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  1 / 22 MEDLINE  
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[PMID]:28443527
[Au] Autor:McGovern FM; Sweeney T; Ryan MT; Lott S; Campion FP; Boland TM
[Ad] Endereço:1School of Agriculture and Food Science,Lyons Research Farm,University College Dublin (UCD),Newcastle,Co. Kildare,W23 ENY2,Republic of Ireland.
[Ti] Título:An investigation into the effects of maternal supplementation with excess iodine on the mechanisms and impacts of reduced IgG absorption in the lamb postpartum.
[So] Source:Br J Nutr;117(7):951-963, 2017 Apr.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An experiment was conducted to determine: (1) the effect of excess maternal I supplementation on the thyroid hormone status of the ewe and her progeny; (2) potential mechanisms underpinning the failure of passive transfer associated with excess I and (3) the growing lambs' response to natural gastrointestinal infection. Twin-bearing ewes received one of two treatments (n 32/treatment group): basal diet (C) or C plus 26·6 mg of iodine/ewe per d (I), supplied as calcium iodate. Ewes were individually fed from day 119 of gestation to parturition. Progeny of I ewes had lower (P<0·01) serum IgG concentrations from 24 h to 28 d postpartum but higher serum IgG concentrations at day 70 postpartum (P<0·05). I supplementation increased the relative expression of Fc receptor, IgA, IgM high affinity and polymeric Ig receptor in the ileum of the lamb at 24 h postpartum; however, thyroid hormone receptor-ß (THRB) and ß-2-microglobulin (B2M) expression declined (P<0·05). Progeny of I ewes had higher growth rates to weaning (P<0·05) and lower faecal egg count (FEC) for Nematodirus battus (P<0·05) between weeks 6 and 10 postpartum. In conclusion, excess maternal I supplementation negatively affected the thyroid hormone status, serum IgG concentration, ileal morphology and the gene expression of THRB and B2M in the ileum and ras-related protein (RAB) RAB25 and the mucin gene (MUC) MUC1 in the duodenum of the lamb postpartum. These effects were followed by an enhancement of average daily gain and lower N. battus FEC in the pre-weaning period of I-supplemented lambs.
[Mh] Termos MeSH primário: Colostro/imunologia
Suplementos Nutricionais
Imunidade Materno-Adquirida
Iodo/uso terapêutico
Fenômenos Fisiológicos da Nutrição Materna
Doenças dos Ovinos/prevenção & controle
Infecções por Strongylida/veterinária
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Colostro/química
Suplementos Nutricionais/efeitos adversos
Feminino
Regulação da Expressão Gênica no Desenvolvimento
Íleo/crescimento & desenvolvimento
Íleo/imunologia
Íleo/metabolismo
Íleo/patologia
Imunoglobulina G/análise
Imunoglobulina G/biossíntese
Mucosa Intestinal/crescimento & desenvolvimento
Mucosa Intestinal/imunologia
Mucosa Intestinal/metabolismo
Mucosa Intestinal/patologia
Iodo/efeitos adversos
Masculino
Nematodirus/imunologia
Nematodirus/isolamento & purificação
Contagem de Ovos de Parasitas/veterinária
Gravidez
Distribuição Aleatória
Ovinos
Doenças dos Ovinos/imunologia
Doenças dos Ovinos/metabolismo
Doenças dos Ovinos/parasitologia
Carneiro Doméstico
Infecções por Strongylida/imunologia
Infecções por Strongylida/parasitologia
Infecções por Strongylida/prevenção & controle
Receptores beta dos Hormônios Tireóideos/genética
Receptores beta dos Hormônios Tireóideos/metabolismo
Ganho de Peso
Microglobulina-2 beta/genética
Microglobulina-2 beta/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin G); 0 (Thyroid Hormone Receptors beta); 0 (beta 2-Microglobulin); 9679TC07X4 (Iodine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517000575


  2 / 22 MEDLINE  
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[PMID]:27514876
[Au] Autor:Oliver AM; Pomroy WE; Ganesh S; Leathwick DM
[Ad] Endereço:AgResearch Grasslands, Private Bag 11008, Palmerston North 4442, New Zealand.
[Ti] Título:Chilling requirements for hatching of a New Zealand isolate of Nematodirus filicollis.
[So] Source:Vet Parasitol;226:17-21, 2016 Aug 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The eggs of some species of the parasitic nematode Nematodirus require a period of chilling before they can hatch; N. filicollis is one such species. This study investigated this requirement for chilling in a New Zealand strain of this species. Eggs of N. filicollis were extracted from lamb's faeces and incubated at 20°C to allow development to the third stage larvae within the egg. These eggs were then placed into tissue culture plates and incubated at: 2.7°C (±0.99), 3.6°C (±0.90), 4.7°C (±0.35), 6.4°C (±0.37), 8.0°C (±1.54) or 9.9°C (±0.14) for up to 224 days. At 14day intervals until day 84, then every 28 days, one plate was removed from each temperature and placed at 13.1°C (±0.44) for 14 days. Eggs were then assessed for hatching. From this data, chill units were calculated by subtracting the culture temperature from a constant threshold of 11°C and multiplying by the number of days for which the sample was cultured; then the Gompertz model fitted. Even though hatching overall was low, a greater proportion of eggs hatched with chill accumulation. Maximum hatching of eggs required 800-1000 chill units. Consequently in the field, more than one season of chilling would be required before hatching. As such a generation time could take more than one year to complete. This is different to the hatching dynamics of N. spathiger, the other main species found in New Zealand sheep, which does not display this requirement for chilling and hatches immediately once the third stage larvae are developed.
[Mh] Termos MeSH primário: Temperatura Baixa
Nematodirus/crescimento & desenvolvimento
Doenças dos Ovinos/parasitologia
Infecções por Strongylida/veterinária
[Mh] Termos MeSH secundário: Animais
DNA de Helmintos/análise
Fezes/parasitologia
Nematodirus/genética
Nova Zelândia
Óvulo/crescimento & desenvolvimento
Reação em Cadeia da Polimerase
Ovinos
Infecções por Strongylida/parasitologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160813
[St] Status:MEDLINE


  3 / 22 MEDLINE  
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[PMID]:27369575
[Au] Autor:Kenyon F; Rinaldi L; McBean D; Pepe P; Bosco A; Melville L; Devin L; Mitchell G; Ianniello D; Charlier J; Vercruysse J; Cringoli G; Levecke B
[Ad] Endereço:Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK. Electronic address: FionaKenyon@moredun.ac.uk.
[Ti] Título:Pooling sheep faecal samples for the assessment of anthelmintic drug efficacy using McMaster and Mini-FLOTAC in gastrointestinal strongyle and Nematodirus infection.
[So] Source:Vet Parasitol;225:53-60, 2016 Jul 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In small ruminants, faecal egg counts (FECs) and reduction in FECs (FECR) are the most common methods for the assessment of intensity of gastrointestinal (GI) nematodes infections and anthelmintic drug efficacy, respectively. The main limitation of these methods is the time and cost to conduct FECs on a representative number of individual animals. A cost-saving alternative would be to examine pooled faecal samples, however little is known regarding whether pooling can give representative results. In the present study, we compared the FECR results obtained by both an individual and a pooled examination strategy across different pool sizes and analytical sensitivity of the FEC techniques. A survey was conducted on 5 sheep farms in Scotland, where anthelmintic resistance is known to be widespread. Lambs were treated with fenbendazole (4 groups), levamisole (3 groups), ivermectin (3 groups) or moxidectin (1 group). For each group, individual faecal samples were collected from 20 animals, at baseline (D0) and 14 days after (D14) anthelmintic administration. Faecal samples were analyzed as pools of 3-5, 6-10, and 14-20 individual samples. Both individual and pooled samples were screened for GI strongyle and Nematodirus eggs using two FEC techniques with three different levels of analytical sensitivity, including Mini-FLOTAC (analytical sensitivity of 10 eggs per gram of faeces (EPG)) and McMaster (analytical sensitivity of 15 or 50 EPG).For both Mini-FLOTAC and McMaster (analytical sensitivity of 15 EPG), there was a perfect agreement in classifying the efficacy of the anthelmintic as 'normal', 'doubtful' or 'reduced' regardless of pool size. When using the McMaster method (analytical sensitivity of 50 EPG) anthelmintic efficacy was often falsely classified as 'normal' or assessment was not possible due to zero FECs at D0, and this became more pronounced when the pool size increased. In conclusion, pooling ovine faecal samples holds promise as a cost-saving and efficient strategy for assessing GI nematode FECR. However, for the assessment FECR one will need to consider the baseline FEC, pool size and analytical sensitivity of the method.
