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Pesquisa : B01.050.500.644.116.150 [Categoria DeCS]
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[PMID]:28989097
[Au] Autor:Lindgren AR; Anderson FE
[Ad] Endereço:The Center for Life in Extreme Environments, Department of Biology, Portland State University, 1719 SW 10th Ave, SRTC Rm 246, Portland, OR 97201, USA. Electronic address: arl3@pdx.edu.
[Ti] Título:Assessing the utility of transcriptome data for inferring phylogenetic relationships among coleoid cephalopods.
[So] Source:Mol Phylogenet Evol;118:330-342, 2018 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Historically, deep-level relationships within the molluscan class Cephalopoda (squids, cuttlefishes, octopods and their relatives) have remained elusive due in part to the considerable morphological diversity of extant taxa, a limited fossil record for species that lack a calcareous shell and difficulties in sampling open ocean taxa. Many conflicts identified by morphologists in the early 1900s remain unresolved today in spite of advances in morphological, molecular and analytical methods. In this study we assess the utility of transcriptome data for resolving cephalopod phylogeny, with special focus on the orders of Decapodiformes (open-eye squids, bobtail squids, cuttlefishes and relatives). To do so, we took new and previously published transcriptome data and used a unique cephalopod core ortholog set to generate a dataset that was subjected to an array of filtering and analytical methods to assess the impacts of: taxon sampling, ortholog number, compositional and rate heterogeneity and incongruence across loci. Analyses indicated that datasets that maximized taxonomic coverage but included fewer orthologs were less stable than datasets that sacrificed taxon sampling to increase the number of orthologs. Clades recovered irrespective of dataset, filtering or analytical method included Octopodiformes (Vampyroteuthis infernalis + octopods), Decapodiformes (squids, cuttlefishes and their relatives), and orders Oegopsida (open-eyed squids) and Myopsida (e.g., loliginid squids). Ordinal-level relationships within Decapodiformes were the most susceptible to dataset perturbation, further emphasizing the challenges associated with uncovering relationships at deep nodes in the cephalopod tree of life.
[Mh] Termos MeSH primário: Cefalópodes/classificação
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
Cefalópodes/genética
Decapodiformes/classificação
Decapodiformes/genética
Fósseis
Funções Verossimilhança
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE


  2 / 3366 MEDLINE  
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[PMID]:29025669
[Au] Autor:Huang N; Lin J; Li S; Deng Y; Kong S; Hong P; Yang P; Liao M; Hu Z
[Ad] Endereço:Faculty of Chemistry and Environment Science, Guangdong Ocean University, Zhanjiang 524088, PR China. Electronic address: huangn88@126.com.
[Ti] Título:Preparation and evaluation of squid ink polysaccharide-chitosan as a wound-healing sponge.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:354-362, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A new type of wound healing agent was developed using two marine biomaterials (squid ink polysaccharide and chitosan) as carriers and calcium chloride as an initiator for coagulation. Based on central composite design-response surface methodology, comprehensive evaluation of appearance quality for composite sponges and water absorbency were used as evaluation indices to identify the optimized preparation conditions and further evaluate the performance of the squid ink polysaccharide-chitosan sponge (SIP-CS). The optimized formulation of SIP-CS was as follows: chitosan concentration, 2.29%; squid ink polysaccharide concentration, 0.55%; and calcium chloride concentration, 2.82%, at a volume ratio of 15:5:2. SIP-CS was conducive to sticking on the wound, characterized by the spongy property, strong absorptivity, and tackiness. Rabbit ear arterial, hepatic, and femoral artery hemorrhage experiments indicated that, compared with chitosan dressings and absorbable gelatin, the hemostatic times were shorter and the bleeding volume was smaller. Furthermore, SIP-CS absorbed a large amount of hemocytes, leading to rapid hemostasis. The healing areas and wound pathological sections in scalded New Zealand rabbits indicated that SIP-CS promoted wound healing more rapidly than chitosan and better than commercially available burn cream. Thus, SIP-CS is a good wound healing agent for rapid hemostasis, promoting burn/scalded skin healing, and protecting from wound infection.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Quitosana/química
Decapodiformes/metabolismo
Tinta
Polissacarídeos/química
[Mh] Termos MeSH secundário: Adsorção
Animais
Artérias/lesões
Bandagens
Materiais Biocompatíveis/farmacologia
Células Sanguíneas/citologia
Células Sanguíneas/efeitos dos fármacos
Células Sanguíneas/metabolismo
Hemorragia/prevenção & controle
Microscopia Eletrônica de Varredura
Coelhos
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Polysaccharides); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


  3 / 3366 MEDLINE  
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[PMID]:29244845
[Au] Autor:Merten V; Christiansen B; Javidpour J; Piatkowski U; Puebla O; Gasca R; Hoving HT
[Ad] Endereço:GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany.
