189185



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