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  1 / 193 MEDLINE  
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[PMID]:28686662
[Au] Autor:Ng-Nguyen D; Stevenson MA; Dorny P; Gabriël S; Vo TV; Nguyen VT; Phan TV; Hii SF; Traub RJ
[Ad] Endereço:Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Victoria, Australia.
[Ti] Título:Comparison of a new multiplex real-time PCR with the Kato Katz thick smear and copro-antigen ELISA for the detection and differentiation of Taenia spp. in human stools.
[So] Source:PLoS Negl Trop Dis;11(7):e0005743, 2017 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Taenia solium, the cause of neurocysticercosis (NCC), has significant socioeconomic impacts on communities in developing countries. This disease, along with taeniasis is estimated to infect 2.5 to 5 million people globally. Control of T. solium NCC necessitates accurate diagnosis and treatment of T. solium taeniasis carriers. In areas where all three species of Taenia tapeworms (T. solium, Taenia saginata and Taenia asiatica) occur sympatrically, conventional microscope- and copro-antigen based diagnostic methods are unable to distinguish between these three Taenia species. Molecular diagnostic tools have been developed to overcome this limitation; however, conventional PCR-based techniques remain unsuitable for large-scale deployment in community-based surveys. Moreover, a real-time PCR (qPCR) for the discrimination of all three species of Taenia in human stool does not exist. This study describes the development and validation of a new triplex Taq-Man probe-based qPCR for the detection and discrimination of all three Taenia human tapeworms in human stools collected from communities in the Central Highlands of Vietnam. The diagnostic characteristics of the test are compared with conventional Kato Katz (KK) thick smear and copro-antigen ELISA (cAgELISA) method utilizing fecal samples from a community based cross-sectional study. Using this new multiplex real-time PCR we provide an estimate of the true prevalence of taeniasis in the source population for the community based cross-sectional study. METHODOLOGY/PRINCIPAL FINDINGS: Primers and TaqMan probes for the specific amplification of T. solium, T. saginata and T. asiatica were designed and successfully optimized to target the internal transcribed spacer I (ITS-1) gene of T. solium and the cytochrome oxidase subunit I (COX-1) gene of T. saginata and T. asiatica. The newly designed triplex qPCR (T3qPCR) was compared to KK and cAgELISA for the detection of Taenia eggs in stool samples collected from 342 individuals in Dak Lak province, Central Highlands of Vietnam. The overall apparent prevalence of taeniasis in Dak Lak province was 6.72% (95% confidence interval (CI) [3.94-9.50]) in which T. solium accounted for 1.17% (95% CI [0.37-3.17]), according to the T3qPCR. There was sympatric presence of T. solium, T. saginata and T. asiatica. The T3qPCR proved superior to KK and cAgELISA for the detection and differentiation of Taenia species in human feces. Diagnostic sensitivities of 0.94 (95% credible interval (CrI) [0.88-0.98]), 0.82 (95% CrI [0.58-0.95]) and 0.52 (95% CrI [0.07-0.94]), and diagnostic specificities of 0.98 (95% CrI [0.94-1.00]), 0.91 (95% CrI [0.85-0.96]) and 0.99 (95% CrI [0.96-1.00]) were estimated for the diagnosis of taeniasis for the T3qPCR, cAgELISA and KK thick smear in this study, respectively. CONCLUSIONS: T3qPCR is not only superior to the KK thick smear and cAgELISA in terms of diagnostic sensitivity and specificity, but it also has the advantage of discriminating between species of Taenia eggs in stools. Application of this newly developed T3qPCR has identified the existence of all three human Taenia tapeworms in Dak Lak province and proves for the first time, the existence of T. asiatica in the Central Highlands and the south of Vietnam.
