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Pesquisa : B01.050.500.644.080.643.600 [Categoria DeCS]
Referências encontradas : 86 [refinar]
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[PMID]:29256422
[Au] Autor:Suong NT; Webb S; Banks J; Wakeman KC; Lane H; Jeffs A; Brosnahan C; Jones B; Fidler A
[Ad] Endereço:Institute of Marine Science, University of Auckland, Auckland 1142, New Zealand.
[Ti] Título:Partial 18S rRNA sequences of apicomplexan parasite 'X' (APX), associated with flat oysters Ostrea chilensis in New Zealand.
[So] Source:Dis Aquat Organ;127(1):1-9, 2017 Dec 19.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Apicomplexa is a large phylum of parasitic protists renowned for significant negative health impacts on humans and livestock worldwide. Despite the prevalence and negative impacts of apicomplexans across many animal groups, relatively little attention has been given to apicomplexan parasites of invertebrates, especially marine invertebrates. Previous work has reported an apicomplexan parasite 'X' (APX), a parasite that has been histologically and ultrastructurally identified from the commercially important flat oyster Ostrea chilensis in New Zealand. This apicomplexan may exacerbate host vulnerability to the infectious disease bonamiosis. In this study, we report 18S rRNA sequences amplified from APX-infected O. chilensis tissues. Phylogenetic analyses clearly established that the 18S sequences were of apicomplexan origin; however, their detailed relationship to known apicomplexan groups is less resolved. Two specific probes, designed from the putative APX 18S rRNA sequence, co-localised with APX cells in in situ hybridisations, further supporting our hypothesis that the 18S sequences were from APX. These sequences will facilitate the future development of inexpensive and sensitive molecular diagnostic tests for APX, thereby assisting research focussed on the biology and ecology of this organism and its role in morbidity and mortality of O. chilensis.
[Mh] Termos MeSH primário: Apicomplexa/classificação
Apicomplexa/genética
Ostrea/parasitologia
RNA Ribossômico 18S/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Nova Zelândia
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.3354/dao03175


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[PMID]:27815201
[Au] Autor:Pardo BG; Álvarez-Dios JA; Cao A; Ramilo A; Gómez-Tato A; Planas JV; Villalba A; Martínez P
[Ad] Endereço:Departamento de Zoología, Genética y Antropología Física, Facultad de Veterinaria, Universidade de Santiago de Compostela, Campus de Lugo, 27002 Lugo, Spain. Electronic address: belen.gomez@usc.es.
[Ti] Título:Construction of an Ostrea edulis database from genomic and expressed sequence tags (ESTs) obtained from Bonamia ostreae infected haemocytes: Development of an immune-enriched oligo-microarray.
[So] Source:Fish Shellfish Immunol;59:331-344, 2016 Dec.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The flat oyster, Ostrea edulis, is one of the main farmed oysters, not only in Europe but also in the United States and Canada. Bonamiosis due to the parasite Bonamia ostreae has been associated with high mortality episodes in this species. This parasite is an intracellular protozoan that infects haemocytes, the main cells involved in oyster defence. Due to the economical and ecological importance of flat oyster, genomic data are badly needed for genetic improvement of the species, but they are still very scarce. The objective of this study is to develop a sequence database, OedulisDB, with new genomic and transcriptomic resources, providing new data and convenient tools to improve our knowledge of the oyster's immune mechanisms. Transcriptomic and genomic sequences were obtained using 454 pyrosequencing and compiled into an O. edulis database, OedulisDB, consisting of two sets of 10,318 and 7159 unique sequences that represent the oyster's genome (WG) and de novo haemocyte transcriptome (HT), respectively. The flat oyster transcriptome was obtained from two strains (naïve and tolerant) challenged with B. ostreae, and from their corresponding non-challenged controls. Approximately 78.5% of 5619 HT unique sequences were successfully annotated by Blast search using public databases. A total of 984 sequences were identified as being related to immune response and several key immune genes were identified for the first time in flat oyster. Additionally, transcriptome information was used to design and validate the first oligo-microarray in flat oyster enriched with immune sequences from haemocytes. Our transcriptomic and genomic sequencing and subsequent annotation have largely increased the scarce resources available for this economically important species and have enabled us to develop an OedulisDB database and accompanying tools for gene expression analysis. This study represents the first attempt to characterize in depth the O. edulis haemocyte transcriptome in response to B. ostreae through massively sequencing and has aided to improve our knowledge of the immune mechanisms of flat oyster. The validated oligo-microarray and the establishment of a reference transcriptome will be useful for large-scale gene expression studies in this species.
[Mh] Termos MeSH primário: Bases de Dados Genéticas
Genoma
Haplosporídios/imunologia
Imunidade Inata
Análise de Sequência com Séries de Oligonucleotídeos
Ostrea/genética
Ostrea/parasitologia
[Mh] Termos MeSH secundário: Animais
Etiquetas de Sequências Expressas
Hemócitos/imunologia
Hemócitos/metabolismo
Hemócitos/parasitologia
Ostrea/imunologia
Análise de Sequência de DNA
Análise de Sequência de RNA
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


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[PMID]:27431587
[Au] Autor:Gervais O; Chollet B; Renault T; Arzul I
[Ad] Endereço:Ifremer, RBE-SG2M-LGPMM, Station de La Tremblade, Avenue de Mus de Loup, F-17390, La Tremblade, France.
[Ti] Título:Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae.
[So] Source:Fish Shellfish Immunol;56:322-329, 2016 Sep.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses. Whatever the host species was, the parasite was engulfed in hemocytes and induced an increase of apoptotic parameters including intracytoplasmic calcium concentration, mitochondrial membrane potential or phosphatidyl-serine externalization as well as ultrastructural modifications. However, the parasite appears more able to infect flat oyster than cupped oyster hemocytes and the apoptotic response was more important against live than dead parasites in the natural host than in C. gigas. Our results suggest that O. edulis specifically responds to B. ostreae by inducing apoptosis of hemocytes.
[Mh] Termos MeSH primário: Apoptose
Haplosporídios/fisiologia
Interações Hospedeiro-Parasita
Ostrea/fisiologia
Ostrea/parasitologia
[Mh] Termos MeSH secundário: Animais
Citometria de Fluxo
Hemócitos/parasitologia
Hemócitos/fisiologia
Hemócitos/ultraestrutura
Microscopia Eletrônica de Transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170812
[Lr] Data última revisão:
170812
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160720
[St] Status:MEDLINE


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[PMID]:27377003
[Au] Autor:Zwerschke N; Emmerson MC; Roberts D; O'Connor NE
[Ad] Endereço:Queen's University Belfast Marine Laboratory, 12-13 The Strand, Portaferry BT22 1PF, Northern Ireland, United Kingdom. Electronic address: nzwerschke01@qub.ac.uk.
[Ti] Título:Benthic assemblages associated with native and non-native oysters are similar.
[So] Source:Mar Pollut Bull;111(1-2):305-310, 2016 Oct 15.
[Is] ISSN:1879-3363
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Invasive species can impact native species and alter assemblage structure, which affects associated ecosystem functioning. The pervasive Pacific oyster, Crassostrea gigas, has been shown to affect the diversity and composition of many host ecosystems. We tested for effects of the presence of the invasive C. gigas on native assemblages by comparing them directly to assemblages associated with the declining native European oyster, Ostrea edulis. The presence of both oyster species was manipulated in intertidal and subtidal habitats and reefs were constructed at horizontal and vertical orientation to the substratum. After 12months, species diversity and benthic assemblage structure between assemblages with C. gigas and O. edulis were similar, but differed between habitats and orientation, suggesting that both oyster species were functionally similar in terms of biodiversity facilitation. These findings support evidence, that non-native species could play an important role in maintaining biodiversity in systems with declining populations of native species.
[Mh] Termos MeSH primário: Biodiversidade
Crassostrea
Espécies Introduzidas
Ostrea
[Mh] Termos MeSH secundário: Animais
Ecossistema
Irlanda
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160706
[St] Status:MEDLINE


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[PMID]:27239693
[Au] Autor:Green DS
[Ad] Endereço:School of Life Sciences, Gibbet Hill Campus, The University of Warwick, Coventry, United Kingdom; Biogeochemistry Research Group, Geography Department, School of Natural Sciences, Trinity College Dublin, Dublin, Ireland; Queens University Belfast, Marine Laboratory, Portaferry, Northern Ireland, United Kingdom. Electronic address: danniellesgreen@gmail.com.
[Ti] Título:Effects of microplastics on European flat oysters, Ostrea edulis and their associated benthic communities.
[So] Source:Environ Pollut;216:95-103, 2016 Sep.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plastic pollution is recognised as an emerging threat to aquatic ecosystems, with microplastics now the most abundant type of marine debris. Health effects caused by microplastics have been demonstrated at the species level, but impacts on ecological communities remain unknown. In this study, impacts of microplastics on the health and biological functioning of European flat oysters (Ostrea edulis) and on the structure of associated macrofaunal assemblages were assessed in an outdoor mesocosm experiment using intact sediment cores. Biodegradable and conventional microplastics were added at low (0.8 µg L(-1)) and high (80 µg L(-1)) doses in the water column repeatedly for 60 days. Effects on the oysters were minimal, but benthic assemblage structures differed and species richness and the total number of organisms were ∼1.2 and 1.5 times greater in control mesocosms than in those exposed to high doses of microplastics. Notably, abundances of juvenile Littorina sp. (periwinkles) and Idotea balthica (an isopod) were ∼2 and 8 times greater in controls than in mesocosms with the high dose of either type of microplastic. In addition, the biomass of Scrobicularia plana (peppery furrow shell clam) was ∼1.5 times greater in controls than in mesocosms with the high dose of microplastics. This work indicates that repeated exposure to high concentrations of microplastics could alter assemblages in an important marine habitat by reducing the abundance of benthic fauna.
[Mh] Termos MeSH primário: Ecossistema
Ostrea/efeitos dos fármacos
Plásticos/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Biodiversidade
Biomassa
Bivalves/efeitos dos fármacos
Bivalves/fisiologia
Exposição Ambiental/efeitos adversos
Seres Humanos
Isópodes/fisiologia
Ostrea/fisiologia
Plásticos/análise
Água do Mar/química
Testes de Toxicidade
Vinca/efeitos dos fármacos
Vinca/fisiologia
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plastics); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170118
[Lr] Data última revisão:
170118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160531
[St] Status:MEDLINE


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[PMID]:26880159
[Au] Autor:Flannery G; Lynch SA; Culloty SC
[Ad] Endereço:Aquaculture and Fisheries Development Centre, School of Biological, Earth and Environmental Sciences, University College Cork, Ireland. Electronic address: grace.flannery@umail.ucc.ie.
[Ti] Título:Investigating the significance of the role of Ostrea edulis larvae in the transmission and transfer of Bonamia ostreae.
[So] Source:J Invertebr Pathol;136:7-9, 2016 May.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, the ability of oyster larvae, brooded in the pallial cavity of the parent oyster, to become infected in the pallial fluid, which is influenced by the brooding oyster and surrounding environment, was investigated. Larvae were collected over three summers from three areas around Ireland. Samples were screened for the presence of Bonamia ostreae DNA using PCR analysis. Four samples of larvae were found to be positive for B. ostreae DNA, though the parent oysters were negative for infection. Larvae may be able to acquire the pathogen from the water column during filter feeding or elimination of pseudo-faeces by the brooding adult.
[Mh] Termos MeSH primário: Larva/microbiologia
Ostrea/microbiologia
Infecções por Protozoários/transmissão
[Mh] Termos MeSH secundário: Animais
Haplosporídios
Interações Hospedeiro-Parasita/fisiologia
Irlanda
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160217
[St] Status:MEDLINE


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[PMID]:26865235
[Au] Autor:Lane HS; Webb SC; Duncan J
[Ad] Endereço:Department of Zoology, University of Otago, PO Box 56, Dunedin 9054, New Zealand.
[Ti] Título:Bonamia ostreae in the New Zealand oyster Ostrea chilensis: a new host and geographic record for this haplosporidian parasite.
[So] Source:Dis Aquat Organ;118(1):55-63, 2016 Feb 11.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Previous reports of the haplosporidian parasite Bonamia ostreae have been restricted to the Northern Hemisphere, including Europe, and both eastern and western North America. This species is reported for the first time in New Zealand infecting the flat oyster Ostrea chilensis. Histological examination of 149 adult oysters identified 119 (79.9%) infected with Bonamia microcells. Bonamia generic PCR of several oysters followed by DNA sequencing of a 300 bp portion of the 18S rDNA gene produced a 100% match with that of B. ostreae. All DNA-sequenced products also produced a B. ostreae PCR-restriction fragment length polymorphism (PCR-RFLP) profile. Bonamia species-specific PCRs further detected single infections of B. exitiosa (2.7%), B. ostreae (40.3%), and concurrent infections (53.7%) with these 2 Bonamia species identifying overall a Bonamia prevalence of 96.6%. Detailed histological inspection revealed 2 microcell types. An infection identified by PCR as B. ostreae histologically presented small microcells (mean ± SE diameter = 1.28 ± 0.16 µm, range = 0.9-2 µm, n = 60) commonly with eccentric nuclei. A B. exitiosa infection exhibited larger microcells (mean ± SE diameter = 2.12 ± 0.27 µm, range = 1.5-4 µm, n = 60) with more concentric nuclei. Concurrent infections of both Bonamia species, as identified by PCR, exhibited both types of microcells. DNA barcoding of the B. ostreae-infected oyster host confirmed the identification as O. chilensis. A suite of other parasites that accompany O. chilensis are reported here for the first time in mixed infection with B. ostreae including apicomplexan X (76.5%), Microsporidium rapuae (0.7%) and Bucephalus longicornutus (30.2%).
[Mh] Termos MeSH primário: Haplosporídios/fisiologia
Ostrea/parasitologia
[Mh] Termos MeSH secundário: Animais
Interações Hospedeiro-Parasita
Nova Zelândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160211
[Lr] Data última revisão:
160211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160212
[St] Status:MEDLINE
[do] DOI:10.3354/dao02960


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[PMID]:26096446
[Au] Autor:Batista FM; López-Sanmartín M; Grade A; Navas JI; Ruano F
[Ad] Endereço:Divisão de Aquicultura e Valorização, Instituto Português do Mar e da Atmosfera (IPMA), Lisboa, Portugal.
[Ti] Título:Detection of Bonamia exitiosa in the European flat oyster Ostrea edulis in southern Portugal.
[So] Source:J Fish Dis;39(5):607-11, 2016 May.
[Is] ISSN:1365-2761
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Haplosporídios/fisiologia
Ostrea/parasitologia
[Mh] Termos MeSH secundário: Animais
Aquicultura
Haplosporídios/genética
Reação em Cadeia da Polimerase
Portugal
Proteínas de Protozoários/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Protozoan Proteins)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150623
[St] Status:MEDLINE
[do] DOI:10.1111/jfd.12396


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[PMID]:24779597
[Au] Autor:Yu H; Kong L; Li Q
[Ad] Endereço:a Key Laboratory of Mariculture , Ministry of Education, Ocean University of China , Qingdao , China.
[Ti] Título:Complete mitochondrial genome of Ostrea denselamellosa (Bivalvia, Ostreidae).
[So] Source:Mitochondrial DNA A DNA Mapp Seq Anal;27(1):711-2, 2016.
[Is] ISSN:2470-1408
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The complete mitochondrial (mt) genome of the flat oyster, Ostrea denselamellosa, was determined using Long-PCR and genome walking techniques in this study. The total length of the mt genome sequence of O. denselamellosa was 16,227 bp, which is the smallest reported Ostreidae mt genome to date. It contained 12 protein-coding genes (lacking of ATP8), 23 transfer RNA genes, and two ribosomal RNA genes. A bias towards a higher representation of nucleotides A and T (60.7%) was detected in the mt genome of O. denselamellosa. The rrnL was split into two fragments (3' half, 711 bp; 5' half, 509 bp), which seems to be the unique characteristics of Ostreidae mt genomes.
[Mh] Termos MeSH primário: Genoma Mitocondrial
Ostrea/genética
[Mh] Termos MeSH secundário: Animais
Composição de Bases/genética
Pareamento de Bases/genética
Sequência de Bases
DNA Mitocondrial/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Mitochondrial)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151215
[Lr] Data última revisão:
151215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140501
[St] Status:MEDLINE
[do] DOI:10.3109/19401736.2014.913154


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[PMID]:26341181
[Au] Autor:Vera M; Bello X; Álvarez-Dios JA; Pardo BG; Sánchez L; Carlsson J; Carlsson JE; Bartolomé C; Maside X; Martinez P
[Ad] Endereço:Departamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, Campus de Lugo, 27002 Lugo, Spain; Laboratori d'Ictiologia Genètica, Department de Biologia, Facultat de Ciències, Universitat de Girona, Campus Montilivi, 17071 Girona, Spain. Electronic address: manuel.vera@u
[Ti] Título:Screening of repetitive motifs inside the genome of the flat oyster (Ostrea edulis): Transposable elements and short tandem repeats.
[So] Source:Mar Genomics;24 Pt 3:335-41, 2015 Dec.
[Is] ISSN:1876-7478
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The flat oyster (Ostrea edulis) is one of the most appreciated molluscs in Europe, but its production has been greatly reduced by the parasite Bonamia ostreae. Here, new generation genomic resources were used to analyse the repetitive fraction of the oyster genome, with the aim of developing molecular markers to face this main oyster production challenge. The resulting oyster database, consists of two sets of 10,318 and 7159 unique contigs (4.8 Mbp and 6.8 Mbp in total length) representing the oyster's genome (WG) and haemocyte transcriptome (HT), respectively. A total of 1083 sequences were identified as TE-derived, which corresponded to 4.0% of WG and 1.1% of HT. They were clustered into 142 homology groups, most of which were assigned to the Penelope order of retrotransposons, and to the Helitron and TIR DNA-transposons. Simple repeats and rRNA pseudogenes, also made a significant contribution to the oyster's genome (0.5% and 0.3% of WG and HT, respectively).The most frequent short tandem repeats identified in WG were tetranucleotide motifs while trinucleotide motifs were in HT. Forty identified microsatellite loci, 20 from each database, were selected for technical validation. Success was much lower among WG than HT microsatellites (15% vs 55%), which could reflect higher variation in anonymous regions interfering with primer annealing. All microsatellites developed adjusted to Hardy-Weinberg proportions and represent a useful tool to support future breeding programmes and to manage genetic resources of natural flat oyster beds.
[Mh] Termos MeSH primário: Elementos de DNA Transponíveis/genética
Genoma
Repetições de Microssatélites/genética
Ostrea/genética
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Transposable Elements)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151215
[Lr] Data última revisão:
151215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150906
[St] Status:MEDLINE



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