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Bittencourt, Vânia Rita Elias Pinheiro
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[PMID]:28655584
[Au] Autor:Tunholi VM; Tunholi-Alves VM; Monteiro CO; Silva LCD; Dolinski CM; Castro RN; Bittencourt VREP; Silva JPD; Freire Martins IV
[Ad] Endereço:Departamento de Medicina Veterinária, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo - UFES, Alegre, ES, Brazil. Electronic address: vinnytunholi@yahoo.com.br.
[Ti] Título:Biological, biochemical and histological features of Bradybaena similaris (Gastropoda: Pulmonata) infected by Heterorabditis indica (Rhabditida: Heterorhabditidae) strain LPP1.
[So] Source:Exp Parasitol;179:28-35, 2017 Aug.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated the possible biological, biochemical and histological changes in Bradybaena similaris(Gastropoda: Pulmonata) infected by Heterorhabditis indica (Rhabditida: Heterorhabditidae), strain LPP1. Two groups of 16 snails were formed: the control group (unexposed) and the treated group, which was exposed for three weeks to infective juveniles (J ) of H. indica LPP1. The experiment was conducted in duplicate, using a total of 64 snails. After the exposure period, the snails were dissected to collect the hemolymph and tissues, for evaluation of the physiological changes caused by the infection. The number of eggs laid/snail and the viability of these eggs were also assessed as indicators of the reproductive activity of B. similaris. Intense glycogenolysis was accompanied by a significant reduction (p < 0.05) in the glucose content of the hemolymph of the exposed snails, indicating that infection by H. indica induces breakdown of the host's glycemic homeostasis. Significant variations (p < 0.05) in the lactate dehydrogenase activity occurred together with changes in the concentration of pyruvic and lactic acid in the hemolymph of the infected B. similaris snails, corroborating the transition from aerobic to anaerobic metabolism in the hosts. These metabolic alterations reflect the parasitic castration process in this interface. The results suggest that the use of H. indica LPP1 is a potential alternative for biological control of B. similaris.
[Mh] Termos MeSH primário: Gastrópodes/parasitologia
Rhabditoidea/fisiologia
[Mh] Termos MeSH secundário: Animais
Estudos de Casos e Controles
Cromatografia Líquida de Alta Pressão
Galactanos/análise
Gastrópodes/anatomia & histologia
Gastrópodes/metabolismo
Glucose/metabolismo
Glicogênio/metabolismo
Hemolinfa/química
Hemolinfa/enzimologia
Hemolinfa/metabolismo
L-Lactato Desidrogenase/metabolismo
Ácido Láctico/análise
Mariposas/parasitologia
Ácido Pirúvico/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Galactans); 33X04XA5AT (Lactic Acid); 37208-43-8 (galactogen); 8558G7RUTR (Pyruvic Acid); 9005-79-2 (Glycogen); EC 1.1.1.27 (L-Lactate Dehydrogenase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE


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[PMID]:28640534
[Au] Autor:An R; Suri KS; Jurat-Fuentes JL; Grewal PS
[Ad] Endereço:Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, USA.
[Ti] Título:Dynamics of transcriptomic response to infection by the nematode Heterorhabditis bacteriophora and its bacterial symbiont Photorhabdus temperata in Heliothis virescens larvae.
[So] Source:Insect Mol Biol;26(5):584-600, 2017 Oct.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Entomopathogenic nematodes in the Heterorhabditis genus and their symbiotic Photorhabdus bacteria are important biocontrol agents of insect pests and models for the study of microbe-host interactions. In this work, we used larvae of the tobacco budworm (Heliothis virescens) as a model to study its defensive mechanisms against Heterorhabditis bacteriophora nematodes carrying symbiotic Photorhabdus temperata. We first determined time points of initial nematode entry and release of bacteria into the haemolymph to perform transcriptional analysis of insect gene expression during these steps in the infective process. RNA-Sequencing analyses were then performed to profile differential gene expression in the insect during nematode invasion, bacterial release and final steps of infection, relative to the untreated controls. Our results support the theory that insect immune response genes are induced upon nematode invasion, but the majority of these genes are suppressed upon the release of bacteria by the nematodes into the haemolymph. Overall, these findings provide information on the dynamics of the insect's response to a progressing infection by this entomopathogenic nematode-bacteria complex and facilitate development of Hel. virescens as a pest model for future functional studies of the key insect defence factors.
