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[PMID]: 28285504 [Au] Autor: Wongsawad C; Wongsawad P; Sukontason K; Maneepitaksanti W; Nantarat N [Ad] Endereço: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. [Ti] Título: Molecular Phylogenetics of (Digenea: Heterophyidae) Originated from Freshwater Fish from Chiang Mai Province, Thailand. [So] Source: Korean J Parasitol;55(1):31-37, 2017 Feb. [Is] ISSN: 1738-0006 [Cp] País de publicação: Korea (South) [La] Idioma: eng [Ab] Resumo: This study aimed to investigate the morphology and reconstruct the phylogenetic relationships of originating from 5 species of freshwater fish, i.e., , and , in Chiang Mai province, Thailand. Sequence-related amplified polymorphism (SRAP) and phylogeny based on internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were performed. The results showed similar morphologies of adult from day 5 after infection in chicks. originated from 4 species of freshwater fish had the same number of circumoral spines on the oral sucker, except for those from which revealed 34 circumoral spines. The phylogenetic tree obtained from SRAP profile and the combination of ITS2 and CO1 sequence showed similar results that were correlated with the number of circumoral spines in adult worms. Genetic variability of also occurred in different species of freshwater fish hosts. However, more details of adult worm morphologies and more sensitive genetic markers are needed to confirm the species validity of with 34 circumoral spines originating from in the future. [Mh] Termos MeSH primário: Doenças dos Peixes/parasitologia Água Doce Heterophyidae/classificação Heterophyidae/genética Filogenia Infecções por Trematódeos/veterinária [Mh] Termos MeSH secundário: Animais Análise por Conglomerados DNA de Helmintos/química DNA de Helmintos/genética DNA Espaçador Ribossômico/química DNA Espaçador Ribossômico/genética Complexo IV da Cadeia de Transporte de Elétrons/genética Peixes Heterophyidae/anatomia & histologia Heterophyidae/isolamento & purificação Microscopia Análise de Sequência de DNA Tailândia Infecções por Trematódeos/parasitologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Helminth); 0 (DNA, Ribosomal Spacer); EC 1.9.3.1 (Electron Transport Complex IV) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170414 [Lr] Data última revisão: 170414 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170314 [St] Status: MEDLINE [do] DOI: 10.3347/kjp.2017.55.1.31

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[PMID]: 28069063 [Au] Autor: Le TH; Nguyen KT; Nguyen NT; Doan HT; Dung DT; Blair D [Ad] Endereço: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18. Hoang Quoc Viet Rd,, Cau Giay, Hanoi, Vietnam. imibtvn@gmail.com. [Ti] Título: The ribosomal transcription units of Haplorchis pumilio and H. taichui and the use of 28S rDNA sequences for phylogenetic identification of common heterophyids in Vietnam. [So] Source: Parasit Vectors;10(1):17, 2017 Jan 09. [Is] ISSN: 1756-3305 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Heterophyidiasis is now a major public health threat in many tropical countries. Species in the trematode family Heterophyidae infecting humans include Centrocestus formosanus, Haplorchis pumilio, H. taichui, H. yokogawai, Procerovum varium and Stellantchasmus falcatus. For molecular phylogenetic and systematic studies on trematodes, we need more prospective markers for taxonomic identification and classification. This study provides near-complete ribosomal transcription units (rTU) from Haplorchis pumilio and H. taichui and demonstrates the use of 28S rDNA sequences for identification and phylogenetic analysis. RESULTS: The near-complete ribosomal transcription units (rTU), consisting of 18S, ITS1, 5.8S, ITS2 and 28S rRNA genes and spacers, from H. pumilio and H. taichui from human hosts in Vietnam, were determined and annotated. Sequence analysis revealed tandem repetitive elements in ITS1 in H. pumilio and in ITS2 in H. taichui. A phylogenetic tree inferred from 28S rDNA sequences of 40 trematode strains/species, including 14 Vietnamese heterophyid individuals, clearly confirmed