[Mh] Termos MeSH primário: Fezes/parasitologia
Reação em Cadeia da Polimerase Multiplex/métodos
Neurocisticercose/diagnóstico
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Animais
Estudos Transversais
Primers do DNA
Ensaio de Imunoadsorção Enzimática/métodos
Seres Humanos
Neurocisticercose/parasitologia
Sensibilidade e Especificidade
Taenia saginata/isolamento & purificação
Taenia solium/isolamento & purificação
Vietnã
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005743


  2 / 193 MEDLINE  
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[PMID]:28284223
[Au] Autor:Sanpool O; Rodpai R; Intapan PM; Sadaow L; Thanchomnang T; Laymanivong S; Maleewong W; Yamasaki H
[Ad] Endereço:Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
[Ti] Título:Genetic diversity of Taenia saginata (Cestoda: Cyclophyllidea) from Lao People's Democratic Republic and northeastern Thailand based on mitochondrial DNA.
[So] Source:Parasit Vectors;10(1):141, 2017 Mar 11.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Taenia saginata is a tapeworm found in cattle worldwide. Analysis of genetic diversity in different geographical populations of T. saginata not only helps to understand the origin, transmission and spread of this organism, but also to evaluate the selection pressures acting on T. saginata and how it is responding to them. However, there are few reports of the genetic variability of T. saginata populations in different regions of the world, including Lao PDR and Thailand. We report the genetic diversity of T. saginata populations in Lao PDR and northeastern Thailand together with sequences of T. saginata from other countries deposited in GenBank. RESULTS: Mitochondrial cox1 sequence analysis revealed that 15 and 8 haplotypes were identified in 30 and 21 T. saginata isolates from Lao PDR and northeastern Thailand, respectively. Fifty-three haplotypes were identified from 98 sequences. Phylogenetic tree and haplotype network analyses revealed that global isolates of T. saginata were genetically divided into five groups (A, B, C1, C2 and D). Taenia saginata isolates from Lao PDR and northeastern Thailand belonged to either Group A or B. Taenia saginata from western Thailand clustered in groups C1, C2 and D, and populations from the northeast and western Thailand were found to be genetically distinct. Taenia saginata isolates in Lao PDR and Thailand were also found to be genetically diverse but the degree of genetic differentiation was low. CONCLUSIONS: Taenia saginata populations from Lao PDR and northeastern Thailand are genetically distinct from the population in western Thailand and it is proposed that T. saginata has been dispersed by different transmission routes in Southeast Asia.
[Mh] Termos MeSH primário: Doenças dos Bovinos/parasitologia
DNA de Helmintos/genética
DNA Mitocondrial/genética
Variação Genética
Taenia saginata/genética
Teníase/parasitologia
Teníase/veterinária
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/epidemiologia
Seres Humanos
Laos
Filogenia
Filogeografia
Taenia saginata/classificação
Taenia saginata/isolamento & purificação
Teníase/epidemiologia
Tailândia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth); 0 (DNA, Mitochondrial)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2079-7


  3 / 193 MEDLINE  
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[PMID]:27748211
[Au] Autor:Nunes DS; Gonzaga HT; Ribeiro VS; Cunha-Júnior JP; Costa-Cruz JM
[Ad] Endereço:Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia,Av. Pará 1720,38400-902, Uberlândia, MG,Brazil.
[Ti] Título:Usefulness of gel filtration fraction as potential biomarker for neurocysticercosis in serum: towards a new diagnostic tool.
[So] Source:Parasitology;144(4):426-435, 2017 Apr.
[Is] ISSN:1469-8161
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:There is an increasing interest in improving neurocysticercosis (NCC) diagnosis through the search of new and alternative antigenic sources, as those obtained from heterologous antigens. The aim of this study was to obtain potential biomarkers for NCC diagnosis after gel filtration chromatography [gel filtration fraction (GFF)] from the total saline extract (SE) from Taenia saginata metacestodes, followed by protein identification and application in immunodiagnostic. SE and GFF proteic profiles were characterized in gel electrophoresis, and diagnostic performance was verified by testing 160 serum samples through enzyme-linked immunosorbent assay and immunoblotting. Sensitivity (Se), specificity (Sp) and other diagnostic parameters were calculated. Polypeptides of interest in the diagnosis of human NCC present at GFF were analysed by mass spectrometry (MS) and B-cell epitopes were predicted. GFF had the best diagnostic parameters: Se 93·3%; Sp 93%; AUC 0·990; LR+ = 13·42 and LR- = 0·07, and proved to be useful reacting with serum samples in immunoblotting. Proteic profile ranged from 64 to 68 kDa and enolase and calcium binding protein calreticulin precursor were identified after MS. The enolase and calcium-binding protein calreticulin precursor showed 18 and 10 predicted B-cell epitopes, respectively. In conclusion we identified important markers in the GFF with high efficiency to diagnose NCC.
