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[PMID]:29080422
[Au] Autor:Sakata J; Yonekita T; Kawatsu K
[Ad] Endereço:Division of Microbiology Bacteriology Section, Osaka Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan. Electronic address: sakata@iph.osaka.jp.
[Ti] Título:Development of a rapid immunochromatographic assay to detect contamination of raw oysters with enteropathogenic Vibrio parahaemolyticus.
[So] Source:Int J Food Microbiol;264:16-24, 2018 Jan 02.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) are major virulence factors of enteropathogenic Vibrio parahaemolyticus. TDH and TRH are bacterial exotoxins, and their presence in culture medium serves as a specific marker for detecting this significant pathogen. Here, we developed and evaluated an immunochromatographic assay (TDH/TRH-ICA) to simultaneously or individually detect TDH and TRH. The TDH/TRH-ICA detected TDH in all broth cultures of 47 V. parahaemolyticus strains carrying tdh. The genes encoding TRH are classified as variants trh1 and trh2, and TRH was detected in all broth cultures of 25 V. parahaemolyticus strains carrying trh1 and certain proportion (5/31) of broth cultures of V. parahaemolyticus strains carrying trh2. In contrast, TDH and TRH were not detected in broth cultures of 12 non-enteropathogenic V. parahaemolyticus strains without tdh and trh. It was difficult to detect TRH2 using the TDH/TRH-ICA. However, TRH2 may not serve as a suitable marker for detecting enteropathogenic V. parahaemolyticus, and evidence indicates that TRH2 may not contribute to enteropathogenesis. Further, a screening method using a combination of TDH/TRH-ICA and SPP medium supplemented with 1.5% NaCl (modified-SPP medium) detected oyster samples artificially spiked with 1.1-22 colony-forming units of enteropathogenic V. parahaemolyticus per 25g of oysters within approximately 8.5h, including the enrichment culture. The assay may serve as a method that facilitates the rapid and easy detection of raw oysters contaminated with enteropathogenic V. parahaemolyticus.
[Mh] Termos MeSH primário: Proteínas de Bactérias/análise
Proteínas Hemolisinas/análise
Imunocromatografia/métodos
Ostreidae/microbiologia
Vibrio parahaemolyticus/genética
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/imunologia
Proteínas de Bactérias/genética
Proteínas de Bactérias/imunologia
Toxinas Bacterianas/análise
Toxinas Bacterianas/genética
Toxinas Bacterianas/imunologia
Proteínas Hemolisinas/genética
Proteínas Hemolisinas/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Vibrio parahaemolyticus/classificação
Vibrio parahaemolyticus/isolamento & purificação
Fatores de Virulência/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Bacterial Proteins); 0 (Bacterial Toxins); 0 (Hemolysin Proteins); 0 (Virulence Factors); 0 (thermostable direct hemolysin-related hemolysin protein, Vibrio parahaemolyticus); 135433-21-5 (thermostable direct hemolysin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171029
[St] Status:MEDLINE


  2 / 2710 MEDLINE  
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[PMID]:28747477
[Au] Autor:Fodrie FJ; Rodriguez AB; Gittman RK; Grabowski JH; Lindquist NL; Peterson CH; Piehler MF; Ridge JT
[Ad] Endereço:Institute of Marine Sciences, University of North Carolina at Chapel Hill, 3431 Arendell Street, Morehead City, NC 28557, USA jfodrie@unc.edu.
[Ti] Título:Oyster reefs as carbon sources and sinks.
[So] Source:Proc Biol Sci;284(1859), 2017 Jul 26.
