Base de dados : MEDLINE Pesquisa : B01.050.500.644.080.850.050 [Categoria DeCS] Referências encontradas : 60 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 6

ir para página

1 / 60

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 28336488 [Au] Autor: Liu Q; Shang X; Ma Y; Xia X; Xue S; Hua C; Liang G; Yao L; Guo L [Ad] Endereço: Basic Medicine Institution of Nanyang Medical College, Nanyang, 473041, Henan Province, China. [Ti] Título: Isolation and characterization of two glutathione S-transferases from freshwater bivalve Anodonta woodiana: Chronic effects of pentachlorophenol on gene expression profiles. [So] Source: Fish Shellfish Immunol;64:339-351, 2017 May. [Is] ISSN: 1095-9947 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Glutathione S-transferases (GST) play a prominent role in protecting cells against oxidative stress. Our previous study showed that the reactive oxygen species (ROS) generated from pentachlorophenol (PCP) could cause an acute impact on freshwater bivalve Anodonta Woodiana, but its chronic toxicity remain unclear. In order to investigate the chronic effect of PCP, clams A. Woodiana were randomly grouped into PCP treated group in which animals were administrated with 13.9 µg/L concentrations of PCP, and control group those with similar volume dimethyl sulfoxide. In addition, two complete GST sequences were isolated from A. Woodianaa and respectively named AwGST1 and AwGST2. The full-length cDNA of AwGST1 was consisted of a 5' untranslated region (UTR) of 132 bp, a 3' UTR of 80 bp and an open reading frame (ORF) of 609 bp encoding a polypeptide of 203 amino acids. The full-length cDNA of AwGST2 contained a 5' UTR of 57 bp, a 3' UTR of 291 bp and an ORF of 678 bp encoding a polypeptide of 226 amino acids. The constitutive expression levels of AwGST1 and AwGST2 were examined in different tissues including foot, mantle, adductor muscle, heart, hepatopancreas, hemocytes and gill. Administration of PCP could result in a significant increase of AwGST1 and AwGST2 expression in the hepatopancreas, gill and hemocytes. In the hepatopancreas, AwGST1 mRNA levels of PCP treated group increased more than 28.73% at day 1, then 70.37% (P < 0.05) at day 3, reach to 6.64 times (P < 0.01) at day 15 in contrasted with that of control group. AwGST2 increased more 18.18%, 82.88% (P < 0.05) and 2.43 times (P < 0.01) at day 1, 3 and 15, respectively. In the gill, AwGST1 expression showed a significant up-regulation in the PCP treated group during experiment observed compared with that of control group, mRNA level of AwGST2 increased more than 1.44 times (P < 0.05). In addition, expressions of AwGST1 and AwGST2 were significantly induced after PCP treatment in the hemocytes. These results indicated that up-regulations of AwGST1 and AwGST2 expression in bivalve A. woodiana are contribute to against oxidative stress derived from PCP treatment during experiment observed. [Mh] Termos MeSH primário: Anodonta/efeitos dos fármacos Anodonta/genética Glutationa Transferase/genética Pentaclorofenol/toxicidade Transcriptoma/efeitos dos fármacos Poluentes Químicos da Água/toxicidade [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Sequência de Bases Clonagem Molecular DNA Complementar/genética DNA Complementar/metabolismo Glutationa Transferase/química Glutationa Transferase/metabolismo Filogenia Estrutura Secundária de Proteína Estrutura Terciária de Proteína RNA Mensageiro/genética RNA Mensageiro/metabolismo Distribuição Aleatória Alinhamento de Sequência [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Complementary); 0 (RNA, Messenger); 0 (Water Pollutants, Chemical); D9BSU0SE4T (Pentachlorophenol); EC 2.5.1.18 (Glutathione Transferase) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170628 [Lr] Data última revisão: 170628 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170325 [St] Status: MEDLINE

