Base de dados : MEDLINE
Pesquisa : B01.050.500.500.736.715.408.380 [Categoria DeCS]
Referências encontradas : 751 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 76 ir para página                         

  1 / 751 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28973017
[Au] Autor:Khan MAH; Ullah R; Rehman A; Rehman L; P A AS; Abidi SMA
[Ad] Endereço:Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, India.
[Ti] Título:Immunolocalization and immunodetection of the excretory/secretory (ES) antigens of Fasciola gigantica.
[So] Source:PLoS One;12(10):e0185870, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The digenetic trematode Fasciola gigantica is a parasite of great agricultural and economic importance. Along with Fasciola hepatica, F. gigantica incurs huge economic losses to the agricultural sector. Because of unavailability of an effective and commercial vaccine, the earliest diagnosis of the disease is the only way to control the disease. The conventional coprological techniques are able to detect the disease only after the parasites get matured and starts releasing their eggs with the faeces of host, therefore prepatent infection remain undiagnosed. The alternative method is by serological tests that uses circulatory antigens. Despite high sensitivity, their reliability is quite low because of the common antigens shared between different helminth parasites. To overcome this, investigation was shifted to identify the copro-antigens which could be more sensitive and reliable. In the present study, we tried to identify some of the immunodominant proteins from the Excretory Secretory (ES) product of F. gigantica which can be further characterized and used for early detection of infection and also as drug and vaccine candidates. The ES products of F. gigantica were collected and used for raising the polyclonal antibody in rabbit. The polypeptide profile was generated as well as immunogenic polypeptides were identified. The Source of ES antigen was immunolocalized using confocal microscopy and dot blot assay was performed to diagnose field infection. The polypeptide profile of ES products revealed a total of 24 polypeptides out of which 12 immunogenic polypeptides were identified by western blotting. Confocal micrographs showed the immunolocalization of antigens in the intestinal caecae, vitalline glands, gonads as well as in the tegument of the worm. The dot blot assay confirmed the utility of ES products for the detection of field infection. Subsequently, cross reactivity was found negative with Gigantocotyle explanatum; an amphitome parasite of same habitat. However, the cross reactivity with other helminths needs to be worked out.
[Mh] Termos MeSH primário: Antígenos de Helmintos/imunologia
Fasciola hepatica/imunologia
Fasciola/imunologia
Fasciolíase/parasitologia
[Mh] Termos MeSH secundário: Animais
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Helminth)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185870


  2 / 751 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28915255
[Au] Autor:Calvani NED; Windsor PA; Bush RD; Slapeta J
[Ad] Endereço:Laboratory of Veterinary Parasitology, Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camperdown, New South Wales, Australia.
[Ti] Título:Scrambled eggs: A highly sensitive molecular diagnostic workflow for Fasciola species specific detection from faecal samples.
[So] Source:PLoS Negl Trop Dis;11(9):e0005931, 2017 Sep.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Fasciolosis, due to Fasciola hepatica and Fasciola gigantica, is a re-emerging zoonotic parasitic disease of worldwide importance. Human and animal infections are commonly diagnosed by the traditional sedimentation and faecal egg-counting technique. However, this technique is time-consuming and prone to sensitivity errors when a large number of samples must be processed or if the operator lacks sufficient experience. Additionally, diagnosis can only be made once the 12-week pre-patent period has passed. Recently, a commercially available coprological antigen ELISA has enabled detection of F. hepatica prior to the completion of the pre-patent period, providing earlier diagnosis and increased throughput, although species differentiation is not possible in areas of parasite sympatry. Real-time PCR offers the combined benefits of highly sensitive species differentiation for medium to large sample sizes. However, no molecular diagnostic workflow currently exists for the identification of Fasciola spp. in faecal samples. METHODOLOGY/PRINCIPAL FINDINGS: A new molecular diagnostic workflow for the highly-sensitive detection and quantification of Fasciola spp. in faecal samples was developed. The technique involves sedimenting and pelleting the samples prior to DNA isolation in order to concentrate the eggs, followed by disruption by bead-beating in a benchtop homogeniser to ensure access to