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Pesquisa : B01.050.500.500.294.400.984 [Categoria DeCS]
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  1 / 207 MEDLINE  
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[PMID]:28315996
[Au] Autor:Cevallos JA; Okubo RP; Perlman SJ; Hallem EA
[Ad] Endereço:Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, CA, 90095, USA.
[Ti] Título:Olfactory Preferences of the Parasitic Nematode Howardula aoronymphium and its Insect Host Drosophila falleni.
[So] Source:J Chem Ecol;43(4):362-373, 2017 Apr.
[Is] ISSN:1573-1561
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many parasitic nematodes have an environmental infective stage that searches for hosts. Olfaction plays an important role in this process, with nematodes navigating their environment using host-emitted and environmental olfactory cues. The interactions between parasitic nematodes and their hosts are also influenced by the olfactory behaviors of the host, since host olfactory preferences drive behaviors that may facilitate or impede parasitic infection. However, how olfaction shapes parasite-host interactions is poorly understood. Here we investigated this question using the insect-parasitic nematode Howardula aoronymphium and its host, the mushroom fly Drosophila falleni. We found that both H. aoronymphium and D. falleni are attracted to mushroom odor and a subset of mushroom-derived odorants, but they have divergent olfactory preferences that are tuned to different mushroom odorants despite their shared mushroom environment. H. aoronymphium and D. falleni respond more narrowly to odorants than Caenorhabditis elegans and Drosophila melanogaster, consistent with their more specialized niches. Infection of D. falleni with H. aoronymphium alters its olfactory preferences, rendering it more narrowly tuned to mushroom odor. Our results establish H. aoronymphium-D. falleni as a model system for studying olfaction in the context of parasite-host interactions.
[Mh] Termos MeSH primário: Drosophila/fisiologia
Drosophila/parasitologia
Interações Hospedeiro-Parasita
Odorantes/análise
Olfato/fisiologia
Tylenchida/fisiologia
[Mh] Termos MeSH secundário: Animais
Dióxido de Carbono/química
Quimiotaxia
Meio Ambiente
Larva/parasitologia
Larva/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170320
[St] Status:MEDLINE
[do] DOI:10.1007/s10886-017-0834-z


  2 / 207 MEDLINE  
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[PMID]:28257464
[Au] Autor:Janssen T; Karssen G; Topalovic O; Coyne D; Bert W
[Ad] Endereço:Nematology Research Unit, Department of Biology, Ghent University, K.L. Ledeganckstraat, Ghent, Belgium.
[Ti] Título:Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis.
[So] Source:PLoS One;12(3):e0172190, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During sampling of several Coffea arabica plantations in Tanzania severe root galling, caused by a root-knot nematode was observed. From pure cultures, morphology and morphometrics of juveniles and females matched perfectly with Meloidogyne africana, whereas morphology of the males matched identically with those of Meloidogyne decalineata. Based on their Cox1 sequence, however, the recovered juveniles, females and males were confirmed to belong to the same species, creating a taxonomic conundrum. Adding further to this puzzle, re-examination of M. oteifae type material showed insufficient morphological evidence to maintain its status as a separate species. Consequently, M. decalineata and M. oteifae are synonymized with M. africana, which is herewith redescribed based on results of light and scanning electron microscopy, ribosomal and mitochondrial DNA sequences, isozyme electrophoresis, along with bionomic and cytogenetic features. Multi-gene phylogenetic analysis placed M. africana outside of the three major clades, together with M. coffeicola, M. ichinohei and M. camelliae. This phylogenetic position was confirmed by several morphological features, including cellular structure of the spermatheca, egg mass position, perineal pattern and head shape. Moreover, M. africana was found to be a polyphagous species, demonstrating that "early-branching" Meloidogyne spp. are not as oligophagous as had previously been assumed. Cytogenetic information indicates M. africana (2n = 21) and M. ardenensis (2n = 51-54) to be a triploid mitotic parthenogenetic species, revealing at least four independent origins of mitotic parthenogenesis within the genus Meloidogyne. Furthermore, M. mali (n = 12) was found to reproduce by amphimixis, indicating that amphimictic species with a limited number of chromosomes are widespread in the genus, potentially reflecting the ancestral state of the genus. The wide variation in chromosome numbers and associated changes in reproduction modes indicate that cytogenetic evolution played a crucial role in the speciation of root-knot nematodes and plant-parasitic nematodes in general.
[Mh] Termos MeSH primário: Coffea/parasitologia
Ciclo-Oxigenase 1/genética
Evolução Molecular
Filogenia
Tylenchida/genética
[Mh] Termos MeSH secundário: Animais
Classificação
DNA Mitocondrial/genética
Variação Genética
Raízes de Plantas/parasitologia
Especificidade da Espécie
Tanzânia
Tylenchida/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); EC 1.14.99.1 (Cyclooxygenase 1)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172190