2 / 60

MEDLINE

seleciona para imprimir Fotocópia

[PMID]: 27616202 [Au] Autor: Ismail NS; Tommerdahl JP; Boehm AB; Luthy RG [Ad] Endereço: Department of Civil and Environmental Engineering, Stanford University , Stanford, California 94305, United States. [Ti] Título: Escherichia coli Reduction by Bivalves in an Impaired River Impacted by Agricultural Land Use. [So] Source: Environ Sci Technol;50(20):11025-11033, 2016 Oct 18. [Is] ISSN: 1520-5851 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Fecal indicator bacteria (FIB) are leading causes of impaired surface waters. Innovative and environmentally appropriate best management practices are needed to reduce FIB concentrations and associated risk. This study examines the ability of the native freshwater mussel Anodonta californiensis and an invasive freshwater clam Corbicula fluminea to reduce concentrations of the FIB Escherichia coli in natural waters. Laboratory batch experiments were used to show bivalve species-specific E. coli removal capabilities and to develop a relationship between bivalve size and clearance rates. A field survey within an impaired coastal river containing both species of bivalves in an agricultural- and grazing-dominated area of the central coast of California showed a significant inverse correlation between E. coli concentration and bivalve density. An in situ field spiking and sampling study showed filtration by freshwater bivalves resulting in 1-1.5 log reduction of E. coli over 24 h, and calculated clearance rates ranged from 1.2 to 7.4 L hr bivalve . Results of this study show the importance of freshwater bivalves for improving water quality through the removal of E. coli. While both native and invasive bivalves can reduce E. coli levels, the use of native bivalves through integration into best management practices is recommended as a way to improve water quality and protect and encourage re-establishment of native bivalve species that are in decline. [Mh] Termos MeSH primário: Escherichia coli Rios [Mh] Termos MeSH secundário: Animais Anodonta Corbicula Água Doce [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170608 [Lr] Data última revisão: 170608 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160913 [St] Status: MEDLINE

3 / 60

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 27435976 [Au] Autor: Liu H; Chen X; Su Y; Kang IJ; Qiu X; Shimasaki Y; Oshima Y; Yang J [Ad] Endereço: Key Laboratory of Fishery Ecological Environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, People's Republic of China. [Ti] Título: Effects of Calcium and Magnesium Ions on Acute Copper Toxicity to Glochidia and Early Juveniles of the Chinese Pond Mussel Anodonta woodiana. [So] Source: Bull Environ Contam Toxicol;97(4):504-9, 2016 Oct. [Is] ISSN: 1432-0800 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: We evaluated the effects of calcium (Ca(2+)) and magnesium (Mg(2+)) ions on copper (Cu) toxicity to glochidia and newly-transformed juvenile mussels (age 1-2 days) of the Chinese pond mussel (Anodonta woodiana). Acute Cu toxicity tests were performed with glochidia for 24 h and juveniles for 96 h with measured Ca(2+) concentrations of 1.1, 14, 26, 51, and 99 mg L(-1), or measured Mg(2+) concentrations of 2.6, 11, 21, and 39 mg L(-1). The Ca(2+) and Mg(2+) cations provided no statistically significant protection against Cu toxicity to glochidia or juveniles. The 24-h EC50 value for glochidia was 82 µg L(-1) Cu, and contrastly, 96-h EC50 value for newly-transformed juvenile mussels was as low as 12 µg L(-1) Cu, implying that the juveniles of A. woodiana are more vulnerable to Cu contamination at concentrations close to currently-accepted levels. [Mh] Termos MeSH primário: Anodonta/efeitos dos fármacos Cálcio/química Cobre/toxicidade Magnésio/química [Mh] Termos MeSH secundário: Animais Água Doce Íons Tanques Testes de Toxicidade Aguda Poluentes Químicos da Água/toxicidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Ions); 0 (Water Pollutants, Chemical); 789U1901C5 (Copper); I38ZP9992A (Magnesium); SY7Q814VUP (Calcium) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170117 [Lr] Data última revisão: 170117 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160721 [St] Status: MEDLINE [do] DOI: 10.1007/s00128-016-1890-8