[Mh] Termos MeSH primário: Cromatografia em Gel/métodos
Proteínas de Helminto/metabolismo
Neurocisticercose/sangue
Neurocisticercose/diagnóstico
Taenia saginata/metabolismo
[Mh] Termos MeSH secundário: Animais
Biomarcadores/sangue
Fracionamento Químico
Ensaio de Imunoadsorção Enzimática
Epitopos de Linfócito B
Proteínas de Helminto/sangue
Proteínas de Helminto/genética
Seres Humanos
Programas de Rastreamento
Modelos Moleculares
Neurocisticercose/parasitologia
Conformação Proteica
Taenia saginata/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Epitopes, B-Lymphocyte); 0 (Helminth Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE
[do] DOI:10.1017/S0031182016001839


  4 / 193 MEDLINE  
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[PMID]:28292157
[Au] Autor:Najih M; Laraqui H; Njoumi N; Mouhafid F; Moujahid M; Ehirchiou A; Zentar A
[Ad] Endereço:Department of Surgery, Mohamed V Military Hospital, Mohamed V, Souissi University, Rabat, Morocco.
[Ti] Título:Taenia saginata: an unusual cause of post-appendectomy faecal fistula.
[So] Source:Pan Afr Med J;25:200, 2016.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:eng
[Ab] Resumo:Post-appendectomy faecal fistula is a rare surgical complication, associated with significant morbidity. Taenia saginata infestation is one of the most common cestode infestation in the gastrointestinal tract. It makes many complications as obstruction, perforation, anastomotic leakage or appendicular stump dehiscence. The objective of our study is to report a very rare case of post appendectomy faecal fistula caused by taenia saginata infestation and was successfully treated conservatively.
[Mh] Termos MeSH primário: Apendicectomia
Fístula Intestinal/parasitologia
Taenia saginata/isolamento & purificação
Teníase/diagnóstico
[Mh] Termos MeSH secundário: Animais
Feminino
Seres Humanos
Meia-Idade
Teníase/parasitologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.2016.25.200.11003


  5 / 193 MEDLINE  
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[PMID]:28091386
[Au] Autor:Mirzaei M; Nematolahi A; Ashrafihelan J; Rezaei H
[Ad] Endereço:Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran. dr_mirzaie_mo@yahoo.com.
[Ti] Título:Prevalence of Infection with the Larval Form of the Cestode Parasite Taenia saginata in Cattle in Northwest Iran and its Zoonotic Importance.
[So] Source:Turkiye Parazitol Derg;40(4):190-193, 2016 Dec.
[Is] ISSN:2146-3077
[Cp] País de publicação:Turkey
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Bovine cysticercosis is a cattle infection caused by a tapeworm, Taenia saginata. While the condition is relatively innocuous, the parasite infects the small intestine of humans in its mature stage and causes a few specific symptoms such as abdominal pain and nausea. METHODS: Between February 2013 and February 2014, a total of 640 cattle were randomly selected from all the cattle sent to the abattoir, and some internal organs and skeletal muscles of these cattle were inspected. RESULTS: Overall, 11 (1.71%) cattle were infected with the larval form of the cestode parasite T. saginata. In addition, the infection was more prevalent in cattle aged above 12 months than in those aged below 12 months [10 (2.06%) vs. 1 (0.64%)]. The prevalence of infection was significantly higher in female animals [8 (3.72%)] than in male animals [3 (0.70%)] (p<0.05). However, no significant difference was found between the rates in the 2 age groups or in different seasons. While the infections were detected in several visceral organs, no significant difference was found between their infection rates. CONCLUSION: The comparatively high prevalence of Cysticercus bovis infection in the cattle in Tabriz, Iran, may contribute to economic and health problems in the country's meat industry. On the other hand, the role of public health education in C. bovis infection control cannot be neglected.
