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[PMID]:29216182
[Au] Autor:Chauhan VM; Scurr DJ; Christie T; Telford G; Aylott JW; Pritchard DI
[Ad] Endereço:School of Pharmacy, Boots Science Building, University of Nottingham, Nottingham, United Kingdom.
[Ti] Título:The physicochemical fingerprint of Necator americanus.
[So] Source:PLoS Negl Trop Dis;11(12):e0005971, 2017 Dec.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Necator americanus, a haematophagous hookworm parasite, infects ~10% of the world's population and is considered to be a significant public health risk. Its lifecycle has distinct stages, permitting its successful transit from the skin via the lungs (L3) to the intestinal tract (L4 maturing to adult). It has been hypothesised that the L3 larval sheath, which is shed during percutaneous infection (exsheathment), diverts the immune system to allow successful infection and reinfection in endemic areas. However, the physicochemical properties of the L3 larval cuticle and sheath, which are in direct contact with the skin and its immune defences, are unknown. In the present study, we controlled exsheathment, to characterise the sheath and underlying cuticle surfaces in situ, using atomic force microscopy (AFM) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). AFM revealed previously unseen surface area enhancing nano-annuli exclusive to the sheath surface and confirmed greater adhesion forces exist between cationic surfaces and the sheath, when compared to the emergent L3 cuticle. Furthermore, ToF-SIMS elucidated different chemistries between the surfaces of the cuticle and sheath which could be of biological significance. For example, the phosphatidylglycerol rich cuticle surface may support the onward migration of a lubricated infective stage, while the anionic and potentially immunologically active heparan sulphate rich deposited sheath could result in the diversion of immune defences to an inanimate antigenic nidus. We propose that our initial studies into the surface analysis of this hookworm provides a timely insight into the physicochemical properties of a globally important human pathogen at its infective stage and anticipate that the development and application of this analytical methodology will support translation of these findings into a biological context.
[Mh] Termos MeSH primário: Larva/anatomia & histologia
Larva/química
Necator americanus/anatomia & histologia
Necator americanus/química
[Mh] Termos MeSH secundário: Animais
Antígenos de Helmintos/química
Espectrometria de Massas
Análise Multivariada
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Helminth)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005971


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[PMID]:28489889
[Au] Autor:Wardell R; Clements ACA; Lal A; Summers D; Llewellyn S; Campbell SJ; McCarthy J; Gray DJ; V Nery S
[Ad] Endereço:Department of Global Health, Research School of Population Health, College of Medicine, Biology and the Environment, The Australian National University, Canberra, ACT, Australia.
[Ti] Título:An environmental assessment and risk map of Ascaris lumbricoides and Necator americanus distributions in Manufahi District, Timor-Leste.
[So] Source:PLoS Negl Trop Dis;11(5):e0005565, 2017 May.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In Timor-Leste there have been intermittent and ineffective soil-transmitted helminth (STH) deworming programs since 2004. In a resource-constrained setting, having information on the geographic distribution of STH can aid in prioritising high risk communities for intervention. This study aimed to quantify the environmental risk factors for STH infection and to produce a risk map of STH in Manufahi district, Timor-Leste. METHODOLOGY/PRINCIPAL FINDINGS: Georeferenced cross-sectional data and stool samples were obtained from 2,194 participants in 606 households in 24 villages in the Manufahi District as part of cross sectional surveys done in the context of the "WASH for Worms" randomised controlled trial. Infection status was determined for Ascaris lumbricoides and Necator americanus using real-time quantitative polymerase chain reaction. Baseline infection data were linked to environmental data obtained for each household. Univariable and multivariable multilevel mixed-effects logistic regression analysis with random effects at the village and household level were conducted, with all models adjusted for age and sex. For A. lumbricoides, being a school-aged child increased the odds of infection, whilst higher temperatures in the coolest quarter of the year, alkaline soils, clay loam/loam soils and woody savannas around households were associated with decreased infection odds. For N. americanus, greater precipitation in the driest month, higher average enhanced vegetation index, age and sandy loam soils increased infection odds, whereas being female and living at higher elevations decreased the odds of infection. Predictive risk maps generated for Manufahi based upon these final models highlight the high predicted risk of N. americanus infection across the district and the more focal nature of A. lumbricoides infection. The predicted risk of any STH infection is high across the entire district. CONCLUSIONS/SIGNIFICANCE: The widespread predicted risk of any STH infection in 6 to 18 year olds provides strong evidence to support strategies for control across the entire geographical area. As few studies include soil texture and pH in their analysis, this study adds to a growing body of evidence suggesting these factors influence STH infection distribution. This study also further supports that A. lumbricoides prefers acidic soils, highlighting a potential relatively unexplored avenue for control. TRIAL REGISTRATION: ClinicalTrials.gov ACTRN12614000680662.
[Mh] Termos MeSH primário: Ascaríase/epidemiologia
Ascaris lumbricoides/isolamento & purificação
Exposição Ambiental
Necator americanus/isolamento & purificação
Necatoríase/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Ascaríase/parasitologia
Criança
Pré-Escolar
Estudos Transversais
Fezes/parasitologia
Feminino
Seres Humanos
Lactente
Masculino
Meia-Idade
Necatoríase/parasitologia
Ensaios Clínicos Controlados Aleatórios como Assunto
Medição de Risco
Timor-Leste/epidemiologia
Topografia Médica
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170511
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005565


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[PMID]:28214519
[Au] Autor:Amoah ID; Singh G; Stenström TA; Reddy P
[Ad] Endereço:Institute for Water and Wastewater Technology (IWWT), Durban University of Technology, PO Box 1334, Durban, 4000, South Africa; Department of Community Health Studies, Faculty of Health Sciences Durban University of Technology, PO Box 1334, Durban, 4000, South Africa. Electronic address: amoahkid@gm
[Ti] Título:Detection and quantification of soil-transmitted helminths in environmental samples: A review of current state-of-the-art and future perspectives.
[So] Source:Acta Trop;169:187-201, 2017 May.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:It is estimated that over a billion people are infected with soil-transmitted helminths (STHs) globally with majority occurring in tropical and subtropical regions of the world. The roundworm (Ascaris lumbricoides), whipworm (Trichuris trichiura), and hookworms (Ancylostoma duodenale and Necator americanus) are the main species infecting people. These infections are mostly gained through exposure to faecally contaminated water, soil or contaminated food and with an increase in the risk of infections due to wastewater and sludge reuse in agriculture. Different methods have been developed for the detection and quantification of STHs eggs in environmental samples. However, there is a lack of a universally accepted technique which creates a challenge for comparative assessments of helminths egg concentrations both in different samples matrices as well as between locations. This review presents a comparison of reported methodologies for the detection of STHs eggs, an assessment of the relative performance of available detection methods and a discussion of new emerging techniques that could be applied for detection and quantification. It is based on a literature search using PubMed and Science Direct considering all geographical locations. Original research articles were selected based on their methodology and results sections. Methods reported in these articles were grouped into conventional, molecular and emerging techniques, the main steps in each method were then compared and discussed. The inclusion of a dissociation step aimed at detaching helminth eggs from particulate matter was found to improve the recovery of eggs. Additionally the selection and application of flotation solutions that take into account the relative densities of the eggs of different species of STHs also results in higher egg recovery. Generally the use of conventional methods was shown to be laborious and time consuming and prone to human error. The alternate use of nucleic acid-based techniques has improved the sensitivity of detection and made species specific identification possible. However, these nucleic acid based methods are expensive and less suitable in regions with limited resources and skill. The loop mediated isothermal amplification method shows promise for application in these settings due to its simplicity and use of basic equipment. In addition, the development of imaging soft-ware for the detection and quantification of STHs shows promise to further reduce human error associated with the analysis of environmental samples. It may be concluded that there is a need to comparatively assess the performance of different methods to determine their applicability in different settings as well as for use with different sample matrices (wastewater, sludge, compost, soil, vegetables etc.).