4 / 60

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 27291351 [Au] Autor: Xia X; Hua C; Xue S; Shi B; Gui G; Zhang D; Wang X; Guo L [Ad] Endereço: Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China; State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Centre for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: xiaxichao8336@163. [Ti] Título: Response of selenium-dependent glutathione peroxidase in the freshwater bivalve Anodonta woodiana exposed to 2,4-dichlorophenol,2,4,6-trichlorophenol and pentachlorophenol. [So] Source: Fish Shellfish Immunol;55:499-509, 2016 Aug. [Is] ISSN: 1095-9947 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP), and pentachlorophenol (PCP) pose a health risk to aquatic organism and humans, and are recognized as persistent priority pollutants. Selenium dependent glutathione peroxidase (Se-GPx) belongs to the family of selenoprotein, which acts mainly as an antioxidant role in the cellular defense system. In the current study, a Se-GPx full length cDNA was cloned from Anodonta woodiana and named as AwSeGPx. It had a characteristic codon at 165TGA167 that corresponds to selenocysteine(Sec) amino acid as U44. The full length cDNA consists of 870 bp, an open reading frame (ORF) of 585 bp encoded a polypeptide of 195 amino in which conserved domain (68LGFPCNQF75) and a glutathione peroxide-1 GPx active site (32GKVILVENVASLUGTT47) were observed. Additionally, the eukaryotic selenocysteine insertion sequence (SECIS) was conserved in the 3'UTR. The AwSeGPx amino acid sequence exhibited a high similarity with that of other Se-GPx. Real-time PCR analysis revealed that AwSeGPx mRNA had a widely distribution, but the highest level was observed in hepatopancreas. AwSeGPx mRNA expression was significantly up-regulated in hepatopancreas, gill and hemocytes after 2,4-DCP, 2,4,6-TCP and PCP exposure. Under similar environment, clams A. woodiana showed a more sensitive to PCP than that of 2,4-DCP and 2,4,6-TCP. These results indicate that AwSeGPx plays a protective role in eliminating oxidative stress derived from 2,4-DCP, 2,4,6-TCP and PCP treatment. [Mh] Termos MeSH primário: Anodonta/efeitos dos fármacos Anodonta/genética Glutationa Peroxidase/genética Poluentes Químicos da Água/toxicidade [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Anodonta/metabolismo Sequência de Bases Clorofenóis/toxicidade Clonagem Molecular DNA Complementar/genética DNA Complementar/metabolismo Glutationa Peroxidase/química Glutationa Peroxidase/metabolismo Pentaclorofenol/toxicidade Filogenia Conformação Proteica Estrutura Secundária de Proteína RNA Mensageiro/genética RNA Mensageiro/metabolismo Distribuição Aleatória Alinhamento de Sequência [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Chlorophenols); 0 (DNA, Complementary); 0 (RNA, Messenger); 0 (Water Pollutants, Chemical); D9BSU0SE4T (Pentachlorophenol); EC 1.11.1.9 (Glutathione Peroxidase); MHS8C5BAUZ (2,4,6-trichlorophenol); R669TG1950 (2,4-dichlorophenol) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170302 [Lr] Data última revisão: 170302 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160614 [St] Status: MEDLINE

5 / 60

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 27037470 [Au] Autor: Qu H; Ma RX; Liu DH; Jing X; Wang F; Zhou ZQ; Wang P [Ad] Endereço: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, China Agricultural University, Beijing 100193, PR China. [Ti] Título: The toxicity, bioaccumulation, elimination, conversion of the enantiomers of fipronil in Anodonta woodiana. [So] Source: J Hazard Mater;312:169-174, 2016 07 15. [Is] ISSN: 1873-3336 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: The enantioselective bioaccumulation and elimination of fipronil in Anodonta woodiana (A. woodiana) were studied and the main metabolites fipronil desulfinyl, fipronil sulfide and fipronil sulfone were determined. The acute toxicity of the enantiomers of fipronil and the three metabolites were also investigated. In the bioaccumulation process, fipronil in A. woodiana reached equilibrium after 11days with BCF value of 0.2, and the enantiomeric fraction (EF) values showed that the bioaccumulation was enantioselective with enantioenrichment of S-fipronil. The degradation of fipronil in A. woodiana fitted first-order kinetics model with half-lives of the enantiomers were 5.8 d for R-fipronil and 7.6 d for S-fipronil, and the EF values decreasing from 0.5 gradually indicating the R-enantiomer was preferentially degraded. The degradation of single enantiomers was also performed and the results revealed a fast conversion of R-fipronil to S-fipronil by A. woodiana. The three metabolites were all detected in A. woodiana-water system, in which fipronil sulfone and fipronil sulfide had higher concentration levels. According to the 72-h LC50 values, S-fipronil was much more toxic than the racemate and R-fipronil. Moreover, the metabolites were more toxic than the parent fipronil. The results suggested the individual enantiomers of chiral pollutants and the metabolites should be considered in the risk assessments. [Mh] Termos MeSH primário: Anodonta/metabolismo Inseticidas/metabolismo Pirazóis/metabolismo Poluentes Químicos da Água/metabolismo [Mh] Termos MeSH secundário: Animais Cinética Estereoisomerismo Sulfetos/metabolismo Sulfonas/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Insecticides); 0 (Pyrazoles); 0 (Sulfides); 0 (Sulfones); 0 (Water Pollutants, Chemical); QGH063955F (fipronil) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171017 [Lr] Data última revisão: 171017 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160403 [St] Status: MEDLINE