[Mh] Termos MeSH primário: Criação de Animais Domésticos
Doenças dos Bovinos/epidemiologia
Cisticercose/veterinária
Taenia saginata/isolamento & purificação
Zoonoses
[Mh] Termos MeSH secundário: Matadouros
Animais
Bovinos
Doenças dos Bovinos/parasitologia
Cisticercose/epidemiologia
Feminino
Seres Humanos
Irã (Geográfico)/epidemiologia
Larva
Masculino
Carne/parasitologia
Prevalência
Zoonoses/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170117
[St] Status:MEDLINE
[do] DOI:10.5152/tpd.2016.4776


  6 / 193 MEDLINE  
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[PMID]:27931921
[Au] Autor:Marshall LR; Prakashbabu BC; Ferreira JP; Buzdugan SN; Stärk KDC; Guitian J
[Ad] Endereço:Veterinary Epidemiology, Economics and Public Health Group, Department of Production and Population Health, Royal Veterinary College, Hatfield AL9 7TA, United Kingdom.
[Ti] Título:Risk factors for Taenia saginata cysticercus infection in cattle in the United Kingdom: A farm-level case-control study and assessment of the role of movement history, age and sex.
[So] Source:Prev Vet Med;135:1-8, 2016 Dec 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bovine cysticercosis is caused by Taenia saginata cysticercus, the larval stage of the human tapeworm Taenia saginata. Recent European initiatives have highlighted the poor sensitivity of current surveillance for this parasite in cattle at slaughter; calling for more targeted, risk based and cost effective methods of T. saginata cysticercus detection. The aim of this study was to provide evidence that could inform such improved meat inspection activities in the United Kingdom (UK). The study included three components: (i) a farm-level case control study; (ii) the characterization of the network of movements of T. saginata cysticercus infected and non-infected animals, and an assessment of the strength of association between having passed through a farm that had previously originated an infected animal and the risk of infection; (iii) the assessment of the relationship between bovine age and gender and risk of infection. Abattoir records and cattle movement history data were used to identify farms of likely acquisition of infection (case farms) and a suitable control group. A questionnaire was used to gather farm-level characteristics and logistic regression was carried out to identify farm-level risk factors for the production of cattle found to be infected at slaughter. The case-control study provided evidence that farms situated close to a permanent potential source of human faecal contamination, and farms which used manure from animals other than cattle, were at higher risk of producing cattle later found to be infected with T. saginata cysticercus at slaughter. No other farm characteristics were identified as a risk factor for this. Analysis of the networks of animal movements showed that some individual farms played a key role as a source of T. saginata cysticercus infection; it was estimated that cattle with a history of being on a farm which previously appeared in the movement history of an infected animal were 4.27 times (P<0.001; 95% CI: 3.3-5.52) more likely to be diagnosed with T. saginata cysticercus infection at meat inspection. Male cattle aged 20 months or younger at the time of slaughter were found at lower risk of T. saginata cysticercus infection by comparison to other sex or age groups of cattle. These results, in combination with the consultation of experts and stakeholders, led to the conclusion that abattoir-based surveillance in low T. saginata cysticercus prevalence settings, such as Great Britain, could be made more targeted by stratifying cattle based on their individual movement history, sex and age characteristics.
[Mh] Termos MeSH primário: Doenças dos Bovinos/epidemiologia
Cisticercose/veterinária
Parasitologia de Alimentos
Carne/parasitologia
Taenia saginata/isolamento & purificação
[Mh] Termos MeSH secundário: Matadouros
Fatores Etários
Animais
Estudos de Casos e Controles
Bovinos
Doenças dos Bovinos/parasitologia
Cisticercose/epidemiologia
Cisticercose/parasitologia
Cysticercus/isolamento & purificação
Fazendas
Feminino
Inspeção de Alimentos/normas
Masculino
Prevalência
Fatores de Risco
Fatores Sexuais
Taenia saginata/crescimento & desenvolvimento
Transportes
Reino Unido/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE


  7 / 193 MEDLINE  
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[PMID]:27769720
[Au] Autor:Solano D; Navarro JC; León-Reyes A; Benítez-Ortiz W; Rodríguez-Hidalgo R
[Ad] Endereço:Centro Internacional de Zoonosis (CIZ), Universidad Central del Ecuador, Quito, Ecuador; Carrera Biotecnología, Universidad de las Fuerzas Armadas (ESPE), Quito, Ecuador.