[Mh] Termos MeSH primário: Interações Hospedeiro-Parasita/imunologia
Mariposas/imunologia
Mariposas/metabolismo
Photorhabdus/fisiologia
Rhabditoidea/fisiologia
[Mh] Termos MeSH secundário: Animais
Perfilação da Expressão Gênica
Mariposas/genética
Reação em Cadeia da Polimerase em Tempo Real
Rhabditoidea/microbiologia
Análise de Sequência de RNA
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12321


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[PMID]:28395586
[Au] Autor:Darsouei R; Karimi J; Ghadamyari M; Hosseini M
[Ad] Endereço:Biocontrol and Insect Pathology Laboratory, Department of Plant Protection, School of Agriculture, Ferdowsi University of Mashhad, 91779-48974 Mashhad, Iran.
[Ti] Título:Differential Change Patterns of Main Antimicrobial Peptide Genes During Infection of Entomopathogenic Nematodes and Their Symbiotic Bacteria.
[So] Source:J Parasitol;103(4):349-358, 2017 Aug.
[Is] ISSN:1937-2345
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The expression of antimicrobial peptides (AMPs) as the main humoral defense reactions of insects during infection by entomopathogenic nematodes (EPNs) and their symbiont is addressed herein. Three AMPs, attacin, cecropin, and spodoptericin, were evaluated in the fifth instar larvae of Spodoptera exigua Hübner (beet armyworm) when challenged with Steinernema carpocapsae or Heterorhabditis bacteriophora. The results indicated that attacin was expressed to a greater extent than either cecropin or spodoptericin. While spodoptericin was expressed to a much lesser extent, this AMP was induced against Gram-positive bacteria, and thus not expressed after penetration of Xenorhabdus nematophila and Photorhabdus luminescens. Attacin and cecropin in the larvae treated with S. carpocapsae at 8 hr post-injection (PI) attained the maximum expression levels and were 138.42-fold and 65.84-fold greater than those of larvae infected with H. bacteriophora, respectively. Generally, the ability of H. bacteriophora to suppress attacin, cecropin, and spodoptericin was greater than that of S. carpocapsae. According to the results, the expression of AMPs by Sp. exigua larvae against S. carpocapsae was determined in the 4 statuses of monoxenic nematode, axenic nematode, live symbiotic bacterium, and dead symbiotic bacterium. The expression of attacin in larvae treated with a monoxenic nematode and live bacterium at 8 and 2 hr PI, respectively, were increased to the maximum amount. Live X. nematophila was the strongest agent for the suppression of attacin. The expression of cecropin against monoxenic nematodes and live symbiotic bacteria at 8 and 4 hr PI, respectively, reached the maximum amount while the expression levels of attacin and cecropin for axenic nematodes were lesser and stable. The results highlighted that the ability of P. luminescens in AMPs suppression was much more than X. nematophila. The results also showed that the effect of symbiotic bacterium in suppressing attacin and cecropin expression was greater than that of a monoxenic nematode; this result provided deep insight into the expression pattern parallels and fluctuations of the main AMPs during nematode infection.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/genética
Bactérias/metabolismo
Nematoides/metabolismo
Nematoides/microbiologia
[Mh] Termos MeSH secundário: Animais
Peptídeos Catiônicos Antimicrobianos/metabolismo
Bactérias/genética
Cecropinas/genética
Cecropinas/metabolismo
Feminino
Expressão Gênica
Proteínas de Insetos/genética
Proteínas de Insetos/metabolismo
Mariposas/parasitologia
Rabditídios/metabolismo
Rabditídios/microbiologia
Rhabditoidea/metabolismo
Rhabditoidea/microbiologia
Spodoptera/metabolismo
Spodoptera/microbiologia
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (Cecropins); 0 (Insect Proteins); 0 (attacin antibacterial protein, insect)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1645/16-162


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[PMID]:28246174
[Au] Autor:Park HB; Sampathkumar P; Perez CE; Lee JH; Tran J; Bonanno JB; Hallem EA; Almo SC; Crawford JM
[Ad] Endereço:From the Department of Chemistry, Yale University, New Haven, Connecticut 06520.
[Ti] Título:Stilbene epoxidation and detoxification in a -nematode symbiosis.