[Ti] Título:Diet and stable isotope analyses reveal the feeding ecology of the orangeback squid Sthenoteuthis pteropus (Steenstrup 1855) (Mollusca, Ommastrephidae) in the eastern tropical Atlantic.
[So] Source:PLoS One;12(12):e0189691, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the eastern tropical Atlantic, the orangeback flying squid Sthenoteuthis pteropus (Steenstrup 1855) (Cephalopoda, Ommastrephidae) is a dominant species of the epipelagic nekton community. This carnivore squid has a short lifespan and is one of the fastest-growing squids. In this study, we characterise the role of S. pteropus in the pelagic food web of the eastern tropical Atlantic by investigating its diet and the dynamics of its feeding habits throughout its ontogeny and migration. During three expeditions in the eastern tropical Atlantic in 2015, 129 specimens were caught by hand jigging. Stomach content analyses (via visual identification and DNA barcoding) were combined with stable isotope data (∂15N and ∂13C) of muscle tissue to describe diet, feeding habits and trophic ecology of S. pteropus. Additionally, stable isotope analyses of incremental samples along the squid's gladius-the chitinous spiniform structure supporting the muscles and organs-were carried out to explore possible diet shifts through ontogeny and migration. Our results show that S. pteropus preys mainly on myctophid fishes (e.g. Myctophum asperum, Myctophum nitidulum, Vinciguerria spp.), but also on other teleost species, cephalopods (e.g. Enoploteuthidae, Bolitinidae, Ommastrephidae), crustaceans and possibly on gelatinous zooplankton as well. The squid shows a highly opportunistic feeding behaviour that includes cannibalism. Our study indicates that the trophic position of S. pteropus may increase by approximately one trophic level from a mantle length of 15 cm to 47 cm. The reconstructed isotope-based feeding chronologies of the gladii revealed high intra- and inter-individual variability in the squid's trophic position and foraging area. These findings are not revealed by diet or muscle tissue stable isotope analysis. This suggests a variable and complex life history involving individual variation and migration. The role of S. pteropus in transferring energy and nutrients from lower to higher trophic levels may be underestimated and important for understanding how a changing ocean impacts food webs in the eastern Atlantic.
[Mh] Termos MeSH primário: Decapodiformes/fisiologia
Ecologia
Comportamento Alimentar
[Mh] Termos MeSH secundário: Animais
Isótopos de Carbono
Crustáceos
Código de Barras de DNA Taxonômico
Peixes
Conteúdo Gastrointestinal/química
Isótopos de Nitrogênio
Estado Nutricional
Alimentos Marinhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbon Isotopes); 0 (Nitrogen Isotopes)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189691


  4 / 3366 MEDLINE  
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[PMID]:28941459
[Au] Autor:Dontsov AE; Sakina NL; Ostrovsky MA
[Ad] Endereço:Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334, Russia. ostrovsky3535@mail.ru.
[Ti] Título:Loss of Melanin by Eye Retinal Pigment Epithelium Cells Is Associated with Its Oxidative Destruction in Melanolipofuscin Granules.