[Ti] Título:Molecular analyses reveal two geographic and genetic lineages for tapeworms, Taenia solium and Taenia saginata, from Ecuador using mitochondrial DNA.
[So] Source:Exp Parasitol;171:49-56, 2016 Dec.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tapeworms Taenia solium and Taenia saginata are the causative agents of taeniasis/cysticercosis. These are diseases with high medical and veterinary importance due to their impact on public health and rural economy in tropical countries. The re-emergence of T. solium as a result of human migration, the economic burden affecting livestock industry, and the large variability of symptoms in several human cysticercosis, encourage studies on genetic diversity, and the identification of these parasites with molecular phylogenetic tools. Samples collected from the Ecuadorian provinces: Loja, Guayas, Manabí, Tungurahua (South), and Imbabura, Pichincha (North) from 2000 to 2012 were performed under Maximum Parsimony analyses and haplotype networks using partial sequences of mitochondrial DNA, cytochrome oxidase subunit I (COI) and NADH subunit I (NDI), from Genbank and own sequences of Taenia solium and Taenia saginata from Ecuador. Both species have shown reciprocal monophyly, which confirms its molecular taxonomic identity. The COI and NDI genes results suggest phylogenetic structure for both parasite species from south and north of Ecuador. In T. solium, both genes gene revealed greater geographic structure, whereas in T. saginata, the variability for both genes was low. In conclusion, COI haplotype networks of T. solium suggest two geographical events in the introduction of this species in Ecuador (African and Asian lineages) and occurring sympatric, probably through the most common routes of maritime trade between the XV-XIX centuries. Moreover, the evidence of two NDI geographical lineages in T. solium from the north (province of Imbabura) and the south (province of Loja) of Ecuador derivate from a common Indian ancestor open new approaches for studies on genetic populations and eco-epidemiology.
[Mh] Termos MeSH primário: DNA Mitocondrial
Variação Genética
Taenia saginata/genética
Taenia solium/genética
Teníase/parasitologia
[Mh] Termos MeSH secundário: Animais
Equador/epidemiologia
Complexo IV da Cadeia de Transporte de Elétrons/genética
Fluxo Gênico
Haplótipos
NADH Desidrogenase/genética
Filogenia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Taenia saginata/classificação
Taenia solium/classificação
Teníase/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); EC 1.6.99.3 (NADH Dehydrogenase); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170112
[Lr] Data última revisão:
170112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE


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[PMID]:27760308
[Au] Autor:Guimarães-Peixoto RPM; Pinto PSA; Santos MR; Polêto MD; Silva LF; Silva-Júnior A
[Ad] Endereço:Universidade Federal de Viçosa, Departamento de Veterinária, Laboratório de Inspeção de Produtos de Origem Animal, Campus Universitário, Viçosa, Minas Gerais, Brazil. Electronic address: rafaella.peixoto@ufv.br.
[Ti] Título:Evaluation of a synthetic peptide from the Taenia saginata 18kDa surface/secreted oncospheral adhesion protein for serological diagnosis of bovine cysticercosis.
[So] Source:Acta Trop;164:463-468, 2016 Dec.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bovine cysticercosis is a zoonotic infection widely spread throughout Brazil, creating a burden on hygiene maintenance and the economy. Diagnosis of cysticercosis usually relies on post mortem inspection of carcasses in slaughterhouses. This detection method provides only low sensitivity. Recent advancements have improved the performance of serologic tests, such as ELISA, providing greater sensitivity and specificity. The objective of the current study was to identify and evaluate a synthetic peptide derived from the Taenia saginata 18kDa oncospheric surface protein for the diagnosis of bovine cysticercosis in ELISA. Test performance of the identified peptide was compared to an ELISA based on a heterologous crude Taenia crassiceps antigen (Tcra), widely used for the sero-diagnosis of bovine cysticercosis. Based on the primary sequence of an in silico structural model of the 18kDa protein, an epitope region designated EP1 was selected (46-WDTKDMAGYGVKKIEV-61). The peptide derived from this region yielded 91.6% (CI=80-96%) sensitivity and 90% (CI=82-95%) specificity when used in an ELISA, whereas the crude antigen yielded 70% (CI=56-8%) sensitivity and 82% (CI=73-89%) specificity. Thus, we conclude that EP1 has higher diagnostic potential for detecting bovine cysticercosis than the crude antigen Tcra.
