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[PMID]:28265572
[Au] Autor:Seyoum Z; Zewdu A; Dagnachew S; Bogale B
[Ad] Endereço:Department of Paraclinical Studies, Faculty of Veterinary Medicine, University of Gondar, Gondar, Ethiopia.
[Ti] Título:Anthelmintic Resistance of Strongyle Nematodes to Ivermectin and Fenbendazole on Cart Horses in Gondar, Northwest Ethiopia.
[So] Source:Biomed Res Int;2017:5163968, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A study was conducted from November 2015 to April 2016 to determine fenbendazole and ivermectin resistance status of intestinal nematodes of cart horses in Gondar, Northwest Ethiopia. Forty-five strongyle infected animals were used for this study. The animals were randomly allocated into three groups (15 horses per group). Group I was treated with fenbendazole and Group II with ivermectin and Group III was left untreated. Faecal samples were collected from each cart horse before and after treatment. Accordingly, the reduction in the mean fecal egg count at fourteen days of treatment for ivermectin and fenbendazole was 97.25% and 79.4%, respectively. It was significantly different in net egg count between treatment and control groups after treatment. From the study, resistance level was determined for fenbendazole and suspected for ivermectin. In addition, a questionnaire survey was also conducted on 90 selected cart owners to assess their perception on anthelmintics. In the survey, the most available drugs in the study area used by the owners were fenbendazole and ivermectin. Most respondents have no knowledge about drug management techniques. Hence, animal health extension services to create awareness regarding anthelmintic management that plays a key role in reducing the anthelmintic resistance parasites.
[Mh] Termos MeSH primário: Resistência a Medicamentos
Cavalos/parasitologia
Ivermectina/farmacologia
Infecções Equinas por Strongyloidea/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Etiópia/epidemiologia
Infecções Equinas por Strongyloidea/epidemiologia
Strongylus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
70288-86-7 (Ivermectin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE
[do] DOI:10.1155/2017/5163968


  2 / 167 MEDLINE  
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[PMID]:28249767
[Au] Autor:Tzelos T; Barbeito JS; Nielsen MK; Morgan ER; Hodgkinson JE; Matthews JB
[Ad] Endereço:Moredun Research Institute, Pentlands Science Park, Edinburgh, EH26 0PZ, UK. Electronic address: thomas.tzelos@moredun.ac.uk.
[Ti] Título:Strongyle egg reappearance period after moxidectin treatment and its relationship with management factors in UK equine populations.
[So] Source:Vet Parasitol;237:70-76, 2017 Apr 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Parasitic nematodes, particularly cyathostomins, are ubiquitous in grazing horses world-wide. Considerable burdens of cyathostomin larvae can encyst in the large intestinal wall. The most recommended treatment against these pathogenic stages is moxidectin. Information is required on how effective moxidectin is against cyathostomin populations in different regions. The objectives here were to determine the efficacy of moxidectin treatment and estimate the strongyle egg reappearance period (ERP) after treatment in several equine populations, to confirm the type of strongyle nematodes present and to identify other (i.e. management) factors associated with shortened ERP. Eight yards were recruited and moxidectin in combination with praziquantel administered to all horses (n=261). Faecal egg count (FEC) analysis was performed at weeks 0, 2, 6, 10 and 12 after treatment to determine efficacy and ERP. The ERP was estimated using two previously published methods. Morphological identification of cultured third stage larvae from the sample population was compared to a Strongylus vulgaris-specific end-point PCR to examine the presence of S. vulgaris in samples before and after treatment. Strongyle egg shedding patterns were also compared to worm management practices at each site. At 2 weeks post-treatment, moxidectin was highly effective (faecal egg count reduction range, 99.9-100%). The strongyle ERP ranged from 6 weeks to >12 weeks depending on the calculation method applied. Only cyathostomin larvae were detected by morphological identification. The results from the coprocultures and PCR showed that S. vulgaris was absent before and after treatment. Analysis revealed that regular faecal removal from pasture was associated with lower average FEC and lower prevalence of egg shedding.