[So] Source:Biochemistry (Mosc);82(8):916-924, 2017 Aug.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effect of superoxide radicals on melanin destruction and degradation of melanosomes isolated from cells of retinal pigment epithelium (RPE) of the human eye was studied. We found that potassium superoxide causes destruction of melanin in melanosomes of human and bovine RPE, as well as destruction of melanin from the ink bag of squid, with the formation of fluorescent decay products having an emission maximum at 520-525 nm. The initial kinetics of the accumulation of the fluorescent decay products is linear. Superoxide radicals lead simultaneously to a decrease in the number of melanosomes and to a decrease in concentration of paramagnetic centers in them. Complete degradation of melanosomes leads to the formation of a transparent solution containing dissolved proteins and melanin degradation products that do not exhibit paramagnetic properties. To completely degrade one melanosome of human RPE, 650 ± 100 fmol of superoxide are sufficient. The concentration of paramagnetic centers in a melanolipofuscin granule of human RPE is on average 32.5 ± 10.4% (p < 0.05, 150 eyes) lower than in a melanosome, which indicates melanin undergoing a destruction process in these granules. RPE cells also contain intermediate granules that have an EPR signal with a lower intensity than that of melanolipofuscin granules, but higher than that of lipofuscin granules. This signal is due to the presence of residual melanin in these granules. Irradiation of a mixture of melanosomes with lipofuscin granules with blue light (450 nm), in contrast to irradiation of only melanosomes, results in the appearance of fluorescent melanin degradation products. We suggest that one of the main mechanisms of age-related decrease in melanin concentration in human RPE cells is its destruction in melanolipofuscin granules under the action of superoxide radicals formed during photoinduced oxygen reduction by lipofuscin fluorophores.
[Mh] Termos MeSH primário: Melaninas/metabolismo
Melanossomas/metabolismo
[Mh] Termos MeSH secundário: Animais
Bovinos
Decapodiformes/metabolismo
Espectroscopia de Ressonância de Spin Eletrônica
Seres Humanos
Cinética
Luz
Lipofuscina/química
Lipofuscina/metabolismo
Melaninas/química
Melanossomas/efeitos dos fármacos
Melanossomas/efeitos da radiação
Oxirredução/efeitos dos fármacos
Oxirredução/efeitos da radiação
Epitélio Pigmentado da Retina/citologia
Epitélio Pigmentado da Retina/efeitos dos fármacos
Epitélio Pigmentado da Retina/metabolismo
Superóxidos/química
Superóxidos/metabolismo
Superóxidos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipofuscin); 0 (Melanins); 11062-77-4 (Superoxides); 12030-88-5 (potassium superoxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917080065


  5 / 3366 MEDLINE  
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[PMID]:28898600
[Au] Autor:Zepeda EA; Veline RJ; Crook RJ
[Ti] Título:Rapid Associative Learning and Stable Long-Term Memory in the Squid Euprymna scolopes.
[So] Source:Biol Bull;232(3):212-218, 2017 Jun.
[Is] ISSN:1939-8697
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Learning and memory in cephalopod molluscs have received intensive study because of cephalopods' complex behavioral repertoire and relatively accessible nervous systems. While most of this research has been conducted using octopus and cuttlefish species, there has been relatively little work on squid. Euprymna scolopes Berry, 1913, a sepiolid squid, is a promising model for further exploration of cephalopod cognition. These small squid have been studied in detail for their symbiotic relationship with bioluminescent bacteria, and their short generation time and successful captive breeding through multiple generations make them appealing models for neurobiological research. However, little is known about their behavior or cognitive ability. Using the well-established "prawn-in-the-tube" assay of learning and memory, we show that within a single 10-min trial E. scolopes learns to inhibit its predatory behavior, and after three trials it can retain this memory for at least 12 d. Rapid learning and very long-term retention were apparent under two different training schedules. To our knowledge, this study is the first demonstration of learning and memory in this species as well as the first demonstration of associative learning in any squid.