[So] Source:J Biol Chem;292(16):6680-6694, 2017 Apr 21.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Members of the gammaproteobacterial genus share mutualistic relationships with nematodes, and the pairs infect a wide swath of insect larvae. species produce a family of stilbenes, with two major components being 3,5-dihydroxy-4-isopropyl- -stilbene (compound 1) and its stilbene epoxide (compound 2). This family of molecules harbors antimicrobial and immunosuppressive activities, and its pathway is responsible for producing a nematode "food signal" involved in nematode development. However, stilbene epoxidation biosynthesis and its biological roles remain unknown. Here, we identified an orphan protein (Plu2236) from that catalyzes stilbene epoxidation. Structural, mutational, and biochemical analyses confirmed the enzyme adopts a fold common to FAD-dependent monooxygenases, contains a tightly bound FAD prosthetic group, and is required for the stereoselective epoxidation of compounds 1 and 2. The epoxidase gene was dispensable in a nematode-infective juvenile recovery assay, indicating the oxidized compound is not required for the food signal. The epoxide exhibited reduced cytotoxicity toward its producer, suggesting this may be a natural route for intracellular detoxification. In an insect infection model, we also observed two stilbene-derived metabolites that were dependent on the epoxidase. NMR, computational, and chemical degradation studies established their structures as new stilbene-l-proline conjugates, prolbenes A (compound 3) and B (compound 4). The prolbenes lacked immunosuppressive and antimicrobial activities compared with their stilbene substrates, suggesting a metabolite attenuation mechanism in the animal model. Collectively, our studies provide a structural view for stereoselective stilbene epoxidation and functionalization in an invertebrate animal infection model and provide new insights into stilbene cellular detoxification.
[Mh] Termos MeSH primário: Compostos de Epóxi/química
Photorhabdus/metabolismo
Rhabditoidea/microbiologia
Estilbenos/química
Simbiose
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/química
Produtos Biológicos/química
Catálise
Cromatografia Líquida de Alta Pressão
Cristalografia por Raios X
Análise Mutacional de DNA
Deleção de Genes
Ligações de Hidrogênio
Interações Hidrofóbicas e Hidrofílicas
Imunossupressores/química
Espectroscopia de Ressonância Magnética
Conformação Molecular
Mutação
Dobramento de Proteína
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Biological Products); 0 (Epoxy Compounds); 0 (Immunosuppressive Agents); 0 (Stilbenes)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.762542


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[PMID]:28062989
[Au] Autor:Ross JL; Haukeland S; Hatteland BA; Ivanova ES
[Ad] Endereço:Department of Conservation Ecology and Entomology, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. jenna.ross@abdn.ac.uk.
[Ti] Título:Angiostoma norvegicum n. sp. (Nematoda: Angiostomatidae) a parasite of arionid slugs in Norway.
[So] Source:Syst Parasitol;94(1):51-63, 2017 Jan.
[Is] ISSN:1573-5192
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Angiostoma norvegicum n. sp. (Angiostomatidae) is described from the oesophagus, crop and the buccal mass of five species of slugs of the family Arionidae, Arion vulgaris (Moquin-Tandon), Arion ater (L.), Arion fasciatus (Nilsson), Arion fuscus (Müller) and Arion rufus/Arion ater hybrid), collected throughout Norway. Angiostoma norvegicum n. sp. was found parasitising arionids at seven of the 30 sample sites examined (23.3%), and 9.9% of all Arion spp. were infected with this nematode. The new species is characterised by its large size (4.0-8.6 mm long) and in having: lateral alae; 6 + 6 papillae at the cephalic end; a large circular mouth aperture; a spacious stoma; a pharyngeal basal bulb without valvular apparatus; an excretory pore near the base of bulb; a distal part of posterior ovary always outstretched; an anterior ovary distally nearly always outstretched; a vulva situated anterior to mid-body; long, nearly straight spicules and a small gubernaculum; three circumcloacal papillae and caudal genital papillae (GP) arranged in a pattern 1+2/3+3 with GP 5 and GP 8 opened on dorsal side of narrow bursa not reaching tail tip; short conical tails in both sexes with tips supplied by 4 short, unequal denticles. Morphologically, A. norvegicum n. sp. is similar to Angiostoma limacis Dujardin, 1845, which diagnostic characteristics are given based on examination of specimens from Norway and the UK. Conversely, the phylogenetic analyses based on D2D3 large subunit (LSU) rRNA gene sequences performed in the present study did not support the morphological affinity of these two species. Phylogenetic analyses demonstrated that although Angiostoma spp. cluster together, A. norvegicum n. sp. forms a tight monophyletic clade with the milacid nematode parasites Angiostoma margaretae Ross, Malan & Ivanova, 2011 and Angiostoma milacis Ivanova & Wilson, 2009.