[Is] ISSN:1471-2954
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Carbon burial is increasingly valued as a service provided by threatened vegetated coastal habitats. Similarly, shellfish reefs contain significant pools of carbon and are globally endangered, yet considerable uncertainty remains regarding shellfish reefs' role as sources (+) or sinks (-) of atmospheric CO While CO release is a by-product of carbonate shell production (then burial), shellfish also facilitate atmospheric-CO drawdown via filtration and rapid biodeposition of carbon-fixing primary producers. We provide a framework to account for the dual burial of inorganic and organic carbon, and demonstrate that decade-old experimental reefs on intertidal sandflats were net sources of CO (7.1 ± 1.2 MgC ha yr (µ ± s.e.)) resulting from predominantly carbonate deposition, whereas shallow subtidal reefs (-1.0 ± 0.4 MgC ha yr ) and saltmarsh-fringing reefs (-1.3 ± 0.4 MgC ha yr ) were dominated by organic-carbon-rich sediments and functioned as net carbon sinks (on par with vegetated coastal habitats). These landscape-level differences reflect gradients in shellfish growth, survivorship and shell bioerosion. Notably, down-core carbon concentrations in 100- to 4000-year-old reefs mirrored experimental-reef data, suggesting our results are relevant over centennial to millennial scales, although we note that these natural reefs appeared to function as slight carbon sources (0.5 ± 0.3 MgC ha yr ). Globally, the historical mining of the top metre of shellfish reefs may have reintroduced more than 400 000 000 Mg of organic carbon into estuaries. Importantly, reef formation and destruction do not have reciprocal, counterbalancing impacts on atmospheric CO since excavated organic material may be remineralized while shell may experience continued preservation through reburial. Thus, protection of existing reefs could be considered as one component of climate mitigation programmes focused on the coastal zone.
[Mh] Termos MeSH primário: Sequestro de Carbono
Ecossistema
Ostreidae
[Mh] Termos MeSH secundário: Animais
Carbono/química
Dióxido de Carbono/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
142M471B3J (Carbon Dioxide); 7440-44-0 (Carbon)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE


  3 / 2710 MEDLINE  
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[PMID]:28859689
[Au] Autor:Konrad S; Paduraru P; Romero-Barrios P; Henderson SB; Galanis E
[Ad] Endereço:Canadian Field Epidemiology Program, Public Health Agency of Canada, 130 Colonnade Road, Ottawa, Ontario, K1A 0K9, Canada.
[Ti] Título:Remote sensing measurements of sea surface temperature as an indicator of Vibrio parahaemolyticus in oyster meat and human illnesses.
[So] Source:Environ Health;16(1):92, 2017 Aug 31.
[Is] ISSN:1476-069X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Vibrio parahaemolyticus (Vp) is a naturally occurring bacterium found in marine environments worldwide. It can cause gastrointestinal illness in humans, primarily through raw oyster consumption. Water temperatures, and potentially other environmental factors, play an important role in the growth and proliferation of Vp in the environment. Quantifying the relationships between environmental variables and indicators or incidence of Vp illness is valuable for public health surveillance to inform and enable suitable preventative measures. This study aimed to assess the relationship between environmental parameters and Vp in British Columbia (BC), Canada. METHODS: The study used Vp counts in oyster meat from 2002-2015 and laboratory confirmed Vp illnesses from 2011-2015 for the province of BC. The data were matched to environmental parameters from publicly available sources, including remote sensing measurements of nighttime sea surface temperature (SST) obtained from satellite readings at a spatial resolution of 1 km. Using three separate models, this paper assessed the relationship between (1) daily SST and Vp counts in oyster meat, (2) weekly mean Vp counts in oysters and weekly Vp illnesses, and (3) weekly mean SST and weekly Vp illnesses. The effects of salinity and chlorophyll a were also evaluated. Linear regression was used to quantify the relationship between SST and Vp, and piecewise regression was used to identify SST thresholds of concern. RESULTS: A total of 2327 oyster samples and 293 laboratory confirmed illnesses were included. In model 1, both SST and salinity were significant predictors of log(Vp) counts in oyster meat. In model 2, the mean log(Vp) count in oyster meat was a significant predictor of Vp illnesses. In model 3, weekly mean SST was a significant predictor of weekly Vp illnesses. The piecewise regression models identified a SST threshold of approximately 14 C for both model 1 and 3, indicating increased risk of Vp in oyster meat and Vp illnesses at higher temperatures. CONCLUSION: Monitoring of SST, particularly through readily accessible remote sensing data, could serve as a warning signal for Vp and help inform the introduction and cessation of preventative or control measures.
