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| [PMID]: | 27677942 |
| [Au] Autor: | Zhao JY; Zhao WT; Ali AH; Chen HX; Li L |
| [Ad] Endereço: | Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, 050024 Shijiazhuang, Hebei Province, PR China. |
| [Ti] Título: | Morphological variability, ultrastructure and molecular characterisation of Hysterothylacium reliquens (Norris & Overstreet, 1975) (Nematoda: Raphidascarididae) from the oriental sole Brachirus orientalis (Bloch & Schneider) (Pleuronectiformes: Soleidae). |
| [So] Source: | Parasitol Int;66(1):831-838, 2017 Feb. | | [Is] ISSN: | 1873-0329 |
| [Cp] País de publicação: | Netherlands |
| [La] Idioma: | eng |
| [Ab] Resumo: | Hysterothylacium reliquens (Norris & Overstreet, 1975), a common ascaridoid nematode parasitic in the marine fishes in the Indian and Atlantic Oceans, has been reported to exhibit remarkable morphological variability in the lengths of body, oesophagus and spicules, and the number and arrangement of postcloacal papillae. In order to determine whether H. reliquens with a broad range of morphological variability in the above-mentioned respects, represents a complex of sibling species or a single species, and to evaluate if the specimens of H. reliquens collected from the Indian and Atlantic Oceans are the same species, numbers of H. reliquens collected from the oriental sole Brachirus orientalis (Bloch & Schneider) (Pleuronectiformes: Soleidae) in the Arabian Gulf (off Iraq) were characterised using molecular approaches by sequencing and analysing the ribosomal [large ribosomal DNA (28S) and internal transcribed spacer (ITS)] and mitochondrial [cytochrome c oxidase subunit 2 (cox2)] target regions, respectively. The results of molecular analysis strongly supported that: (i) the broad ranges of morphological variability in the above-mentioned respects in the newly collected specimens of H. reliquens, should be considered as intraspecific variation and the nematode material collected from B. orientalis in the Arabian Gulf represented a single species; (ii) the specimens of H. reliquens collected from the Indian and Atlantic Oceans belong to the same species, but they possibly represent different geographical populations. Moreover, the detailed morphology of the labial papillae, lateral alae, excretory pore, distal end of spicules, midventral precloacal papilla, phasmids and caudal papillae was revealed by SEM for the first time. The molecular and morphological data of H. reliquens obtained herein contribute to a more accurate diagnosis of this little known ascaridoid nematode. |
| [Mh] Termos MeSH primário: |
Infecções por Ascaridida/veterinária
Ascaridídios/genética
Ascaridídios/ultraestrutura
Doenças dos Peixes/parasitologia
Linguados/parasitologia
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| [Mh] Termos MeSH secundário: |
Animais
Ascaridídios/anatomia & histologia
Ascaridídios/classificação
Infecções por Ascaridida/parasitologia
DNA Espaçador Ribossômico
Doenças dos Peixes/diagnóstico
Genes de Protozoários/genética
Iraque
Microscopia Eletrônica de Varredura
Oceanos e Mares
Filogenia
RNA Ribossômico 28S
Análise de Sequência de DNA
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| [Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 28S) |
| [Em] Mês de entrada: | 1704 |
| [Cu] Atualização por classe: | 170417 |
[Lr] Data última revisão:
| 170417 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 161107 |
| [St] Status: | MEDLINE |
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