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Pesquisa : B01.050.500.644.080.660 [Categoria DeCS]
Referências encontradas : 590 [refinar]
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[PMID]:29311448
[Au] Autor:Yamamoto A; Kudo S; Nakaya M; Hanaishi R; Masuda Y; Kimura J; Sakuraba A; Shibata M; Kudo S; Itsukaichi T; Sato H; Murakami A; Kogawa A
[Ad] Endereço:Aomori Prefectural Public Health and Environment Center.
[Ti] Título:[Validation Study of Analytical Method for Determination of Amnesic Shellfish Poison in Bivalves].
[So] Source:Shokuhin Eiseigaku Zasshi;58(6):281-287, 2017.
[Is] ISSN:1882-1006
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:Amnesic shellfish poison (ASP) is regarded as one of the shellfish poison groups in the EU, though it is not subject to regulation in Japan. We have developed an analytical method of ASP based on the report by Hatfield et al. and other methods. Validation studies were carried out with certified compositional reference materials (CRM). Performance parameters were estimated based on 17 analytical results. The estimate of trueness was 97.5%, and the estimate of intralaboratory reproducibility (RSD) was 1.5%. The HorRat(r) value was 0.16. These performance parameters meet the criteria in the Codex Procedural Manual. Furthermore, internal quality control was performed by using the CRM. The action limits were set based on the performance parameters of the method. Most of the results of the internal quality control were within the action limit range. The results confirmed that the quality of the analyses was well maintained. The purpose of the analytical method is to confirm that the level of ASP in scallop is less than 4.6 mg/kg. The applicability of the analytical method to scallops was confirmed by using spiked samples.
[Mh] Termos MeSH primário: Bivalves/química
Cromatografia Líquida de Alta Pressão/métodos
Ácido Caínico/análogos & derivados
Intoxicação por Frutos do Mar/etiologia
Intoxicação por Frutos do Mar/prevenção & controle
Frutos do Mar/análise
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão/instrumentação
Monitoramento Ambiental/métodos
Ácido Caínico/análise
Ácido Caínico/química
Ácido Caínico/toxicidade
Pectinidae/química
Controle de Qualidade
Reprodutibilidade dos Testes
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
M02525818H (domoic acid); SIV03811UC (Kainic Acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.3358/shokueishi.58.281


  2 / 590 MEDLINE  
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[PMID]:29182502
[Au] Autor:Kingston ACN; Chappell DR; Miller HV; Lee SJ; Speiser DI
[Ti] Título:Expression of G Proteins in the Eyes and Parietovisceral Ganglion of the Bay Scallop Argopecten irradians.
[So] Source:Biol Bull;233(1):83-95, 2017 Aug.
[Is] ISSN:1939-8697
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A multitude of image-forming eyes are spread across the bodies of certain invertebrates. Recent efforts have characterized how these eyes function, but less progress has been made toward describing the neural structures associated with them. Scallops, for example, have a distributed visual system that includes dozens of eyes whose optic nerves project to the lateral lobes of the parietovisceral ganglion (PVG). To identify sensory receptors and chemical synapses associated with the scallop visual system, we studied the expression of four G protein α subunits (Gα , Gα , Gα , and Gα ) in the eyes and PVG of the bay scallop Argopecten irradians (Lamarck, 1819). In the eyes of A. irradians, we noted expression of Gα by the ciliary photoreceptors of the distal retina, expression of Gα by the rhabdomeric photoreceptors of the proximal retina, and the expression of Gα and Gα by the cells of the cornea; we did not, however, detect expression of Gα or Gα in the eyes. In the PVG of A. irradians, we noted widespread expression of Gα , Gα , and Gα . The expression of Gα was limited to fine neurites in the lateral and ventral central lobes, as well as large unipolar neurons in the dorsal central lobes. Our findings suggest that light detection by the eyes of A. irradians is conferred primarily by photoreceptors that express Gα or Gα , that the corneal cells of scallops may contain sensory receptors and/or receive neural input, and that G protein labeling is useful for visualizing substructures and identifying specific populations of cells within the nervous systems of invertebrates.