[Mh] Termos MeSH primário: Microbiologia de Alimentos/métodos
Doenças Transmitidas por Alimentos/epidemiologia
Ostreidae/microbiologia
Tecnologia de Sensoriamento Remoto/utilização
Alimentos Marinhos/microbiologia
Vibrioses/epidemiologia
Vibrio parahaemolyticus/fisiologia
[Mh] Termos MeSH secundário: Animais
Colúmbia Britânica/epidemiologia
Doenças Transmitidas por Alimentos/microbiologia
Seres Humanos
Incidência
Oceano Pacífico
Água do Mar/química
Temperatura Ambiente
Vibrioses/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170902
[St] Status:MEDLINE
[do] DOI:10.1186/s12940-017-0301-x


  4 / 2710 MEDLINE  
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[PMID]:28810227
[Au] Autor:Campos CJA; Goblick G; Lee R; Wittamore K; Lees DN
[Ad] Endereço:Centre for Environment, Fisheries and Aquaculture Science (Cefas), Weymouth Laboratory, Weymouth DT48UB, UK. Electronic address: carlos.campos@cefas.co.uk.
[Ti] Título:Determining the zone of impact of norovirus contamination in shellfish production areas through microbiological monitoring and hydrographic analysis.
[So] Source:Water Res;124:556-565, 2017 Nov 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Norovirus (NoV) contamination of filter feeding bivalve shellfish is a well-recognised human health threat when shellfish are grown in sewage polluted waters. To date, the identification of high risk zones around sewage discharges in shellfish production areas (SPAs) has not been based on NoV data. This study utilised molecular methods for NoV analysis, combined with hydrographic studies, to determine the relationship between NoV concentrations in shellfish and sewage effluent dilution. Cages with mussels and oysters were placed at different distances downstream of sewage discharges in two coastal sites in England. The shellfish were tested for concentrations of NoV (genogroups I and II) and E. coli. Drogue tracking and dye tracing studies were conducted to quantify the dispersion and dilution of sewage effluent in the SPAs. Significant negative associations were found between both total concentrations of NoV (GI + GII) and E. coli and sewage effluent dilution in the SPAs. The total NoV concentrations predicted by the model at 300:1, 1000:1 and 5000:1 ratios of estuarine water to sewage effluent were 1200; 600; and 200 copies/g, respectively. The estimated area of NoV contamination varied according with local pollution source impacts and hydrographic characteristics. The results help to inform the derivation of sewage discharge buffer zones as a control measure for mitigating risk from human NoV contamination in SPAs.
[Mh] Termos MeSH primário: Norovirus
Frutos do Mar/virologia
[Mh] Termos MeSH secundário: Animais
Inglaterra
Escherichia coli
Seres Humanos
Ostreidae
Esgotos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sewage)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE


  5 / 2710 MEDLINE  
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[PMID]:28792543
[Au] Autor:Ricci SW; Bohnenstiehl DR; Eggleston DB; Kellogg ML; Lyon RP
[Ad] Endereço:Department of Marine, Earth and Atmospheric Sciences, North Carolina State University, Raleigh, North Carolina, United States of America.
[Ti] Título:Oyster toadfish (Opsanus tau) boatwhistle call detection and patterns within a large-scale oyster restoration site.
[So] Source:PLoS One;12(8):e0182757, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During May 2015, passive acoustic recorders were deployed at eight subtidal oyster reefs within Harris Creek Oyster Sanctuary in Chesapeake Bay, Maryland USA. These sites were selected to represent both restored and unrestored habitats having a range of oyster densities. Throughout the survey, the soundscape within Harris Creek was dominated by the boatwhistle calls of the oyster toadfish, Opsanus tau. A novel, multi-kernel spectral correlation approach was developed to automatically detect these boatwhistle calls using their two lowest harmonic bands. The results provided quantitative information on how call rate and call frequency varied in space and time. Toadfish boatwhistle fundamental frequency ranged from 140 Hz to 260 Hz and was well correlated (r = 0.94) with changes in water temperature, with the fundamental frequency increasing by ~11 Hz for every 1°C increase in temperature. The boatwhistle call rate increased from just a few calls per minute at the start of monitoring on May 7th to ~100 calls/min on May 10th and remained elevated throughout the survey. As male toadfish are known to generate boatwhistles to attract mates, this rapid increase in call rate was interpreted to mark the onset of spring spawning behavior. Call rate was not modulated by water temperature, but showed a consistent diurnal pattern, with a sharp decrease in rate just before sunrise and a peak just after sunset. There was a significant difference in call rate between restored and unrestored reefs, with restored sites having nearly twice the call rate as unrestored sites. This work highlights the benefits of using automated detection techniques that provide quantitative information on species-specific call characteristics and patterns. This type of non-invasive acoustic monitoring provides long-term, semi-continuous information on animal behavior and abundance, and operates effectively in settings that are otherwise difficult to sample.
[Mh] Termos MeSH primário: Batracoidiformes
Vocalização Animal
[Mh] Termos MeSH secundário: Animais
Relógios Circadianos
Conservação dos Recursos Naturais
Ecossistema
Maryland
Ostreidae
Reconhecimento Automatizado de Padrão
Fotoperíodo
Água do Mar
Espectrografia do Som
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182757


  6 / 2710 MEDLINE  
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[PMID]:28780263
[Au] Autor:Tong S; Stocks JL; Rodriguez-Gonzalez LC; Feng C; Ergas SJ
[Ad] Endereço:School of Water Resources and Environment, China University of Geosciences Beijing, Beijing 100083, China; Department of Civil and Environmental Engineering, University of South Florida, 4202 E. Fowler Ave, ENB 118, Tampa, FL 33620, USA.
[Ti] Título:Effect of oyster shell medium and organic substrate on the performance of a particulate pyrite autotrophic denitrification (PPAD) process.
[So] Source:Bioresour Technol;244(Pt 1):296-303, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The use of pyrite as an electron donor for biological denitrification has the potential to reduce alkalinity consumption and sulfate by-product production compared with sulfur oxidizing denitrification. This research investigated the effects of oyster shell and organic substrate addition on the performance of a particulate pyrite autotrophic denitrification (PPAD) process. Side-by-side bench-scale studies were carried out in upflow packed bed bioreactors with pyrite and sand, with and without oyster shells as an alkalinity source. Organic carbon addition (10% by volume wastewater) was found to improve PPAD denitrification performance, possibly by promoting mixotrophic metabolism. After organic carbon addition and operation at a six-hour empty bed contact time, total inorganic nitrogen (TIN) removal reached 90% in the column with oyster shells compared with 70% without. SEM images and biofilm protein measurements indicated that oyster shells enhanced biofilm growth. The results indicate that PPAD is a promising technology for treatment of nitrified wastewater.
[Mh] Termos MeSH primário: Reatores Biológicos
Desnitrificação
Ostreidae
[Mh] Termos MeSH secundário: Animais
Processos Autotróficos
Ferro
Nitratos
Sulfetos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrates); 0 (Sulfides); 132N09W4PR (pyrite); E1UOL152H7 (Iron)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170807
[St] Status:MEDLINE


  7 / 2710 MEDLINE  
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[PMID]:28696147
[Au] Autor:Grodeska SM; Jones JL; Arias CR; Walton WC
[Ad] Endereço:1 Auburn University Shellfish Laboratory, School of Fisheries, Aquaculture & Aquatic Sciences, Auburn University, 150 Agassiz Street, Dauphin Island, Alabama 36528; and.
[Ti] Título:Effects of Desiccation Practices of Cultured Atlantic Oysters (Crassostrea virginica) on Vibrio spp. in Portersville Bay, Alabama, USA.
[So] Source:J Food Prot;80(8):1280-1287, 2017 Aug.