[Mh] Termos MeSH primário: Anticorpos Anti-Helmínticos/imunologia
Antígenos de Helmintos/imunologia
Doenças dos Bovinos/diagnóstico
Cisticercose/diagnóstico
Taenia saginata/imunologia
[Mh] Termos MeSH secundário: Animais
Brasil
Bovinos
Ensaio de Imunoadsorção Enzimática/métodos
Seres Humanos
Proteínas de Membrana
Sensibilidade e Especificidade
Testes Sorológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Membrane Proteins)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE


  9 / 193 MEDLINE  
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[PMID]:27650793
[Au] Autor:Canaval Zuleta HJ
[Ad] Endereço:Digestivo, Hospital Son Llatzer, España.
[Ti] Título:Author´s reply: About human taeniasis and Taenia saginata diagnosis by endoscopy.
[So] Source:Rev Esp Enferm Dig;108(10):684-685, 2016 Oct.
[Is] ISSN:1130-0108
[Cp] País de publicação:Spain
[La] Idioma:eng
[Ab] Resumo:Dear editor. We have published an article about the role of the endoscopy in the Taeniosis. I will try to clarify some doubts and misinformation about it, as well Galan-Puchades MT suggests.
[Mh] Termos MeSH primário: Taenia saginata
Teníase
[Mh] Termos MeSH secundário: Animais
Endoscopia
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160922
[St] Status:MEDLINE


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[PMID]:27523940
[Au] Autor:Jansen F; Dorny P; Berkvens D; Van Hul A; Van den Broeck N; Makay C; Praet N; Gabriël S
[Ad] Endereço:Department of Biomedical Sciences, Institute of Tropical Medicine, 155 Nationalestraat, B-2000 Antwerp, Belgium; Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, 133 Salisburylaan, B-9820 Merelbeke, Belgium. Electronic address: fjansen@itg.be.
[Ti] Título:Assessment of the repeatability and border-plate effects of the B158/B60 enzyme-linked-immunosorbent assay for the detection of circulating antigens (Ag-ELISA) of Taenia saginata.
[So] Source:Vet Parasitol;227:69-72, 2016 Aug 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The monoclonal antibody-based circulating antigen detecting ELISA (B158/B60 Ag-ELISA) has been used elaborately in several studies for the diagnosis of human, bovine and porcine cysticercosis. Interpretation of test results requires a good knowledge of the test characteristics, including the repeatability and the effect of the borders of the ELISA plates. Repeatability was tested for 4 antigen-negative and 5 antigen-positive reference bovine serum samples by calculating the Percentage Coefficient of Variation (%CV) within and between plates, within and between runs, overall, for two batches of monoclonal antibodies and by 2 laboratory technicians. All CV values obtained were below 20% (except one: 24.45%), which indicates a good repeatability and a negligible technician error. The value of 24.45% for indicating the variability between batches of monoclonal antibodies for one positive sample is still acceptable for repeatability measures. Border effects were determined by calculating the %CV values between the inner and outer wells of one plate for 2 positive serum samples. Variability is a little more present in the outer wells but this effect is very small and no significant border effect was found.
[Mh] Termos MeSH primário: Antígenos de Helmintos/sangue
Doenças dos Bovinos/parasitologia
Ensaio de Imunoadsorção Enzimática/veterinária
Taenia saginata/metabolismo
Teníase/veterinária
[Mh] Termos MeSH secundário: Animais
Antígenos de Helmintos/isolamento & purificação
Bovinos
Doenças dos Bovinos/sangue
Doenças dos Bovinos/diagnóstico
Ensaio de Imunoadsorção Enzimática/métodos
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Teníase/sangue
Teníase/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Helminth)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160816
[St] Status:MEDLINE



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