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[PMID]: 29107028 [Au] Autor: Pelin M; Kilcoyne J; Nulty C; Crain S; Hess P; Tubaro A; Sosa S [Ad] Endereço: Department of Life Sciences, University of Trieste, Via A. Valerio 6, 34127 Trieste, Italy. [Ti] Título: Toxic equivalency factors (TEFs) after acute oral exposure of azaspiracid 1, -2 and -3 in mice. [So] Source: Toxicol Lett;282:136-146, 2018 Jan 05. [Is] ISSN: 1879-3169 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Azaspiracids (AZAs) are marine algal toxins that can be accumulated by edible shellfish to cause a foodborne gastrointestinal poisoning in humans. In the European Union, only AZA1, -2 and -3 are currently regulated and their concentration in shellfish is determined through their toxic equivalency factors (TEFs) derived from the intraperitoneal lethal potency in mice. Nevertheless, considering the potential human exposure by oral route, AZAs TEFs should be calculated by comparative oral toxicity data. Thus, the acute oral toxicity of AZA1, -2 and -3 was investigated in female CD-1 mice treated with different doses (AZA1: 135-1100µg/kg; AZA2 and AZA3: 300-1100µg/kg) and sacrificed after 24h or 14days. TEFs derived from the median lethal doses (LD ) were 1.0, 0.7 and 0.5, respectively for AZA1, -2 and -3. In fact, after 24h from gavage administration, LD were 443µg/kg (AZA1; 95% CL: 350-561µg/kg), 626µg/kg (AZA2; 95% CL: 430-911µg/kg) and 875µg/kg (AZA3; 95% CL: 757-1010µg/kg). Mice dead more than 5h after the treatment or those sacrificed after 24h (doses: ≥175µg AZA1/kg, ≥500µg AZA2/kg and ≥600µg AZA3/kg) showed enlarged pale liver, while increased serum markers of liver alteration were recorded even at the lowest doses. Blood chemistry revealed significantly increased serum levels of K ions (≥500mg/kg), whereas light microscopy showed tissue changes in the gastrointestinal tract, liver and spleen. No lethality, macroscopic, tissue or haematological changes were recorded two weeks post exposure, indicating reversible toxic effects. LC-MS/MS analysis of the main organs showed a dose-dependency in gastrointestinal absorption of these toxins: at 24h, the highest levels were detected in the stomach and, in descending order, in the intestinal content, liver, small intestine, kidneys, lungs, large intestine, heart as well as detectable traces in the brain. After 14days, AZA1 and AZA2 were still detectable in almost all the organs and intestinal content. [Mh] Termos MeSH primário: Furanos/toxicidade Toxinas Marinhas/toxicidade Piranos/toxicidade Compostos de Espiro/toxicidade [Mh] Termos MeSH secundário: Administração Oral Animais Relação Dose-Resposta a Droga Feminino Furanos/farmacocinética Dose Letal Mediana Toxinas Marinhas/farmacocinética Camundongos Endogâmicos Mytilus edulis/química Especificidade de Órgãos Piranos/farmacocinética Compostos de Espiro/farmacocinética Distribuição Tecidual Testes de Toxicidade Aguda [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Furans); 0 (Marine Toxins); 0 (Pyrans); 0 (Spiro Compounds); 0 (azaspiracid); 0 (azaspiracid-2); 0 (azaspiracid-3) [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 171201 [Lr] Data última revisão: 171201 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171107 [St] Status: MEDLINE

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[PMID]: 28899748 [Au] Autor: Strehse JS; Appel D; Geist C; Martin HJ; Maser E [Ad] Endereço: Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School Schleswig-Holstein, Brunswiker Str. 10, 24105 Kiel, Germany. [Ti] Título: Biomonitoring of 2,4,6-trinitrotoluene and degradation products in the marine environment with transplanted blue mussels (M. edulis). [So] Source: Toxicology;390:117-123, 2017 Sep 01. [Is] ISSN: 1879-3185 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: Since World War I considerable amounts of warfare material have been dumped at sea worldwide, but little is known about the fate of the explosive components in the marine environment. Sea dumped munitions are able to contaminate the surroundings because of the release of explosive chemicals due to corrosion and breaching or by detonation after blast-operations. This implies the risk of accumulation of toxic compounds in human and wildlife food chains. With the help of divers, we performed an active biomonitoring study with transplanted blue mussels (M. edulis) in a burdened area (Kolberger Heide, Germany) with explosive compounds near blast craters over an exposure time of 93days. With this biomonitoring system, we could show that blue mussels accumulate 2,4,6-trinitrotoluene (TNT) and its metabolites 2-amino-4,6-dinitrotoluene (2-ADNT) and 4-amino-2,6-dinitrotoluene (4-ADNT) in their tissues. In all mussels deployed at the ground, we found a body burden with 2-ADNT of 103.75±12.77ng/g wet weight and with 4-ADNT of 131.31±9.53ng/g wet weight. TNT itself has been found in six mussels with an average concentration of 31.04±3.26ng/g mussel wet weight. In the mussels positioned at one meter above the ground no TNT nor 2-ADNT could be detected, but 4-ADNT was found in all samples with an average concentration of 8.71±2.88ng/g mussel wet weight. To the best of our knowledge, this is the first study using blue mussels M. edulis as an active biomonitoring system for TNT and its metabolites 2-ADNT and 4-ADNT in a free field experiment in a burdened area. Moreover, with this system, we unequivocally proved that these toxic explosives accumulate in the marine biota resp. in the marine food chain, thereby posing a possible risk to the marine ecosphere and human health. [Mh] Termos MeSH primário: Monitoramento Ambiental/métodos Mytilus edulis/metabolismo Trinitrotolueno/metabolismo Poluentes Químicos da Água/metabolismo Poluição da Água [Mh] Termos MeSH secundário: Compostos de Anilina/metabolismo Animais Biomarcadores/metabolismo Biotransformação Carga Corporal (Radioterapia) Cadeia Alimentar Contaminação de Alimentos Alemanha Seres Humanos Reprodutibilidade dos Testes Medição de Risco [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Aniline Compounds); 0 (Biomarkers); 0 (Water Pollutants, Chemical); 118-96-7 (Trinitrotoluene); 189OOM840S (2-amino-4,6-dinitrotoluene); 19406-51-0 (4-amino-2,6-dinitrotoluene) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171023 [Lr] Data última revisão: 171023 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170914 [St] Status: MEDLINE

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[PMID]: 28837417 [Au] Autor: Sahlmann A; Wolf R; Holth TF; Titelman J; Hylland K [Ad] Endereço: a Department of Biosciences , University of Oslo , Oslo , Norway. [Ti] Título: Baseline and oxidative DNA damage in marine invertebrates. [So] Source: J Toxicol Environ Health A;80(16-18):807-819, 2017. [Is] ISSN: 1528-7394 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Anthropogenic pollutants produce oxidative stress in marine organisms, directly or following generation of reactive oxygen species (ROS), potentially resulting in increased accumulation of DNA strand breaks quantified. The aim of this study is to quantify baseline levels of DNA strand breaks in marine species from four phyla and to assess relative sensitivity to oxidative stress as well as ability to recover. DNA strand breaks were determined using a formamidopyrimidine DNA glycosylase (Fpg)-amended comet assay in circulating cells from blue mussel (Mytilus edulis), shore crab (Carcinus maenas), sea star (Asterias rubens), and vase tunicate (Ciona intestinalis). Lymphocytes from Atlantic cod (Gadus morhua) were used as a reference. In addition to immediate analysis, cells from all species were exposed ex vivo to two concentrations of hydrogen peroxide (H O ) at 25 or 250 µM prior to assay. Mean baseline DNA strand breaks were highest for cells from sea star (34%) followed by crab (25%), mussel (22%), tunicate (17%), and cod (14%). Circulating cells from invertebrates were markedly more sensitive to oxidative stress compared to cod lymphocytes. DNA strand breaks exceeded 80% for sea star, crab, and mussel cells following exposure to the lowest H O concentration. There was no recovery for cells from any species following 1 hr in buffer. This study provides an in-depth analysis of DNA integrity for ecologically important species representing 4 phyla. Data indicate that circulating cells from invertebrates are more sensitive to oxidative stress than cells from fish as evidenced by DNA strand breaks. Future studies need to address the extent to which DNA strand breaks may exert consequences for body maintenance costs in marine invertebrates. [Mh] Termos MeSH primário: Organismos Aquáticos/efeitos dos fármacos Dano ao DNA/efeitos dos fármacos Peróxido de Hidrogênio/toxicidade Estresse Oxidativo/efeitos dos fármacos Poluentes Químicos da Água/toxicidade [Mh] Termos MeSH secundário: Animais Organismos Aquáticos/genética Asterias/efeitos dos fármacos Asterias/genética Braquiúros/efeitos dos fármacos Braquiúros/genética Ciona intestinalis/efeitos dos fármacos Ciona intestinalis/genética Ensaio Cometa DNA-Formamidopirimidina Glicosilase/metabolismo Determinação de Ponto Final Peixes/genética Linfócitos/efeitos dos fármacos Linfócitos/metabolismo Mytilus edulis/efeitos dos fármacos Mytilus edulis/genética Especificidade da Espécie [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Water Pollutants, Chemical); BBX060AN9V (Hydrogen Peroxide); EC 3.2.2.23 (DNA-Formamidopyrimidine Glycosylase) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171031 [Lr] Data última revisão: 171031 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170825 [St] Status: MEDLINE [do] DOI: 10.1080/15287394.2017.1352179

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[PMID]: 28737154 [Au] Autor: Liddell C; Welsh JE; van der Meer J; Thieltges DW [Ad] Endereço: NIOZ Royal Netherlands Institute for Sea Research, Department of Coastal Systems, and Utrecht University, PO Box 59, 1790 AB den Burg, Texel, The Netherlands. [Ti] Título: Effect of dose and frequency of exposure to infectious stages on trematode infection intensity and success in mussels. [So] Source: Dis Aquat Organ;125(2):85-92, 2017 Jul 24. [Is] ISSN: 0177-5103 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Marine parasites such as trematodes often compromise the fitness of their hosts. Such effects are generally considered to be density-dependent, i.e. the greater the infection intensity in the host, the greater the detrimental impact on host fitness. However, the mechanisms determining infection in marine hosts are still poorly understood. Here, we investigated the effect of cercarial dose and exposure frequency (single vs. trickle infections) of a marine trematode parasite, Himasthla elongata (Trematoda: Echinostomatidae), on infection intensity and success in its second intermediate host, the blue mussel Mytilus edulis, an abundant and widely distributed bivalve in European coastal waters. In our laboratory experiment, we tested 4 levels of parasite doses and showed that mussels faced higher parasite infection intensity at higher doses of cercarial exposure and that they acquired more infections when repeatedly exposed to smaller doses compared to a single high dose. However, the infection success of cercariae did not differ among 4 dose levels but was only significantly different between trickle and single exposures. This indicates that cercariae were not subjected to a dose-dependent regulation of their infectivity, suggesting that infection intensity in mussels is largely driven by factors mediating the abundance of infective stages. With the combined investigation of the effect of cercarial dose and exposure frequency at realistic dose levels, our study contributes to our currently very limited understanding of the determinants of infection intensity in marine hosts and highlights the usefulness of experimental studies in advancing our knowledge in this field. [Mh] Termos MeSH primário: Mytilus edulis/parasitologia Trematódeos/fisiologia [Mh] Termos MeSH secundário: Animais Interações Hospedeiro-Parasita [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171013 [Lr] Data última revisão: 171013 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170725 [St] Status: MEDLINE [do] DOI: 10.3354/dao03133

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[PMID]: 28525787 [Au] Autor: Van Ael E; Blust R; Bervoets L [Ad] Endereço: Laboratory of Systemic Physiological and Ecotoxicological Research, Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium. [Ti] Título: Metals in the Scheldt estuary: From environmental concentrations to bioaccumulation. [So] Source: Environ Pollut;228:82-91, 2017 Sep. [Is] ISSN: 1873-6424 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: To investigate the relationship between metal concentrations in abiotic compartments and in aquatic species, sediment, suspended matter and several aquatic species (Polychaeta, Oligochaeta, four crustacean species, three mollusc species and eight fish species) were collected during three seasons at six locations along the Scheldt estuary (the Netherlands-Belgium) and analysed on their metal content (Ag, Cd, Co, Cr, Cu, Ni, Pb, Zn and the metalloid As). Sediment and biota tissue concentrations were significantly influenced by sampling location, but not by season. Measurements of Acid Volatile Sulphides (AVS) concentrations in relation to Simultaneously Extracted Metals (SEM) in the sediment suggested that not all metals in the sediment will be bound to sulphides and some metals might be bioavailable. For all metals but zinc, highest concentrations were measured in invertebrate species; Ag and Ni in periwinkle, Cr, Co and Pb in Oligochaete worms and As, Cd and Cu in crabs and shrimp. Highest concentrations of Zn were measured in the kidney of European smelt. In fish, for most of the metals, the concentrations were highest in liver or kidney and lowest in muscle. For Zn however, highest concentrations were measured in the kidney of European smelt. For less than half of the metals significant correlations between sediment metal concentrations and bioaccumulated concentrations were found (liver/hepatopancreas or whole organism). To calculate the possible human health risk by consumption, average and maximum metal concentrations in the muscle tissues were compared to the minimum risk levels (MRLs). Concentrations of As led to the highest risk potential for all consumable species. Cadmium and Cu posed only a risk when consuming the highest contaminated shrimp and shore crabs. Consuming blue mussel could result in a risk for the metals As, Cd and Cr. [Mh] Termos MeSH primário: Monitoramento Ambiental Estuários Metais/análise Poluentes Químicos da Água/análise [Mh] Termos MeSH secundário: Animais Bélgica Braquiúros Cádmio/análise Meio Ambiente Peixes/metabolismo Sedimentos Geológicos/análise Seres Humanos Metais Pesados/análise Mytilus edulis/metabolismo Países Baixos Poliquetos/metabolismo Frutos do Mar/análise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Metals); 0 (Metals, Heavy); 0 (Water Pollutants, Chemical); 00BH33GNGH (Cadmium) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170808 [Lr] Data última revisão: 170808 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170520 [St] Status: MEDLINE

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[PMID]: 28500903 [Au] Autor: Kim YD; Kim WJ; Shin YK; Lee DH; Kim YJ; Kim JK; Rhee JS [Ad] Endereço: Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science, Tongyeong 46083, South Korea. [Ti] Título: Microcystin-LR bioconcentration induces antioxidant responses in the digestive gland of two marine bivalves Crassostrea gigas and Mytilus edulis. [So] Source: Aquat Toxicol;188:119-129, 2017 Jul. [Is] ISSN: 1879-1514 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Microcystins (MCs) are a major group of potent cyanobacterial toxins found in freshwater and even brackish waterbodies. To understand the putative correlation between bioconcentration of MCs and antioxidant responses of the digestive gland of bivalves, Pacific oyster Crassostrea gigas and blue mussel Mytilus edulis were exposed to different concentrations (0.1, 1, 10 and 20µgL ) of MC-Leucine-Arginine (LR) for seven days. MC-LR bioconcentrated in the digestive glands of both bivalves during exposure period. The levels were slightly reduced when the bivalves were exposed to seawater during depuration (7days), while approximately 0.1µgL of MC-LR was observed in the 10 and 20µgL exposed bivalves at the end of depuration. Intracellular malondialdehyde (MDA) and glutathione (GSH) levels were significantly elevated in the 10 and 20µgL exposed bivalves at 7day, and the levels were maintained during depuration in both bivalves. Overall, significant higher levels of enzymatic activities of antioxidant defense systems such as glutathione S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GR) were observed in the 10 and 20µgL exposed bivalves. Interestingly, most of higher levels of Pacific oyster were detected at exposure period, while blue mussel showed higher levels at depuration phase, suggesting a species-specific sensitivity upon MC-LR. These patterns were correlated with the bioconcentration patterns of MC-LR as Pacific oyster was highly accumulated by MC-LR during exposure period, but blue mussel showed prolonged high levels of MC-LR for depuration phase. Our results will be useful to understand species-specific bioconcentration of MC-LR in bivalves and their effects on intracellular oxidative status via accumulation. [Mh] Termos MeSH primário: Crassostrea/efeitos dos fármacos Trato Gastrointestinal/efeitos dos fármacos Microcistinas Mytilus edulis/efeitos dos fármacos Poluentes Químicos da Água [Mh] Termos MeSH secundário: Animais Catalase/metabolismo Crassostrea/metabolismo Trato Gastrointestinal/metabolismo Glutationa/metabolismo Glutationa Peroxidase/metabolismo Glutationa Redutase/metabolismo Glutationa Transferase/metabolismo Malondialdeído/metabolismo Microcistinas/farmacocinética Microcistinas/toxicidade Mytilus edulis/metabolismo Superóxido Dismutase/metabolismo Poluentes Químicos da Água/farmacocinética Poluentes Químicos da Água/toxicidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Microcystins); 0 (Water Pollutants, Chemical); 4Y8F71G49Q (Malondialdehyde); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); EC 1.8.1.7 (Glutathione Reductase); EC 2.5.1.18 (Glutathione Transferase); EQ8332842Y (cyanoginosin LR); GAN16C9B8O (Glutathione) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171003 [Lr] Data última revisão: 171003 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170514 [St] Status: MEDLINE

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[PMID]: 28441609 [Au] Autor: Gagné PL; Fortier M; Fraser M; Parent L; Vaillancourt C; Verreault J [Ad] Endereço: Centre de recherche en toxicologie de l'environnement (TOXEN), Département des sciences biologiques, Université du Québec à Montréal, C.P. 8888, succursale Centre-ville, Montreal, QC, H3C 3P8, Canada; Center for Interdisciplinary Research on Well-Being, Health, Society and Environment (CINBIOSE), Un [Ti] Título: Dechlorane Plus induces oxidative stress and decreases cyclooxygenase activity in the blue mussel. [So] Source: Aquat Toxicol;188:26-32, 2017 Jul. [Is] ISSN: 1879-1514 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Dechlorane Plus (DP) is a chlorinated flame retardant used mainly in electrical wire and cable coating, computer connectors, and plastic roofing materials. Concentrations of DP (syn and anti isomers) are increasingly being reported in aquatic ecosystems worldwide. However, there is exceedingly little information on the exposure-related toxicity of DP in aquatic organisms, especially in bivalves. The objective of this study was to investigate the in vivo and in vitro effects of DP exposure on histopathology, lipid peroxidation (LPO) levels, cyclooxygenase (COX) activity, phagocytosis capacity and efficiency, and DNA strand breakage in the blue mussel (Mytilus edulis) following a 29days exposure (0.001, 0.01, 0.1 and 1.0µg DP/L). Blue mussels accumulated DP in muscle and digestive gland in a dose-dependent manner. LPO levels in gills were found to increase by 82% and 67% at the 0.01 and 1.0µg DP/L doses, respectively, while COX activity in gills decreased by 44% at the 1µg/L dose. No histopathological lesion was found in gonads following DP exposure. Moreover, no change in hemocyte DNA strand breakage, phagocytosis rate, and viability was observed following DP exposure. Present study showed that toxicity of DP may occur primarily via oxidative stress in the blue mussel and potentially other bivalves, and that gills represent the most responsive tissue to this exposure. [Mh] Termos MeSH primário: Retardadores de Chama/toxicidade Hidrocarbonetos Clorados/toxicidade Mytilus edulis/efeitos dos fármacos Compostos Policíclicos/toxicidade Poluentes Químicos da Água/toxicidade [Mh] Termos MeSH secundário: Animais Dano ao DNA Trato Gastrointestinal/efeitos dos fármacos Trato Gastrointestinal/metabolismo Brânquias/efeitos dos fármacos Brânquias/metabolismo Peroxidação de Lipídeos/efeitos dos fármacos Músculos/efeitos dos fármacos Músculos/metabolismo Mytilus edulis/metabolismo Oxirredução Estresse Oxidativo/efeitos dos fármacos Prostaglandina-Endoperóxido Sintases/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Flame Retardants); 0 (Hydrocarbons, Chlorinated); 0 (Polycyclic Compounds); 0 (Water Pollutants, Chemical); 0 (dechlorane plus); EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171003 [Lr] Data última revisão: 171003 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170426 [St] Status: MEDLINE

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[PMID]: 28390017 [Au] Autor: Bourgeault A; Legros V; Gonnet F; Daniel R; Paquirissamy A; Bénatar C; Spalla O; Chanéac C; Renault JP; Pin S [Ad] Endereço: LIONS, NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay, 91191, Gif-sur-Yvette, France. adeline.bourgeault@edf.fr. [Ti] Título: Interaction of TiO nanoparticles with proteins from aquatic organisms: the case of gill mucus from blue mussel. [So] Source: Environ Sci Pollut Res Int;24(15):13474-13483, 2017 May. [Is] ISSN: 1614-7499 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: To better understand the mechanisms of TiO nanoparticle (NP) uptake and toxicity in aquatic organisms, we investigated the interaction of NPs with the proteins found in gill mucus from blue mussels. Mucus is secreted by many aquatic organisms and is often their first line of defense against pathogens, xenobiotics, and other sources of environmental stress. Here, five TiO NPs and one SiO NP were incubated with gill mucus and run out on a one-dimensional polyacrylamide gel for a comparative qualitative analysis of the free proteins in the mucosal solution and the proteins bound to NPs. We then used nanoscale liquid chromatography coupled with tandem mass spectrometry to identify proteins of interest. Our data demonstrated dissimilar protein profiles between the crude mucosal solution and proteins adsorbed on NPs. In particular, extrapallial protein (EP), one of the most abundant mucus proteins, was absent from the adsorbed proteins. After thermal denaturation experiments, this absence was attributed to the EP content in aromatic amino acids that prevents protein unfolding and thus adsorption on the NP. Moreover, although the majority of the protein corona was qualitatively similar across the NPs tested here (SiO and TiO ), a few proteins in the corona showed a specific recruitment pattern according to the NP oxide (TiO vs SiO ) or crystal structure (anatase TiO vs rutile TiO ). Therefore, protein adsorption may vary with the type of NP. Graphical abstract Proteins with adsorption selectivity as identified from isolated bands. [Mh] Termos MeSH primário: Brânquias Mytilus edulis [Mh] Termos MeSH secundário: Animais Organismos Aquáticos Muco Nanopartículas/química Dióxido de Silício Titânio/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 15FIX9V2JP (titanium dioxide); 7631-86-9 (Silicon Dioxide); D1JT611TNE (Titanium) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 171104 [Lr] Data última revisão: 171104 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170409 [St] Status: MEDLINE [do] DOI: 10.1007/s11356-017-8801-3

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[PMID]: 28371659 [Au] Autor: Jiang Y; Tang X; Zhou B; Sun T; Chen H; Zhao X; Wang Y [Ad] Endereço: Department of Marine Ecology, College of Marine Life Science, Ocean University of China, Qingdao, 266003, China. Electronic address: 1169753893@qq.com. [Ti] Título: The ROS-mediated pathway coupled with the MAPK-p38 signalling pathway and antioxidant system plays roles in the responses of Mytilus edulis haemocytes induced by BDE-47. [So] Source: Aquat Toxicol;187:55-63, 2017 Jun. [Is] ISSN: 1879-1514 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Our previous study found that BDE-47 could change the immune function of haemocytes in Mytilus edulis, and reactive oxygen species (ROS) might be involved in the process of physiological alteration. Here, we aimed to better understand this relationship. To accomplish this, we analysed changes in different ROS as well as various antioxidant system components. Additionally, the expression of MAPK-p38, a signalling protein regulated by ROS that helps to regulate numerous cellular processes, was also analysed. BDE-47 was given at low, medium, and high amounts. The results showed that (1) BDE-47 significantly affected ROS component levels in haemocytes. O content was increased under all conditions. H O content was also increased under all conditions, except in the middle concentration group. In contrast, OH content was increased in the low and middle concentration groups and decreased in the high concentration group. (2) Estimations of the antioxidant systems revealed concentration-dependent changes. Catalase activity was increased throughout the experiment, while superoxide dismutase (SOD) exhibited a decreasing trend in the tested groups with an increase of exposure time. On day 21, only the high concentration group showed a slight increase in SOD activity compared to the control. Furthermore, glutathione peroxidase and glutathione reductase activity increased in the low and middle concentration groups but decreased in the high concentration group. The GSH/GSSG ratio increased for all treatments over time, indicating that changes in redox status occurred. (3) MAPK-p38 was activated following BDE-47 exposure. Based on our previous study, we speculate that BDE-47 exposure induces ROS production and affects the ROS-mediated pathway, which may explain the resultant functional damage observed in haemocytes. Furthermore, BDE-47 also affected the antioxidant system and altered redox status, although these changes did not ameliorate the damage caused by ROS. [Mh] Termos MeSH primário: Antioxidantes/metabolismo Éteres Difenil Halogenados/toxicidade Hemócitos/efeitos dos fármacos Sistema de Sinalização das MAP Quinases/efeitos dos fármacos Mytilus edulis/efeitos dos fármacos Espécies Reativas de Oxigênio/metabolismo Poluentes Químicos da Água/toxicidade [Mh] Termos MeSH secundário: Animais Catalase/metabolismo Glutationa/metabolismo Glutationa Peroxidase/metabolismo Hemócitos/metabolismo Peróxido de Hidrogênio/metabolismo Mytilus edulis/metabolismo Oxirredução Estresse Oxidativo/efeitos dos fármacos Superóxido Dismutase/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Halogenated Diphenyl Ethers); 0 (Reactive Oxygen Species); 0 (Water Pollutants, Chemical); 0N97R5X10X (2,2',4,4'-tetrabromodiphenyl ether); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); GAN16C9B8O (Glutathione) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170907 [Lr] Data última revisão: 170907 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170404 [St] Status: MEDLINE

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MEDLINE

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[PMID]: 28334251 [Au] Autor: Eggermont M; Bossier P; Pande GSJ; Delahaut V; Rayhan AM; Gupta N; Islam SS; Yumo E; Nevejan N; Sorgeloos P; Gomez-Gil B; Defoirdt T [Ad] Endereço: Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium. [Ti] Título: Isolation of Vibrionaceae from wild blue mussel (Mytilus edulis) adults and their impact on blue mussel larviculture. [So] Source: FEMS Microbiol Ecol;93(4), 2017 Apr 01. [Is] ISSN: 1574-6941 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The blue mussel (Mytilus edulis) is known as a robust bivalve species, although its larviculture appears to be highly susceptible to diseases. In this study, we isolated 17 strains from induced mortality events in healthy wild-caught blue mussel adults and demonstrated that they caused between 17% and 98% mortality in blue mussel larvae in a newly developed, highly controlled immersion challenge test model. Eight of the isolates belong to the Splendidus clade of vibrios, while the other isolates belong to the genus Photobacterium. The genomes of the most virulent Vibrio isolate and the most virulent Photobacterium isolate were sequenced and contained several genes encoding factors that have previously been linked to virulence towards bivalves. In vitro tests confirmed that all 17 isolates were positive for these virulence factors. The sequenced genomes also contained a remarkably high number of multidrug resistance genes. We therefore assessed the sensitivity of all isolates to a broad range of antibiotics and found that there were indeed many strong positive correlations between the sensitivities of the isolates to different antibiotics. Our data provide an ecological insight into mass mortality in blue mussels as they indicate that wild mussels contain a reservoir of pathogenic bacteria. [Mh] Termos MeSH primário: Mytilus edulis/microbiologia Vibrionaceae/isolamento & purificação [Mh] Termos MeSH secundário: Animais Aquicultura Vibrio Vibrionaceae/crescimento & desenvolvimento [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170620 [Lr] Data última revisão: 170620 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170324 [St] Status: MEDLINE [do] DOI: 10.1093/femsec/fix039

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