[Mh] Termos MeSH primário: Decapodiformes/fisiologia
Memória/fisiologia
[Mh] Termos MeSH secundário: Animais
Aprendizagem/fisiologia
Comportamento Predatório/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1086/693461


  6 / 3366 MEDLINE  
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[PMID]:28767686
[Au] Autor:Puneeta P; Vijai D; Yamamoto J; Adachi K; Kato Y; Sakurai Y
[Ad] Endereço:Department of Marine Bioresources and Ecology, School of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
[Ti] Título:Structure and properties of the egg mass of the ommastrephid squid Todarodes pacificus.
[So] Source:PLoS One;12(8):e0182261, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Japanese flying squid, Todarodes pacificus, is thought to spawn neutrally buoyant egg masses that retain a specific location in the water column by floating at the interface between water layers of slightly different densities. It is important to understand the physical process that determines the vertical distribution of the egg masses to predict their horizontal drift in relation to embryo survival and subsequent recruitment. Here, mesocosm experiments were conducted in a 300 m3 tank by creating a thermally stratified (17-22°C) water column to obtain egg masses. A cage net methodology was developed to sustain egg masses for detailed observation. We measured the density of the egg masses of T. pacificus, and used this information to infer the vertical distribution patterns of the egg masses at the spawning grounds (Tsushima Strait, Japan). When measured separately, the density of the outer jelly of each egg mass was 2.7 σ units higher than that of the surrounding water. The outer jelly and the specific gravity of embedded individual eggs (~1.10) cause the egg masses to have very slight negative buoyancy relative to the water in which they are formed. Analysis of the vertical profile of the spawning ground showed that water density (σθ) increased sharply at ~30 m depth; thus, egg masses might settle above the pycnocline layer. In conclusion, we suggest that T. pacificus egg masses might retain their location in the water column by floating at the interface between water layers of slightly different densities, which happen to be above the pycnocline layer (actual depth varies seasonally/annually) in the Tsushima Strait between Korea and Japan.
[Mh] Termos MeSH primário: Decapodiformes/fisiologia
Óvulo/fisiologia
[Mh] Termos MeSH secundário: Distribuição Animal
Animais
Peso Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182261


  7 / 3366 MEDLINE  
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[PMID]:28472600
[Au] Autor:Vázquez-Velasco M; González-Torres L; García-Fernández RA; Méndez MT; Bastida S; Benedí J; González-Muñoz MJ; Sánchez-Muniz FJ
[Ad] Endereço:1 Department of Nutrition and Food Science (I) Nutrition, School of Pharmacy, Complutense University , Madrid, Spain .
[Ti] Título:Glucomannan or Glucomannan Plus Spirulina-Enriched Squid-Surimi Diets Reduce Histological Damage to Liver and Heart in Zucker fa/fa Rats Fed a Cholesterol-Enriched and Non-Cholesterol-Enriched Atherogenic Diet.
[So] Source:J Med Food;20(6):618-625, 2017 Jun.
[Is] ISSN:1557-7600
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glucomannan-enriched squid surimi improves cholesterolemia and liver antioxidant status. The effect of squid surimi enriched with glucomannan or glucomannan plus spirulina on liver and heart structures and cell damage markers was tested in fa/fa rats fed highly saturated-hyper-energetic diets. Animals were fed 70% AIN-93M rodent diet plus six versions of 30% squid surimi for 7 weeks: control (C), glucomannan (G), and glucomannan plus spirulina (GS). The cholesterol-control (HC), cholesterol-glucomannan (HG), and cholesterol-glucomannan plus spirulina (HGS) groups were given similar diets that were enriched with 2% cholesterol and 0.4% cholic acid. G and GS diets versus C diet significantly inhibited weight gain and lowered plasma alanine aminotransferase and aspartate aminotransferase, liver steatosis, lipogranulomas, and total inflammation and alteration scores. The hypercholesterolemic agent significantly increased the harmful effects of the C diet. Liver weight, the hepatosomatic index, all damage markers, and total histological scoring rose for HC versus C (at least P < .05). The addition of glucomannan (HG vs. HC) improved these biomarkers, and non-additional effects from spirulina were observed except for the total liver alteration score. In conclusion, glucomannan and glucomannan plus spirulina blocked the highly saturated-hyper-energetic diet negative effects both with and without added cholesterol. Results suggest the usefulness of including these functional ingredients in fish products.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Colesterol na Dieta/efeitos adversos
Dieta Aterogênica/efeitos adversos
Produtos Pesqueiros/efeitos adversos
Fígado/metabolismo
Mananas/metabolismo
Miocárdio/metabolismo
Spirulina/metabolismo
[Mh] Termos MeSH secundário: Animais
Decapodiformes/metabolismo
Produtos Pesqueiros/análise
Coração/anatomia & histologia
Histologia
Fígado/anatomia & histologia
Masculino
Ratos
Ratos Zucker
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Cholesterol, Dietary); 0 (Mannans); 36W3E5TAMG ((1-6)-alpha-glucomannan)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1089/jmf.2016.0157


  8 / 3366 MEDLINE  
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[PMID]:28446608
[Au] Autor:Pan S; Nikolakakis K; Adamczyk PA; Pan M; Ruby EG; Reed JL
[Ad] Endereço:From the Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706.