[Mh] Termos MeSH primário: Anti-Helmínticos/uso terapêutico
Macrolídeos/uso terapêutico
Praziquantel/uso terapêutico
Infecções Equinas por Strongyloidea/epidemiologia
Strongylus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Fezes/parasitologia
Feminino
Cavalos
Larva
Contagem de Ovos de Parasitas/veterinária
Infecções Equinas por Strongyloidea/tratamento farmacológico
Infecções Equinas por Strongyloidea/parasitologia
Strongylus/fisiologia
Resultado do Tratamento
Reino Unido/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Macrolides); 51570-36-6 (milbemycin); 6490C9U457 (Praziquantel)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE


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[PMID]:28077153
[Au] Autor:Kaspar A; Pfister K; Nielsen MK; Silaghi C; Fink H; Scheuerle MC
[Ad] Endereço:Comparative Tropical Medicine and Parasitology, Veterinary Faculty, Ludwig-Maximilians-University, Munich, Germany. alexandra.kaspar@tropa.vetmed.uni-muenchen.de.
[Ti] Título:Detection of Strongylus vulgaris in equine faecal samples by real-time PCR and larval culture - method comparison and occurrence assessment.
[So] Source:BMC Vet Res;13(1):19, 2017 Jan 11.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Strongylus vulgaris has become a rare parasite in Germany during the past 50 years due to the practice of frequent prophylactic anthelmintic therapy. To date, the emerging development of resistance in Cyathostominae and Parascaris spp. to numerous equine anthelmintics has changed deworming management and the frequency of anthelmintic usage. In this regard, reliable detection of parasitic infections, especially of the highly pathogenic S. vulgaris is essential. In the current study, two diagnostic methods for the detection of infections with S. vulgaris were compared and information on the occurrence of this parasite in German horses was gained. For this purpose, faecal samples of 501 horses were screened for S. vulgaris with real-time PCR and an additional larval culture was performed in samples of 278 horses. A subset of 26 horses underwent multiple follow-up examinations with both methods in order to evaluate both the persistence of S. vulgaris infections and the reproducibility of each diagnostic method. RESULTS: The real-time PCR revealed S. vulgaris-DNA in ten of 501 investigated equine samples (1.9%). The larval culture demonstrated larvae of S. vulgaris in three of the 278 samples (1.1%). A direct comparison of the two methods was possible in 321 samples including 43 follow-up examinations with the result of 11 S. vulgaris-positive samples by real-time PCR and 4 S. vulgaris-positive samples by larval culture. The McNemar's test (p-value = 0.016) revealed a significant difference and the kappa values (0.525) showed a moderate agreement between real-time PCR and larval culture. CONCLUSIONS: The real-time PCR detected a significantly higher proportion of positives of S. vulgaris compared to larval culture and should thus be considered as a routine diagnostic method for the detection of S. vulgaris in equine samples.
[Mh] Termos MeSH primário: Fezes/parasitologia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Infecções Equinas por Strongyloidea/parasitologia
Strongylus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Cavalos
Larva/fisiologia
Contagem de Ovos de Parasitas/veterinária
Reação em Cadeia da Polimerase em Tempo Real/métodos
Infecções Equinas por Strongyloidea/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-016-0918-y


  4 / 167 MEDLINE  
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[PMID]:27302747
[Au] Autor:Whittaker JH; Carlson SA; Jones DE; Brewer MT
[Ad] Endereço:Department of Biomedical Sciences, Iowa State University College of Veterinary Medicine, Ames, IA, USA.
[Ti] Título:Molecular mechanisms for anthelmintic resistance in strongyle nematode parasites of veterinary importance.
[So] Source:J Vet Pharmacol Ther;40(2):105-115, 2017 Apr.