[Mh] Termos MeSH primário: Helmintíase/parasitologia
Helmintos/isolamento & purificação
Solo/parasitologia
[Mh] Termos MeSH secundário: Ancylostoma
Animais
Ascaris lumbricoides/isolamento & purificação
Fezes/parasitologia
Seres Humanos
Necator americanus/isolamento & purificação
Trichuris/isolamento & purificação
Águas Residuais/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Soil); 0 (Waste Water)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170220
[St] Status:MEDLINE


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[PMID]:28077747
[Au] Autor:Pa Pa Aung W; Htoon TT; Tin HH; Sanpool O; Jongthawin J; Sadaow L; Phosuk I; Ropai R; Intapan PM; Maleewong W
[Ad] Endereço:Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
[Ti] Título:First Molecular Identifications of Necator americanus and Ancylostoma ceylanicum Infecting Rural Communities in Lower Myanmar.
[So] Source:Am J Trop Med Hyg;96(1):214-216, 2017 Jan 11.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hookworms are enteric parasitic roundworms infecting an estimated 400 million persons worldwide. Herein, we provide the first molecular identifications of human hookworms from certain parts of rural Lower Myanmar. DNA was extracted from hookworm-positive stool samples, as determined by microscopy. DNA sequences of the partial internal transcribed spacer 1, full length 5.8S gene, and partial internal transcribed spacer 2 were determined and compared with available hookworm sequences from public databases. Of the 11 polymerase chain reaction-positive samples, eight (Bago Region, N = 4; Mon State, N = 4) yielded sequences with high similarity to those of Necator americanus A further three sequences (Mon State, N = 2; Bago Region, N = 1) showed high similarity with those of Ancylostoma ceylanicum The latter is primarily a parasite of dogs and represents a zoonosis. Given that different species of hookworms exhibit different epidemiological and biological characteristics, accurate identification is essential for the planning and execution of effective control programs for hookworm infections.
[Mh] Termos MeSH primário: Ancylostoma/isolamento & purificação
Necator americanus/isolamento & purificação
Necatoríase/epidemiologia
Necatoríase/parasitologia
[Mh] Termos MeSH secundário: Animais
DNA de Helmintos/genética
DNA de Helmintos/isolamento & purificação
Fezes/parasitologia
Seres Humanos
Mianmar/epidemiologia
Reação em Cadeia da Polimerase
População Rural
Especificidade da Espécie
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0610


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[PMID]:27977856
[Au] Autor:Elliott DE; Weinstock JV
[Ad] Endereço:Division of Gastroenterology, University of Iowa, Iowa City, IA, USA.
[Ti] Título:Nematodes and human therapeutic trials for inflammatory disease.
[So] Source:Parasite Immunol;39(5), 2017 May.
[Is] ISSN:1365-3024
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Helminth infections likely provide a protective influence against some immune-mediated and metabolic diseases because helminth infection dramatically decreased in developed countries shortly before the explosive rise in the prevalence of these diseases. The capacity of helminths to activate immune-regulatory circuits in their hosts and to modulate the composition of intestinal flora appears to be the mechanisms of protective action. Animal models of disease show that various helminth species prevent and/or block inflammation in various organs in a diverse range of diseases. Clinical trials have demonstrated that medicinal exposure to Trichuris suis or small numbers of Necator americanus is safe with minor, if any, reported adverse effects. This includes exposure of inflamed intestine to T. suis, asthmathic lung to N. americanus and in patients with atopy. Efficacy has been suggested in some small studies, but is absent in others. Factors that may have led to inconclusive results in some trials are discussed. To date, there have been no registered clinical trials using helminths to treat metabolic syndrome or its component conditions. However, the excellent safety profile of T. suis or N. americanus suggests that such studies should be possible.
[Mh] Termos MeSH primário: Helmintíase/imunologia
Inflamação/terapia
Terapia com Helmintos
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Necator americanus/imunologia
Trichuris/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE
[do] DOI:10.1111/pim.12407


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[PMID]:27840196
[Au] Autor:Hasegawa H; Shigyo M; Yanai Y; McLennan MR; Fujita S; Makouloutou P; Tsuchida S; Ando C; Sato H; Huffman MA
[Ad] Endereço:Department of Biology, Faculty of Medicine, Oita University, Hasama, Yufu, Oita 879-5593, Japan. Electronic address: hasegawa@oita-u.ac.jp.
[Ti] Título:Molecular features of hookworm larvae (Necator spp.) raised by coproculture from Ugandan chimpanzees and Gabonese gorillas and humans.
[So] Source:Parasitol Int;66(2):12-15, 2017 Apr.
[Is] ISSN:1873-0329
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Species composition of Necator hookworms was surveyed in (i) Ugandan chimpanzees living around farms and villages at Bulindi, (ii) Gabonese gorillas under habituation in Moukalaba-Doudou National Park (MDNP), and (iii) Gabonese villagers living adjacent to MDNP. Internal transcribed spacers (ITS) of rDNA and partial cytochrome c oxidase subunit 1 (Cox1) gene of mtDNA were analyzed from larvae obtained by coproculture. Three ITS types (I, II and III) and three Cox1 haplotype groups (A, B and C) were demonstrated. ITS type I and Cox1 haplotype group A, representing Necator americanus, were demonstrated in the hookworm larvae from Gabonese gorillas and humans, but not from Ugandan chimpanzees. Type II and haplotype groups B and C, presumably representing N. gorillae, were found in larvae from Ugandan chimpanzees and Gabonese gorillas and humans. These features were overall similar with those found previously in the Central African Republic. Meanwhile, type III was proven in a larva from a Gabonese gorilla as the first demonstration from a non-human primate. Cox1 haplotypes obtained from Ugandan chimpanzees formed a subgroup within group B, presumably reflecting dispersal and diversification processes of the apes.
[Mh] Termos MeSH primário: Fezes/parasitologia
Gorilla gorilla/parasitologia
Necator/genética
Necator/fisiologia
Pan troglodytes/parasitologia
[Mh] Termos MeSH secundário: Animais
Doenças dos Símios Antropoides/parasitologia
Ciclo-Oxigenase 1/genética
Comportamento Alimentar
Gabão
Haplótipos
Seres Humanos
Larva/genética
Larva/crescimento & desenvolvimento
Necator/isolamento & purificação
Necator americanus/genética
Necator americanus/isolamento & purificação
Necator americanus/fisiologia
Necatoríase/parasitologia
Necatoríase/veterinária
Estações do Ano
Análise de Sequência de DNA
Uganda
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.14.99.1 (Cyclooxygenase 1)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170413
[Lr] Data última revisão:
170413
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161115
[St] Status:MEDLINE


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[PMID]:27930648
[Au] Autor:Rashwan N; Bourguinat C; Keller K; Gunawardena NK; de Silva N; Prichard R
[Ad] Endereço:Institute of Parasitology, Macdonald College, McGill University, Ste Anne de Bellevue, QC, Canada.
[Ti] Título:Isothermal Diagnostic Assays for Monitoring Single Nucleotide Polymorphisms in Necator americanus Associated with Benzimidazole Drug Resistance.
[So] Source:PLoS Negl Trop Dis;10(12):e0005113, 2016 Dec.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Soil-transmitted helminths (STHs) are the most prevalent intestinal helminths of humans, and a major cause of morbidity in tropical and subtropical countries. The benzimidazole (BZ) drugs albendazole (ABZ) and mebendazole (MBZ) are used for treatment of human STH infections and this use is increasing dramatically with massive drug donations. Frequent and prolonged use of these drugs could lead to the emergence of anthelmintic resistance as has occurred in nematodes of livestock. Previous molecular assays for putative resistance mutations have been based mainly on PCR amplification and sequencing. However, these techniques are complicated and time consuming and not suitable for resource-constrained situations. A simple, rapid and sensitive genotyping method is required to monitor for possible developing resistance to BZ drugs. METHODS: To address this problem, single nucleotide polymorphism (SNP) detection assays were developed based on the Smart amplification method (SmartAmp2) to target codons 167, 198, and 200 in the ß-tubulin isotype 1 gene for the hookworm Necator americanus. FINDINGS: Diagnostic assays were developed and applied to analyze hookworm samples by both SmartAmp2 and conventional sequencing methods and the results showed high concordance. Additionally, fecal samples spiked with N. americanus larvae were assessed and the results showed that the Aac polymerase used has high tolerance to inhibitors in fecal samples. CONCLUSION: The N. americanus SmartAmp2 SNP detection assay is a new genotyping tool that is rapid, sensitive, highly specific and efficient with the potential to be used as a field tool for monitoring SNPs associated with BZ resistance. However, further validation on large numbers of field samples is required.
[Mh] Termos MeSH primário: Antinematódeos/farmacologia
Benzimidazóis/farmacologia
Resistência a Medicamentos/genética
Necator americanus/genética
Técnicas de Amplificação de Ácido Nucleico/métodos
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Animais
Benzimidazóis/metabolismo
Técnicas de Genotipagem
Seres Humanos
Necator americanus/efeitos dos fármacos
Necator americanus/isolamento & purificação
Sensibilidade e Especificidade
Temperatura Ambiente
Tubulina (Proteína)/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antinematodal Agents); 0 (Benzimidazoles); 0 (Tubulin); E24GX49LD8 (benzimidazole)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161209
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005113


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[PMID]:27363703
[Au] Autor:Keiser J; Panic G; Adelfio R; Cowan N; Vargas M; Scandale I
[Ad] Endereço:Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland. jennifer.keiser@unibas.ch.
[Ti] Título:Evaluation of an FDA approved library against laboratory models of human intestinal nematode infections.
[So] Source:Parasit Vectors;9(1):376, 2016 07 01.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Treatment options for infections with soil-transmitted helminths (STH) - Ascaris lumbricoides, Trichuris trichiura and the two hookworm species, Ancylostoma duodenale and Necator americanus - are limited despite their considerable global health burden. The aim of the present study was to test the activity of an openly available FDA library against laboratory models of human intestinal nematode infections. METHODS: All 1,600 drugs were first screened against Ancylostoma ceylanicum third-stage larvae (L3). Active compounds were scrutinized and toxic compounds, drugs indicated solely for topical use, and already well-studied anthelmintics were excluded. The remaining hit compounds were tested in parallel against Trichuris muris first-stage larvae (L1), Heligmosomoides polygyrus third-stage larvae (L3), and adult stages of the three species in vitro. In vivo studies were performed in the H. polygyrus and T. muris mice models. RESULTS: Fifty-four of the 1,600 compounds tested revealed an activity of > 60 % against A. ceylanicum L3 (hit rate of 3.4 %), following incubation at 200 µM for 72 h. Twelve compounds progressed into further screens. Adult A. ceylanicum were the least affected (1/12 compounds active at 50 µM), while eight of the 12 test compounds revealed activity against T. muris L1 (100 µM) and adults (50 µM), and H. polygyrus L3 (200 µM). Trichlorfon was the only compound active against all stages of A. ceylanicum, H. polygyrus and T. muris. In addition, trichlorfon achieved high worm burden reductions of 80.1 and 98.9 %, following a single oral dose of 200 mg/kg in the T. muris and H. polygyrus mouse model, respectively. CONCLUSION: Drug screening on the larval stages of intestinal parasitic nematodes is feasible using small libraries and important given the empty drug discovery and development pipeline for STH infections. Differences and commonalities in drug activities across the different STH species and stages were confirmed. Hits identified might serve as a starting point for drug discovery for STH.
[Mh] Termos MeSH primário: Anti-Helmínticos/farmacologia
Enteropatias Parasitárias/tratamento farmacológico
Nematoides/efeitos dos fármacos
Infecções por Nematoides/tratamento farmacológico
Bibliotecas de Moléculas Pequenas/normas
[Mh] Termos MeSH secundário: Adulto
Ancylostoma/efeitos dos fármacos
Animais
Anti-Helmínticos/uso terapêutico
Ascaris lumbricoides/efeitos dos fármacos
Cricetinae
Modelos Animais de Doenças
Avaliação Pré-Clínica de Medicamentos
Feminino
Seres Humanos
Masculino
Mesocricetus
Camundongos
Camundongos Endogâmicos C57BL
Necator americanus/efeitos dos fármacos
Bibliotecas de Moléculas Pequenas/farmacologia
Bibliotecas de Moléculas Pequenas/uso terapêutico
Trichuris/efeitos dos fármacos
Estados Unidos
United States Food and Drug Administration
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Small Molecule Libraries)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160702
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-016-1616-0


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[PMID]:27259664
[Au] Autor:Kulkarni AP; Mittal SP
[Ad] Endereço:Bioinformatics Centre, Savitribai Phule Pune University, Pune 411007, Maharashtra, India. Electronic address: abhijeet@bioinfo.net.in.
[Ti] Título:Sequence data mining in search of hookworm (Necator americanus) microRNAs.
[So] Source:Gene;590(2):317-23, 2016 Sep 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The new world hookworm, Necator americanus is a soil-transmitted nematode responsible for Necatoriasis (a type of helminthiasis) in hosts such as humans, dogs, and cats. N. americanus genome and transcriptome has been sequenced and a draft assembly analysis has been published highlighting protein coding genes and possible drug target proteins. Hookworm microRNA identification, annotations and their public release is yet to be attempted. The same is evident from lack of hookworm miRNA information in related popular public nucleotide sequence repositories such as miRBase, GenBank, WormBase etc. Therefore, in the present study we addressed these issues using EST and assembled transcript sequence information of hookworm. Using computational approaches, we identified three miRNAs precursor sequences and their mature forms. We also identified their potential targets from hookworm ESTs and transcripts, and from human transcriptome. Overall, the results indicate presence of nematode specific miRNA homologs in N. americanus and shades light on their putative targets in worm itself and the human host.
[Mh] Termos MeSH primário: Mineração de Dados
MicroRNAs/genética
Necator americanus/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
MicroRNAs/metabolismo
Conformação de Ácido Nucleico
Filogenia
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160605
[St] Status:MEDLINE


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[PMID]:27076158
[Au] Autor:Fuaad AA; Skwarczynski M; Toth I
[Ad] Endereço:School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, St Lucia, QLD, 4072, Australia.
[Ti] Título:The Use of Microwave-Assisted Solid-Phase Peptide Synthesis and Click Chemistry for the Synthesis of Vaccine Candidates Against Hookworm Infection.
[So] Source:Methods Mol Biol;1403:639-53, 2016.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A protein-based vaccine approach against hookworm infection has failed to deliver the expected outcome, due to a problem with an allergic response in the patient or difficulties in the proteins' production. This implication could be overcome by using a chemically synthesized peptide-based vaccine approach. This approach utilizes minimal pathogenic components that are necessary for the stimulation of the immune response without triggering adverse side effects. To boost the peptide's immunogenicity, a lipid core peptide (LCP) system can be utilized as a carrier molecule/immunostimulant. This chapter describes in detail the synthesizing of protected lipoamino acid, the self-adjuvanting moiety (LCP core), the peptide epitope, and the final vaccine candidate. The subunit peptide and the LCP core were synthesized using microwave-assisted solid-phase peptide synthesis (SPPS). Then the final hookworm vaccine construct was assembled using the copper-catalyzed azide-alkyne cycloaddition, or "click," reaction.
[Mh] Termos MeSH primário: Química Click/métodos
Infecções por Uncinaria/prevenção & controle
Micro-Ondas
Técnicas de Síntese em Fase Sólida/métodos
Vacinas/síntese química
[Mh] Termos MeSH secundário: Animais
Epitopos/imunologia
Necator americanus/imunologia
Ácido Palmítico/química
Vacinas/química
Vacinas/imunologia
Vacinas de Subunidades/síntese química
Vacinas de Subunidades/química
Vacinas de Subunidades/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Epitopes); 0 (Vaccines); 0 (Vaccines, Subunit); 2V16EO95H1 (Palmitic Acid)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160415
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-3387-7_36



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