  3 / 207 MEDLINE  
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[PMID]:28043333
[Au] Autor:Guo Q; Du G; Qi H; Zhang Y; Yue T; Wang J; Li R
[Ad] Endereço:College of Life Sciences, Qingdao University, Qingdao 266071, PR China. Electronic address: gqunqun@163.com.
[Ti] Título:A nematicidal tannin from Punica granatum L. rind and its physiological effect on pine wood nematode (Bursaphelenchus xylophilus).
[So] Source:Pestic Biochem Physiol;135:64-68, 2017 Jan.
[Is] ISSN:1095-9939
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ethanol extract of Punica granatum L. rind was tested to show significant nematicidal activity against pine wood nematode. Three nematicidal compounds were obtained from the ethanol extract by bioassay-guided fractionation and identified as punicalagin 1, punicalin 2, and corilagin 3 by mass and nuclear magnetic resonance spectral data analysis. Punicalagin 1 was most active against PWN among the purified compounds with the LC value of 307.08µM in 72h. According to the enzyme assays in vitro, punicalagin 1 could inhibit the activity of acetylcholinesterase, amylase and cellulase from PWN with IC value of 0.60mM, 0.96mM and 1.24mM, respectively. The morphological structures of PWNs treated by punicalagin 1 were greatly changed. These physiological effects of punicalagin 1 on PWN may helpful to elucidate its nematicidal mechanism.
[Mh] Termos MeSH primário: Antinematódeos/toxicidade
Taninos Hidrolisáveis/toxicidade
Extratos Vegetais/toxicidade
Punicaceae
Tylenchida/efeitos dos fármacos
[Mh] Termos MeSH secundário: Acetilcolinesterase/metabolismo
Amilases/antagonistas & inibidores
Animais
Antinematódeos/química
Celulase/antagonistas & inibidores
Inibidores da Colinesterase/química
Inibidores da Colinesterase/toxicidade
Glucosídeos/análise
Glucosídeos/toxicidade
Taninos Hidrolisáveis/análise
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Extratos Vegetais/química
Tylenchida/enzimologia
Tylenchida/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antinematodal Agents); 0 (Cholinesterase Inhibitors); 0 (Glucosides); 0 (Hydrolyzable Tannins); 0 (Plant Extracts); 62LOS9TW6D (corilagin); 65995-63-3 (punicalagin); 65995-64-4 (punicalin); EC 3.1.1.7 (Acetylcholinesterase); EC 3.2.1.- (Amylases); EC 3.2.1.4 (Cellulase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE


  4 / 207 MEDLINE  
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[PMID]:27785885
[Au] Autor:Proença DN; Grass G; Morais PV
[Ad] Endereço:CEMUC, University of Coimbra, Coimbra, Portugal.
[Ti] Título:Understanding pine wilt disease: roles of the pine endophytic bacteria and of the bacteria carried by the disease-causing pinewood nematode.
[So] Source:Microbiologyopen;6(2), 2017 Apr.
[Is] ISSN:2045-8827
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pine wilt disease (PWD) is one of the most destructive diseases in trees of the genus Pinus and is responsible for environmental and economic losses around the world. The only known causal agent of the disease is the pinewood nematode (PWN) Bursaphelenchus xylophilus. Despite that, bacteria belonging to several different genera have been found associated with PWN and their roles in the development of PWD have been suggested. Molecular methodologies and the new era of genomics have revealed different perspectives to the problem, recognizing the manifold interactions between different organisms involved in the disease. Here, we reviewed the possible roles of nematode-carried bacteria in PWD, what could be the definition of this group of microorganisms and questioned their origin as possible endophytes, discussing their relation within the endophytic community of pine trees. The diversity of the nematode-carried bacteria and the diversity of pine tree endophytes, reported until now, is revised in detail in this review. What could signify a synergetic effect with PWN harming the plant, or what could equip bacteria with functions to control the presence of nematodes inside the tree, is outlined as two possible roles of the microbial community in the etiology of this disease. An emphasis is put on the potential revealed by the genomic data of isolated organisms in their potential activities as effective tools in PWD management.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Endófitos/isolamento & purificação
Pinus/microbiologia
Pinus/parasitologia
Doenças das Plantas/microbiologia
Doenças das Plantas/parasitologia
Tylenchida/microbiologia
[Mh] Termos MeSH secundário: Animais
Antiprotozoários/metabolismo
Bactérias/genética
Proteínas de Bactérias/metabolismo
Endófitos/genética
Insetos Vetores/parasitologia
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Bacterial Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161028
[St] Status:MEDLINE
[do] DOI:10.1002/mbo3.415