[Mh] Termos MeSH primário: Anti-Helmínticos/farmacologia
Fezes/parasitologia
Gastroenteropatias/veterinária
Nematoides/efeitos dos fármacos
Parasitologia/métodos
Doenças dos Ovinos
Infecções por Strongylida/veterinária
[Mh] Termos MeSH secundário: Animais
Anti-Helmínticos/uso terapêutico
Gastroenteropatias/tratamento farmacológico
Gastroenteropatias/parasitologia
Nematodirus/efeitos dos fármacos
Contagem de Ovos de Parasitas
Ovinos
Doenças dos Ovinos/tratamento farmacológico
Doenças dos Ovinos/parasitologia
Infecções por Strongylida/diagnóstico
Infecções por Strongylida/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160703
[St] Status:MEDLINE


  4 / 22 MEDLINE  
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[PMID]:26966307
[Au] Autor:Mitchell S; Hopkins B; Corfield C
[Ad] Endereço:APHA Carmarthen Veterinary Investigation Centre, Jobs Well Road, Johnstown, Carmarthen SA32 3EZ, e-mail: Sian.Mitchell@apha.gsi.gov.uk.
[Ti] Título:Nematodirus lamae identified in an alpaca in the UK.
[So] Source:Vet Rec;178(11):271-2, 2016 Mar 12.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Camelídeos Americanos/parasitologia
Nematodirus/isolamento & purificação
Doenças Parasitárias em Animais/diagnóstico
[Mh] Termos MeSH secundário: Animais
Autopsia/veterinária
Fezes/parasitologia
Reino Unido
[Pt] Tipo de publicação:CASE REPORTS; LETTER
[Em] Mês de entrada:1607
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160312
[St] Status:MEDLINE
[do] DOI:10.1136/vr.i1411


  5 / 22 MEDLINE  
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[PMID]:26846152
[Au] Autor:Oliver A; Pomroy WE; Leathwick DM
[Ad] Endereço:a Animal Health, AgResearch Grasslands , Private Bag 11008, Palmerston North 4442 , New Zealand.
[Ti] Título:Benzimidazole resistance in Nematodirus spathiger and N. filicollis in New Zealand.
[So] Source:N Z Vet J;64(4):201-6, 2016 Jul.
[Is] ISSN:1176-0710
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: To determine the prevalence of benzimidazole resistance in Nematodirus spathiger and N. filicollis from a sample of New Zealand farms. METHODS: The efficacy of albendazole (ABZ) against Nematodirus spp. was assessed by faecal nematode egg count reduction (FECR) tests undertaken in lambs aged 3-8 months old on 27 sheep farms throughout New Zealand. On each farm, groups of 10-16 lambs were either treated with ABZ (4.75 mg/kg) or remained as untreated controls. Faecal samples were collected from all animals at the time of treatment and 7-10 days later. Faecal nematode egg counts (FEC) were performed using a modified McMaster technique. Larvae were cultured from pooled faecal samples, collected 7-10 days after treatment from each group, by incubation at 20°C for 6 weeks, 4°C for 26 weeks then 13°C for 2 weeks. The resulting third stage larvae were identified to species using a multiplex PCR assay, that identified species-specific sequences in the second internal transcribed spacer region of ribosomal DNA. The efficacy of ABZ for N. spathiger and N. filicollis was calculated from the proportion of the two species in culture and the group mean FEC before and after treatment. Only farms with a mean of 10 epg for each species in untreated samples were included for analysis. Resistance was defined as an efficacy <95%. RESULTS: On farms that met the threshold of 10 epg in faecal samples, benzimidazole resistance was found on 20/21 (95%) farms for N. spathiger compared with 4/10 (40%) farms for N. filicollis (p<0.05). In samples collected following treatment, a mean of 83 (min 46, max 100)% of Nematodirus spp. larvae recovered from the untreated groups were N. spathiger, compared with 94 (min 45, max 100)% in the ABZ treated groups (p=0.03). This change in percentage was not influenced by the overall efficacy of treatment based on the FECR test (p=0.324). CONCLUSION: The results confirm the high level of resistance in N. spathiger in New Zealand and that benzimidazole resistance was more common in N. spathiger than N. filicollis. While resistance to benzimidazole anthelmintics has been reported previously in New Zealand, this is the first report of N. filicollis being resistant to benzimidazole anthelmintics.
[Mh] Termos MeSH primário: Albendazol/farmacologia
Resistência a Medicamentos
Nematodirus/efeitos dos fármacos
Doenças dos Ovinos/parasitologia
Infecções por Strongylida/veterinária
[Mh] Termos MeSH secundário: Animais
Anti-Helmínticos/farmacologia
Fezes/parasitologia
Nova Zelândia/epidemiologia
Contagem de Ovos de Parasitas
Ovinos
Doenças dos Ovinos/epidemiologia
Infecções por Strongylida/epidemiologia
Infecções por Strongylida/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); F4216019LN (Albendazole)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170203
[Lr] Data última revisão:
170203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160206
[St] Status:MEDLINE
[do] DOI:10.1080/00480169.2016.1149117


  6 / 22 MEDLINE  
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[PMID]:26795855
[Ti] Título:Finding new ways to prevent disease in food-producing animals.
[So] Source:Vet Rec;178(4):84, 2016 Jan 23.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Increasing concern about antimicrobial resistance and moves to restrict the use of antibiotics in food-producing animals mean that farmers will need new ways of preventing and controlling disease in their animals. With its focus on addressing the needs of the farming industry, the Moredun Research Institute sees this as an opportunity to be at the forefront of developing new solutions. Kristy Ebanks reports from an event organised to showcase some of the institute's latest research.
[Mh] Termos MeSH primário: Agricultura/métodos
Doenças dos Ovinos/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Benzimidazóis/farmacologia
Congressos como Assunto
Resistência a Medicamentos
Seres Humanos
Nanomedicina
Nematodirus/efeitos dos fármacos
Adenomatose Pulmonar Ovina/diagnóstico por imagem
Adenomatose Pulmonar Ovina/prevenção & controle
Ovinos
Tuberculose/terapia
Ultrassonografia
Reino Unido
[Pt] Tipo de publicação:NEWS
[Nm] Nome de substância:
0 (Benzimidazoles); E24GX49LD8 (benzimidazole)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160123
[St] Status:MEDLINE
[do] DOI:10.1136/vr.i342


  7 / 22 MEDLINE  
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[PMID]:26091257
[Au] Autor:Gethings OJ; Rose H; Mitchell S; Van Dijk J; Morgan ER
[Ti] Título:Asynchrony in host and parasite phenology may decrease disease risk in livestock under climate warming: Nematodirus battus in lambs as a case study.
[So] Source:Parasitology;142(10):1306-17, 2015 Sep.
[Is] ISSN:1469-8161
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mismatch in the phenology of trophically linked species as a result of climate warming has been shown to have far-reaching effects on animal communities, but implications for disease have so far received limited attention. This paper presents evidence suggestive of phenological asynchrony in a host-parasite system arising from climate change, with impacts on transmission. Diagnostic laboratory data on outbreaks of infection with the pathogenic nematode Nematodirus battus in sheep flocks in the UK were used to validate region-specific models of the effect of spring temperature on parasite transmission. The hatching of parasite eggs to produce infective larvae is driven by temperature, while the availability of susceptible hosts depends on lambing date, which is relatively insensitive to inter-annual variation in spring temperature. In southern areas and in warmer years, earlier emergence of infective larvae in spring was predicted, with decline through mortality before peak availability of susceptible lambs. Data confirmed model predictions, with fewer outbreaks recorded in those years and regions. Overlap between larval peaks and lamb availability was not reduced in northern areas, which experienced no decreases in the number of reported outbreaks. Results suggest that phenological asynchrony arising from climate warming may affect parasite transmission, with non-linear but predictable impacts on disease burden. Improved understanding of complex responses of host-parasite systems to climate change can contribute to effective adaptation of parasite control strategies.
[Mh] Termos MeSH primário: Mudança Climática
Interações Hospedeiro-Parasita/fisiologia
Nematodirus/fisiologia
Doenças dos Ovinos/transmissão
Infecções por Strongylida/veterinária
[Mh] Termos MeSH secundário: Animais
Temperatura Alta
Modelos Biológicos
Ovinos
Doenças dos Ovinos/epidemiologia
Infecções por Strongylida/epidemiologia
Infecções por Strongylida/transmissão
Fatores de Tempo
Reino Unido/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1512
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150620
[St] Status:MEDLINE


  8 / 22 MEDLINE  
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[PMID]:24331581
[Au] Autor:Zhao GH; Jia YQ; Bian QQ; Nisbet AJ; Cheng WY; Liu Y; Fang YQ; Ma XT; Yu SK
[Ad] Endereço:College of Veterinary Medicine, Northwest A&F University,Yangling712100,Shaanxi Province,PR China.
[Ti] Título:Molecular approaches to differentiate three species of Nematodirus in sheep and goats from China based on internal transcribed spacer rDNA sequences.
[So] Source:J Helminthol;89(3):259-66, 2015 May.
[Is] ISSN:1475-2697
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Internal transcribed spacer (ITS) rDNA sequences of three Nematodirus species from naturally infected goats or sheep in two endemic provinces of China were analysed to establish an effective molecular approach to differentiate Nematodirus species in small ruminants. The respective intra-specific genetic variations in ITS1 and ITS2 rDNA regions were 0.3-1.8% and 0-0.4% in N. spathiger, 0-6.5% and 0-5.4% in N. helvetianus, and 0-4.4% and 0-6.1% in N. oiratianus from China. The respective intra-specific variations of ITS1 and ITS2 were 1.8-4.4% and 1.6-6.1% between N. oiratianus isolates from China and Iran, 5.7-7.1% and 6.3-8.3% between N. helvetianus samples from China and America. For N. spathiger, compared with samples from China, sequence differences in ITS1 rDNA were 0.3-2.4% in isolates from America, 0.3-2.9% in New Zealand and 2.1-2.4% in Australia. Genetic variations in ITS2 rDNA of N. spathiger were 0-0.4% between samples from China and America, and 0-0.8% between samples from China and New Zealand. Using mutation sites, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and specific PCR techniques were developed to differentiate these three Nematodirus species. The specific PCR assay allowed the accurate identification of N. oiratianus from other common nematodes with a sensitivity of 0.69 pg and further examination of Nematodirus samples demonstrated the reliability of these two molecular methods.
[Mh] Termos MeSH primário: Variação Genética
Doenças das Cabras/parasitologia
Técnicas de Diagnóstico Molecular/métodos
Nematodirus/classificação
Nematodirus/genética
Doenças dos Ovinos/parasitologia
Infecções por Strongylida/veterinária
[Mh] Termos MeSH secundário: Animais
China
Análise por Conglomerados
Primers do DNA/genética
DNA de Helmintos/química
DNA de Helmintos/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Cabras
Dados de Sequência Molecular
Nematodirus/isolamento & purificação
Filogenia
Reação em Cadeia da Polimerase/métodos
Polimorfismo de Fragmento de Restrição
Sensibilidade e Especificidade
Análise de Sequência de DNA
Ovinos
Infecções por Strongylida/parasitologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Helminth); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150331
[Lr] Data última revisão:
150331
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131217
[St] Status:MEDLINE
[do] DOI:10.1017/S0022149X13000825


  9 / 22 MEDLINE  
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[PMID]:25487605
[Au] Autor:Morrison AA; Mitchell S; Mearns R; Richards I; Matthews JB; Bartley DJ
[Ad] Endereço:Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, UK. alison.morrison@moredun.ac.uk.
[Ti] Título:Phenotypic and genotypic analysis of benzimidazole resistance in the ovine parasite Nematodirus battus.
[So] Source:Vet Res;45:116, 2014 Dec 09.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Benzimidazole resistance is common amongst many ovine trichostrongylid nematodes species globally. Although anthelmintics have been used for over half a century in some areas of the world for the control of Nematodirus battus, resistance has never been detected. Veterinary investigations conducted in 2010 demonstrated reduced efficacy in a flock that had been treated previously with fenbendazole (FBZ), suggesting probable resistance in N. battus. Infective larvae (L3; designated MNba2) were generated from the original material to conduct a controlled efficacy test (CET). Faecal egg counts showed an average of 37% reduction in the FBZ treated group 7 days post treatment compared to the untreated lambs. Average worm burden results showed no reduction after FBZ treatment compared to the untreated group (3850 and 3850 worms respectively). A molecular assay to assess the frequency of the commonly associated single nucleotide polymorphisms (SNP) in the ß-tubulin isotype 1 gene, F200Y and E198A, was developed. Larval genotypes were predominantly homozygous resistant at codon 200 SNP, ranging from 56%-83% and remained stable at 70% for adult worm populations taken from treated and control lambs in the CET. Only susceptible genotypes were found at codon 198. The allele frequency for F200Y ranged between 80-83% in adult worms taken from the CET from treated and control lambs. The results confirmed initial findings and demonstrated the first report of FBZ resistance in N. battus whilst providing evidence that the P200 point mutation in the ß-tubulin isotype 1 gene is a potential mechanism of resistance in the species.
[Mh] Termos MeSH primário: Benzimidazóis/farmacologia
Resistência a Medicamentos/genética
Proteínas de Helminto/genética
Nematodirus/efeitos dos fármacos
Nematodirus/genética
Contagem de Ovos de Parasitas/veterinária
Tubulina (Proteína)/genética
[Mh] Termos MeSH secundário: Animais
Anti-Helmínticos/farmacologia
Fezes/parasitologia
Genótipo
Proteínas de Helminto/metabolismo
Larva/efeitos dos fármacos
Reação em Cadeia da Polimerase/veterinária
Polimorfismo de Nucleotídeo Único
Ovinos
Doenças dos Ovinos/parasitologia
Doenças dos Ovinos/prevenção & controle
Infecções por Strongylida/parasitologia
Infecções por Strongylida/prevenção & controle
Infecções por Strongylida/veterinária
Tubulina (Proteína)/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Benzimidazoles); 0 (Helminth Proteins); 0 (Tubulin); E24GX49LD8 (benzimidazole)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141210
[St] Status:MEDLINE
[do] DOI:10.1186/s13567-014-0116-5


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[PMID]:25015379
[Au] Autor:Zhao GH; Jia YQ; Cheng WY; Zhao W; Bian QQ; Liu GH
[Ad] Endereço:College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, People's Republic of China. zgh083@nwsuaf.edu.cn.
[Ti] Título:Characterization of the complete mitochondrial genomes of Nematodirus oiratianus and Nematodirus spathiger of small ruminants.
[So] Source:Parasit Vectors;7:319, 2014 Jul 11.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Nematodirus spp. are among the most common nematodes of ruminants worldwide. N. oiratianus and N. spathiger are distributed worldwide as highly prevalent gastrointestinal nematodes, which cause emerging health problems and economic losses. Accurate identification of Nematodirus species is essential to develop effective control strategies for Nematodirus infection in ruminants. Mitochondrial DNA (mtDNA) could provide powerful genetic markers for identifying these closely related species and resolving phylogenetic relationships at different taxonomic levels. METHODS: In the present study, the complete mitochondrial (mt) genomes of N. oiratianus and N. spathiger from small ruminants in China were obtained using Long-range PCR and sequencing. RESULTS: The complete mt genomes of N. oiratianus and N. spathiger were 13,765 bp and 13,519 bp in length, respectively. Both mt genomes were circular and consisted of 36 genes, including 12 genes encoding proteins, 2 genes encoding rRNA, and 22 genes encoding tRNA. Phylogenetic analyses based on the concatenated amino acid sequence data of all 12 protein-coding genes by Bayesian inference (BI), Maximum likelihood (ML) and Maximum parsimony (MP) showed that the two Nematodirus species (Molineidae) were closely related to Dictyocaulidae. CONCLUSIONS: The availability of the complete mtDNA sequences of N. oiratianus and N. spathiger not only provides new mtDNA sources for a better understanding of nematode mt genomics and phylogeny, but also provides novel and useful genetic markers for studying diagnosis, population genetics and molecular epidemiology of Nematodirus spp. in small ruminants.
[Mh] Termos MeSH primário: Genoma Mitocondrial
Doenças das Cabras/parasitologia
Nematodirus/genética
Doenças dos Ovinos/parasitologia
Infecções por Strongylida/veterinária
[Mh] Termos MeSH secundário: Animais
China/epidemiologia
Cabras
Filogenia
Ovinos
Infecções por Strongylida/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1503
[Cu] Atualização por classe:150805
[Lr] Data última revisão:
150805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140713
[St] Status:MEDLINE
[do] DOI:10.1186/1756-3305-7-319



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