[Mh] Termos MeSH primário: Subunidades alfa de Proteínas de Ligação ao GTP/genética
Expressão Gênica
Pectinidae/genética
[Mh] Termos MeSH secundário: Animais
Subunidades alfa de Proteínas de Ligação ao GTP/metabolismo
Gânglios dos Invertebrados/fisiologia
Perfilação da Expressão Gênica
Pectinidae/citologia
Células Fotorreceptoras de Invertebrados/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (GTP-Binding Protein alpha Subunits)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1086/694448


  3 / 590 MEDLINE  
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[PMID]:29182499
[Au] Autor:Faggionato D; Serb JM
[Ti] Título:Strategy to Identify and Test Putative Light-Sensitive Non-Opsin G-Protein-Coupled Receptors: A Case Study.
[So] Source:Biol Bull;233(1):70-82, 2017 Aug.
[Is] ISSN:1939-8697
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The rise of high-throughput RNA sequencing (RNA-seq) and de novo transcriptome assembly has had a transformative impact on how we identify and study genes in the phototransduction cascade of non-model organisms. But the advantage provided by the nearly automated annotation of RNA-seq transcriptomes may at the same time hinder the possibility for gene discovery and the discovery of new gene functions. For example, standard functional annotation based on domain homology to known protein families can only confirm group membership, not identify the emergence of new biochemical function. In this study, we show the importance of developing a strategy that circumvents the limitations of semiautomated annotation and apply this workflow to photosensitivity as a means to discover non-opsin photoreceptors. We hypothesize that non-opsin G-protein-coupled receptor (GPCR) proteins may have chromophore-binding lysines in locations that differ from opsin. Here, we provide the first case study describing non-opsin light-sensitive GPCRs based on tissue-specific RNA-seq data of the common bay scallop Argopecten irradians (Lamarck, 1819). Using a combination of sequence analysis and three-dimensional protein modeling, we identified two candidate proteins. We tested their photochemical properties and provide evidence showing that these two proteins incorporate 11-cis and/or all-trans retinal and react to light photochemically. Based on this case study, we demonstrate that there is potential for the discovery of new light-sensitive GPCRs, and we have developed a workflow that starts from RNA-seq assemblies to the discovery of new non-opsin, GPCR-based photopigments.
[Mh] Termos MeSH primário: Pectinidae/fisiologia
Receptores Acoplados a Proteínas-G/genética
Receptores Acoplados a Proteínas-G/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequenciamento de Nucleotídeos em Larga Escala
Luz
Transdução de Sinal Luminoso/genética
Pectinidae/genética
Pectinidae/metabolismo
Receptores Acoplados a Proteínas-G/química
Alinhamento de Sequência
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, G-Protein-Coupled)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1086/694842


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[PMID]:28939196
[Au] Autor:Zhang H; Shi Y; He M
[Ad] Endereço:CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing 100049, China.
[Ti] Título:Molecular identification of an insulin growth factor binding protein (IGFBP) and its potential role in an insulin-like peptide system of the pearl oyster, Pinctada fucata.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;214:27-35, 2017 Dec.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Insulin-like growth factors (IGFs) play critical roles in regulating metabolism, growth, and reproduction in invertebrates. IGF binding proteins (IGFBPs) serve as major regulators of IGF activity and regulate endocrine system. In the present study, the full-length cDNA of an igfbp was identified from the pearl oyster, Pinctada fucata, using expressed sequence tag (EST) sequence. The 1124bp Pfigfbp cDNA contains a 465bp open reading frame (ORF) encoding a putative protein of 154 amino acids, a 5'-untranslated region (UTR) of 238bp, and a 3'-UTR of 394bp (not including polyA+). Multiple sequence alignment of the deduced IB domain sequences revealed that twelve conserved Cys and ILP binding site in PfIGFBP were well aligned with human IGFBPs1-7, Mizuhopecten yessoensis IGFBP5 and Eriocheir sinensis IGFBP7. Gene expression analysis indicated that Pfigfbp mRNA was expressed in all the tissues and developmental stages examined, with a higher level in the foot than in other tissues and a higher level in the polar body stage and 32-cell stage than in the other stages. Pfigfbp and PfILP (insulin-like peptide) mRNA levels significantly increased in the digestive gland after feeding, while levels were dramatically reduced during a week of food deprivation and increased upon refeeding. In vitro experiments indicated that Pfigfbp mRNA expression in mantle cells was affected by insulin/IGFs (IGF-I, IGF-II). Our data suggests that Pfigfbp may be involved in endocrine signaling in P. fucata via the regulation of insulin-like peptide signaling.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica no Desenvolvimento
Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina/genética
Insulina/genética
Fases de Leitura Aberta
Pinctada/genética
Somatomedinas/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sítios de Ligação
Clonagem Molecular
Ingestão de Alimentos/genética
Escherichia coli/genética
Escherichia coli/metabolismo
Etiquetas de Sequências Expressas/química
Seres Humanos
Insulina/metabolismo
Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina/metabolismo
Especificidade de Órgãos
Pectinidae
Filogenia
Pinctada/classificação
Pinctada/crescimento & desenvolvimento
Pinctada/metabolismo
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Transdução de Sinais
Somatomedinas/metabolismo
Inanição/genética
Inanição/metabolismo
Regiões não Traduzidas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insulin); 0 (Insulin-Like Growth Factor Binding Proteins); 0 (Recombinant Proteins); 0 (Somatomedins); 0 (Untranslated Regions)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170924
[St] Status:MEDLINE


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[PMID]:28792420
[Au] Autor:Ji A; Li X; Fang S; Qin Z; Bai C; Wang C; Zhang Z
[Ad] Endereço:Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, PR China.
[Ti] Título:Primary culture of Zhikong scallop Chlamys farreri hemocytes as an in vitro model for studying host-pathogen interactions.
[So] Source:Dis Aquat Organ;125(3):217-226, 2017 08 09.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Primary cultured cells can be a useful tool in studies on physiology, virology, and toxicology. Hemocytes play an important role in animal rapid response to pathogen invasion. In this study, an appropriate medium for primary culture of hemocytes of the bivalve Chlamys farreri was developed by adding 5% fetal bovine serum and 1% C. farreri serum to Leibovitz L-15 medium. These primary cultured hemocytes were maintained for more than 40 d in vitro and were classified into 3 types: (1) granulocytes containing numerous granules in the cytoplasm, (2) hyalinocytes with no or few granules, (3) a small percentage of macrophage-like cells. Furthermore, the primary cultured hemocytes were observed to be sensitive to bacterial and viral challenges. These hemocytes could phagocytose the bacterium Vibrio anguillarum, and presented cytopathic effects on the extracellular products (ECPs) of V. anguillarum; the mRNA level of QM, which plays an important role in immune response, also significantly increased 12 h after infection. When these hemocytes were challenged with ostreid herpesvirus 1 (OsHV-1), virus particles and empty capsids in the cells infected for 48 h were observed by transmission electron microscopy, and the QM mRNA level increased significantly at 12 h and 24 h following OsHV-1 challenge. This primary culture system is available for C. farreri hemocytes which can be used in the future to study host-pathogen interactions.
[Mh] Termos MeSH primário: Hemócitos/fisiologia
Herpesviridae/fisiologia
Pectinidae/citologia
Vibrio/fisiologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Cultura de Células
Regulação da Expressão Gênica/imunologia
Hemócitos/microbiologia
Interações Hospedeiro-Patógeno
RNA Viral
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.3354/dao03145


  6 / 590 MEDLINE  
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[PMID]:28723591
[Au] Autor:Fan S; Xu Y; Liu B; He W; Zhang B; Su J; Yu D
[Ad] Endereço:Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, PR China.
[Ti] Título:Molecular characterization and expression analysis of the myostatin gene and its association with growth traits in Noble scallop (Chlamys nobilis).
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;212:24-31, 2017 Oct.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Myostatin (MSTN), also called growth and differentiation factor-8 (GDF-8), is a member of the transforming growth factor-ß (TGF-ß) superfamily and an inhibitor of muscle differentiation and growth. In this report, we identified and characterized a MSTN gene (CnMSTN) from the scallop Chlamys nobilis. The open reading frame of CnMSTN was 1374bp in length, encoding 457 amino acids. The structure of CnMSTN included a putative signal peptide, a TGF-ß propeptide domain, and a conserved TGF-ß domain. Phylogenetic analysis showed that the CnMSTN gene was clustered in the same subgroup with the MSTN gene found in Mollusca. Quantitative real-time PCR showed that the CnMSTN gene was widely expressed in all tissues tested, with the highest expression level observed in the adductor muscle. Six single nucleotide polymorphisms (SNPs) were identified in the promoter region, but no SNP was detected in the exon regions. Association analysis showed that SNP g.-579A/C had significant effects on body mass, soft-tissue mass, and adductor muscle mass. The CC and AC genotypes of g.-579A/C had significantly higher growth trait values than that of genotype AA (P<0.05). These results suggest that CnMSTN could be used as a candidate gene for the selective breeding of C. nobilis.
[Mh] Termos MeSH primário: Miostatina/genética
Pectinidae/crescimento & desenvolvimento
Pectinidae/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Clonagem Molecular
DNA Complementar/genética
Regulação da Expressão Gênica no Desenvolvimento
Estudos de Associação Genética
Miostatina/química
Pectinidae/metabolismo
Filogenia
Polimorfismo de Nucleotídeo Único
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Homologia de Sequência de Aminoácidos
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Myostatin); 0 (RNA, Messenger)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE


  7 / 590 MEDLINE  
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[PMID]:28665994
[Au] Autor:Yang D; Zhang Z; Liang S; Yang Q; Wang Y; Qin Z
[Ad] Endereço:Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao, China.
[Ti] Título:A novel role of Krüppel-like factor 4 in Zhikong scallop Chlamys farreri during spermatogenesis.
[So] Source:PLoS One;12(6):e0180351, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Krüppel-like factor 4 (KLF4) is a kind of zinc finger transcription factor, which is involved in terminal differentiation of epithelial cells and reprogramming of somatic cells to induced pluripotent stem (iPS) cells in mammals. In the present study, we identified a full-length cDNA of Klf4 in Zhikong scallop Chamys farreri (Cf-Klf4) and found that Cf-Klf4 presented a sexual dimorphic expression characteristic in C. farreri gonads. Cf-Klf4 expression was significantly higher in testes than in ovaries from growing stage to mature stage detected by quantitative real-time PCR, and was located in male gametes, except for spermatozoa during spermatogenesis through in situ hybridization and immunohistochemistry, while no positive signal was visible in female gametes during oogenesis. Furthermore, the knockdown of Cf-Klf4 in testes by means of in vivo RNA interference led to an obviously developmental retardance, lower gonadosomatic index, less male gametes and more apoptotic spermatocytes. Interestingly, we found that two out of eight scallops showed a hermaphroditic phenotype characteristic of male-to-female sex reversal when the Klf4 mRNA and protein levels were knocked down in males. These results verified that Klf4 plays an important role in testis functional maintenance and is necessary in spermatogenesis of C. farreri.
[Mh] Termos MeSH primário: Fatores de Transcrição Kruppel-Like/fisiologia
Espermatogênese/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
DNA Complementar
Fatores de Transcrição Kruppel-Like/química
Fatores de Transcrição Kruppel-Like/genética
Masculino
Pectinidae
Interferência de RNA
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (GKLF protein); 0 (Kruppel-Like Transcription Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180351


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[PMID]:28627495
[Au] Autor:Meyer GR; Lowe GJ; Gilmore SR; Bower SM
[Ad] Endereço:Fisheries and Oceans Canada, Pacific Biological Station, Nanaimo, BC V9T 6N7, Canada.
[Ti] Título:Disease and mortality among Yesso scallops Patinopecten yessoensis putatively caused by infection with Francisella halioticida.
[So] Source:Dis Aquat Organ;125(1):79-84, 2017 Jun 19.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:During the fall of 2015, up to 40% mortality occurred in juvenile Yesso scallops Patinopecten yessoensis at an aquaculture site in Baynes Sound, British Columbia, Canada. Macroscopic lesions were present in 11% of the scallops, and histopathology consisting of multifocal and diffuse haemocyte infiltration was observed in 44% of the specimens examined. Histologically, small Gram-negative intracellular bacteria-like particles were observed within necrotic haemocytes of the lesions, suggesting a bacterial aetiology. DNA was extracted from adductor muscle lesions of diseased scallops, and the 16s rDNA gene as well as the DNA-directed RNA polymerase beta subunit (rpoB) were amplified by PCR. Sequence analyses of the resulting 413 and 925 bp fragments were a 100% match to the reference sequence for Francisella halioticida, originally described as the cause of mortality in abalone from Japan. Isolation and culture of the bacteria was not possible at the time, as no further diseased specimens were available. Results from in situ hybridization assays as well as examination by transmission electron microscopy provide further evidence supporting the hypothesis that F. halioticida was the most probable causative agent of the lesions and mortality.
[Mh] Termos MeSH primário: Francisella/fisiologia
Pectinidae/microbiologia
[Mh] Termos MeSH secundário: Animais
Interações Hospedeiro-Patógeno
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.3354/dao03130


  9 / 590 MEDLINE  
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[PMID]:28397607
[Au] Autor:Otani A; Nakajima T; Okumura T; Fujii S; Tomooka Y
[Ad] Endereço:1 Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.
[Ti] Título:Sex Reversal and Analyses of Possible Involvement of Sex Steroids in Scallop Gonadal Development in Newly Established Organ-Culture Systems.
[So] Source:Zoolog Sci;34(2):86-92, 2017 Apr.
[Is] ISSN:0289-0003
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Many molluscs perform sex reversal, and sex hormones may be involved in the process. In adult scallops, Patinopecten yessoensis, gonadotropin releasing hormone and 17ß-estradiol (E ) are involved in male sexual maturation, however, little is known about the effects of E and testosterone (T) on the gonadal differentiation in young scallops. In the present study, scallop gonadal development was analyzed to determine the sex reversal stage in Funka bay, and effects of E and T were examined. In Funka bay, almost all scallops were male at month 12. Scallops equipped with ambiguous gonads were 61.1% at month 16 and disappeared at month 18. Therefore, sex reversal in Funka bay occurs at around month 16. For establishment of organ culture systems for bivalves, Manila clam gonads were cultured in 15% L-15 medium diluted with HBSS containing 10% KSR on agarose gel at 10°C, and the gonads survived for 14 days. Scallop gonads were also able to be cultured in 30% L15 medium diluted with ASW containing 10% KSR on agarose gel for seven days. At mature stage, Foxl2 and Tesk were predominantly expressed in ovary and testis, respectively. When scallop gonads at sex reversal stage were organ-cultured, sex steroid treatment decreased Tesk expression in the majority of scallop gonads at sex reversal stage. However, no obvious change in Foxl2 and Tesk expression was detected in mature gonads in response to either E or T in culture, suggesting sex steroid treatment might affect gonadal development at sex reversal stage.
[Mh] Termos MeSH primário: Estradiol/farmacologia
Gônadas/efeitos dos fármacos
Gônadas/fisiologia
Técnicas de Cultura de Órgãos/métodos
Pectinidae/fisiologia
Testosterona/farmacologia
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Diferenciação Sexual/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3XMK78S47O (Testosterone); 4TI98Z838E (Estradiol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.2108/zs160070


  10 / 590 MEDLINE  
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[PMID]:28325219
[Au] Autor:Mazón-Suástegui JM; García-Bernal M; Saucedo PE; Campa-Córdova Á; Abasolo-Pacheco F
[Ad] Endereço:Centro de Investigaciones Biológicas del Noroeste (CIBNOR), La Paz, B.C.S. 23096, Mexico.
[Ti] Título:Homeopathy outperforms antibiotics treatment in juvenile scallop Argopecten ventricosus: effects on growth, survival, and immune response.
[So] Source:Homeopathy;106(1):18-26, 2017 Feb.
[Is] ISSN:1476-4245
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mortality from vibriosis in mollusk production is attributed to pathogenic bacteria, particularly Vibrio alginolyticus. Use of increasingly potent antibiotics has led to bacterial resistance and increased pathogenicity. Alternatives in sanitation, safety, and environmental sustainability are currently under analysis. To-date, homeopathy has been investigated in aquaculture of freshwater fish, but not in marine mollusks. The effect of the homeopathic complexes in the growth, survival, and immune response of the Catarina scallop Argopecten ventricosus were assessed. METHODS: A bioassay to assess the potential of homeopathy in improving cultivation of juvenile A. ventricosus was conducted for 21 days, with a final challenge of 120 h with V. alginolyticus. The experimental design included two homeopathic formulas The homeopathic complex Passival, consisting of Passiflora incarnata 30 CH, Valeriana officinalis 30 CH, Ignatia amara 30 CH and Zincum valerianicum 30 CH plus Phosphoricum acid 30 CH (treatment TH1) or Silicea terra 30 CH (TH2), two antibiotics (ampicillin = AMP, oxytetracycline = OXY), and two reference treatments (without homeopathic or antibiotic treatment = CTRL, ethanol 30° GL = ETH). Additionally, a negative control CTRL- (untreated/uninfected) is included in the challenge test. Juvenile scallops (4.14 ± 0.06 mm, 13.33 mg wet weight) were cultivated in 4 L tanks provided with aerated, filtered (1 µm), and UV-sterilized seawater that was changed every third day. They were fed a blend of the microalgae Isochrysis galbana and Chaetoceros calcitrans (150,000 cells mL twice a day). All treatments were directly added to the tank water and then 500 mL challenge units were inoculated with 1 × 10 CFU/mL (LD ) of V. alginolyticus. RESULTS: Juveniles grew significantly larger and faster in height and weight with TH2 compared to the ETH and CTRL (P < 0.05, ANOVA). Higher concentrations of proteins occurred in scallops exposed to TH2 (160.57 ± 7.79 mg g ), compared to other treatments and reference treatments. Higher survival rate during the challenge bioassay occurred with TH1 (85%), compared to AMP (53%), OXY (30%), and CTRL (0%), and superoxide dismutase (P < 0.05) was significantly higher in scallops treated with TH1, compared to other treatments and reference treatments. CONCLUSIONS: Homeopathic treatments improved growth and survival and enhanced survival against V. alginolyticus in juvenile A. ventricosus. This suggests that homeopathy is a viable treatment for this mollusk to reduce use of antibiotics in scallops and its progressive increase in pathogenicity in mollusk hatcheries.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Homeopatia
Pectinidae/microbiologia
Preparações de Plantas/farmacologia
Vibrio/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Interações Hospedeiro-Patógeno
Pectinidae/imunologia
Pectinidae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Plant Preparations)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE



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