the status of each of the Vietnamese species: Centrocestus formosanus, Haplorchis pumilio, H. taichui, H. yokogawai, Procerovum varium and Stellantchasmus falcatus. However, the family Heterophyidae was clearly not monophyletic, with some genera apparently allied with other families within the superfamily Opisthorchioidea (i.e. Cryptogonimidae and Opisthorchiidae). These families and their constituent genera require substantial re-evaluation using a combination of morphological and molecular data. Our new molecular data will assist in such studies. CONCLUSIONS: The 28S rDNA sequences are conserved among individuals within a species but varied between genera. Based on analysis of 40 28S rDNA sequences representing 19 species in the superfamily Opisthorchioidea and an outgroup taxon (Alaria alata, family Diplostomidae), six common human pathogenic heterophyids were identified and clearly resolved. The phylogenetic tree inferred from these sequences again confirmed anomalies in molecular placement of some members of the family Heterophyidae and demonstrates the need for reappraisal of the entire superfamily Opisthorchioidea. The new sequences provided here supplement those already available in public databases and add to the array of molecular tools that can be used for the diagnosis of heterophyid species in human and animal infections. [Mh] Termos MeSH primário: DNA de Helmintos/genética DNA Espaçador Ribossômico/genética Doenças dos Peixes/parasitologia Heterophyidae/isolamento & purificação Filogenia RNA Ribossômico 28S/genética Infecções por Trematódeos/parasitologia [Mh] Termos MeSH secundário: Animais Feminino Peixes Heterophyidae/classificação Heterophyidae/genética Masculino Vietnã [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Helminth); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 28S) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170515 [Lr] Data última revisão: 170515 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170111 [St] Status: MEDLINE [do] DOI: 10.1186/s13071-017-1968-0

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[PMID]: 27914977 [Au] Autor: Garcia-Vedrenne AE; Quintana AC; DeRogatis AM; Dover CM; Lopez M; Kuris AM; Hechinger RF [Ad] Endereço: Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA 93106, USA. Electronic address: vedrenne@lifesci.ucsb.edu. [Ti] Título: Trematodes with a reproductive division of labour: heterophyids also have a soldier caste and early infections reveal how colonies become structured. [So] Source: Int J Parasitol;47(1):41-50, 2017 Jan. [Is] ISSN: 1879-0135 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Recent findings have extended the documentation of complex sociality to the Platyhelminthes, describing the existence of a reproductive division of labour involving a soldier caste among the parthenitae of trematode parasites. However, all species examined to date occupy high positions in trematode interspecific dominance hierarchies and belong to two closely related families, the Echinostomatidae and the Philophthalmidae (Superfamily Echinostomatoidea). Further, the two species documented as lacking soldiers also belong to the Echinostomatidae. Here, we examine four species of intermediate dominance, all belonging to the family Heterophyidae (Superfamily Opisthorchioidea): Euhaplorchis californiensis, Phocitremoides ovale, Pygidiopsoides spindalis and Stictodora hancocki, all of which infect the California horn snail, Cerithideopsis californica (=Cerithidea californica). We quantify morphology, distribution and behaviour of rediae from fully developed colonies. We also provide information on colony structure for three developing heterophyid colonies to better understand colony development. We discuss the implications of our findings, particularly with respect to how they suggest alternatives to the conclusions of other researchers concerning the nature of trematode sociality. Our analyses of morphological, distributional and behavioural patterns of developed colonies indicate that these heterophyid trematodes have a non-reproductive caste whose function is defence of the colony from