DNA. Although both the new molecular workflow and the traditional sedimentation technique were sensitive and specific, the new molecular workflow enabled faster sample throughput in medium to large epidemiological studies, and provided the additional benefit of speciation. Further, good correlation (R2 = 0.74-0.76) was observed between the real-time PCR values and the faecal egg count (FEC) using the new molecular workflow for all herds and sampling periods. Finally, no effect of storage in 70% ethanol was detected on sedimentation and DNA isolation outcomes; enabling transport of samples from endemic to non-endemic countries without the requirement of a complete cold chain. The commercially-available ELISA displayed poorer sensitivity, even after adjustment of the positive threshold (65-88%), compared to the sensitivity (91-100%) of the new molecular diagnostic workflow. CONCLUSIONS/SIGNIFICANCE: Species-specific assays for sensitive detection of Fasciola spp. enable ante-mortem diagnosis in both human and animal settings. This includes Southeast Asia where there are potentially many undocumented human cases and where post-mortem examination of production animals can be difficult. The new molecular workflow provides a sensitive and quantitative diagnostic approach for the rapid testing of medium to large sample sizes, potentially superseding the traditional sedimentation and FEC technique and enabling surveillance programs in locations where animal and human health funding is limited.
[Mh] Termos MeSH primário: Doenças dos Bovinos/diagnóstico
Fasciola/isolamento & purificação
Fasciolíase/diagnóstico
Fezes/parasitologia
Técnicas de Diagnóstico Molecular
Reação em Cadeia da Polimerase em Tempo Real
Doenças dos Ovinos/diagnóstico
[Mh] Termos MeSH secundário: Animais
Antígenos de Helmintos/genética
Antígenos de Helmintos/isolamento & purificação
Bovinos
Doenças dos Bovinos/parasitologia
Ensaio de Imunoadsorção Enzimática
Fasciola/genética
Fasciola hepatica/isolamento & purificação
Fasciolíase/parasitologia
Seres Humanos
Contagem de Ovos de Parasitas
Sensibilidade e Especificidade
Ovinos
Doenças dos Ovinos/parasitologia
Fluxo de Trabalho
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Helminth)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005931


  3 / 751 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28215861
[Au] Autor:Dermauw V; Meas S; Chea B; Onkelinx T; Sorn S; Holl D; Charlier J; Vercruysse J; Dorny P
[Ad] Endereço:Department of Biomedical Sciences, Institute of Tropical Medicine, Nationalestraat 155, B-2000, Antwerpen, Belgium. Electronic address: vdermauw@itg.be.
[Ti] Título:Effects of anthelmintic treatment and feed supplementation on parasite infections and morbidity parameters in Cambodian cattle.
[So] Source:Vet Parasitol;235:113-122, 2017 Feb 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Helminth infections are the cause of morbidity in Cambodian cattle but other factors such as nutritional deficiencies and concurrent diseases may enhance the effects of parasites. The present study aimed to investigate the impact of anthelmintic treatment, feed supplementation, or both on gastrointestinal strongyle (GIS) and trematode infections as well as on morbidity parameters in Cambodian village cattle. At the beginning of the dry season, cattle populations in six villages were randomly assigned to a group: (A) receiving anthelmintic treatment (ivermectin+clorsulon) at week 0; (P) feed pellet supplementation during week 0-13 or both (AP). On five visits (week 0-29), faecal and blood samples were obtained for parasitological examination and haematocrit determination, respectively. Body condition (BCS), hind quarter fouling (HQFS), diarrhoea (DS), and conjunctiva colour (FAMACHA ) were scored and heart girth circumference was determined. To investigate the impact of treatment over time (week 0-29), a mixed model was used with treatment, time, and their interaction as fixed effects, and animal and village as random factors. At baseline, the proportion of GIS positive animals was high (67.9%), whereas trematode infections were low (Paramphistomum: 8.8%; Fasciola: 2.6%). Very thin to emaciated cattle (BCS 1-2) were more prevalent (11.4%) and FAMACHA scores of ≤3 or below (65.8%) less prevalent than in an earlier study in the region. A Time ⨯ Treatment interaction was present for faecal egg counts (FEC) of GIS, GIS prevalence (both p<0.0001), PCV (p=0.0034), DS (p=0.0086) and HQFS (p=0.0241). For GIS FEC, treatment groups differed at a specific time point, with levels of treatment group P being higher than in A at week 6 (p=0.0054). For Paramphistomum prevalence as well as FAMACHA scoring, heart girth and BCS, the interaction between treatment and time was not significant, yet, time in itself had a significant impact on all (p<0.0001). The beneficial effects of protein supplementation were unclear from the current study.