[Mh] Termos MeSH primário: Gastrópodes/parasitologia
Filogenia
Rhabditoidea/classificação
[Mh] Termos MeSH secundário: Animais
Noruega
RNA Ribossômico/genética
Rhabditoidea/anatomia & histologia
Rhabditoidea/genética
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE
[do] DOI:10.1007/s11230-016-9674-4


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[PMID]:28049427
[Au] Autor:Vadnal J; Ratnappan R; Keaney M; Kenney E; Eleftherianos I; O'Halloran D; Hawdon JM
[Ad] Endereço:Department of Biological Sciences, George Washington University, Science and Engineering Hall, suite 6000, 800 22nd Street NW, Washington DC, 20052, USA.
[Ti] Título:Identification of candidate infection genes from the model entomopathogenic nematode Heterorhabditis bacteriophora.
[So] Source:BMC Genomics;18(1):8, 2017 01 03.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Despite important progress in the field of innate immunity, our understanding of host immune responses to parasitic nematode infections lags behind that of responses to microbes. A limiting factor has been the obligate requirement for a vertebrate host which has hindered investigation of the parasitic nematode infective process. The nematode parasite Heterorhabditis bacteriophora offers great potential as a model to genetically dissect the process of infection. With its mutualistic Photorhabdus luminescens bacteria, H. bacteriophora invades multiple species of insects, which it kills and exploits as a food source for the development of several nematode generations. The ability to culture the life cycle of H. bacteriophora on plates growing the bacterial symbiont makes it a very exciting model of parasitic infection that can be used to unlock the molecular events occurring during infection of a host that are inaccessible using vertebrate hosts. RESULTS: To profile the transcriptional response of an infective nematode during the early stage of infection, we performed next generation RNA sequencing on H. bacteriophora IJs incubated in Manduca sexta hemolymph plasma for 9 h. A subset of up-regulated and down-regulated genes were validated using qRT-PCR. Comparative analysis of the transcriptome with untreated controls found a number of differentially expressed genes (DEGs) which cover a number of different functional categories. A subset of DEGs is conserved across Clade V parasitic nematodes revealing an array of candidate parasitic genes. CONCLUSIONS: Our analysis reveals transcriptional changes in the regulation of a large number of genes, most of which have not been shown previously to play a role in the process of infection. A significant proportion of these genes are unique to parasitic nematodes, suggesting the identification of a group of parasitism factors within nematodes. Future studies using these candidates may provide functional insight into the process of nematode parasitism and also the molecular evolution of parasitism within nematodes.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica
Genes de Helmintos
Rhabditoidea/genética
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Biologia Computacional/métodos
Ontologia Genética
Sequenciamento de Nucleotídeos em Larga Escala
Interações Hospedeiro-Patógeno
Anotação de Sequência Molecular
Reprodutibilidade dos Testes
Infecções por Rhabditida/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170105
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-016-3468-6


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[PMID]:27555343
[Au] Autor:Tobias NJ; Heinrich AK; Eresmann H; Wright PR; Neubacher N; Backofen R; Bode HB
[Ad] Endereço:Fachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe Universität Frankfurt, Frankfurt am Main, Germany.
[Ti] Título:Photorhabdus-nematode symbiosis is dependent on hfq-mediated regulation of secondary metabolites.
[So] Source:Environ Microbiol;19(1):119-129, 2017 Jan.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Photorhabdus luminescens maintains a symbiotic relationship with the nematodes Heterorhabditis bacteriophora and together they infect and kill insect larvae. To maintain this symbiotic relationship, the bacteria must produce an array of secondary metabolites to assist in the development and replication of nematodes. The regulatory mechanisms surrounding production of these compounds are mostly unknown. The global post-transcriptional regulator, Hfq, is widespread in bacteria and performs many functions, one of which is the facilitation of sRNA binding to target mRNAs, with recent research thoroughly exploring its various pleiotropic effects. Here we generate and characterize an hfq deletion mutant and show that in the absence of hfq, the bacteria are no longer able to maintain a healthy symbiosis with nematodes due to the abolishment of the production of all known secondary metabolites. RNAseq led us to produce a second deletion of a known repressor, HexA, in the same strain, which restored both metabolite production and symbiosis.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Proteínas de Ligação a DNA/genética
Fator Proteico 1 do Hospedeiro/genética
Photorhabdus/genética
Rhabditoidea/microbiologia
Metabolismo Secundário/genética
[Mh] Termos MeSH secundário: Animais
Insetos/microbiologia
Insetos/parasitologia
Photorhabdus/fisiologia
RNA Mensageiro/genética
Pequeno RNA não Traduzido/genética
Simbiose/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA-Binding Proteins); 0 (HexA protein, bacteria); 0 (Host Factor 1 Protein); 0 (RNA, Messenger); 0 (RNA, Small Untranslated)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160825
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13502


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[PMID]:27543560
[Au] Autor:Maher AM; Asaiyah MA; Brophy C; Griffin CT
[Ad] Endereço:Department of Biology, Maynooth University, Maynooth, County Kildare, Ireland.