[Ti] Título:Model-enabled gene search (MEGS) allows fast and direct discovery of enzymatic and transport gene functions in the marine bacterium .
[So] Source:J Biol Chem;292(24):10250-10261, 2017 Jun 16.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Whereas genomes can be rapidly sequenced, the functions of many genes are incompletely or erroneously annotated because of a lack of experimental evidence or prior functional knowledge in sequence databases. To address this weakness, we describe here a odel- nabled ene earch (MEGS) approach that (i) identifies metabolic functions either missing from an organism's genome annotation or incorrectly assigned to an ORF by using discrepancies between metabolic model predictions and experimental culturing data; (ii) designs functional selection experiments for these specific metabolic functions; and (iii) selects a candidate gene(s) responsible for these functions from a genomic library and directly interrogates this gene's function experimentally. To discover gene functions, MEGS uses genomic functional selections instead of relying on correlations across large experimental datasets or sequence similarity as do other approaches. When applied to the bioluminescent marine bacterium , MEGS successfully identified five genes that are responsible for four metabolic and transport reactions whose absence from a draft metabolic model of caused inaccurate modeling of high-throughput experimental data. This work demonstrates that MEGS provides a rapid and efficient integrated computational and experimental approach for annotating metabolic genes, including those that have previously been uncharacterized or misannotated.
[Mh] Termos MeSH primário: Aliivibrio fischeri/genética
Organismos Aquáticos/genética
Proteínas de Bactérias/genética
Sistemas Especialistas
Genômica/métodos
Modelos Genéticos
[Mh] Termos MeSH secundário: Aliivibrio fischeri/crescimento & desenvolvimento
Aliivibrio fischeri/metabolismo
Animais
Aquicultura
Organismos Aquáticos/metabolismo
Proteínas de Bactérias/metabolismo
Simulação por Computador
Decapodiformes/crescimento & desenvolvimento
Decapodiformes/microbiologia
Escherichia coli/genética
Escherichia coli/crescimento & desenvolvimento
Escherichia coli/metabolismo
Deleção de Genes
Teste de Complementação Genética
Biblioteca Genômica
Hawaii
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Fases de Leitura Aberta
Oceano Pacífico
Proteínas Recombinantes/metabolismo
Reprodutibilidade dos Testes
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Recombinant Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170706
[Lr] Data última revisão:
170706
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.763193


  9 / 3366 MEDLINE  
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[PMID]:28398512
[Au] Autor:Leo L; Weissmann C; Burns M; Kang M; Song Y; Qiang L; Brady ST; Baas PW; Morfini G
[Ad] Endereço:Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA, USA.
[Ti] Título:Mutant spastin proteins promote deficits in axonal transport through an isoform-specific mechanism involving casein kinase 2 activation.
[So] Source:Hum Mol Genet;26(12):2321-2334, 2017 Jun 15.
[Is] ISSN:1460-2083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mutations of various genes cause hereditary spastic paraplegia (HSP), a neurological disease involving dying-back degeneration of upper motor neurons. From these, mutations in the SPAST gene encoding the microtubule-severing protein spastin account for most HSP cases. Cumulative genetic and experimental evidence suggests that alterations in various intracellular trafficking events, including fast axonal transport (FAT), may contribute to HSP pathogenesis. However, the mechanisms linking SPAST mutations to such deficits remain largely unknown. Experiments presented here using isolated squid axoplasm reveal inhibition of FAT as a common toxic effect elicited by spastin proteins with different HSP mutations, independent of microtubule-binding or severing activity. Mutant spastin proteins produce this toxic effect only when presented as the tissue-specific M1 isoform, not when presented as the ubiquitously-expressed shorter M87 isoform. Biochemical and pharmacological experiments further indicate that the toxic effects of mutant M1 spastins on FAT involve casein kinase 2 (CK2) activation. In mammalian cells, expression of mutant M1 spastins, but not their mutant M87 counterparts, promotes abnormalities in the distribution of intracellular organelles that are correctable by pharmacological CK2 inhibition. Collectively, these results demonstrate isoform-specific toxic effects of mutant M1 spastin on FAT, and identify CK2 as a critical mediator of these effects.
[Mh] Termos MeSH primário: Adenosina Trifosfatases/genética
Transporte Axonal/genética
[Mh] Termos MeSH secundário: Adenosina Trifosfatases/metabolismo
Animais
Transporte Axonal/fisiologia
Caseína Quinase II/metabolismo
Células Cultivadas
Decapodiformes
Modelos Animais de Doenças
Fibroblastos
Seres Humanos
Microtúbulos/metabolismo
Neurônios Motores/metabolismo
Proteínas Mutantes/metabolismo
Mutação
Isoformas de Proteínas/genética
Transporte Proteico/fisiologia
Ratos
Paraplegia Espástica Hereditária/genética
Paraplegia Espástica Hereditária/metabolismo
Espastina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mutant Proteins); 0 (Protein Isoforms); EC 2.7.11.1 (Casein Kinase II); EC 3.6.1.- (Adenosine Triphosphatases); EC 3.6.4.3 (SPAST protein, human); EC 3.6.4.3 (Spastin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1093/hmg/ddx125


  10 / 3366 MEDLINE  
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[PMID]:28372201
[Au] Autor:Gu DC; Zou MJ; Guo XX; Yu P; Lin ZW; Hu T; Wu YF; Liu Y; Gan JH; Sun SQ; Wang XC; Xu CH
[Ad] Endereço:College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, PR China; Shanghai Engineering Research Center of Aquatic-Product Processing Preservation, Shanghai 201306, PR China.
[Ti] Título:A rapid analytical and quantitative evaluation of formaldehyde in squid based on Tri-step IR and partial least squares (PLS).
[So] Source:Food Chem;229:458-463, 2017 Aug 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Formaldehyde abuse for retaining squid freshness is detrimental to public health. The aim is to establish a rapid and quantitative detection method of formaldehyde in squid for screening massive samples. A linear relationship between formaldehyde concentration in squid and formaldehyde concentration in squid soaked water was observed using HPLC. Chemical profile variances of squids were rapidly analyzed by Tri-step infrared spectroscopy. Specifically, with increasing formaldehyde concentration, peak intensities of 2927cm (v (CH )), 1081cm (glycogen), 1631cm (ß-sheet proteins) decreased while 1655cm (α-helix proteins) increased. Spectral curve-fitting results further disclosed that ß-sheet proteins were transformed to α-helix and ß-turn conformations. Furthermore, a quantitative prediction model based on IR spectra was established by PLS (R , 0.9787; RMSEC, 5.51). The developed method was applicable for rapid (c.a. 5min) and quantitative analysis of formaldehyde in squids with LOD of 15mg/kg.
[Mh] Termos MeSH primário: Decapodiformes/química
Contaminação de Alimentos/análise
Formaldeído/análise
Espectrofotometria Infravermelho/métodos
[Mh] Termos MeSH secundário: Animais
Análise dos Mínimos Quadrados
Limite de Detecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE



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