[Mh] Termos MeSH primário: Anti-Helmínticos/uso terapêutico
Doenças dos Bovinos/tratamento farmacológico
Proteínas na Dieta/administração & dosagem
Enteropatias Parasitárias/veterinária
Infecções por Nematoides/veterinária
Infecções por Trematódeos/veterinária
[Mh] Termos MeSH secundário: Animais
Camboja/epidemiologia
Bovinos
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/parasitologia
Suplementos Nutricionais
Fasciola/efeitos dos fármacos
Fasciolíase/tratamento farmacológico
Fasciolíase/veterinária
Feminino
Hematócrito/veterinária
Enteropatias Parasitárias/tratamento farmacológico
Enteropatias Parasitárias/epidemiologia
Masculino
Morbidade
Nematoides/efeitos dos fármacos
Infecções por Nematoides/tratamento farmacológico
Infecções por Nematoides/epidemiologia
Paramphistomatidae/efeitos dos fármacos
Infecções por Trematódeos/tratamento farmacológico
Infecções por Trematódeos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Dietary Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


  4 / 751 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28152102
[Au] Autor:Ullah R; Rehman A; Zafeer MF; Rehman L; Khan YA; Khan MA; Khan SN; Khan AU; Abidi SM
[Ad] Endereço:Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, India.
[Ti] Título:Anthelmintic Potential of Thymoquinone and Curcumin on Fasciola gigantica.
[So] Source:PLoS One;12(2):e0171267, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fasciolosis an economically important global disease of ruminants in the temperate and tropical regions, caused by Fasciola hepatica and F. gigantica, respectively, also poses a potential zoonotic threat. In India alone it causes huge losses to stakeholders. Anthelmintics including triclabendazole have been used to control this menace but the emerging resistance against the available compounds necessitates identification of novel and alternative therapeutic measures involving plant derived natural compounds for their anthelmintic potential. Thymoquinone (T) and curcumin (C), the active ingredients of Nigella sativa and Curcuma longa respectively have been used as antiparasitic agents but the information on their flukicidal effect is very limited. Adult flukes of F. gigantica were in vitro exposed to different concentrations of thymoquinone and curcumin separately for 3h at 37+ 1°C. A significant (p<0.05) reduction in the worm motility at 60 µM concentration of both T and C was observed though all the worms remained alive after 3h exposure, whereas the effect on egg shedding was statistically insignificant. Pronounced tegumental disruptions and erosion of spines in the posterior region and around the acetabulum was evident. A significant (p<0.05) decrease in glutathione-S-transferase and superoxide dismutase activity and reduced glutathione (GSH) level was observed, while protein carbonylation increased differentially. A significant inhibition of CathepsinL (CatL) gene expression in thymoquinone treated worms was also evident. Further, in silico molecular docking of T and C with CatL revealed a stronger interaction of curcumin with the involvement of higher number of amino acids as compared to thymoquinone that could be more effective in inhibiting the antioxidant enzymes of F. gigantica. It is concluded that both the compounds understudy will decrease the detoxification ability of F. gigantica, while inhibition of CatL will significantly affect their virulence potential. Thus, both thymoquinone and curcumin appeared to be promising anthelmintic compounds for further investigations.
