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[PMID]:28991924
[Au] Autor:Rahman SMM; Song HB; Jin Y; Oh JK; Lim MK; Hong ST; Choi MH
[Ad] Endereço:Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Korea.
[Ti] Título:Application of a loop-mediated isothermal amplification (LAMP) assay targeting cox1 gene for the detection of Clonorchis sinensis in human fecal samples.
[So] Source:PLoS Negl Trop Dis;11(10):e0005995, 2017 Oct.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Clonorchiasis is prevalent in the Far East, and a major health problem in endemic areas. Infected persons may experience, if not treated, serious complications such as bile stone formation, pyogenic cholangitis, and even cholangiocarcinoma. Early diagnosis and treatment are important to prevent serious complications and, therefore, the simple and reliable diagnostic method is necessary to control clonorchiasis in endemic areas, where resources for the diagnosis are limited. METHODOLOGY/PRINCIPLE FINDINGS: The loop-mediated isothermal amplification (LAMP) assay has been applied for the detection of Clonorchis sinensis DNA. Six primers targeting eight locations on the cytochrome c oxidase subunit 1 gene of C. sinensis were designed for species-specific amplification using the LAMP assay. The LAMP assay was sensitive enough to detect as little as 100 fg of C. sinensis genomic DNA and the detection limit in 100 mg of stool was as low as one egg. The assay was highly specific because no cross-reactivity was observed with the DNA of other helminths, protozoa or Escherichia coli. Then, LAMP assay was applied to human fecal samples collected from an endemic area of clonorchiasis in Korea. Using samples showing consistent results by both Kato-Katz method and real-time PCR as reference standards, the LAMP assay showed 97.1% (95% CI, 90.1-99.2) of sensitivity and 100% (95% CI, 92.9-100) of specificity. In stool samples with more than 100 eggs per gram of feces, the sensitivity achieved 100%. CONCLUSIONS: To detect C. sinensis in human fecal samples, the LAMP assay was applied and achieved high sensitivity and specificity. The LAMP assay can be utilized in field laboratories as a powerful tool for diagnosis and epidemiological survey of clonorchiasis.
[Mh] Termos MeSH primário: Clonorquíase/diagnóstico
Clonorchis sinensis/isolamento & purificação
Complexo IV da Cadeia de Transporte de Elétrons/química
Técnicas de Amplificação de Ácido Nucleico/métodos
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Clonorquíase/parasitologia
Clonorchis sinensis/genética
Complexo IV da Cadeia de Transporte de Elétrons/genética
Fezes/parasitologia
Proteínas de Helminto
Seres Humanos
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Helminth Proteins); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005995


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[PMID]:28764657
[Au] Autor:Han S; Tang Q; Chen R; Li Y; Shu J; Zhang X
[Ad] Endereço:Department of Parasitology, Harbin Medical University, Harbin, 150081, China.
[Ti] Título:Hepatic iron overload is associated with hepatocyte apoptosis during Clonorchis sinensis infection.
[So] Source:BMC Infect Dis;17(1):531, 2017 Aug 01.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hepatic iron overload has been implicated in many liver diseases; however, whether it is involved in clonorchiasis remains unknown. The purpose of this study is to investigate whether Clonorchis sinensis (C. sinensis) infection causes hepatic iron overload, analyze the relationship between the iron overload and associated cell apoptosis, so as to determine the role of excess iron plays in C. sinensis-induced liver injury. METHODS: The Perls' Prussian staining and atomic absorption spectrometry methods were used to investigate the iron overload in hepatic sections of wistar rats and patients infected with C. sinensis. The hepatic apoptosis was detected by transferase uridyl nick end labeling (TUNEL) methods. Spearman analysis was used for determining the correlation of the histological hepatic iron index and the apoptotic index. RESULTS: Blue iron particles were deposited mainly in the hepatocytes, Kupffer cells and endothelial cells, around the liver portal and central vein area of both patients and rats. The total iron score was found to be higher in the infected groups than the respective control from 8 weeks. The hepatic iron concentration was also significantly higher in treatment groups than in control rats from 8 weeks. The hepatocyte apoptosis was found to be significantly higher in the portal area of the liver tissue and around the central vein. However, spearman's rank correlation coefficient revealed that there was a mildly negative correlation between the iron index and hepatocyte apoptosis. CONCLUSIONS: This present study confirmed that hepatic iron overload was found during C. sinensis infection. This suggests that iron overload may be associated with hepatocyte apoptosis and involved in liver injury during C. sinensis infection. Further studies are needed to investigate the molecular mechanism involved here.