[Is] ISSN:1944-9097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The expansion of off-bottom aquaculture to the Gulf of Mexico has raised public health concerns for human health officials. High temperatures in the Gulf of Mexico are associated with high levels of Vibrio parahaemolyticus and Vibrio vulnificus. Routine desiccation practices associated with off-bottom aquaculture expose oysters to ambient air, allowing Vibrio spp. to proliferate in the closed oyster. Currently, there is limited research on the length of time needed for Vibrio spp. levels in desiccated oysters to return to background levels, defined as the levels found in oysters that remain continually submersed and not exposed to ambient air. This study determined the time needed to return V. parahaemolyticus, V. vulnificus, and Vibrio cholerae levels to background levels in oysters exposed to the following desiccation practices: 3-h freshwater dip followed by 24-h ambient air exposure, 27-h ambient air exposure, and control. All oysters were submerged at least 2 weeks prior to the beginning of each trial, with the control samples remaining submerged for the duration of each trial. Vibrio spp. levels were enumerated from samples collected on days 0, 1, 2, 3, 7, 10, and 14 after resubmersion using a three-tube most-probable-number enrichment followed by BAX PCR. V. cholerae levels were frequently (92%) below the limit of detection at all times, so they were not statistically analyzed. V. parahaemolyticus and V. vulnificus levels in the 27-h ambient air exposure and the 3-h freshwater dip followed by 24-h ambient air exposure samples were significantly elevated compared with background samples. In most cases, the Vibrio spp. levels in oysters in both desiccation treatments remained elevated compared with background levels until 2 or 3 days post-resubmersion. However, there was one trial in which the Vibrio spp. levels did not return to background levels until day 7. The results of this study provide scientific support that oyster farmers should be required to implement a minimum 7-day resubmersion regimen. This length of time allowed the Vibrio spp. levels to become not significantly different across all treatments.
[Mh] Termos MeSH primário: Crassostrea/microbiologia
Dessecação
Contaminação de Alimentos/prevenção & controle
Vibrio parahaemolyticus/crescimento & desenvolvimento
Vibrio vulnificus/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Alabama
Animais
Baías
Contagem de Colônia Microbiana
Contaminação de Alimentos/análise
Seres Humanos
Ostreidae
Frutos do Mar/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-16-297


  8 / 2710 MEDLINE  
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[PMID]:28695254
[Au] Autor:Loh A; Yim UH; Ha SY; An JG; Kim M
[Ad] Endereço:Oil and POPs Research Group, South Sea Research Institute, KIOST, Geoje, 53201, Republic of Korea.
[Ti] Título:Contamination and Human Health Risk Assessment of Polycyclic Aromatic Hydrocarbons (PAHs) in Oysters After the Wu Yi San Oil Spill in Korea.
[So] Source:Arch Environ Contam Toxicol;73(1):103-117, 2017 Jul.
[Is] ISSN:1432-0703
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:After the collision of the Singapore-registered oil tanker M/V Wu Yi San into the oil terminal of Yeosu, Korea on January 31, 2014, approximately 900 m of oil and oil mixture were released from the ruptured pipelines. The oil affected more than 10 km of coastline along Gwangyang Bay. Emergency oil spill responses recovered bulk oil at sea and cleaned up the stranded oil on shore. As part of an emergency environmental impact assessment, region-wide monitoring of oil contamination in oyster had been conducted for 2 months. Highly elevated concentrations of polycyclic aromatic hydrocarbons (PAHs) were detected at most of the spill affected sites. Four days after the spill, the levels of PAHs in oysters increased dramatically to 627-81,000 ng/g, the average of which was 20 times higher than those found before the spill (321-4040 ng/g). The level of PAHs in these oysters increased until 10 days after the spill and then decreased. Due to the strong tidal current and easterly winter winds, the eastern part of the Bay-the Namhae region-was heavily contaminated compared with other regions. The accumulation and depuration of spilled oil in oyster corresponded with the duration and intensity of the cleanup activities, which is the first field observation in oil spill cases. Human health risk assessments showed that benzo[a]pyrene equivalent concentrations exceeded levels of concern in the highly contaminated sites, even 60 days after the spill.
[Mh] Termos MeSH primário: Dieta/estatística & dados numéricos
Exposição Ambiental/estatística & dados numéricos
Ostreidae/metabolismo
Poluição por Petróleo/análise
Hidrocarbonetos Aromáticos Policíclicos/metabolismo
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Poluição por Petróleo/estatística & dados numéricos
República da Coreia
Medição de Risco
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polycyclic Aromatic Hydrocarbons); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.1007/s00244-017-0394-9


  9 / 2710 MEDLINE  
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[PMID]:28594821
[Au] Autor:Riviere G; He Y; Tecchio S; Crowell E; Gras M; Sourdaine P; Guo X; Favrel P
[Ad] Endereço:Normandy University, Caen, France.
[Ti] Título:Dynamics of DNA methylomes underlie oyster development.
[So] Source:PLoS Genet;13(6):e1006807, 2017 Jun.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA methylation is a critical epigenetic regulator of development in mammals and social insects, but its significance in development outside these groups is not understood. Here we investigated the genome-wide dynamics of DNA methylation in a mollusc model, the oyster Crassostrea gigas, from the egg to the completion of organogenesis. Large-scale methylation maps reveal that the oyster genome displays a succession of methylated and non methylated regions, which persist throughout development. Differentially methylated regions (DMRs) are strongly regulated during cleavage and metamorphosis. The distribution and levels of methylated DNA within genomic features (exons, introns, promoters, repeats and transposons) show different developmental lansdscapes marked by a strong increase in the methylation of exons against introns after metamorphosis. Kinetics of methylation in gene-bodies correlate to their transcription regulation and to distinct functional gene clusters, and DMRs at cleavage and metamorphosis bear the genes functionally related to these steps, respectively. This study shows that DNA methylome dynamics underlie development through transcription regulation in the oyster, a lophotrochozoan species. To our knowledge, this is the first demonstration of such epigenetic regulation outside vertebrates and ecdysozoan models, bringing new insights into the evolution and the epigenetic regulation of developmental processes.
[Mh] Termos MeSH primário: Metilação de DNA
Regulação da Expressão Gênica no Desenvolvimento
Ostreidae/genética
[Mh] Termos MeSH secundário: Animais
Genoma
Ostreidae/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006807


  10 / 2710 MEDLINE  
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[PMID]:28570710
[Au] Autor:Zheng Z; Du X; Xiong X; Jiao Y; Deng Y; Wang Q; Huang R
[Ad] Endereço:Fishery College, Guangdong Ocean University, Zhanjiang, China.
[Ti] Título:PmRunt regulated by Pm-miR-183 participates in nacre formation possibly through promoting the expression of collagen VI-like and Nacrein in pearl oyster Pinctada martensii.
[So] Source:PLoS One;12(6):e0178561, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Heterodimeric PEBP2/CBFs are key regulators in diverse biological processes, such as haematopoietic stem-cell generation, bone formation and cancers. In this work, we cloned runt-like transcriptional factor (designated as PmRunt) and CBF ß (designated as PmCBF) gene, which comprise the heterodimeric transcriptional factor in Pinctada martensii. PmRunt was identified with an open reading frame that encodes 545 amino acids and has typical Runt domain. Phylogenetic analysis results speculated that runt-like transcriptional factors (RDs) in vertebrates and invertebrates are separated into two branches. In molluscs, PmRunt and other RDs are clustered in one of these branches. Direct interaction between PmRunt and PmCBF was evidenced by yeast two-hybrid assay results. Gene repression by RNA interference decreased the expression level of PmRunt, and subsequent observation of the inner surface of the nacre by scanning electron microscopy demonstrated disordered growth. The luciferase activities of reporters that contain promoter regions of Collagen VI-like (PmColVI) and PmNacrein were enhanced by PmRunt. Meanwhile, Pm-miR-183 apparently inhibited the relative luciferase activity of reporters containing the 3'-UTR of PmRunt. The expression level of PmRunt was repressed after Pm-miR-183 was overexpressed in the mantle tissue. Therefore, we proposed that PmRunt could be targeted by Pm-miR-183 and regulate the transcription of PmColVI and PmNacrein by increasing their transcriptional activity, thereby governing nacre formation.
[Mh] Termos MeSH primário: Anidrases Carbônicas/metabolismo
Colágeno Tipo VI/metabolismo
MicroRNAs/genética
Proteínas/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Ostreidae
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Collagen Type VI); 0 (MicroRNAs); 0 (Proteins); EC 4.2.1.- (nacrein); EC 4.2.1.1 (Carbonic Anhydrases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178561



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