[Mh] Termos MeSH primário: Antiplatelmínticos/farmacologia
Benzoquinonas/farmacologia
Curcumina/farmacologia
Fasciola/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Relação Dose-Resposta a Droga
Glutationa/metabolismo
Glutationa Transferase/metabolismo
Testes de Sensibilidade Parasitária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiplatyhelmintic Agents); 0 (Benzoquinones); 490-91-5 (thymoquinone); EC 2.5.1.18 (Glutathione Transferase); GAN16C9B8O (Glutathione); IT942ZTH98 (Curcumin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171267


  5 / 751 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28043381
[Au] Autor:Kueakhai P; Changklungmoa N; Waseewiwat P; Thanasinpaiboon T; Cheukamud W; Chaichanasak P; Sobhon P
[Ad] Endereço:Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Road, Mueang District, Chonburi 20131, Thailand.
[Ti] Título:Characterization and vaccine potential of Fasciola gigantica saposin-like protein 1 (SAP-1).
[So] Source:Vet Parasitol;233:115-122, 2017 Jan 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The recombinant Fasciola gigantica Saposin-like protien-1 (rFgSAP-1) was cloned by polymerase chain reaction (PCR) from NEJ cDNA, expressed in Escherichia coli BL21 (DE3) and used for production of a polyclonal antibody in rabbits (anti-rFgSAP-1). By immunoblotting and immunohistochemistry, rabbit IgG anti-rFgSAP-1 reacted with rFgSAP-1 at a molecular weight 12kDa, but not with rFgSAP-2. The rFgSAP-1 reacted with antisera from mouse infected with F. gigantica metacercariae collected at 2, 4, and 6 weeks after infection. The FgSAP-1 protein was expressed at a high level in the caecal epithelium of metacercariae and NEJs. The vaccination was performed in Imprinting Control Region (ICR) mice (n=10) by subcutaneous injection with 50µg of rFgSAP-1 combined with Alum adjuvant. Two weeks after the second boost, mice were infected with 15 metacercariae per mouse by the oral route. The percents protection of rFgSAP-1 vaccine were estimated to be 73.2% and 74.3% when compared with non vaccinated-infected and adjuvant-infected controls, respectively. The levels of IgG1 and IgG2a specific to rFgSAP-1 in the immune sera, which are indicative of Th2 and Th1 immune responses, were inversely and significantly correlated with the numbers of worm recoveries. The rFgSAP-1-vaccinated mice showed significantly reduced levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and liver damage. These indicated that rFgSAP-1 has strong potential as a vaccine candidate against F. gigantica, whose efficacy will be studied further in large economic animals including cattle, sheep, and goat.
[Mh] Termos MeSH primário: Fasciola/genética
Fasciolíase/imunologia
Fasciolíase/prevenção & controle
Proteínas de Helminto/imunologia
Proteínas Recombinantes/imunologia
Vacinas Sintéticas/imunologia
[Mh] Termos MeSH secundário: Alanina Transaminase/sangue
Animais
Anticorpos Anti-Helmínticos/sangue
Aspartato Aminotransferases/sangue
Escherichia coli/genética
Fasciola/imunologia
Fasciolíase/parasitologia
Proteínas de Helminto/genética
Fígado/patologia
Camundongos
Camundongos Endogâmicos ICR
Proteínas Recombinantes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (FHAP protein, Fasciola hepatica); 0 (Helminth Proteins); 0 (Recombinant Proteins); 0 (Vaccines, Synthetic); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE


  6 / 751 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27804894
[Au] Autor:Ichikawa-Seki M; Peng M; Hayashi K; Shoriki T; Mohanta UK; Shibahara T; Itagaki T
[Ad] Endereço:Laboratory of Veterinary Parasitology, Faculty of Agriculture,Iwate University,Ueda 3-18-8,Morioka 020-8550,Japan.
[Ti] Título:Nuclear and mitochondrial DNA analysis reveals that hybridization between Fasciola hepatica and Fasciola gigantica occurred in China.
[So] Source:Parasitology;144(2):206-213, 2017 Feb.
