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Pesquisa : B01.050.500.500.736.847.610 [Categoria DeCS]
Referências encontradas : 1027 [refinar]
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  1 / 1027 MEDLINE  
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[PMID]:29257948
[Au] Autor:Cutie S; Hoang AT; Payumo AY; Huang GN
[Ad] Endereço:Cardiovascular Research Institute and Department of Physiology, University of California San Francisco, San Francisco, CA 94158, USA; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94158, USA.
[Ti] Título:Unconventional Functions of Muscles in Planarian Regeneration.
[So] Source:Dev Cell;43(6):657-658, 2017 Dec 18.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Muscles are traditionally considered in the context of force generation. Scimone et al. (2017), reporting in Nature, now examine muscles in a developmental setting and find unexpected roles for distinct planarian muscle fibers. The authors show that muscles provide patterning signals to promote regeneration and guide tissue growth after injury.
[Mh] Termos MeSH primário: Planárias/fisiologia
Regeneração/fisiologia
[Mh] Termos MeSH secundário: Animais
Padronização Corporal/fisiologia
Diferenciação Celular/fisiologia
Fibras Musculares Esqueléticas/fisiologia
Músculos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


  2 / 1027 MEDLINE  
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[PMID]:28455125
[Au] Autor:Amaning-Kwarteng AO; Asif-Malik A; Pei Y; Canales JJ
[Ad] Endereço:Department of Neuroscience, Psychology and Behaviour, University of Leicester, Lancaster Road, Leicester LE1 9HN, United Kingdom.
[Ti] Título:Relapse to cocaine seeking in an invertebrate.
[So] Source:Pharmacol Biochem Behav;157:41-46, 2017 Jun.
[Is] ISSN:1873-5177
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Addiction is characterised by cycles of compulsive drug taking, periods of abstinence and episodes of relapse. The extinction/reinstatement paradigm has been extensively used in rodents to model human relapse and explore underlying mechanisms and therapeutics. However, relapse to drug seeking behaviour has not been previously demonstrated in invertebrates. Here, we used a cocaine conditioned place preference (CPP) paradigm in the flatworm, planarian, followed by extinction and reinstatement of drug seeking. Once baseline preference was established for one of two distinctly textured environments (i.e. compartments with a coarse or smooth surface), planarian received pairings of cocaine (5µM) in the non-preferred, and vehicle in the most preferred, environment, and were tested for conditioning thereafter. Cocaine produced robust CPP, measured as a significant increase in the time spent in the cocaine-paired compartment. Subsequently, planarian underwent extinction training, reverting back to their original preference within three sessions. Brief exposure to cocaine (5µM) or methamphetamine (5µM) reinstated cocaine-seeking behaviour. By contrast, the high affinity dopamine transporter inhibitor, (N-(n-butyl)-3α-[bis (4-fluorophenyl) methoxy]-tropane) (JHW007), which in rodents exhibits a neurochemical and behavioural profile distinct from cocaine, was ineffective. The present findings demonstrate for the first time reinstatement of extinguished cocaine seeking in an invertebrate model and suggest that the long-term adaptations underlying drug conditioning and relapse are highly conserved through evolution.
[Mh] Termos MeSH primário: Cocaína/farmacologia
Condicionamento Operante/efeitos dos fármacos
Comportamento de Procura de Droga/efeitos dos fármacos
Extinção Psicológica/efeitos dos fármacos
Locomoção/efeitos dos fármacos
Reforço (Psicologia)
[Mh] Termos MeSH secundário: Animais
Comportamento Aditivo/psicologia
Condicionamento Operante/fisiologia
Comportamento de Procura de Droga/fisiologia
Extinção Psicológica/fisiologia
Invertebrados
Locomoção/fisiologia
Planárias
Recidiva
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
I5Y540LHVR (Cocaine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  3 / 1027 MEDLINE  
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[PMID]:28467318
[Au] Autor:Yang X; Kaj KJ; Schwab DJ; Collins ES
[Ad] Endereço:Department of Physics and Astronomy, Northwestern University, Evanston, IL, United States of America. These authors contributed equally to this work.
[Ti] Título:Coordination of size-control, reproduction and generational memory in freshwater planarians.
[So] Source:Phys Biol;14(3):036003, 2017 May 23.
[Is] ISSN:1478-3975
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Uncovering the mechanisms that control size, growth, and division rates of organisms reproducing through binary division means understanding basic principles of their life cycle. Recent work has focused on how division rates are regulated in bacteria and yeast, but this question has not yet been addressed in more complex, multicellular organisms. We have, over the course of several years, assembled a unique large-scale data set on the growth and asexual reproduction of two freshwater planarian species, Dugesia japonica and Girardia tigrina, which reproduce by transverse fission and succeeding regeneration of head and tail pieces into new planarians. We show that generation-dependent memory effects in planarian reproduction need to be taken into account to accurately capture the experimental data. To achieve this, we developed a new additive model that mixes multiple size control strategies based on planarian size, growth, and time between divisions. Our model quantifies the proportions of each strategy in the mixed dynamics, revealing the ability of the two planarian species to utilize different strategies in a coordinated manner for size control. Additionally, we found that head and tail offspring of both species employ different mechanisms to monitor and trigger their reproduction cycles. Thus, we find a diversity of strategies not only between species but between heads and tails within species. Our additive model provides two advantages over existing 2D models that fit a multivariable splitting rate function to the data for size control: firstly, it can be fit to relatively small data sets and can thus be applied to systems where available data is limited. Secondly, it enables new biological insights because it explicitly shows the contributions of different size control strategies for each offspring type.
[Mh] Termos MeSH primário: Tamanho Corporal
Planárias/fisiologia
Regeneração
Reprodução Assexuada
[Mh] Termos MeSH secundário: Animais
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1088/1478-3975/aa70c4


  4 / 1027 MEDLINE  
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[PMID]:29184198
[Au] Autor:Arenas OM; Zaharieva EE; Para A; Vásquez-Doorman C; Petersen CP; Gallio M
[Ad] Endereço:Department of Neurobiology, Northwestern University, Evanston, IL, USA.
[Ti] Título:Activation of planarian TRPA1 by reactive oxygen species reveals a conserved mechanism for animal nociception.
[So] Source:Nat Neurosci;20(12):1686-1693, 2017 Dec.
[Is] ISSN:1546-1726
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:All animals must detect noxious stimuli to initiate protective behavior, but the evolutionary origin of nociceptive systems is not well understood. Here we show that noxious heat and irritant chemicals elicit robust escape behaviors in the planarian Schmidtea mediterranea and that the conserved ion channel TRPA1 is required for these responses. TRPA1-mutant Drosophila flies are also defective in noxious-heat responses. We find that either planarian or human TRPA1 can restore noxious-heat avoidance to TRPA1-mutant Drosophila, although neither is directly activated by heat. Instead, our data suggest that TRPA1 activation is mediated by H O and reactive oxygen species, early markers of tissue damage rapidly produced as a result of heat exposure. Together, our data reveal a core function for TRPA1 in noxious heat transduction, demonstrate its conservation from planarians to humans, and imply that animal nociceptive systems may share a common ancestry, tracing back to a progenitor that lived more than 500 million years ago.
[Mh] Termos MeSH primário: Nociceptividade/fisiologia
Planárias/fisiologia
Espécies Reativas de Oxigênio/farmacologia
Canal de Cátion TRPA1/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Aprendizagem da Esquiva/efeitos dos fármacos
Comportamento Animal/efeitos dos fármacos
Drosophila
Proteínas de Drosophila/genética
Peróxido de Hidrogênio/farmacologia
Nociceptividade/efeitos dos fármacos
Técnicas de Patch-Clamp
Interferência de RNA
Canal de Cátion TRPA1/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (Reactive Oxygen Species); 0 (TRPA1 Cation Channel); 0 (TRPA1 protein, human); 0 (TrpA1 protein, Drosophila); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1038/s41593-017-0005-0


  5 / 1027 MEDLINE  
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[PMID]:27771293
[Au] Autor:Chan JD; Zhang D; Liu X; Zarowiecki M; Berriman M; Marchant JS
[Ad] Endereço:Department of Pharmacology, University of Minnesota Medical School, MN 55455, United States.
[Ti] Título:Utilizing the planarian voltage-gated ion channel transcriptome to resolve a role for a Ca channel in neuromuscular function and regeneration.
[So] Source:Biochim Biophys Acta;1864(6):1036-1045, 2017 06.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The robust regenerative capacity of planarian flatworms depends on the orchestration of signaling events from early wounding responses through the stem cell enacted differentiative outcomes that restore appropriate tissue types. Acute signaling events in excitable cells play an important role in determining regenerative polarity, rationalized by the discovery that sub-epidermal muscle cells express critical patterning genes known to control regenerative outcomes. These data imply a dual conductive (neuromuscular signaling) and instructive (anterior-posterior patterning) role for Ca signaling in planarian regeneration. Here, to facilitate study of acute signaling events in the excitable cell niche, we provide a de novo transcriptome assembly from the planarian Dugesia japonica allowing characterization of the diverse ionotropic portfolio of this model organism. We demonstrate the utility of this resource by proceeding to characterize the individual role of each of the planarian voltage-operated Ca channels during regeneration, and demonstrate that knockdown of a specific voltage operated Ca channel (Ca 1B) that impairs muscle function uniquely creates an environment permissive for anteriorization. Provision of the full transcriptomic dataset should facilitate further investigations of molecules within the planarian voltage-gated channel portfolio to explore the role of excitable cell physiology on regenerative outcomes. This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech.
[Mh] Termos MeSH primário: Canais de Cálcio/genética
Ativação do Canal Iônico
Músculos/fisiologia
Planárias/fisiologia
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Sinalização do Cálcio
Músculos/inervação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Calcium Channels)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171214
[Lr] Data última revisão:
171214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161108
[St] Status:MEDLINE


  6 / 1027 MEDLINE  
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[PMID]:28976975
[Au] Autor:Su H; Sureda-Gomez M; Rabaneda-Lombarte N; Gelabert M; Xie J; Wu W; Adell T
[Ad] Endereço:MOE Key Laboratory of Protein Science, School of Life Sciences, Tsinghua University, Beijing, China.
[Ti] Título:A C-terminally truncated form of ß-catenin acts as a novel regulator of Wnt/ß-catenin signaling in planarians.
[So] Source:PLoS Genet;13(10):e1007030, 2017 Oct.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ß-Catenin, the core element of the Wnt/ß-catenin pathway, is a multifunctional and evolutionarily conserved protein which performs essential roles in a variety of developmental and homeostatic processes. Despite its crucial roles, the mechanisms that control its context-specific functions in time and space remain largely unknown. The Wnt/ß-catenin pathway has been extensively studied in planarians, flatworms with the ability to regenerate and remodel the whole body, providing a 'whole animal' developmental framework to approach this question. Here we identify a C-terminally truncated ß-catenin (ß-catenin4), generated by gene duplication, that is required for planarian photoreceptor cell specification. Our results indicate that the role of ß-catenin4 is to modulate the activity of ß-catenin1, the planarian ß-catenin involved in Wnt signal transduction in the nucleus, mediated by the transcription factor TCF-2. This inhibitory form of ß-catenin, expressed in specific cell types, would provide a novel mechanism to modulate nuclear ß-catenin signaling levels. Genomic searches and in vitro analysis suggest that the existence of a C-terminally truncated form of ß-catenin could be an evolutionarily conserved mechanism to achieve a fine-tuned regulation of Wnt/ß-catenin signaling in specific cellular contexts.
[Mh] Termos MeSH primário: Planárias/fisiologia
Via de Sinalização Wnt
beta Catenina/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas do Domínio Armadillo/genética
Proteínas do Domínio Armadillo/metabolismo
Evolução Molecular
Homeostase
Modelos Biológicos
Fragmentos de Peptídeos/antagonistas & inibidores
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/metabolismo
Células Fotorreceptoras de Invertebrados/fisiologia
Planárias/genética
Planárias/crescimento & desenvolvimento
Domínios e Motivos de Interação entre Proteínas
Regeneração
Fatores de Transcrição TCF/genética
Fatores de Transcrição TCF/metabolismo
beta Catenina/antagonistas & inibidores
beta Catenina/genética
gama Catenina/genética
gama Catenina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Armadillo Domain Proteins); 0 (Peptide Fragments); 0 (TCF Transcription Factors); 0 (beta Catenin); 0 (gamma Catenin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007030


  7 / 1027 MEDLINE  
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[PMID]:28893948
[Au] Autor:Abnave P; Aboukhatwa E; Kosaka N; Thompson J; Hill MA; Aboobaker AA
[Ad] Endereço:Department of Zoology, Tinbergen Building, South Parks Road, University of Oxford, Oxford OX1 3PS, UK.
[Ti] Título:Epithelial-mesenchymal transition transcription factors control pluripotent adult stem cell migration in planarians.
[So] Source:Development;144(19):3440-3453, 2017 10 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Migration of stem cells underpins the physiology of metazoan animals. For tissues to be maintained, stem cells and their progeny must migrate and differentiate in the correct positions. This need is even more acute after tissue damage by wounding or pathogenic infection. Inappropriate migration also underpins metastasis. Despite this, few mechanistic studies address stem cell migration during repair or homeostasis in adult tissues. Here, we present a shielded X-ray irradiation assay that allows us to follow stem cell migration in planarians. We demonstrate the use of this system to study the molecular control of stem cell migration and show that , and EMT transcription factor homologs are necessary for cell migration to wound sites and for the establishment of migratory cell morphology. We also observed that stem cells undergo homeostatic migration to anterior regions that lack local stem cells, in the absence of injury, maintaining tissue homeostasis. This requires the polarity determinant Our work establishes planarians as a suitable model for further in-depth study of the processes controlling stem cell migration .
[Mh] Termos MeSH primário: Células-Tronco Adultas/citologia
Movimento Celular
Transição Epitelial-Mesenquimal
Planárias/citologia
Planárias/metabolismo
Células-Tronco Pluripotentes/citologia
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Células-Tronco Adultas/metabolismo
Células-Tronco Adultas/efeitos da radiação
Animais
Linhagem da Célula/efeitos da radiação
Movimento Celular/efeitos da radiação
Forma Celular/efeitos da radiação
Sequência Conservada
Epiderme/citologia
Transição Epitelial-Mesenquimal/efeitos da radiação
Cadeias beta de Integrinas/metabolismo
Metaloproteinases da Matriz/metabolismo
Planárias/genética
Células-Tronco Pluripotentes/efeitos da radiação
Fatores de Transcrição da Família Snail/metabolismo
Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Integrin beta Chains); 0 (Snail Family Transcription Factors); 0 (Transcription Factors); EC 3.4.24.- (Matrix Metalloproteinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1242/dev.154971


  8 / 1027 MEDLINE  
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[PMID]:28893535
[Au] Autor:Guo Q; Zhao G; Ni J; Guo Y; Zhang Y; Tian Q; Zhang S
[Ad] Endereço:School of LifeSciences, Zhengzhou University, Zhengzhou, Henan, China.
[Ti] Título:Down-regulate of Djrfc2 causes tissues hypertrophy during planarian regeneration.
[So] Source:Biochem Biophys Res Commun;493(3):1224-1229, 2017 Nov 25.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Planarians are an ideal model organism for regeneration research due to their amazing ability to regenerate. DNA replication is crucial for genome stability. Replication factor C (RFC), which is a replication factor C-like complex and plays an important role during DNA replication in eukaryotes, has been reported as a wound response factor during planarian regeneration. However, how RFC controls regeneration in planarians by regulating DNA replication remains to be explained. Here, we used a two-dimensional electrophoresis (2-DE) proteomic approach to identify differentially expressed proteins in intact and regenerated planarians. Approximately 132 protein spots showed differences between intact and regenerative tissues. We selected 21 significantly expressed protein spots and processed them using TOF MS analysis. Finally, we cloned three of these candidate genes (Djhsp70, Djrfc2, Djfaim), focusing on the function of Djrfc2 during regeneration. We found that the distribution of Djrfc2 tends toward the wound site. RNA interference (RNAi) of Djrfc2 increases the number of dividing cells and the expression level of planarian neoblast marker genes, which may result in hyper-proliferation. Our studies use an available approach to directly study the regeneration dynamic at the protein level and provide further evidence to support a function of Djrfc2 in planarian regeneration.
[Mh] Termos MeSH primário: Planárias/fisiologia
Regeneração/fisiologia
Proteína de Replicação C/genética
[Mh] Termos MeSH secundário: Animais
Proliferação Celular
Eletroforese em Gel Bidimensional
Regulação da Expressão Gênica
Planárias/genética
Proteômica
Interferência de RNA
Proteína de Replicação C/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.6.4.- (Replication Protein C)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE


  9 / 1027 MEDLINE  
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[PMID]:28844665
[Au] Autor:Dong Z; Yang T; Yang Y; Dou H; Chen G
[Ad] Endereço:College of Life Science, Henan Normal University, Xinxiang, 453007, Henan, China.
[Ti] Título:DjhnRNPA2/B1-like gene is required for planarian regeneration and tissue homeostasis.
[So] Source:Gene;633:9-16, 2017 Oct 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The hnRNPs play important roles in physiological processes in eukaryotic organisms by regulation of pre-mRNA after transcription, including pre-mRNA splicing, mRNA stability, DNA replication and repair and telomere maintenance and so on. However, it remains unclear about the specific functions of these genes. In this study, the full-length cDNA sequence of hnRNPA2/B1-like was first cloned from Dugesia japonica, and its roles were investigated by WISH and RNAi. The results showed that: (1) DjhnRNPA2/B1-like was highly conserved during animal evolution; (2) DjhnRNPA2/B1-like mRNA was mainly distributed each side of the body in intact worms and regenerative blastemas, and its expression levels were up-regulated on days 0 and 5 after amputation; (3) the intact and regenerating worms gradually lysed or lost regeneration capacity after DjhnRNPA2/B1-like RNAi; and (4) DjhnRNPA2/B1-like expression is induced by temperature and heavy metal ion stress. The data suggests that DjhnRNPA2/B1-like is a multiple functional gene, it plays important roles in regeneration and homeostatic maintenance and it is also involved in stress responses in planarians. Our work provides basic data for the study of regenerative mechanism and stress responses in freshwater planarians.
[Mh] Termos MeSH primário: Proteínas de Helminto/fisiologia
Ribonucleoproteínas Nucleares Heterogêneas/fisiologia
Homeostase/genética
Planárias/genética
Planárias/fisiologia
Regeneração/genética
[Mh] Termos MeSH secundário: Animais
DNA Complementar/genética
Proteínas de Helminto/classificação
Proteínas de Helminto/genética
Ribonucleoproteínas Nucleares Heterogêneas/classificação
Ribonucleoproteínas Nucleares Heterogêneas/genética
Hibridização In Situ
Metais Pesados/toxicidade
Interferência de RNA/fisiologia
Precursores de RNA/metabolismo
RNA Mensageiro/metabolismo
Homologia de Sequência de Aminoácidos
Estresse Fisiológico/genética
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Helminth Proteins); 0 (Heterogeneous-Nuclear Ribonucleoproteins); 0 (Metals, Heavy); 0 (RNA Precursors); 0 (RNA, Messenger)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE


  10 / 1027 MEDLINE  
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[PMID]:28807897
[Au] Autor:Bansal D; Kulkarni J; Nadahalli K; Lakshmanan V; Krishna S; Sasidharan V; Geo J; Dilipkumar S; Pasricha R; Gulyani A; Raghavan S; Palakodeti D
[Ad] Endereço:Institute for Stem Cell Biology and Regenerative Medicine, GKVK PO, Bellary Road, Bangalore 560065, India.
[Ti] Título:Cytoplasmic poly (A)-binding protein critically regulates epidermal maintenance and turnover in the planarian .
[So] Source:Development;144(17):3066-3079, 2017 09 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Identifying key cellular events that facilitate stem cell function and tissue organization is crucial for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of regeneration, such as epithelialization and epidermal organization are critically regulated by a novel cytoplasmic poly A-binding protein, SMED-PABPC2. Knockdown leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing, resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling suggests that epidermal lineage transcripts, including , are translationally regulated by SMED-PABPC2 Together, our results uncover a novel role for SMED-PABPC2 in the maintenance of epidermal and ECM integrity, critical for wound healing and subsequent processes for regeneration.
[Mh] Termos MeSH primário: Citoplasma/metabolismo
Epiderme/metabolismo
Planárias/metabolismo
Proteína I de Ligação a Poli(A)/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem da Célula
Proliferação Celular
Epitélio/metabolismo
Matriz Extracelular/metabolismo
Técnicas de Silenciamento de Genes
Homeostase
Modelos Biológicos
Planárias/genética
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Regeneração
Cicatrização
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Poly(A)-Binding Protein I); 0 (RNA, Messenger)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1242/dev.152942



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