invading trematodes. Hence, a soldier caste occurs for species lower in dominance hierarchies than previously known, and is present in at least two superfamilies of digenean trematodes, suggesting that selection for a soldier caste may be much more common among the Trematoda than previously recognised. [Mh] Termos MeSH primário: Trematódeos/fisiologia [Mh] Termos MeSH secundário: Animais Comportamento Animal Biodiversidade California Echinostomatidae/anatomia & histologia Echinostomatidae/classificação Echinostomatidae/fisiologia Heterophyidae/anatomia & histologia Heterophyidae/classificação Heterophyidae/fisiologia Reprodução/fisiologia Caramujos/parasitologia Comportamento Social Especificidade da Espécie Trematódeos/patogenicidade Infecções por Trematódeos/parasitologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170731 [Lr] Data última revisão: 170731 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161205 [St] Status: MEDLINE

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[PMID]: 27882434 [Au] Autor: Manpratum Y; Kaewkes W; Echaubard P; Sripa B; Kaewkes S [Ad] Endereço: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. [Ti] Título: New locality record for Haplorchoides mehrai and possible interactions with Opisthorchis viverrini metacercariae in cyprinid fishes in Northeast Thailand. [So] Source: Parasitol Res;116(2):601-608, 2017 Feb. [Is] ISSN: 1432-1955 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Metacercariae of Opisthorchis viverrini, a carcinogenic liver fluke, and Haplorchoides sp., a trematode maturing in catfish, are commonly found in cyprinid fish, the second intermediate hosts of both flukes. However, the specific identity of Haplorchoides sp. in Thailand and a precise assessment of the effects of co-infections with O. viverrini have never been clarified. Therefore, we aimed to identify the species of Haplorchoides and to investigate possible interactions of the two trematode species in cyprinid fishes. Based on the morphology and morphometry of the cercaria, metacercaria, and adult stages, the Haplorchoides species found was identified as Haplorchoides mehrai Pande and Shukla 1976. Thailand is formally recorded as a new locality for H. mehrai, where naturally infected hosts include the snail Melanoides tuberculata (first intermediate host), the cyprinid fishes Hampala dispar, Cyclocheilichthys apogon, Puntius leiacanthus, Labiobarbus burmanicus, and Cirrhina jullieni (second intermediate hosts), and a catfish, Mystus nemurus (definitive host). The co-infection rates of O. viverrini and H. mehrai were significantly associated with fish species and fish body region (P < 0.001), with an overall significantly higher average intensity of H. mehrai (126.26 metacercariae/fish) than that of O. viverrini (18.02 metacercariae/fish). Further work is required to demonstrate the extent and mechanisms of possible interactions between these trematode species in the fish host. These data may provide a better understanding of O. viverrini transmission dynamics, and help design integrated control interventions. [Mh] Termos MeSH primário: Doenças dos Peixes/parasitologia Heterophyidae/isolamento & purificação Heterophyidae/fisiologia Opistorquíase/veterinária Opisthorchis/fisiologia Infecções por Trematódeos/veterinária [Mh] Termos MeSH secundário: Animais Peixes-Gato/parasitologia Coinfecção/parasitologia Cyprinidae/parasitologia Heterophyidae/genética Opistorquíase/parasitologia Opisthorchis/genética Opisthorchis/isolamento & purificação Tailândia Infecções por Trematódeos/parasitologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 171007 [Lr] Data última revisão: 171007 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161125 [St] Status: MEDLINE [do] DOI: 10.1007/s00436-016-5324-7

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[PMID]: 27836745 [Au] Autor: Shumenko PG; Tatonova YV; Besprozvannykh VV [Ad] Endereço: Institute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Sciences, 100-letiya Street, 159, Vladivostok 690022, Russia. [Ti] Título: Metagonimus suifunensis sp. n. (Trematoda: Heterophyidae) from the Russian Southern Far East: Morphology, life cycle, and molecular data. [So] Source: Parasitol Int;66(1):982-991, 2017 Feb. [Is] ISSN: 1873-0329 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: The trematode from the Russian Southern Far East, earlier defined as M. yokogawai Katsuradai, 1912, was determined as a representative of the new species Metagonimus suifunensis sp. n. The life cycle of this trematode was experimentally studied and morphometric data for the cercariae, metacercariae and adult worm stages were obtained. The molecular data for samples from six Russian localities were received using the ITS1-5.8S-ITS2 region and 28S gene nuclear ribosomal DNA. The results revealed that M. suifunensis sp. n. does not have significant morphometric differences with M. yokogawai, M. takahashii and M. miyatai. However, the new species validity was confirmed by the molecular data. The phylogenetic relationship analysis of Metagonimus representatives showed that M. suifunensis sp. n. is well-differentiated specie with low variability in the whole area. [Mh] Termos MeSH primário: Doenças dos Peixes/parasitologia Heterophyidae/anatomia & histologia Heterophyidae/genética Estágios do Ciclo de Vida Infecções por Trematódeos/veterinária [Mh] Termos MeSH secundário: Animais DNA Espaçador Ribossômico/genética Peixes/parasitologia Heterophyidae/classificação Heterophyidae/crescimento & desenvolvimento Filogenia RNA Ribossômico 28S/genética Federação Russa Caramujos/parasitologia Infecções por Trematódeos/parasitologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 28S) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170417 [Lr] Data última revisão: 170417 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161113 [St] Status: MEDLINE

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[PMID]: 27260384 [Au] Autor: Borges JN; Costa VS; Mantovani C; Barros E; Santos EG; Mafra CL; Santos CP [Ad] Endereço: Laboratório de Avaliação e Promoção da Saúde Ambiental (LAPSA), Fiocruz, Rio de Janeiro, Brazil. [Ti] Título: Molecular characterization and confocal laser scanning microscopic study of Pygidiopsis macrostomum (Trematoda: Heterophyidae) parasites of guppies Poecilia vivipara. [So] Source: J Fish Dis;40(2):191-203, 2017 Feb. [Is] ISSN: 1365-2761 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Pygidiopsis macrostomum and Ascocotyle (Phagicola) pindoramensis (Digenea: Heterophyidae) parasitize guppies as intermediate hosts and, respectively, fish-eating mammals or birds as definitive hosts. Heterophyids have zoonotic potential, and molecular studies associated with morphological and ecological aspects have helped to clarify their taxonomy and phylogeny. Poecilia vivipara naturally parasitized by metacercariae of both species (100% prevalence) exhibit no external signs of parasitism. In this work, four new sequences of P. macrostomum (18S rDNA, 28S rDNA and ITS2 rDNA) and one new sequence of A. (P.) pindoramensis (mtDNA cox-1) are presented. Phylogeny reconstructions linked P. macrostomum to other heterophyids, but the separation of the Heterophyidae and Opisthorchiidae remains unclear. Additionally, we used indirect immunocytochemistry and the phalloidin-fluorescence techniques allied with confocal laser scanning microscopy to describe muscular and neuronal structures of P. macrostomum. A complex arrangement of muscular fibres is associated with the tegument, suckers, gut and reproductive system. Radial fibres around the ventral sucker are thick, branched and extend to the body wall. High-resolution confocal imaging revealed a typical digenean muscular arrangement and important heterophyid morphological traits. These data will support future control measures to reduce the parasitism in guppies reared in fish farming systems, especially for aquarium and experimental purposes. [Mh] Termos MeSH primário: DNA de Helmintos/genética DNA Ribossômico/genética Doenças dos Peixes/epidemiologia Heterophyidae/fisiologia Poecilia Infecções por Trematódeos/veterinária [Mh] Termos MeSH secundário: Animais Brasil/epidemiologia Feminino Doenças dos Peixes/parasitologia Heterophyidae/anatomia & histologia Heterophyidae/genética Masculino Microscopia Confocal/veterinária Filogenia Prevalência Análise de Sequência de DNA/veterinária Infecções por Trematódeos/epidemiologia Infecções por Trematódeos/parasitologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Helminth); 0 (DNA, Ribosomal) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170519 [Lr] Data última revisão: 170519 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160605 [St] Status: MEDLINE [do] DOI: 10.1111/jfd.12504