[Is] ISSN:1365-2885
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Veterinarians rely on a relatively limited spectrum of anthelmintic agents to control nematode parasites in domestic animals. Unfortunately, anthelmintic resistance has been an emerging problem in veterinary medicine. In particular, resistance has emerged among the strongyles, a group of gastrointestinal nematodes that infect a variety of hosts that range from large herbivores to small companion animals. Over the last several decades, a great deal of research effort has been directed toward developing an understanding of the mechanisms conferring resistance against the three major groups of anthelmintics: macrocyclic lactones, benzimidazoles, and nicotinic agonists. Our understanding of anthelmintic resistance has been largely formed by determining the mechanism of action for each drug class and then evaluating drug-resistant nematode isolates for mutations or differences in expression of target genes. More recently, drug efflux pumps have been recognized for their potential contribution to anthelmintic resistance. In this mini-review, we summarize the evidence for mechanisms of resistance in strongyle nematodes.
[Mh] Termos MeSH primário: Anti-Helmínticos/farmacologia
Resistência a Medicamentos
Strongylus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Anti-Helmínticos/classificação
Strongylus/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anthelmintics)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160616
[St] Status:MEDLINE
[do] DOI:10.1111/jvp.12330


  5 / 167 MEDLINE  
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[PMID]:27884444
[Au] Autor:Fabiani JV; Lyons ET; Nielsen MK
[Ad] Endereço:M.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA.
[Ti] Título:Dynamics of Parascaris and Strongylus spp. parasites in untreated juvenile horses.
[So] Source:Vet Parasitol;230:62-66, 2016 Oct 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Parasite control in foals is of utmost importance due to the high susceptibility to parasitic infection and disease in this age group. Foals are commonly co-infected with strongyle and ascarid parasites, which complicate parasite control strategies. The present study retrospectively investigated necropsy records of foals born into a university herd kept without anthelmintic treatment since 1979. The aims were to statistically analyze the relationship between fecal egg counts, worm burdens, foal age, sex, and season with specific focus on Parascaris and Strongylus spp. A total of 83 foals born between 1999 and 2015 were included. Foals were born between January and September within the given year and age at necropsy ranged between 27 and 563 days of age with a mean and median of 202 and 204 days, respectively. One set of multivariate mixed linear models was constructed analyzing strongyle and ascarid fecal egg counts as outcome variables, and another set of analyses investigated the following worm counts as outcome variables: Intestinal Parascaris spp. counts (immatures and adults), S. vulgaris (migrating and intestinal stages), S. edentatus (migrating and intestinal stages). Both ascarid and strongyle egg counts were influenced significantly by differences between study years (p<0.05). In addition, total ascarid egg counts were statistically influenced by age (p=0.020) exhibiting a peak at four months of age and fillies had significantly higher ascarid worm burdens (p=0.043). Foal age had significant influences on intestinal counts of immature Parascaris spp. (p=0.034) and adult S. edentatus counts (p=0.028). Larval counts of S. edentatus were significantly associated with birth month (p=0.023), whereas counts of migrating S. vulgaris larvae were not statistically associated with any of the investigated covariates. This study provides novel information on the dynamics of important parasites in naturally infected foals.
[Mh] Termos MeSH primário: Infecções por Ascaridida/veterinária
Doenças dos Cavalos/parasitologia
Infecções por Strongylida/veterinária
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Infecções por Ascaridida/parasitologia
Infecções por Ascaridida/prevenção & controle
Ascaridoidea
Fezes/parasitologia
Feminino
Doenças dos Cavalos/prevenção & controle
Cavalos
Interações Hospedeiro-Parasita
Intestinos/parasitologia
Masculino
Contagem de Ovos de Parasitas
Estudos Retrospectivos
Estações do Ano
Fatores Sexuais
Infecções por Strongylida/parasitologia
Infecções por Strongylida/prevenção & controle
Strongylus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE


  6 / 167 MEDLINE  
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[PMID]:27046508
[Au] Autor:Debeffe L; McLoughlin PD; Medill SA; Stewart K; Andres D; Shury T; Wagner B; Jenkins E; Gilleard JS; Poissant J
[Ad] Endereço:Department of Biology,University of Saskatchewan,112 Science Place, Saskatoon, SK S7N 5E2,Canada.
[Ti] Título:Negative covariance between parasite load and body condition in a population of feral horses.
[So] Source:Parasitology;143(8):983-97, 2016 07.
[Is] ISSN:1469-8161
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In wild and domestic animals, gastrointestinal parasites can have significant impacts on host development, condition, health, reproduction and longevity. Improving our understanding of the causes and consequences of individual-level variation in parasite load is therefore of prime interest. Here we investigated the relationship between strongyle fecal egg count (FEC) and body condition in a unique, naturalized population of horses that has never been exposed to anthelmintic drugs (Sable Island, Nova Scotia, Canada). We first quantified variation in FEC and condition for 447 individuals according to intrinsic (sex, age, reproductive status, social status) and extrinsic (group size, location, local density) variables. We then quantified the repeatability of measurements obtained over a field season and tested for covariance between FEC and condition. FECs were high relative to other horse populations (mean eggs per gram ± SD = 1543·28 ± 209·94). FECs generally decreased with age, were higher in lactating vs non-lactating females, and unexpectedly lower in males in some part of the island. FECs and condition were both spatially structured, with patterns depending on age, sex and reproductive status. FECs and condition were both repeatable. Most notably, FECs and condition were negatively correlated, especially in adult females.
[Mh] Termos MeSH primário: Doenças dos Cavalos/parasitologia
Cavalos/parasitologia
Interações Hospedeiro-Parasita
Infecções Equinas por Strongyloidea/parasitologia
Strongylus/isolamento & purificação
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Canadá
Fezes/parasitologia
Feminino
Masculino
Contagem de Ovos de Parasitas/veterinária
Carga Parasitária
Estações do Ano
Fatores Sexuais
Strongylus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170412
[Lr] Data última revisão:
170412
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160406
[St] Status:MEDLINE
[do] DOI:10.1017/S0031182016000408


  7 / 167 MEDLINE  
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[PMID]:25604521
[Au] Autor:Nielsen MK; Jacobsen S; Olsen SN; Bousquet E; Pihl T
[Ad] Endereço:Department of Veterinary Science, M.H. Gluck Equine Research Center, University of Kentucky, Lexington, USA.
[Ti] Título:Nonstrangulating intestinal infarction associated with Strongylus vulgaris in referred Danish equine cases.
[So] Source:Equine Vet J;48(3):376-9, 2016 May.
[Is] ISSN:2042-3306
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:REASONS FOR PERFORMING STUDY: Strongylus vulgaris is a pathogenic helminth parasite infecting horses and was once considered to be the primary cause of colic. Migrating larvae cause ischaemia and infarction of intestinal segments. This knowledge is derived from case reports and experimental inoculations of parasite-naïve foals, and it remains unknown to what extent the parasite is associated with different types of colic. OBJECTIVES: To evaluate the role of S. vulgaris as a risk factor for different types of colic in horses. STUDY DESIGN: A retrospective case-control study among horses referred with abdominal pain to the University of Copenhagen Large Animal Teaching Hospital during 2009-2011. METHODS: Each colic case was matched with an equid of the same type (pony, Warmblooded or Coldblooded), age, sex and admitted in the same month and year but for problems unrelated to the gastrointestinal tract. Serum samples were analysed for antibodies to migrating S. vulgaris larvae using a recently developed enzyme-linked immunosorbent assay (ELISA). The following 4 case definitions were used: colic sensu lato, i.e. all horses presenting with colic (n = 274), with further subgroups, i.e. undiagnosed colics (n = 48), strangulating obstructions (n = 76) and nonstrangulating infarctions (n = 20). RESULTS: Strongylus vulgaris antibody levels were similar to control values in colics sensu lato and horses with undiagnosed colic. In contrast, nonstrangulating intestinal infarctions were significantly associated with positive S. vulgaris ELISAs (odds ratio 5.33, 95% confidence interval 1.03-27.76, P = 0.05). Also, horses with nonstrangulating infarctions had a significantly higher occurrence of positive ELISAs than horses with strangulating obstructions (odds ratio 3.79, 95% confidence interval 1.34-10.68, P = 0.01) and the colic sensu lato group (odds ratio 3.09, 95% confidence interval 1.20-8.01, P = 0.02). CONCLUSIONS: Nonstrangulating intestinal infarction was strongly associated with S. vulgaris-specific antibodies, whereas the more broadly defined colic categories were not associated with positive ELISA results. Thus, the ELISA holds potential to become a helpful adjunct in diagnosis and management of horses with colic.
[Mh] Termos MeSH primário: Doenças dos Cavalos/parasitologia
Infarto/veterinária
Enteropatias Parasitárias/veterinária
Infecções por Strongylida/veterinária
Strongylus/classificação
[Mh] Termos MeSH secundário: Animais
Cólica/etiologia
Cólica/patologia
Cólica/veterinária
Dinamarca/epidemiologia
Doenças dos Cavalos/epidemiologia
Doenças dos Cavalos/patologia
Cavalos
Infarto/epidemiologia
Infarto/parasitologia
Infarto/patologia
Enteropatias Parasitárias/epidemiologia
Enteropatias Parasitárias/patologia
Infecções por Strongylida/complicações
Infecções por Strongylida/epidemiologia
Infecções por Strongylida/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150122
[St] Status:MEDLINE
[do] DOI:10.1111/evj.12422


  8 / 167 MEDLINE  
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[PMID]:26366671
[Au] Autor:Xu WW; Qiu JH; Liu GH; Zhang Y; Liu ZX; Duan H; Yue DM; Chang QC; Wang CR; Zhao XC
[Ad] Endereço:College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
[Ti] Título:The complete mitochondrial genome of Strongylus equinus (Chromadorea: Strongylidae): Comparison with other closely related species and phylogenetic analyses.
[So] Source:Exp Parasitol;159:94-9, 2015 Dec.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The roundworms of genus Strongylus are the common parasitic nematodes in the large intestine of equine, causing significant economic losses to the livestock industries. In spite of its importance, the genetic data and epidemiology of this parasite are not entirely understood. In the present study, the complete S. equinus mitochondrial (mt) genome was determined. The length of S. equinus mt genome DNA sequence is 14,545 bp, containing 36 genes, of which 12 code for protein, 22 for transfer RNA, and two for ribosomal RNA, but lacks atp8 gene. All 36 genes are encoded in the same direction which is consistent with all other Chromadorea nematode mtDNAs published to date. Phylogenetic analysis based on concatenated amino acid sequence data of all 12 protein-coding genes showed that there were two large branches in the Strongyloidea nematodes, and S. equinus is genetically closer to S. vulgaris than to Cylicocyclus insignis in Strongylidae. This new mt genome provides a source of genetic markers for the molecular phylogeny and population genetics of equine strongyles.
[Mh] Termos MeSH primário: DNA de Helmintos/química
DNA Mitocondrial/química
Genoma Mitocondrial/genética
Filogenia
Strongylus/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Complexo IV da Cadeia de Transporte de Elétrons/genética
Equidae/parasitologia
Cavalos
Intestino Grosso/parasitologia
RNA de Transferência/genética
Infecções Equinas por Strongyloidea/parasitologia
Strongylus/classificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Helminth); 0 (DNA, Mitochondrial); 9014-25-9 (RNA, Transfer); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:151215
[Lr] Data última revisão:
151215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150915
[St] Status:MEDLINE


  9 / 167 MEDLINE  
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[PMID]:25976635
[Au] Autor:Bellaw JL; Nielsen MK
[Ad] Endereço:M. H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA. Electronic address: jbe244@uky.edu.
[Ti] Título:Evaluation of Baermann apparatus sedimentation time on recovery of Strongylus vulgaris and S. edentatus third stage larvae from equine coprocultures.
[So] Source:Vet Parasitol;211(1-2):99-101, 2015 Jun 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Traditional methods of diagnosing equine Strongylinae infections require culturing feces, sedimenting the culture media in Baermann apparatuses, collecting the sediment, and morphologically identifying recovered third stage larvae. However, this method is plagued by low negative predictive values. This study evaluated sedimentation time within the Baermann apparatus by comparing larval recovery from the traditionally collected sediment, "sediment 1", and from the usually discarded remaining fluid contents, "sediment 2", of the Baermann apparatus after 12, 24, and 48 h. A grand total of 147,482 larvae were recovered and examined. Sedimentation time did not significantly influence total larval recovery. At all three durations, significantly more Cyathostominae and Strongylus vulgaris larvae were covered from sediment 1 than from sediment 2. However, less than 60% of all recovered Strongylus edentatus were recovered from sediment 1. As 95% of S. vulgaris larvae were always recovered from sediment 1, the need for collection and examination of the remaining fluid contents of the Baermann apparatus is obviated when performing coprocultures for diagnosis of S. vulgaris infections, and sedimentation for 12h is adequate. Approximately 70% of Cyathostominae were recovered in sediment 1 at all durations, suggesting that 12h of sedimentation is adequate, although there is a need for future research to evaluate the risk of selection bias at differing sedimentation times among individual cyathostomin species. In contrast to S. vulgaris, collecting and examining the entire contents of the Baermann apparatus may be necessary when an increased diagnostic sensitivity and negative predictive value is desired in diagnosing S. edentatus infections as only 38-61% of larvae were recovered from sediment 1 portion of the Baermann apparatus. This information will allow researchers and practitioners to make more informed decisions in choosing appropriate larval recovery techniques, balancing recovery, time, and effort.
[Mh] Termos MeSH primário: Infecções Equinas por Strongyloidea/parasitologia
Strongyloidea/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Meios de Cultura
Cavalos
Larva
Strongylus/isolamento & purificação
Fatores de Tempo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170413
[Lr] Data última revisão:
170413
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150516
[St] Status:MEDLINE


  10 / 167 MEDLINE  
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[PMID]:25358238
[Au] Autor:Nielsen MK; Vidyashankar AN; Bellaw J; Gravatte HS; Cao X; Rubinson EF; Reinemeyer CR
[Ad] Endereço:M.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA, martin.nielsen@uky.edu.
[Ti] Título:Serum Strongylus vulgaris-specific antibody responses to anthelmintic treatment in naturally infected horses.
[So] Source:Parasitol Res;114(2):445-51, 2015 Feb.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Strongylus vulgaris is the most pathogenic helminth parasite of horses, causing verminous endarteritis with thromboembolism and infarction. A serum enzyme-linked immunosorbent assay (ELISA) has been validated for detection of antibodies to an antigen produced by migrating larvae of this parasite. The aim was to evaluate ELISA responses to anthelmintic treatment in cohorts of naturally infected horses. Fifteen healthy horses harboring patent S. vulgaris infections were turned out for communal grazing in May 2013 (day 0). On day 55, horses were ranked according to ELISA titers and randomly allocated to the following three groups: no treatment followed by placebo pellets daily; ivermectin on day 60 followed by placebo pellets daily; or ivermectin on day 60 followed by daily pyrantel tartrate. Fecal and serum samples were collected at ∼28-day intervals until study termination on day 231. Increased ELISA values were observed for the first 53 days following ivermectin treatment. Titers were significantly reduced 80 days after ivermectin treatment. Horses receiving daily pyrantel tartrate maintained lower ELISA values from 137 days post ivermectin treatment until trial termination. These results illustrate that a positive ELISA result is indicative of either current or prior exposure to larval S. vulgaris infection within the previous 5 months.
[Mh] Termos MeSH primário: Anti-Helmínticos/uso terapêutico
Anticorpos Anti-Helmínticos/sangue
Doenças dos Cavalos/tratamento farmacológico
Ivermectina/uso terapêutico
Infecções por Strongylida/veterinária
Strongylus/imunologia
[Mh] Termos MeSH secundário: Animais
Estudos de Coortes
Ensaio de Imunoadsorção Enzimática/veterinária
Fezes/parasitologia
Doenças dos Cavalos/imunologia
Doenças dos Cavalos/parasitologia
Cavalos
Larva
Contagem de Ovos de Parasitas/veterinária
Distribuição Aleatória
Infecções por Strongylida/tratamento farmacológico
Infecções por Strongylida/imunologia
Infecções por Strongylida/parasitologia
Strongylus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Antibodies, Helminth); 70288-86-7 (Ivermectin)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141101
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-014-4201-5



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