  5 / 207 MEDLINE  
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[PMID]:27414986
[Au] Autor:Cheng L; Xu S; Xu C; Lu H; Zhang Z; Zhang D; Mu W; Liu F
[Ad] Endereço:Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, China.
[Ti] Título:Effects of trans-2-hexenal on reproduction, growth and behaviour and efficacy against the pinewood nematode, Bursaphelenchus xylophilus.
[So] Source:Pest Manag Sci;73(5):888-895, 2017 May.
[Is] ISSN:1526-4998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bursaphelenchus xylophilus is a serious quarantined pest that causes severe damage and major economic losses to pine forests. Because of the adverse effects of some traditional nematicides on humans and the environment, the search for new plant toxicants against these nematodes has intensified. Nematicidal activity of trans-2-hexenal, which is a six-carbon aldehyde present in many plants, was tested against the nematode. RESULTS: trans-2-Hexenal showed significant efficacy against B. xylophilus in a dose range of 349.5-699 g m by fumigation of pinewood logs. Additionally, it had significant nematicidal activity against different life stages of B. xylophilus in an in vitro test, with second-stage larvae (L2s) being the most sensitive, with an LC value of 9.87 µg mL at 48 h. Egg hatch was also significantly inhibited. Further studies revealed that trans-2-hexenal inhibited the reproductive activity of B. xylophilus, with negative effects on reproduction rate and egg numbers. Moreover, trans-2-hexenal reduced the body length of B. xylophilus. Respiratory rate and thrashing behaviour of B. xylophilus also decreased following treatment with this compound. CONCLUSION: trans-2-Hexenal had significant nematicidal activity against B. xylophilus, providing a basis for elucidation of the mode of action of trans-2-hexenal against plant-parasitic nematodes in future studies. © 2016 Society of Chemical Industry.
[Mh] Termos MeSH primário: Aldeídos/farmacologia
Comportamento Animal/efeitos dos fármacos
Tylenchida/efeitos dos fármacos
Tylenchida/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Fumigação
Locomoção/efeitos dos fármacos
Masculino
Oviposição/efeitos dos fármacos
Pinus/parasitologia
Reprodução/efeitos dos fármacos
Respiração/efeitos dos fármacos
Tylenchida/crescimento & desenvolvimento
Tylenchida/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aldehydes); 505-57-7 (2-hexenal)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160715
[St] Status:MEDLINE
[do] DOI:10.1002/ps.4360


  6 / 207 MEDLINE  
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[PMID]:28060317
[Au] Autor:Zhu N; Bai L; Schütz S; Liu B; Liu Z; Zhang X; Yu H; Hu J
[Ad] Endereço:Department of Forest Protection, Zhejiang Agricultural & Forestry University.
[Ti] Título:Observation and Quantification of Mating Behavior in the Pinewood Nematode, Bursaphelenchus xylophilus.
[So] Source:J Vis Exp;(118), 2016 Dec 25.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A method for observing and quantifying the mating behavior of the pinewood nematode, Bursaphelenchus xylophilus, was established under a stereomicroscope. To improve the mating efficiency of B. Xylophilus and to increase the chances of mating observation, virgin adults were cultured and used for the investigation. Eggs were obtained by keeping the nematodes in water and allowing the females to lay eggs for 10 min. The second-stage juveniles (J2) were synchronized by incubating the eggs for 24 h at 25 °C in the dark, and the early J4 were obtained by culturing the J2 with grey mold, Botrytis cinerea, for another 52 h. At this time point, most J4 nematodes could be clearly distinguished as being male or female using their genital morphology. The male and female J4 were collected and cultured separately in two different Petri dishes for 24 h to get virgin adult nematodes. A virgin male and a virgin female were paired in a drop of water in the well of a concave slide. The mating behavior was filmed with a video recorder under a stereomicroscope. The whole period of the mating process was 82.8 ±3.91 min (mean ±SE) and could be divided into 4 different phases: searching, contacting, copulating, and lingering. The mean minutes of duration were 21.8 ± 2.0, 28.0 ± 1.9, 25.8 ± 0.7 and 7.2 ± 0.5, respectively. Eleven sub-behaviors were described: cruising, approaching, encountering, touching, hooping, locating, attaching, ejaculating, separating, quiescence, and roaming. Interestingly, obvious intra-sexual competition was observed when one female was grouped with 3 males or one male with 3 females. This protocol is useful and valuable, not only in investigating the mating behavior of B. xylophilus, but also in acting as a reference for ethological studies of other nematodes.
[Mh] Termos MeSH primário: Microscopia
Comportamento Sexual Animal
Tylenchida/fisiologia
Gravação em Vídeo
[Mh] Termos MeSH secundário: Animais
Feminino
Fungos
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.3791/54842


  7 / 207 MEDLINE  
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[PMID]:28028099
[Au] Autor:Hartshorn JA; Chase KD; Galligan LD; Riggins JJ; Stephen FM
[Ad] Endereço:Division of Forestry, Minnesota Department of Natural Resources, 1201 E Hwy 2, Grand Rapids, MN 55744 (jessica.hartshorn@state.mn.us) jessica.hartshorn@state.mn.us.
[Ti] Título:Interactions Among Latitude, Nematode Parasitization, and Female Sirex nigricornis (Hymenoptera: Siricidae) Fitness.
[So] Source:Environ Entomol;45(6):1515-1520, 2016 12.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sirex nigricornis F. (Hymenoptera: Siricidae) is an innocuous pine-inhabiting woodwasp native to eastern North America, utilizing dead or dying pine trees as hosts. Although S. nigricornis F. does not cause economic damage, a closely related species, Sirex noctilio, was discovered in New York in 2004 and has continually spread throughout the northeastern United States and southern Canada, threatening the multi-billion-dollar pine timber industry of the southeastern United States and raising interest about potential interactions with native woodwasps and associated mortality agents. A non-sterilizing strain of the biological control agent, Deladenus siricidicola Bedding (Tylenchida: Neotylenchidae), was introduced along with S. noctilio but is not inhibiting the spread or establishment of S. noctilio A North American congener, Deladenus proximus Bedding, has been recently isolated from S. noctilio and shows promise as a biological control agent. To better understand the potential of D. proximus as a control agent for S. noctilio, we measured and dissected nearly 1,200 S. nigricornis females from Arkansas and Mississippi and evaluated differences among collection location with regard to nematode virulence, woodwasp body size, and egg load. Body size and egg load were related to collection location, and nematode infestation resulted in significantly smaller females who produced significantly fewer eggs. Female woodwasps, especially those collected in Arkansas, were often fully sterilized by nematodes, and a higher percent sterilization was inversely related to body size and fewer eggs. We propose field studies to test the nematode's ability to sterilize S. noctilio in the northeastern United States.
[Mh] Termos MeSH primário: Distribuição Animal
Himenópteros/parasitologia
Tylenchida/fisiologia
[Mh] Termos MeSH secundário: Animais
Arkansas
Tamanho Corporal
Feminino
Aptidão Genética
Geografia
Himenópteros/genética
Reprodução
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvw109


  8 / 207 MEDLINE  
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[PMID]:28001236
[Au] Autor:Santos FL; Souza AM; Dantas-Torres F
[Ad] Endereço:Laboratório de Patologia e Biointervenção, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil.
[Ti] Título:Meloidogyne eggs in human stool in Northeastern Brazil.
[So] Source:Rev Soc Bras Med Trop;49(6):802, 2016 Nov-Dec.
[Is] ISSN:1678-9849
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Mh] Termos MeSH primário: Fezes/parasitologia
Óvulo/ultraestrutura
Tylenchida
[Mh] Termos MeSH secundário: Animais
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170405
[Lr] Data última revisão:
170405
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE


  9 / 207 MEDLINE  
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[PMID]:27768814
[Au] Autor:Nascimento FX; Espada M; Barbosa P; Rossi MJ; Vicente CS; Mota M
[Ad] Endereço:Nemalab/ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Departamento de Biologia, Universidade de Évora, Núcleo da Mitra, Ap. 94, Évora, 7002-554, Portugal.
[Ti] Título:Non-specific transient mutualism between the plant parasitic nematode, Bursaphelenchus xylophilus, and the opportunistic bacterium Serratia quinivorans BXF1, a plant-growth promoting pine endophyte with antagonistic effects.
[So] Source:Environ Microbiol;18(12):5265-5276, 2016 Dec.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study is to understand the biological role of Serratia quinivorans BXF1, a bacterium commonly found associated with Bursaphelenchus xylophilus, the plant parasitic nematode responsible for pine wilt disease. Therefore, we studied strain BXF1 effect in pine wilt disease. We found that strain BXF1 promoted in vitro nematode reproduction. Moreover, the presence of bacteria led to the absence of nematode chitinase gene (Bxcht-1) expression, suggesting an effect for bacterial chitinase in nematode reproduction. Nevertheless, strain BXF1 was unable to colonize the nematode interior, bind to its cuticle with high affinity or protect the nematode from xenobiotic stress. Interestingly, strain BXF1 was able to promote tomato and pine plant-growth, as well as to colonize its interior, thus, acting like a plant-growth promoting endophyte. Consequently, strain BXF1 failed to induce wilting symptoms when inoculated in pine shoot artificial incisions. This bacterium also presented strong antagonistic activities against fungi and bacteria isolated from Pinus pinaster. Our results suggest that B. xylophilus does not possess a strict symbiotic community capable of inducing pine wilt disease symptoms as previously hypothesized. We show that bacteria like BXF1, which possess plant-growth promoting and antagonistic effects, may be opportunistically associated with B. xylophilus, possibly acquired from the bacterial endophytic community of the host pine.
[Mh] Termos MeSH primário: Endófitos/fisiologia
Pinus/microbiologia
Pinus/parasitologia
Doenças das Plantas/parasitologia
Serratia/fisiologia
Tylenchida/fisiologia
[Mh] Termos MeSH secundário: Animais
Endófitos/genética
Endófitos/isolamento & purificação
Pinus/crescimento & desenvolvimento
Serratia/genética
Serratia/isolamento & purificação
Simbiose
Tylenchida/genética
Tylenchida/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13568


  10 / 207 MEDLINE  
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[PMID]:27706584
[Au] Autor:Moraes Filho RM; Martins LS
[Ad] Endereço:Departamento de Agronomia da Universidade Federal Rural de Pernambuco, Recife, PE, Brasil romulommfilho@yahoo.com.br.
[Ti] Título:In silico comparative analysis of tylenchid nematode pectate lyases.
[So] Source:Genet Mol Res;15(3), 2016 Aug 19.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Phytoparasitic nematodes can infect a wide range of crop plants, and cause billions of dollars of agricultural losses each year. These parasites represent the largest source of biotic stress experienced by plants. The order Tylenchida comprises the most important parasitic nematodes, particularly the root-knot and cyst nematodes. These parasitic organisms obtain nutrients to support their development through complex interactions with their hosts. Plant-parasitic nematodes secrete a mixture of cell-wall degrading enzymes to facilitate migration through the plant root. Enzymes are secreted that degrade the principal cell-wall components, cellulose, hemi-cellulose, or pectin. Pectate lyases are important parasitism factors in plant-parasitic nematodes. These enzymes degrade polygalacturonic acid, which is a fundamental constituent of pectin of host cell walls. Thus, pectate lyases permit the penetration and colonization of plant host cells by parasites. Here, we analyzed 22 pectate lyase protein sequences from tylenchid nematode species. Our results revealed great variation in the isoelectric points of pectate lyases, and groups of acidic and alkaline proteins that may have distinct enzymatic activities were identified. Phylogenetic analysis also revealed the presence of two main groups of pectate lyases with distinct chemical properties. Seven conserved motifs were identified, but only five were present in all sequences. Results of the molecular docking analysis revealed differences in the predicted interaction sites in the pectate lyases from the two groups. These results may provide a theoretical basis for future studies of host plant resistance to nematode infection.
[Mh] Termos MeSH primário: Proteínas de Helminto/química
Polissacarídeo-Liase/química
Tylenchida/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sítios de Ligação
Sequência Conservada
Produtos Agrícolas/parasitologia
Resistência à Doença
Simulação de Acoplamento Molecular
Filogenia
Doenças das Plantas/parasitologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Helminth Proteins); EC 4.2.2.- (Polysaccharide-Lyases); EC 4.2.2.2 (pectate lyase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161006
[St] Status:MEDLINE
[do] DOI:10.4238/gmr.15038402



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