[Ti] Título:An Entomopathogenic Nematode Extends Its Niche by Associating with Different Symbionts.
[So] Source:Microb Ecol;73(1):211-223, 2017 Jan.
[Is] ISSN:1432-184X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial symbionts are increasingly recognised as mediators of ecologically important traits of their animal hosts, with acquisition of new traits possible by uptake of novel symbionts. The entomopathogenic nematode Heterorhabditis downesi associates with two bacterial symbionts, Photorhabdus temperata subsp. temperata and P. temperata subsp. cinerea. At one intensively studied coastal dune site, P. temperata subsp. cinerea is consistently more frequently isolated than P. temperata subsp. temperata in H. downesi recovered from under the bare sand/Ammophila arrenaria of the front dunes (where harsh conditions, including drought, prevail). This is not the case in the more permissive closed dune grassland further from the sea. No differences were detected in ITS1 (internal transcribed spacer) sequence between nematode lines carrying either of the two symbiont subspecies, nor did they differ in their ability to utilise insects from three orders. The two symbionts could be readily swapped between lines, and both were carried in equal numbers within infective juveniles. In laboratory experiments, we tested whether the symbionts differentially affected nematode survival in insect cadavers that were allowed to dry. We assessed numbers of nematode infective juveniles emerging from insects that had been infected with H. downesi carrying either symbiont subspecies and then allowed to desiccate for up to 62 days. In moist conditions, cadavers produced similar numbers of nematodes, irrespective of the symbiont subspecies present, while under desiccating conditions, P. temperata subsp. cinerea cadavers yielded more nematode progeny than P. temperata subsp. temperata cadavers. Desiccating cadavers with the same nematode isolates, carrying either one or the other symbiont subspecies, confirmed that the symbiont was responsible for differences in nematode survival. Moreover, cadavers harbouring P. temperata subsp. cinerea had a reduced rate of drying relative to cadavers harbouring P. temperata subsp. temperata. Our experiments support the hypothesis that H. downesi can extend its niche into harsher conditions by associating with P. temperata subsp. cinerea.
[Mh] Termos MeSH primário: Interações Hospedeiro-Patógeno/fisiologia
Photorhabdus/isolamento & purificação
Photorhabdus/fisiologia
Rhabditoidea/microbiologia
[Mh] Termos MeSH secundário: Animais
DNA Intergênico/genética
Dessecação
Irlanda
Photorhabdus/genética
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Intergenic)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160821
[St] Status:MEDLINE
[do] DOI:10.1007/s00248-016-0829-2


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[PMID]:27346628
[Au] Autor:Morais DH; Aguiar A; Müller MI; Narciso RB; da Silva LA; da Silva RJ
[Ad] Endereço:UNESP - Univ. Estadual Paulista,Campus de Botucatu,Instituto de Biociências,Departamento de Parasitologia,Av. Bento Lopes s/n Distrito de Rubião Junior CEP 18080-970,Botucatu,SP,Brazil.
[Ti] Título:Morphometric and phylogenetic analyses of Serpentirhabdias viperidicus n. sp. (Nematoda: Rhabdiasidae) from the lancehead snake Bothrops moojeni Hoge, 1966 (Reptilia: Serpentes: Viperidae) in Brazil.
[So] Source:J Helminthol;91(3):360-370, 2017 May.
[Is] ISSN:1475-2697
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Serpentirhabdias viperidicus n. sp. (Nematoda: Rhabdiasidae) is described from the lungs of the 'Brazilian lancehead' Bothrops moojeni (Hoge, 1966) from the savannah in São Paulo State, Brazil. The new species is the eighth species of Serpentirhabdias described in the Neotropical region, and differs from other species mainly by a combination of characters: lips slightly notable, presence of fine striations at posterior ends, presence of two parallel lines with intercalated pores, a pore-shaped phasmid situated at the level of the anal aperture and another two in the posterior half of the tail. It is the first species of Serpentirhabdias reported in this snake host and the second species of this genus found parasitizing South American viperidian snakes. Molecular phylogenetic analysis using ribosomal (ITS and 28S partial) genes confirms Serpentirhabdias viperidicus n. sp. as a new species that clustered in the Serpentirhabdias clade, sister taxon to Serpentirhabdias fuscovenosa and Serpentirhabdias elaphe. This is the first description of Serpentirhabdias species from Brazil using molecular approaches and morphological characters to confirm the monophyly of this recent genus.
[Mh] Termos MeSH primário: Bothrops/parasitologia
Infecções por Rhabditida/veterinária
Rhabditoidea/classificação
Rhabditoidea/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Brasil
Análise por Conglomerados
DNA de Helmintos/química
DNA de Helmintos/genética
DNA Ribossômico/química
DNA Ribossômico/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Pulmão/parasitologia
Microscopia
Filogenia
RNA Ribossômico 28S/genética
Infecções por Rhabditida/parasitologia
Rhabditoidea/anatomia & histologia
Rhabditoidea/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth); 0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 28S)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160628
[St] Status:MEDLINE
[do] DOI:10.1017/S0022149X16000377


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[PMID]:27052466
[Au] Autor:Shahina F; Tabassum KA; Salma J; Mehreen G; Knoetze R
[Ad] Endereço:National Nematological Research Centre,University of Karachi,Karachi-75270,Pakistan.
[Ti] Título:Heterorhabditis pakistanense n. sp. (Nematoda: Heterorhabditidae) a new entomopathogenic nematode from Pakistan.
[So] Source:J Helminthol;91(2):222-235, 2017 Mar.
[Is] ISSN:1475-2697
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new entomopathogenic nematode species of Heterorhabditis, described as H. pakistanense n. sp., was isolated from soil samples around the roots of grass at Malir, Karachi, Sindh, Pakistan. The new species is characterized morphologically by features of males: body size 819 µm (720-1013 µm), D% ((distance from anterior end to excretory pore divided by pharynx length) × 100) 119 (110-126), SW% ((spicule length divided by anal body diameter) × 100) 156 (144-191), GS% ((gubernaculum length divided by spicule length) × 100) 58 (48-65) and variations in the number of bursal papillae of the terminal group: 8th and 9th papillae sometimes absent on both sides, sometimes eight papillae present on the right side whereas six papillae present on the left side. On the right side the arrangement of papillae is 1 + 2 + 3 + 2 whereas on the left side it is 1 + 2 + 3. The hermaphrodite has a prominent post-anal swelling and a conoid tail 82 µm (64-95 µm) long with a pointed terminus. Hermaphrodites of H. pakistanense n. sp. can be distinguished from all species of Heterorhabditis except H. downesi by having a mucronate tail. Infective juveniles have a medium-sized body (581 µm (558-624 µm)), long pharynx (117 µm (113-125 µm)), ensheathed tail (99 µm (95-110 µm)) and E% ((distance from anterior end to excretory pore divided by tail length) × 100) 100 (95-107). The new species can be distinguished from all species of Heterorhabditis by the absence of the 7th, 8th and 9th bursal papillae. Heterorhabditis pakistanense is further characterized by the internal transcribed spacer (ITS) and the D2D3 region of the 28S rDNA gene. The closest species H. indica, H. gerrardi, H. amazonensis and H. noenieputensis being separated by 9, 7, 66 and 15 bp, respectively, in the ITS region. Molecular phylogenetic trees based on sequences of ITS rDNA, D2D3 regions and the mitochondrial 12S rRNA gene support the description of H. pakistanense as a new species.
[Mh] Termos MeSH primário: Insetos/parasitologia
Rhabditoidea/isolamento & purificação
Solo/parasitologia
[Mh] Termos MeSH secundário: Animais
Tamanho Corporal
Feminino
Insetos/classificação
Masculino
Paquistão
Filogenia
Rhabditoidea/classificação
Rhabditoidea/genética
Rhabditoidea/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160408
[St] Status:MEDLINE
[do] DOI:10.1017/S0022149X16000158



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