[Is] ISSN:1469-8161
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The well-known pathogens of fasciolosis, Fasciola hepatica (Fh) and Fasciola Gigantica (Fg), possess abundant mature sperms in their seminal vesicles, and thus, they reproduce bisexually. On the other hand, aspermic Fasciola flukes reported from Asian countries, which have no sperm in their seminal vesicles, probably reproduce parthenogenetically. The aim of this study was to reveal the origin of aspermic Fasciola flukes. The nuclear single copy markers, phosphoenolpyruvate carboxykinase and DNA polymerase delta, were employed for analysis of Fasciola species from China. The hybrid origin of aspermic Fasciola flukes was strongly suggested by the presence of the Fh/Fg type, which includes DNA fragments of both F. hepatica and F. gigantica. China can be regarded as the cradle of the interspecific hybridization because F. hepatica and F. gigantica were detected in the northern and southern parts of China, respectively, and hybrids flukes were distributed between the habitats of the two species. The Chinese origin was supported by the fact that a larger number of mitochondrial NADH dehydrogenase subunit 1 (nad1) haplotypes was detected in Chinese aspermic Fasciola populations than in aspermic populations from the neighbouring countries. Hereafter, 'aspermic' Fasciola flukes should be termed as 'hybrid' Fasciola flukes.
[Mh] Termos MeSH primário: DNA de Helmintos/genética
Fasciola/genética
[Mh] Termos MeSH secundário: Animais
China
DNA Mitocondrial/genética
Regulação da Expressão Gênica/fisiologia
Haplótipos
Proteínas de Helminto/genética
Proteínas de Helminto/metabolismo
Masculino
Glândulas Seminais
Especificidade da Espécie
Espermatogênese/genética
Espermatogênese/fisiologia
Espermatozoides
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth); 0 (DNA, Mitochondrial); 0 (Helminth Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161103
[St] Status:MEDLINE
[do] DOI:10.1017/S003118201600161X


  7 / 751 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27906040
[Au] Autor:Amer S; ElKhatam A; Zidan S; Feng Y; Xiao L
[Ad] Endereço:Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA, USA.
[Ti] Título:Identity of Fasciola spp. in sheep in Egypt.
[So] Source:Parasit Vectors;9(1):623, 2016 Dec 01.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In Egypt, liver flukes, Fasciola spp. (Digenea: Fasciolidae), have a serious impact on the farming industry and public health. Both Fasciola hepatica and Fasciola gigantica are known to occur in cattle, providing the opportunity for genetic recombination. Little is known on the identity and genetic variability of Fasciola populations in sheep. METHODS: This study was performed to determine the prevalence of liver flukes in sheep in Menofia Province as a representative area of the delta region in Egypt, as measured by postmortem examination of slaughtered animals at three abattoirs. The identity and genetic variability of Fasciola spp. in slaughtered animals were determined by PCR-sequence analysis of the nuclear ribosomal internal transcribed spacer 1 (ITS1) and the mitochondrial NADH dehydrogenase subunit 1 (nad1) genes. RESULTS: Physical inspection of the liver indicated that 302 of 2058 (14.7%) slaughtered sheep were infected with Fasciola spp. Sequence analysis of the ITS1 and nad1 genes of liver flukes from 17 animals revealed that 11 animals were infected with F. hepatica, four with F. gigantica, and two with both species. Seventy eight of 103 flukes genetically characterized from these animals were F. hepatica, 23 were F. gigantica, and two had ITS1 sequences identical to F. hepatica but nad1 sequences identical to F. gigantica. nad1 sequences of Egyptian isolates of F. gigantica showed pronounced differences from those in the GenBank database. Egyptian F. gigantica haplotypes formed haplogroup D, which clustered in a sister clade with haplogroups A, B and C circulating in Asia, indicating the existence of geographic isolation in the species. CONCLUSIONS: Both F. hepatica and F. gigantica are prevalent in sheep in Egypt and an introgressed form of the two occurs as the result of genetic recombination. In addition, a geographically isolated F. gigantica population is present in the country. The importance of these observations in epidemiology of fascioliasis needs to be examined in future studies.
