Base de dados : MEDLINE
Pesquisa : B01.050.500.500.294.400.937.463.700 [Categoria DeCS]
Referências encontradas : 174 [refinar]
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  1 / 174 MEDLINE  
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[PMID]:29320823
[Au] Autor:Shin J; Ahn KS; Suh GH; Kim HJ; Jeong HS; Kim BS; Choi E; Shin SS
[Ad] Endereço:Department of Parasitology, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
[Ti] Título:First Blindness Cases of Horses Infected with Setaria Digitata (Nematoda: Filarioidea) in the Republic of Korea.
[So] Source:Korean J Parasitol;55(6):667-671, 2017 Dec.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Ocular setariases of cattle were reported but those of equine hosts have never been reported in the Republic of Korea (Korea). We found motile worms in the aqueous humor of 15 horses (Equus spp.) from 12 localities in southern parts of Korea between January 2004 and November 2017. After the affected animals were properly restrained under sedation and local anesthesia, 10 ml disposable syringe with a 16-gauge needle was inserted into the anterior chamber of the affected eye to successfully remove the parasites. The male worm that was found in 7 of the cases showed a pair of lateral appendages near the posterior terminal end of the body. The papillar arrangement was 3 pairs of precloacal, a pair of adcloacal, and 3 pairs of postcloacal papillae, plus a central papilla just in front of the cloaca. The female worms found in the eyes of 8 horses were characterized by the tapering posterior terminal end of the body with a smooth knob. Worms were all identified as Setaria digitata (von Linstow, 1906) by the morphologic characteristics using light and electron microscopic observations. This is the first blindness cases of 15 horses infected with S. digitata (Nematoda: Filarioidea) in Korea.
[Mh] Termos MeSH primário: Cegueira/etiologia
Cegueira/veterinária
Doenças dos Cavalos/etiologia
Doenças dos Cavalos/parasitologia
Cavalos
Procedimentos Cirúrgicos Oftalmológicos/veterinária
Setaria (Nematoide)/isolamento & purificação
Setaríase/complicações
Setaríase/parasitologia
[Mh] Termos MeSH secundário: Animais
Cegueira/cirurgia
Feminino
Doenças dos Cavalos/cirurgia
Masculino
Microscopia Eletrônica
Procedimentos Cirúrgicos Oftalmológicos/métodos
República da Coreia
Setaria (Nematoide)/anatomia & histologia
Setaria (Nematoide)/ultraestrutura
Setaríase/cirurgia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.6.667


  2 / 174 MEDLINE  
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[PMID]:29273505
[Au] Autor:Wadhawan M; Tiwari S; Sharma S; Rathaur S
[Ad] Endereço:Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi, India.
[Ti] Título:Identification and characterization of a novel prolyl oligopeptidase in filarial parasite Setaria cervi.
[So] Source:Biochem Biophys Res Commun;495(3):2235-2241, 2018 01 15.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A 75 kDa serine protease having prolyl oligopeptidase activity has been purified from Setaria cervi, a bovine filarial parasite. The MALDI-MS/MS analysis of the purified protein revealed 6 peptides showing nearest match S9A (prolyl oligopeptidase) family protein from Plesiocystis pacifica. The ScPOP was found to be unique compared to mammalian POP with respect to its kinetic properties. To elucidate its role, filarial parasites were exposed to specific inhibitor of POP, Z-Pro-prolinal (ZPP) for 8 h. The inhibition of POP induced calcium signaling via phospholipase c stimulation which further triggered mitochondrial mediated apoptosis in filarial parasites.
[Mh] Termos MeSH primário: Apoptose/fisiologia
Sinalização do Cálcio/fisiologia
Mitocôndrias/enzimologia
Peptídeo Hidrolases/química
Peptídeo Hidrolases/metabolismo
Setaria (Nematoide)/enzimologia
Setaria (Nematoide)/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sítios de Ligação
Ativação Enzimática
Ligação Proteica
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.4.- (Peptide Hydrolases); EC 3.4.- (oligopeptidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171224
[St] Status:MEDLINE


  3 / 174 MEDLINE  
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[PMID]:28351683
[Au] Autor:Senathilake KS; Karunanayake EH; Samarakoon SR; Tennekoon KH; de Silva ED; Adhikari A
[Ad] Endereço:Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo 03, Sri Lanka.
[Ti] Título:Oleanolic acid from antifilarial triterpene saponins of Dipterocarpus zeylanicus induces oxidative stress and apoptosis in filarial parasite Setaria digitata in vitro.
[So] Source:Exp Parasitol;177:13-21, 2017 Jun.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Absence of a drug that kills adult filarial parasites remains the major challenge in eliminating human lymphatic filariasis (LF); the second leading cause of long-term and permanent disability. Thus, the discovery of novel antifilarial natural products with potent adulticidal activity is an urgent need. In the present study, methanol extracts of leaves, bark and winged seeds of Dipterocarpus zeylanicus (Dipterocarpaceae) were investigated for macro and microfilaricidal activity. Two antifilarial triterpene saponins were isolated from winged seed extracts by bioactivity guided chromatographic separation and identified using Nuclear Magnetic Resonance and mass spectroscopic analysis as oleanolic acid 3-O-ß-D- glucopyranoside (1) (IC50 = 20.54 µM for adult worms, 19.71 µM for microfilariae ) and oleanolic acid 3-O-α-L-arabinopyranoside (2) (IC50 = 29.02 µM for adult worms, 25.99 µM for microfilariae). Acid hydrolysis of both compounds yielded oleanolic acid (3) which was non or least toxic to human peripheral blood mono nuclear cells (Selectivity index = >10) while retaining similar macrofilaricidal (IC50 = 38.4 µM) and microfilaricidal (IC50 = 35.6 µM) activities. In adult female worms treated with 50 and 100 µM doses of oleanolic acid, condensation of nuclear DNA, apoptotic body formation and tissue damage was observed by using Hoechst 33342 staining, TUNEL assay and Hematoxylin and Eosin staining respectively. A dose dependent increase in caspase 3/CED3 activity and decrease in total protein content were also observed in these parasites. A dose dependant DNA fragmentation was observed in adult parasites and microfilariae. Decreased levels of reduced glutathione (GSH) and elevated levels of glutathione S transferase (GST), superoxide dismutase (SOD) and reactive oxygen species (ROS) were also observed in parasites treated with oleanolic acid indicating an oxidative stress mediated apoptotic event. Compound 3/oleanolic acid was thus identified as a potent and safe antifilarial compound in vitro.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Embriófitas/química
Ácido Oleanólico/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Saponinas/química
Setaria (Nematoide)/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Caspase 3/metabolismo
Fragmentação do DNA/efeitos dos fármacos
Relação Dose-Resposta a Droga
Feminino
Marcação In Situ das Extremidades Cortadas
Concentração Inibidora 50
Dose Letal Mediana
Ácido Oleanólico/isolamento & purificação
Casca de Planta/química
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacologia
Folhas de Planta/química
Espécies Reativas de Oxigênio/metabolismo
Sementes/química
Setaria (Nematoide)/citologia
Setaria (Nematoide)/metabolismo
Setaríase/tratamento farmacológico
Setaríase/parasitologia
Coloração e Rotulagem
Triterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Reactive Oxygen Species); 0 (Saponins); 0 (Triterpenes); 6SMK8R7TGJ (Oleanolic Acid); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE


  4 / 174 MEDLINE  
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[PMID]:28030352
[Au] Autor:Priyadarshi P; Dravid P; Sheikh IH; Saxena S; Tandon A; Kaushal DC; Ali S; Kaushal NA
[Ti] Título:Characterization of immunoreactive proteins of Setaria cervi isolated by preparative polyacrylamide gel electrophoresis.
[So] Source:Acta Parasitol;62(1):29-37, 2017 Mar 01.
[Is] ISSN:1896-1851
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Filarial parasites are complex mixtures of antigenic proteins and characterization of these antigenic molecules is essential to identify the diagnostically important filaria-specific antigens. In the present study, we have fractionated the somatic extracts from adults of Setaria cervi (bovine filarial parasite) on preparative SDS-polyacrylamide gel electrophoresis and tested the immunoreactivity of the separated gel fractions with polyclonal antibodies against filarial excretory-secretory antigens as well as filarial patients sera. The SDS-PAGE analysis of gel eluted fractions revealed 1 protein band in F-1 fraction, 2 protein bands in F-2 fraction and 2-3 protein bands in all other fractions (F3- F11). Seven gel eluted fractions (F1, F2, F3, F4, F5, F6 and F11) showed high ELISA reactivity with the polyclonal antibody (against excretory-secretory antigen) and four of these fractions (F-1, F-2, F3 and F6) exhibited high ELISA reactivity with antibodies present in filarial patient sera. The reactivities of the gel fractions (F1 and F2), recognized by filarial patients sera, were also tested with the monoclonal antibody (detecting the filarial circulating antigen). The F1 and F2 gel eluted fractions were found to have the target antigen of monoclonal antibody as evident by high reactivity with the monoclonal antibody in ELISA and immunoblotting. The S. cervi gel eluted F1 fraction (containing single antigen) could detect antibodies in filarial patients sera and not in non-filarial sera thereby suggesting its usefulness for specific serodiagnosis of human filariasis.
[Mh] Termos MeSH primário: Antígenos de Helmintos/imunologia
Eletroforese em Gel de Poliacrilamida
Proteínas de Helminto/metabolismo
Setaria (Nematoide)/metabolismo
[Mh] Termos MeSH secundário: Animais
Anticorpos Anti-Helmínticos
Ensaio de Imunoadsorção Enzimática/métodos
Feminino
Proteínas de Helminto/genética
Seres Humanos
Soros Imunes
Testes Imunológicos
Masculino
Camundongos
Coelhos
Setaríase/sangue
Setaríase/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Helminth Proteins); 0 (Immune Sera)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170517
[Lr] Data última revisão:
170517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE


  5 / 174 MEDLINE  
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[PMID]:27794504
[Au] Autor:Nabie R; Spotin A; Rouhani S
[Ad] Endereço:Nikookari Eye Center, Tabriz University of Medical Sciences, Tabriz, Iran.
[Ti] Título:Subconjunctival setariasis due to Setaria equina infection; a case report and a literature review.
[So] Source:Parasitol Int;66(1):930-932, 2017 Feb.
[Is] ISSN:1873-0329
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A rare case of human subconjunctival setariasis due to Setaria equina infection is reported herein. A 15-years old girl was referred with a 24h history of edema and redness in her left eye. On slit lamp examination, a thread-like cylindrical worm was moving in the subconjunctival area. The worm was extracted, stained and measured 110mm in length 510µm in width. The isolated worm was identified as adult female S. equina based on morphometric criteria. Identification of the species of the worm was confirmed using molecular methods. For this purpose, the 12S rRNA gene was PCR-amplified and the purified amplicon was directly sequenced. After alignment, phylogenetic analysis revealed that the 12S rRNA sequence of this worm (Accession no.: KU291446) showed 100% identity with that of S. equina. This is the first case in Iran and provides evidence that S. equina can be an etiological agent of subconjunctival infection was isolated and diagnosed as where it located Middle East.
[Mh] Termos MeSH primário: Túnica Conjuntiva/parasitologia
Infecções Oculares Parasitárias/parasitologia
Setaria (Nematoide)/isolamento & purificação
Setaríase/parasitologia
[Mh] Termos MeSH secundário: Adolescente
Animais
Infecções Oculares Parasitárias/diagnóstico
Feminino
Seres Humanos
Irã (Geográfico)
Filogenia
Reação em Cadeia da Polimerase
RNA Ribossômico
Alinhamento de Sequência
Análise de Sequência de DNA
Setaríase/diagnóstico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (RNA, Ribosomal); 0 (RNA, ribosomal, 12S)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


  6 / 174 MEDLINE  
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[PMID]:27788338
[Au] Autor:Gomez-Puerta LA; Mayor P
[Ad] Endereço:Department of Veterinary Epidemiology and Economics, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru.
[Ti] Título:Congenital Filariasis Caused by Setaria bidentata (Nematoda: Filarioidea) in the Red Brocket Deer (Mazama americana).
[So] Source:J Parasitol;103(1):123-126, 2017 Feb.
[Is] ISSN:1937-2345
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The filarial nematode Setaria bidentata was found in 10 of 31 fetuses of the red brocket deer ( Mazama americana ) from the Loreto region of the Peruvian Amazon. A total of 25 specimens were collected and morphologically identified as S. bidentata. Filarial nematodes were found in the peritoneal cavity of 9 deer fetuses and the thoracic cavity of 1 fetus. Most specimens were adult stage. In this report, we provide morphometric data for these filarial specimens. This is the first study to demonstrate prenatal S. bidentata infection in cervid fetuses. Also, the finding of S. bidentata in Peru expands the geographic range of this parasite.
[Mh] Termos MeSH primário: Cervos/parasitologia
Doenças Fetais/veterinária
Setaria (Nematoide)
Setaríase/congênito
[Mh] Termos MeSH secundário: Animais
Cervos/embriologia
Feminino
Doenças Fetais/parasitologia
Masculino
Cavidade Peritoneal/embriologia
Cavidade Peritoneal/parasitologia
Peru
Setaria (Nematoide)/anatomia & histologia
Setaria (Nematoide)/isolamento & purificação
Setaríase/parasitologia
Cavidade Torácica/embriologia
Cavidade Torácica/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161028
[St] Status:MEDLINE
[do] DOI:10.1645/16-86


  7 / 174 MEDLINE  
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[PMID]:27659561
[Au] Autor:Mukherjee S; Mukherjee S; Bhattacharya S; Sinha Babu SP
[Ad] Endereço:Department of Zoology, Centre for Advanced Studies, Visva-Bharati University, Santiniketan, West Bengal, India.
[Ti] Título:Surface proteins of Setaria cervi induce inflammation in macrophage through Toll-like receptor 4 (TLR4)-mediated signalling pathway.
[So] Source:Parasite Immunol;39(1), 2017 Jan.
[Is] ISSN:1365-3024
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lymphatic filariasis is a vectorborne parasitic disease that results in morbidities, disabilities and socio-economic loss each year globally. Inflammatory consequences associated with any form of filariasis have drawn special attention. However, the molecular insight behind the inflammation of host macrophage (MФ) is considered as one of the shaded areas in filarial research. Herein, major emphasis was given to study the signalling pathway of MФ inflammation induced by surface proteins (SPs) of filarial parasite through in vitro and in vivo approaches. Twenty-four hours of in vitro stimulation of Raw MФs with endotoxin-free SPs of Setaria cervi resulted in the secretion of pro-inflammatory cytokines (TNF-α and IL-1ß) that revealed induction of inflammation, which was found to be elicited from classical NF-кB activation. Moreover, this NF-кB activation was found to be signalled from TLR4 and mediated by the downstream signalling intermediates, viz. MyD88, pTAK1 and NEMO. In vivo studies in adult Wistar rats, experimentally injected with SPs, clearly supported the outcomes of in vitro experiments by showing higher degree of inflammation rather classical activation of the peritoneal MФs. Therefore, SPs from S. cervi cuticle could be responsible for the induction of pro-inflammatory response in MФ, which appears to be propagated through TLR4-NF-кB route.
[Mh] Termos MeSH primário: Proteínas de Helminto/imunologia
Inflamação/parasitologia
Macrófagos/imunologia
Setaria (Nematoide)/imunologia
Transdução de Sinais
Receptor 4 Toll-Like/metabolismo
[Mh] Termos MeSH secundário: Animais
Bovinos
Linhagem Celular Tumoral
Células Cultivadas
Feminino
Inflamação/metabolismo
Interleucina-1beta/metabolismo
Macrófagos/metabolismo
Masculino
Proteínas de Membrana/metabolismo
Camundongos
Ratos
Ratos Wistar
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Helminth Proteins); 0 (Interleukin-1beta); 0 (Membrane Proteins); 0 (Toll-Like Receptor 4); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160924
[St] Status:MEDLINE
[do] DOI:10.1111/pim.12389


  8 / 174 MEDLINE  
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[PMID]:27174667
[Au] Autor:Senathilake KS; Karunanayake EH; Samarakoon SR; Tennekoon KH; de Silva ED
[Ad] Endereço:Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo 03, Sri Lanka. Electronic address: senathilake@gmail.com.
[Ti] Título:Rhizome extracts of Curcuma zedoaria Rosc induce caspase dependant apoptosis via generation of reactive oxygen species in filarial parasite Setaria digitata in vitro.
[So] Source:Exp Parasitol;167:50-60, 2016 Aug.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human lymphatic filariasis (LF) is mainly caused by filarial parasite Wuchereria bancrofti and is the second leading cause of long term and permanent disability in tropical countries. To date, incapability to eliminate long lived adult parasites by current drugs remains the major challenge in the elimination of LF. Hence, in the current study, the efficacy of rhizome extracts of Curcuma zedoaria (a plant traditionally used in Sri Lanka in the management of LF) was evaluated as an effective filaricide in vitro. Sequential solvent extracts of C. zedoaria rhizomes were screened for in vitro antifilarial activity at 0.01-1 mg/mL concentrations by motility inhibition assay and 3-(4, 5 dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide (MTT) reduction assay using cattle parasite Setaria digitata as a model organism. Exposure of parasites to hexane and chloroform extracts of C. zedoaria caused a dose dependant reduction in motility and viability of microfilariae (IC50 = 72.42 µg/mL for hexane extract, 191.14 µg/mL for chloroform extract) and adult parasites (IC50 = 77.07 µg/mL for hexane extract, 259.87 µg/mL for chloroform extract). Both extracts were less toxic to human peripheral blood mononuclear cells when compared to filariae. A dose dependant increase in caspase 3/CED 3 and a decrease in total protein content, cyclooxygenase (COX) and protein tyrosine phosphatase (PTP) activities were observed in adult parasites treated with hexane or chloroform extract. A significant degree of chromatin condensation and apoptotic body formation were also observed in these worms by Hoechst 33342 and terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) staining respectively. Dose dependant chromosomal DNA laddering was observed in treated adult worms but not in microfilariae in response to both extracts. Oxidative stress parameters such as reduction in reduced glutathione (GSH) levels and increase in glutathione s transferase (GST), superoxide dismutase (SOD) and catalase activities, increased reactive oxygen levels (ROS) and lipid peroxidation were also observed indicating that an apoptotic event is induced by reactive oxygen species.
[Mh] Termos MeSH primário: Curcuma/química
Extratos Vegetais/farmacologia
Piroptose/efeitos dos fármacos
Espécies Reativas de Oxigênio/metabolismo
Rizoma/química
Setaria (Nematoide)/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Caspase 3/metabolismo
Bovinos
Fragmentação do DNA
Relação Dose-Resposta a Droga
Feminino
Seres Humanos
Marcação In Situ das Extremidades Cortadas
Concentração Inibidora 50
Leucócitos Mononucleares/efeitos dos fármacos
Cavidade Peritoneal/parasitologia
Extratos Vegetais/toxicidade
Setaria (Nematoide)/citologia
Setaria (Nematoide)/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Reactive Oxygen Species); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160514
[St] Status:MEDLINE


  9 / 174 MEDLINE  
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[PMID]:27093573
[Au] Autor:Abdel-Latif M; Sakran T
[Ad] Endereço:a Immunology Division, Zoology Department , Beni-Suef University , Beni-Suef , Egypt.
[Ti] Título:Detection for cross-reactive proteins in filarial worm Setaria equina, MCF-7 human breast cancer, and Huh-7 hepatoma cells.
[So] Source:J Immunoassay Immunochem;37(6):572-84, 2016.
[Is] ISSN:1532-4230
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study aimed to detect the cross-reactive proteins in filarial parasite adult worm Setaria equina and two different tumor cell lines (MCF-7 human breast cancer and Huh-7 hepatoma cells). This was performed using rabbit anti-S. equina extract (SeqE) or DEC (Diethylcarbamazine citrate) polyclonal IgG antibodies by indirect ELISA and western blotting. The results indicated cross-reactive bands at 70 and 75 kDa in all extracts by anti-DEC and SeqE antibodies, respectively. In addition, the expression of 70 kDa protein was only reduced in filarial worms and Huh-7 after in vitro DEC treatment compared to the control.
[Mh] Termos MeSH primário: Antígenos/imunologia
Setaria (Nematoide)/química
Setaria (Nematoide)/imunologia
[Mh] Termos MeSH secundário: Animais
Antígenos de Neoplasias/imunologia
Western Blotting
Reações Cruzadas
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Imunoglobulina G/imunologia
Células MCF-7
Masculino
Coelhos
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens); 0 (Antigens, Neoplasm); 0 (Immunoglobulin G)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160420
[St] Status:MEDLINE
[do] DOI:10.1080/15321819.2016.1179644


  10 / 174 MEDLINE  
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[PMID]:26965172
[Au] Autor:Singh N; Wadhawan M; Tiwari S; Kumar R; Rathaur S
[Ad] Endereço:Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
[Ti] Título:Inhibition of Setaria cervi protein tyrosine phosphatases by Phenylarsine oxide: A proteomic and biochemical study.
[So] Source:Acta Trop;159:20-8, 2016 Jul.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Phenylarsine oxide (PAO), a specific protein tyrosine phosphatase (PTP) inhibitor significantly decreased the motility and viability of Setaria cervi ultimately leading to its death. The PTP activity present in the cytosolic and detergent soluble fractions as well as on surface of these parasites was significantly inhibited by PAO. A marked alteration in protein spots abundance after proteomic analysis showed 14 down-regulated and 9 upregulated spots in the treated parasites as compared to the control. The PTP inhibition led to increase in the cytosolic and mitochondrial calpain activity in these parasites. PAO also blocked the ATP generation in the parasite depicted by reduced activity of phosphoglycerate kinase and expression of enolase. An increased ROS level, induced lipid peroxidation/protein carbonyl formation and decreased activity of different antioxidant enzymes like thioredoxin reductase, glutathione reductase and glutathione transferases was also observed in the PAO treated parasites. PAO, thus disturbs the overall homeostasis of the filarial parasite by inhibiting PTPs. Thereby suggesting that these molecules could be used as a good chemotherapeutic target for lymphatic filariasis.
[Mh] Termos MeSH primário: Arsenicais/metabolismo
Inibidores Enzimáticos/metabolismo
Proteínas de Helminto/metabolismo
Proteínas Tirosina Fosfatases/metabolismo
Setaria (Nematoide)/enzimologia
Setaríase/prevenção & controle
Setaríase/parasitologia
[Mh] Termos MeSH secundário: Animais
Búfalos/parasitologia
Índia
Proteômica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arsenicals); 0 (Enzyme Inhibitors); 0 (Helminth Proteins); 0HUR2WY345 (oxophenylarsine); EC 3.1.3.48 (Protein Tyrosine Phosphatases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160312
[St] Status:MEDLINE



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