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[PMID]:28987405
[Au] Autor:Botelho MJ; Vale C; Joaquim S; Costa ST; Soares F; Roque C; Matias D
[Ad] Endereço:IPMA, Portuguese Institute for the Sea and Atmosphere, Rua Alfredo Magalhães Ramalho, 6, 1495-006, Lisbon, Portugal; CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal. Electronic address: mjbotelho@ipma.pt.
[Ti] Título:Combined effect of temperature and nutritional regime on the elimination of the lipophilic toxin okadaic acid in the naturally contaminated wedge shell Donax trunculus.
[So] Source:Chemosphere;190:166-173, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The influence of nutritional regime and water temperature on depuration rates of OA-group toxins in the wedge shell Donax trunculus was examined by exposing naturally contaminated specimens to three nutritional regimes (microalgae, commercial paste of microalgae, and starvation) for 14 days at 16 °C and 20 °C. Total OA was quantified in the whole soft tissues of the individuals collected in days 2, 4, 6, 8, 10, 12 and 14. Mortality, dry weight, condition index, gross biochemical composition and gametogenic stages were surveyed. Low variation of glycogen and carbohydrates during the experiments suggest that wedge shells were under non-dramatic stress conditions. Wedge shells fed with non-toxic diets showed similar depuration rates being 15 and 38% higher than in starvation, at 16 and 20 °C, respectively. Depuration rates under non-toxic diets at 20 °C were 71% higher than at 16 °C. These results highlight the influence of water temperature on the depuration rate of total OA accumulated by D. trunculus, even when the increase is of only 4 °C, as commonly observed in week time scales in the southern Portuguese coastal waters. These results open the possibility of a faster release of OA in harvested wedge shells translocated to depuration systems when under a slight increase of water temperature.
[Mh] Termos MeSH primário: Bivalves/química
Recuperação e Remediação Ambiental/métodos
Avaliação Nutricional
Ácido Okadáico/isolamento & purificação
Temperatura Ambiente
[Mh] Termos MeSH secundário: Animais
Carboidratos/análise
Dieta/efeitos adversos
Glicogênio/análise
Toxinas Marinhas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Marine Toxins); 1W21G5Q4N2 (Okadaic Acid); 9005-79-2 (Glycogen)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


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[PMID]:29358154
[Au] Autor:Turner AD; Waack J; Lewis A; Edwards C; Lawton L
[Ad] Endereço:Centre for Environment, Fisheries and Aquaculture Science, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom. Electronic address: andrew.turner@cefas.co.uk.
[Ti] Título:Development and single-laboratory validation of a UHPLC-MS/MS method for quantitation of microcystins and nodularin in natural water, cyanobacteria, shellfish and algal supplement tablet powders.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1074-1075:111-123, 2018 Feb 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A simple, rapid UHPLC-MS/MS method has been developed and optimised for the quantitation of microcystins and nodularin in wide variety of sample matrices. Microcystin analogues targeted were MC-LR, MC-RR, MC-LA, MC-LY, MC-LF, LC-LW, MC-YR, MC-WR, [Asp3] MC-LR, [Dha7] MC-LR, MC-HilR and MC-HtyR. Optimisation studies were conducted to develop a simple, quick and efficient extraction protocol without the need for complex pre-analysis concentration procedures, together with a rapid sub 5min chromatographic separation of toxins in shellfish and algal supplement tablet powders, as well as water and cyanobacterial bloom samples. Validation studies were undertaken on each matrix-analyte combination to the full method performance characteristics following international guidelines. The method was found to be specific and linear over the full calibration range. Method sensitivity in terms of limits of detection, quantitation and reporting were found to be significantly improved in comparison to LC-UV methods and applicable to the analysis of each of the four matrices. Overall, acceptable recoveries were determined for each of the matrices studied, with associated precision and within-laboratory reproducibility well within expected guidance limits. Results from the formalised ruggedness analysis of all available cyanotoxins, showed that the method was robust for all parameters investigated. The results presented here show that the optimised LC-MS/MS method for cyanotoxins is fit for the purpose of detection and quantitation of a range of microcystins and nodularin in shellfish, algal supplement tablet powder, water and cyanobacteria. The method provides a valuable early warning tool for the rapid, routine extraction and analysis of natural waters, cyanobacterial blooms, algal powders, food supplements and shellfish tissues, enabling monitoring labs to supplement traditional microscopy techniques and report toxicity results within a short timeframe of sample receipt. The new method, now accredited to ISO17025 standard, is simple, quick, applicable to multiple matrices and is highly suitable for use as a routine, high-throughout, fast turnaround regulatory monitoring tool.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Cianobactérias/química
Suplementos Nutricionais/análise
Microcistinas/análise
Peptídeos Cíclicos/análise
Frutos do Mar/análise
[Mh] Termos MeSH secundário: Animais
Produtos Biológicos/análise
Produtos Biológicos/química
Bivalves
Contaminação de Alimentos
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
Comprimidos
Espectrometria de Massas em Tandem/métodos
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Microcystins); 0 (Peptides, Cyclic); 0 (Tablets); 0 (Water Pollutants, Chemical); 0979BIK2QU (nodularin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE


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[PMID]:29360839
[Au] Autor:Petkeviciute R; Stunzenas V; Staneviciute G
[Ad] Endereço:P. B. Sivickis Laboratory of Parasitology, Nature Research Centre, Vilnius, Lithuania.
[Ti] Título:Comments on species divergence in the genus Sphaerium (Bivalvia) and phylogenetic affinities of Sphaerium nucleus and S. corneum var. mamillanum based on karyotypes and sequences of 16S and ITS1 rDNA.
[So] Source:PLoS One;13(1):e0191427, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chromosome, 16S and ITS1 rDNA sequence analyses were used to obtain reliable diagnostic characters and to clarify phylogenetic relationships of sphaeriid bivalves of the genus Sphaerium. The species studied were found to be diploid, with modal number 2n = 28 in S. nucleus and 2n = 30 in S. corneum var. mamillanum. Small, biarmed, C- negative B chromosomes were found in all studied populations of both species. Karyological and molecular markers revealed no differences between S. corneum s. str. and S. corneum var. mamillanum. No intraspecific differences were found in the basic karyotype of S. nucleus. Molecular analyses, however, uncovered three genetically distinct ITS1 lineages: one comprised of samples from Lithuania, Slovakia, and Russia, another from Czech, and a third from Ukraine. Additionally to known 16S haplotype from Ukraine, three new 16S haplotypes of S. nucleus were detected: one in the samples from Lithuania and Russia, one in Slovakian and one in Czech population. In the ITS1 phylogenetic tree, all branches of S. nucleus clustered in one clade. In the 16S phylogenetic tree, however, the haplotype of Czech S. nucleus formed a separate branch, distant from three other haplotypes of S. nucleus. Molecular results indicate that in the context of the Evolutionary Species Concept the S. nucleus morphospecies may represent a complex of separate taxa, however referring on the Biological Species Concept the genetic lineages could represent the intraspecific variability.
[Mh] Termos MeSH primário: Bivalves/classificação
Bivalves/genética
[Mh] Termos MeSH secundário: Animais
DNA Espaçador Ribossômico/genética
Europa Oriental
Evolução Molecular
Especiação Genética
Genética Populacional
Cariotipagem
Filogenia
RNA Ribossômico 16S/genética
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191427


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[PMID]:29396269
[Au] Autor:Sezer N; Kocaoglan HO; Kiliç Ö; Lacoue-Labarthe T; Belivermis M
[Ad] Endereço:Department of Biology, Faculty of Science, Istanbul University, 34134 Vezneciler, Istanbul, Turkey.
[Ti] Título:Acidified seawater increases accumulation of cobalt but not cesium in manila clam Ruditapes philippinarum.
[So] Source:J Environ Radioact;184-185:114-121, 2018 Apr.
[Is] ISSN:1879-1700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The pH of seawater around the world is expected to continue its decline in the near future in response to ocean acidification that is driven by heightened atmospheric CO emissions. Concomitantly, economically-important molluscs that live in coastal waters including estuaries and embayments, may be exposed to a wide assortment of contaminants, including trace metals and radionuclides. Seawater acidification may alter both the chemical speciation of select elements as well as the physiology of organisms, and may thus pose at risk to many shellfish species, including the manila clam Ruditapes philippinarum. The bioconcentration efficiency of two common radionuclides associated with the nuclear fuel cycle, Cs and Co, were investigated by exposing live clams to dissolved Cs and Co at control (pH = 8.1) and two lowered pH (pH = 7.8 and 7.5) levels using controlled aquaria. The uptake and depuration kinetics of the two radionuclides in the whole-body clam were followed for 21 and 35 days, respectively. At steady-state equilibrium, the concentration factor (CF ) for Co increased as the pH decreased (i.e. 130 ± 5, 194 ± 6, and 258 ± 10 at pH levels 8.1, 7.8 and 7.5, respectively), whereas the Cs uptake was not influenced by a change in pH conditions. During depuration, the lowest depuration rate constant of Co by the manila clam was observed at the intermediate pH of 7.8. An increase in the accumulation of Co at the intermediate pH value was thought to be caused mainly by the aragonitic shell of the clam, as well as the low salinity and alkalinity of seawater used in the experiment. Considering that accumulation consists of uptake and depuration, among the three pH conditions moderately acidified seawater enhanced most the accumulation of Co. Accumulation of Cs was not strongly influenced by a reduced pH condition, as represented by an analogous uptake constant rate and CF in each treatment. Such results suggest that future seawater pH values that are projected to be lower in the next decades, may pose a risk for calcium-bearing organisms such as shellfish.
[Mh] Termos MeSH primário: Bivalves/metabolismo
Cobalto/análise
Água do Mar/química
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Animais
Césio/metabolismo
Cobalto/metabolismo
Concentração de Íons de Hidrogênio
Salinidade
Alimentos Marinhos
Poluentes Químicos da Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); 1KSV9V4Y4I (Cesium); 3G0H8C9362 (Cobalt)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180204
[St] Status:MEDLINE


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[PMID]:29038046
[Au] Autor:Sun S; Li Q; Kong L; Yu H
[Ad] Endereço:Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
[Ti] Título:Multiple reversals of strand asymmetry in molluscs mitochondrial genomes, and consequences for phylogenetic inferences.
[So] Source:Mol Phylogenet Evol;118:222-231, 2018 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Strand asymmetry in nucleotide composition is a remarkable feature of animal mitochondrial genomes. The strand-specific bias in the nucleotide composition of the mtDNA has been known to be highly problematic for phylogenetic analyses. Here, the strand asymmetry was compared across 140 mollusc species and analyzed for a mtDNA fragment including twelve protein-coding genes. The analyses show that almost all species in Gastropoda (except Heterobranchia) and all species in Bivalvia present reversals of strand bias. The skew values on individual genes for all codon positions (P ), third codon positions (P ), and fourfold redundant third codon positions (P ) indicated that CG skews are the best indicators of strand asymmetry. The differences in the patterns of strand asymmetry significantly influenced the amino acid composition of the encoded proteins. These biases are most striking for the amino acids Valine, Cysteine, Asparagine and Threonines, which appear to have evolved asymmetrical exchanges in response to shifts in nucleotide composition. Molluscs with strong variability of genome architectures (ARs) are usually characterized by a reversal of the usual strand bias. Phylogenetic analyses show that reversals of asymmetric mutational constraints have consequences on the phylogenetic inferences, as taxa characterized by reverse strand bias (Heterobranchia and Bivalvia) tend to group together due to long-branch attraction (LBA) artifacts. Neutral Transitions Excluded (NTE) model did not overcome the problem of heterogeneous biases present in molluscs mt genomes, suggested it may not be appropriate for molluscs mt genome data. Further refinement phylogenetic models may help us better understand internal relationships among these diverse organisms.
[Mh] Termos MeSH primário: Genoma Mitocondrial
Moluscos/classificação
[Mh] Termos MeSH secundário: Aminoácidos/química
Aminoácidos/metabolismo
Animais
Composição de Bases
Bivalves/classificação
Bivalves/genética
Códon
Gastrópodes/classificação
Gastrópodes/genética
Moluscos/genética
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Codon)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE


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[PMID]:29284014
[Au] Autor:Katharios P; Kalatzis PG; Kokkari C; Sarropoulou E; Middelboe M
[Ad] Endereço:Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Crete, Greece.
[Ti] Título:Isolation and characterization of a N4-like lytic bacteriophage infecting Vibrio splendidus, a pathogen of fish and bivalves.
[So] Source:PLoS One;12(12):e0190083, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A novel virulent bacteriophage, vB_VspP_pVa5, infecting a strain of Vibrio splendidus was isolated from a sea-cage aquaculture farm in Greece, and characterized using microbiological methods and genomic analysis. Bacteriophage vB_VspP_pVa5 is a N4-like podovirus with an icosahedral head measuring 85 nm in length and a short non-contractile tail. The phage had a narrow host range infecting only the bacterial host, a latent period of 30 min and a burst size of 24 virions per infected bacterium. Its genome size was 78,145 bp and genomic analysis identified 107 densely-packed genes, 40 of which could be annotated. In addition to the very large virion encapsulated DNA-dependent RNA polymerase which is the signature of the N4-like genus, an interesting feature of the novel phage is the presence of a self-splicing group I intron in the thymidylate synthase gene. A tRNAStop interrupted by a ~2.5kb open reading frame-containing area was also identified. The absence of genes related to lysogeny along with the high efficacy observed during in vitro cell lysis trials, indicate that the vB_VspP_pVa5 is a potential candidate component in a bacteriophage cocktail suitable for the biological control of V. splendidus in aquaculture.
[Mh] Termos MeSH primário: Bacteriófagos/isolamento & purificação
Bivalves/microbiologia
Peixes/microbiologia
Vibrio/virologia
[Mh] Termos MeSH secundário: Animais
Aquicultura
Microscopia Eletrônica de Transmissão
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190083


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[PMID]:27775698
[Au] Autor:König S; Gros O; Heiden SE; Hinzke T; Thürmer A; Poehlein A; Meyer S; Vatin M; Mbéguié-A-Mbéguié D; Tocny J; Ponnudurai R; Daniel R; Becher D; Schweder T; Markert S
[Ad] Endereço:Department of Pharmaceutical Biotechnology, E.M.A. University of Greifswald, Institute of Pharmacy, Greifswald, Germany.
[Ti] Título:Nitrogen fixation in a chemoautotrophic lucinid symbiosis.
[So] Source:Nat Microbiol;2:16193, 2016 Oct 24.
[Is] ISSN:2058-5276
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The shallow water bivalve Codakia orbicularis lives in symbiotic association with a sulfur-oxidizing bacterium in its gills. The endosymbiont fixes CO and thus generates organic carbon compounds, which support the host's growth. To investigate the uncultured symbiont's metabolism and symbiont-host interactions in detail we conducted a proteogenomic analysis of purified bacteria. Unexpectedly, our results reveal a hitherto completely unrecognized feature of the C. orbicularis symbiont's physiology: the symbiont's genome encodes all proteins necessary for biological nitrogen fixation (diazotrophy). Expression of the respective genes under standard ambient conditions was confirmed by proteomics. Nitrogenase activity in the symbiont was also verified by enzyme activity assays. Phylogenetic analysis of the bacterial nitrogenase reductase NifH revealed the symbiont's close relationship to free-living nitrogen-fixing Proteobacteria from the seagrass sediment. The C. orbicularis symbiont, here tentatively named 'Candidatus Thiodiazotropha endolucinida', may thus not only sustain the bivalve's carbon demands. C. orbicularis may also benefit from a steady supply of fixed nitrogen from its symbiont-a scenario that is unprecedented in comparable chemoautotrophic symbioses.
[Mh] Termos MeSH primário: Bivalves/microbiologia
Crescimento Quimioautotrófico
Gammaproteobacteria/fisiologia
Fixação de Nitrogênio
Simbiose
[Mh] Termos MeSH secundário: Animais
Gammaproteobacteria/química
Gammaproteobacteria/genética
Gammaproteobacteria/metabolismo
Brânquias/microbiologia
Redes e Vias Metabólicas/genética
Nitrogenase/metabolismo
Oxirredutases/genética
Filogenia
Proteoma/análise
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proteome); EC 1.- (Oxidoreductases); EC 1.18.6.1 (Nitrogenase); EC 1.18.6.1 (nitrogenase reductase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1038/nmicrobiol.2016.193


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[PMID]:27775707
[Au] Autor:Petersen JM; Kemper A; Gruber-Vodicka H; Cardini U; van der Geest M; Kleiner M; Bulgheresi S; Mußmann M; Herbold C; Seah BK; Antony CP; Liu D; Belitz A; Weber M
[Ad] Endereço:Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, Research Network Chemistry meets Microbiology, University of Vienna, Althanstrasse 14, Vienna 1090, Austria.
[Ti] Título:Chemosynthetic symbionts of marine invertebrate animals are capable of nitrogen fixation.
[So] Source:Nat Microbiol;2:16195, 2016 Oct 24.
[Is] ISSN:2058-5276
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chemosynthetic symbioses are partnerships between invertebrate animals and chemosynthetic bacteria. The latter are the primary producers, providing most of the organic carbon needed for the animal host's nutrition. We sequenced genomes of the chemosynthetic symbionts from the lucinid bivalve Loripes lucinalis and the stilbonematid nematode Laxus oneistus. The symbionts of both host species encoded nitrogen fixation genes. This is remarkable as no marine chemosynthetic symbiont was previously known to be capable of nitrogen fixation. We detected nitrogenase expression by the symbionts of lucinid clams at the transcriptomic and proteomic level. Mean stable nitrogen isotope values of Loripes lucinalis were within the range expected for fixed atmospheric nitrogen, further suggesting active nitrogen fixation by the symbionts. The ability to fix nitrogen may be widespread among chemosynthetic symbioses in oligotrophic habitats, where nitrogen availability often limits primary productivity.
[Mh] Termos MeSH primário: Organismos Aquáticos/microbiologia
Bactérias/enzimologia
Bivalves/microbiologia
Cromadoria/microbiologia
Fixação de Nitrogênio
Simbiose
[Mh] Termos MeSH secundário: Animais
Bactérias/genética
Perfilação da Expressão Gênica
Nitrogenase/genética
Proteoma/análise
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proteome); EC 1.18.6.1 (Nitrogenase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1038/nmicrobiol.2016.195


  9 / 7816 MEDLINE  
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[PMID]:29311448
[Au] Autor:Yamamoto A; Kudo S; Nakaya M; Hanaishi R; Masuda Y; Kimura J; Sakuraba A; Shibata M; Kudo S; Itsukaichi T; Sato H; Murakami A; Kogawa A
[Ad] Endereço:Aomori Prefectural Public Health and Environment Center.
[Ti] Título:[Validation Study of Analytical Method for Determination of Amnesic Shellfish Poison in Bivalves].
[So] Source:Shokuhin Eiseigaku Zasshi;58(6):281-287, 2017.
[Is] ISSN:1882-1006
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:Amnesic shellfish poison (ASP) is regarded as one of the shellfish poison groups in the EU, though it is not subject to regulation in Japan. We have developed an analytical method of ASP based on the report by Hatfield et al. and other methods. Validation studies were carried out with certified compositional reference materials (CRM). Performance parameters were estimated based on 17 analytical results. The estimate of trueness was 97.5%, and the estimate of intralaboratory reproducibility (RSD) was 1.5%. The HorRat(r) value was 0.16. These performance parameters meet the criteria in the Codex Procedural Manual. Furthermore, internal quality control was performed by using the CRM. The action limits were set based on the performance parameters of the method. Most of the results of the internal quality control were within the action limit range. The results confirmed that the quality of the analyses was well maintained. The purpose of the analytical method is to confirm that the level of ASP in scallop is less than 4.6 mg/kg. The applicability of the analytical method to scallops was confirmed by using spiked samples.
[Mh] Termos MeSH primário: Bivalves/química
Cromatografia Líquida de Alta Pressão/métodos
Ácido Caínico/análogos & derivados
Intoxicação por Frutos do Mar/etiologia
Intoxicação por Frutos do Mar/prevenção & controle
Frutos do Mar/análise
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão/instrumentação
Monitoramento Ambiental/métodos
Ácido Caínico/análise
Ácido Caínico/química
Ácido Caínico/toxicidade
Pectinidae/química
Controle de Qualidade
Reprodutibilidade dos Testes
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
M02525818H (domoic acid); SIV03811UC (Kainic Acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.3358/shokueishi.58.281


  10 / 7816 MEDLINE  
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[PMID]:29261749
[Au] Autor:Featherstone AM; Butler PG; Peharda M; Chauvaud L; Thébault J
[Ad] Endereço:Universite de Bretagne Occidentale, Institut Universitaire Européen de la Mer, Laboratoire des sciences de l'environnement marin (LEMAR UMR 6539 CNRS/UBO/IRD/Ifremer), Plouzané, France.
[Ti] Título:Influence of riverine input on the growth of Glycymeris glycymeris in the Bay of Brest, North-West France.
[So] Source:PLoS One;12(12):e0189782, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A crossdated, replicated, chronology of 114 years (1901-2014) was developed from internal growth increments in the shells of Glycymeris glycymeris samples collected monthly from the Bay of Brest, France. Bivalve sampling was undertaken between 2014 and 2015 using a dredge. In total 401 live specimens and 243 articulated paired valves from dead specimens were collected, of which 38 individuals were used to build the chronology. Chronology strength, assessed as the Expressed Population Signal, was above 0.7 throughout, falling below the generally accepted threshold of 0.85 before 1975 because of reduced sample depth. Significant positive correlations were identified between the shell growth and the annual averages of rainfall (1975-2008; r = 0.34) and inflow from the river Elorn (1989-2009; r = 0.60). A significant negative correlation was identified between shell growth and the annual average salinity (1998-2014; r = -0.62). Analysis of the monthly averages indicates that these correlations are associated with the winter months (November-February) preceding the G. glycymeris growth season suggesting that winter conditions predispose the benthic environment for later shell growth. Concentration of suspended particulate matter within the river in February is also positively correlated with shell growth, leading to the conclusion that food availability is also important to the growth of G. glycymeris in the Bay of Brest. With the addition of principle components analysis, we were able to determine that inflow from the River Elorn, nitrite levels and salinity were the fundamental drivers of G. glycymeris growth and that these environmental parameters were all linked.
[Mh] Termos MeSH primário: Baías
Bivalves/crescimento & desenvolvimento
Rios
[Mh] Termos MeSH secundário: Animais
França
Geografia
Análise de Componente Principal
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189782



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