[Mh] Termos MeSH primário: Fasciola/classificação
Fasciola/isolamento & purificação
Fasciolíase/veterinária
Variação Genética
Doenças dos Ovinos/epidemiologia
Doenças dos Ovinos/parasitologia
[Mh] Termos MeSH secundário: Animais
Análise por Conglomerados
DNA de Helmintos/química
DNA de Helmintos/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Egito/epidemiologia
Fasciola/genética
Fasciolíase/epidemiologia
Fasciolíase/parasitologia
Genótipo
Proteínas Mitocondriais/genética
Epidemiologia Molecular
NADH Desidrogenase/genética
Filogenia
Reação em Cadeia da Polimerase
Prevalência
Recombinação Genética
Análise de Sequência de DNA
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth); 0 (DNA, Ribosomal Spacer); 0 (Mitochondrial Proteins); EC 1.6.99.3 (NADH Dehydrogenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE


  8 / 751 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27890078
[Au] Autor:Lyngdoh D; Sharma S; Roy B; Tandon V
[Ad] Endereço:Department of Zoology, Northeastern Hill University, Shillong 793022, Meghalaya, India.
[Ti] Título:Animal Fascioliasis: Perspectives from high altitudinal regions.
[So] Source:Vet Parasitol;232:21-31, 2016 Dec 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The parasitic flukes of the genus Fasciola (Platyhelminthes: Trematoda: Digenea) cause fascioliasis or liver-rot disease in ruminant livestock in tropical and sub-tropical regions of the world. Classically, two species of Fasciola- F. hepatica and F. gigantica, are universally recognized as taxonomically valid species. Our survey studies on ovid and bovid animals including yak and mithun from high altitudinal mountainous regions in Northeast India revealed the occurrence of Fasciola gigantica and also Fasciola sp.- an intermediate form, at altitudes between 5000 and 14,085 feet above sea level (asl). Two morphotypes- F. hepatica - like and F. gigantica - like, of Fasciola species were reported from the high altitudinal areas of Northeast India; most of these locales constitute new-locality and first records for the occurrence of these liver flukes.
[Mh] Termos MeSH primário: Altitude
Doenças dos Bovinos/parasitologia
Fasciola/fisiologia
Fasciolíase/veterinária
[Mh] Termos MeSH secundário: Distribuição Animal
Animais
Bovinos
Doenças dos Bovinos/epidemiologia
Fasciola hepatica/fisiologia
Fasciolíase/epidemiologia
Fasciolíase/parasitologia
Índia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170112
[Lr] Data última revisão:
170112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161129
[St] Status:MEDLINE


  9 / 751 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27884440
[Au] Autor:Greter H; Batil AA; Alfaroukh IO; Grimm F; Ngandolo BN; Keiser J; Utzinger J; Zinsstag J; Hattendorf J
[Ad] Endereço:Swiss Tropical and Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland.
[Ti] Título:Re-infection with Fasciola gigantica 6-month post-treatment with triclabendazole in cattle from mobile pastoralist husbandry systems at Lake Chad.
[So] Source:Vet Parasitol;230:43-48, 2016 Oct 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:At Lake Chad in central Africa, livestock fascioliasis caused by Fasciola gigantica represents a major veterinary health problem, particularly in cattle reared in mobile pastoralist husbandry systems. We assessed re-infection after a single dose of triclabendazole with fascioliasis in cattle in a mobile pastoralist setting towards the end of the dry season. Within the cattle herds of 14 groups of mobile pastoralists, 375 cattle were randomly selected. A faecal sample was obtained from each animal to determine the prevalence of F. gigantica. Animals were administered a single oral dose of triclabendazole (12mg/kg). A second faecal sample was obtained 6-month post-treatment after cattle had returned from the annual migration cycle. Faecal samples were fixed in sodium acetate-acetic acid-formalin (SAF), and examined for F. gigantica using the sedimentation technique. From the 375 cattle enrolled at baseline, 198 animals (53%) in 12 groups of mobile pastoralists were re-sampled at the 6-month follow-up. Baseline prevalence did not differ noteworthy between animals lost to follow-up and those re-examined. At baseline, bovine fascioliasis prevalence in cattle with follow-up data was 41.9% (95% confidence interval (CI) 35.2-48.9%). At the 6-month post-treatment follow-up, the prevalence was 46.0% (95% CI 39.2-52.9%), ranging between 0% and 75% at the herd level. The mean faecal egg counts at the unit of the herd were higher at follow-up compared to baseline. The observed persistent high prevalence of F. gigantica infection in cattle shows that a single pre-rainy season treatment does not prevent rapid re-infection despite the partial migration away from the high-risk areas at Lake Chad into drier areas. A locally adapted strategic control package for fascioliasis in cattle in the Lake Chad area ought to integrate targeted triclabendazole treatment and seasonal transhumance practices.