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[PMID]: 27658608 [Au] Autor: Chai JY; Sohn WM; Na BK; Jeoung HG; Sinuon M; Socheat D [Ad] Endereço: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Korea. [Ti] Título: Stellantchasmus falcatus (Digenea: Heterophyidae) in Cambodia: Discovery of Metacercariae in Mullets and Recovery of Adult Flukes in an Experimental Hamster. [So] Source: Korean J Parasitol;54(4):537-41, 2016 Aug. [Is] ISSN: 1738-0006 [Cp] País de publicação: Korea (South) [La] Idioma: eng [Ab] Resumo: Stellantchasmus falcatus (Digenea: Heterophyidae) is first reported from Cambodia through recovery of the metacercariae from mullet fish and adult flukes from an experimentally infected hamster. We purchased 7 mullets, Chelon macrolepis, in a local market of Phnom Penh, Cambodia, and each of them was examined by the artificial digestion method on May 2010. The metacercariae of S. falcatus were detected in all mullets (100%) examined, and their average density was 177 per fish. They were elliptical, 220×168 µm in average size. They were orally infected to an hamster to obtain adult flukes. Adults recovered at day 10 post infection were observed with a light microscope and a scanning electron microscope (SEM). They were small, 450×237 µm in average size, had a small oral sucker (41×50 µm), subglobular pharynx (29×21 µm), slender esophagus (57 µm), long and thick-walled expulsor (119×32 µm), spherical ovary (58×69 µm), and 2 ovoid testes (right: 117×74 µm; left: 114×63 µm). Eggs were small, yellow, and 23×12 µm in average size. In SEM observations, tegumental spines were densely distributed on the whole tegument, and single small type I sensory papillae were distributed around the lip of oral sucker. The small ventral sucker was dextrally located and had 8 type I sensory papillae on the left margin. It has been first confirmed in the present study that the mullet, C. macrolepis, is playing the role of a second intermediate host of S. falcatus in Cambodia. [Mh] Termos MeSH primário: Heterophyidae/isolamento & purificação Metacercárias/isolamento & purificação Smegmamorpha Infecções por Trematódeos/veterinária [Mh] Termos MeSH secundário: Experimentação Animal Animais Camboja Cricetinae Heterophyidae/anatomia & histologia Heterophyidae/crescimento & desenvolvimento Metacercárias/anatomia & histologia Metacercárias/crescimento & desenvolvimento Microscopia Carga Parasitária Infecções por Trematódeos/parasitologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170404 [Lr] Data última revisão: 170404 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160924 [St] Status: MEDLINE [do] DOI: 10.3347/kjp.2016.54.4.537

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[PMID]: 27658602 [Au] Autor: Sohn WM; Na BK; Cho SH; Lee WJ; Park MY; Lee SW; Choi SB; Huh BN; Seok WS [Ad] Endereço: Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 52828, Korea. [Ti] Título: Pygidiopsis summa (Digenea: Heterophyidae): Status of Metacercarial Infection in Mullets from Coastal Areas in the Republic of Korea. [So] Source: Korean J Parasitol;54(4):497-502, 2016 Aug. [Is] ISSN: 1738-0006 [Cp] País de publicação: Korea (South) [La] Idioma: eng [Ab] Resumo: To know the infection status of zoonotic trematode metacercariae in brackish water fish, we surveyed mullets collected from 18 coastal areas in the Republic of Korea. The metacercariae of Pygidiopsis summa were detected in 236 (68.2%) out of 346 mullets examined. They were found in mullets from 15 areas except for those from Boseong-gun (Jeollanam-do), Pohang-si, and Uljin-gun (Gyeongsangbuk-do). Especially in mullets from Taean-gun (Chungcheongnam-do) and Geoje-si (Gyeongsangnam-do), their prevalences were 100% and 95.5%, and the average metacercarial density was more than 1,000 per fish. They were also detected in mullets from 3 coastal lakes, Gyeongpoho, Songjiho, and Hwajinpoho, in Gangwon-do, and their average densities were 419, 147, and 672 per infected fish, respectively. The metacercariae of 5 other heterophyid species, including Heterophyes nocens, Heterophyopsis continua, Metagonimus sp., Stictodora fuscata, and Stictodora lari, were found in the mullets examined. The metacercariae of H. nocens were detected in 66.7, 100, 28.6, 81.6, 3.9, 61.5, and 27.3% of mullets from Muan-gun, Shinan-gun, Haenam-gun, Gangjin-gun, and Boseong-gun (Jeollanam-do), Hadong-gun, and Geoje-si (Gyeongsangnam-do), and their metacercarial intensities were 64, 84, 119, 99, 1, 24, and 24 per fish infected, respectively. From the above results, it has been confirmed that P. summa metacercariae are heavily infected in mullets from coastal areas of Korea. It is suggested that residents who frequently consume raw mullet dish can be easily infected with heterophyid flukes. [Mh] Termos MeSH primário: Doenças dos Peixes/epidemiologia Doenças dos Peixes/parasitologia Heterophyidae/isolamento & purificação Metacercárias/isolamento & purificação Smegmamorpha Infecções por Trematódeos/veterinária [Mh] Termos MeSH secundário: Animais Heterophyidae/classificação Carga Parasitária Prevalência República da Coreia/epidemiologia Infecções por Trematódeos/epidemiologia Infecções por Trematódeos/parasitologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170404 [Lr] Data última revisão: 170404 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160924 [St] Status: MEDLINE [do] DOI: 10.3347/kjp.2016.54.4.497

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[PMID]: 27539726 [Au] Autor: Rosser TG; Baumgartner WA; Alberson NR; Woodyard ET; Reichley SR; Wise DJ; Pote LM; Griffin MJ [Ad] Endereço: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39762, USA. [Ti] Título: Austrodiplostomum sp., Bolbophorus sp. (Digenea: Diplostomidae), and Clinostomum marginatum (Digenea: Clinostomidae) metacercariae in inland silverside Menidia beryllina from catfish aquaculture ponds, with notes on the infectivity of Austrodiplostomum sp. cercariae in channel catfish Ictalurus punctatus. [So] Source: Parasitol Res;115(11):4365-4378, 2016 Nov. [Is] ISSN: 1432-1955 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: In the southeastern USA, catfish aquaculture is burdened by predation from piscivorous birds and the digenetic trematodes they carry. In addition to cultured ictalurid fish, other forage or incidental fish species inhabit catfish production ponds. Of these, the inland silverside Menidia beryllina was recently found to harbor larval metacercariae of several trematode species. Three species of metacercariae were reported, two of which represent the first morphological descriptions of an Austrodiplostomum sp. and Bolbophorus sp. metacercaria, respectively. A total of 15 silversides were collected from a commercial catfish pond and examined for trematode infection. These fish were parasitized by metacercariae of an Austrodiplostomum sp. (100 % prevalence) in the eyes and brain, a Bolbophorus sp. (86.7 % prevalence) in the musculature, and Clinostomum marginatum (33.3 % prevalence) in the musculature and fins. All three trematode species were characterized morphologically and molecularly by sequencing of the cytochrome c oxidase subunit 1 gene (CO1). In addition, the internal transcribed spacer 1 region (ITS1), 5.8S rRNA gene, and ITS2 region were determined for the Bolbophorus sp., which linked this metacercaria to a Bolbophorus sp. cercaria from a planorbid snail Planorbella trivolvis and an unnamed Bolbophorus sp. adult from the American white pelican Pelecanus erythrorhynchos. Furthermore, Biomphalaria havanensis snails were collected from the same pond and found actively shedding cercariae morphologically and molecularly consistent with a diplostomid cercaria reported from Bi . havanensis in catfish ponds in Mississippi, USA. Sequence comparisons deemed these cercariae conspecific to the Austrodiplostomum sp. from inland silverside described here. Channel catfish fingerlings were exposed to these cercariae at doses of 50 and 100 cercariae per fish. The infectivity of this Austrodiplostomum sp. in channel catfish was assessed at 10 and 20 days post exposure (dpe). Metacercariae were observed in both the eyes and brain of infected channel catfish, supporting molecular data that suggests the cercaria and metacercaria are both stages of a previously unidentified Austrodiplostomum sp. life cycle. [Mh] Termos MeSH primário: Doenças dos Peixes/parasitologia Ictaluridae/parasitologia Trematódeos Infecções por Trematódeos/veterinária [Mh] Termos MeSH secundário: Animais Aquicultura Aves/parasitologia Heterophyidae Larva Estágios do Ciclo de Vida Metacercárias/classificação Metacercárias/isolamento & purificação Tanques Smegmamorpha/parasitologia Caramujos Trematódeos/classificação Trematódeos/patogenicidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 171007 [Lr] Data última revisão: 171007 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160820 [St] Status: MEDLINE

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[PMID]: 27154766 [Au] Autor: Masala S; Piras MC; Sanna D; Chai JY; Jung BK; Sohn WM; Garippa G; Merella P [Ad] Endereço: Dipartimento di Medicina Veterinaria, Università di Sassari, via Vienna, 2, 07100, Sassari, Italy. [Ti] Título: Epidemiological and molecular data on heterophyid trematode metacercariae found in the muscle of grey mullets (Osteichthyes: Mugilidae) from Sardinia (western Mediterranean Sea). [So] Source: Parasitol Res;115(9):3409-17, 2016 Sep. [Is] ISSN: 1432-1955 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: This study is a contribution to the molecular taxonomy and epidemiology of heterophyid (Trematoda: Heterophyidae) metacercariae found in the muscle of Mugilidae (Osteichthyes) from Sardinia (western Mediterranean Sea). Sixty specimens of mugilids (13 Chelon labrosus, 18 Liza aurata, 6 Liza ramada, 8 Liza saliens, 15 Mugil cephalus) were examined and 17,899 metacercariae isolated in 95 % of the hosts. Four types of metacercariae were identified: Heterophyes sp. (n = 14,113), Heterophyes sp. -small (1225), Stictodora sp. (1606), and Ascocotyle (Phagicola) sp. (955). The experimental infection of a hamster with Heterophyes sp. metacercariae produced six adults identified as Heterophyes heterophyes and two as Heterophyes cf. nocens. The morphology of Heterophyes sp. -small metacercariae matched with that of Heterophyes dispar. The sequence analysis of the ITS2 and 28S portions of rDNA confirmed the morphological identification of metacercariae, showing four clusters. All adults grouped together with the Heterophyes sp. metacercariae, whereas adults of Heterophyes nocens from Korea clustered separately, showing that this species is distinguished from H. heterophyes, and suggesting caution in the exclusive use of the number of rodlets of the genital sucker to separate the two species. The presence of metacercariae was high in all hosts; the highest prevalence is of Heterophyes sp. (prevalence ≥78 %; mean intensity ≥135 metacercariae/100 g muscle), and the most heavily infected host is M. cephalus (prevalence = 100 %; mean intensity = 841 metacercariae/100 g muscle). [Mh] Termos MeSH primário: Doenças dos Peixes/parasitologia Heterophyidae/isolamento & purificação Metacercárias/isolamento & purificação Músculo Esquelético/parasitologia Smegmamorpha/parasitologia Infecções por Trematódeos/veterinária [Mh] Termos MeSH secundário: Animais Cricetinae Feminino Heterophyidae/classificação Heterophyidae/genética Heterophyidae/crescimento & desenvolvimento Itália Masculino Mar Mediterrâneo Metacercárias/classificação Metacercárias/genética Metacercárias/crescimento & desenvolvimento Prevalência Infecções por Trematódeos/epidemiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 171007 [Lr] Data última revisão: 171007 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160508 [St] Status: MEDLINE [do] DOI: 10.1007/s00436-016-5101-7

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