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[PMID]:28453850
[Au] Autor:Mukherjee S; Mukherjee S; Maiti TK; Bhattacharya S; Sinha Babu SP
[Ad] Endereço:Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, West Bengal, India.
[Ti] Título:A Novel Ligand of Toll-like Receptor 4 From the Sheath of Wuchereria bancrofti Microfilaria Induces Proinflammatory Response in Macrophages.
[So] Source:J Infect Dis;215(6):954-965, 2017 03 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: Lymphatic filariasis, frequently caused from Wuchereria bancrofti infection, is endemic in several parts of the globe and responsible for human health problems and socioeconomic loss to a large extent. Inflammatory consequences originating from host-parasite interaction play a major role in the disease pathology and allied complications. The identity of the key mediator of this process is yet unknown in filarial research. Methods: Microfilarial protein (MfP) was isolated from the sheath of W. bancrofti microfilariae through ultrafiltration, followed by chromatographic separation. Expression of signaling molecules was studied by enzyme-linked immunosorbent assay and immunoblotting. Binding of MfP to Toll-like receptor 4 (TLR4) was determined by co-immunoprecipitation, fluorescein isothiocyanate-probing, and surface plasmon resonance analysis. Results: We found that MfP (approximately 70 kDa) binds to macrophage-TLR4 and triggers nuclear factor kappa beta activation that upregulates secretion of proinflammatory cytokines. Microfilarial protein failed to induce inflammation in either TLRKO macrophage or macrophage treated with TLR4 inhibitor, indicating that MfP acts through TLR4. We have also detected phenotypic transformation of macrophages from anti-inflammatory (M2) to proinflammatory (M1) subtype after incubation with MfP. Conclusions: Microfilarial protein appears to be a new ligand of TLR4 from W. bancrofti. Determination of its functional attributions in the host-parasite relationship, especially immunopathogenesis of filarial infection, may improve our understanding.
[Mh] Termos MeSH primário: Antígenos de Helmintos/imunologia
Proteínas de Helminto/imunologia
Macrófagos/imunologia
Receptor 4 Toll-Like/imunologia
Wuchereria bancrofti/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Anti-Helmínticos/sangue
Células Cultivadas
Ensaio de Imunoadsorção Enzimática
Células HEK293
Interações Hospedeiro-Parasita
Seres Humanos
Ligantes
Camundongos
Camundongos Endogâmicos BALB C
Microfilárias/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Helminth Proteins); 0 (Ligands); 0 (Toll-Like Receptor 4)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix067


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[PMID]:29028167
[Ti] Título:Progress towards eliminating onchocerciasis in the WHO Region of the Americas: elimination of transmission in the north-east focus of the Bolivarian Republic of Venezuela.
[Ti] Título:Progrès vers l'élimination de l'onchocercose dans la Région OMS des Amériques: élimination de la transmission dans le foyer du nord-est de la République bolivarienne du Venezuela..
[So] Source:Wkly Epidemiol Rec;92(41):617-23, 2017 10 13.
[Is] ISSN:0049-8114
[Cp] País de publicação:Switzerland
[La] Idioma:eng; fre
[Mh] Termos MeSH primário: Erradicação de Doenças/tendências
Onchocerca volvulus
Oncocercose/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Antiparasitários/uso terapêutico
Erradicação de Doenças/métodos
Vetores de Doenças
Seres Humanos
Índios Sul-Americanos
Ivermectina/uso terapêutico
Microfilárias/efeitos dos fármacos
Onchocerca volvulus/efeitos dos fármacos
Oncocercose/transmissão
Avaliação de Programas e Projetos de Saúde
Fatores de Tempo
Venezuela
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiparasitic Agents); 70288-86-7 (Ivermectin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171015
[St] Status:MEDLINE


  3 / 2245 MEDLINE  
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[PMID]:28892473
[Au] Autor:Chesnais CB; Awaca-Uvon NP; Bolay FK; Boussinesq M; Fischer PU; Gankpala L; Meite A; Missamou F; Pion SD; Weil GJ
[Ad] Endereço:IRD UMI 233-INSERM U1175-Montpellier University, Montpellier, France.
[Ti] Título:A multi-center field study of two point-of-care tests for circulating Wuchereria bancrofti antigenemia in Africa.
[So] Source:PLoS Negl Trop Dis;11(9):e0005703, 2017 Sep.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The Global Programme to Eliminate Lymphatic Filariasis uses point-of-care tests for circulating filarial antigenemia (CFA) to map endemic areas and for monitoring and evaluating the success of mass drug administration (MDA) programs. We compared the performance of the reference BinaxNOW Filariasis card test (ICT, introduced in 1997) with the Alere Filariasis Test Strip (FTS, introduced in 2013) in 5 endemic study sites in Africa. METHODOLOGY: The tests were compared prior to MDA in two study sites (Congo and Côte d'Ivoire) and in three sites that had received MDA (DRC and 2 sites in Liberia). Data were analyzed with regard to % positivity, % agreement, and heterogeneity. Models evaluated potential effects of age, gender, and blood microfilaria (Mf) counts in individuals and effects of endemicity and history of MDA at the village level as potential factors linked to higher sensitivity of the FTS. Lastly, we assessed relationships between CFA scores and Mf in pre- and post-MDA settings. PRINCIPAL FINDINGS: Paired test results were available for 3,682 individuals. Antigenemia rates were 8% and 22% higher by FTS than by ICT in pre-MDA and in post-MDA sites, respectively. FTS/ICT ratios were higher in areas with low infection rates. The probability of having microfilaremia was much higher in persons with CFA scores >1 in untreated areas. However, this was not true in post-MDA settings. CONCLUSIONS/SIGNIFICANCE: This study has provided extensive new information on the performance of the FTS compared to ICT in Africa and it has confirmed the increased sensitivity of FTS reported in prior studies. Variability in FTS/ICT was related in part to endemicity level, history of MDA, and perhaps to the medications used for MDA. These results suggest that FTS should be superior to ICT for mapping, for transmission assessment surveys, and for post-MDA surveillance.
[Mh] Termos MeSH primário: Antígenos de Helmintos/sangue
Filariose Linfática/diagnóstico
Sistemas Automatizados de Assistência Junto ao Leito
Wuchereria bancrofti/isolamento & purificação
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Criança
Filariose Linfática/epidemiologia
Filariose Linfática/transmissão
Feminino
Seres Humanos
Imunocromatografia
Masculino
Microfilárias/isolamento & purificação
Meia-Idade
Wuchereria bancrofti/química
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Antigens, Helminth)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005703


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[PMID]:28891597
[Au] Autor:Kanamitie JN; Ahorlu CS; Otchere J; Aboagye-Antwi F; Kwansa-Bentum B; Boakye DA; Biritwum NK; Wilson MD; de Souza DK
[Ad] Endereço:Department of Animal Biology and Conservation Science, University of Ghana, Accra, Ghana.
[Ti] Título:Twelve-month longitudinal parasitological assessment of lymphatic filariasis-positive individuals: impact of a biannual treatment with ivermectin and albendazole.
[So] Source:Trop Med Int Health;22(11):1451-1456, 2017 Nov.
[Is] ISSN:1365-3156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Mass drug administration (MDA) for the control of lymphatic filariasis (LF), in Ghana, started in the year 2000. While this had great success in many implementation units, there remain areas with persistent transmission, after more than 10 years of treatment. A closer examination of the parasite populations could help understand the reasons for persistent infections and formulate appropriate strategies to control LF in these areas of persistent transmission. MATERIALS AND METHODS: In a longitudinal study, we assessed the prevalence of microfilaraemia (mf) in two communities with 12 years of MDA in Ghana. In baseline surveys 6 months after the National MDA in 2014, 370 consenting individuals were tested for antigenaemia using immunochromatographic test (ICT) cards and had their mf count determined through night blood surveys. 48 ICT positives, of whom, 17 were positive for mf, were treated with 400 µg/kg ivermectin + 400 mg albendazole and subsequently followed for parasitological assessment at 3-month intervals for 1 year. This overlapped with the National MDA in 2015. RESULTS: There was a 68% parasite clearance 3 months after treatment. The pre-treatment mf count differed significantly from the post-treatment mf counts at 3 months (P = 0.0023), 6 months (P = 0.0051), 9 months (P = 0.0113) and 12 months (P = 0.0008). CONCLUSION: In these settings with persistent LF transmission, twice-yearly treatment may help accelerate LF elimination. Further large-scale evaluations are required to ascertain these findings.
[Mh] Termos MeSH primário: Albendazol/uso terapêutico
Filariose Linfática/parasitologia
Filaricidas/uso terapêutico
Filarioidea/crescimento & desenvolvimento
Ivermectina/uso terapêutico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Albendazol/farmacologia
Animais
Antígenos de Helmintos/sangue
Criança
Filariose Linfática/sangue
Filariose Linfática/epidemiologia
Filariose Linfática/prevenção & controle
Feminino
Filaricidas/farmacologia
Filarioidea/efeitos dos fármacos
Gana/epidemiologia
Programas Governamentais
Seres Humanos
Ivermectina/farmacologia
Estudos Longitudinais
Masculino
Microfilárias/efeitos dos fármacos
Microfilárias/crescimento & desenvolvimento
Meia-Idade
Prevalência
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Helminth); 0 (Filaricides); 70288-86-7 (Ivermectin); F4216019LN (Albendazole)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE
[do] DOI:10.1111/tmi.12974


  5 / 2245 MEDLINE  
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[PMID]:28406927
[Au] Autor:Subramanian S; Jambulingam P; Chu BK; Sadanandane C; Vasuki V; Srividya A; Mohideen AbdulKader MS; Krishnamoorthy K; Raju HK; Laney SJ; Williams SA; Henderson RH
[Ad] Endereço:Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry, India.
[Ti] Título:Application of a household-based molecular xenomonitoring strategy to evaluate the lymphatic filariasis elimination program in Tamil Nadu, India.
[So] Source:PLoS Negl Trop Dis;11(4):e0005519, 2017 Apr.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The monitoring and evaluation of lymphatic filariasis (LF) has largely relied on the detection of antigenemia and antibodies in human populations. Molecular xenomonitoring (MX), the detection of parasite DNA/RNA in mosquitoes, may be an effective complementary method, particularly for detecting signals in low-level prevalence areas where Culex is the primary mosquito vector. This paper investigated the application of a household-based sampling method for MX in Tamil Nadu, India. METHODS: MX surveys were conducted in 2010 in two evaluation units (EUs): 1) a hotspot area, defined as sites with community microfilaria prevalence ≥1%, and 2) a larger area that also encompassed the hotspots. Households were systematically selected using a sampling interval proportional to the number of households in the EU. Mosquito pools were collected and analyzed by real-time polymerase chain reaction (qPCR). Two independent samples were taken in each EU to assess reproducibility of results. Follow-up surveys were conducted in 2012. RESULTS: In 2010, the proportion of positive pools in the hotspot EU was 49.3% compared to 23.4% in the overall EU. In 2012, pool positivity was significantly reduced to 24.3% and 6.5%, respectively (p<0.0001). Pool positivity based on independent samples taken from each EU in 2010 and 2012 were not significantly different except for the hotspot EU in 2012 (p = 0.009). The estimated prevalence of infection in mosquitoes, measured by PoolScreen, declined from 2.2-2.7% in 2010 to 0.6-1.2% in 2012 in the hotspot area and from 0.9-1.1% to 0.2-0.3% in the larger area. CONCLUSIONS: The household-based sampling strategy for MX led to mostly reproducible results and supported the observed LF infection trends found in humans. MX has the potential to be a cost-effective, non-invasive monitoring and evaluation tool with sensitive detection of infection signals in low prevalence settings. Further investigation and application of this sampling strategy for MX are recommended to support its adoption as a standardized method for global LF elimination programs.
[Mh] Termos MeSH primário: Controle de Doenças Transmissíveis/métodos
Culex/parasitologia
Filariose Linfática/epidemiologia
Wuchereria bancrofti/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
DNA de Helmintos/isolamento & purificação
Filariose Linfática/prevenção & controle
Características da Família
Seres Humanos
Índia/epidemiologia
Microfilárias/genética
Microfilárias/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real
Wuchereria bancrofti/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005519


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[PMID]:28369129
[Au] Autor:Modi A; Gamit S; Jesalpura BS; Kurien G; Kosambiya JK
[Ad] Endereço:Department of Community Medicine, Government Medical College, Surat, Gujarat, India.
[Ti] Título:Reaching endpoints for lymphatic filariasis elimination- results from mass drug administration and nocturnal blood surveys, South Gujarat, India.
[So] Source:PLoS Negl Trop Dis;11(4):e0005476, 2017 Apr.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Following the World Health Assembly resolution on Elimination of lymphatic filariasis (ELF) as a public health problem by the year 2020, a Global Program (GPELF) was launched in 1997 to help endemic countries to initiate national programs. The current strategy to interrupt transmission of LF, is administration of once-yearly, single-dose, two-drug regimen (Albendazole with Diethylcarbamazine (DEC) to be used in endemic areas with the goal of reaching 65% epidemiological coverage for 4-6 years. We report findings of independent assessment from year 2010 to 2015 for last six rounds, after initial five rounds of Mass Drug Administration (MDA) since 2005 for ELF in endemic area of Gujarat. METHODS: Independent assessment of MDA was performed to find coverage and compliance indicators, reasons for non-coverage and non-compliance in five Implementation Units (IUs). Pre, during and post MDA evaluations were done in three phases. The impact of MDA was measured by microfilaraemia survey. A total of eight sites, four random and four fixed sentinel sites were selected to calculate microfilaria rate (MF) per IUs per year. In years 2010 to 2015, we report results from 125,936 nocturnal blood smears and 17551 population in 120 selected clusters. Four clusters were selected per year in each of the five IUs for assessment of MDA round. RESULT: Post MDA survey showed drug coverage between 81%-88% and epidemiological coverage 77%-89% across years. Main reasons for non-coverage were drug administrator related (the team did not visit or missed people) while non-compliance was population related (fear of side effects, sickness, people forgot or absent). During MDA findings show that the directly observed consumption is considerably improved from 58% in 2010 to 82% in 2015. The knowledge about benefits of drug provided also increased from 59% to 90% over the years. The current MF rate is less than one in all IUs with an overall 68% percent decrease from baseline year 2005 to year 2015. The average MF rate of Gujarat is 0.44 for year 2015. CONCLUSIONS: The findings show that achieving adequate epidemiological and drug coverage is possible by actual field level operation of the program in large endemic areas. The results and feedback from independent assessment, performed regularly, could guide the policymakers and program managers for mid-term corrections and to frame strategies to enhance program. Monitoring of coverage and impact indicator together informs decisions for reaching end-point of MDA. The impact indicator- microfilaria rate in all IUs of South Gujarat Region has reached and remained less than one percent signaling end-points of MDA. Post MDA stringent monitoring in form of TAS is recommended to keep vigil on maintenance of elimination achieved.
[Mh] Termos MeSH primário: Albendazol/administração & dosagem
Dietilcarbamazina/administração & dosagem
Filariose Linfática/prevenção & controle
Filaricidas/administração & dosagem
Wuchereria bancrofti/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Erradicação de Doenças
Quimioterapia Combinada
Filariose Linfática/tratamento farmacológico
Filariose Linfática/epidemiologia
Doenças Endêmicas
Pesquisa sobre Serviços de Saúde
Seres Humanos
Índia/epidemiologia
Microfilárias
Avaliação de Programas e Projetos de Saúde
Vigilância de Evento Sentinela
Inquéritos e Questionários
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Filaricides); F4216019LN (Albendazole); V867Q8X3ZD (Diethylcarbamazine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005476


  7 / 2245 MEDLINE  
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[PMID]:28288690
[Au] Autor:Alho AM; Marcelino I; Colella V; Flanagan C; Silva N; Correia JJ; Latrofa MS; Otranto D; Madeira de Carvalho L
[Ad] Endereço:CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa (ULisboa), Lisboa, Portugal.
[Ti] Título:Dirofilaria immitis in pinnipeds and a new host record.
[So] Source:Parasit Vectors;10(1):142, 2017 Mar 13.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Dirofilaria immitis is a mosquito-borne pathogen that is spreading worldwide, and the associated infection (i.e. dirofilariosis) is becoming a threat to animals and humans living in endemic areas. Little is known about the occurrence and risk of infection of D. immitis in pinnipeds. Here we report dirofilariosis by D. immitis in several pinniped species kept in captivity in Portugal. METHODS: Animals were housed in an oceanographic park located in Algarve, southern Portugal, a geographical area endemic for canine dirofilariosis. To assess the occurrence of D. immitis, blood was collected from the park's resident pinniped population, which consisted of 16 animals (5 common seals Phoca vitulina, 2 grey seals Halichoerus grypus, 3 California sea lions Zalophus californianus and 6 South African fur seals Arctocephalus pusillus pusillus). Dirofilaria immitis nematodes were detected by real-time PCR and by the presence of circulating antigens. In addition, modified Knott's technique was performed to detect circulating microfilariae. Necropsies and histopathological examination of two animals which died during the study were also conducted. RESULTS: Out of the 16 pinnipeds housed at the park, seven (43.8%) were positive for D. immitis by real-time PCR (3 P. vitulina, 2 Z. californianus and 2 A. p. pusillus), two of which (P. vitulina) were also positive for the nematode's antigen. Additionally, D. immitis microfilariae were detected in one A. p. pusillus. Furthermore, several D. immitis specimens were retrieved from the right ventricle and pulmonary arteries at the necropsy of one P. vitulina and one A. p. pusillus. CONCLUSIONS: This study provides new epidemiological data on D. immitis infection in pinnipeds diagnosed through clinical, molecular and pathological findings. Additionally, the South African fur seal is herein reported as a new host for this zoonotic filarioid. The situation herein described could also occur in other parks located in areas where canine dirofilariosis is endemic. Active surveillance and preventive measures of dirofilariosis in pinnipeds on a local and global scale are therefore vital to improve the early diagnosis and control of dirofilariosis.
[Mh] Termos MeSH primário: Caniformia/parasitologia
Dirofilaria immitis/isolamento & purificação
Dirofilariose/epidemiologia
[Mh] Termos MeSH secundário: Animais
Antígenos de Helmintos/sangue
Antígenos de Helmintos/imunologia
Dirofilaria immitis/genética
Dirofilariose/diagnóstico
Dirofilariose/parasitologia
Microfilárias/isolamento & purificação
Portugal/epidemiologia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Helminth)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2073-0


  8 / 2245 MEDLINE  
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[PMID]:28232044
[Au] Autor:Mircean M; Ionica AM; Mircean V; Györke A; Codea AR; Tabaran FA; Taulescu M; Dumitrache MO
[Ad] Endereço:Department of Internal Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur 3-5, Cluj-Napoca 400372, Cluj, Romania.
[Ti] Título:Clinical and pathological effects of Dirofilaria repens and Dirofilaria immitis in a dog with a natural co-infection.
[So] Source:Parasitol Int;66(3):331-334, 2017 Jun.
[Is] ISSN:1873-0329
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Canine dirofilarioses are mosquito-borne zoonotic diseases with a continuous expansion of their geographical distribution, as a consequence of different climatic and ecological factors. Dirofilaria immitis, the aetiological agent of heartworm disease, has gained a major veterinary interest, mainly due to its severe clinical implication. In the last decades, D. repens, despite of being regarded as a less pathogenic species, regained attention due to its recognized zoonotic potential. Romania has been traditionally regarded as a non-endemic country, but recent epidemiological surveys are highlighting the presence of both D. repens and D. immitis. The present case report describes the clinical and pathological features of a natural co-infection with D. repens and D. immitis in 5year old male Boxer from north-western Romania. Based on clinical and laboratory investigations, a diagnosis of kidney failure and chronical cystitis was established. The parasites were identified by morphology and confirmed by PCR and sequencing. To the best of our knowledge this is the first worldwide report of the ectopic presence of D. repens adults in the pelvic cavity and mesentery by natural infection and first report of the ocular localization of this parasite for Romania. The clinical and paraclinical findings suggest the implication of D. repens microfilariae in the aetiology of histopathological lesions. Further investigations are needed in order to establish the actual pathogenic potential of D. repens, a frequently neglected parasite.
[Mh] Termos MeSH primário: Coinfecção/veterinária
Dirofilaria immitis/isolamento & purificação
Dirofilaria repens/isolamento & purificação
Dirofilariose/diagnóstico
Dirofilariose/parasitologia
Doenças do Cão/parasitologia
[Mh] Termos MeSH secundário: Animais
Coinfecção/parasitologia
Dirofilaria immitis/genética
Dirofilaria repens/genética
Dirofilaria repens/patogenicidade
Dirofilariose/epidemiologia
Doenças do Cão/diagnóstico
Cães
Ecocardiografia
Masculino
Microfilárias/isolamento & purificação
Reação em Cadeia da Polimerase
Insuficiência Renal/parasitologia
Romênia/epidemiologia
Zoonoses/parasitologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170510
[Lr] Data última revisão:
170510
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE


  9 / 2245 MEDLINE  
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[PMID]:28222120
[Au] Autor:Vaughan JA; Turell MJ
[Ad] Endereço:Virology Division, U.S. Army Medical Research Institute for Infectious Diseases, Fort Detrick, Maryland, United States of America.
[Ti] Título:Brugia malayi microfilariae transport alphaviruses across the mosquito midgut.
[So] Source:PLoS One;12(2):e0172309, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Concurrent ingestion of microfilariae (MF) and arboviruses by mosquitoes can enhance mosquito transmission of virus compared to when virus is ingested alone. Within hours of being ingested, MF penetrate the mosquito midgut and introduce virus into mosquito hemocoel, creating a disseminated viral infection much sooner than normal. How virus is actually introduced is not known. In this report, we present experimental evidence that suggests that certain alphaviruses may adhere or otherwise associate with sheathed Brugia malayi MF in the blood of a dually-infected host and that the virus is carried into the mosquito hemocoel by the MF during their penetration of the mosquito midgut. The mechanism of MF enhancement may be more complex than simple leakage of viremic blood into the hemocoel during MF penetration. The affinity of arboviruses to adhere to or otherwise associate with MF may depend on the specific combination of the virus and MF involved in a dual host infection. This in turn may determine the relative importance that MF enhancement has within an arbovirus transmission system.
[Mh] Termos MeSH primário: Aedes/virologia
Brugia Malayi/fisiologia
Vírus da Encefalite Equina do Leste
Vírus da Encefalite Equina Venezuelana
Filariose/transmissão
Insetos Vetores/virologia
Intestinos/parasitologia
Intestinos/virologia
Microfilárias/fisiologia
[Mh] Termos MeSH secundário: Aedes/parasitologia
Aedes/fisiologia
Animais
Transporte Biológico
Sangue/parasitologia
Sangue/virologia
Suscetibilidade a Doenças
Vírus da Encefalite Equina do Leste/fisiologia
Vírus da Encefalite Equina Venezuelana/fisiologia
Comportamento Alimentar
Filariose/parasitologia
Filariose/virologia
Gerbillinae
Insetos Vetores/parasitologia
Insetos Vetores/fisiologia
Glândulas Salivares/virologia
Especificidade da Espécie
Viremia/transmissão
Viremia/virologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172309


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[PMID]:28045905
[Au] Autor:McCoy CJ; Reaves BJ; Giguère S; Coates R; Rada B; Wolstenholme AJ
[Ad] Endereço:Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, United States of America.
[Ti] Título:Human Leukocytes Kill Brugia malayi Microfilariae Independently of DNA-Based Extracellular Trap Release.
[So] Source:PLoS Negl Trop Dis;11(1):e0005279, 2017 Jan.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Wuchereria bancrofti, Brugia malayi and Brugia timori infect over 100 million people worldwide and are the causative agents of lymphatic filariasis. Some parasite carriers are amicrofilaremic whilst others facilitate mosquito-based disease transmission through blood-circulating microfilariae (Mf). Recent findings, obtained largely from animal model systems, suggest that polymorphonuclear leukocytes (PMNs) contribute to parasitic nematode-directed type 2 immune responses. When exposed to certain pathogens PMNs release extracellular traps (NETs) in the form of chromatin loaded with various antimicrobial molecules and proteases. PRINCIPAL FINDINGS: In vitro, PMNs expel large amounts of NETs that capture but do not kill B. malayi Mf. NET morphology was confirmed by fluorescence imaging of worm-NET aggregates labelled with DAPI and antibodies to human neutrophil elastase, myeloperoxidase and citrullinated histone H4. A fluorescent, extracellular DNA release assay was used to quantify and observe Mf induced NETosis over time. Blinded video analyses of PMN-to-worm attachment and worm survival during Mf-leukocyte co-culture demonstrated that DNase treatment eliminates PMN attachment in the absence of serum, autologous serum bolsters both PMN attachment and PMN plus peripheral blood mononuclear cell (PBMC) mediated Mf killing, and serum heat inactivation inhibits both PMN attachment and Mf killing. Despite the effects of heat inactivation, the complement inhibitor compstatin did not impede Mf killing and had little effect on PMN attachment. Both human PMNs and monocytes, but not lymphocytes, are able to kill B. malayi Mf in vitro and NETosis does not significantly contribute to this killing. Leukocytes derived from presumably parasite-naïve U.S. resident donors vary in their ability to kill Mf in vitro, which may reflect the pathological heterogeneity associated with filarial parasitic infections. CONCLUSIONS/SIGNIFICANCE: Human innate immune cells are able to recognize, attach to and kill B. malayi microfilariae in an in vitro system. This suggests that, in vivo, the parasites can evade this ability, or that only some human hosts support an infection with circulating Mf.
[Mh] Termos MeSH primário: Brugia Malayi/imunologia
DNA de Helmintos/imunologia
Armadilhas Extracelulares/imunologia
Filariose/imunologia
Microfilárias/imunologia
Neutrófilos/imunologia
[Mh] Termos MeSH secundário: Animais
Brugia Malayi/genética
Brugia Malayi/fisiologia
DNA de Helmintos/genética
Armadilhas Extracelulares/parasitologia
Filariose/parasitologia
Gerbillinae
Seres Humanos
Leucócitos Mononucleares/imunologia
Microfilárias/genética
Microfilárias/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005279



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