[Mh] Termos MeSH primário: Anti-Helmínticos/uso terapêutico
Benzimidazóis/uso terapêutico
Doenças dos Bovinos/tratamento farmacológico
Fasciolíase/tratamento farmacológico
Fasciolíase/veterinária
[Mh] Termos MeSH secundário: Criação de Animais Domésticos
Animais
Bovinos
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/prevenção & controle
Chade
Fasciola/fisiologia
Fasciolíase/epidemiologia
Fasciolíase/prevenção & controle
Fezes/parasitologia
Lagos
Prevalência
Recidiva
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Benzimidazoles); 4784C8E03O (triclabendazole)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE


  10 / 751 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27514897
[Au] Autor:Changklungmoa N; Phoinok N; Yencham C; Sobhon P; Kueakhai P
[Ad] Endereço:Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Road, Mueang District, Chonburi 20131, Thailand.
[Ti] Título:Vaccine potential of recombinant cathepsinL1G against Fasciola gigantica in mice.
[So] Source:Vet Parasitol;226:124-31, 2016 Aug 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, we characterized and investigated the vaccine potential of FgCatL1G against Fasciola gigantica infection in mice. Recombinant mature FgCatL1G (rmFgCatL1G) was expressed in Escherichia coli BL21. The vaccination was performed in Imprinting Control Region (ICR) mice (n=10) by subcutaneous injection with 50µg of rmFgCatL1G combined with Freund's adjuvant. Two weeks after the second boost, mice were infected with 15 metacercariae by the oral route. The percents of protection of rmFgCatL1G vaccine were estimated to be 56.5% and 58.3% when compared with non vaccinated-infected and adjuvant-infected controls, respectively. Antibodies in the immune sera of vaccinated mice were shown by immunoblot to react with the native FgCatL1s in the extract of all stages of parasites and rmFgCatL1H, recombinant pro - FgCatL1 (rpFgCatL1). By immunohistochemistry, the immune sera also reacted with FgCatL1s in the caecal epithelial cells of the parasites. The levels of IgG1 and IgG2a in the immune sera, which are indicative of Th2 and Th1 immune responses, were also increased with IgG1 predominating. The levels of serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) in rmFgCatL1G-immunized group showed no significant difference from the control groups, but pathological lesions of livers in rmFgCatL1G-immunized group showed significant decrease when compared to the control groups. This study indicates that rmFgCatL1G has a vaccine potential against F. gigantica in mice, and this potential will be tested in larger livestock animals.
[Mh] Termos MeSH primário: Catepsinas/imunologia
Cisteína Endopeptidases/imunologia
Fasciola/imunologia
Fasciolíase/prevenção & controle
Proteínas de Helminto/imunologia
Vacinas
[Mh] Termos MeSH secundário: Alanina Transaminase/sangue
Animais
Anticorpos Anti-Helmínticos/sangue
Aspartato Aminotransferases/sangue
Bovinos
Cricetinae
Eletroforese em Gel de Poliacrilamida
Ensaio de Imunoadsorção Enzimática
Fasciolíase/patologia
Immunoblotting
Imunoglobulina G/sangue
Fígado/patologia
Lymnaea/parasitologia
Masculino
Mesocricetus
Camundongos
Camundongos Endogâmicos ICR
Vacinas Sintéticas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Helminth Proteins); 0 (Immunoglobulin G); 0 (Vaccines); 0 (Vaccines, Synthetic); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase); EC 3.4.- (Cathepsins); EC 3.4.22 (cat-L1B protein, Fasciola gigantica); EC 3.4.22.- (Cysteine Endopeptidases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160813
[St] Status:MEDLINE



página 1 de 76 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde