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Pesquisa : B01.050.500.500.294.400.968.100.100 [Categoria DeCS]
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[PMID]:28692668
[Au] Autor:Papaiakovou M; Pilotte N; Grant JR; Traub RJ; Llewellyn S; McCarthy JS; Krolewiecki AJ; Cimino R; Mejia R; Williams SA
[Ad] Endereço:Department of Biological Sciences, Smith College, Northampton, Massachusetts, United States of America.
[Ti] Título:A novel, species-specific, real-time PCR assay for the detection of the emerging zoonotic parasite Ancylostoma ceylanicum in human stool.
[So] Source:PLoS Negl Trop Dis;11(7):e0005734, 2017 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Molecular-based surveys have indicated that Ancylostoma ceylanicum, a zoonotic hookworm, is likely the second most prevalent hookworm species infecting humans in Asia. Most current PCR-based diagnostic options for the detection of Ancylostoma species target the Internal Transcribed Spacer (ITS) regions of the ribosomal gene cluster. These regions possess a considerable degree of conservation among the species of this genus and this conservation can lead to the misidentification of infecting species or require additional labor for accurate species-level determination. We have developed a novel, real-time PCR-based assay for the sensitive and species-specific detection of A. ceylanicum that targets a non-coding, highly repetitive genomic DNA element. Comparative testing of this PCR assay with an assay that targets ITS sequences was conducted on field-collected samples from Argentina and Timor-Leste to provide further evidence of the sensitivity and species-specificity of this assay. METHODS/PRINCIPAL FINDINGS: A previously described platform for the design of primers/probe targeting non-coding highly repetitive regions was used for the development of this novel assay. The assay's limits of detection (sensitivity) and cross-reactivity with other soil-transmitted helminth species (specificity) were assessed with real-time PCR experiments. The assay was successfully used to identify infections caused by A. ceylanicum that were previously only identified to the genus level as Ancylostoma spp. when analyzed using other published primer-probe pairings. Further proof of sensitive, species-specific detection was provided using a published, semi-nested restriction fragment length polymorphism-PCR assay that differentiates between Ancylostoma species. CONCLUSIONS/SIGNIFICANCE: Due to the close proximity of people and domestic/wild animals in many regions of the world, the potential for zoonotic infections is substantial. Sensitive tools enabling the screening for different soil-transmitted helminth infections are essential to the success of mass deworming efforts and facilitate the appropriate interpretation of data. This study describes a novel, species-specific, real-time PCR-based assay for the detection of A. ceylanicum that will help to address the need for such tools in integrated STH deworming programs. TRIAL REGISTRATION: ANZCTR.org.au ACTRN12614000680662.
[Mh] Termos MeSH primário: Ancylostoma/isolamento & purificação
Ancilostomíase/diagnóstico
Fezes/parasitologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Ancylostoma/classificação
Animais
Argentina
DNA de Helmintos/isolamento & purificação
Seres Humanos
Filogenia
Polimorfismo de Fragmento de Restrição
Ensaios Clínicos Controlados Aleatórios como Assunto
Especificidade da Espécie
Timor-Leste
Zoonoses/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005734


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[PMID]:28647243
[Au] Autor:Gyawali P; Ahmed W; Sidhu JP; Jagals P; Toze S
[Ad] Endereço:CSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, Qld 4102, Australia; School of Public Health, The University of Queensland, Herston Road, Qld 4006, Australia. Electronic address: pradep033@yahoo.com.
[Ti] Título:Quantification of hookworm ova from wastewater matrices using quantitative PCR.
[So] Source:J Environ Sci (China);57:231-237, 2017 Jul.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A quantitative PCR (qPCR) assay was used to quantify Ancylostoma caninum ova in wastewater and sludge samples. We estimated the average gene copy numbers for a single ovum using a mixed population of ova. The average gene copy numbers derived from the mixed population were used to estimate numbers of hookworm ova in A. caninum seeded and unseeded wastewater and sludge samples. The newly developed qPCR assay estimated an average of 3.7×10 gene copies per ovum, which was then validated by seeding known numbers of hookworm ova into treated wastewater. The qPCR estimated an average of (1.1±0.1), (8.6±2.9) and (67.3±10.4) ova for treated wastewater that was seeded with (1±0), (10±2) and (100±21) ova, respectively. The further application of the qPCR assay for the quantification of A. caninum ova was determined by seeding a known numbers of ova into the wastewater matrices. The qPCR results indicated that 50%, 90% and 67% of treated wastewater (1L), raw wastewater (1L) and sludge (~4g) samples had variable numbers of A. caninum gene copies. After conversion of the qPCR estimated gene copy numbers to ova for treated wastewater, raw wastewater, and sludge samples, had an average of 0.02, 1.24 and 67 ova, respectively. The result of this study indicated that qPCR can be used for the quantification of hookworm ova from wastewater and sludge samples; however, caution is advised in interpreting qPCR generated data for health risk assessment.
[Mh] Termos MeSH primário: Ancylostoma
Monitoramento Ambiental/métodos
Óvulo
Águas Residuais/parasitologia
[Mh] Termos MeSH secundário: Animais
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170626
[St] Status:MEDLINE


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[PMID]:28617281
[Au] Autor:Gyawali P; Sidhu JPS; Ahmed W; Jagals P; Toze S
[Ad] Endereço:School of Public Health, The University of Queensland, Herston Road, Herston, Qld 4006, Australia E-mail: pradep033@yahoo.com.
[Ti] Título:Comparison of culture-based, vital stain and PMA-qPCR methods for the quantitative detection of viable hookworm ova.
[So] Source:Water Sci Technol;75(11-12):2615-2621, 2017 Jun.
[Is] ISSN:0273-1223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Accurate quantitative measurement of viable hookworm ova from environmental samples is the key to controlling hookworm re-infections in the endemic regions. In this study, the accuracy of three quantitative detection methods [culture-based, vital stain and propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR)] was evaluated by enumerating 1,000 ± 50 Ancylostoma caninum ova in the laboratory. The culture-based method was able to quantify an average of 397 ± 59 viable hookworm ova. Similarly, vital stain and PMA-qPCR methods quantified 644 ± 87 and 587 ± 91 viable ova, respectively. The numbers of viable ova estimated by the culture-based method were significantly (P < 0.05) lower than vital stain and PMA-qPCR methods. Therefore, both PMA-qPCR and vital stain methods appear to be suitable for the quantitative detection of viable hookworm ova. However, PMA-qPCR would be preferable over the vital stain method in scenarios where ova speciation is needed.
[Mh] Termos MeSH primário: Ancylostoma/isolamento & purificação
Azidas/metabolismo
Azul de Metileno/química
Parasitologia/métodos
Propídio/análogos & derivados
Reação em Cadeia da Polimerase em Tempo Real/métodos
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Animais
Óvulo
Parasitologia/instrumentação
Propídio/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azides); 0 (propidium monoazide); 36015-30-2 (Propidium); T42P99266K (Methylene Blue)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.2166/wst.2017.142


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[PMID]:28408213
[Au] Autor:Tielemans E; Lebon W; Dumont P; Taweethavonsawat P; Larsen D; Rehbein S
[Ad] Endereço:Merial SAS, Centre de Recherche de Saint-Vulbas, 850 allée des Cyprès, 01150 Saint Vulbas, France.
[Ti] Título:Efficacy of afoxolaner plus milbemycin oxime chewable tablets (NexGard Spectra , Merial) against adult Ancylostoma ceylanicum hookworm, in dogs.
[So] Source:Vet Parasitol;238:87-89, 2017 Apr 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A fixed-combination chewable tablet incorporating afoxolaner plus milbemycin oxime (NexGard Spectra , Merial) was tested in purpose-bred Beagle dogs for efficacy against adult Ancylostoma ceylanicum hookworms. Sixteen dogs were inoculated each by oral administration of approximately 500 infective larvae of A. ceylanicum. Seventeen days after inoculation, the dogs were weighed and allocated randomly to be treated with afoxolaner plus milbemycin oxime chewable tablets or to remain untreated. Commercial chewable tablets of different strength were combined to deliver doses as close as possible to the minimum effective dose of 2.5mg afoxolaner plus 0.5mg milbemycin oxime per kg body weight. Parasites were recovered and counted for determination of efficacy seven days after treatment. All eight dogs that had been left untreated were harboring adult A. ceylanicum (geometric mean, 317.8; range, 210-428) while only one and nine A. ceylanicum were recovered from two of the eight dogs treated with afoxolaner plus milbemycin oxime chewable tablets (geometric mean, 0.5; p<0.0001). Thus, 99.9% efficacy against induced infection of A. ceylanicum was obtained by the use of oral NexGard Spectra at the minimum effective dose. Treatment with afoxolaner plus milbemycin oxime chewable tablets was well accepted and safe.
[Mh] Termos MeSH primário: Ancylostoma/efeitos dos fármacos
Ancilostomíase/veterinária
Doenças do Cão/parasitologia
Isoxazóis/uso terapêutico
Macrolídeos/uso terapêutico
Naftalenos/uso terapêutico
[Mh] Termos MeSH secundário: Ancilostomíase/tratamento farmacológico
Animais
Anti-Helmínticos/administração & dosagem
Anti-Helmínticos/uso terapêutico
Doenças do Cão/tratamento farmacológico
Cães
Combinação de Medicamentos
Feminino
Isoxazóis/administração & dosagem
Macrolídeos/administração & dosagem
Masculino
Naftalenos/administração & dosagem
Comprimidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Drug Combinations); 0 (Isoxazoles); 0 (Macrolides); 0 (Naphthalenes); 0 (Tablets); 02L07H6D0U (afoxolaner); 0502PUN0GT (milbemycin oxime)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE


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[PMID]:28395755
[Au] Autor:Geurden T; Vatta AF; Slootmans N; King VL; Lin D; McTier T; Rugg D
[Ad] Endereço:Zoetis, Veterinary Medicine Research and Development, Mercuriusstraat 20, B-1930 Zaventem, Belgium. Electronic address: thomas.geurden@zoetis.com.
[Ti] Título:Efficacy of a new spot-on formulation of selamectin plus sarolaner against Ancylostoma tubaeforme and Toxocara cati in cats.
[So] Source:Vet Parasitol;238 Suppl 1:S31-S35, 2017 Apr.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The efficacy of a new spot-on formulation of selamectin plus sarolaner for cats was evaluated against induced infections with Ancylostoma tubaeforme (hookworm) and Toxocara cati (roundworm). Five laboratory studies were conducted using adult, purpose-bred cats. Four of the studies were designed to evaluate efficacy of the combination against A. tubaeforme, the dose-limiting gastrointestinal nematode species for selamectin. In two of these studies non-interference between selamectin and sarolaner was also evaluated. The fifth study evaluated efficacy of the combination against mixed infections of A. tubaeforme and T. cati. The hookworm isolates in three studies were of US origin, as was the roundworm isolate. In the two remaining studies, cats were inoculated with a hookworm isolate of European origin. Cats were inoculated with 150 (±50) to 200 (±50) infective hookworm larvae 30-42days prior to treatment and with 400 infective roundworm eggs 60days prior to treatment. Cats were ranked by pre-treatment faecal egg counts and randomly allocated to different treatment groups. In all studies, cats were treated at the minimum label dose to provide 6.0mg selamectin per kg bodyweight. All animals were euthanized 7-10days after treatment for worm counts. Efficacy was calculated based on the reduction of the geometric mean worm counts in the treated groups versus the placebo-treated control groups. The efficacy against adult hookworms was 99.2%, 94.3% and 100% in three of these studies, and was lower in the remaining two studies. The efficacy against T. cati was 100%. Furthermore, non-interference between sarolaner and selamectin was demonstrated. Thus, a single topical application of the new spot-on formulation of selamectin plus sarolaner at the minimum label dose is effective in the treatment of adult hookworm and roundworm infections in cats.
[Mh] Termos MeSH primário: Ancilostomíase/veterinária
Doenças do Gato/tratamento farmacológico
Isoxazóis/administração & dosagem
Ivermectina/análogos & derivados
Toxocaríase/tratamento farmacológico
[Mh] Termos MeSH secundário: Ancylostoma
Ancilostomíase/tratamento farmacológico
Animais
Antiparasitários/administração & dosagem
Gatos
Feminino
Ivermectina/administração & dosagem
Masculino
Distribuição Aleatória
Toxocara
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiparasitic Agents); 0 (Isoxazoles); 70288-86-7 (Ivermectin); A2669OWX9N (selamectin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170418
[Lr] Data última revisão:
170418
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE


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[PMID]:28214519
[Au] Autor:Amoah ID; Singh G; Stenström TA; Reddy P
[Ad] Endereço:Institute for Water and Wastewater Technology (IWWT), Durban University of Technology, PO Box 1334, Durban, 4000, South Africa; Department of Community Health Studies, Faculty of Health Sciences Durban University of Technology, PO Box 1334, Durban, 4000, South Africa. Electronic address: amoahkid@gm
[Ti] Título:Detection and quantification of soil-transmitted helminths in environmental samples: A review of current state-of-the-art and future perspectives.
[So] Source:Acta Trop;169:187-201, 2017 May.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:It is estimated that over a billion people are infected with soil-transmitted helminths (STHs) globally with majority occurring in tropical and subtropical regions of the world. The roundworm (Ascaris lumbricoides), whipworm (Trichuris trichiura), and hookworms (Ancylostoma duodenale and Necator americanus) are the main species infecting people. These infections are mostly gained through exposure to faecally contaminated water, soil or contaminated food and with an increase in the risk of infections due to wastewater and sludge reuse in agriculture. Different methods have been developed for the detection and quantification of STHs eggs in environmental samples. However, there is a lack of a universally accepted technique which creates a challenge for comparative assessments of helminths egg concentrations both in different samples matrices as well as between locations. This review presents a comparison of reported methodologies for the detection of STHs eggs, an assessment of the relative performance of available detection methods and a discussion of new emerging techniques that could be applied for detection and quantification. It is based on a literature search using PubMed and Science Direct considering all geographical locations. Original research articles were selected based on their methodology and results sections. Methods reported in these articles were grouped into conventional, molecular and emerging techniques, the main steps in each method were then compared and discussed. The inclusion of a dissociation step aimed at detaching helminth eggs from particulate matter was found to improve the recovery of eggs. Additionally the selection and application of flotation solutions that take into account the relative densities of the eggs of different species of STHs also results in higher egg recovery. Generally the use of conventional methods was shown to be laborious and time consuming and prone to human error. The alternate use of nucleic acid-based techniques has improved the sensitivity of detection and made species specific identification possible. However, these nucleic acid based methods are expensive and less suitable in regions with limited resources and skill. The loop mediated isothermal amplification method shows promise for application in these settings due to its simplicity and use of basic equipment. In addition, the development of imaging soft-ware for the detection and quantification of STHs shows promise to further reduce human error associated with the analysis of environmental samples. It may be concluded that there is a need to comparatively assess the performance of different methods to determine their applicability in different settings as well as for use with different sample matrices (wastewater, sludge, compost, soil, vegetables etc.).
[Mh] Termos MeSH primário: Helmintíase/parasitologia
Helmintos/isolamento & purificação
Solo/parasitologia
[Mh] Termos MeSH secundário: Ancylostoma
Animais
Ascaris lumbricoides/isolamento & purificação
Fezes/parasitologia
Seres Humanos
Necator americanus/isolamento & purificação
Trichuris/isolamento & purificação
Águas Residuais/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Soil); 0 (Waste Water)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170220
[St] Status:MEDLINE


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[PMID]:28077747
[Au] Autor:Pa Pa Aung W; Htoon TT; Tin HH; Sanpool O; Jongthawin J; Sadaow L; Phosuk I; Ropai R; Intapan PM; Maleewong W
[Ad] Endereço:Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
[Ti] Título:First Molecular Identifications of Necator americanus and Ancylostoma ceylanicum Infecting Rural Communities in Lower Myanmar.
[So] Source:Am J Trop Med Hyg;96(1):214-216, 2017 Jan 11.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hookworms are enteric parasitic roundworms infecting an estimated 400 million persons worldwide. Herein, we provide the first molecular identifications of human hookworms from certain parts of rural Lower Myanmar. DNA was extracted from hookworm-positive stool samples, as determined by microscopy. DNA sequences of the partial internal transcribed spacer 1, full length 5.8S gene, and partial internal transcribed spacer 2 were determined and compared with available hookworm sequences from public databases. Of the 11 polymerase chain reaction-positive samples, eight (Bago Region, N = 4; Mon State, N = 4) yielded sequences with high similarity to those of Necator americanus A further three sequences (Mon State, N = 2; Bago Region, N = 1) showed high similarity with those of Ancylostoma ceylanicum The latter is primarily a parasite of dogs and represents a zoonosis. Given that different species of hookworms exhibit different epidemiological and biological characteristics, accurate identification is essential for the planning and execution of effective control programs for hookworm infections.
[Mh] Termos MeSH primário: Ancylostoma/isolamento & purificação
Necator americanus/isolamento & purificação
Necatoríase/epidemiologia
Necatoríase/parasitologia
[Mh] Termos MeSH secundário: Animais
DNA de Helmintos/genética
DNA de Helmintos/isolamento & purificação
Fezes/parasitologia
Seres Humanos
Mianmar/epidemiologia
Reação em Cadeia da Polimerase
População Rural
Especificidade da Espécie
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0610


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[PMID]:28074315
[Au] Autor:Strkolcová G; Goldová M; Bocková E; Mojzisová J
[Ad] Endereço:Department of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Kosice, Slovak Republic. gabriela.strkolcova@uvlf.sk.
[Ti] Título:The roundworm Strongyloides stercoralis in children, dogs, and soil inside and outside a segregated settlement in Eastern Slovakia: frequent but hardly detectable parasite.
[So] Source:Parasitol Res;116(3):891-900, 2017 Mar.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A comparative study was carried out to evaluate the Strongyloides stercoralis infections in children and dogs inside and outside the segregated settlement in Medzev, Eastern Slovakia, and a survey of the soil within the settlement was included. Applying the Koga agar plate (KAP) culture method and microscopy examination of stool samples collected from 60 Roma and 21 nonRoma children, no larvae of S. stercoralis were detected but eggs of three nematodes (Ascaris lumbricoides, Trichuris trichiura, and Enterobius vermicularis) and cysts of two protozoan endoparasites (Giardia duodenalis and Cryptosporidium spp.) were often found. However, immunoenzymatic assay (ELISA) for the evidence of IgG antibodies against S. stercoralis showed 33.3% seroprevalence in Roma children and 23.8% prevalence in children from the majority population, attending the same school. Eosinophilia was regularly present in children with exclusive infection of S. stercoralis (eight cases) as well as in individuals suffering from mixed infections of S. stercoralis and some of the above listed parasites (16 cases); high eosinophil counts sometimes, but not always, occurred in parasitized children lacking S. stercoralis antibodies. A comparison of S. stercoralis in dogs from the settlement (40 dogs) and from a distant dog shelter (20 dogs) did not reveal remarkable differences: the direct microscopy of faecal samples revealed rhabditiform larvae in 13.3% of the dogs from the settlement (4/30) and in 10.0% of the dogs from the shelter (2/20). Out of blood samples collected from the second dog group, 55% of the dogs contained antibodies against S. stercoralis. In the soil collected from 14 various locations within the settlement, S. stercoralis larvae were observed in two samples (14.3%); however, 13 samples (92.9%) were positive for human or dog endoparasites of the genera Ancylostoma, Ascaris, Toxocara, Toxascaris, Trichuris, and Hymenolepis.
[Mh] Termos MeSH primário: Doenças do Cão/parasitologia
Solo/parasitologia
Strongyloides stercoralis/isolamento & purificação
Estrongiloidíase/parasitologia
[Mh] Termos MeSH secundário: Ágar
Ancylostoma/genética
Ancylostoma/isolamento & purificação
Ancylostoma/fisiologia
Animais
Ascaris
Ascaris lumbricoides/genética
Ascaris lumbricoides/isolamento & purificação
Ascaris lumbricoides/fisiologia
Criança
Pré-Escolar
Coinfecção
Doenças do Cão/epidemiologia
Cães
Enterobius
Ensaio de Imunoadsorção Enzimática
Fezes/parasitologia
Feminino
Giardia lamblia/genética
Giardia lamblia/isolamento & purificação
Giardia lamblia/fisiologia
Seres Humanos
Masculino
Prevalência
Estudos Soroepidemiológicos
Eslováquia/epidemiologia
Strongyloides stercoralis/classificação
Strongyloides stercoralis/genética
Estrongiloidíase/epidemiologia
Toxocara/genética
Toxocara/isolamento & purificação
Toxocara/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 9002-18-0 (Agar)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-016-5362-1


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[PMID]:26752269
[Au] Autor:Oliveira-Arbex AP; David EB; Oliveira-Sequeira TC; Katagiri S; Coradi ST; Guimarães S
[Ad] Endereço:UNESP - Universidade Estadual Paulista, Departamento de Parasitologia, Instituto de Biociências,CEP: 18618-970,Botucatu, São Paulo,Brazil.
[Ti] Título:Molecular identification of Ancylostoma species from dogs and an assessment of zoonotic risk in low-income households, São Paulo State, Brazil.
[So] Source:J Helminthol;91(1):14-19, 2017 Jan.
[Is] ISSN:1475-2697
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hookworm infection stands out for its worldwide distribution and for its veterinary and public health relevance. Based on copromicroscopic examinations and polymerase chain reaction (PCR) amplification of the ITS1-5.8S-ITS2 region, we assessed, respectively, the prevalence of intestinal parasites and the identification of canine hookworm species in faeces recovered from 278 dogs living in households of an inland municipality of São Paulo State, Brazil. Intestinal parasites were found in 67.3% of dogs and hookworm infection was found at the highest prevalence rate (56.6%), followed by Toxocara canis (11.9%), Isospora spp. (11.9%), Giardia spp. (5.8%), Sarcocystis spp. (4.0%), 'Hammondia-like' (1.4%), Dipylidium caninum (1.1%) and Trichuris vulpis (0.7%). Of 158 samples positive for hookworm eggs, 106 (67.1%) were amplified by PCR and, of those, 88 (55.7%) were successfully sequenced for species identification. Single infections with Ancylostoma caninum and Ancylostoma braziliense were recorded in 61.4% and 12.5%, respectively, and mixed infections were found in 26.1%. The nucleotide sequences of both species showed high identity rates (98-100%) when compared with reference sequences. Although A. caninum was the most prevalent hookworm in the dogs assessed, the occurrence of both A. caninum and A. braziliense in single and/or mixed infections poses a potential risk for the local population in a low-income area, especially children, to acquire cutaneous larva migrans (CLM).
[Mh] Termos MeSH primário: Ancylostoma/isolamento & purificação
Ancilostomíase/veterinária
Transmissão de Doença Infecciosa
Doenças do Cão/diagnóstico
Doenças do Cão/parasitologia
Zoonoses/epidemiologia
Zoonoses/transmissão
[Mh] Termos MeSH secundário: Ancylostoma/genética
Ancilostomíase/diagnóstico
Ancilostomíase/epidemiologia
Ancilostomíase/parasitologia
Animais
Brasil
DNA de Helmintos/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Doenças do Cão/epidemiologia
Doenças do Cão/transmissão
Cães
Características da Família
Saúde da Família
Fezes/parasitologia
Renda
Microscopia
Técnicas de Diagnóstico Molecular
Reação em Cadeia da Polimerase
Prevalência
Medição de Risco
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160112
[St] Status:MEDLINE
[do] DOI:10.1017/S0022149X15001145


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[PMID]:27751868
[Au] Autor:Weeks JC; Roberts WM; Robinson KJ; Keaney M; Vermeire JJ; Urban JF; Lockery SR; Hawdon JM
[Ad] Endereço:Institute of Neuroscience and African Studies Program, University of Oregon, 1254 University of Oregon, Eugene, OR 97403-1254, USA. Electronic address: jweeks@uoregon.edu.
[Ti] Título:Microfluidic platform for electrophysiological recordings from host-stage hookworm and Ascaris suum larvae: A new tool for anthelmintic research.
[So] Source:Int J Parasitol Drugs Drug Resist;6(3):314-328, 2016 12.
[Is] ISSN:2211-3207
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The screening of candidate compounds and natural products for anthelmintic activity is important for discovering new drugs against human and animal parasites. We previously validated in Caenorhabditis elegans a microfluidic device ('chip') that records non-invasively the tiny electrophysiological signals generated by rhythmic contraction (pumping) of the worm's pharynx. These electropharyngeograms (EPGs) are recorded simultaneously from multiple worms per chip, providing a medium-throughput readout of muscular and neural activity that is especially useful for compounds targeting neurotransmitter receptors and ion channels. Microfluidic technologies have transformed C. elegans research and the goal of the current study was to validate hookworm and Ascaris suum host-stage larvae in the microfluidic EPG platform. Ancylostoma ceylanicum and A. caninum infective L3s (iL3s) that had been activated in vitro generally produced erratic EPG activity under the conditions tested. In contrast, A. ceylanicum L4s recovered from hamsters exhibited robust, sustained EPG activity, consisting of three waveforms: (1) conventional pumps as seen in other nematodes; (2) rapid voltage deflections, associated with irregular contractions of the esophagus and openings of the esophogeal-intestinal valve (termed a 'flutter'); and (3) hybrid waveforms, which we classified as pumps. For data analysis, pumps and flutters were combined and termed EPG 'events.' EPG waveform identification and analysis were performed semi-automatically using custom-designed software. The neuromodulator serotonin (5-hydroxytryptamine; 5HT) increased EPG event frequency in A. ceylanicum L4s at an optimal concentration of 0.5 mM. The anthelmintic drug ivermectin (IVM) inhibited EPG activity in a concentration-dependent manner. EPGs from A. suum L3s recovered from pig lungs exhibited robust pharyngeal pumping in 1 mM 5HT, which was inhibited by IVM. These experiments validate the use of A. ceylanicum L4s and A. suum L3s with the microfluidic EPG platform, providing a new tool for screening anthelmintic candidates or investigating parasitic nematode feeding behavior.
[Mh] Termos MeSH primário: Ancylostoma/fisiologia
Anti-Helmínticos/farmacologia
Ascaris suum/fisiologia
Avaliação Pré-Clínica de Medicamentos/métodos
Fenômenos Eletrofisiológicos/efeitos dos fármacos
Microfluídica/métodos
[Mh] Termos MeSH secundário: Ancylostoma/efeitos dos fármacos
Animais
Ascaris suum/efeitos dos fármacos
Larva/efeitos dos fármacos
Larva/fisiologia
Parasitologia/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Anthelmintics)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161019
[St] Status:MEDLINE



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