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[PMID]:28427478
[Au] Autor:Uni S; Mat Udin AS; Agatsuma T; Saijuntha W; Junker K; Ramli R; Omar H; Lim YA; Sivanandam S; Lefoulon E; Martin C; Belabut DM; Kasim S; Abdullah Halim MR; Zainuri NA; Bhassu S; Fukuda M; Matsubayashi M; Harada M; Low VL; Chen CD; Suganuma N; Hashim R; Takaoka H; Azirun MS
[Ad] Endereço:Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia. unishigehiko@um.edu.my.
[Ti] Título:Morphological and molecular characteristics of Malayfilaria sofiani Uni, Mat Udin & Takaoka n. g., n. sp. (Nematoda: Filarioidea) from the common treeshrew Tupaia glis Diard & Duvaucel (Mammalia: Scandentia) in Peninsular Malaysia.
[So] Source:Parasit Vectors;10(1):194, 2017 Apr 20.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The filarial nematodes Wuchereria bancrofti (Cobbold, 1877), Brugia malayi (Brug, 1927) and B. timori Partono, Purnomo, Dennis, Atmosoedjono, Oemijati & Cross, 1977 cause lymphatic diseases in humans in the tropics, while B. pahangi (Buckley & Edeson, 1956) infects carnivores and causes zoonotic diseases in humans in Malaysia. Wuchereria bancrofti, W. kalimantani Palmieri, Pulnomo, Dennis & Marwoto, 1980 and six out of ten Brugia spp. have been described from Australia, Southeast Asia, Sri Lanka and India. However, the origin and evolution of the species in the Wuchereria-Brugia clade remain unclear. While investigating the diversity of filarial parasites in Malaysia, we discovered an undescribed species in the common treeshrew Tupaia glis Diard & Duvaucel (Mammalia: Scandentia). METHODS: We examined 81 common treeshrews from 14 areas in nine states and the Federal Territory of Peninsular Malaysia for filarial parasites. Once any filariae that were found had been isolated, we examined their morphological characteristics and determined the partial sequences of their mitochondrial cytochrome c oxidase subunit 1 (cox1) and 12S rRNA genes. Polymerase chain reaction (PCR) products of the internal transcribed spacer 1 (ITS1) region were then cloned into the pGEM-T vector, and the recombinant plasmids were used as templates for sequencing. RESULTS: Malayfilaria sofiani Uni, Mat Udin & Takaoka, n. g., n. sp. is described based on the morphological characteristics of adults and microfilariae found in common treeshrews from Jeram Pasu, Kelantan, Malaysia. The Kimura 2-parameter distance between the cox1 gene sequences of the new species and W. bancrofti was 11.8%. Based on the three gene sequences, the new species forms a monophyletic clade with W. bancrofti and Brugia spp. The adult parasites were found in tissues surrounding the lymph nodes of the neck of common treeshrews. CONCLUSIONS: The newly described species appears most closely related to Wuchereria spp. and Brugia spp., but differs from these in several morphological characteristics. Molecular analyses based on the cox1 and 12S rRNA genes and the ITS1 region indicated that this species differs from both W. bancrofti and Brugia spp. at the genus level. We thus propose a new genus, Malayfilaria, along with the new species M. sofiani.
[Mh] Termos MeSH primário: Filariose/veterinária
Filarioidea/anatomia & histologia
Filarioidea/genética
Tupaia/parasitologia
[Mh] Termos MeSH secundário: Animais
Brugia/anatomia & histologia
Brugia/genética
DNA Espaçador Ribossômico/genética
Feminino
Filariose/epidemiologia
Filariose/parasitologia
Filarioidea/isolamento & purificação
Malásia
Masculino
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Wuchereria/anatomia & histologia
Wuchereria/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2105-9


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[PMID]:27756458
[Au] Autor:Kastner RJ; Stone CM; Steinmann P; Tanner M; Tediosi F
[Ad] Endereço:Swiss Tropical and Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland.
[Ti] Título:Lessons Learned From Developing an Eradication Investment Case for Lymphatic Filariasis.
[So] Source:Adv Parasitol;94:393-417, 2016.
[Is] ISSN:2163-6079
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the last few years, the concepts of disease elimination and eradication have again gained consideration from the global health community, with Guinea worm disease (dracunculiasis) on track to become the first parasitic disease to be eradicated. Given the many complex and interlinking issues involved in committing to a disease eradication initiative, such commitments must be based on a solid assessment of a broad range of factors. In this chapter, we discuss the value and implications of undertaking a systematic and fact-based analysis of the overall situation prior to embarking on an elimination or eradication programme. As an example, we draw upon insights gained from a series of lymphatic filariasis (LF) studies from our research group that adopted an eradication investment case (EIC) framework. The justification for EICs, and related epidemiological, geospatial and other mathematical/operational research modelling, stems from the necessity for proper planning prior to committing to disease eradication. Across all considerations for LF eradication, including: time, treatments, level of investments necessary, health impact, cost-effectiveness, and broader economic benefits, scaling-up mass drug administration coverage to all endemic communities immediately provided the most favourable results. The coherent and consistent pursuit of eradication goals, operationally tailored to a given socioecological system and based on integrated measures of available tools will lead relatively rapidly to elimination in many parts of endemic areas and provide the cornerstone towards eradication.
[Mh] Termos MeSH primário: Anti-Helmínticos/administração & dosagem
Brugia/efeitos dos fármacos
Filariose Linfática/prevenção & controle
Modelos Teóricos
[Mh] Termos MeSH secundário: Animais
Análise Custo-Benefício
Erradicação de Doenças
Filariose Linfática/tratamento farmacológico
Filariose Linfática/economia
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Anthelmintics)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170310
[Lr] Data última revisão:
170310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE


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[PMID]:27097561
[Au] Autor:Suh A; Witt CC; Menger J; Sadanandan KR; Podsiadlowski L; Gerth M; Weigert A; McGuire JA; Mudge J; Edwards SV; Rheindt FE
[Ad] Endereço:Department of Evolutionary Biology, Evolutionary Biology Centre (EBC), Uppsala University, SE-752 36 Uppsala, Sweden.
[Ti] Título:Ancient horizontal transfers of retrotransposons between birds and ancestors of human pathogenic nematodes.
[So] Source:Nat Commun;7:11396, 2016 Apr 21.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Parasite host switches may trigger disease emergence, but prehistoric host ranges are often unknowable. Lymphatic filariasis and loiasis are major human diseases caused by the insect-borne filarial nematodes Brugia, Wuchereria and Loa. Here we show that the genomes of these nematodes and seven tropical bird lineages exclusively share a novel retrotransposon, AviRTE, resulting from horizontal transfer (HT). AviRTE subfamilies exhibit 83-99% nucleotide identity between genomes, and their phylogenetic distribution, paleobiogeography and invasion times suggest that HTs involved filarial nematodes. The HTs between bird and nematode genomes took place in two pantropical waves, >25-22 million years ago (Myr ago) involving the Brugia/Wuchereria lineage and >20-17 Myr ago involving the Loa lineage. Contrary to the expectation from the mammal-dominated host range of filarial nematodes, we hypothesize that these major human pathogens may have independently evolved from bird endoparasites that formerly infected the global breadth of avian biodiversity.
[Mh] Termos MeSH primário: Doenças das Aves/história
Brugia/genética
Filariose Linfática/história
Filariose/história
Transferência Genética Horizontal
Loa/genética
Loíase/história
Wuchereria/genética
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Doenças das Aves/epidemiologia
Doenças das Aves/parasitologia
Doenças das Aves/transmissão
Aves/classificação
Aves/parasitologia
Brugia/classificação
Filariose Linfática/epidemiologia
Filariose Linfática/parasitologia
Filariose Linfática/transmissão
Filariose/epidemiologia
Filariose/parasitologia
Filariose/transmissão
História Antiga
Seres Humanos
Loa/classificação
Loíase/epidemiologia
Loíase/parasitologia
Loíase/transmissão
Filogenia
Filogeografia
Retroelementos
Wuchereria/classificação
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Retroelements)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160422
[St] Status:MEDLINE
[do] DOI:10.1038/ncomms11396


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[PMID]:27008279
[Au] Autor:Sanpool O; Tantrawatpan C; Thanchomnang T; Janwan P; Intapan PM; Rodpai R; Lulitanond V; Taweethavonsawat P; Maleewong W
[Ad] Endereço:1 Department of Parasitology, Faculty of Medicine, Khon Kaen University , Khon Kaen, Thailand .
[Ti] Título:Pyrosequencing Using SL and 5S rRNA as Molecular Markers for Identifying Zoonotic Filarial Nematodes in Blood Samples and Mosquitoes.
[So] Source:Vector Borne Zoonotic Dis;16(5):326-33, 2016 May.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUNDS: Lymphatic filariasis is principally caused by Wuchereria bancrofti, and Brugia malayi. The other two filarial nematode species, Brugia pahangi and Dirofilaria immitis, possibly cause human zoonotic diseases. METHODS: We propose the development of a PCR assay linked with DNA pyrosequencing as a rapid tool to identify W. bancrofti, B. malayi, B. pahangi, and D. immitis in blood samples and mosquitoes. Primers targeting the fragment of the 5S ribosomal RNA and spliced leader sequences were newly designed and developed to identify these four filarial nematodes. Analytical sensitivity and specificity were evaluated. RESULTS: Pyrosequencing determination of nucleotide variations within 36 nucleotides for B. malayi and B. pahangi, and 32 nucleotides for W. bancrofti and D. immitis is sufficient for differentiation of those filarial nematodes, and for detection of intraspecies genetic variation of B. malayi. This analysis could detect a single B. malayi, B. pahangi, W. bancrofti, and D. immitis microfilaria in blood samples. CONCLUSIONS: Overall, the PCR-linked pyrosequencing-based method was faster than direct sequencing and less expensive than real-time PCR or direct sequencing. This is the possibility of choice that can be applied in a high-throughput platform for identification and surveillance of reservoirs and vectors infected with lymphatic filaria in endemic areas.
[Mh] Termos MeSH primário: Brugia/isolamento & purificação
Dirofilaria immitis/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
RNA de Helmintos/isolamento & purificação
Wuchereria bancrofti/isolamento & purificação
Zoonoses
[Mh] Termos MeSH secundário: Aedes/parasitologia
Animais
Biomarcadores
Doenças do Gato/parasitologia
Gatos
Doenças do Cão/parasitologia
Cães
Seres Humanos
RNA de Protozoário/genética
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (RNA, Helminth); 0 (RNA, Protozoan)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160324
[St] Status:MEDLINE
[do] DOI:10.1089/vbz.2015.1914


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[PMID]:26427536
[Au] Autor:Dewi RM; Tuti S; Ganefa S; Anwar C; Larasati R; Ariyanti E; Herjati H; Brady M
[Ad] Endereço:National Institute of Health Research and Development, Indonesia Ministry of Health, Jl. Percetakan Negara No. 29, Jakarta, 10560, Indonesia. rmdewi@gmail.com.
[Ti] Título:Brugia Rapid™ antibody responses in communities of Indonesia in relation to the results of 'transmission assessment surveys' (TAS) for the lymphatic filariasis elimination program.
[So] Source:Parasit Vectors;8:499, 2015 Oct 01.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The Global Programme to Eliminate Lymphatic Filariasis recommends the transmission assessment survey (TAS) as the preferred methodology for determining whether mass drug administration can be stopped in an endemic area. Because of the limited experience available globally with the use of Brugia Rapid™ tests in conducting TAS in Brugia spp. areas, we explored the relationship between the antibody test results and Brugia spp. infection as detected by microfilaremia in different epidemiological settings. METHODS: The study analyzes the Brugia Rapid™ antibody responses and microfilaremia in all ages at three study sites in: i) a district which was classified as non-endemic, ii) a district which passed TAS, and iii) a district which failed TAS. Convenience sampling was done in each site, in one to three purposefully selected villages with a goal of 500 samples in each district. RESULTS: A total of 1543 samples were collected from residents in all three study sites. In the site which was classified as non-endemic and where MDA had not been conducted, 5 % of study participants were antibody positive, none was positive for microfilaremia, and age-specific antibody prevalence peaked at almost 8 % in the 25-34 year-old age range, with no antibody-positive results found in children under eight years of age. In the site that had passed TAS, 1 % of participants were antibody positive and none was positive for microfilaremia. In the site which failed TAS, 15 % of participants were antibody positive, 0.2 % were microfilaremic, and age-specific antibody prevalence was highest in 6-7 year olds (30 %), but above 8 % in all age levels above 8 years old. CONCLUSIONS: These results from districts which followed the current WHO guidance for mapping, MDA, and implementing TAS, while providing antibody profiles of treated and untreated populations under programmatic settings, support the choice of antibody prevalence in the 6- and 7-year-old age group in TAS for making stopping MDA decisions. Since only one study participant was microfilaremic, no conclusions could be drawn about the relationship between antibodies and microfilaremia and further longitudinal studies are required to understand this relationship.
[Mh] Termos MeSH primário: Anticorpos Anti-Helmínticos/sangue
Brugia/imunologia
Filariose Linfática/diagnóstico
Filariose Linfática/prevenção & controle
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Criança
Pré-Escolar
Transmissão de Doença Infecciosa/prevenção & controle
Filariose Linfática/epidemiologia
Filariose Linfática/imunologia
Monitoramento Epidemiológico
Feminino
Filaricidas/uso terapêutico
Saúde Global
Seres Humanos
Indonésia/epidemiologia
Masculino
Meia-Idade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Filaricides)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151003
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-015-1093-x


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[PMID]:25695776
[Au] Autor:Nutting CS; Eversole RR; Blair K; Specht S; Nutman TB; Klion AD; Wanji S; Boussinesq M; Mackenzie CD
[Ad] Endereço:Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan, United States of America.
[Ti] Título:Analysis of nematode motion using an improved light-scatter based system.
[So] Source:PLoS Negl Trop Dis;9(2):e0003523, 2015 Feb.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The detailed assessment of nematode activity and viability still remains a relatively undeveloped area of biological and medical research. Computer-based approaches to assessing the motility of larger nematode stages have been developed, yet these lack the capability to detect and analyze the more subtle and important characteristics of the motion of nematodes. There is currently a need to improved methods of assessing the viability and health of parasitic worms. METHODS: We describe here a system that converts the motion of nematodes through a light-scattering system into an electrical waveform, and allows for reproducible, and wholly non-subjective, assessment of alterations in motion, as well as estimation of the number of nematode worms of different forms and sizes. Here we have used Brugia sp. microfilariae (L1), infective larvae (L3) and adults, together with the free-living nematode Caenorhabditis elegans. RESULTS: The motion of worms in a small (200 ul) volume can be detected, with the presence of immotile worms not interfering with the readings at practical levels (up to at least 500 L1 /200 ul). Alterations in the frequency of parasite movement following the application of the anti-parasitic drugs, (chloroquine and imatinib); the anti-filarial effect of the latter agent is the first demonstrated here for the first time. This system can also be used to estimate the number of parasites, and shortens the time required to estimate parasites numbers, and eliminates the need for microscopes and trained technicians to provide an estimate of microfilarial sample sizes up to 1000 parasites/ml. Alterations in the form of motion of the worms can also be depicted. CONCLUSIONS: This new instrument, named a "WiggleTron", offers exciting opportunities to further study nematode biology and to aid drug discovery, as well as contributing to a rapid estimate of parasite numbers in various biological samples.
[Mh] Termos MeSH primário: Brugia/fisiologia
Caenorhabditis elegans/fisiologia
Locomoção/fisiologia
[Mh] Termos MeSH secundário: Animais
Antiprotozoários/farmacologia
Brugia/efeitos dos fármacos
Caenorhabditis elegans/efeitos dos fármacos
Cloroquina/farmacologia
Descoberta de Drogas
Feminino
Mesilato de Imatinib/farmacologia
Larva/efeitos dos fármacos
Locomoção/efeitos dos fármacos
Microfilárias/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 886U3H6UFF (Chloroquine); 8A1O1M485B (Imatinib Mesylate)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150220
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0003523


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[PMID]:25851428
[Au] Autor:Paniz Mondolfi AE; Sordillo EM
[Ad] Endereço:Department of Pathology and Laboratory Medicine and the Division of Infectious Diseases, Barquisimeto, Edo. Lara, Venezuela. alberto.paniz-mondolfi@yale.edu.
[Ti] Título:Invited editorial: zoonotic lymphatic filariasis in the Americas: trends in epidemiology, diagnosis and treatment, with special emphasis on brugian filariasis.
[So] Source:Recent Pat Antiinfect Drug Discov;9(3):161-3, 2014.
[Is] ISSN:2212-4071
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Mh] Termos MeSH primário: Brugia/patogenicidade
Doenças Transmissíveis Emergentes/parasitologia
Filariose Linfática/parasitologia
Zoonoses/parasitologia
[Mh] Termos MeSH secundário: Américas/epidemiologia
Animais
Doenças Transmissíveis Emergentes/diagnóstico
Doenças Transmissíveis Emergentes/epidemiologia
Doenças Transmissíveis Emergentes/prevenção & controle
Doenças Transmissíveis Emergentes/transmissão
Vetores de Doenças
Filariose Linfática/diagnóstico
Filariose Linfática/epidemiologia
Filariose Linfática/prevenção & controle
Filariose Linfática/transmissão
Filaricidas/uso terapêutico
Seres Humanos
Valor Preditivo dos Testes
Fatores de Risco
Resultado do Tratamento
Zoonoses/diagnóstico
Zoonoses/epidemiologia
Zoonoses/prevenção & controle
Zoonoses/transmissão
[Pt] Tipo de publicação:EDITORIAL
[Nm] Nome de substância:
0 (Filaricides)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150520
[Lr] Data última revisão:
150520
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150409
[St] Status:MEDLINE


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[PMID]:24963722
[Au] Autor:Paniz-Mondolfi AE; Gárate T; Stavropoulos C; Fan W; González LM; Eberhard M; Kimmelstiel F; Sordillo EM
[Ti] Título:Zoonotic filariasis caused by novel Brugia sp. nematode, United States, 2011.
[So] Source:Emerg Infect Dis;20(7):1248-50, 2014 Jul.
[Is] ISSN:1080-6059
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Brugia/isolamento & purificação
Filariose/diagnóstico
Filariose/microbiologia
Zoonoses/diagnóstico
Zoonoses/microbiologia
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Masculino
Meia-Idade
Estados Unidos
[Pt] Tipo de publicação:CASE REPORTS; LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1503
[Cu] Atualização por classe:150805
[Lr] Data última revisão:
150805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140626
[St] Status:MEDLINE
[do] DOI:10.3201/eid2007.131654


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[PMID]:24827444
[Au] Autor:Breaux JA; Schumacher MK; Juliano SA
[Ad] Endereço:School of Biological Sciences, Illinois State University, Normal, IL 61790-4120, USA jabreau@ilstu.edu.
[Ti] Título:What does not kill them makes them stronger: larval environment and infectious dose alter mosquito potential to transmit filarial worms.
[So] Source:Proc Biol Sci;281(1786), 2014 Jul 07.
[Is] ISSN:1471-2954
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:For organisms with complex life cycles, larval environments can modify adult phenotypes. For mosquitoes and other vectors, when physiological impacts of stressors acting on larvae carry over into the adult stage they may interact with infectious dose of a vector-borne pathogen, producing a range of phenotypes for vector potential. Investigation of impacts of a common source of stress, larval crowding and intraspecific competition, on adult vector interactions with pathogens may increase our understanding of the dynamics of pathogen transmission by mosquito vectors. Using Aedes aegypti and the nematode parasite Brugia pahangi, we demonstrate dose dependency of fitness effects of B. pahangi infection on the mosquito, as well as interactions between competitive stress among larvae and infectious dose for resulting adults that affect the physiological and functional ability of mosquitoes to act as vectors. Contrary to results from studies on mosquito-arbovirus interactions, our results suggest that adults from crowded larvae may limit infection better than do adults from uncrowded controls, and that mosquitoes from high-quality larval environments are more physiologically and functionally capable vectors of B. pahangi. Our results provide another example of how the larval environment can have profound effects on vector potential of resulting adults.
[Mh] Termos MeSH primário: Aedes/fisiologia
Aedes/parasitologia
Brugia/fisiologia
Insetos Vetores/fisiologia
Insetos Vetores/parasitologia
[Mh] Termos MeSH secundário: Aedes/genética
Aedes/crescimento & desenvolvimento
Animais
Fertilidade
Aptidão Genética
Insetos Vetores/genética
Insetos Vetores/crescimento & desenvolvimento
Larva/crescimento & desenvolvimento
Larva/fisiologia
Longevidade
Densidade Demográfica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1501
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140516
[St] Status:MEDLINE


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[PMID]:24479705
[Au] Autor:Ali M; Afzal M; Abdul Nasim S; Ahmad I
[Ad] Endereço:Nanomedicine Lab, Hamdard Nanobiotechnology Center for Advanced Research, Faculty of Engineering & Interdisciplinary Sciences , Hamdard University, New Delhi , India .
[Ti] Título:Nanocurcumin: a novel antifilarial agent with DNA topoisomerase II inhibitory activity.
[So] Source:J Drug Target;22(5):395-407, 2014 Jun.
[Is] ISSN:1029-2330
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The aim of this study is to evaluate the antifilarial, antiwolbachial and DNA topoisomerase II inhibitory activity of nanocurcumin (nano-CUR). METHODS: Nano-CUR formulations (F1-F6) were prepared using free radical polymerization and were characterized by particle size, morphology, encapsulation efficiency and in vitro release kinetics. Antifilarial potential was evaluated in vivo against Brugian filariasis in an experimental rodent model, Mastomys coucha, by selecting the formulation that maximized parasite elimination characteristics. Wolbachial status was determined by PCR and a relaxation assay was used to estimate DNA topoisomerase II inhibitory activity. RESULTS: Nano-CUR (F3) having a 60 nm diameter and 89.78% entrapment efficiency showed the most favorable characteristics for the elimination of filarial parasites. In vivo pharmacokinetic and organ distribution studies demonstrate significantly greater C(max) (86.6 ± 2.56 ng ml(-1)), AUC0-∞ (796 ± 89.8 ng d ml(-1)), MRT (19.5 ± 7.82 days) and bioavailability of CUR (70.02%) in the organs from which the adult parasites were recovered. The optimized nano-CUR (F3) (5 × 5 mg/kg, orally) significantly augmented the microfilariciadal and adulticidal action of CUR over free CUR (5 × 50 mg/kg, orally) or Diethylcarbamizine (50 mg/kg, orally) against the Brugia malayi Mastomys coucha rodent model. The PCR results showed complete elimination of wolbachia from the recovered female parasites. Interestingly, nano-CUR was also found to be a novel inhibitor of filarial worm DNA topoisomerase II, Setaria Cervi in vitro. CONCLUSION: This study recognizes the beforehand antimicrofilarial, antimacrofilarial, anti-wolbachial activity of nano-CUR (F3) over free forms and additionally its strong inhibitory action against the major target filarial parasite enzyme DNA topoisomerase II in vitro.
[Mh] Termos MeSH primário: Curcumina/uso terapêutico
Portadores de Fármacos/química
Filariose Linfática/tratamento farmacológico
Filaricidas/uso terapêutico
Nanopartículas/química
Inibidores da Topoisomerase II/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Brugia/efeitos dos fármacos
Brugia/enzimologia
Brugia/fisiologia
Curcumina/administração & dosagem
Curcumina/farmacocinética
Modelos Animais de Doenças
Liberação Controlada de Fármacos
Filariose Linfática/parasitologia
Filaricidas/administração & dosagem
Interações Hospedeiro-Parasita/fisiologia
Masculino
Camundongos
Tamanho da Partícula
Ratos
Propriedades de Superfície
Distribuição Tecidual
Inibidores da Topoisomerase II/administração & dosagem
Inibidores da Topoisomerase II/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drug Carriers); 0 (Filaricides); 0 (Topoisomerase II Inhibitors); IT942ZTH98 (Curcumin)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:140514
[Lr] Data última revisão:
140514
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140201
[St] Status:MEDLINE
[do] DOI:10.3109/1061186X.2013.869823



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