6 / 60

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 26915310 [Au] Autor: Xia X; Huang C; Zhang D; Zhang Y; Xue S; Wang X; Zhang Q; Guo L [Ad] Endereço: Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China; State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Centre for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: xiaxichao8336@163. [Ti] Título: Molecular cloning, characterization, and the response of Cu/Zn superoxide dismutase and catalase to PBDE-47 and -209 from the freshwater bivalve Anodonta woodiana. [So] Source: Fish Shellfish Immunol;51:200-210, 2016 Apr. [Is] ISSN: 1095-9947 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Polybrominated diphenyl ethers-47 (PBDE-47) and -209 are significant components of total PBDEs in water and can catalyze the production of reactive oxygen species (ROS) in the organisms. Anti-oxidant enzymes play an important role in scavenging the high level of ROS. In the current study, two full-length cDNAs of Cu/Zn superoxide dismutase (CuZnSODs) and catalase (CAT) were isolated from freshwater bivalve Anodonta woodiana by rapid amplification of cDNA ends approach and respectively named as AwSOD and AwCAT. The nucleotide sequence of AwSOD cDNA had an open reading frame (ORF) of 465 bp encoding a polypeptide of 155 amino acids in which signature 1 GKHGFHVHEFGDNT and signature 2 GNAGARSACGVI of SODs were observed. Deduced amino acid sequence of AwSOD showed a significant similarity with that of CuZnSODs. AwCAT had an ORF 1536 bp encoding a polypeptide of 512 amino acids which contains a conserved catalytic site motif, and a proximal heme-ligand signature motif of CATs. The time-course expressions of AwSOD and AwCAT in hepatopancreas were measured by quantitative real-time PCR. Expressions of AwSOD and AwCAT showed a significant up-regulation in groups at a low concentration treatment of PBDE-47, a biphasic pattern in groups with a high concentration treatment. Administration of PBDE-209 could result in an up-regulation of AwSOD and AwCAT expressions with time- and dose-dependent matter. These results indicate that up-regulations of AwSOD and AwCAT expression of hepatopancreas of freshwater bivalve A. woodiana contribute to eliminate oxidative stress derived from PBDE-47 and -209 treated. [Mh] Termos MeSH primário: Anodonta/efeitos dos fármacos Catalase/genética Éteres Difenil Halogenados/toxicidade Superóxido Dismutase-1/genética [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Anodonta/genética Sequência de Bases Clonagem Molecular DNA Complementar/genética Água Doce Expressão Gênica/efeitos dos fármacos Hepatopâncreas/efeitos dos fármacos Hepatopâncreas/metabolismo Regulação para Cima [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (DNA, Complementary); 0 (Halogenated Diphenyl Ethers); 0N97R5X10X (2,2',4,4'-tetrabromodiphenyl ether); EC 1.11.1.6 (Catalase); EC 1.15.1.1 (Superoxide Dismutase-1); N80BQ29A0H (decabromobiphenyl ether) [Em] Mês de entrada: 1612 [Cu] Atualização por classe: 170728 [Lr] Data última revisão: 170728 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160227 [St] Status: MEDLINE

7 / 60

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 26599145 [Au] Autor: Bielen A; Bosnjak I; Sepcic K; Jaklic M; Cvitanic M; Lusic J; Lajtner J; Simcic T; Hudina S [Ad] Endereço: University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Pierottijeva 6, 10000 Zagreb, Croatia. Electronic address: abielen@pbf.hr. [Ti] Título: Differences in tolerance to anthropogenic stress between invasive and native bivalves. [So] Source: Sci Total Environ;543(Pt A):449-59, 2016 Feb 01. [Is] ISSN: 1879-1026 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Tolerance towards environmental stress has been frequently considered as one of the key determinants of invasion success. However, empirical evidence supporting the assumption that invasive species can better endure unfavorable conditions compared with native species is limited and has yielded opposing results. In this study, we examined the tolerance to different stress conditions (thermal stress and trace metal zinc pollution stress) in two phylogenetically related and functionally similar freshwater bivalve species, the native Anodonta anatina and the invasive Sinanodonta woodiana. We assessed potential differences in response to stress conditions using several cellular response assays: efficiency of the multixenobiotic resistance mechanism, respiration estimate (INT reduction capacity), and enzymatic biomarkers. Our results demonstrated that the invasive species overall coped much better with unfavorable conditions. The higher tolerance of S. woodiana was evident from (i) significantly decreased Rhodamine B accumulation indicating more efficient multixenobiotic resistance mechanism; (ii) significantly higher INT reduction capacity and (iii) less pronounced alterations in the activity of stress-related enzymes (glutathione-S-transferase, catalase) and of a neurotoxicity biomarker (cholinesterase) in the majority of treatment conditions in both stress trials. Higher tolerance to thermal extremes may provide physiological benefit for further invasion success of S. woodiana in European freshwaters, especially in the context of climate change. [Mh] Termos MeSH primário: Mudança Climática Monitoramento Ambiental Estresse Fisiológico Poluentes Químicos da Água/análise [Mh] Termos MeSH secundário: Animais Anodonta/fisiologia Biomarcadores/metabolismo Catalase/metabolismo Colinesterases/metabolismo Água Doce/química Glutationa Transferase/metabolismo Espécies Introduzidas Estresse Oxidativo Unionidae/fisiologia Poluentes Químicos da Água/toxicidade [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Biomarkers); 0 (Water Pollutants, Chemical); EC 1.11.1.6 (Catalase); EC 2.5.1.18 (Glutathione Transferase); EC 3.1.1.8 (Cholinesterases) [Em] Mês de entrada: 1609 [Cu] Atualização por classe: 151222 [Lr] Data última revisão: 151222 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151125 [St] Status: MEDLINE

8 / 60

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 26525828 [Au] Autor: Lopes-Lima M; Hinzmann M; Teixeira A; Varandas S; Machado J; Sousa R; Froufe E [Ad] Endereço: CIIMAR/CIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal. [Ti] Título: The strange case of the tetragenous Anodonta anatina. [So] Source: J Exp Zool A Ecol Genet Physiol;325(1):52-6, 2016 Jan. [Is] ISSN: 1932-5231 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Unionoid freshwater mussels have a unique life cycle with a form of parental care where the larvae are developed and kept inside the gills until release, followed by an obligate parasitic stage on fish. The size and location of the marsupium have been used as important phylogenetic characters in unionoids and in Anodontini its location was described exclusively on the outer demibranchs. Two recent surveys in a lake in the North of Portugal revealed large anodontine mussels morphological identical to Anodonta anatina but with glochidia in both demibranchs and with an unusual large size. In order to establish the identity of these mussels, a barcoding approach was used and an anatomical description of the gills and glochidia was performed. These mussels were identified as A. anatina and presented an inner demibranch pair with tripartite tubes. The glochidial sizes were much higher than previously reported for the species reaching maximum (length × height) values of 566 × 552 µm. This species reveals a high ecological plasticity being able to change its size and anatomy to increase its fertility as well as infestation performance. J. Exp. Zool. 325A:52-56, 2016. © 2015 Wiley Periodicals, Inc. [Mh] Termos MeSH primário: Anodonta/crescimento & desenvolvimento Larva/crescimento & desenvolvimento Estágios do Ciclo de Vida Filogenia [Mh] Termos MeSH secundário: Animais Anodonta/genética Anodonta/patogenicidade Ecossistema Peixes/parasitologia Água Doce Brânquias/parasitologia Lagos Larva/classificação [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151104 [St] Status: MEDLINE [do] DOI: 10.1002/jez.1995