[Mh] Termos MeSH primário: Clonorquíase/patologia
Clonorchis sinensis/patogenicidade
Hepatócitos/patologia
Sobrecarga de Ferro/patologia
[Mh] Termos MeSH secundário: Animais
Apoptose
Clonorquíase/metabolismo
Hepatócitos/parasitologia
Seres Humanos
Ferro
Fígado/metabolismo
Fígado/patologia
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
E1UOL152H7 (Iron)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2630-3


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[PMID]:28542159
[Au] Autor:Kim EM; Kwak YS; Yi MH; Kim JY; Sohn WM; Yong TS
[Ad] Endereço:Department of Environmental Medical Biology and Arthropods of Medical Importance Resource Research Bank, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, Korea.
[Ti] Título:Clonorchis sinensis antigens alter hepatic macrophage polarization in vitro and in vivo.
[So] Source:PLoS Negl Trop Dis;11(5):e0005614, 2017 May.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Clonorchis sinensis infection elicits hepatic inflammation, which can lead to cholangitis, periductal hepatic fibrosis, liver cirrhosis, and even cholangiocarcinoma. Hepatic macrophages are an intrinsic element of both innate and acquired immunity. This study was conducted to demonstrate the dynamics of hepatic macrophage polarization during C. sinensis infection in mice and to identify factors regulating this polarization. Treatment of hepatic macrophages isolated from normal mice with C. sinensis excretory/secretory products (ESPs) resulted in the preferential generation of classically activated hepatic macrophages (M1 macrophages) and the production of pro-inflammatory cytokines. Additionally, cells stimulated with C. sinensis ESPs exhibited changes in cellular morphology. During the early stages of C. sinensis infection, hepatic macrophages preferentially differentiated into M1 macrophages; however, during the C. sinensis mature worm stage, when eggs are released, there were significant increases in the abundance of both M1 macrophages and alternatively activated hepatic macrophages (M2 macrophages). Moreover, there was a further increase in the M2 macrophage count during the fibrotic and cirrhotic stage of infection. Notably, this fibrotic and cirrhotic stage promoted a strong increase in the proportion of Arg-1-producing macrophages (M2 phenotype), which were associated with fibrosis and tissue repair in the liver. Our results suggest that the dynamic polarization of hepatic macrophages as C. sinensis infection progresses is related to the histological lesions present in liver tissue. Hepatic macrophages thus play an important role in local immunity during C. sinensis infection.
[Mh] Termos MeSH primário: Antígenos de Helmintos/imunologia
Clonorchis sinensis/imunologia
Fígado/patologia
Fígado/parasitologia
Macrófagos/imunologia
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Ativação de Macrófagos
Masculino
Camundongos Endogâmicos BALB C
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Helminth)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005614


  4 / 658 MEDLINE  
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[PMID]:28537229
[Au] Autor:Chelomina GN
[Ad] Endereço:Institute of Biology and Soil Science, Far East Branch, Russian Academy of Sciences, Vladivostok, 690022 Russia.
[Ti] Título:[Genomics and transcriptomics of the Chinese liver fluke Clonorchis sinensis (Opisthorchiidae, Trematoda)].
[So] Source:Mol Biol (Mosk);51(2):215-226, 2017 Mar-Apr.
[Is] ISSN:0026-8984
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The review summarizes the results of first genomic and transcriptomic investigations of the liver fluke Clonorchis sinensis (Opisthorchiidae, Trematoda). The studies mark the dawn of the genomic era for opisthorchiids, which cause severe hepatobiliary diseases in humans and animals. Their results aided in understanding the molecular mechanisms of adaptation to parasitism, parasite survival in mammalian biliary tracts, and genome dynamics in the individual development and the development of parasite-host relationships. Special attention is paid to the achievements in studying the codon usage bias and the roles of mobile genetic elements (MGEs) and small interfering RNAs (siRNAs). Interspecific comparisons at the genomic and transcriptomic levels revealed molecular differences, which may contribute to understanding the specialized niches and physiological needs of the respective species. The studies in C. sinensis provide a basis for further basic and applied research in liver flukes and, in particular, the development of efficient means to prevent, diagnose, and treat clonorchiasis.
[Mh] Termos MeSH primário: Adaptação Biológica/fisiologia
Clonorchis sinensis/genética
Genoma Helmíntico/fisiologia
Transcriptoma/fisiologia
[Mh] Termos MeSH secundário: Animais
Clonorquíase/genética
Clonorquíase/metabolismo
Clonorquíase/terapia
Clonorchis sinensis/metabolismo
Perfilação da Expressão Gênica
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE
[do] DOI:10.7868/S0026898417020070


  5 / 658 MEDLINE  
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[PMID]:28506040
[Au] Autor:Bae YA
[Ad] Endereço:Department of Microbiology, Gachon University College of Medicine, Incheon 21936, Korea.
[Ti] Título:Codon Usage Patterns of Tyrosinase Genes in .
[So] Source:Korean J Parasitol;55(2):175-183, 2017 Apr.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Codon usage bias (CUB) is a unique property of genomes and has contributed to the better understanding of the molecular features and the evolution processes of particular gene. In this study, genetic indices associated with CUB, including relative synonymous codon usage and effective numbers of codons, as well as the nucleotide composition, were investigated in the tyrosinase genes and their platyhelminth orthologs, which play an important role in the eggshell formation. The relative synonymous codon usage patterns substantially differed among tyrosinase genes examined. In a neutrality analysis, the correlation between GC and GC was statistically significant, and the regression line had a relatively gradual slope (0.218). NC-plot, i.e., GC vs effective number of codons (ENC), showed that most of the tyrosinase genes were below the expected curve. The codon adaptation index (CAI) values of the platyhelminth tyrosinases had a narrow distribution between 0.685/0.714 and 0.797/0.837, and were negatively correlated with their ENC. Taken together, these results suggested that CUB in the tyrosinase genes seemed to be basically governed by selection pressures rather than mutational bias, although the latter factor provided an additional force in shaping CUB of the and genes. It was also apparent that the equilibrium point between selection pressure and mutational bias is much more inclined to selection pressure in highly expressed genes, than in poorly expressed genes.
[Mh] Termos MeSH primário: Clonorchis sinensis/enzimologia
Clonorchis sinensis/genética
Códon/genética
Genoma Helmíntico/genética
Monofenol Mono-Oxigenase/genética
[Mh] Termos MeSH secundário: Animais
Composição de Bases
Códon/química
Evolução Molecular
Monofenol Mono-Oxigenase/fisiologia
Seleção Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon); EC 1.14.18.1 (Monophenol Monooxygenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.2.175


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[PMID]:28353615
[Au] Autor:Jeong JY; Lee JY; Chung BS; Choi Y; Alley AB; Kim HJ
[Ad] Endereço:aDepartment of Preventive Medicine, Okcheon-gun Public Health Center, Okcheon-gun, Chungcheongbuk-do bPublic Health Medical Service, Boramae Medical Center, Seoul National University College of Medicine, Seoul cInstitute of Health Policy and Management, Medical Research Center, Seoul National University, Seoul dDivision of Malaria and Parasitic Diseases, National Institute of Health, Centers for Disease Control and Prevention, Cheongju, Chungcheongbuk-do eTaean-gun Hospitalized Public Health Center, Taeangun, Chungcheongnam-do fSeoul National University College of Medicine, Seoul gDepartment of Nursing Science, Shinsung University, Dangjin-si, Chungcheongnam-do, South Korea.
[Ti] Título:A new method for estimating the prevalence of clonorchiasis in Korea: A proposal to replace arbitrary riverside sampling.
[So] Source:Medicine (Baltimore);96(13):e6536, 2017 Mar.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:South Korea presently uses an arbitrary sampling method to monitor the prevalence of Clonorchis sinensis infection in the endemic areas of the country. However, the present method is not standardized and focuses primarily on individuals who reside nearest to the mainstream river. We propose a new sampling method that combines cluster sampling with proportionate quota sampling to ensure that the entire endemic area is accurately represented. We tested the new method in Okcheon-gun, South Korea, and determined that the C sinensis infection prevalence (8.9%) in 2013 was higher than that (6.9%) estimated in 2012 when the arbitrary method was used. Additionally, no difference was observed in the prevalence based on the distance from the riverside areas, including branches and creeks, between the areas <1 and >1 km away from the riversides. Therefore, health authorities should place equal emphasis on all regions within the endemic areas. Based on the findings, we recommend the following: the clonorchiasis prevalence rate must be measured using probability sampling, (clear guidelines on survey coverage should be provided to include the riverside areas and all areas nearby branch streams, and regional cohorts should be created for continuous monitoring of prevalence rates across the region.
[Mh] Termos MeSH primário: Clonorquíase/epidemiologia
Clonorchis sinensis
Monitoramento Epidemiológico
[Mh] Termos MeSH secundário: Adulto
Idoso
Animais
Feminino
Seres Humanos
Masculino
Meia-Idade
Prevalência
República da Coreia/epidemiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170413
[Lr] Data última revisão:
170413
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000006536


  7 / 658 MEDLINE  
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[PMID]:28323213
[Au] Autor:Tang Z; Sun H; Chen T; Lin Z; Jiang H; Zhou X; Shi C; Pan H; Chang O; Ren P; Yu J; Li X; Xu J; Huang Y; Yu X
[Ad] Endereço:Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China; Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, Ministry of Education, Guangzhou, Guangdong, China; Provincial Engineering Technology Research Center for Biological Vector Contro
[Ti] Título:Oral delivery of Bacillus subtilis spores expressing cysteine protease of Clonorchis sinensis to grass carp (Ctenopharyngodon idellus): Induces immune responses and has no damage on liver and intestine function.
[So] Source:Fish Shellfish Immunol;64:287-296, 2017 May.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Clonorchis sinensis (C. sinensis) is a fish-borne trematode. Human can be infected by ingestion of C. sinensis metacercariae parasitized in grass carp (Ctenopharyngodon idella). For induction of effective oral immune responses, spores of Bacillus subtilis (B. subtilis) WB600 were utilized as vehicle to delivery CsCP (cysteine protease of C. sinensis) cooperated with CotC (B.s-CotC-CP), one of coat proteins, to the gastrointestinal tract. After routine culture of 8-12 h in LB medium, B. subtilis containing CotC-CsCP was transferred into the sporulation culture medium. SDS-PAGE, western blotting and the growth curve indicated that the best sporulation time of recombinant WB600 was 24-30 h at 37 °C with continuous shaking (250 rpm). Grass carp were fed with three levels of B.s-CotC-CP (1 × 10 , 1 × 10 , and 1 × 10 CFU g ) incorporated in the basal pellets diet. The commercial pellets or supplemented with spores just expressing CotC (1 × 10 CFU g ) were served as control diet. Our results showed that grass carp orally immunized with the feed-based B.s-CotC-CP developed a strong specific immune response with significantly (P < 0.05) higher levels of IgM in samples of serum, bile, mucus of surface and intestinal compared to the control groups. Abundant colonization spores expressing CsCP were found in hindgut that is conducive to absorption and presentation of antigen. Moreover, B. subtilis spores appeared to show no sign of toxicity or damage in grass carp. Our cercariae challenge experiments suggested that oral administration of spores expressing CsCP could develop an effective protection against C. sinensis in fish body. Therefore, this study demonstrated that the feed-based recombinant spores could trigger high levels of mucosal and humoral immunity, and would be a promising candidate vaccine against C. sinensis metacercariae formation in freshwater fish.
[Mh] Termos MeSH primário: Bacillus subtilis/genética
Carpas
Clonorquíase/veterinária
Cisteína Proteases/metabolismo
Suplementos Nutricionais
Doenças dos Peixes/prevenção & controle
Esporos Bacterianos/imunologia
[Mh] Termos MeSH secundário: Administração Oral
Animais
Bacillus subtilis/metabolismo
Clonorquíase/imunologia
Clonorquíase/parasitologia
Clonorquíase/prevenção & controle
Clonorchis sinensis/química
Dieta/veterinária
Doenças dos Peixes/imunologia
Doenças dos Peixes/parasitologia
Proteínas de Helminto/metabolismo
Imunidade Humoral
Imunidade nas Mucosas
Organismos Geneticamente Modificados
Probióticos
Distribuição Aleatória
Esporos Bacterianos/genética
Vacinas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Helminth Proteins); 0 (Vaccines); EC 3.4.- (Cysteine Proteases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE


  8 / 658 MEDLINE  
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[PMID]:28273846
[Au] Autor:Kim YJ; Yoo WG; Lee MR; Kang JM; Na BK; Cho SH; Park MY; Ju JW
[Ad] Endereço:Division of Malaria and Parasitic Diseases, Centre for Immunology and Pathology, Korea National Research Institute of Health, Chungbuk 28159, Korea. hoiyui25@gmail.com.
[Ti] Título:Molecular and Structural Characterization of the Tegumental 20.6-kDa Protein in Clonorchis sinensis as a Potential Druggable Target.
[So] Source:Int J Mol Sci;18(3), 2017 Mar 04.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The tegument, representing the membrane-bound outer surface of platyhelminth parasites, plays an important role for the regulation of the host immune response and parasite survival. A comprehensive understanding of tegumental proteins can provide drug candidates for use against helminth-associated diseases, such as clonorchiasis caused by the liver fluke . However, little is known regarding the physicochemical properties of teguments. In this study, a novel 20.6-kDa tegumental protein of the adult worm (CsTegu20.6) was identified and characterized by molecular and in silico methods. The complete coding sequence of 525 bp was derived from cDNA clones and encodes a protein of 175 amino acids. Homology search using BLASTX showed CsTegu20.6 identity ranging from 29% to 39% with previously-known tegumental proteins in . Domain analysis indicated the presence of a calcium-binding EF-hand domain containing a basic helix-loop-helix structure and a dynein light chain domain exhibiting a ferredoxin fold. We used a modified method to obtain the accurate tertiary structure of the CsTegu20.6 protein because of the unavailability of appropriate templates. The CsTegu20.6 protein sequence was split into two domains based on the disordered region, and then, the structure of each domain was modeled using I-TASSER. A final full-length structure was obtained by combining two structures and refining the whole structure. A refined CsTegu20.6 structure was used to identify a potential CsTegu20.6 inhibitor based on protein structure-compound interaction analysis. The recombinant proteins were expressed in and purified by nickel-nitrilotriacetic acid affinity chromatography. In , CsTegu20.6 mRNAs were abundant in adult and metacercariae, but not in the egg. Immunohistochemistry revealed that CsTegu20.6 localized to the surface of the tegument in the adult fluke. Collectively, our results contribute to a better understanding of the structural and functional characteristics of CsTegu20.6 and homologs of flukes. One compound is proposed as a putative inhibitor of CsTegu20.6 to facilitate further studies for anthelmintics.
[Mh] Termos MeSH primário: Anti-Helmínticos/química
Clonorchis sinensis
Proteínas de Helminto/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anti-Helmínticos/farmacologia
Sítios de Ligação
Cálcio
Clonorquíase/parasitologia
Clonorchis sinensis/genética
Clonorchis sinensis/metabolismo
Simulação por Computador
Descoberta de Drogas
Proteínas de Helminto/antagonistas & inibidores
Proteínas de Helminto/genética
Proteínas de Helminto/metabolismo
Modelos Moleculares
Conformação Molecular
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Multimerização Proteica
Transporte Proteico
Reprodutibilidade dos Testes
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Helminth Proteins); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE


  9 / 658 MEDLINE  
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[PMID]:28253272
[Au] Autor:Lai YS; Zhou XN; Pan ZH; Utzinger J; Vounatsou P
[Ad] Endereço:Swiss Tropical and Public Health Institute, Basel, Switzerland.
[Ti] Título:Risk mapping of clonorchiasis in the People's Republic of China: A systematic review and Bayesian geostatistical analysis.
[So] Source:PLoS Negl Trop Dis;11(3):e0005239, 2017 Mar.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Clonorchiasis, one of the most important food-borne trematodiases, affects more than 12 million people in the People's Republic of China (P.R. China). Spatially explicit risk estimates of Clonorchis sinensis infection are needed in order to target control interventions. METHODOLOGY: Georeferenced survey data pertaining to infection prevalence of C. sinensis in P.R. China from 2000 onwards were obtained via a systematic review in PubMed, ISI Web of Science, Chinese National Knowledge Internet, and Wanfang Data from January 1, 2000 until January 10, 2016, with no restriction of language or study design. Additional disease data were provided by the National Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention in Shanghai. Environmental and socioeconomic proxies were extracted from remote-sensing and other data sources. Bayesian variable selection was carried out to identify the most important predictors of C. sinensis risk. Geostatistical models were applied to quantify the association between infection risk and the predictors of the disease, and to predict the risk of infection across P.R. China at high spatial resolution (over a grid with grid cell size of 5×5 km). PRINCIPAL FINDINGS: We obtained clonorchiasis survey data at 633 unique locations in P.R. China. We observed that the risk of C. sinensis infection increased over time, particularly from 2005 onwards. We estimate that around 14.8 million (95% Bayesian credible interval 13.8-15.8 million) people in P.R. China were infected with C. sinensis in 2010. Highly endemic areas (≥ 20%) were concentrated in southern and northeastern parts of the country. The provinces with the highest risk of infection and the largest number of infected people were Guangdong, Guangxi, and Heilongjiang. CONCLUSIONS/SIGNIFICANCE: Our results provide spatially relevant information for guiding clonorchiasis control interventions in P.R. China. The trend toward higher risk of C. sinensis infection in the recent past urges the Chinese government to pay more attention to the public health importance of clonorchiasis and to target interventions to high-risk areas.
[Mh] Termos MeSH primário: Clonorquíase/epidemiologia
Clonorchis sinensis/isolamento & purificação
Topografia Médica
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
China/epidemiologia
Demografia
Seres Humanos
Prevalência
Medição de Risco
Análise Espacial
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005239


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[PMID]:28249599
[Au] Autor:Chung EJ; Jeong YI; Lee MR; Kim YJ; Lee SE; Cho SH; Lee WJ; Park MY; Ju JW
[Ad] Endereço:Division of Malaria and Parasitic Diseases, Center for Immunology and Pathology, National Research Institute of Health, Korea Centers for Disease Control & Prevention, Osong, 28159, Republic of Korea.
[Ti] Título:Heat shock proteins 70 and 90 from Clonorchis sinensis induce Th1 response and stimulate antibody production.
[So] Source:Parasit Vectors;10(1):118, 2017 Mar 01.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Heat shock proteins (HSPs) are found in all prokaryotes and most compartments of eukaryotic cells. Members of the HSP family mediate immune responses to tissue damage or cellular stress. However, little is known about the immune response induced by the oriental liver fluke, Clonorchis sinensis, even though this organism is carcinogenic to humans. We address this issue in the present study in mouse bone marrow dendritic cells (mBMDCs), using recombinant HSP70 and 90 from C. sinensis (rCsHSP70 and rCsHSP90). METHODS: rCsHSP70 and rCsHSP90 were produced in an E. coli system. Purified recombinant proteins were treated in BMDCs isolated from C57BL/6 mice. T cells were isolated from Balb/c mice and co-cultured with activated mBMDCs. Expression of surface molecules was measured by flow cytometry and cytokine secretion was quantified using ELISA. C57BL/6 mice were divided into four groups, including peptide alone, peptide/Freund's adjuvant, peptide/CsHSP70, peptide/CsHSP90, and were immunized intraperitoneally three times. Two weeks after final immunization, antibodies against peptide were measured using ELISA. RESULTS: Both proteins induced a dose-dependent upregulation in major histocompatibility complex and co-stimulatory molecule expression and increased secretion of pro-inflammatory cytokines including interleukin (IL)-1ß, -6, and -12p70 and tumor necrosis factor-α in mBMDCs. Furthermore, when allogenic T cells were incubated with mBMDCs activated by rCsHSP70 and rCsHSP90, the helper T cell (Th)1 cytokine interferon-γ was up-regulated whereas the level of the Th2 cytokine IL-4 was unchanged. These results indicate that rCsHSPs predominantly induce a Th1 response. Over and above these results, we also demonstrated that the production of peptide-specific antibodies can be activated after immunization via in vitro peptide binding with rCsHSP70 or rCsHSP90. CONCLUSION: This study showed for the first time that the HSP or HSP/peptide complexes of C. sinensis could be considered as a more effective vaccine against C. sinensis infection as results of the activator of host immune response as well as the adjuvant for antigenic peptide conjugate to induce peptide-specific antibody response in mice.
[Mh] Termos MeSH primário: Anticorpos Anti-Helmínticos/imunologia
Clonorquíase/imunologia
Clonorchis sinensis/imunologia
Proteínas de Choque Térmico HSP70/imunologia
Proteínas de Choque Térmico HSP90/imunologia
[Mh] Termos MeSH secundário: Animais
Clonorquíase/parasitologia
Clonorchis sinensis/genética
Células Dendríticas/imunologia
Proteínas de Choque Térmico HSP70/administração & dosagem
Proteínas de Choque Térmico HSP70/genética
Proteínas de Choque Térmico HSP90/administração & dosagem
Proteínas de Choque Térmico HSP90/genética
Seres Humanos
Imunização
Interferon gama/genética
Interferon gama/imunologia
Interleucina-4/genética
Interleucina-4/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Células Th1/imunologia
Células Th2/imunologia
Vacinas/administração & dosagem
Vacinas/genética
Vacinas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (HSP70 Heat-Shock Proteins); 0 (HSP90 Heat-Shock Proteins); 0 (Recombinant Proteins); 0 (Vaccines); 207137-56-2 (Interleukin-4); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2026-7



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