9 / 60

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 26187809 [Au] Autor: Hartmann JT; Beggel S; Auerswald K; Stoeckle BC; Geist J [Ad] Endereço: Aquatic Systems Biology Unit, Department of Ecology and Ecosystem Management, Technische Universitaet Muenchen, Muehlenweg 22, D-85350 Freising, Germany. [Ti] Título: Establishing mussel behavior as a biomarker in ecotoxicology. [So] Source: Aquat Toxicol;170:279-88, 2016 Jan. [Is] ISSN: 1879-1514 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Most freshwater mussel species of the Unionoida are endangered, presenting a conservation issue as they are keystone species providing essential services for aquatic ecosystems. As filter feeders with limited mobility, mussels are highly susceptible to water pollution. Despite their exposure risk, mussels are underrepresented in standard ecotoxicological methods. This study aimed to demonstrate that mussel behavioral response to a chemical stressor is a suitable biomarker for the advancement of ecotoxicology methods that aids mussel conservation. Modern software and Hall sensor technology enabled mussel filtration behavior to be monitored real-time at very high resolution. With this technology, we present our method using Anodonta anatina and record their response to de-icing salt pollution. The experiment involved an environmentally relevant 'pulse-exposure' design simulating three subsequent inflow events. Three sublethal endpoints were investigated, Filtration Activity, Transition Frequency (number of changes from opened to closed, or vice versa) and Avoidance Behavior. The mussels presented a high variation in filtration behavior, behaving asynchronously. At environmentally relevant de-icing salt exposure scenarios, A. anatina behavior patterns were significantly affected. Treated mussels' Filtration Activity decreased during periods of very high and long de-icing salt exposure (p<0.001), however, increased during short de-icing salt exposure. Treated mussels' Transition Frequency increased during periods of very high and long de-icing salt exposure (p<0.001), which mirrored the Avoidance Behavior endpoint observed only by mussels under chemical stress. Characteristics of Avoidance Behavior were tighter shell closures with repeated and irregular shell movements which was significantly different to their undisturbed resting behavior (p<0.001). Additionally, we found that mussels were sensitive to a chemical stressor even when the mussel's valves were closed. Due to the effects of de-icing salt pollution on freshwater mussel behavior, we suggest better management practices for de-icing salt use be implemented. Our experimental method demonstrated that, with the application of current technologies, mussel behavioral response to a chemical stressor can be measured. The tested sublethal endpoints are suitable for mussel ecotoxicology studies. Avoidance Behavior proved to be a potentially suitable endpoint for calculating mussel behavior effect concentration. Therefore we recommend adult mussel behavior as a suitable biomarker for future ecotoxicological research. This method could be applied to other bivalve species and for physical and environmental stressors, such as particulate matter and temperature. [Mh] Termos MeSH primário: Anodonta/fisiologia Comportamento Animal Biomarcadores/metabolismo Ecotoxicologia [Mh] Termos MeSH secundário: Análise de Variância Animais Aprendizagem da Esquiva/efeitos dos fármacos Filtração Reprodutibilidade dos Testes Cloreto de Sódio/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biomarkers); 451W47IQ8X (Sodium Chloride) [Em] Mês de entrada: 1608 [Cu] Atualização por classe: 160101 [Lr] Data última revisão: 160101 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150719 [St] Status: MEDLINE

10 / 60

MEDLINE

seleciona para imprimir Fotocópia Texto completo

[PMID]: 25208165 [Au] Autor: An C; Ouyang S; Zhou CH; Wu XP [Ad] Endereço: a Center for Watershed Ecology, Institute of Life Science, Nanchang University , Nanchang , People's Republic of China and. [Ti] Título: The complete F-type mitochondrial genome of Chinese Anodonta arcaeformis (Bivalvia: Unionidae: Anodontinae). [So] Source: Mitochondrial DNA A DNA Mapp Seq Anal;27(2):1552-3, 2016. [Is] ISSN: 2470-1408 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Anodonta arcaeformis is a Chinese common species. The complete F-type mitochondrial genome was first determined. The complete genome is 15,672 bp in length, with AT content 64.59%. All the 37 typical animal mitochondrial genes were identified, including 13 protein coding genes, 22 tRNA genes and 2 rRNA genes. And a novel FORF (277 bp, 92aa) was found between tRNA(Glu) and ND2, which was considered to be involved in sex determination. The putative control region (270 bp) is located between ND5 and tRNAGln, with an A + T content of 70.07%. The gene order is identical to other species of Unionidae female mitochondrial except Gonideinae. [Mh] Termos MeSH primário: Anodonta/genética DNA Mitocondrial/química Genoma Mitocondrial [Mh] Termos MeSH secundário: Animais Composição de Bases Mapeamento Cromossômico Ordem dos Genes Dados de Sequência Molecular Análise de Sequência de DNA [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (DNA, Mitochondrial) [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 140911 [St] Status: MEDLINE [do] DOI: 10.3109/19401736.2014.953133

---

página 1 de 6

ir para página

Refinar a pesquisa

Base de dados : MEDLINE

Formulário avançado

		Pesquisar	no campo
1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde